Journal: Scientific Reports

Article Title: The study of tryptophol containing emulgel on fungal reduction and skin irritation

doi: 10.1038/s41598-023-46121-z

Figure Lengend Snippet: Cell viability ( a ) and cytotoxicity ( b ) of TOH-treated human foreskin fibroblasts (HFF- 1) cells at 37 °C as determined by MTT, Mitogreen, LDH, and dual AO/EB staining assays. Percentages of cell viability and cytotoxicity were shown as mean ± SD. *P < 0.05 and ****P < 0.0001 indicate significant differences compared with the control group (concentration 0). TOH Tryptophol, VOZ voriconazole.

Article Snippet: Human foreskin fibroblasts (HFF-1) cell line (ATCC #SCRC-1041™) was used to investigate cell viability and cytotoxicity after TOH treatments.

Techniques: Staining, Concentration Assay

Journal: Current Issues in Molecular Biology

Article Title: Extremophilic Solutions: The Role of Deinoxanthin in Counteracting UV-Induced Skin Harm

doi: 10.3390/cimb45100528

Figure Lengend Snippet: Effects of varying DNX concentrations on the cytotoxicity of human foreskin fibroblast cells (HFF-1) observed at ( A ) 24 h and ( B ) 48 h. Values are represented as mean ± standard error of mean. * p < 0.05 vs. 0 µM DNX groups.

Article Snippet: The human foreskin fibroblast cell line HFF-1 was obtained from ATCC (American Type Culture Collection, Manassas, VA, USA, LGC Promochem, Teddington, UK).

Techniques:

Journal: Current Issues in Molecular Biology

Article Title: Extremophilic Solutions: The Role of Deinoxanthin in Counteracting UV-Induced Skin Harm

doi: 10.3390/cimb45100528

Figure Lengend Snippet: Protective effects of varying concentrations of DNX on HFF-1 cells exposed to UVB and UVA radiation observed at ( A ) UVA and ( B ) UVB. Statistical significance in this figure indicates the observed differences between the control group and the groups treated with varying concentrations of DNX and VitC, all subjected to the same UV dosage levels. The results are presented as the mean ± s.e.m. ( n = 6). * p < 0.05; ** p < 0.001; *** p < 0.005.

Article Snippet: The human foreskin fibroblast cell line HFF-1 was obtained from ATCC (American Type Culture Collection, Manassas, VA, USA, LGC Promochem, Teddington, UK).

Techniques:

Journal: Current Issues in Molecular Biology

Article Title: Extremophilic Solutions: The Role of Deinoxanthin in Counteracting UV-Induced Skin Harm

doi: 10.3390/cimb45100528

Figure Lengend Snippet: Evaluation of apoptosis-associated parameters in HFF-1 cells: viable and apoptotic cells were quantified. Each group used three wells for Annexin V-FITC/PI staining. Population analyses were performed in triplicate, and the entire assay was repeated three times.

Article Snippet: The human foreskin fibroblast cell line HFF-1 was obtained from ATCC (American Type Culture Collection, Manassas, VA, USA, LGC Promochem, Teddington, UK).

Techniques: Staining

Journal: Current Issues in Molecular Biology

Article Title: Extremophilic Solutions: The Role of Deinoxanthin in Counteracting UV-Induced Skin Harm

doi: 10.3390/cimb45100528

Figure Lengend Snippet: Activity of tyrosinase in HFF-1 cells exposed to UVA ( A ) and UVB ( B ) radiation was determined after treatment with varying concentrations of DNX. Statistical significance in this figure indicates the observed differences between the control group and the groups treated with varying concentrations of DNX and VitC, all subjected to the same UV dosage levels. The mean percentages ± s.e.m. are shown ( n = 6). * p < 0.05; ** p < 0.001; *** p < 0.005.

Article Snippet: The human foreskin fibroblast cell line HFF-1 was obtained from ATCC (American Type Culture Collection, Manassas, VA, USA, LGC Promochem, Teddington, UK).

Techniques: Activity Assay

Journal: Current Issues in Molecular Biology

Article Title: Extremophilic Solutions: The Role of Deinoxanthin in Counteracting UV-Induced Skin Harm

doi: 10.3390/cimb45100528

Figure Lengend Snippet: Activity of SOD in HFF-1 cell after UVA and UVB irradiation with different concentrations of DNX and VitC. Values are represented as mean ± standard error of mean. a p < 0.05 vs. control group. b p < 0.05 vs. UVA group. c p < 0.05 VitC-treated + UVA group. d p < 0.05 UVB group. e p < 0.05 VitC-treated + UVB group.

Article Snippet: The human foreskin fibroblast cell line HFF-1 was obtained from ATCC (American Type Culture Collection, Manassas, VA, USA, LGC Promochem, Teddington, UK).

Techniques: Activity Assay, Irradiation

Journal: Current Issues in Molecular Biology

Article Title: Extremophilic Solutions: The Role of Deinoxanthin in Counteracting UV-Induced Skin Harm

doi: 10.3390/cimb45100528

Figure Lengend Snippet: ( A ) ROS level in HFF-1 cells post UVA and UVB exposure, influenced by varying concentrations of DNX ( B ) MDA in HFF-1 cell after UVA and UVB irradiation with varying concentrations of DNX. Values are represented as mean ± standard error of mean. a p < 0.05 vs. control group. b p < 0.05 vs. UVA group. c p < 0.05 VitC-treated + UVA group. d p < 0.05 UVB group. e p < 0.05 VitC-treated + UVB group.

