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human embryonic kidney 293 cell line hek293t  (ATCC)


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    ATCC human embryonic kidney 293 cell line hek293t
    Human Embryonic Kidney 293 Cell Line Hek293t, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human embryonic kidney 293 cell line hek293t/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human embryonic kidney 293 cell line hek293t - by Bioz Stars, 2024-11
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    ATCC human embryonic kidney 293 cell line hek293t
    Human Embryonic Kidney 293 Cell Line Hek293t, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    ATCC cell culture human embryonic kidney 293 cell line hek293t
    Cell Culture Human Embryonic Kidney 293 Cell Line Hek293t, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell culture human embryonic kidney 293 cell line hek293t/product/ATCC
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    ATCC human embryonic kidney hek 293 cell line
    Human Embryonic Kidney Hek 293 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human embryonic kidney hek 293 cell line/product/ATCC
    Average 86 stars, based on 1 article reviews
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    human embryonic kidney hek 293 cell line - by Bioz Stars, 2024-11
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    ATCC human embryonic kidney 293 hek293 cell line
    a , b The gels illustrate the resulting epichaperome formation and the phosphorylation status of HSP90 in lysed MDA-MB-468 epichaperome-positive cancer cells treated with CK2 inhibitors. Detection of epichaperome components was done through SDS–PAGE (total protein levels) and native PAGE followed by immunoblotting. a The effect of CX4945 treatment, while b depicts the effect of CIBG-300 treatment. Vehicle-treated cells serve as controls. CK2α levels are shown to verify that inhibitor treatment effects are independent of changes in CK2α expression levels. c Same as in ( a , b ) for CK2α knockdown using dose-dependent siRNAs in MDA-MB-468 cells. CK2α levels, knockdown efficiency control. d The indicated CK2 constructs were used to transfect <t>HEK293</t> cells. CK2α, catalytic subunit; CK2β, regulatory subunit; kinase-dead mutant CK2 K68M α. HA tag and CK2α levels, transfection efficacy control. a – d Gel images are representative of three independent experiments. Source data are provided as a Source data file. e Schematic summary. CK2’s phosphorylation activity directly influences the stability and assembly of epichaperomes. These findings confirm the functional role of HSP90 phosphorylation at these specific serine residues in epichaperome formation and posit CK2 as a likely physiological candidate behind epichaperome formation.
    Human Embryonic Kidney 293 Hek293 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human embryonic kidney 293 hek293 cell line/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human embryonic kidney 293 hek293 cell line - by Bioz Stars, 2024-11
    86/100 stars
      Buy from Supplier

    86
    ATCC human embryonic kidney hek 293 cell lines
    a , b The gels illustrate the resulting epichaperome formation and the phosphorylation status of HSP90 in lysed MDA-MB-468 epichaperome-positive cancer cells treated with CK2 inhibitors. Detection of epichaperome components was done through SDS–PAGE (total protein levels) and native PAGE followed by immunoblotting. a The effect of CX4945 treatment, while b depicts the effect of CIBG-300 treatment. Vehicle-treated cells serve as controls. CK2α levels are shown to verify that inhibitor treatment effects are independent of changes in CK2α expression levels. c Same as in ( a , b ) for CK2α knockdown using dose-dependent siRNAs in MDA-MB-468 cells. CK2α levels, knockdown efficiency control. d The indicated CK2 constructs were used to transfect <t>HEK293</t> cells. CK2α, catalytic subunit; CK2β, regulatory subunit; kinase-dead mutant CK2 K68M α. HA tag and CK2α levels, transfection efficacy control. a – d Gel images are representative of three independent experiments. Source data are provided as a Source data file. e Schematic summary. CK2’s phosphorylation activity directly influences the stability and assembly of epichaperomes. These findings confirm the functional role of HSP90 phosphorylation at these specific serine residues in epichaperome formation and posit CK2 as a likely physiological candidate behind epichaperome formation.
    Human Embryonic Kidney Hek 293 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human embryonic kidney hek 293 cell lines/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human embryonic kidney hek 293 cell lines - by Bioz Stars, 2024-11
    86/100 stars
      Buy from Supplier

