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human endometrial stromal cells  (ATCC)


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    ATCC human endometrial stromal cells
    Human Endometrial Stromal Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human endometrial stromal cells/product/ATCC
    Average 95 stars, based on 1 article reviews
    human endometrial stromal cells - by Bioz Stars, 2025-06
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    Suppression of the effects of invasion, migration, and EMT in EC cells by knockdown of COL12A1. (a-c) Assessment of the transfection efficiency of COL12A1 by RT-qPCR and Western blot. (d and e) CCK-8 assay evaluation of the viability of EC cells. (f-h) Cell migration assay detection of the migratory capacity of EC cells (Magnification 40×). (i-k) Transwell assay measurement of the invasion capabilities of EC cells (Magnification 40×). (l-n) Western blot analysis of the protein expression levels of N-cadherin, Vimentin, and E-cadherin in EC cells. ✶ P < 0.05 compared with si-NC group. Scale bar = 100 μm. Statistical analysis of significance using one-way ANOVA or Student’s t -test. COL12A1: Collagen type XII alpha 1 chain, EMT: Epithelial-mesenchymal transformation, EC: Endometrial cancer, ANOVA: Analysis of variance, RT-qPCR: Quantitative real-time polymerase chain reaction.

    Journal: CytoJournal

    Article Title: Inhibitory effect on endometrial cancer: Collagen type XII α1 chain

    doi: 10.25259/Cytojournal_236_2024

    Figure Lengend Snippet: Suppression of the effects of invasion, migration, and EMT in EC cells by knockdown of COL12A1. (a-c) Assessment of the transfection efficiency of COL12A1 by RT-qPCR and Western blot. (d and e) CCK-8 assay evaluation of the viability of EC cells. (f-h) Cell migration assay detection of the migratory capacity of EC cells (Magnification 40×). (i-k) Transwell assay measurement of the invasion capabilities of EC cells (Magnification 40×). (l-n) Western blot analysis of the protein expression levels of N-cadherin, Vimentin, and E-cadherin in EC cells. ✶ P < 0.05 compared with si-NC group. Scale bar = 100 μm. Statistical analysis of significance using one-way ANOVA or Student’s t -test. COL12A1: Collagen type XII alpha 1 chain, EMT: Epithelial-mesenchymal transformation, EC: Endometrial cancer, ANOVA: Analysis of variance, RT-qPCR: Quantitative real-time polymerase chain reaction.

    Article Snippet: Human endometrial mesenchymal stromal cells (HESCs) and EC cells Ishikawa (cat. SC0202), AN3CA (cat. SC0199), HEC-1A (cat.SC0204), and RL-95 (cat.SC0200) were cultured in high-glucose Dulbecco’s Modified Eagle Medium (DMEM, cat. C11960500BT, Gibco, Grand Island, NY, USA).

    Techniques: Migration, Knockdown, Transfection, Quantitative RT-PCR, Western Blot, CCK-8 Assay, Cell Migration Assay, Transwell Assay, Expressing, Transformation Assay, Real-time Polymerase Chain Reaction

    Effect of COL12A1 on EC cell invasion, cell migration, and EMT by inducing macrophage M2 polarization. (a) Assessment of EC cell viability through CCK-8 assay. (b-d) Evaluation of the invasive and migratory abilities of EC cells by Transwell assay (Magnification 40×). (e-h) Western blot analysis of the protein expression levels of N-cadherin, Vimentin, and E-cadherin in EC cells. ✶ ✶ P < 0.01 and ✶ ✶ ✶ P < 0.001. Scale bar = 100 μm. OD: Optical density. Statistical analysis of significance using one-way ANOVA. COL12A1: Collagen type XII alpha 1 chain, EMT: Epithelial-mesenchymal transformation, EC: Endometrial cancer, ANOVA: Analysis of variance.

    Journal: CytoJournal

    Article Title: Inhibitory effect on endometrial cancer: Collagen type XII α1 chain

    doi: 10.25259/Cytojournal_236_2024

    Figure Lengend Snippet: Effect of COL12A1 on EC cell invasion, cell migration, and EMT by inducing macrophage M2 polarization. (a) Assessment of EC cell viability through CCK-8 assay. (b-d) Evaluation of the invasive and migratory abilities of EC cells by Transwell assay (Magnification 40×). (e-h) Western blot analysis of the protein expression levels of N-cadherin, Vimentin, and E-cadherin in EC cells. ✶ ✶ P < 0.01 and ✶ ✶ ✶ P < 0.001. Scale bar = 100 μm. OD: Optical density. Statistical analysis of significance using one-way ANOVA. COL12A1: Collagen type XII alpha 1 chain, EMT: Epithelial-mesenchymal transformation, EC: Endometrial cancer, ANOVA: Analysis of variance.

    Article Snippet: Human endometrial mesenchymal stromal cells (HESCs) and EC cells Ishikawa (cat. SC0202), AN3CA (cat. SC0199), HEC-1A (cat.SC0204), and RL-95 (cat.SC0200) were cultured in high-glucose Dulbecco’s Modified Eagle Medium (DMEM, cat. C11960500BT, Gibco, Grand Island, NY, USA).

    Techniques: Migration, CCK-8 Assay, Transwell Assay, Western Blot, Expressing, Transformation Assay