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Agilent technologies human epidermal growth factor receptor egfr
High <t>EGFR</t> expression is a strongly associated with high ND. (A) Microscopic analysis of cell membrane EGFR <t>immunohistochemical</t> staining. EGFR expression was graded using a 3-point scale, where 1+ = light staining of more than 10% of the specimens, 2+ = moderate staining of more than 10% and less than or equal to 30% of the specimens, and 3+ = strong staining of more than 30% of the specimens. (B) Statistical analysis of EGFR expression using Student's t -test. EGFR 2+/3+ expression group included more patients with high ND than the EGFR 1+ group. The most suitable ND cutoff level was deemed ND of 35%. (c) Kaplan–Meier curves indicate that EGFR 2+/3+ expression was significantly associated with poor outcome in patients with 13th JGCA stage II/III disease ( P = 0.039). (D) ND ≥35 was significantly associated with poor outcome ( P = 0.0012).
Human Epidermal Growth Factor Receptor Egfr, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Identification of EGFR expression status association with metastatic lymph node density (ND) by expression microarray analysis of advanced gastric cancer"

Article Title: Identification of EGFR expression status association with metastatic lymph node density (ND) by expression microarray analysis of advanced gastric cancer

Journal: Cancer Medicine

doi: 10.1002/cam4.311

High EGFR expression is a strongly associated with high ND. (A) Microscopic analysis of cell membrane EGFR immunohistochemical staining. EGFR expression was graded using a 3-point scale, where 1+ = light staining of more than 10% of the specimens, 2+ = moderate staining of more than 10% and less than or equal to 30% of the specimens, and 3+ = strong staining of more than 30% of the specimens. (B) Statistical analysis of EGFR expression using Student's t -test. EGFR 2+/3+ expression group included more patients with high ND than the EGFR 1+ group. The most suitable ND cutoff level was deemed ND of 35%. (c) Kaplan–Meier curves indicate that EGFR 2+/3+ expression was significantly associated with poor outcome in patients with 13th JGCA stage II/III disease ( P = 0.039). (D) ND ≥35 was significantly associated with poor outcome ( P = 0.0012).
Figure Legend Snippet: High EGFR expression is a strongly associated with high ND. (A) Microscopic analysis of cell membrane EGFR immunohistochemical staining. EGFR expression was graded using a 3-point scale, where 1+ = light staining of more than 10% of the specimens, 2+ = moderate staining of more than 10% and less than or equal to 30% of the specimens, and 3+ = strong staining of more than 30% of the specimens. (B) Statistical analysis of EGFR expression using Student's t -test. EGFR 2+/3+ expression group included more patients with high ND than the EGFR 1+ group. The most suitable ND cutoff level was deemed ND of 35%. (c) Kaplan–Meier curves indicate that EGFR 2+/3+ expression was significantly associated with poor outcome in patients with 13th JGCA stage II/III disease ( P = 0.039). (D) ND ≥35 was significantly associated with poor outcome ( P = 0.0012).

Techniques Used: Expressing, Immunohistochemistry, Staining

Related Articles

Blocking Assay:

Article Title: Synaptic Regulator α-Synuclein in Dopaminergic Fibers Is Essentially Required for the Maintenance of Subependymal Neural Stem Cells
Article Snippet: For BrdU immunodetection, sections were first treated with 2N HCl for 17 min at 37°C followed by several phosphate buffer rinses. .. After blocking, sections were incubated in rabbit antibodies to α-SYN (1:1200, AbD Serotec), Ki67 (1:150, Abcam), β-catenin (1:100, Cell Signaling Technology), TH (1:600, Pel-Freez), β-III-tubulin (1:175, Sigma-Aldrich), S100β (1:100, Dako), or epidermal growth factor receptor (EGFR) (1:100, Cell Signaling Technology), mouse antibodies to S100β (1:500, Sigma-Aldrich), β-III-tubulin (1:200, Covance), Ki67 (1:50, Novocastra), α-SYN (1:1200, BD Biosciences), Ascl1 (1:150, BD Biosciences), Neu-N (1:100, Millipore), TH (1:500, Sigma-Aldrich), or human α-SYN (1:1350, Abcam, ab27766), rat antibodies to BrdU (1:800, Abcam), goat antibodies to Sox2 (1:150, R & D Systems), γ-tubulin (1:150, Santa Cruz Biotechnology), and chicken antibodies to GFAP (1:800, Millipore), alone or in different combinations for 24–48 h at 4°C. .. After several washes, the sections were incubated for 1 h at room temperature with appropriate fluorescently labeled secondary antibodies: Alexa-555-conjugated goat anti-mouse, Alexa-488-goat anti-rabbit and anti-mouse, Alexa-647-goat anti-rabbit, and Alexa-488-donkey anti-chicken at 1:500 (Invitrogen), or Cy3-donkey anti-chicken and Cy3-goat anti-mouse (1:1000, Jackson ImmunoResearch Laboratories).

Incubation:

Article Title: Synaptic Regulator α-Synuclein in Dopaminergic Fibers Is Essentially Required for the Maintenance of Subependymal Neural Stem Cells
Article Snippet: For BrdU immunodetection, sections were first treated with 2N HCl for 17 min at 37°C followed by several phosphate buffer rinses. .. After blocking, sections were incubated in rabbit antibodies to α-SYN (1:1200, AbD Serotec), Ki67 (1:150, Abcam), β-catenin (1:100, Cell Signaling Technology), TH (1:600, Pel-Freez), β-III-tubulin (1:175, Sigma-Aldrich), S100β (1:100, Dako), or epidermal growth factor receptor (EGFR) (1:100, Cell Signaling Technology), mouse antibodies to S100β (1:500, Sigma-Aldrich), β-III-tubulin (1:200, Covance), Ki67 (1:50, Novocastra), α-SYN (1:1200, BD Biosciences), Ascl1 (1:150, BD Biosciences), Neu-N (1:100, Millipore), TH (1:500, Sigma-Aldrich), or human α-SYN (1:1350, Abcam, ab27766), rat antibodies to BrdU (1:800, Abcam), goat antibodies to Sox2 (1:150, R & D Systems), γ-tubulin (1:150, Santa Cruz Biotechnology), and chicken antibodies to GFAP (1:800, Millipore), alone or in different combinations for 24–48 h at 4°C. .. After several washes, the sections were incubated for 1 h at room temperature with appropriate fluorescently labeled secondary antibodies: Alexa-555-conjugated goat anti-mouse, Alexa-488-goat anti-rabbit and anti-mouse, Alexa-647-goat anti-rabbit, and Alexa-488-donkey anti-chicken at 1:500 (Invitrogen), or Cy3-donkey anti-chicken and Cy3-goat anti-mouse (1:1000, Jackson ImmunoResearch Laboratories).

Immunostaining:

Article Title: Gene Expression Profile of Glioblastoma Peritumoral Tissue: An Ex Vivo Study
Article Snippet: Subsequently, slides were incubated with a HRP/Fab polymer conjugate (SuperPicTure Polymer Detection Kit, Invitrogen, Camarillo, CA, USA). .. The immunostaining for Epidermal Growth Factor Receptor (EGFR) and CD99 was performed using the monoclonal antibodies anti- human EGFR (1∶100; Clone E30; Dako) and CD99 (1∶100; clone 12E7; Dako) on an autostainer (Dako Autostainer Plus Link, Dako). .. The antigen retrieval was performed using pronase digestion (ProTaqs® Pronase Digest, Germany) and En Vision™ Flex Target Retrieval Solution High pH (Dako) for EGFR and CD99, respectively.

Immunohistochemistry:

Article Title: Identification of EGFR expression status association with metastatic lymph node density (ND) by expression microarray analysis of advanced gastric cancer
Article Snippet: .. Immunohistochemical staining of the EGFR The primary antibodies used for immunohistochemical (IHC) assays were the mouse monoclonal antibodies against the human epidermal growth factor receptor (EGFR) that are included in the EGFR pharmDx kit (Dako-Japan, Tokyo, Japan). .. Routine formalin-fixed, paraffin-embedded tissue samples obtained from resected gastric cancer specimens were analyzed.

