human fibronectin  (Thermo Fisher)


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    Name:
    Fibronectin human Native Protein
    Description:
    Fibronectin human Native Protein for Ctrl
    Catalog Number:
    RP43130
    Price:
    None
    Category:
    Proteins Enzymes Peptides
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    Structured Review

    Thermo Fisher human fibronectin
    Changes in ZP on the surface of the nanocrystalline pellet measured post flow for (A) LCs with a base fibronection coating, (B) LCs seeded with endothelial cells, (C) MNs with a base <t>fibronectin</t> coating, and (D) MNs seeded with endothelial cells. Paired t ‐test, n = 3 for all conditions. *** p
    Fibronectin human Native Protein for Ctrl
    https://www.bioz.com/result/human fibronectin/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human fibronectin - by Bioz Stars, 2021-04
    93/100 stars

    Images

    1) Product Images from "Detachment of ligands from nanoparticle surface under flow and endothelial cell contact: Assessment using microfluidic devices"

    Article Title: Detachment of ligands from nanoparticle surface under flow and endothelial cell contact: Assessment using microfluidic devices

    Journal: Bioengineering & Translational Medicine

    doi: 10.1002/btm2.10089

    Changes in ZP on the surface of the nanocrystalline pellet measured post flow for (A) LCs with a base fibronection coating, (B) LCs seeded with endothelial cells, (C) MNs with a base fibronectin coating, and (D) MNs seeded with endothelial cells. Paired t ‐test, n = 3 for all conditions. *** p
    Figure Legend Snippet: Changes in ZP on the surface of the nanocrystalline pellet measured post flow for (A) LCs with a base fibronection coating, (B) LCs seeded with endothelial cells, (C) MNs with a base fibronectin coating, and (D) MNs seeded with endothelial cells. Paired t ‐test, n = 3 for all conditions. *** p

    Techniques Used: Flow Cytometry

    2) Product Images from "Staphylococcal Superantigen-like protein 11 mediates neutrophil adhesion and motility arrest, a unique bacterial toxin action"

    Article Title: Staphylococcal Superantigen-like protein 11 mediates neutrophil adhesion and motility arrest, a unique bacterial toxin action

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-40817-x

    SSL11 stimulates dHL60 cell adhesion. ( A ) 2 μg of purified SSL7, SSL11 and SSL11 3XF were separated by SDS-PAGE and stained with Coomassie Blue. ( B ) dHL60 cells were incubated with 80 nM of SSL7 or SSL11 in fibronectin (FN)-coated plates at 37 °C for 30 min followed by two PBS washes. Representative DIC images were shown. ( C ) dHL60 cells were incubated with SSL7 or SSL11 in FN-coated 96-well plates at 37 °C for 30 min followed by two PBS washes. Adherent cells were quantified by crystal violet staining and shown as adhesion arbitrary unit (AU). ( D ) dHL60 cells were incubated with 80 nM of SSL11 at 37 °C for 30 min and chased in fresh media without SSL11 for another 4 hours in FN-coated plates. Representative DIC images were shown. ( E ) dHL60 cells were treated with SSL11 as described in ( D ) in FN-coated 96 well plates. Adherent cells were quantified by crystal violet staining and shown as adhesion arbitrary unit (AU).
    Figure Legend Snippet: SSL11 stimulates dHL60 cell adhesion. ( A ) 2 μg of purified SSL7, SSL11 and SSL11 3XF were separated by SDS-PAGE and stained with Coomassie Blue. ( B ) dHL60 cells were incubated with 80 nM of SSL7 or SSL11 in fibronectin (FN)-coated plates at 37 °C for 30 min followed by two PBS washes. Representative DIC images were shown. ( C ) dHL60 cells were incubated with SSL7 or SSL11 in FN-coated 96-well plates at 37 °C for 30 min followed by two PBS washes. Adherent cells were quantified by crystal violet staining and shown as adhesion arbitrary unit (AU). ( D ) dHL60 cells were incubated with 80 nM of SSL11 at 37 °C for 30 min and chased in fresh media without SSL11 for another 4 hours in FN-coated plates. Representative DIC images were shown. ( E ) dHL60 cells were treated with SSL11 as described in ( D ) in FN-coated 96 well plates. Adherent cells were quantified by crystal violet staining and shown as adhesion arbitrary unit (AU).

    Techniques Used: Purification, SDS Page, Staining, Incubation

    3) Product Images from "Collective adhesion and displacement of retinal progenitor cells upon extracellular matrix substrates of transplantable biomaterials"

    Article Title: Collective adhesion and displacement of retinal progenitor cells upon extracellular matrix substrates of transplantable biomaterials

    Journal: Journal of Tissue Engineering

    doi: 10.1177/2041731417751286

    Displacement of adhered retinal progenitor cells (RPCs) within the µLane microfluidic system. Images describing attachment of RPC cells and/or neuroclusters within (a) µLane systems whose inner surfaces were functionalized with (b) fibronectin (FN), (c) laminin (LM), (d) hyaluronic acid (HA), and (e) Matrigel (MG). Scale bar = 75 µm.
    Figure Legend Snippet: Displacement of adhered retinal progenitor cells (RPCs) within the µLane microfluidic system. Images describing attachment of RPC cells and/or neuroclusters within (a) µLane systems whose inner surfaces were functionalized with (b) fibronectin (FN), (c) laminin (LM), (d) hyaluronic acid (HA), and (e) Matrigel (MG). Scale bar = 75 µm.

