Journal: eLife

Article Title: CRMP4-mediated fornix development involves Semaphorin-3E signaling pathway

doi: 10.7554/eLife.70361

Figure Lengend Snippet: ( A ) Coronal sections of embryonic day 17.5 (E17.5) wild-type (WT) (columns a , c, d ) and CRMP4-KO (columns b , e ) brain were immunolabeled with anti-CRMP4 and anti-Nrp1 antibodies. White squares (columns a and b) indicate the positions of fields of view shown in columns c–e. Abbreviations: ac: anterior commissure, fi: fimbria; pf: post-commissural fornix. Scale bars, 100 µm. ( B ) Cultured subicular neurons at 2 days in vitro (DIV) were immunolabeled with anti-CRMP4 and anti-Nrp1 antibodies. The conventional confocal plane shows the entire neuron. Images from the confocal Airyscan detail the growth cone on two serial acquisition planes. Scale bars, 10 µm.

Article Snippet: The plasmid coding for human CRMP4-tGFP (aa 1–570, in pCMV6-AC-tGFP) was from Origene (Rockville, MD) (RG230865, # NM_001197294, NP_001184223).

Techniques: Immunolabeling, Cell Culture, In Vitro

Journal: eLife

Article Title: CRMP4-mediated fornix development involves Semaphorin-3E signaling pathway

doi: 10.7554/eLife.70361

Figure Lengend Snippet: ( A ) Schematic representation of the fornix tract (red region) on a sagittal diagram. Grey lines indicate the Bregma positions, in mm, of the coronal planes presented in B, C, and D. ( B–D ) Coronal brain sections from adult wild-type (WT) and CRMP4-knockout (KO) brains were stained with gold chloride. Diagrams of adult brain coronal slices illustrating the anatomical levels selected to measure surfaces covered by the fornix (pf) and mammillo-thalamic tract (mt). Square in the diagrams indicate the regions shown on the coronal sections stained with gold chloride. Histograms show quantifications of fiber areas and fasciculation index, mean ± s.e.m. WT = 5, KO = 4. Mann-Whitney test, *p < 0.05. Scale bar, 250 µm. Abbreviations: HF: hippocampal formation; MB: mammillary body; AH: anterior hypothalamus; SPT: septum; pre-f: pre-commissural fornix; pf: post-commissural fornix; mt: mammillo-thalamic tract; mcht, medial cortico-hypothalamic tract; ac: anterior commissure; S: subiculum. Figure 5—source data 1. Raw data of panels A-D.

Article Snippet: The plasmid coding for human CRMP4-tGFP (aa 1–570, in pCMV6-AC-tGFP) was from Origene (Rockville, MD) (RG230865, # NM_001197294, NP_001184223).

Techniques: Knock-Out, Staining, MANN-WHITNEY

Journal: eLife

Article Title: CRMP4-mediated fornix development involves Semaphorin-3E signaling pathway

doi: 10.7554/eLife.70361

Figure Lengend Snippet: Coronal sections of the subiculum from adult WT and CRMP4-knockout (KO) animals expressing the reporter eYFP in the subiculum. The dorsal subiculum shown in the diagram of Bregma –2.80 mm section ( A ) was delimited on the NeuN immunolabeling slice (B, dashed region of interest [ROI]). The density of eYFP-stained subicular neurons in WT and CRMP4-KO dorsal subicula was quantified, and the results are shown in ( C ). Difference between genotypes was not significant (n = 4 animals for each genotype).

Article Snippet: The plasmid coding for human CRMP4-tGFP (aa 1–570, in pCMV6-AC-tGFP) was from Origene (Rockville, MD) (RG230865, # NM_001197294, NP_001184223).

Techniques: Knock-Out, Expressing, Immunolabeling, Staining

Journal: eLife

Article Title: CRMP4-mediated fornix development involves Semaphorin-3E signaling pathway

doi: 10.7554/eLife.70361

Figure Lengend Snippet: ( A ) Adult half-brains from wild-type (WT)- and CRMP4-KO-Thy1-eYFP-H mice were cleared by a hybrid uDISCO method (see Material and methods section). Representative broad panoramic views of z projections of WT and CRMP4-KO are shown. White squares indicate regions shown in B. Scale bar, 1 mm. Voxel size, x = 2.07, y = 2.07, z = 5.77 µm. ( B ) Higher magnifications of z projections show post-commissural fornix fibers (pf) entering mammillary bodies (MB) and mcht fibers emerging from the fornix. Arrows and arrowheads indicate the mcht fibers emerging from the anterior and posterior sides, respectively, of the anterior commissure (ac). Scale bar, 200 µm. Abbreviations: HF: hippocampal formation, MB: mammillary body; S: subiculum; SPT: septum; ac: anterior commissure; mcht: medial cortico-hypothalamic tract; pf: post-commissural fornix; and pre-f: pre-commissural fornix.

Article Snippet: The plasmid coding for human CRMP4-tGFP (aa 1–570, in pCMV6-AC-tGFP) was from Origene (Rockville, MD) (RG230865, # NM_001197294, NP_001184223).

Techniques: