human dnase i  (Thermo Fisher)


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    Structured Review

    Thermo Fisher human dnase i
    Enzymatic resistance of phosphorothioate-modified Au-TDNNs. (A, B) Fluorescence time graph depicting terminal-modified Au-TDNN and overall-modified Au-TDNN degradation by DNase I. (C, D) Fluorescence time graph depicting terminal-modified Au-TDNN and overall-modified Au-TDNN degradation by Exo III.
    Human Dnase I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human dnase i/product/Thermo Fisher
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    human dnase i - by Bioz Stars, 2020-04
    93/100 stars

    Images

    1) Product Images from "High-Discrimination Factor Nanosensor Based on Tetrahedral DNA Nanostructures and Gold Nanoparticles for Detection of MiRNA-21 in Live Cells"

    Article Title: High-Discrimination Factor Nanosensor Based on Tetrahedral DNA Nanostructures and Gold Nanoparticles for Detection of MiRNA-21 in Live Cells

    Journal: Theranostics

    doi: 10.7150/thno.23852

    Enzymatic resistance of phosphorothioate-modified Au-TDNNs. (A, B) Fluorescence time graph depicting terminal-modified Au-TDNN and overall-modified Au-TDNN degradation by DNase I. (C, D) Fluorescence time graph depicting terminal-modified Au-TDNN and overall-modified Au-TDNN degradation by Exo III.
    Figure Legend Snippet: Enzymatic resistance of phosphorothioate-modified Au-TDNNs. (A, B) Fluorescence time graph depicting terminal-modified Au-TDNN and overall-modified Au-TDNN degradation by DNase I. (C, D) Fluorescence time graph depicting terminal-modified Au-TDNN and overall-modified Au-TDNN degradation by Exo III.

    Techniques Used: Modification, Fluorescence

    2) Product Images from "Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity"

    Article Title: Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.1621226114

    Expression of enzymes anchored on the yeast surface. ( A ) Genetic constructs for expression of enzymes exposed on the surface of yeast cells. ( B ) Active BChE, EK, and DNase I are displayed on the yeast cell surface. Visualization was performed using antibodies
    Figure Legend Snippet: Expression of enzymes anchored on the yeast surface. ( A ) Genetic constructs for expression of enzymes exposed on the surface of yeast cells. ( B ) Active BChE, EK, and DNase I are displayed on the yeast cell surface. Visualization was performed using antibodies

    Techniques Used: Expressing, Construct

    3) Product Images from "High-Discrimination Factor Nanosensor Based on Tetrahedral DNA Nanostructures and Gold Nanoparticles for Detection of MiRNA-21 in Live Cells"

    Article Title: High-Discrimination Factor Nanosensor Based on Tetrahedral DNA Nanostructures and Gold Nanoparticles for Detection of MiRNA-21 in Live Cells

    Journal: Theranostics

    doi: 10.7150/thno.23852

    Enzymatic resistance of phosphorothioate-modified Au-TDNNs. (A, B) Fluorescence time graph depicting terminal-modified Au-TDNN and overall-modified Au-TDNN degradation by DNase I. (C, D) Fluorescence time graph depicting terminal-modified Au-TDNN and overall-modified Au-TDNN degradation by Exo III.
    Figure Legend Snippet: Enzymatic resistance of phosphorothioate-modified Au-TDNNs. (A, B) Fluorescence time graph depicting terminal-modified Au-TDNN and overall-modified Au-TDNN degradation by DNase I. (C, D) Fluorescence time graph depicting terminal-modified Au-TDNN and overall-modified Au-TDNN degradation by Exo III.

    Techniques Used: Modification, Fluorescence

    Related Articles

    Clone Assay:

    Article Title: Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity
    Article Snippet: Recombinant BChE, human EK, and human DNase I were produced in Pichia pastoris GS115 (Invitrogen) using the modified expression vector pPICZ-mCherry-F2A-HSAss-AfeI / PvuI-HA-SAG1 based on pPICZαA (Invitrogen). .. This vector contained the fluorescent reporter protein mCherry, self-processing F2A peptide, the human serum albumin (HSA) signal sequence, AfeI and PvuI cloning sites, a-agglutinin anchor subunit Aga1p from Saccharomyces cerevisiae , and an HA epitope.

