human foreskin fibroblast hff  (ATCC)


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    ATCC human foreskin fibroblast hff
    Freshly harvested parasites (1 × 10 7 ) were pretreated with buffer control (NC) or 35 μg/ml of each chimeric antibody and then allowed to infect <t>HFF</t> monolayers for 2 h under normal culture conditions. The number of invasive parasites was determined using differential staining of the extracellular (red, red + green) and intracellular parasites (green) before and after detergent permeabilization. (A and B) Invasive efficacy of NC and IgG-treated parasites. Immunofluorescence microscopy showed a significant decrease in the number of invasive parasites (green) with more parasites remaining outside the cell (red, red + green) compared to NC. (C) The number of intracellular parasites was scored for 30 randomly-selected fields from two coverslips for each IgG tested and NC. (D) Tachyzoites attached to paraformaldehyde-fixed fibroblasts after incubation with the chimeric IgG and were compared to the NC. (E and F) Plaque assays of HFF monolayers infected with tachyzoites treated with plant-derived IgG antibodies (2.5 μg/ml), compared with tachyzoites treated with NC that were allowed to grow for 5 days. (G) The size of the lysis plaques was measured. Invasion levels following each antibody treatment are shown as mean percentages relative to NC ± standard deviation. Parasite attachment to host cells was scored for each antibody treatment and shown as a mean score ± standard deviation. The invasion assay was carried out in duplicate, whereas the attachment and plaque assays were carried out in triplicate. An asterisk or double asterisk denotes a statistically significant reduction in parasite invasion relative to NT (Student’s t -test, * p < 0.05; ** p < 0.01).
    Human Foreskin Fibroblast Hff, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Plant-derived chimeric antibodies inhibit the invasion of human fibroblasts by Toxoplasma gondii"

    Article Title: Plant-derived chimeric antibodies inhibit the invasion of human fibroblasts by Toxoplasma gondii

    Journal: PeerJ

    doi: 10.7717/peerj.5780

    Freshly harvested parasites (1 × 10 7 ) were pretreated with buffer control (NC) or 35 μg/ml of each chimeric antibody and then allowed to infect HFF monolayers for 2 h under normal culture conditions. The number of invasive parasites was determined using differential staining of the extracellular (red, red + green) and intracellular parasites (green) before and after detergent permeabilization. (A and B) Invasive efficacy of NC and IgG-treated parasites. Immunofluorescence microscopy showed a significant decrease in the number of invasive parasites (green) with more parasites remaining outside the cell (red, red + green) compared to NC. (C) The number of intracellular parasites was scored for 30 randomly-selected fields from two coverslips for each IgG tested and NC. (D) Tachyzoites attached to paraformaldehyde-fixed fibroblasts after incubation with the chimeric IgG and were compared to the NC. (E and F) Plaque assays of HFF monolayers infected with tachyzoites treated with plant-derived IgG antibodies (2.5 μg/ml), compared with tachyzoites treated with NC that were allowed to grow for 5 days. (G) The size of the lysis plaques was measured. Invasion levels following each antibody treatment are shown as mean percentages relative to NC ± standard deviation. Parasite attachment to host cells was scored for each antibody treatment and shown as a mean score ± standard deviation. The invasion assay was carried out in duplicate, whereas the attachment and plaque assays were carried out in triplicate. An asterisk or double asterisk denotes a statistically significant reduction in parasite invasion relative to NT (Student’s t -test, * p < 0.05; ** p < 0.01).
    Figure Legend Snippet: Freshly harvested parasites (1 × 10 7 ) were pretreated with buffer control (NC) or 35 μg/ml of each chimeric antibody and then allowed to infect HFF monolayers for 2 h under normal culture conditions. The number of invasive parasites was determined using differential staining of the extracellular (red, red + green) and intracellular parasites (green) before and after detergent permeabilization. (A and B) Invasive efficacy of NC and IgG-treated parasites. Immunofluorescence microscopy showed a significant decrease in the number of invasive parasites (green) with more parasites remaining outside the cell (red, red + green) compared to NC. (C) The number of intracellular parasites was scored for 30 randomly-selected fields from two coverslips for each IgG tested and NC. (D) Tachyzoites attached to paraformaldehyde-fixed fibroblasts after incubation with the chimeric IgG and were compared to the NC. (E and F) Plaque assays of HFF monolayers infected with tachyzoites treated with plant-derived IgG antibodies (2.5 μg/ml), compared with tachyzoites treated with NC that were allowed to grow for 5 days. (G) The size of the lysis plaques was measured. Invasion levels following each antibody treatment are shown as mean percentages relative to NC ± standard deviation. Parasite attachment to host cells was scored for each antibody treatment and shown as a mean score ± standard deviation. The invasion assay was carried out in duplicate, whereas the attachment and plaque assays were carried out in triplicate. An asterisk or double asterisk denotes a statistically significant reduction in parasite invasion relative to NT (Student’s t -test, * p < 0.05; ** p < 0.01).