Article Title: Expansion of Multipotent Stem Cells from the Adult Human Brain
Article Snippet: .. Antibodies used for Immunohistochemical Labeling Rabbit anti-Gal C, 1/200 (Sigma); rabbit anti-GFAP, 1/200 (DakoCytomation, Denmark); rabbit anti-Integrin β1, 1/200 (Abcam); rabbit anti-Musashi, 1/200 (Abcam); rabbit anti-Dopamine transporter, 1/200 (Sigma); goat anti-Oct 4, 1/200 (R & D Systems); goat anti-human Sox2, 1/200 (R & D Systems); mouse anti-Tyrosine Hydroxylase, 1/50 (Sigma); mouse anti-O4, 1/400 (Millipore); mouse anti-S100, 1/200 (Abcam); mouse anti-Map2, 1/200 (Millipore); mouse anti-Neurofilament 200, 1/200 (Sigma); mouse anti-Nestin, 1/200 (Millipore); mouse anti-Prolyl-4-hydroxylase, 1/200 (BioSite, Sweden); mouse anti-Ki67, 1/200 (DakoCytomation, Denmark); mouse anti-EGFr (Epidermal growth factor receptor), 1/200 (Abcam); mouse anti-sarcomeric αActin, 1/200 (Sigma); mouse anti-cardiac Troponin I, 1/200 (Millipore); rabbit anti-smooth αActin, 1/200; and goat anti-Green Fluorescent Protein (GFP), 1/300 (Santa Cruz). ..

Article Title: Pattern of recurrence of early breast cancer is different according to intrinsic subtype and proliferation index
Article Snippet: Tissue microarrays were constructed, and the cases were represented in triplicate with samples from 0.6-mm cores. .. Immunohistochemical staining was performed for ER (clone SP1; Master Diagnóstica, Granada, Spain), progesterone receptor (PR) (clone Y85; Master Diagnóstica), human epidermal growth factor receptor 2 (HER2) (HercepTest; Dako, Glostrup, Denmark), Ki-67 (clone SP6; Master Diagnóstica), epidermal growth factor receptor (EGFR) (clone EP38Y; Master Diagnóstica) and cytokeratin types 5 and 6 (CK5/6) (clone D5/16B4; Master Diagnóstica). .. Staining and interpretation of ER, PR, HER2, Ki-67, EGFR and CK5/6 were performed as previously described [ - ].

Article Title: Biology of Oestrogen-Receptor Positive Primary Breast Cancer in Older Women with Utilisation of Core Needle Biopsy Samples and Correlation with Clinical Outcome
Article Snippet: .. Measurement of Panel of BiomarkersImmunohistochemical (IHC) staining of 17 biomarkers was performed using StreptAvidin Biotin Complex and EnVision methods (DakoCytomation) [ ]. .. The biomarkers measured were: ER, progesterone receptor (PgR), Ki67, Epidermal Growth Factor Receptor (EGFR), Human Epidermal Growth Factor Receptor (HER)-2, HER3, HER4, p53, cytokeratins CK5/6 and CK7/8, Mucin (MUC)1, liver kinase B1 (LKB1), Breast Cancer Associated gene (BRCA)-1, B-Cell Lymphoma (BCL)-2, phosphate and tensin homolog (PTEN), vascular endothelial growth factor (VEGF), and Amplified in breast cancer 1 (AIB1).

Staining:

Article Title: Identification of EGFR expression status association with metastatic lymph node density (ND) by expression microarray analysis of advanced gastric cancer
Article Snippet: .. Immunohistochemical staining of the EGFR The primary antibodies used for immunohistochemical (IHC) assays were the mouse monoclonal antibodies against the human epidermal growth factor receptor (EGFR) that are included in the EGFR pharmDx kit (Dako-Japan, Tokyo, Japan). .. Routine formalin-fixed, paraffin-embedded tissue samples obtained from resected gastric cancer specimens were analyzed.

Article Title: Pattern of recurrence of early breast cancer is different according to intrinsic subtype and proliferation index
Article Snippet: Tissue microarrays were constructed, and the cases were represented in triplicate with samples from 0.6-mm cores. .. Immunohistochemical staining was performed for ER (clone SP1; Master Diagnóstica, Granada, Spain), progesterone receptor (PR) (clone Y85; Master Diagnóstica), human epidermal growth factor receptor 2 (HER2) (HercepTest; Dako, Glostrup, Denmark), Ki-67 (clone SP6; Master Diagnóstica), epidermal growth factor receptor (EGFR) (clone EP38Y; Master Diagnóstica) and cytokeratin types 5 and 6 (CK5/6) (clone D5/16B4; Master Diagnóstica). .. Staining and interpretation of ER, PR, HER2, Ki-67, EGFR and CK5/6 were performed as previously described [ - ].

