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Comparison of SunTag-ePE3 with canonical fused ePE3, Split-ePE3, and MS2-ePE3 at four endogenous loci, including HEK3 locus ( a ), EMX1 locus ( b ), FANCF locus ( c ), and RNF2 locus ( d ) in <t>HEK293T</t> cells. Each locus contains three types of prime editing: nucleotide transversion, insertion, and deletion. Data and error bars indicate the mean and s.d. of three independent biological replicates. Negative controls represent four kinds of PE3 components without pegRNAs. The numbers above the bars represent fold change between different treatment groups.
Human Embryonic Kidney 293 T Hek293t Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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human embryonic kidney 293 t hek293t cells  (ATCC)
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Comparison of SunTag-ePE3 with canonical fused ePE3, Split-ePE3, and MS2-ePE3 at four endogenous loci, including HEK3 locus ( a ), EMX1 locus ( b ), FANCF locus ( c ), and RNF2 locus ( d ) in <t>HEK293T</t> cells. Each locus contains three types of prime editing: nucleotide transversion, insertion, and deletion. Data and error bars indicate the mean and s.d. of three independent biological replicates. Negative controls represent four kinds of PE3 components without pegRNAs. The numbers above the bars represent fold change between different treatment groups.
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Comparison of SunTag-ePE3 with canonical fused ePE3, Split-ePE3, and MS2-ePE3 at four endogenous loci, including HEK3 locus ( a ), EMX1 locus ( b ), FANCF locus ( c ), and RNF2 locus ( d ) in HEK293T cells. Each locus contains three types of prime editing: nucleotide transversion, insertion, and deletion. Data and error bars indicate the mean and s.d. of three independent biological replicates. Negative controls represent four kinds of PE3 components without pegRNAs. The numbers above the bars represent fold change between different treatment groups.

Journal: Communications Biology

Article Title: SunTag-PE: a modular prime editing system enables versatile and efficient genome editing

doi: 10.1038/s42003-025-07893-4

Figure Lengend Snippet: Comparison of SunTag-ePE3 with canonical fused ePE3, Split-ePE3, and MS2-ePE3 at four endogenous loci, including HEK3 locus ( a ), EMX1 locus ( b ), FANCF locus ( c ), and RNF2 locus ( d ) in HEK293T cells. Each locus contains three types of prime editing: nucleotide transversion, insertion, and deletion. Data and error bars indicate the mean and s.d. of three independent biological replicates. Negative controls represent four kinds of PE3 components without pegRNAs. The numbers above the bars represent fold change between different treatment groups.

Article Snippet: Human Embryonic Kidney 293 T (HEK293T) cell line and HeLa cervical cancer cell line were purchased from ATCC.

Techniques: Comparison