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Genecopoeia human cygb
Knocking down iNOS diminishes the cytoprotective effect of overexpressing <t>CYGB.</t> ( a ) Representative FACS analysis with annexin V/PI staining showing H 2 O 2 -induced apoptosis in hCPCs stably expressing scrambled or iNOS <t>shRNA</t> following with or without CYGB overexpression. ( b ) Quantitative data analysis for panel a. ( c ) Representative images and quantitative data of Western blot showing the expression of anti-apoptotic and anti-oxidant proteins after iNOS was knocked down. Full-length blots are presented in Supplemental Fig. S16 . ( d ) Descriptive diagram of proposed molecular mechanism for CYGB regulated hCPC survival. *Indicates p
Human Cygb, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Cytoglobin Promotes Cardiac Progenitor Cell Survival against Oxidative Stress via the Upregulation of the NFκB/iNOS Signal Pathway and Nitric Oxide Production"

Article Title: Cytoglobin Promotes Cardiac Progenitor Cell Survival against Oxidative Stress via the Upregulation of the NFκB/iNOS Signal Pathway and Nitric Oxide Production

Journal: Scientific Reports

doi: 10.1038/s41598-017-11342-6

Knocking down iNOS diminishes the cytoprotective effect of overexpressing CYGB. ( a ) Representative FACS analysis with annexin V/PI staining showing H 2 O 2 -induced apoptosis in hCPCs stably expressing scrambled or iNOS shRNA following with or without CYGB overexpression. ( b ) Quantitative data analysis for panel a. ( c ) Representative images and quantitative data of Western blot showing the expression of anti-apoptotic and anti-oxidant proteins after iNOS was knocked down. Full-length blots are presented in Supplemental Fig. S16 . ( d ) Descriptive diagram of proposed molecular mechanism for CYGB regulated hCPC survival. *Indicates p
Figure Legend Snippet: Knocking down iNOS diminishes the cytoprotective effect of overexpressing CYGB. ( a ) Representative FACS analysis with annexin V/PI staining showing H 2 O 2 -induced apoptosis in hCPCs stably expressing scrambled or iNOS shRNA following with or without CYGB overexpression. ( b ) Quantitative data analysis for panel a. ( c ) Representative images and quantitative data of Western blot showing the expression of anti-apoptotic and anti-oxidant proteins after iNOS was knocked down. Full-length blots are presented in Supplemental Fig. S16 . ( d ) Descriptive diagram of proposed molecular mechanism for CYGB regulated hCPC survival. *Indicates p

Techniques Used: FACS, Staining, Stable Transfection, Expressing, shRNA, Over Expression, Western Blot

Overexpression of CYGB displays the anti-oxidant effect. ( a ) Representative images for DHE staining and quantitative analysis for hCPCs infected with CYGB- or vector-lentivirus for 48 hours. ( b ) Representative merged histogram and quantitative analysis for ROS measurement for hCPCs overexpressing CYGB vs. vector control by the FACS analysis with the ROS Detection Assay Kit (Deep Red Fluorescence). ( c ) Representative merged histogram and quantitative analysis for ROS measurement for hCPCs expressing shRNA against CYGB vs. scramble shRNA. The open blue traces are unstained cells as negative controls in panel b and c. ( d ) Examination of anti-oxidant gene expression at the mRNA level after CYGB was overexpressed. ( e ) Representative images and quantitative data of Western blot showing anti-oxidant protein expression level after CYGB was overexpressed. Full-length blots are presented in Supplemental Fig. 11 . *Indicates p
Figure Legend Snippet: Overexpression of CYGB displays the anti-oxidant effect. ( a ) Representative images for DHE staining and quantitative analysis for hCPCs infected with CYGB- or vector-lentivirus for 48 hours. ( b ) Representative merged histogram and quantitative analysis for ROS measurement for hCPCs overexpressing CYGB vs. vector control by the FACS analysis with the ROS Detection Assay Kit (Deep Red Fluorescence). ( c ) Representative merged histogram and quantitative analysis for ROS measurement for hCPCs expressing shRNA against CYGB vs. scramble shRNA. The open blue traces are unstained cells as negative controls in panel b and c. ( d ) Examination of anti-oxidant gene expression at the mRNA level after CYGB was overexpressed. ( e ) Representative images and quantitative data of Western blot showing anti-oxidant protein expression level after CYGB was overexpressed. Full-length blots are presented in Supplemental Fig. 11 . *Indicates p

Techniques Used: Over Expression, Staining, Infection, Plasmid Preparation, FACS, Detection Assay, Fluorescence, Expressing, shRNA, Western Blot

Disruption of NFκB expression abolished the cytoprotective effect of overexpressing CYGB. ( a ) Representative FACS analysis with annexin V/PI staining showing H 2 O 2 -induced apoptosis in hCPCs stably expressing scrambled or NFκB-p65 shRNA following with or without CYGB overexpression. ( b ) Quantitative data analysis for panel a. ( c ) Representative images and quantitative data of Western blot showing NFκB-related protein expression levels after NFκB was knocked-down. Full-length blots are presented in Supplemental Fig. S13 . ( d ) Representative images and quantitative data of Western blot showing anti-apoptotic and anti-oxidant protein expression levels after NFκB was knocked down. Full-length blots are presented in Supplemental Fig. S14 . *Indicates p
Figure Legend Snippet: Disruption of NFκB expression abolished the cytoprotective effect of overexpressing CYGB. ( a ) Representative FACS analysis with annexin V/PI staining showing H 2 O 2 -induced apoptosis in hCPCs stably expressing scrambled or NFκB-p65 shRNA following with or without CYGB overexpression. ( b ) Quantitative data analysis for panel a. ( c ) Representative images and quantitative data of Western blot showing NFκB-related protein expression levels after NFκB was knocked-down. Full-length blots are presented in Supplemental Fig. S13 . ( d ) Representative images and quantitative data of Western blot showing anti-apoptotic and anti-oxidant protein expression levels after NFκB was knocked down. Full-length blots are presented in Supplemental Fig. S14 . *Indicates p

Techniques Used: Expressing, FACS, Staining, Stable Transfection, shRNA, Over Expression, Western Blot

The expression of CYGB is associated with the cell survival ability against oxidative stress in hCPCs. ( a ) Representative images and quantitative data of Western blot showed that the expression of CYGB is observed in both hCPCs and human right atrial appendage (hRAA). However, the expression of myoglobin is not detectable in hCPCs compared to its abundant expression in hRAA. The total protein loading is 5 µg for hRAA and 20 µg for hCPCs. Full-length blots are presented in Supplemental Fig. S1 . ( b ) Western blot confirmed that CYGB was overexpressed in hCPCs. Full-length blots are presented in Supplemental Fig. S1 . ( c ) CPCs were infected with lentivirus expressing CYGB or vector for 24 h, challenged with 2 mM H 2 O 2 for 3 h, and then evaluated by LDH assay. ( d ) Western blot confirmed that CYGB was knocked-down in hCPCs with shRNAs for clone #1 4. Full-length blots are presented in Supplemental Fig. S8 . ( e ) Human CPCs were infected with lentivirus expressing shRNA against CYGB, or scramble shRNA for 24 h, challenged with 2 mM H 2 O 2 for 3 h, then evaluated by LDH assay. Data presented in this figure were mean values on a ratio of H 2 O 2 -induced LDH release to total LDH in cells with standard error (means ± SEM). *Indicates p
Figure Legend Snippet: The expression of CYGB is associated with the cell survival ability against oxidative stress in hCPCs. ( a ) Representative images and quantitative data of Western blot showed that the expression of CYGB is observed in both hCPCs and human right atrial appendage (hRAA). However, the expression of myoglobin is not detectable in hCPCs compared to its abundant expression in hRAA. The total protein loading is 5 µg for hRAA and 20 µg for hCPCs. Full-length blots are presented in Supplemental Fig. S1 . ( b ) Western blot confirmed that CYGB was overexpressed in hCPCs. Full-length blots are presented in Supplemental Fig. S1 . ( c ) CPCs were infected with lentivirus expressing CYGB or vector for 24 h, challenged with 2 mM H 2 O 2 for 3 h, and then evaluated by LDH assay. ( d ) Western blot confirmed that CYGB was knocked-down in hCPCs with shRNAs for clone #1 4. Full-length blots are presented in Supplemental Fig. S8 . ( e ) Human CPCs were infected with lentivirus expressing shRNA against CYGB, or scramble shRNA for 24 h, challenged with 2 mM H 2 O 2 for 3 h, then evaluated by LDH assay. Data presented in this figure were mean values on a ratio of H 2 O 2 -induced LDH release to total LDH in cells with standard error (means ± SEM). *Indicates p

Techniques Used: Expressing, Western Blot, Infection, Plasmid Preparation, Lactate Dehydrogenase Assay, shRNA

Related Articles

Infection:

Article Title: Cytoglobin Promotes Cardiac Progenitor Cell Survival against Oxidative Stress via the Upregulation of the NFκB/iNOS Signal Pathway and Nitric Oxide Production
Article Snippet: .. Lentiviral infection of hCPCs ORF expression clone for CYGB (purified plasmid), empty control vector for pReceiver-Lv202, shRNA scrambled control clone for psi-LVRH1GP, and shRNA clone set against human CYGB were purchased from GeneCopoeia. .. Lentiviruses were packaged by Lenti-Pac FIV expression packaging kit according to the manufacturer’s instructions (GeneCopoeia).

shRNA:

Article Title: Cytoglobin Promotes Cardiac Progenitor Cell Survival against Oxidative Stress via the Upregulation of the NFκB/iNOS Signal Pathway and Nitric Oxide Production
Article Snippet: .. Lentiviral infection of hCPCs ORF expression clone for CYGB (purified plasmid), empty control vector for pReceiver-Lv202, shRNA scrambled control clone for psi-LVRH1GP, and shRNA clone set against human CYGB were purchased from GeneCopoeia. .. Lentiviruses were packaged by Lenti-Pac FIV expression packaging kit according to the manufacturer’s instructions (GeneCopoeia).

Expressing:

Article Title: Cytoglobin Promotes Cardiac Progenitor Cell Survival against Oxidative Stress via the Upregulation of the NFκB/iNOS Signal Pathway and Nitric Oxide Production
Article Snippet: .. Lentiviral infection of hCPCs ORF expression clone for CYGB (purified plasmid), empty control vector for pReceiver-Lv202, shRNA scrambled control clone for psi-LVRH1GP, and shRNA clone set against human CYGB were purchased from GeneCopoeia. .. Lentiviruses were packaged by Lenti-Pac FIV expression packaging kit according to the manufacturer’s instructions (GeneCopoeia).

Plasmid Preparation:

Article Title: Cytoglobin Promotes Cardiac Progenitor Cell Survival against Oxidative Stress via the Upregulation of the NFκB/iNOS Signal Pathway and Nitric Oxide Production
Article Snippet: .. Lentiviral infection of hCPCs ORF expression clone for CYGB (purified plasmid), empty control vector for pReceiver-Lv202, shRNA scrambled control clone for psi-LVRH1GP, and shRNA clone set against human CYGB were purchased from GeneCopoeia. .. Lentiviruses were packaged by Lenti-Pac FIV expression packaging kit according to the manufacturer’s instructions (GeneCopoeia).

Purification:

Article Title: Cytoglobin Promotes Cardiac Progenitor Cell Survival against Oxidative Stress via the Upregulation of the NFκB/iNOS Signal Pathway and Nitric Oxide Production
Article Snippet: .. Lentiviral infection of hCPCs ORF expression clone for CYGB (purified plasmid), empty control vector for pReceiver-Lv202, shRNA scrambled control clone for psi-LVRH1GP, and shRNA clone set against human CYGB were purchased from GeneCopoeia. .. Lentiviruses were packaged by Lenti-Pac FIV expression packaging kit according to the manufacturer’s instructions (GeneCopoeia).

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    Genecopoeia human cygb
    Knocking down iNOS diminishes the cytoprotective effect of overexpressing <t>CYGB.</t> ( a ) Representative FACS analysis with annexin V/PI staining showing H 2 O 2 -induced apoptosis in hCPCs stably expressing scrambled or iNOS <t>shRNA</t> following with or without CYGB overexpression. ( b ) Quantitative data analysis for panel a. ( c ) Representative images and quantitative data of Western blot showing the expression of anti-apoptotic and anti-oxidant proteins after iNOS was knocked down. Full-length blots are presented in Supplemental Fig. S16 . ( d ) Descriptive diagram of proposed molecular mechanism for CYGB regulated hCPC survival. *Indicates p
    Human Cygb, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human cygb/product/Genecopoeia
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human cygb - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

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    Knocking down iNOS diminishes the cytoprotective effect of overexpressing CYGB. ( a ) Representative FACS analysis with annexin V/PI staining showing H 2 O 2 -induced apoptosis in hCPCs stably expressing scrambled or iNOS shRNA following with or without CYGB overexpression. ( b ) Quantitative data analysis for panel a. ( c ) Representative images and quantitative data of Western blot showing the expression of anti-apoptotic and anti-oxidant proteins after iNOS was knocked down. Full-length blots are presented in Supplemental Fig. S16 . ( d ) Descriptive diagram of proposed molecular mechanism for CYGB regulated hCPC survival. *Indicates p

    Journal: Scientific Reports

    Article Title: Cytoglobin Promotes Cardiac Progenitor Cell Survival against Oxidative Stress via the Upregulation of the NFκB/iNOS Signal Pathway and Nitric Oxide Production

    doi: 10.1038/s41598-017-11342-6

    Figure Lengend Snippet: Knocking down iNOS diminishes the cytoprotective effect of overexpressing CYGB. ( a ) Representative FACS analysis with annexin V/PI staining showing H 2 O 2 -induced apoptosis in hCPCs stably expressing scrambled or iNOS shRNA following with or without CYGB overexpression. ( b ) Quantitative data analysis for panel a. ( c ) Representative images and quantitative data of Western blot showing the expression of anti-apoptotic and anti-oxidant proteins after iNOS was knocked down. Full-length blots are presented in Supplemental Fig. S16 . ( d ) Descriptive diagram of proposed molecular mechanism for CYGB regulated hCPC survival. *Indicates p

    Article Snippet: Lentiviral infection of hCPCs ORF expression clone for CYGB (purified plasmid), empty control vector for pReceiver-Lv202, shRNA scrambled control clone for psi-LVRH1GP, and shRNA clone set against human CYGB were purchased from GeneCopoeia.

    Techniques: FACS, Staining, Stable Transfection, Expressing, shRNA, Over Expression, Western Blot

    Overexpression of CYGB displays the anti-oxidant effect. ( a ) Representative images for DHE staining and quantitative analysis for hCPCs infected with CYGB- or vector-lentivirus for 48 hours. ( b ) Representative merged histogram and quantitative analysis for ROS measurement for hCPCs overexpressing CYGB vs. vector control by the FACS analysis with the ROS Detection Assay Kit (Deep Red Fluorescence). ( c ) Representative merged histogram and quantitative analysis for ROS measurement for hCPCs expressing shRNA against CYGB vs. scramble shRNA. The open blue traces are unstained cells as negative controls in panel b and c. ( d ) Examination of anti-oxidant gene expression at the mRNA level after CYGB was overexpressed. ( e ) Representative images and quantitative data of Western blot showing anti-oxidant protein expression level after CYGB was overexpressed. Full-length blots are presented in Supplemental Fig. 11 . *Indicates p

    Journal: Scientific Reports

    Article Title: Cytoglobin Promotes Cardiac Progenitor Cell Survival against Oxidative Stress via the Upregulation of the NFκB/iNOS Signal Pathway and Nitric Oxide Production

    doi: 10.1038/s41598-017-11342-6

    Figure Lengend Snippet: Overexpression of CYGB displays the anti-oxidant effect. ( a ) Representative images for DHE staining and quantitative analysis for hCPCs infected with CYGB- or vector-lentivirus for 48 hours. ( b ) Representative merged histogram and quantitative analysis for ROS measurement for hCPCs overexpressing CYGB vs. vector control by the FACS analysis with the ROS Detection Assay Kit (Deep Red Fluorescence). ( c ) Representative merged histogram and quantitative analysis for ROS measurement for hCPCs expressing shRNA against CYGB vs. scramble shRNA. The open blue traces are unstained cells as negative controls in panel b and c. ( d ) Examination of anti-oxidant gene expression at the mRNA level after CYGB was overexpressed. ( e ) Representative images and quantitative data of Western blot showing anti-oxidant protein expression level after CYGB was overexpressed. Full-length blots are presented in Supplemental Fig. 11 . *Indicates p

    Article Snippet: Lentiviral infection of hCPCs ORF expression clone for CYGB (purified plasmid), empty control vector for pReceiver-Lv202, shRNA scrambled control clone for psi-LVRH1GP, and shRNA clone set against human CYGB were purchased from GeneCopoeia.

    Techniques: Over Expression, Staining, Infection, Plasmid Preparation, FACS, Detection Assay, Fluorescence, Expressing, shRNA, Western Blot

    Disruption of NFκB expression abolished the cytoprotective effect of overexpressing CYGB. ( a ) Representative FACS analysis with annexin V/PI staining showing H 2 O 2 -induced apoptosis in hCPCs stably expressing scrambled or NFκB-p65 shRNA following with or without CYGB overexpression. ( b ) Quantitative data analysis for panel a. ( c ) Representative images and quantitative data of Western blot showing NFκB-related protein expression levels after NFκB was knocked-down. Full-length blots are presented in Supplemental Fig. S13 . ( d ) Representative images and quantitative data of Western blot showing anti-apoptotic and anti-oxidant protein expression levels after NFκB was knocked down. Full-length blots are presented in Supplemental Fig. S14 . *Indicates p

