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hek 293t human embryonic kidney cells  (ATCC)


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    ATCC hek 293t human embryonic kidney cells
    Hek 293t Human Embryonic Kidney Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hek 293t human embryonic kidney cells/product/ATCC
    Average 86 stars, based on 1 article reviews
    hek 293t human embryonic kidney cells - by Bioz Stars, 2025-02
    86/100 stars

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    The SOX5 expression and protein stability analysis. A The transfection in Neuro-2a cells was assessed using flow cytometry. The wild-type group exhibited a transfection efficiency of 32.5%, which was higher than the 24.4% observed in the mutant group, and the difference was not significant. Cells expressing GFP are shown in blue, while those lacking GFP are depicted in green. B The mRNA expression levels of SOX5 in <t>293T</t> cells and Neuro-2a cells. There was a significant increase in the variant mRNA compared with wild-type (**** p < 0.0001). C – E Western blot analysis was conducted to evaluate the expression of SOX5 and cyclin A in Neuro-2a cells. The mutant SOX5 exhibited elevated expression levels compared to the wild-type in Neuro-2a cells (**** p < 0.0001). The cyclin A expression in the mutant SOX5 was expressed at a lower level than in the wild-type in Neuro-2a cells (*** p < 0.001). F – G Western blot analysis of SOX5 in 293T cells. The SOX5 p.Thr74Met variant was expressed at a higher level than the wild-type in 293T cells (****p < 0.0001). H – I Western blot analysis of the SOX5 protein stability in Neuro-2a cells. The SOX5 protein stability was also increased compared with the wild-type protein in Neuro-2a cells (*** p < 0.001, **** p < 0.0001). J – K Western blot analysis of the SOX5 protein stability in 293T cells. The SOX5 protein stability was also increased compared with the wild-type protein in 293T cells (****p < 0.0001)
    Human Embryonic Kidney Hek 293t Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human embryonic kidney hek 293t cells/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
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    ATCC human embryonic kidney 293t cells
    The SOX5 expression and protein stability analysis. A The transfection in Neuro-2a cells was assessed using flow cytometry. The wild-type group exhibited a transfection efficiency of 32.5%, which was higher than the 24.4% observed in the mutant group, and the difference was not significant. Cells expressing GFP are shown in blue, while those lacking GFP are depicted in green. B The mRNA expression levels of SOX5 in <t>293T</t> cells and Neuro-2a cells. There was a significant increase in the variant mRNA compared with wild-type (**** p < 0.0001). C – E Western blot analysis was conducted to evaluate the expression of SOX5 and cyclin A in Neuro-2a cells. The mutant SOX5 exhibited elevated expression levels compared to the wild-type in Neuro-2a cells (**** p < 0.0001). The cyclin A expression in the mutant SOX5 was expressed at a lower level than in the wild-type in Neuro-2a cells (*** p < 0.001). F – G Western blot analysis of SOX5 in 293T cells. The SOX5 p.Thr74Met variant was expressed at a higher level than the wild-type in 293T cells (****p < 0.0001). H – I Western blot analysis of the SOX5 protein stability in Neuro-2a cells. The SOX5 protein stability was also increased compared with the wild-type protein in Neuro-2a cells (*** p < 0.001, **** p < 0.0001). J – K Western blot analysis of the SOX5 protein stability in 293T cells. The SOX5 protein stability was also increased compared with the wild-type protein in 293T cells (****p < 0.0001)
    Human Embryonic Kidney 293t Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human embryonic kidney 293t cells/product/ATCC
    Average 86 stars, based on 1 article reviews
    human embryonic kidney 293t cells - by Bioz Stars, 2025-02
    86/100 stars
      Buy from Supplier

    86
    ATCC human embryonic kidney hek 293t cell line
    The SOX5 expression and protein stability analysis. A The transfection in Neuro-2a cells was assessed using flow cytometry. The wild-type group exhibited a transfection efficiency of 32.5%, which was higher than the 24.4% observed in the mutant group, and the difference was not significant. Cells expressing GFP are shown in blue, while those lacking GFP are depicted in green. B The mRNA expression levels of SOX5 in <t>293T</t> cells and Neuro-2a cells. There was a significant increase in the variant mRNA compared with wild-type (**** p < 0.0001). C – E Western blot analysis was conducted to evaluate the expression of SOX5 and cyclin A in Neuro-2a cells. The mutant SOX5 exhibited elevated expression levels compared to the wild-type in Neuro-2a cells (**** p < 0.0001). The cyclin A expression in the mutant SOX5 was expressed at a lower level than in the wild-type in Neuro-2a cells (*** p < 0.001). F – G Western blot analysis of SOX5 in 293T cells. The SOX5 p.Thr74Met variant was expressed at a higher level than the wild-type in 293T cells (****p < 0.0001). H – I Western blot analysis of the SOX5 protein stability in Neuro-2a cells. The SOX5 protein stability was also increased compared with the wild-type protein in Neuro-2a cells (*** p < 0.001, **** p < 0.0001). J – K Western blot analysis of the SOX5 protein stability in 293T cells. The SOX5 protein stability was also increased compared with the wild-type protein in 293T cells (****p < 0.0001)
    Human Embryonic Kidney Hek 293t Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human embryonic kidney hek 293t cell line/product/ATCC
    Average 86 stars, based on 1 article reviews
    human embryonic kidney hek 293t cell line - by Bioz Stars, 2025-02
    86/100 stars
      Buy from Supplier