    Techniques Used:

    Differences in gene expression of adhered RPCs. Fold change in regulation of the genes ITGA3, ITGA7, ITGB3, and CD44 that encode (a) integrin α3, integrin α7, integrin β3, and CD44 upon adhesion with fibronectin (FN), laminin (LM), hyaluronic acid (HA), and Matrigel (MG). (b) Schematic representation of up- and down-regulation of encoding genes as measured via the ΔΔCT method.
    Figure Legend Snippet: Differences in gene expression of adhered RPCs. Fold change in regulation of the genes ITGA3, ITGA7, ITGB3, and CD44 that encode (a) integrin α3, integrin α7, integrin β3, and CD44 upon adhesion with fibronectin (FN), laminin (LM), hyaluronic acid (HA), and Matrigel (MG). (b) Schematic representation of up- and down-regulation of encoding genes as measured via the ΔΔCT method.

    Techniques Used: Expressing

    Metrics of individual cell adhesion. The (a) average cell shape index (CSI) of retinal progenitor cells (RPCs) adhered as individual cells upon poly- l -lysine (PLL), fibronectin (FN), laminin (LM), hyaluronic acid (HA), and Matrigel (MG) at cell densities of 10 4 , 10 5 , and10 6 cells/mL. (b) Average values of single cell adhesion density, Π ADH , upon the same biomaterial substrates. Statistical significance within bracketed values is denoted by an asterisk. The “#” symbol indicates a cell monolayer on FN.
    Figure Legend Snippet: Metrics of individual cell adhesion. The (a) average cell shape index (CSI) of retinal progenitor cells (RPCs) adhered as individual cells upon poly- l -lysine (PLL), fibronectin (FN), laminin (LM), hyaluronic acid (HA), and Matrigel (MG) at cell densities of 10 4 , 10 5 , and10 6 cells/mL. (b) Average values of single cell adhesion density, Π ADH , upon the same biomaterial substrates. Statistical significance within bracketed values is denoted by an asterisk. The “#” symbol indicates a cell monolayer on FN.

    Techniques Used:

    Aggregation and adhesion of retinal progenitor cells (RPCs). Images of RPCs at a cell density of 10 5 cells/mL adhered as individual cells and neuroclusters upon surfaces of: (a) poly- l -lysine (PLL), (b) fibronectin (FN), (c) laminin (LM), (d) hyaluronic acid (HA), and (e) Matrigel (MG). Representative cell clusters are outlined per image and arrows point to individually adhered cells. Scale bar = 200 µm. Representative images of RPCs evaluated as (f, g) individually adhered cells, (h) RPCs adhered with close adhesion spacing but not as neuroclusters, and (i) RPCs adhered as part of neuroclusters. Scale bar = 20 µm.
    Figure Legend Snippet: Aggregation and adhesion of retinal progenitor cells (RPCs). Images of RPCs at a cell density of 10 5 cells/mL adhered as individual cells and neuroclusters upon surfaces of: (a) poly- l -lysine (PLL), (b) fibronectin (FN), (c) laminin (LM), (d) hyaluronic acid (HA), and (e) Matrigel (MG). Representative cell clusters are outlined per image and arrows point to individually adhered cells. Scale bar = 200 µm. Representative images of RPCs evaluated as (f, g) individually adhered cells, (h) RPCs adhered with close adhesion spacing but not as neuroclusters, and (i) RPCs adhered as part of neuroclusters. Scale bar = 20 µm.

    Techniques Used:

    Metrics of adhered neuroclusters. The projected surface area of adhered retinal neuroclusters was measured to determine (a) mean cluster size, X CL , upon poly- l -lysine (PLL), fibronectin (FN), laminin (LM), hyaluronic acid (HA), and Matrigel (MG) at cell densities of 10 4 , 10 5 , and10 6 cells/mL. (b) The percentage of adhered cell areas occupied by neuroclusters as measured by the adhesion ratio, R ADH . Statistical significance across and between bracketed values is denoted by an asterisk. The “#” symbol indicates a cell monolayer on FN.
    Figure Legend Snippet: Metrics of adhered neuroclusters. The projected surface area of adhered retinal neuroclusters was measured to determine (a) mean cluster size, X CL , upon poly- l -lysine (PLL), fibronectin (FN), laminin (LM), hyaluronic acid (HA), and Matrigel (MG) at cell densities of 10 4 , 10 5 , and10 6 cells/mL. (b) The percentage of adhered cell areas occupied by neuroclusters as measured by the adhesion ratio, R ADH . Statistical significance across and between bracketed values is denoted by an asterisk. The “#” symbol indicates a cell monolayer on FN.

    Techniques Used:

    4) Product Images from "Collective adhesion and displacement of retinal progenitor cells upon extracellular matrix substrates of transplantable biomaterials"

    Article Title: Collective adhesion and displacement of retinal progenitor cells upon extracellular matrix substrates of transplantable biomaterials

    Journal: Journal of Tissue Engineering

    doi: 10.1177/2041731417751286

    Differences in gene expression of adhered RPCs. Fold change in regulation of the genes ITGA3, ITGA7, ITGB3, and CD44 that encode (a) integrin α3, integrin α7, integrin β3, and CD44 upon adhesion with fibronectin (FN), laminin (LM), hyaluronic acid (HA), and Matrigel (MG). (b) Schematic representation of up- and down-regulation of encoding genes as measured via the ΔΔCT method.
    Figure Legend Snippet: Differences in gene expression of adhered RPCs. Fold change in regulation of the genes ITGA3, ITGA7, ITGB3, and CD44 that encode (a) integrin α3, integrin α7, integrin β3, and CD44 upon adhesion with fibronectin (FN), laminin (LM), hyaluronic acid (HA), and Matrigel (MG). (b) Schematic representation of up- and down-regulation of encoding genes as measured via the ΔΔCT method.

    Techniques Used: Expressing

    5) Product Images from "Heterologously Expressed Staphylococcus aureus Fibronectin-Binding Proteins Are Sufficient for Invasion of Host Cells"

    Article Title: Heterologously Expressed Staphylococcus aureus Fibronectin-Binding Proteins Are Sufficient for Invasion of Host Cells

    Journal: Infection and Immunity

    doi:

    FnBPs expressed in S. carnosus ( S. carn .) specifically bind fibronectin. A Western ligand blot shows cell wall-associated proteins of S. carnosus transformants (strain TM300) expressing FnBPs. FnBPs were detected by a sandwich technique using fibronectin and then anti-N-terminal fibronectin antibodies as described in Materials and Methods. As a control, S. aureus ( S. aur .) strains DU5883(pFNBA4) and DU5883(pFNBB4), expressing the respective FnBPs, were processed and run in parallel. Strain DU5883 did not yield any signal (data not shown). WT, wild type; cl, clone.
    Figure Legend Snippet: FnBPs expressed in S. carnosus ( S. carn .) specifically bind fibronectin. A Western ligand blot shows cell wall-associated proteins of S. carnosus transformants (strain TM300) expressing FnBPs. FnBPs were detected by a sandwich technique using fibronectin and then anti-N-terminal fibronectin antibodies as described in Materials and Methods. As a control, S. aureus ( S. aur .) strains DU5883(pFNBA4) and DU5883(pFNBB4), expressing the respective FnBPs, were processed and run in parallel. Strain DU5883 did not yield any signal (data not shown). WT, wild type; cl, clone.

    Techniques Used: Western Blot, Expressing

    S. carnosus clones expressing FnBPs dose dependently bind to immobilized and soluble fibronectin. (A) Adherence of S. carnosus strain TM300 transformants to immobilized fibronectin (Fn). PMMA coverslips were precoated with 0.5 mg of gelatin per ml in order to optimize fibronectin adsorption (see Materials and Methods). Results are means and SEMs for four independent experiments run in duplicate. (B) Binding of soluble FITC-labeled fibronectin (Fn-FITC) (5 μg/ml) by S. carnosus strain TM300 transformants. Results are means and SEMs for four independent experiments run in duplicate.
    Figure Legend Snippet: S. carnosus clones expressing FnBPs dose dependently bind to immobilized and soluble fibronectin. (A) Adherence of S. carnosus strain TM300 transformants to immobilized fibronectin (Fn). PMMA coverslips were precoated with 0.5 mg of gelatin per ml in order to optimize fibronectin adsorption (see Materials and Methods). Results are means and SEMs for four independent experiments run in duplicate. (B) Binding of soluble FITC-labeled fibronectin (Fn-FITC) (5 μg/ml) by S. carnosus strain TM300 transformants. Results are means and SEMs for four independent experiments run in duplicate.

    Techniques Used: Clone Assay, Expressing, Adsorption, Binding Assay, Labeling

    Related Articles

    Cell Culture:

    Article Title: Potential Mechanism of Dermal Wound Treatment With Preparations From the Skin Gel of Arabian Gulf Catfish: A Unique Furan Fatty Acid (F6) and Cholesta-3,5-Diene (S5) Recruit Neutrophils and Fibroblasts to Promote Wound Healing
    Article Snippet: .. Effect of Ft on the Production of Collagen-I and Fibronectin by Human Dermal FibroblastsAll cell culture products were obtained from GIBCO Life Technologies (Burlington, Ont., Canada). ..

    Article Title: Dextran induces differentiation of circulating endothelial progenitor cells
    Article Snippet: This indicates that ex vivo expanded EPCs keep approximately half of the characteristics of freshly isolated EPCs and have an endothelial phenotype. .. 3 × 104 /cm2 ex vivo expanded EPCs were applied on culture dishes coated with 100 μg/mL solution of human fibronectin (GIBCO, Grand island, NY), and cultured in M199 medium (GIBCO) with 5% fetal bovine serum (FBS; JRH), and EGM2 (VEGF, fibroblast growth factor‐2, epidermal growth factor, insulin‐like growth factor‐1, and ascorbic acid; Clonetics) at 37°C under 5% CO2 atmosphere for 7 days. .. Adhesion assay 2 × 104 EPCs under a dextran‐free condition and exposed to 5% and 10% dextran for 24 h were seeded onto a human fibronectin‐coated 96‐well culture dish in M199 medium with 1% FBS.

    Incubation:

    Article Title: Detachment of ligands from nanoparticle surface under flow and endothelial cell contact: Assessment using microfluidic devices
    Article Snippet: .. MNs and LCs were coated with 100 µg/ml human fibronectin (Thermo Fisher), subjected to 5 PSI N2 (laboratory grade) for 15 min, and incubated in a humidified incubator with 5% CO2 at 37°C in preparation for seeding with EA.hy926 cells. .. Once seeded, EA.hy926 cells were allowed to incubate for 4 hr to attach to the channels of the MNs before changing the media using a syringe pump (KD Scientific Inc.).

    Expressing:

    Article Title: Site-specific epitope insertion into recombinant proteins using the MAP tag system
    Article Snippet: .. Pull-down assays The expression construct for Fn10-Fc contained DNA segments coding for residues 1,417–1,509 of human fibronectin followed by Tobacco Etch Virus protease cleavage sequence and human IgG1 hinge-Fc and was made in pcDNA3.1 (Thermo Fisher Scientific)-based vector containing a bovine prolactin signal sequence. .. Placements of the MAP tag sequence at sites shown in were conducted by extension polymerase chain reaction (PCR).