    Journal: Scientific Reports

    Article Title: Cytoglobin Promotes Cardiac Progenitor Cell Survival against Oxidative Stress via the Upregulation of the NFκB/iNOS Signal Pathway and Nitric Oxide Production

    doi: 10.1038/s41598-017-11342-6

    Figure Lengend Snippet: Disruption of NFκB expression abolished the cytoprotective effect of overexpressing CYGB. ( a ) Representative FACS analysis with annexin V/PI staining showing H 2 O 2 -induced apoptosis in hCPCs stably expressing scrambled or NFκB-p65 shRNA following with or without CYGB overexpression. ( b ) Quantitative data analysis for panel a. ( c ) Representative images and quantitative data of Western blot showing NFκB-related protein expression levels after NFκB was knocked-down. Full-length blots are presented in Supplemental Fig. S13 . ( d ) Representative images and quantitative data of Western blot showing anti-apoptotic and anti-oxidant protein expression levels after NFκB was knocked down. Full-length blots are presented in Supplemental Fig. S14 . *Indicates p

    Article Snippet: Lentiviral infection of hCPCs ORF expression clone for CYGB (purified plasmid), empty control vector for pReceiver-Lv202, shRNA scrambled control clone for psi-LVRH1GP, and shRNA clone set against human CYGB were purchased from GeneCopoeia.

    Techniques: Expressing, FACS, Staining, Stable Transfection, shRNA, Over Expression, Western Blot

    The expression of CYGB is associated with the cell survival ability against oxidative stress in hCPCs. ( a ) Representative images and quantitative data of Western blot showed that the expression of CYGB is observed in both hCPCs and human right atrial appendage (hRAA). However, the expression of myoglobin is not detectable in hCPCs compared to its abundant expression in hRAA. The total protein loading is 5 µg for hRAA and 20 µg for hCPCs. Full-length blots are presented in Supplemental Fig. S1 . ( b ) Western blot confirmed that CYGB was overexpressed in hCPCs. Full-length blots are presented in Supplemental Fig. S1 . ( c ) CPCs were infected with lentivirus expressing CYGB or vector for 24 h, challenged with 2 mM H 2 O 2 for 3 h, and then evaluated by LDH assay. ( d ) Western blot confirmed that CYGB was knocked-down in hCPCs with shRNAs for clone #1 4. Full-length blots are presented in Supplemental Fig. S8 . ( e ) Human CPCs were infected with lentivirus expressing shRNA against CYGB, or scramble shRNA for 24 h, challenged with 2 mM H 2 O 2 for 3 h, then evaluated by LDH assay. Data presented in this figure were mean values on a ratio of H 2 O 2 -induced LDH release to total LDH in cells with standard error (means ± SEM). *Indicates p

    Journal: Scientific Reports

    Article Title: Cytoglobin Promotes Cardiac Progenitor Cell Survival against Oxidative Stress via the Upregulation of the NFκB/iNOS Signal Pathway and Nitric Oxide Production

    doi: 10.1038/s41598-017-11342-6

    Figure Lengend Snippet: The expression of CYGB is associated with the cell survival ability against oxidative stress in hCPCs. ( a ) Representative images and quantitative data of Western blot showed that the expression of CYGB is observed in both hCPCs and human right atrial appendage (hRAA). However, the expression of myoglobin is not detectable in hCPCs compared to its abundant expression in hRAA. The total protein loading is 5 µg for hRAA and 20 µg for hCPCs. Full-length blots are presented in Supplemental Fig. S1 . ( b ) Western blot confirmed that CYGB was overexpressed in hCPCs. Full-length blots are presented in Supplemental Fig. S1 . ( c ) CPCs were infected with lentivirus expressing CYGB or vector for 24 h, challenged with 2 mM H 2 O 2 for 3 h, and then evaluated by LDH assay. ( d ) Western blot confirmed that CYGB was knocked-down in hCPCs with shRNAs for clone #1 4. Full-length blots are presented in Supplemental Fig. S8 . ( e ) Human CPCs were infected with lentivirus expressing shRNA against CYGB, or scramble shRNA for 24 h, challenged with 2 mM H 2 O 2 for 3 h, then evaluated by LDH assay. Data presented in this figure were mean values on a ratio of H 2 O 2 -induced LDH release to total LDH in cells with standard error (means ± SEM). *Indicates p

    Article Snippet: Lentiviral infection of hCPCs ORF expression clone for CYGB (purified plasmid), empty control vector for pReceiver-Lv202, shRNA scrambled control clone for psi-LVRH1GP, and shRNA clone set against human CYGB were purchased from GeneCopoeia.

    Techniques: Expressing, Western Blot, Infection, Plasmid Preparation, Lactate Dehydrogenase Assay, shRNA