human cord blood mononuclear cells mncs  (Millipore)

 
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    Millipore human cord blood mononuclear cells mncs
    Human Cord Blood Mononuclear Cells Mncs, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human cord blood mononuclear cells mncs/product/Millipore
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human cord blood mononuclear cells mncs - by Bioz Stars, 2021-03
    95/100 stars

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    In Vitro:

    Article Title: Dermotropic Leishmania donovani in Sri Lanka: visceralizing potential in clinical and preclinical studies
    Article Snippet: IFN-γ and IL-10 cytokine concentrations in culture supernatants were determined by sandwich ELISA, according to the manufacturer's instructions (R & D SYSTEMS, Minneapolis, USA). .. In vitro infection of human macrophages with L. donovani promastigotes Human mononuclear cells were isolated from peripheral blood of normal healthy donors by density gradient sedimentation in Ficoll-Hypaque (Sigma Chemical Co. St. Louis, MO). ..

    Article Title: Synthesis and structure-activity relationships of novel 5-(hydroxamic acid)methyl oxazolidinone derivatives as 5-lipoxygenase inhibitors
    Article Snippet: .. In vitro assay for the inhibition of 5-LO product LTC4 from isolated human monocytes Mononuclear cells were first isolated from heparinised blood using the Ficoll-Hypaque gradient centrifugation method. .. The purity of the adherent monocytes (CD14+) was routinely confirmed by flow cytometry to be > 95% and viability (by trypan blue exclusion method) was routinely > 97%.

    Infection:

    Article Title: Dermotropic Leishmania donovani in Sri Lanka: visceralizing potential in clinical and preclinical studies
    Article Snippet: IFN-γ and IL-10 cytokine concentrations in culture supernatants were determined by sandwich ELISA, according to the manufacturer's instructions (R & D SYSTEMS, Minneapolis, USA). .. In vitro infection of human macrophages with L. donovani promastigotes Human mononuclear cells were isolated from peripheral blood of normal healthy donors by density gradient sedimentation in Ficoll-Hypaque (Sigma Chemical Co. St. Louis, MO). ..

    Isolation:

    Article Title: Dermotropic Leishmania donovani in Sri Lanka: visceralizing potential in clinical and preclinical studies
    Article Snippet: IFN-γ and IL-10 cytokine concentrations in culture supernatants were determined by sandwich ELISA, according to the manufacturer's instructions (R & D SYSTEMS, Minneapolis, USA). .. In vitro infection of human macrophages with L. donovani promastigotes Human mononuclear cells were isolated from peripheral blood of normal healthy donors by density gradient sedimentation in Ficoll-Hypaque (Sigma Chemical Co. St. Louis, MO). ..

    Article Title: Protective and Detrimental Roles for Regulatory T Cells in a Viral Model for Multiple Sclerosis
    Article Snippet: Absorbance was read at 492 nm on a Multiskan MCC/340 (Thermo Scientific, Rochester, NY). .. Spleens were removed from TMEV-infected mice during the acute or chronic stage of disease, mononuclear cells (MNCs) were isolated using Histopaque® 1083 (Sigma-Aldrich) ( ). .. A volume of 100 μl of 2 × 105 MNCs in RPMI 1640 supplemented with 1% glutamine, 1% antibiotics, 50 μM 2-mercaptoethanol, and 10% FBS was added to each well of 96-well plates.

    Article Title: Synthesis and structure-activity relationships of novel 5-(hydroxamic acid)methyl oxazolidinone derivatives as 5-lipoxygenase inhibitors
    Article Snippet: .. In vitro assay for the inhibition of 5-LO product LTC4 from isolated human monocytes Mononuclear cells were first isolated from heparinised blood using the Ficoll-Hypaque gradient centrifugation method. .. The purity of the adherent monocytes (CD14+) was routinely confirmed by flow cytometry to be > 95% and viability (by trypan blue exclusion method) was routinely > 97%.

    Article Title: Optimization of culture conditions for rapid clinical-scale expansion of human umbilical cord blood-derived mesenchymal stem cells
    Article Snippet: Isolation of hUCB-MSCs and culture conditions hUCB-MSCs were collected from the umbilical cord vein of a newborn baby, with the consent of the mother. .. To isolate and expand MSCs from cord blood, mononuclear cells (MNCs) were isolated using a Ficoll–Hypaque solution (d = 1.077 g/cm3 ; Sigma). ..