    Construct:

    Article Title: Site-specific epitope insertion into recombinant proteins using the MAP tag system
    Article Snippet: .. Pull-down assays The expression construct for Fn10-Fc contained DNA segments coding for residues 1,417–1,509 of human fibronectin followed by Tobacco Etch Virus protease cleavage sequence and human IgG1 hinge-Fc and was made in pcDNA3.1 (Thermo Fisher Scientific)-based vector containing a bovine prolactin signal sequence. .. Placements of the MAP tag sequence at sites shown in were conducted by extension polymerase chain reaction (PCR).

    Sequencing:

    Article Title: Site-specific epitope insertion into recombinant proteins using the MAP tag system
    Article Snippet: .. Pull-down assays The expression construct for Fn10-Fc contained DNA segments coding for residues 1,417–1,509 of human fibronectin followed by Tobacco Etch Virus protease cleavage sequence and human IgG1 hinge-Fc and was made in pcDNA3.1 (Thermo Fisher Scientific)-based vector containing a bovine prolactin signal sequence. .. Placements of the MAP tag sequence at sites shown in were conducted by extension polymerase chain reaction (PCR).

    Plasmid Preparation:

    Article Title: Site-specific epitope insertion into recombinant proteins using the MAP tag system
    Article Snippet: .. Pull-down assays The expression construct for Fn10-Fc contained DNA segments coding for residues 1,417–1,509 of human fibronectin followed by Tobacco Etch Virus protease cleavage sequence and human IgG1 hinge-Fc and was made in pcDNA3.1 (Thermo Fisher Scientific)-based vector containing a bovine prolactin signal sequence. .. Placements of the MAP tag sequence at sites shown in were conducted by extension polymerase chain reaction (PCR).

    Ex Vivo:

    Article Title: Dextran induces differentiation of circulating endothelial progenitor cells
    Article Snippet: This indicates that ex vivo expanded EPCs keep approximately half of the characteristics of freshly isolated EPCs and have an endothelial phenotype. .. 3 × 104 /cm2 ex vivo expanded EPCs were applied on culture dishes coated with 100 μg/mL solution of human fibronectin (GIBCO, Grand island, NY), and cultured in M199 medium (GIBCO) with 5% fetal bovine serum (FBS; JRH), and EGM2 (VEGF, fibroblast growth factor‐2, epidermal growth factor, insulin‐like growth factor‐1, and ascorbic acid; Clonetics) at 37°C under 5% CO2 atmosphere for 7 days. .. Adhesion assay 2 × 104 EPCs under a dextran‐free condition and exposed to 5% and 10% dextran for 24 h were seeded onto a human fibronectin‐coated 96‐well culture dish in M199 medium with 1% FBS.

    Fluorescence:

    Article Title: Antiamoebic Activity of Adenophyllum aurantium (L.) Strother and Its Effect on the Actin Cytoskeleton of Entamoeba histolytica
    Article Snippet: After they were dehydrated in increasing concentrations of ethanol and propylene oxide, the samples were embedded in Polybed epoxy resins and polymerized at 60°C for 24 h. Thin sections (60 nm) were contrasted with uranyl acetate and lead citrate before being examined in a Jeol JEM-1011 electron microscope (Jeol, Ltd., Tokyo, Japan). .. Fluorescence Microscopy Amoebas (2 × 105 ) were adhered to coverslips that had been previously covered with 100 μg of purified human fibronectin for 15 min; they were then treated with Aa EaR (230 μg/ml) for 1 h. Bound cells were fixed with 4% ρ-formaldehyde for 1 h at 37°C and stained with rhodamine phalloidin (1:50; R415, Invitrogen, Carlsbad, CA, USA) for 1 h at 37°C. .. The samples were mounted with Vecta-Shield medium (Vector Laboratories, Peterborough, UK) and observed under a Carl Zeiss LSM 700 confocal microscope (Carl Zeiss, Jena, Germany).

    Microscopy:

    Article Title: Antiamoebic Activity of Adenophyllum aurantium (L.) Strother and Its Effect on the Actin Cytoskeleton of Entamoeba histolytica
    Article Snippet: After they were dehydrated in increasing concentrations of ethanol and propylene oxide, the samples were embedded in Polybed epoxy resins and polymerized at 60°C for 24 h. Thin sections (60 nm) were contrasted with uranyl acetate and lead citrate before being examined in a Jeol JEM-1011 electron microscope (Jeol, Ltd., Tokyo, Japan). .. Fluorescence Microscopy Amoebas (2 × 105 ) were adhered to coverslips that had been previously covered with 100 μg of purified human fibronectin for 15 min; they were then treated with Aa EaR (230 μg/ml) for 1 h. Bound cells were fixed with 4% ρ-formaldehyde for 1 h at 37°C and stained with rhodamine phalloidin (1:50; R415, Invitrogen, Carlsbad, CA, USA) for 1 h at 37°C. .. The samples were mounted with Vecta-Shield medium (Vector Laboratories, Peterborough, UK) and observed under a Carl Zeiss LSM 700 confocal microscope (Carl Zeiss, Jena, Germany).

    Purification:

    Article Title: Antiamoebic Activity of Adenophyllum aurantium (L.) Strother and Its Effect on the Actin Cytoskeleton of Entamoeba histolytica
    Article Snippet: After they were dehydrated in increasing concentrations of ethanol and propylene oxide, the samples were embedded in Polybed epoxy resins and polymerized at 60°C for 24 h. Thin sections (60 nm) were contrasted with uranyl acetate and lead citrate before being examined in a Jeol JEM-1011 electron microscope (Jeol, Ltd., Tokyo, Japan). .. Fluorescence Microscopy Amoebas (2 × 105 ) were adhered to coverslips that had been previously covered with 100 μg of purified human fibronectin for 15 min; they were then treated with Aa EaR (230 μg/ml) for 1 h. Bound cells were fixed with 4% ρ-formaldehyde for 1 h at 37°C and stained with rhodamine phalloidin (1:50; R415, Invitrogen, Carlsbad, CA, USA) for 1 h at 37°C. .. The samples were mounted with Vecta-Shield medium (Vector Laboratories, Peterborough, UK) and observed under a Carl Zeiss LSM 700 confocal microscope (Carl Zeiss, Jena, Germany).