    Techniques Used: Staining, Immunofluorescence, Microscopy, Incubation, Infection, Derivative Assay, Lysis, Standard Deviation, Invasion Assay

    human foreskin fibroblasts hff 2  (ATCC)


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    ATCC human foreskin fibroblasts hff 2
    Effect of Staphylococcus aureus bacteria, antibiotics (AB), and nanostructures (silver nanoparticles (AgNP), graphene oxide (GO), and their complex (AgNP-GO)) on the protein expression of proinflammatory cytokines in <t>HFF-2</t> culture cells, 3 and 6 h after treatment. The results were normalized to the negative control (NC) group. Images were created with ImageJ software. The expression of cytokines is also presented as heatmaps, where the 0 expression level is presented as dark green, and the highest observed expression (1.9 O.D.) is indicated in red.
    Human Foreskin Fibroblasts Hff 2, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Silver Nanoparticles and Graphene Oxide Complex as an Anti-Inflammatory Biocompatible Liquid Nano-Dressing for Skin Infected with Staphylococcus aureus"

    Article Title: Silver Nanoparticles and Graphene Oxide Complex as an Anti-Inflammatory Biocompatible Liquid Nano-Dressing for Skin Infected with Staphylococcus aureus

    Journal: Journal of Inflammation Research

    doi: 10.2147/JIR.S431565

    Effect of Staphylococcus aureus bacteria, antibiotics (AB), and nanostructures (silver nanoparticles (AgNP), graphene oxide (GO), and their complex (AgNP-GO)) on the protein expression of proinflammatory cytokines in HFF-2 culture cells, 3 and 6 h after treatment. The results were normalized to the negative control (NC) group. Images were created with ImageJ software. The expression of cytokines is also presented as heatmaps, where the 0 expression level is presented as dark green, and the highest observed expression (1.9 O.D.) is indicated in red.
    Figure Legend Snippet: Effect of Staphylococcus aureus bacteria, antibiotics (AB), and nanostructures (silver nanoparticles (AgNP), graphene oxide (GO), and their complex (AgNP-GO)) on the protein expression of proinflammatory cytokines in HFF-2 culture cells, 3 and 6 h after treatment. The results were normalized to the negative control (NC) group. Images were created with ImageJ software. The expression of cytokines is also presented as heatmaps, where the 0 expression level is presented as dark green, and the highest observed expression (1.9 O.D.) is indicated in red.

    Techniques Used: Bacteria, Expressing, Negative Control, Software

    human foreskin fibroblasts hffs  (ATCC)


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    ATCC human foreskin fibroblasts hffs
    VACV D10 colocalizes with mitochondria. D10 with an N-terminal 3×Flag tag was expressed from VACV during infection or a plasmid in the absence of viral infection. Under each circumstance (with or without viral infection), colocalization of D10 with mitochondria was observed in more than one cell line with more than five independent experiments. Each picture shown in the figures is representative of six different views of that particular experiment with more than 100 cells in total. In all of the cells with D10 detected (by α-Flag antibody staining), D10 colocalized with mitochondria. (A) D10 colocalizes with mitochondria in <t>HFFs</t> during VACV infection. HFFs were infected with vD10-3xFlag or WT-VACV (MOI = 3), or mock infected. Confocal microscopy was used to visualize D10 (α-Flag antibody, green), mitochondria (MitoTracker, red), and DNA (DAPI, blue) at 6, 9, and 16 hpi. (B) Zoomed-in view of the indicated areas in panel A. Asterisks indicate viral factories. (C) D10 colocalizes with mitochondria <t>in</t> <t>A549DKO</t> cells during VACV infection. A549DKO cells were infected with vD10-3xFlag or WT-VACV (MOI = 3). Confocal microscopy was employed to visualize D10 (anti-Flag antibodies, green), mitochondria (α-Tom20 antibody, red), and DNA (DAPI, blue) at 16 hpi. (D) D10 colocalizes with mitochondria in uninfected cells. A549DKO or HeLa cells were transfected with plasmid encoding codon-optimized D10 with a C-terminal 3×Flag tag. Confocal microscopy was used to visualize D10 (α-Flag antibody, green), mitochondria (MitoTracker, red), and DNA (DAPI, blue) at 24 h posttransfection.
    Human Foreskin Fibroblasts Hffs, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "A Poxvirus Decapping Enzyme Colocalizes with Mitochondria To Regulate RNA Metabolism and Translation and Promote Viral Replication"

    Article Title: A Poxvirus Decapping Enzyme Colocalizes with Mitochondria To Regulate RNA Metabolism and Translation and Promote Viral Replication