    Article Title: Enhancement of peptide vaccine immunogenicity by increasing lymphatic drainage and boosting serum stability
    Article Snippet: The human HLA-B57-restricted KF11-specific CD8+ T-cell clone (recognizing KAFSPEVIPMF ( )) was maintained in the presence of IL2 (50 U/mL, R & D Systems), using CD3-specific monoclonal antibody 12F6 (0.1 µg/mL, Enzo Life Sciences), and irradiated allogeneic feeder PBMCs. .. Human peripheral blood mononuclear cells (PBMCs) were freshly isolated from HLA-B57+ blood donors with Ficoll-Hypaque (Sigma-Aldrich). .. PBMCs were isolated from buffy coats collected from HLA-typed blood donors after written informed consent and approval by the Partners Human Research Committee (protocol #2005P001218).

    Article Title: MicroRNA expression profiling of Leishmania donovani-infected host cells uncovers the regulatory role of MIR30A-3p in host autophagy
    Article Snippet: Cells were treated with 50 ng/ml phorbol 12-myristate 13-acetate (Sigma-Aldrich, P1585) for 48 h to induce differentiation into macrophage-like cells before infection. .. Primary human peripheral blood mononuclear cells were isolated using a Ficoll Paque density gradient (Sigma-Aldrich, GE-17-5442-02) on whole blood collected from healthy donors. .. Human monocyte-derived macrophages (HsMDMs) were isolated as described previously.

    Sedimentation:

    Article Title: Dermotropic Leishmania donovani in Sri Lanka: visceralizing potential in clinical and preclinical studies
    Article Snippet: IFN-γ and IL-10 cytokine concentrations in culture supernatants were determined by sandwich ELISA, according to the manufacturer's instructions (R & D SYSTEMS, Minneapolis, USA). .. In vitro infection of human macrophages with L. donovani promastigotes Human mononuclear cells were isolated from peripheral blood of normal healthy donors by density gradient sedimentation in Ficoll-Hypaque (Sigma Chemical Co. St. Louis, MO). ..

    Mouse Assay:

    Article Title: Protective and Detrimental Roles for Regulatory T Cells in a Viral Model for Multiple Sclerosis
    Article Snippet: Absorbance was read at 492 nm on a Multiskan MCC/340 (Thermo Scientific, Rochester, NY). .. Spleens were removed from TMEV-infected mice during the acute or chronic stage of disease, mononuclear cells (MNCs) were isolated using Histopaque® 1083 (Sigma-Aldrich) ( ). .. A volume of 100 μl of 2 × 105 MNCs in RPMI 1640 supplemented with 1% glutamine, 1% antibiotics, 50 μM 2-mercaptoethanol, and 10% FBS was added to each well of 96-well plates.

    Inhibition:

    Article Title: Synthesis and structure-activity relationships of novel 5-(hydroxamic acid)methyl oxazolidinone derivatives as 5-lipoxygenase inhibitors
    Article Snippet: .. In vitro assay for the inhibition of 5-LO product LTC4 from isolated human monocytes Mononuclear cells were first isolated from heparinised blood using the Ficoll-Hypaque gradient centrifugation method. .. The purity of the adherent monocytes (CD14+) was routinely confirmed by flow cytometry to be > 95% and viability (by trypan blue exclusion method) was routinely > 97%.

    Gradient Centrifugation:

    Article Title: Synthesis and structure-activity relationships of novel 5-(hydroxamic acid)methyl oxazolidinone derivatives as 5-lipoxygenase inhibitors
    Article Snippet: .. In vitro assay for the inhibition of 5-LO product LTC4 from isolated human monocytes Mononuclear cells were first isolated from heparinised blood using the Ficoll-Hypaque gradient centrifugation method. .. The purity of the adherent monocytes (CD14+) was routinely confirmed by flow cytometry to be > 95% and viability (by trypan blue exclusion method) was routinely > 97%.

    Binding Assay:

    Article Title: Pharmacology of AMG 181, a human anti-?4?7 antibody that specifically alters trafficking of gut-homing T cells
    Article Snippet: .. For primary human T cell binding assays, human peripheral blood mononuclear cells (PBMCs) were stained with serially diluted, biotinylated AMG 181 in the binding buffer (30 mM HEPES, 140 mM NaCl and 1% BSA) (Sigma-Aldrich, St. Louis, MO, USA). .. Binding of AMG 181 to human CD4+CD45RA-memory T cells was assessed by FACS analysis.