Article Title: Biology of Oestrogen-Receptor Positive Primary Breast Cancer in Older Women with Utilisation of Core Needle Biopsy Samples and Correlation with Clinical Outcome
Article Snippet: .. Measurement of Panel of BiomarkersImmunohistochemical (IHC) staining of 17 biomarkers was performed using StreptAvidin Biotin Complex and EnVision methods (DakoCytomation) [ ]. .. The biomarkers measured were: ER, progesterone receptor (PgR), Ki67, Epidermal Growth Factor Receptor (EGFR), Human Epidermal Growth Factor Receptor (HER)-2, HER3, HER4, p53, cytokeratins CK5/6 and CK7/8, Mucin (MUC)1, liver kinase B1 (LKB1), Breast Cancer Associated gene (BRCA)-1, B-Cell Lymphoma (BCL)-2, phosphate and tensin homolog (PTEN), vascular endothelial growth factor (VEGF), and Amplified in breast cancer 1 (AIB1).

Labeling:

Article Title: Expansion of Multipotent Stem Cells from the Adult Human Brain
Article Snippet: .. Antibodies used for Immunohistochemical Labeling Rabbit anti-Gal C, 1/200 (Sigma); rabbit anti-GFAP, 1/200 (DakoCytomation, Denmark); rabbit anti-Integrin β1, 1/200 (Abcam); rabbit anti-Musashi, 1/200 (Abcam); rabbit anti-Dopamine transporter, 1/200 (Sigma); goat anti-Oct 4, 1/200 (R & D Systems); goat anti-human Sox2, 1/200 (R & D Systems); mouse anti-Tyrosine Hydroxylase, 1/50 (Sigma); mouse anti-O4, 1/400 (Millipore); mouse anti-S100, 1/200 (Abcam); mouse anti-Map2, 1/200 (Millipore); mouse anti-Neurofilament 200, 1/200 (Sigma); mouse anti-Nestin, 1/200 (Millipore); mouse anti-Prolyl-4-hydroxylase, 1/200 (BioSite, Sweden); mouse anti-Ki67, 1/200 (DakoCytomation, Denmark); mouse anti-EGFr (Epidermal growth factor receptor), 1/200 (Abcam); mouse anti-sarcomeric αActin, 1/200 (Sigma); mouse anti-cardiac Troponin I, 1/200 (Millipore); rabbit anti-smooth αActin, 1/200; and goat anti-Green Fluorescent Protein (GFP), 1/300 (Santa Cruz). ..