    Staining:

    Article Title: Antiamoebic Activity of Adenophyllum aurantium (L.) Strother and Its Effect on the Actin Cytoskeleton of Entamoeba histolytica
    Article Snippet: After they were dehydrated in increasing concentrations of ethanol and propylene oxide, the samples were embedded in Polybed epoxy resins and polymerized at 60°C for 24 h. Thin sections (60 nm) were contrasted with uranyl acetate and lead citrate before being examined in a Jeol JEM-1011 electron microscope (Jeol, Ltd., Tokyo, Japan). .. Fluorescence Microscopy Amoebas (2 × 105 ) were adhered to coverslips that had been previously covered with 100 μg of purified human fibronectin for 15 min; they were then treated with Aa EaR (230 μg/ml) for 1 h. Bound cells were fixed with 4% ρ-formaldehyde for 1 h at 37°C and stained with rhodamine phalloidin (1:50; R415, Invitrogen, Carlsbad, CA, USA) for 1 h at 37°C. .. The samples were mounted with Vecta-Shield medium (Vector Laboratories, Peterborough, UK) and observed under a Carl Zeiss LSM 700 confocal microscope (Carl Zeiss, Jena, Germany).

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  • 94
    Thermo Fisher human plasma fibronectin
    Viability and cell adhesion of ARPE19 cells was not influenced by resveratrol. The cells were treated with different concentrations of resveratrol for 24 hours after being starved for 24 hours. Cell viability was determined by MTT assay (A). BCECF-labeled cells were treated with DMSO or resveratrol for 30 minutes. They were then seeded and allowed to adhere on plates with precoated <t>fibronectin</t> (fn) (15 µg/ml) at 37°C for 1 hour. Fluorescence was measured using excitation and emission wavelength of 485 and 535 nm, respectively (B). The results are expressed as percentage of control and represent the mean ± standard errors (SE) of four independent experiments.
    Human Plasma Fibronectin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human plasma fibronectin/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human plasma fibronectin - by Bioz Stars, 2021-04
    94/100 stars
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    93
    Thermo Fisher human fibronectin
    Effect of dextran on the culture, adhesion, and proliferation. 3 × 10 4 /cm 2 floating endothelial progenitor cells (EPCs) were cultured in medium with 5% dextran (A‐b and ‐e) and 10% dextran (A‐c and ‐f) or without dextran (A‐a and ‐d) on human <t>fibronectin‐coated</t> dishes. After 4 days (A‐a, ‐b, and ‐c) and 7 days (A‐d, ‐e, and ‐f) EPCs were observed by a phase contrast microscope (×10) (A). Dextran induced differentiation of circulating EPCs toward adhesive EPCs. Floating EPCs exposed to various densities of dextran for 24 h were cultured for 6 h and the adhesive cells were observed by a phase contrast microscope (×10) (B). EPCs exposed to dextran significantly increased adhesion. The number of adhesive cells per high‐power field (HPF) was counted (C). N = 3. Floating EPCs exposed to various density of dextran for 24 h were cultured for 24 h and the proliferation activity was measured (D). Dextran increased proliferation. N = 5. Data are means ± SD. ** P
    Human Fibronectin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human fibronectin/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human fibronectin - by Bioz Stars, 2021-04
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    97
    Thermo Fisher human recombinant fibronectin
    Hyaluronic acid production by tumour cells promotes cellular growth a , Quantification of hyaluronic acid (HA) cell surface levels on control (10A-Cont.), transformed (10A-v-Src, 10A-HRAS) and malignant (MCF7, T47D) mammary epithelial cells (MECs). b , Fluorescence micrographs of HA and immuno-labelled paxillin on the v-Src transformed MECs (scale bars, 3 µm). c , Quantification of the number of v-Src-transformed MECs per colony 48 h after plating on soft polyacrylamide gels <t>(fibronectin-conjugated)</t> and treated with vehicle (DMSO), hyaluronic acid synthesis inhibitor 4-methylumbelliferone (+4MU; 0.3 µM), or competitive inhibitor HA oligonucleotides (+Oligo; 12-mer average oligonucleotide size; 100 mg ml −1 ). Results are the mean ± s.e.m with statistical significance is given by * P
    Human Recombinant Fibronectin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human recombinant fibronectin/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human recombinant fibronectin - by Bioz Stars, 2021-04
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    Image Search Results


    Viability and cell adhesion of ARPE19 cells was not influenced by resveratrol. The cells were treated with different concentrations of resveratrol for 24 hours after being starved for 24 hours. Cell viability was determined by MTT assay (A). BCECF-labeled cells were treated with DMSO or resveratrol for 30 minutes. They were then seeded and allowed to adhere on plates with precoated fibronectin (fn) (15 µg/ml) at 37°C for 1 hour. Fluorescence was measured using excitation and emission wavelength of 485 and 535 nm, respectively (B). The results are expressed as percentage of control and represent the mean ± standard errors (SE) of four independent experiments.