    Fluorescence:

    Article Title: High-Discrimination Factor Nanosensor Based on Tetrahedral DNA Nanostructures and Gold Nanoparticles for Detection of MiRNA-21 in Live Cells
    Article Snippet: Enzymatic resistance of Au-TDNNs Digestion of the two groups of phosphorothioate-protected Au-TDNNs was probed using the Agilent Cary Eclipse to detect generation of nonspecific FAM fluorescence signals. .. For both human DNase I (Thermo Scientific) and exonuclease III (New England Biolabs) digestion, a common concentration of 3 U/mL was used to digest 2 nM Au-TDNNs samples in PBS.

    Polymerase Chain Reaction:

    Article Title: Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity
    Article Snippet: Recombinant BChE, human EK, and human DNase I were produced in Pichia pastoris GS115 (Invitrogen) using the modified expression vector pPICZ-mCherry-F2A-HSAss-AfeI / PvuI-HA-SAG1 based on pPICZαA (Invitrogen). .. The fragments encoding BChE, EK, and DNase I sequences were PCR-amplified from pFUSE PRAD-F2A-BChE , pHENm/ l -HEP/C122S , and p6E-rhDNaseI, which was kindly provided by Pharmsynthez PJSC, and were cloned into vector pPICZ-mCherry-F2A-HSAss-AfeI / PvuI-HA-SAG1 digested with AfeI and PvuI.

    Produced:

    Article Title: Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity
    Article Snippet: .. Recombinant BChE, human EK, and human DNase I were produced in Pichia pastoris GS115 (Invitrogen) using the modified expression vector pPICZ-mCherry-F2A-HSAss-AfeI / PvuI-HA-SAG1 based on pPICZαA (Invitrogen). .. This vector contained the fluorescent reporter protein mCherry, self-processing F2A peptide, the human serum albumin (HSA) signal sequence, AfeI and PvuI cloning sites, a-agglutinin anchor subunit Aga1p from Saccharomyces cerevisiae , and an HA epitope.

    Concentration Assay:

    Article Title: High-Discrimination Factor Nanosensor Based on Tetrahedral DNA Nanostructures and Gold Nanoparticles for Detection of MiRNA-21 in Live Cells
    Article Snippet: .. For both human DNase I (Thermo Scientific) and exonuclease III (New England Biolabs) digestion, a common concentration of 3 U/mL was used to digest 2 nM Au-TDNNs samples in PBS. .. FAM fluorescence signals were monitored and recorded over a period of 3 h at 30 min intervals and at 37 °C.

    Expressing:

    Article Title: Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity
    Article Snippet: .. Recombinant BChE, human EK, and human DNase I were produced in Pichia pastoris GS115 (Invitrogen) using the modified expression vector pPICZ-mCherry-F2A-HSAss-AfeI / PvuI-HA-SAG1 based on pPICZαA (Invitrogen). .. This vector contained the fluorescent reporter protein mCherry, self-processing F2A peptide, the human serum albumin (HSA) signal sequence, AfeI and PvuI cloning sites, a-agglutinin anchor subunit Aga1p from Saccharomyces cerevisiae , and an HA epitope.

    Modification:

    Article Title: Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity
    Article Snippet: .. Recombinant BChE, human EK, and human DNase I were produced in Pichia pastoris GS115 (Invitrogen) using the modified expression vector pPICZ-mCherry-F2A-HSAss-AfeI / PvuI-HA-SAG1 based on pPICZαA (Invitrogen). .. This vector contained the fluorescent reporter protein mCherry, self-processing F2A peptide, the human serum albumin (HSA) signal sequence, AfeI and PvuI cloning sites, a-agglutinin anchor subunit Aga1p from Saccharomyces cerevisiae , and an HA epitope.

    Sequencing:

    Article Title: Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity
    Article Snippet: Recombinant BChE, human EK, and human DNase I were produced in Pichia pastoris GS115 (Invitrogen) using the modified expression vector pPICZ-mCherry-F2A-HSAss-AfeI / PvuI-HA-SAG1 based on pPICZαA (Invitrogen). .. This vector contained the fluorescent reporter protein mCherry, self-processing F2A peptide, the human serum albumin (HSA) signal sequence, AfeI and PvuI cloning sites, a-agglutinin anchor subunit Aga1p from Saccharomyces cerevisiae , and an HA epitope.