    Staining:

    Article Title: Pharmacology of AMG 181, a human anti-?4?7 antibody that specifically alters trafficking of gut-homing T cells
    Article Snippet: .. For primary human T cell binding assays, human peripheral blood mononuclear cells (PBMCs) were stained with serially diluted, biotinylated AMG 181 in the binding buffer (30 mM HEPES, 140 mM NaCl and 1% BSA) (Sigma-Aldrich, St. Louis, MO, USA). .. Binding of AMG 181 to human CD4+CD45RA-memory T cells was assessed by FACS analysis.

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    • mononuclear cells mncs  (Millipore)
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    Millipore mononuclear cells mncs
    Ca 2+ /hypoxia-treated <t>hUCB-MSCs</t> maintained their proliferation capacity in long-term culture. a , b Growth curves and population doubling (PD) time of hUCB-MSCs in individual treatments were analyzed for all passages during long-term culture. c , d After isolation of <t>MNCs</t> from UCB, the cells were maintained under the two culture conditions. Growth curves and PD time were analyzed for hUCB-MSCs treated with Ca 2+ /hypoxia through a series of passages. The initial cell concentration of hUCB-MSCs was 5 × 10 3 cells/cm 2 . Cell proliferation was measured by cell counting assays. Data represent the mean ± SD, n = 3; * P
    Mononuclear Cells Mncs, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mononuclear cells mncs/product/Millipore
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mononuclear cells mncs - by Bioz Stars, 2021-03
    95/100 stars
    		  Buy from Supplier
    mono nuclear cells mncs  (Millipore)
    99
    Millipore mono nuclear cells mncs
    Ca 2+ /hypoxia-treated <t>hUCB-MSCs</t> maintained their proliferation capacity in long-term culture. a , b Growth curves and population doubling (PD) time of hUCB-MSCs in individual treatments were analyzed for all passages during long-term culture. c , d After isolation of <t>MNCs</t> from UCB, the cells were maintained under the two culture conditions. Growth curves and PD time were analyzed for hUCB-MSCs treated with Ca 2+ /hypoxia through a series of passages. The initial cell concentration of hUCB-MSCs was 5 × 10 3 cells/cm 2 . Cell proliferation was measured by cell counting assays. Data represent the mean ± SD, n = 3; * P
    Mono Nuclear Cells Mncs, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mono nuclear cells mncs/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mono nuclear cells mncs - by Bioz Stars, 2021-03
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Ca 2+ /hypoxia-treated hUCB-MSCs maintained their proliferation capacity in long-term culture. a , b Growth curves and population doubling (PD) time of hUCB-MSCs in individual treatments were analyzed for all passages during long-term culture. c , d After isolation of MNCs from UCB, the cells were maintained under the two culture conditions. Growth curves and PD time were analyzed for hUCB-MSCs treated with Ca 2+ /hypoxia through a series of passages. The initial cell concentration of hUCB-MSCs was 5 × 10 3 cells/cm 2 . Cell proliferation was measured by cell counting assays. Data represent the mean ± SD, n = 3; * P

    Journal: Clinical and Translational Medicine

    Article Title: Optimization of culture conditions for rapid clinical-scale expansion of human umbilical cord blood-derived mesenchymal stem cells

    doi: 10.1186/s40169-017-0168-z

    Figure Lengend Snippet: Ca 2+ /hypoxia-treated hUCB-MSCs maintained their proliferation capacity in long-term culture. a , b Growth curves and population doubling (PD) time of hUCB-MSCs in individual treatments were analyzed for all passages during long-term culture. c , d After isolation of MNCs from UCB, the cells were maintained under the two culture conditions. Growth curves and PD time were analyzed for hUCB-MSCs treated with Ca 2+ /hypoxia through a series of passages. The initial cell concentration of hUCB-MSCs was 5 × 10 3 cells/cm 2 . Cell proliferation was measured by cell counting assays. Data represent the mean ± SD, n = 3; * P

    Article Snippet: To isolate and expand MSCs from cord blood, mononuclear cells (MNCs) were isolated using a Ficoll–Hypaque solution (d = 1.077 g/cm3 ; Sigma).

    Techniques: Isolation, Concentration Assay, Cell Counting