    Journal: PLoS ONE

    Article Title: Inhibitory Effects of Resveratrol on PDGF-BB-Induced Retinal Pigment Epithelial Cell Migration via PDGFR?, PI3K/Akt and MAPK pathways

    doi: 10.1371/journal.pone.0056819

    Figure Lengend Snippet: Viability and cell adhesion of ARPE19 cells was not influenced by resveratrol. The cells were treated with different concentrations of resveratrol for 24 hours after being starved for 24 hours. Cell viability was determined by MTT assay (A). BCECF-labeled cells were treated with DMSO or resveratrol for 30 minutes. They were then seeded and allowed to adhere on plates with precoated fibronectin (fn) (15 µg/ml) at 37°C for 1 hour. Fluorescence was measured using excitation and emission wavelength of 485 and 535 nm, respectively (B). The results are expressed as percentage of control and represent the mean ± standard errors (SE) of four independent experiments.

    Article Snippet: Human plasma fibronectin was from Life Technologies Corporation (Carlsbad, CA, USA).

    Techniques: MTT Assay, Labeling, Fluorescence

    Transwell migration assay showed that PDGF-BB-induced ARPE19 cell migration was inhibited by resveratrol. Transwell inserts were coated with fibronectin (0.3 mg). ARPE19 cells (5×10 4 in 200 µl) were seeded in the upper chamber in the absence or presence of resveratrol. The inserts were assembled in the lower chamber, which was filled with 600 µl serum-free medium without PDGF-BB (A) and containing PDGF-BB (20 ng/ml) (B), and preincubated with various concentrations of resveratrol for 30 mininutes at 37°C. After incubating for 5 hours at 37°C, fixation was performed. ARPE19 cells that migrated to the underside of filter membrane were photographed (A, B) and counted by phase contrast light microscope under high power field (magnification, 100×), (C). All experiments were conducted in duplicates and similar results were repeated four times. The results are expressed as percentage of control and represent mean ± standard errors (SE) of the eight experiments. *p

    Journal: PLoS ONE

    Article Title: Inhibitory Effects of Resveratrol on PDGF-BB-Induced Retinal Pigment Epithelial Cell Migration via PDGFR?, PI3K/Akt and MAPK pathways

    doi: 10.1371/journal.pone.0056819

    Figure Lengend Snippet: Transwell migration assay showed that PDGF-BB-induced ARPE19 cell migration was inhibited by resveratrol. Transwell inserts were coated with fibronectin (0.3 mg). ARPE19 cells (5×10 4 in 200 µl) were seeded in the upper chamber in the absence or presence of resveratrol. The inserts were assembled in the lower chamber, which was filled with 600 µl serum-free medium without PDGF-BB (A) and containing PDGF-BB (20 ng/ml) (B), and preincubated with various concentrations of resveratrol for 30 mininutes at 37°C. After incubating for 5 hours at 37°C, fixation was performed. ARPE19 cells that migrated to the underside of filter membrane were photographed (A, B) and counted by phase contrast light microscope under high power field (magnification, 100×), (C). All experiments were conducted in duplicates and similar results were repeated four times. The results are expressed as percentage of control and represent mean ± standard errors (SE) of the eight experiments. *p

    Article Snippet: Human plasma fibronectin was from Life Technologies Corporation (Carlsbad, CA, USA).

    Techniques: Transwell Migration Assay, Migration, Light Microscopy

    Fibronectin collagen peptide force spectroscopy

    Journal:

    Article Title: Structure and Reactivity of Adsorbed Fibronectin Films on Mica

    doi: 10.1529/biophysj.107.109819

    Figure Lengend Snippet: Fibronectin collagen peptide force spectroscopy

    Article Snippet: Protein adsorption Human plasma fibronectin (Invitrogen, Grand Island, NY) was adsorbed onto freshly cleaved mica (Ted Pella, Redding, CA) from solution concentrations ranging from 1 to 100 μg/ml in PBS solution.

    Techniques: Spectroscopy

    Effect of dextran on the culture, adhesion, and proliferation. 3 × 10 4 /cm 2 floating endothelial progenitor cells (EPCs) were cultured in medium with 5% dextran (A‐b and ‐e) and 10% dextran (A‐c and ‐f) or without dextran (A‐a and ‐d) on human fibronectin‐coated dishes. After 4 days (A‐a, ‐b, and ‐c) and 7 days (A‐d, ‐e, and ‐f) EPCs were observed by a phase contrast microscope (×10) (A). Dextran induced differentiation of circulating EPCs toward adhesive EPCs. Floating EPCs exposed to various densities of dextran for 24 h were cultured for 6 h and the adhesive cells were observed by a phase contrast microscope (×10) (B). EPCs exposed to dextran significantly increased adhesion. The number of adhesive cells per high‐power field (HPF) was counted (C). N = 3. Floating EPCs exposed to various density of dextran for 24 h were cultured for 24 h and the proliferation activity was measured (D). Dextran increased proliferation. N = 5. Data are means ± SD. ** P

    Journal: Physiological Reports

    Article Title: Dextran induces differentiation of circulating endothelial progenitor cells

    doi: 10.1002/phy2.261

    Figure Lengend Snippet: Effect of dextran on the culture, adhesion, and proliferation. 3 × 10 4 /cm 2 floating endothelial progenitor cells (EPCs) were cultured in medium with 5% dextran (A‐b and ‐e) and 10% dextran (A‐c and ‐f) or without dextran (A‐a and ‐d) on human fibronectin‐coated dishes. After 4 days (A‐a, ‐b, and ‐c) and 7 days (A‐d, ‐e, and ‐f) EPCs were observed by a phase contrast microscope (×10) (A). Dextran induced differentiation of circulating EPCs toward adhesive EPCs. Floating EPCs exposed to various densities of dextran for 24 h were cultured for 6 h and the adhesive cells were observed by a phase contrast microscope (×10) (B). EPCs exposed to dextran significantly increased adhesion. The number of adhesive cells per high‐power field (HPF) was counted (C). N = 3. Floating EPCs exposed to various density of dextran for 24 h were cultured for 24 h and the proliferation activity was measured (D). Dextran increased proliferation. N = 5. Data are means ± SD. ** P

    Article Snippet: 3 × 104 /cm2 ex vivo expanded EPCs were applied on culture dishes coated with 100 μg/mL solution of human fibronectin (GIBCO, Grand island, NY), and cultured in M199 medium (GIBCO) with 5% fetal bovine serum (FBS; JRH), and EGM2 (VEGF, fibroblast growth factor‐2, epidermal growth factor, insulin‐like growth factor‐1, and ascorbic acid; Clonetics) at 37°C under 5% CO2 atmosphere for 7 days.