    Recombinant:

    Article Title: Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity
    Article Snippet: .. Recombinant BChE, human EK, and human DNase I were produced in Pichia pastoris GS115 (Invitrogen) using the modified expression vector pPICZ-mCherry-F2A-HSAss-AfeI / PvuI-HA-SAG1 based on pPICZαA (Invitrogen). .. This vector contained the fluorescent reporter protein mCherry, self-processing F2A peptide, the human serum albumin (HSA) signal sequence, AfeI and PvuI cloning sites, a-agglutinin anchor subunit Aga1p from Saccharomyces cerevisiae , and an HA epitope.

    Plasmid Preparation:

    Article Title: Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity
    Article Snippet: .. Recombinant BChE, human EK, and human DNase I were produced in Pichia pastoris GS115 (Invitrogen) using the modified expression vector pPICZ-mCherry-F2A-HSAss-AfeI / PvuI-HA-SAG1 based on pPICZαA (Invitrogen). .. This vector contained the fluorescent reporter protein mCherry, self-processing F2A peptide, the human serum albumin (HSA) signal sequence, AfeI and PvuI cloning sites, a-agglutinin anchor subunit Aga1p from Saccharomyces cerevisiae , and an HA epitope.

    Similar Products

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  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Thermo Fisher human dnase i
    Enzymatic resistance of phosphorothioate-modified Au-TDNNs. (A, B) Fluorescence time graph depicting terminal-modified Au-TDNN and overall-modified Au-TDNN degradation by DNase I. (C, D) Fluorescence time graph depicting terminal-modified Au-TDNN and overall-modified Au-TDNN degradation by Exo III.
    Human Dnase I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human dnase i/product/Thermo Fisher
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    human dnase i - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    Image Search Results


    Enzymatic resistance of phosphorothioate-modified Au-TDNNs. (A, B) Fluorescence time graph depicting terminal-modified Au-TDNN and overall-modified Au-TDNN degradation by DNase I. (C, D) Fluorescence time graph depicting terminal-modified Au-TDNN and overall-modified Au-TDNN degradation by Exo III.

    Journal: Theranostics

    Article Title: High-Discrimination Factor Nanosensor Based on Tetrahedral DNA Nanostructures and Gold Nanoparticles for Detection of MiRNA-21 in Live Cells

    doi: 10.7150/thno.23852

    Figure Lengend Snippet: Enzymatic resistance of phosphorothioate-modified Au-TDNNs. (A, B) Fluorescence time graph depicting terminal-modified Au-TDNN and overall-modified Au-TDNN degradation by DNase I. (C, D) Fluorescence time graph depicting terminal-modified Au-TDNN and overall-modified Au-TDNN degradation by Exo III.

    Article Snippet: For both human DNase I (Thermo Scientific) and exonuclease III (New England Biolabs) digestion, a common concentration of 3 U/mL was used to digest 2 nM Au-TDNNs samples in PBS.

    Techniques: Modification, Fluorescence

    Enzymatic resistance of phosphorothioate-modified Au-TDNNs. (A, B) Fluorescence time graph depicting terminal-modified Au-TDNN and overall-modified Au-TDNN degradation by DNase I. (C, D) Fluorescence time graph depicting terminal-modified Au-TDNN and overall-modified Au-TDNN degradation by Exo III.

    Journal: Theranostics

    Article Title: High-Discrimination Factor Nanosensor Based on Tetrahedral DNA Nanostructures and Gold Nanoparticles for Detection of MiRNA-21 in Live Cells

    doi: 10.7150/thno.23852

    Figure Lengend Snippet: Enzymatic resistance of phosphorothioate-modified Au-TDNNs. (A, B) Fluorescence time graph depicting terminal-modified Au-TDNN and overall-modified Au-TDNN degradation by DNase I. (C, D) Fluorescence time graph depicting terminal-modified Au-TDNN and overall-modified Au-TDNN degradation by Exo III.

    Article Snippet: For both human DNase I (Thermo Scientific) and exonuclease III (New England Biolabs) digestion, a common concentration of 3 U/mL was used to digest 2 nM Au-TDNNs samples in PBS.

    Techniques: Modification, Fluorescence