    Journal: mBio

    doi: 10.1128/mbio.00300-22

    VACV D10 colocalizes with mitochondria. D10 with an N-terminal 3×Flag tag was expressed from VACV during infection or a plasmid in the absence of viral infection. Under each circumstance (with or without viral infection), colocalization of D10 with mitochondria was observed in more than one cell line with more than five independent experiments. Each picture shown in the figures is representative of six different views of that particular experiment with more than 100 cells in total. In all of the cells with D10 detected (by α-Flag antibody staining), D10 colocalized with mitochondria. (A) D10 colocalizes with mitochondria in HFFs during VACV infection. HFFs were infected with vD10-3xFlag or WT-VACV (MOI = 3), or mock infected. Confocal microscopy was used to visualize D10 (α-Flag antibody, green), mitochondria (MitoTracker, red), and DNA (DAPI, blue) at 6, 9, and 16 hpi. (B) Zoomed-in view of the indicated areas in panel A. Asterisks indicate viral factories. (C) D10 colocalizes with mitochondria in A549DKO cells during VACV infection. A549DKO cells were infected with vD10-3xFlag or WT-VACV (MOI = 3). Confocal microscopy was employed to visualize D10 (anti-Flag antibodies, green), mitochondria (α-Tom20 antibody, red), and DNA (DAPI, blue) at 16 hpi. (D) D10 colocalizes with mitochondria in uninfected cells. A549DKO or HeLa cells were transfected with plasmid encoding codon-optimized D10 with a C-terminal 3×Flag tag. Confocal microscopy was used to visualize D10 (α-Flag antibody, green), mitochondria (MitoTracker, red), and DNA (DAPI, blue) at 24 h posttransfection.
    Figure Legend Snippet: VACV D10 colocalizes with mitochondria. D10 with an N-terminal 3×Flag tag was expressed from VACV during infection or a plasmid in the absence of viral infection. Under each circumstance (with or without viral infection), colocalization of D10 with mitochondria was observed in more than one cell line with more than five independent experiments. Each picture shown in the figures is representative of six different views of that particular experiment with more than 100 cells in total. In all of the cells with D10 detected (by α-Flag antibody staining), D10 colocalized with mitochondria. (A) D10 colocalizes with mitochondria in HFFs during VACV infection. HFFs were infected with vD10-3xFlag or WT-VACV (MOI = 3), or mock infected. Confocal microscopy was used to visualize D10 (α-Flag antibody, green), mitochondria (MitoTracker, red), and DNA (DAPI, blue) at 6, 9, and 16 hpi. (B) Zoomed-in view of the indicated areas in panel A. Asterisks indicate viral factories. (C) D10 colocalizes with mitochondria in A549DKO cells during VACV infection. A549DKO cells were infected with vD10-3xFlag or WT-VACV (MOI = 3). Confocal microscopy was employed to visualize D10 (anti-Flag antibodies, green), mitochondria (α-Tom20 antibody, red), and DNA (DAPI, blue) at 16 hpi. (D) D10 colocalizes with mitochondria in uninfected cells. A549DKO or HeLa cells were transfected with plasmid encoding codon-optimized D10 with a C-terminal 3×Flag tag. Confocal microscopy was used to visualize D10 (α-Flag antibody, green), mitochondria (MitoTracker, red), and DNA (DAPI, blue) at 24 h posttransfection.

    Techniques Used: Infection, Plasmid Preparation, Staining, Confocal Microscopy, Transfection

    human foreskin fibroblasts hffs  (ATCC)


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    ATCC human foreskin fibroblasts hffs
    (A) D10 localizes to mitochondria in <t>HFFs</t> during VACV infection. HFFs were infected with vD10-3xFlag, or WT-VACV (MOI=3), or mock-infected. Confocal microscopy was used to visualize D10 ( α -Flag antibody, green), mitochondria (MitoTracker, red), and DNA (DAPI, blue) at 6, 9, and 16 hpi (hours post-infection). (B) Zoomed in the indicated areas in A. The asterisks (*) indicate viral factories. (C) D10 localizes to mitochondria in A549DKO cells during VACV infection. A549DKO cells were infected with vD10-3xFlag or WT-VACV (MOI=3). Confocal microscopy was employed to visualize D10 (anti-Flag antibodies, green), mitochondria ( α -Tom20 antibody, red), DNA (DAPI, blue) at 16 hpi. (D) D10 localizes to mitochondria in uninfected <t>cells,</t> <t>A549</t> DKO or HeLa cells were transfected with plasmid encoding codon-optimized D10 with a C-terminal 3xFlag tag. Confocal microscopy was used to visualize D10 ( α -Flag antibody, green), mitochondria (MitoTracker, red), and DNA (DAPI, blue) at 24 hours post-transfection.
    Human Foreskin Fibroblasts Hffs, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "A poxvirus decapping enzyme localizes to mitochondria to regulate RNA metabolism and translation, and promote viral replication"

    Article Title: A poxvirus decapping enzyme localizes to mitochondria to regulate RNA metabolism and translation, and promote viral replication