    Techniques: Cell Culture, Microscopy, Activity Assay

    Hyaluronic acid production by tumour cells promotes cellular growth a , Quantification of hyaluronic acid (HA) cell surface levels on control (10A-Cont.), transformed (10A-v-Src, 10A-HRAS) and malignant (MCF7, T47D) mammary epithelial cells (MECs). b , Fluorescence micrographs of HA and immuno-labelled paxillin on the v-Src transformed MECs (scale bars, 3 µm). c , Quantification of the number of v-Src-transformed MECs per colony 48 h after plating on soft polyacrylamide gels (fibronectin-conjugated) and treated with vehicle (DMSO), hyaluronic acid synthesis inhibitor 4-methylumbelliferone (+4MU; 0.3 µM), or competitive inhibitor HA oligonucleotides (+Oligo; 12-mer average oligonucleotide size; 100 mg ml −1 ). Results are the mean ± s.e.m with statistical significance is given by * P

    Journal: Nature

    Article Title: The cancer glycocalyx mechanically primes integrin-mediated growth and survival

    doi: 10.1038/nature13535

    Figure Lengend Snippet: Hyaluronic acid production by tumour cells promotes cellular growth a , Quantification of hyaluronic acid (HA) cell surface levels on control (10A-Cont.), transformed (10A-v-Src, 10A-HRAS) and malignant (MCF7, T47D) mammary epithelial cells (MECs). b , Fluorescence micrographs of HA and immuno-labelled paxillin on the v-Src transformed MECs (scale bars, 3 µm). c , Quantification of the number of v-Src-transformed MECs per colony 48 h after plating on soft polyacrylamide gels (fibronectin-conjugated) and treated with vehicle (DMSO), hyaluronic acid synthesis inhibitor 4-methylumbelliferone (+4MU; 0.3 µM), or competitive inhibitor HA oligonucleotides (+Oligo; 12-mer average oligonucleotide size; 100 mg ml −1 ). Results are the mean ± s.e.m with statistical significance is given by * P

    Article Snippet: Human recombinant fibronectin was labelled with N-hydroxysuccinimide Alexa568 (Invitrogen) according to manufacturer’s protocol and dialysed extensively in PBS.

    Techniques: Transformation Assay, Fluorescence

    Tension-dependent integrin activation and focal adhesion assembly in MUC1-expressing cells a , Fluorescence micrographs of fibronectin-crosslinked α5 integrin in control and MUC1-expressing mammary epithelial cells (MECs) treated with solvent alone (DMSO), myosin-II inhibitor (blebbistatin; 50 µM), or Rho kinase inhibitor (Y-27632; 10 µM) for 1 h and detergent-extracted following crosslinking. Only fibronectin-bound integrins under mechanical tension are crosslinked and visualized following detergent extraction (scale bar, 15 µm). b , Fluorescence micrographs showing formation of myosin-independent adhesion complexes in MUC1-expressing MECs. Cells were pre-treated for 1 h and plated for 2 h in 50 µM blebbistatin (scale bar, 10 µm). c , Fluorescence micrographs of paxillin–mCherry and immuno-labelled activated FAK (pY397) in control and MUC1(ΔCT) expressing MECs plated on compliant fibronectin-conjugated hydrogels (E = 140 Pa; scale bar, 3 µm; ROI scale bar, 0.5 µm). d , Western blots showing phosphorylation of paxillin (pY118) in control and MUC1-expressing MECs on compliant substrates (E = 140 Pa) following overnight serum starvation and stimulation with EGF. MUC1-expressing cells treated with a pharmacological inhibitor of focal adhesion kinase (+FAKi) for 1 h before EGF stimulation did not exhibit robust paxillin phosphorylation.

    Journal: Nature

    Article Title: The cancer glycocalyx mechanically primes integrin-mediated growth and survival

    doi: 10.1038/nature13535

    Figure Lengend Snippet: Tension-dependent integrin activation and focal adhesion assembly in MUC1-expressing cells a , Fluorescence micrographs of fibronectin-crosslinked α5 integrin in control and MUC1-expressing mammary epithelial cells (MECs) treated with solvent alone (DMSO), myosin-II inhibitor (blebbistatin; 50 µM), or Rho kinase inhibitor (Y-27632; 10 µM) for 1 h and detergent-extracted following crosslinking. Only fibronectin-bound integrins under mechanical tension are crosslinked and visualized following detergent extraction (scale bar, 15 µm). b , Fluorescence micrographs showing formation of myosin-independent adhesion complexes in MUC1-expressing MECs. Cells were pre-treated for 1 h and plated for 2 h in 50 µM blebbistatin (scale bar, 10 µm). c , Fluorescence micrographs of paxillin–mCherry and immuno-labelled activated FAK (pY397) in control and MUC1(ΔCT) expressing MECs plated on compliant fibronectin-conjugated hydrogels (E = 140 Pa; scale bar, 3 µm; ROI scale bar, 0.5 µm). d , Western blots showing phosphorylation of paxillin (pY118) in control and MUC1-expressing MECs on compliant substrates (E = 140 Pa) following overnight serum starvation and stimulation with EGF. MUC1-expressing cells treated with a pharmacological inhibitor of focal adhesion kinase (+FAKi) for 1 h before EGF stimulation did not exhibit robust paxillin phosphorylation.