    86
    ATCC human embryonic kidney cell line hek 293
    a , b The gels illustrate the resulting epichaperome formation and the phosphorylation status of HSP90 in lysed MDA-MB-468 epichaperome-positive cancer cells treated with CK2 inhibitors. Detection of epichaperome components was done through SDS–PAGE (total protein levels) and native PAGE followed by immunoblotting. a The effect of CX4945 treatment, while b depicts the effect of CIBG-300 treatment. Vehicle-treated cells serve as controls. CK2α levels are shown to verify that inhibitor treatment effects are independent of changes in CK2α expression levels. c Same as in ( a , b ) for CK2α knockdown using dose-dependent siRNAs in MDA-MB-468 cells. CK2α levels, knockdown efficiency control. d The indicated CK2 constructs were used to transfect <t>HEK293</t> cells. CK2α, catalytic subunit; CK2β, regulatory subunit; kinase-dead mutant CK2 K68M α. HA tag and CK2α levels, transfection efficacy control. a – d Gel images are representative of three independent experiments. Source data are provided as a Source data file. e Schematic summary. CK2’s phosphorylation activity directly influences the stability and assembly of epichaperomes. These findings confirm the functional role of HSP90 phosphorylation at these specific serine residues in epichaperome formation and posit CK2 as a likely physiological candidate behind epichaperome formation.
    Human Embryonic Kidney Cell Line Hek 293, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human embryonic kidney cell line hek 293/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human embryonic kidney cell line hek 293 - by Bioz Stars, 2024-11
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    86
    Thermo Fisher human embryonic kidney cell line 293 t
    a , b The gels illustrate the resulting epichaperome formation and the phosphorylation status of HSP90 in lysed MDA-MB-468 epichaperome-positive cancer cells treated with CK2 inhibitors. Detection of epichaperome components was done through SDS–PAGE (total protein levels) and native PAGE followed by immunoblotting. a The effect of CX4945 treatment, while b depicts the effect of CIBG-300 treatment. Vehicle-treated cells serve as controls. CK2α levels are shown to verify that inhibitor treatment effects are independent of changes in CK2α expression levels. c Same as in ( a , b ) for CK2α knockdown using dose-dependent siRNAs in MDA-MB-468 cells. CK2α levels, knockdown efficiency control. d The indicated CK2 constructs were used to transfect <t>HEK293</t> cells. CK2α, catalytic subunit; CK2β, regulatory subunit; kinase-dead mutant CK2 K68M α. HA tag and CK2α levels, transfection efficacy control. a – d Gel images are representative of three independent experiments. Source data are provided as a Source data file. e Schematic summary. CK2’s phosphorylation activity directly influences the stability and assembly of epichaperomes. These findings confirm the functional role of HSP90 phosphorylation at these specific serine residues in epichaperome formation and posit CK2 as a likely physiological candidate behind epichaperome formation.
    Human Embryonic Kidney Cell Line 293 T, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human embryonic kidney cell line 293 t/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
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    human embryonic kidney cell line 293 t - by Bioz Stars, 2024-11
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    86
    ATCC cell culture human embryonic kidney 293 hek293 cell line
    a , b The gels illustrate the resulting epichaperome formation and the phosphorylation status of HSP90 in lysed MDA-MB-468 epichaperome-positive cancer cells treated with CK2 inhibitors. Detection of epichaperome components was done through SDS–PAGE (total protein levels) and native PAGE followed by immunoblotting. a The effect of CX4945 treatment, while b depicts the effect of CIBG-300 treatment. Vehicle-treated cells serve as controls. CK2α levels are shown to verify that inhibitor treatment effects are independent of changes in CK2α expression levels. c Same as in ( a , b ) for CK2α knockdown using dose-dependent siRNAs in MDA-MB-468 cells. CK2α levels, knockdown efficiency control. d The indicated CK2 constructs were used to transfect <t>HEK293</t> cells. CK2α, catalytic subunit; CK2β, regulatory subunit; kinase-dead mutant CK2 K68M α. HA tag and CK2α levels, transfection efficacy control. a – d Gel images are representative of three independent experiments. Source data are provided as a Source data file. e Schematic summary. CK2’s phosphorylation activity directly influences the stability and assembly of epichaperomes. These findings confirm the functional role of HSP90 phosphorylation at these specific serine residues in epichaperome formation and posit CK2 as a likely physiological candidate behind epichaperome formation.
    Cell Culture Human Embryonic Kidney 293 Hek293 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell culture human embryonic kidney 293 hek293 cell line/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cell culture human embryonic kidney 293 hek293 cell line - by Bioz Stars, 2024-11
    86/100 stars
      Buy from Supplier

