Journal: Cell & Bioscience

Article Title: Aberrant methylation of Serpine1 mediates lung injury in neonatal mice prenatally exposed to intrauterine inflammation

doi: 10.1186/s13578-022-00901-8

Figure Lengend Snippet: Demethylation promoted Rela but inhibited Hdac2 binding to the Serpine1 promoter. A–C Serpine1 expression analysed using RT-qPCR and western blot in Rela-, Hif1a-, and Rest-overexpressing MLE-12 cells. D Transcriptional activity of Rela, Hif1a, and Rest at the Serpine1 promoter analysed using luciferase assay in MLE-12 cells. E The binding ability of Rela and Hdac2 to the biotin-labelled Serpine1 promoter probe analysed via DNA pull-down assay. F–H Serpine1 expression analysed using RT-qPCR and western blot in Hdac2-knockdown MLE-12 cells. I Transcriptional activity of Rela, Hif1a, and Rest at the methylated-Serpine1 promoter analysed using luciferase assay in HEK293T cells. J Transcriptional activity of Rela at the Serpine1 promoter analysed using luciferase assay in Hdac2-knockdown MLE-12 cells. K The enrichment of TFs at the Serpine1 promoter region determined using anti-Rela and anti-Hdac2 antibodies via ChIP-qPCR in 5-Aza-CdR-treated MLE-12 cells. Data show mean ± SEM; Data was analysed using unpaired t -tests

Article Snippet: MLE-12, A549, BEAS-2B and human epithelial kidney 293 T (HEK293T) cell lines were purchased from ATCC.

Techniques: Binding Assay, Expressing, Quantitative RT-PCR, Western Blot, Activity Assay, Luciferase, Pull Down Assay, Methylation

Journal: Cell reports

Article Title: A bidirectional competitive interaction between circHomer1 and Homer1b within the orbitofrontal cortex regulates reversal learning

doi: 10.1016/j.celrep.2021.110282

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: HEK293 embryonic kidney human cell line experiments HEK293 epithelial embryonic kidney cells were purchased from ATCC CRL-1573™.

Techniques: Derivative Assay, Recombinant, Isolation, SYBR Green Assay, Expressing, Sequencing, shRNA, Plasmid Preparation, Software

Journal: Journal of Clinical Medicine

Article Title: GSKIP-Mediated Anchoring Increases Phosphorylation of Tau by PKA but Not by GSK3beta via cAMP/PKA/GSKIP/GSK3/Tau Axis Signaling in Cerebrospinal Fluid and iPS Cells in Alzheimer Disease

doi: 10.3390/jcm8101751

Figure Lengend Snippet: Tau interacts with glycogen synthase kinase 3β (GSK3β), GSK3β interaction protein (GSKIP), and protein kinase A (PKA) in HEK293 cells. pEGFP-C1-Tau, pET32a-HA-GSKIP (L130P), or pET32a-HA-GSKIP (V41/L45P) transfected cells were collected, and total lysates were subjected to IP using anti-GFP antibody. The resulting precipitates were then analyzed through immunoblotting with anti-GFP, GSK3β, HA, and PKA antibodies.

Article Snippet: The human neuroblastoma SH-SY5Y and human renal epithelial (HEK293) cell lines (American Type Culture Collection, Manassas, VA, USA) were used for these experiments.

Techniques: Transfection, Western Blot