Journal: bioRxiv

Article Title: Tomographic volumetric bioprinting of 3D pancreatic cancer models

doi: 10.1101/2023.01.23.525142

Figure Lengend Snippet: a . Pancreatic cancer typically occurs within the functional unit of exocrine pancreas, composed by epithelial cells (acinar and ductal cells) surrounded by pancreatic stellate cells (PSCs). b. Schematic representation of pancreatic intraepithelial neoplasia (PanIN) progression inspired from Liot et al. . Particular focus is made on PSCs activation which triggers the desmoplastic response. c. In tomographic volumetric bioprinting, a gelatin metacryloyl (GelMA) 5% solution in cell medium containing the photoinitiator lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) 0.16 mg mL -1 and 0.5 million fibroblasts (HFF1) mL -1 is poured into glass vials and printed. A cell-laden construct that mimics the functional unit of exocrine pancreas is obtained. d . Photograph of a bioprinted construct (immersed in water) with the cavity filled with a glycerol-based blue dye (for visualization purpose only). e . Human Pancreatic Ductal Epithelial (HPDE) cells, healthy or cancerous, can be injected into fibroblast-laden bioprinted constructs, where they will attach and coat the inner surface of the duct with time. f. To recapitulate fibroblast-associated inflammation or activation, the interaction between HPDE cells stably expressing the KRAS oncogene (HPDE-KRAS) and the surrounding fibroblasts is monitored by measuring the expression of αSMA versus actin in the cytoskeletons of the latter. (Figure drawn using Biorender.com ).

Article Snippet: Human foreskin fibroblasts (HFF1) cells were purchased from ATCC® and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) without phenol red, supplemented with 1% Penicillin-Streptomycin (Gibco), 2% L-glutamine (Gibco) and 15% FBS (Gibco).

Techniques: Functional Assay, Activation Assay, Construct, Injection, Stable Transfection, Expressing

Journal: Pharmaceuticals

Article Title: Tannic Acid and Ethyl Gallate Potentialize Paclitaxel Effect on Microtubule Dynamics in Hep3B Cells

doi: 10.3390/ph16111579

Figure Lengend Snippet: Effect of EG, TA, and PTX on Hep3B cell viability. ( A ) Effect of different concentrations of EG and TA on Hep3B cell viability. ( B ) Effect of different concentrations of PTX alone and in combination with 50 μM EG or 50 μM TA on Hep3B cell viability. ( C ) Effect of different concentrations of EG and TA on HFF-1 cell viability. ( D ) Effect on cell viability of different concentrations of PTX alone and in combination with 50 μM EG or 50 μM TA on HFF-1 cells. Absorbance values are expressed relative to drug-free culture cells (arbitrary value of 100). Results are the mean ± SD of at least three independent experiment repetitions.

Article Snippet: Hep3B (Hepato-Cellular Carcinoma) cells and HFF-1 (Human Foreskin Fibroblasts) cells obtained from ATCC (American Type Culture Collection, Manassas, VA, USA), were cultured in DMEM medium (Invitrogen, Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (SFB, Invitrogen) and 2 mM glutamine, and all cultures were incubated at 37 °C in a 5% CO 2 atmosphere.

Techniques:

Journal: Scientific Reports

Article Title: The study of tryptophol containing emulgel on fungal reduction and skin irritation

doi: 10.1038/s41598-023-46121-z

Figure Lengend Snippet: Cell viability ( a ) and cytotoxicity ( b ) of TOH-treated human foreskin fibroblasts (HFF- 1) cells at 37 °C as determined by MTT, Mitogreen, LDH, and dual AO/EB staining assays. Percentages of cell viability and cytotoxicity were shown as mean ± SD. *P < 0.05 and ****P < 0.0001 indicate significant differences compared with the control group (concentration 0). TOH Tryptophol, VOZ voriconazole.

Article Snippet: Human foreskin fibroblasts (HFF-1) cell line (ATCC #SCRC-1041™) was used to investigate cell viability and cytotoxicity after TOH treatments.

Techniques: Staining, Concentration Assay

Journal: Pharmaceutics

Article Title: Development of Imeglimin Electrospun Nanofibers as a Potential Buccal Antidiabetic Therapeutic Approach

doi: 10.3390/pharmaceutics15041208

Figure Lengend Snippet: Cell viability of different doses of imeglimin upon 24 h HFF-1 cells exposure. The data demonstrated that imeglimin is safe at ≤250 µg/mL. Results are presented as mean ± SD (n = 3).

Article Snippet: It measured the cellular metabolic activity of human fibroblast HFF-1 cells (ATCC- SCRC-1401) following a 24 h exposure to the tested drug.

Techniques:

Journal: Pharmaceutics

Article Title: Design and Evaluation of Pegylated Large 3D Pore Ferrisilicate as a Potential Insulin Protein Therapy to Treat Diabetic Mellitus

doi: 10.3390/pharmaceutics15020593

Figure Lengend Snippet: Cytotoxicity of Insulin/Ferrisilicate/PEG nanoformulations against Human foreskin fibroblast (HFF-1) cells. The cells were treated with Insulin/Ferrislicate/PEG, Insulin/10 wt% Fe/KIT-6/PEG, Ferrisilicate/PEG, 10 wt% FeKIT-6/PEG and insulin for ( a ) 24, ( b ) 48 and ( c ) 72 h. Error bars ± SD. (ns) non-significant p value, * p < 0.05; *** p < 0.001; **** p < 0.0001 as Insulin/Ferrisilicate/PEG versus Insulin/10 wt% Fe/KIT-6/PEG and insulin using two-way ANOVA with Dunnett’s multiple comparison test. The cytotoxicity assay was performed in three independent experiments.

Article Snippet: Human foreskin fibroblast (HFF-1) cells were obtained as (SCRC-1041TM, ATCC, Manassas, VA, USA) and maintained in DMEM supplied with 10% fetal bovine serum, 1% L-glutamine and 1% penicillin-streptomycin (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) in a humidified 5% CO 2 incubator (Galaxy ® 170S, Eppendorf, Stevenage, UK) at 37 °C.

Techniques: Cytotoxicity Assay