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    Agilent technologies human epidermal growth factor receptor egfr
    High <t>EGFR</t> expression is a strongly associated with high ND. (A) Microscopic analysis of cell membrane EGFR <t>immunohistochemical</t> staining. EGFR expression was graded using a 3-point scale, where 1+ = light staining of more than 10% of the specimens, 2+ = moderate staining of more than 10% and less than or equal to 30% of the specimens, and 3+ = strong staining of more than 30% of the specimens. (B) Statistical analysis of EGFR expression using Student's t -test. EGFR 2+/3+ expression group included more patients with high ND than the EGFR 1+ group. The most suitable ND cutoff level was deemed ND of 35%. (c) Kaplan–Meier curves indicate that EGFR 2+/3+ expression was significantly associated with poor outcome in patients with 13th JGCA stage II/III disease ( P = 0.039). (D) ND ≥35 was significantly associated with poor outcome ( P = 0.0012).
    Human Epidermal Growth Factor Receptor Egfr, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human epidermal growth factor receptor egfr/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human epidermal growth factor receptor egfr - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    86
    Agilent technologies monoclonal mouse anti human epidermal growth factor receptor egfr
    High <t>EGFR</t> expression is a strongly associated with high ND. (A) Microscopic analysis of cell membrane EGFR <t>immunohistochemical</t> staining. EGFR expression was graded using a 3-point scale, where 1+ = light staining of more than 10% of the specimens, 2+ = moderate staining of more than 10% and less than or equal to 30% of the specimens, and 3+ = strong staining of more than 30% of the specimens. (B) Statistical analysis of EGFR expression using Student's t -test. EGFR 2+/3+ expression group included more patients with high ND than the EGFR 1+ group. The most suitable ND cutoff level was deemed ND of 35%. (c) Kaplan–Meier curves indicate that EGFR 2+/3+ expression was significantly associated with poor outcome in patients with 13th JGCA stage II/III disease ( P = 0.039). (D) ND ≥35 was significantly associated with poor outcome ( P = 0.0012).
    Monoclonal Mouse Anti Human Epidermal Growth Factor Receptor Egfr, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal mouse anti human epidermal growth factor receptor egfr/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal mouse anti human epidermal growth factor receptor egfr - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    86
    Agilent technologies human epidermal growth factor receptor 1
    High <t>EGFR</t> expression is a strongly associated with high ND. (A) Microscopic analysis of cell membrane EGFR <t>immunohistochemical</t> staining. EGFR expression was graded using a 3-point scale, where 1+ = light staining of more than 10% of the specimens, 2+ = moderate staining of more than 10% and less than or equal to 30% of the specimens, and 3+ = strong staining of more than 30% of the specimens. (B) Statistical analysis of EGFR expression using Student's t -test. EGFR 2+/3+ expression group included more patients with high ND than the EGFR 1+ group. The most suitable ND cutoff level was deemed ND of 35%. (c) Kaplan–Meier curves indicate that EGFR 2+/3+ expression was significantly associated with poor outcome in patients with 13th JGCA stage II/III disease ( P = 0.039). (D) ND ≥35 was significantly associated with poor outcome ( P = 0.0012).
    Human Epidermal Growth Factor Receptor 1, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human epidermal growth factor receptor 1/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human epidermal growth factor receptor 1 - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

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    High EGFR expression is a strongly associated with high ND. (A) Microscopic analysis of cell membrane EGFR immunohistochemical staining. EGFR expression was graded using a 3-point scale, where 1+ = light staining of more than 10% of the specimens, 2+ = moderate staining of more than 10% and less than or equal to 30% of the specimens, and 3+ = strong staining of more than 30% of the specimens. (B) Statistical analysis of EGFR expression using Student's t -test. EGFR 2+/3+ expression group included more patients with high ND than the EGFR 1+ group. The most suitable ND cutoff level was deemed ND of 35%. (c) Kaplan–Meier curves indicate that EGFR 2+/3+ expression was significantly associated with poor outcome in patients with 13th JGCA stage II/III disease ( P = 0.039). (D) ND ≥35 was significantly associated with poor outcome ( P = 0.0012).

    Journal: Cancer Medicine

    Article Title: Identification of EGFR expression status association with metastatic lymph node density (ND) by expression microarray analysis of advanced gastric cancer

    doi: 10.1002/cam4.311

    Figure Lengend Snippet: High EGFR expression is a strongly associated with high ND. (A) Microscopic analysis of cell membrane EGFR immunohistochemical staining. EGFR expression was graded using a 3-point scale, where 1+ = light staining of more than 10% of the specimens, 2+ = moderate staining of more than 10% and less than or equal to 30% of the specimens, and 3+ = strong staining of more than 30% of the specimens. (B) Statistical analysis of EGFR expression using Student's t -test. EGFR 2+/3+ expression group included more patients with high ND than the EGFR 1+ group. The most suitable ND cutoff level was deemed ND of 35%. (c) Kaplan–Meier curves indicate that EGFR 2+/3+ expression was significantly associated with poor outcome in patients with 13th JGCA stage II/III disease ( P = 0.039). (D) ND ≥35 was significantly associated with poor outcome ( P = 0.0012).

    Article Snippet: Immunohistochemical staining of the EGFR The primary antibodies used for immunohistochemical (IHC) assays were the mouse monoclonal antibodies against the human epidermal growth factor receptor (EGFR) that are included in the EGFR pharmDx kit (Dako-Japan, Tokyo, Japan).

    Techniques: Expressing, Immunohistochemistry, Staining