    Journal: bioRxiv

    doi: 10.1101/2021.10.22.465448

    (A) D10 localizes to mitochondria in HFFs during VACV infection. HFFs were infected with vD10-3xFlag, or WT-VACV (MOI=3), or mock-infected. Confocal microscopy was used to visualize D10 ( α -Flag antibody, green), mitochondria (MitoTracker, red), and DNA (DAPI, blue) at 6, 9, and 16 hpi (hours post-infection). (B) Zoomed in the indicated areas in A. The asterisks (*) indicate viral factories. (C) D10 localizes to mitochondria in A549DKO cells during VACV infection. A549DKO cells were infected with vD10-3xFlag or WT-VACV (MOI=3). Confocal microscopy was employed to visualize D10 (anti-Flag antibodies, green), mitochondria ( α -Tom20 antibody, red), DNA (DAPI, blue) at 16 hpi. (D) D10 localizes to mitochondria in uninfected cells, A549 DKO or HeLa cells were transfected with plasmid encoding codon-optimized D10 with a C-terminal 3xFlag tag. Confocal microscopy was used to visualize D10 ( α -Flag antibody, green), mitochondria (MitoTracker, red), and DNA (DAPI, blue) at 24 hours post-transfection.
    Figure Legend Snippet: (A) D10 localizes to mitochondria in HFFs during VACV infection. HFFs were infected with vD10-3xFlag, or WT-VACV (MOI=3), or mock-infected. Confocal microscopy was used to visualize D10 ( α -Flag antibody, green), mitochondria (MitoTracker, red), and DNA (DAPI, blue) at 6, 9, and 16 hpi (hours post-infection). (B) Zoomed in the indicated areas in A. The asterisks (*) indicate viral factories. (C) D10 localizes to mitochondria in A549DKO cells during VACV infection. A549DKO cells were infected with vD10-3xFlag or WT-VACV (MOI=3). Confocal microscopy was employed to visualize D10 (anti-Flag antibodies, green), mitochondria ( α -Tom20 antibody, red), DNA (DAPI, blue) at 16 hpi. (D) D10 localizes to mitochondria in uninfected cells, A549 DKO or HeLa cells were transfected with plasmid encoding codon-optimized D10 with a C-terminal 3xFlag tag. Confocal microscopy was used to visualize D10 ( α -Flag antibody, green), mitochondria (MitoTracker, red), and DNA (DAPI, blue) at 24 hours post-transfection.

    Techniques Used: Infection, Confocal Microscopy, Transfection, Plasmid Preparation

    human foreskin fibroblast hff  (ATCC)


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    ATCC human foreskin fibroblast hff
    Freshly harvested parasites (1 × 10 7 ) were pretreated with buffer control (NC) or 35 μg/ml of each chimeric antibody and then allowed to infect <t>HFF</t> monolayers for 2 h under normal culture conditions. The number of invasive parasites was determined using differential staining of the extracellular (red, red + green) and intracellular parasites (green) before and after detergent permeabilization. (A and B) Invasive efficacy of NC and IgG-treated parasites. Immunofluorescence microscopy showed a significant decrease in the number of invasive parasites (green) with more parasites remaining outside the cell (red, red + green) compared to NC. (C) The number of intracellular parasites was scored for 30 randomly-selected fields from two coverslips for each IgG tested and NC. (D) Tachyzoites attached to paraformaldehyde-fixed fibroblasts after incubation with the chimeric IgG and were compared to the NC. (E and F) Plaque assays of HFF monolayers infected with tachyzoites treated with plant-derived IgG antibodies (2.5 μg/ml), compared with tachyzoites treated with NC that were allowed to grow for 5 days. (G) The size of the lysis plaques was measured. Invasion levels following each antibody treatment are shown as mean percentages relative to NC ± standard deviation. Parasite attachment to host cells was scored for each antibody treatment and shown as a mean score ± standard deviation. The invasion assay was carried out in duplicate, whereas the attachment and plaque assays were carried out in triplicate. An asterisk or double asterisk denotes a statistically significant reduction in parasite invasion relative to NT (Student’s t -test, * p < 0.05; ** p < 0.01).
    Human Foreskin Fibroblast Hff, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Plant-derived chimeric antibodies inhibit the invasion of human fibroblasts by Toxoplasma gondii"

    Article Title: Plant-derived chimeric antibodies inhibit the invasion of human fibroblasts by Toxoplasma gondii