    Article Snippet: Human recombinant fibronectin was labelled with N-hydroxysuccinimide Alexa568 (Invitrogen) according to manufacturer’s protocol and dialysed extensively in PBS.

    Techniques: Activation Assay, Expressing, Fluorescence, Western Blot

    MUC1-mediated adhesion formation a , Quantification of the average number of large adhesions, greater than 1 µm 2 , per area of cell in control epithelial cells (Control) and those ectopically expressing ectodomain-truncated MUC1 (+MUC1(ΔTR)), wild-type MUC1 (+MUC1), or cytoplasmic-tail-deleted MUC1 (+MUC1(ΔCT)). Results are the mean ± s.e.m. of three separate experiments. b , Fluorescence micrographs showing immuno-labelled MUC1 and fluorescently labelled fibronectin fibrils in control and MUC1-expressing epithelial cells. Soluble, labelled fibronectin in the growth media was deposited by cells at sites of cell–matrix adhesion. Binding of soluble fibronectin to MUC1 was not detected. Scale bar, 10 µm. c , Time lapse images of MUC1–YFP and vinculin–mCherry, showing the dynamics of adhesion assembly (Vinc.) and MUC1 patterning (MUC1). Scale bar, 1 µm. d , Rate of adhesion measured with single cell force spectroscopy of control (Cont.), α 5 integrin-blocked (anti-α 5 ), and MUC1-expressing cells (+MUC1) to fibronectin-coated surfaces and control cells to BSA-coated surfaces (BSA). Results are the mean ± s.e.m. of at least 15 cell measurements. Statistical significance is given by * P

    Journal: Nature

    Article Title: The cancer glycocalyx mechanically primes integrin-mediated growth and survival

    doi: 10.1038/nature13535

    Figure Lengend Snippet: MUC1-mediated adhesion formation a , Quantification of the average number of large adhesions, greater than 1 µm 2 , per area of cell in control epithelial cells (Control) and those ectopically expressing ectodomain-truncated MUC1 (+MUC1(ΔTR)), wild-type MUC1 (+MUC1), or cytoplasmic-tail-deleted MUC1 (+MUC1(ΔCT)). Results are the mean ± s.e.m. of three separate experiments. b , Fluorescence micrographs showing immuno-labelled MUC1 and fluorescently labelled fibronectin fibrils in control and MUC1-expressing epithelial cells. Soluble, labelled fibronectin in the growth media was deposited by cells at sites of cell–matrix adhesion. Binding of soluble fibronectin to MUC1 was not detected. Scale bar, 10 µm. c , Time lapse images of MUC1–YFP and vinculin–mCherry, showing the dynamics of adhesion assembly (Vinc.) and MUC1 patterning (MUC1). Scale bar, 1 µm. d , Rate of adhesion measured with single cell force spectroscopy of control (Cont.), α 5 integrin-blocked (anti-α 5 ), and MUC1-expressing cells (+MUC1) to fibronectin-coated surfaces and control cells to BSA-coated surfaces (BSA). Results are the mean ± s.e.m. of at least 15 cell measurements. Statistical significance is given by * P

    Article Snippet: Human recombinant fibronectin was labelled with N-hydroxysuccinimide Alexa568 (Invitrogen) according to manufacturer’s protocol and dialysed extensively in PBS.

    Techniques: Expressing, Fluorescence, Binding Assay, Spectroscopy

    Cell proliferation on soft ECM a , Fluorescence micrographs showing DAPI-stained nuclei of control and MUC1(ΔCT)-expressing MECs after 24 h of plating on soft, fibronectin-conjugated hydrogels (E = 140 Pa; scale bar, 250 µm). The majority of cells plated as single cells, indicating that multi-cell colonies that formed at later time points were largely attributed to cell proliferation. b , Quantification of cell proliferation of MUC1(ΔCT)-expressing epithelial cells on soft hydrogels conjugated with bovine serum albumin (BSA) or fibronectin (Fn). Cells plated similarly on BSA– and Fn–hydrogels, but cell proliferation was significantly enhanced on Fn–hydrogels. Results are the mean ± s.e.m with statistical significance given by * P

    Journal: Nature

    Article Title: The cancer glycocalyx mechanically primes integrin-mediated growth and survival

    doi: 10.1038/nature13535

    Figure Lengend Snippet: Cell proliferation on soft ECM a , Fluorescence micrographs showing DAPI-stained nuclei of control and MUC1(ΔCT)-expressing MECs after 24 h of plating on soft, fibronectin-conjugated hydrogels (E = 140 Pa; scale bar, 250 µm). The majority of cells plated as single cells, indicating that multi-cell colonies that formed at later time points were largely attributed to cell proliferation. b , Quantification of cell proliferation of MUC1(ΔCT)-expressing epithelial cells on soft hydrogels conjugated with bovine serum albumin (BSA) or fibronectin (Fn). Cells plated similarly on BSA– and Fn–hydrogels, but cell proliferation was significantly enhanced on Fn–hydrogels. Results are the mean ± s.e.m with statistical significance given by * P

    Article Snippet: Human recombinant fibronectin was labelled with N-hydroxysuccinimide Alexa568 (Invitrogen) according to manufacturer’s protocol and dialysed extensively in PBS.

    Techniques: Fluorescence, Staining, Expressing