    Image Search Results


    a , b The gels illustrate the resulting epichaperome formation and the phosphorylation status of HSP90 in lysed MDA-MB-468 epichaperome-positive cancer cells treated with CK2 inhibitors. Detection of epichaperome components was done through SDS–PAGE (total protein levels) and native PAGE followed by immunoblotting. a The effect of CX4945 treatment, while b depicts the effect of CIBG-300 treatment. Vehicle-treated cells serve as controls. CK2α levels are shown to verify that inhibitor treatment effects are independent of changes in CK2α expression levels. c Same as in ( a , b ) for CK2α knockdown using dose-dependent siRNAs in MDA-MB-468 cells. CK2α levels, knockdown efficiency control. d The indicated CK2 constructs were used to transfect HEK293 cells. CK2α, catalytic subunit; CK2β, regulatory subunit; kinase-dead mutant CK2 K68M α. HA tag and CK2α levels, transfection efficacy control. a – d Gel images are representative of three independent experiments. Source data are provided as a Source data file. e Schematic summary. CK2’s phosphorylation activity directly influences the stability and assembly of epichaperomes. These findings confirm the functional role of HSP90 phosphorylation at these specific serine residues in epichaperome formation and posit CK2 as a likely physiological candidate behind epichaperome formation.

    Journal: Nature Communications

    Article Title: Phosphorylation-driven epichaperome assembly is a regulator of cellular adaptability and proliferation

    doi: 10.1038/s41467-024-53178-5

    Figure Lengend Snippet: a , b The gels illustrate the resulting epichaperome formation and the phosphorylation status of HSP90 in lysed MDA-MB-468 epichaperome-positive cancer cells treated with CK2 inhibitors. Detection of epichaperome components was done through SDS–PAGE (total protein levels) and native PAGE followed by immunoblotting. a The effect of CX4945 treatment, while b depicts the effect of CIBG-300 treatment. Vehicle-treated cells serve as controls. CK2α levels are shown to verify that inhibitor treatment effects are independent of changes in CK2α expression levels. c Same as in ( a , b ) for CK2α knockdown using dose-dependent siRNAs in MDA-MB-468 cells. CK2α levels, knockdown efficiency control. d The indicated CK2 constructs were used to transfect HEK293 cells. CK2α, catalytic subunit; CK2β, regulatory subunit; kinase-dead mutant CK2 K68M α. HA tag and CK2α levels, transfection efficacy control. a – d Gel images are representative of three independent experiments. Source data are provided as a Source data file. e Schematic summary. CK2’s phosphorylation activity directly influences the stability and assembly of epichaperomes. These findings confirm the functional role of HSP90 phosphorylation at these specific serine residues in epichaperome formation and posit CK2 as a likely physiological candidate behind epichaperome formation.

    Article Snippet: The MDA-MB-468 (female, breast cancer cell line, HTB-132, RRID: CVCL_0419), ASPC1 (female, pancreatic cancer cell line, CRL-1682, RRID: CVCL_0152), NCI-H1975 (female, non-small cell lung cancer cell line, CRL-5908, RRID: CVCL_1511), Daudi (male, B lymphoblast cell line, CCL-213, RRID: CVCL_0008), MRC5 (male, lung fibroblast cell line, CCL-171, RRID:CVCL_0440), CCD-18Co (female, colon fibroblast cell line, CRL-1459, RRID: CVCL_2379) and the Human Embryonic Kidney 293 (HEK293) cell line (CRL-1573, RRID: CVCL_0045), of female origin as determined by sequencing, were purchased from ATCC.

    Techniques: SDS Page, Clear Native PAGE, Western Blot, Expressing, Knockdown, Control, Construct, Mutagenesis, Transfection, Activity Assay, Functional Assay