    Journal: PeerJ

    doi: 10.7717/peerj.5780

    Freshly harvested parasites (1 × 10 7 ) were pretreated with buffer control (NC) or 35 μg/ml of each chimeric antibody and then allowed to infect HFF monolayers for 2 h under normal culture conditions. The number of invasive parasites was determined using differential staining of the extracellular (red, red + green) and intracellular parasites (green) before and after detergent permeabilization. (A and B) Invasive efficacy of NC and IgG-treated parasites. Immunofluorescence microscopy showed a significant decrease in the number of invasive parasites (green) with more parasites remaining outside the cell (red, red + green) compared to NC. (C) The number of intracellular parasites was scored for 30 randomly-selected fields from two coverslips for each IgG tested and NC. (D) Tachyzoites attached to paraformaldehyde-fixed fibroblasts after incubation with the chimeric IgG and were compared to the NC. (E and F) Plaque assays of HFF monolayers infected with tachyzoites treated with plant-derived IgG antibodies (2.5 μg/ml), compared with tachyzoites treated with NC that were allowed to grow for 5 days. (G) The size of the lysis plaques was measured. Invasion levels following each antibody treatment are shown as mean percentages relative to NC ± standard deviation. Parasite attachment to host cells was scored for each antibody treatment and shown as a mean score ± standard deviation. The invasion assay was carried out in duplicate, whereas the attachment and plaque assays were carried out in triplicate. An asterisk or double asterisk denotes a statistically significant reduction in parasite invasion relative to NT (Student’s t -test, * p < 0.05; ** p < 0.01).
    Figure Legend Snippet: Freshly harvested parasites (1 × 10 7 ) were pretreated with buffer control (NC) or 35 μg/ml of each chimeric antibody and then allowed to infect HFF monolayers for 2 h under normal culture conditions. The number of invasive parasites was determined using differential staining of the extracellular (red, red + green) and intracellular parasites (green) before and after detergent permeabilization. (A and B) Invasive efficacy of NC and IgG-treated parasites. Immunofluorescence microscopy showed a significant decrease in the number of invasive parasites (green) with more parasites remaining outside the cell (red, red + green) compared to NC. (C) The number of intracellular parasites was scored for 30 randomly-selected fields from two coverslips for each IgG tested and NC. (D) Tachyzoites attached to paraformaldehyde-fixed fibroblasts after incubation with the chimeric IgG and were compared to the NC. (E and F) Plaque assays of HFF monolayers infected with tachyzoites treated with plant-derived IgG antibodies (2.5 μg/ml), compared with tachyzoites treated with NC that were allowed to grow for 5 days. (G) The size of the lysis plaques was measured. Invasion levels following each antibody treatment are shown as mean percentages relative to NC ± standard deviation. Parasite attachment to host cells was scored for each antibody treatment and shown as a mean score ± standard deviation. The invasion assay was carried out in duplicate, whereas the attachment and plaque assays were carried out in triplicate. An asterisk or double asterisk denotes a statistically significant reduction in parasite invasion relative to NT (Student’s t -test, * p < 0.05; ** p < 0.01).

    Techniques Used: Staining, Immunofluorescence, Microscopy, Incubation, Infection, Derivative Assay, Lysis, Standard Deviation, Invasion Assay

    human foreskin fibroblasts hff 2  (ATCC)


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    ATCC human foreskin fibroblasts hff 2
    Human Foreskin Fibroblasts Hff 2, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human foreskin fibroblast hff cells  (ATCC)


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    ATCC human foreskin fibroblast hff cells
    Human Foreskin Fibroblast Hff Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human foreskin fibroblast hff cells  (ATCC)


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    ATCC human foreskin fibroblast hff cells
    Human Foreskin Fibroblast Hff Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human foreskin fibroblasts hff 103 cells  (ATCC)