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    The SOX5 expression and protein stability analysis. A The transfection in Neuro-2a cells was assessed using flow cytometry. The wild-type group exhibited a transfection efficiency of 32.5%, which was higher than the 24.4% observed in the mutant group, and the difference was not significant. Cells expressing GFP are shown in blue, while those lacking GFP are depicted in green. B The mRNA expression levels of SOX5 in 293T cells and Neuro-2a cells. There was a significant increase in the variant mRNA compared with wild-type (**** p < 0.0001). C – E Western blot analysis was conducted to evaluate the expression of SOX5 and cyclin A in Neuro-2a cells. The mutant SOX5 exhibited elevated expression levels compared to the wild-type in Neuro-2a cells (**** p < 0.0001). The cyclin A expression in the mutant SOX5 was expressed at a lower level than in the wild-type in Neuro-2a cells (*** p < 0.001). F – G Western blot analysis of SOX5 in 293T cells. The SOX5 p.Thr74Met variant was expressed at a higher level than the wild-type in 293T cells (****p < 0.0001). H – I Western blot analysis of the SOX5 protein stability in Neuro-2a cells. The SOX5 protein stability was also increased compared with the wild-type protein in Neuro-2a cells (*** p < 0.001, **** p < 0.0001). J – K Western blot analysis of the SOX5 protein stability in 293T cells. The SOX5 protein stability was also increased compared with the wild-type protein in 293T cells (****p < 0.0001)

    Journal: Orphanet Journal of Rare Diseases

    Article Title: A missense variant in the SOX5 gene (c.221C > T) is associated with intellectual disability

    doi: 10.1186/s13023-025-03548-z

    Figure Lengend Snippet: The SOX5 expression and protein stability analysis. A The transfection in Neuro-2a cells was assessed using flow cytometry. The wild-type group exhibited a transfection efficiency of 32.5%, which was higher than the 24.4% observed in the mutant group, and the difference was not significant. Cells expressing GFP are shown in blue, while those lacking GFP are depicted in green. B The mRNA expression levels of SOX5 in 293T cells and Neuro-2a cells. There was a significant increase in the variant mRNA compared with wild-type (**** p < 0.0001). C – E Western blot analysis was conducted to evaluate the expression of SOX5 and cyclin A in Neuro-2a cells. The mutant SOX5 exhibited elevated expression levels compared to the wild-type in Neuro-2a cells (**** p < 0.0001). The cyclin A expression in the mutant SOX5 was expressed at a lower level than in the wild-type in Neuro-2a cells (*** p < 0.001). F – G Western blot analysis of SOX5 in 293T cells. The SOX5 p.Thr74Met variant was expressed at a higher level than the wild-type in 293T cells (****p < 0.0001). H – I Western blot analysis of the SOX5 protein stability in Neuro-2a cells. The SOX5 protein stability was also increased compared with the wild-type protein in Neuro-2a cells (*** p < 0.001, **** p < 0.0001). J – K Western blot analysis of the SOX5 protein stability in 293T cells. The SOX5 protein stability was also increased compared with the wild-type protein in 293T cells (****p < 0.0001)

    Article Snippet: Human embryonic kidney (HEK) 293T cells and Neuro-2a cells, at 70% confluence, were transfected with 1 μg of the recombinant plasmids containing wild-type ( pEGFP-N1-SOX5-WT ) or mutant SOX5 c.221C > T genes ( pEGFP-N1-SOX5-MUT ), using Lipofectamine™ 3000 transfection reagent (Invitrogen, NY, USA).

    Techniques: Expressing, Transfection, Flow Cytometry, Mutagenesis, Variant Assay, Western Blot

    Cell cycle analysis and expression analysis of SOX5 target gene. A Localization of wild and mutant in 293T cells and Neuro-2a cells. Wild-type and mutant SOX5 were mainly localized in the nucleus in 293T cells and Neuro-2a cells. Confocal images of EGFP (green), DAPI nuclear staining (blue), and merged signals. B – C Flow cytometry analysis of the cell cycle in Neuro-2a cells: wild-type and mutant. The numbers of S phases in mutant SOX5 were expressed at a lower level than in the wild-type (* p < 0.05). D After transfection of Neuro-2a cells, cell proliferation was detected by CCK-8 assay. E – H The mRNA expression analysis of SOX5 target gene in 293T cells. The mRNA expression levels of ACAN , AXIN2 , SOX9 and PDGFRA were decreased compared with the wild-type (**** p < 0.0001)

    Journal: Orphanet Journal of Rare Diseases

    Article Title: A missense variant in the SOX5 gene (c.221C > T) is associated with intellectual disability

    doi: 10.1186/s13023-025-03548-z

    Figure Lengend Snippet: Cell cycle analysis and expression analysis of SOX5 target gene. A Localization of wild and mutant in 293T cells and Neuro-2a cells. Wild-type and mutant SOX5 were mainly localized in the nucleus in 293T cells and Neuro-2a cells. Confocal images of EGFP (green), DAPI nuclear staining (blue), and merged signals. B – C Flow cytometry analysis of the cell cycle in Neuro-2a cells: wild-type and mutant. The numbers of S phases in mutant SOX5 were expressed at a lower level than in the wild-type (* p < 0.05). D After transfection of Neuro-2a cells, cell proliferation was detected by CCK-8 assay. E – H The mRNA expression analysis of SOX5 target gene in 293T cells. The mRNA expression levels of ACAN , AXIN2 , SOX9 and PDGFRA were decreased compared with the wild-type (**** p < 0.0001)

    Article Snippet: Human embryonic kidney (HEK) 293T cells and Neuro-2a cells, at 70% confluence, were transfected with 1 μg of the recombinant plasmids containing wild-type ( pEGFP-N1-SOX5-WT ) or mutant SOX5 c.221C > T genes ( pEGFP-N1-SOX5-MUT ), using Lipofectamine™ 3000 transfection reagent (Invitrogen, NY, USA).

    Techniques: Cell Cycle Assay, Expressing, Mutagenesis, Staining, Flow Cytometry, Transfection, CCK-8 Assay