Article Snippet: The human foreskin fibroblast cell line HFF-1 was obtained from ATCC (American Type Culture Collection, Manassas, VA, USA, LGC Promochem, Teddington, UK).

Techniques: Irradiation

Journal: Current Issues in Molecular Biology

Article Title: Extremophilic Solutions: The Role of Deinoxanthin in Counteracting UV-Induced Skin Harm

doi: 10.3390/cimb45100528

Figure Lengend Snippet: Hydroxyproline formation in HFF-1 cell after UVA and UVB irradiation with varying concentrations of DNX and VitC. Values are represented as mean ± standard error of mean. a p < 0.05 vs. control group. b p < 0.05 vs. UVA group. c p < 0.05 VitC-treated + UVA group. d p < 0.05 UVB group. e p < 0.05 VitC-treated + UVB group.

Article Snippet: The human foreskin fibroblast cell line HFF-1 was obtained from ATCC (American Type Culture Collection, Manassas, VA, USA, LGC Promochem, Teddington, UK).

Techniques: Irradiation

Journal: Current Issues in Molecular Biology

Article Title: Extremophilic Solutions: The Role of Deinoxanthin in Counteracting UV-Induced Skin Harm

doi: 10.3390/cimb45100528

Figure Lengend Snippet: Impact of DNX on the wound healing response in UVA-exposed HFF-1 cells. ( A ) DNX counteracts the impediments in wound healing caused by UVA exposure. Upon reaching confluency, cells were scratched using a pipette tip and subsequently pretreated with DNX and VitC before UVA exposure. Inverted images (40× magnifications, 400 µm scale bar) of the scratched HFF-1 cells were captured at the 0 and 24 h marks post irradiation. ( B ) The wound healing assay’s graph, representing % wound closure, was generated by analyzing the captured inverted images through the Image-Pro Plus 6.0 Software. Data are expressed as the mean ± standard deviation of three independent experiments. Ns: non-significance, * p < 0.05.

Article Snippet: The human foreskin fibroblast cell line HFF-1 was obtained from ATCC (American Type Culture Collection, Manassas, VA, USA, LGC Promochem, Teddington, UK).

Techniques: Transferring, Irradiation, Generated, Software, Standard Deviation

Journal: Current Issues in Molecular Biology

Article Title: Extremophilic Solutions: The Role of Deinoxanthin in Counteracting UV-Induced Skin Harm

doi: 10.3390/cimb45100528

Figure Lengend Snippet: Effect of DNX on scratch wound healing in UVB-irradiated HFF-1 cells. ( A ) DNX rescues the UVB-induced wound-healing defect. Confluent cells were mechanically scratched with a pipette tip and pretreated with DNX and VitC prior to UVB irradiation. Invert micrographs (40× magnifications, 400 µm scale bar) of scratched HFF-1 cells were taken at 0 and 24 h following irradiation. ( B ) In the wound healing assay, the graph of % wound closure was derived from the assessment of inverted micrographs using Image-Pro Plus 6.0 Software. Data are expressed as the mean ± standard deviation of three independent experiments. ns: non-significance, * p < 0.05.

Article Snippet: The human foreskin fibroblast cell line HFF-1 was obtained from ATCC (American Type Culture Collection, Manassas, VA, USA, LGC Promochem, Teddington, UK).

Techniques: Irradiation, Transferring, Wound Healing Assay, Derivative Assay, Software, Standard Deviation

Journal: International Journal of Molecular Sciences

Article Title: Arginine Is a Novel Drug Target for Arginine Decarboxylase in Human Colorectal Cancer Cells

doi: 10.3390/ijms241813741

Figure Lengend Snippet: ADC performed better than ADI in ASS-positive human colorectal cancer cell lines and was less toxic to normal cells. ( A ) Immunoblot analysis showing the expression of ASS in colorectal cancer cells. ( B ) Cell viability towards ADI of different cancer cell lines. ( C ) Cell viability towards ADC of different cancer cell lines and normal human fibroblast HFF1. ( D ) Bar chart comparing the effects of AFM and 100 μg/mL ADC on the viability of HCT116 and COLO 205 cells. Cells were treated for 72 h before MTT analysis. ( E ) Cell viability towards ADC in rat primary hepatocytes. Data are expressed as the percentage of viable cells compared to control (complete medium) in the form of mean ± SEM of three individual experiments. * p < 0.05, ** p < 0.01, *** p < 0.001 using Mann–Whitney U test (versus control). # p < 0.05, ## p < 0.01, ### p < 0.001 using one-way ANOVA followed by Bonferroni test (between groups).

Article Snippet: Human foreskin fibroblast cell line HFF1 was purchased from the American Type Culture Collection (Manassas, VA, U.S.).

Techniques: Western Blot, Expressing, MANN-WHITNEY