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    ATCC human foreskin fibroblasts hff 103 cells
    Human Foreskin Fibroblasts Hff 103 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC human foreskin fibroblast hff
    Freshly harvested parasites (1 × 10 7 ) were pretreated with buffer control (NC) or 35 μg/ml of each chimeric antibody and then allowed to infect <t>HFF</t> monolayers for 2 h under normal culture conditions. The number of invasive parasites was determined using differential staining of the extracellular (red, red + green) and intracellular parasites (green) before and after detergent permeabilization. (A and B) Invasive efficacy of NC and IgG-treated parasites. Immunofluorescence microscopy showed a significant decrease in the number of invasive parasites (green) with more parasites remaining outside the cell (red, red + green) compared to NC. (C) The number of intracellular parasites was scored for 30 randomly-selected fields from two coverslips for each IgG tested and NC. (D) Tachyzoites attached to paraformaldehyde-fixed fibroblasts after incubation with the chimeric IgG and were compared to the NC. (E and F) Plaque assays of HFF monolayers infected with tachyzoites treated with plant-derived IgG antibodies (2.5 μg/ml), compared with tachyzoites treated with NC that were allowed to grow for 5 days. (G) The size of the lysis plaques was measured. Invasion levels following each antibody treatment are shown as mean percentages relative to NC ± standard deviation. Parasite attachment to host cells was scored for each antibody treatment and shown as a mean score ± standard deviation. The invasion assay was carried out in duplicate, whereas the attachment and plaque assays were carried out in triplicate. An asterisk or double asterisk denotes a statistically significant reduction in parasite invasion relative to NT (Student’s t -test, * p < 0.05; ** p < 0.01).
    Human Foreskin Fibroblast Hff, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 1 article reviews
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    86
    ATCC human foreskin fibroblasts hff 2
    Effect of Staphylococcus aureus bacteria, antibiotics (AB), and nanostructures (silver nanoparticles (AgNP), graphene oxide (GO), and their complex (AgNP-GO)) on the protein expression of proinflammatory cytokines in <t>HFF-2</t> culture cells, 3 and 6 h after treatment. The results were normalized to the negative control (NC) group. Images were created with ImageJ software. The expression of cytokines is also presented as heatmaps, where the 0 expression level is presented as dark green, and the highest observed expression (1.9 O.D.) is indicated in red.
    Human Foreskin Fibroblasts Hff 2, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    96
    ATCC human foreskin fibroblasts hffs
    VACV D10 colocalizes with mitochondria. D10 with an N-terminal 3×Flag tag was expressed from VACV during infection or a plasmid in the absence of viral infection. Under each circumstance (with or without viral infection), colocalization of D10 with mitochondria was observed in more than one cell line with more than five independent experiments. Each picture shown in the figures is representative of six different views of that particular experiment with more than 100 cells in total. In all of the cells with D10 detected (by α-Flag antibody staining), D10 colocalized with mitochondria. (A) D10 colocalizes with mitochondria in <t>HFFs</t> during VACV infection. HFFs were infected with vD10-3xFlag or WT-VACV (MOI = 3), or mock infected. Confocal microscopy was used to visualize D10 (α-Flag antibody, green), mitochondria (MitoTracker, red), and DNA (DAPI, blue) at 6, 9, and 16 hpi. (B) Zoomed-in view of the indicated areas in panel A. Asterisks indicate viral factories. (C) D10 colocalizes with mitochondria <t>in</t> <t>A549DKO</t> cells during VACV infection. A549DKO cells were infected with vD10-3xFlag or WT-VACV (MOI = 3). Confocal microscopy was employed to visualize D10 (anti-Flag antibodies, green), mitochondria (α-Tom20 antibody, red), and DNA (DAPI, blue) at 16 hpi. (D) D10 colocalizes with mitochondria in uninfected cells. A549DKO or HeLa cells were transfected with plasmid encoding codon-optimized D10 with a C-terminal 3×Flag tag. Confocal microscopy was used to visualize D10 (α-Flag antibody, green), mitochondria (MitoTracker, red), and DNA (DAPI, blue) at 24 h posttransfection.
    Human Foreskin Fibroblasts Hffs, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human foreskin fibroblasts hffs/product/ATCC
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    99
    ATCC human foreskin fibroblast hff cells
    VACV D10 colocalizes with mitochondria. D10 with an N-terminal 3×Flag tag was expressed from VACV during infection or a plasmid in the absence of viral infection. Under each circumstance (with or without viral infection), colocalization of D10 with mitochondria was observed in more than one cell line with more than five independent experiments. Each picture shown in the figures is representative of six different views of that particular experiment with more than 100 cells in total. In all of the cells with D10 detected (by α-Flag antibody staining), D10 colocalized with mitochondria. (A) D10 colocalizes with mitochondria in <t>HFFs</t> during VACV infection. HFFs were infected with vD10-3xFlag or WT-VACV (MOI = 3), or mock infected. Confocal microscopy was used to visualize D10 (α-Flag antibody, green), mitochondria (MitoTracker, red), and DNA (DAPI, blue) at 6, 9, and 16 hpi. (B) Zoomed-in view of the indicated areas in panel A. Asterisks indicate viral factories. (C) D10 colocalizes with mitochondria <t>in</t> <t>A549DKO</t> cells during VACV infection. A549DKO cells were infected with vD10-3xFlag or WT-VACV (MOI = 3). Confocal microscopy was employed to visualize D10 (anti-Flag antibodies, green), mitochondria (α-Tom20 antibody, red), and DNA (DAPI, blue) at 16 hpi. (D) D10 colocalizes with mitochondria in uninfected cells. A549DKO or HeLa cells were transfected with plasmid encoding codon-optimized D10 with a C-terminal 3×Flag tag. Confocal microscopy was used to visualize D10 (α-Flag antibody, green), mitochondria (MitoTracker, red), and DNA (DAPI, blue) at 24 h posttransfection.
    Human Foreskin Fibroblast Hff Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human foreskin fibroblast hff cells/product/ATCC
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    99
    ATCC human foreskin fibroblasts hff 103 cells
    VACV D10 colocalizes with mitochondria. D10 with an N-terminal 3×Flag tag was expressed from VACV during infection or a plasmid in the absence of viral infection. Under each circumstance (with or without viral infection), colocalization of D10 with mitochondria was observed in more than one cell line with more than five independent experiments. Each picture shown in the figures is representative of six different views of that particular experiment with more than 100 cells in total. In all of the cells with D10 detected (by α-Flag antibody staining), D10 colocalized with mitochondria. (A) D10 colocalizes with mitochondria in <t>HFFs</t> during VACV infection. HFFs were infected with vD10-3xFlag or WT-VACV (MOI = 3), or mock infected. Confocal microscopy was used to visualize D10 (α-Flag antibody, green), mitochondria (MitoTracker, red), and DNA (DAPI, blue) at 6, 9, and 16 hpi. (B) Zoomed-in view of the indicated areas in panel A. Asterisks indicate viral factories. (C) D10 colocalizes with mitochondria <t>in</t> <t>A549DKO</t> cells during VACV infection. A549DKO cells were infected with vD10-3xFlag or WT-VACV (MOI = 3). Confocal microscopy was employed to visualize D10 (anti-Flag antibodies, green), mitochondria (α-Tom20 antibody, red), and DNA (DAPI, blue) at 16 hpi. (D) D10 colocalizes with mitochondria in uninfected cells. A549DKO or HeLa cells were transfected with plasmid encoding codon-optimized D10 with a C-terminal 3×Flag tag. Confocal microscopy was used to visualize D10 (α-Flag antibody, green), mitochondria (MitoTracker, red), and DNA (DAPI, blue) at 24 h posttransfection.
    Human Foreskin Fibroblasts Hff 103 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Freshly harvested parasites (1 × 10 7 ) were pretreated with buffer control (NC) or 35 μg/ml of each chimeric antibody and then allowed to infect HFF monolayers for 2 h under normal culture conditions. The number of invasive parasites was determined using differential staining of the extracellular (red, red + green) and intracellular parasites (green) before and after detergent permeabilization. (A and B) Invasive efficacy of NC and IgG-treated parasites. Immunofluorescence microscopy showed a significant decrease in the number of invasive parasites (green) with more parasites remaining outside the cell (red, red + green) compared to NC. (C) The number of intracellular parasites was scored for 30 randomly-selected fields from two coverslips for each IgG tested and NC. (D) Tachyzoites attached to paraformaldehyde-fixed fibroblasts after incubation with the chimeric IgG and were compared to the NC. (E and F) Plaque assays of HFF monolayers infected with tachyzoites treated with plant-derived IgG antibodies (2.5 μg/ml), compared with tachyzoites treated with NC that were allowed to grow for 5 days. (G) The size of the lysis plaques was measured. Invasion levels following each antibody treatment are shown as mean percentages relative to NC ± standard deviation. Parasite attachment to host cells was scored for each antibody treatment and shown as a mean score ± standard deviation. The invasion assay was carried out in duplicate, whereas the attachment and plaque assays were carried out in triplicate. An asterisk or double asterisk denotes a statistically significant reduction in parasite invasion relative to NT (Student’s t -test, * p < 0.05; ** p < 0.01).

    Journal: PeerJ

    Article Title: Plant-derived chimeric antibodies inhibit the invasion of human fibroblasts by Toxoplasma gondii

    doi: 10.7717/peerj.5780

    Figure Lengend Snippet: Freshly harvested parasites (1 × 10 7 ) were pretreated with buffer control (NC) or 35 μg/ml of each chimeric antibody and then allowed to infect HFF monolayers for 2 h under normal culture conditions. The number of invasive parasites was determined using differential staining of the extracellular (red, red + green) and intracellular parasites (green) before and after detergent permeabilization. (A and B) Invasive efficacy of NC and IgG-treated parasites. Immunofluorescence microscopy showed a significant decrease in the number of invasive parasites (green) with more parasites remaining outside the cell (red, red + green) compared to NC. (C) The number of intracellular parasites was scored for 30 randomly-selected fields from two coverslips for each IgG tested and NC. (D) Tachyzoites attached to paraformaldehyde-fixed fibroblasts after incubation with the chimeric IgG and were compared to the NC. (E and F) Plaque assays of HFF monolayers infected with tachyzoites treated with plant-derived IgG antibodies (2.5 μg/ml), compared with tachyzoites treated with NC that were allowed to grow for 5 days. (G) The size of the lysis plaques was measured. Invasion levels following each antibody treatment are shown as mean percentages relative to NC ± standard deviation. Parasite attachment to host cells was scored for each antibody treatment and shown as a mean score ± standard deviation. The invasion assay was carried out in duplicate, whereas the attachment and plaque assays were carried out in triplicate. An asterisk or double asterisk denotes a statistically significant reduction in parasite invasion relative to NT (Student’s t -test, * p < 0.05; ** p < 0.01).

    Article Snippet: The Hs68 fibroblast cell line derived from human foreskin fibroblast (HFF) was obtained from ATCC (CRL-1446).

    Techniques: Staining, Immunofluorescence, Microscopy, Incubation, Infection, Derivative Assay, Lysis, Standard Deviation, Invasion Assay

    Effect of Staphylococcus aureus bacteria, antibiotics (AB), and nanostructures (silver nanoparticles (AgNP), graphene oxide (GO), and their complex (AgNP-GO)) on the protein expression of proinflammatory cytokines in HFF-2 culture cells, 3 and 6 h after treatment. The results were normalized to the negative control (NC) group. Images were created with ImageJ software. The expression of cytokines is also presented as heatmaps, where the 0 expression level is presented as dark green, and the highest observed expression (1.9 O.D.) is indicated in red.

    Journal: Journal of Inflammation Research

    Article Title: Silver Nanoparticles and Graphene Oxide Complex as an Anti-Inflammatory Biocompatible Liquid Nano-Dressing for Skin Infected with Staphylococcus aureus

    doi: 10.2147/JIR.S431565

    Figure Lengend Snippet: Effect of Staphylococcus aureus bacteria, antibiotics (AB), and nanostructures (silver nanoparticles (AgNP), graphene oxide (GO), and their complex (AgNP-GO)) on the protein expression of proinflammatory cytokines in HFF-2 culture cells, 3 and 6 h after treatment. The results were normalized to the negative control (NC) group. Images were created with ImageJ software. The expression of cytokines is also presented as heatmaps, where the 0 expression level is presented as dark green, and the highest observed expression (1.9 O.D.) is indicated in red.

    Article Snippet: To confirm the influence of the tested factors and bacteria on inflammatory status, we utilized a second biological model, similar to human skin, which was human foreskin fibroblasts (HFF-2) (ATCC, Manassas, VA, USA).

    Techniques: Bacteria, Expressing, Negative Control, Software

    VACV D10 colocalizes with mitochondria. D10 with an N-terminal 3×Flag tag was expressed from VACV during infection or a plasmid in the absence of viral infection. Under each circumstance (with or without viral infection), colocalization of D10 with mitochondria was observed in more than one cell line with more than five independent experiments. Each picture shown in the figures is representative of six different views of that particular experiment with more than 100 cells in total. In all of the cells with D10 detected (by α-Flag antibody staining), D10 colocalized with mitochondria. (A) D10 colocalizes with mitochondria in HFFs during VACV infection. HFFs were infected with vD10-3xFlag or WT-VACV (MOI = 3), or mock infected. Confocal microscopy was used to visualize D10 (α-Flag antibody, green), mitochondria (MitoTracker, red), and DNA (DAPI, blue) at 6, 9, and 16 hpi. (B) Zoomed-in view of the indicated areas in panel A. Asterisks indicate viral factories. (C) D10 colocalizes with mitochondria in A549DKO cells during VACV infection. A549DKO cells were infected with vD10-3xFlag or WT-VACV (MOI = 3). Confocal microscopy was employed to visualize D10 (anti-Flag antibodies, green), mitochondria (α-Tom20 antibody, red), and DNA (DAPI, blue) at 16 hpi. (D) D10 colocalizes with mitochondria in uninfected cells. A549DKO or HeLa cells were transfected with plasmid encoding codon-optimized D10 with a C-terminal 3×Flag tag. Confocal microscopy was used to visualize D10 (α-Flag antibody, green), mitochondria (MitoTracker, red), and DNA (DAPI, blue) at 24 h posttransfection.

    Journal: mBio

    Article Title: A Poxvirus Decapping Enzyme Colocalizes with Mitochondria To Regulate RNA Metabolism and Translation and Promote Viral Replication

    doi: 10.1128/mbio.00300-22

    Figure Lengend Snippet: VACV D10 colocalizes with mitochondria. D10 with an N-terminal 3×Flag tag was expressed from VACV during infection or a plasmid in the absence of viral infection. Under each circumstance (with or without viral infection), colocalization of D10 with mitochondria was observed in more than one cell line with more than five independent experiments. Each picture shown in the figures is representative of six different views of that particular experiment with more than 100 cells in total. In all of the cells with D10 detected (by α-Flag antibody staining), D10 colocalized with mitochondria. (A) D10 colocalizes with mitochondria in HFFs during VACV infection. HFFs were infected with vD10-3xFlag or WT-VACV (MOI = 3), or mock infected. Confocal microscopy was used to visualize D10 (α-Flag antibody, green), mitochondria (MitoTracker, red), and DNA (DAPI, blue) at 6, 9, and 16 hpi. (B) Zoomed-in view of the indicated areas in panel A. Asterisks indicate viral factories. (C) D10 colocalizes with mitochondria in A549DKO cells during VACV infection. A549DKO cells were infected with vD10-3xFlag or WT-VACV (MOI = 3). Confocal microscopy was employed to visualize D10 (anti-Flag antibodies, green), mitochondria (α-Tom20 antibody, red), and DNA (DAPI, blue) at 16 hpi. (D) D10 colocalizes with mitochondria in uninfected cells. A549DKO or HeLa cells were transfected with plasmid encoding codon-optimized D10 with a C-terminal 3×Flag tag. Confocal microscopy was used to visualize D10 (α-Flag antibody, green), mitochondria (MitoTracker, red), and DNA (DAPI, blue) at 24 h posttransfection.

    Article Snippet: A549 control cells and A549DKO cells (kind gifts from Bernard Moss) , human foreskin fibroblasts (HFFs) (a kind gift from Nicholas Wallace), HeLa cells (ATCC CCL-2), 293T cells (ATCC CRL-3216), and BHK-21 cells (C-13) were cultured in Dulbecco’s minimal essential medium (DMEM; Quality Biological).

    Techniques: Infection, Plasmid Preparation, Staining, Confocal Microscopy, Transfection