human colorectal  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH human colorectal
    The effect of lichen-derived compounds on Nrf2 activation in DLD-1 and <t>HCT116</t> cells. ( A ) The level of Nrf2 protein in the cytosolic fraction. ( B ) The level of Nrf2 protein in the nuclear fraction. ( C ) The level of p-Nrf2 protein in the nuclear fraction. Representative Western immunoblots are presented under the graphs ( A – C ). Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin or lamin. ( D ) The level of Nrf2 binding to DNA. Results are presented as the means ± SEM from three separate experiments percentage of control). Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).
    Human Colorectal, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human colorectal - by Bioz Stars, 2024-05
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    1) Product Images from "Lichen-Derived Depsides and Depsidones Modulate the Nrf2, NF-κB and STAT3 Signaling Pathways in Colorectal Cancer Cells"

    Article Title: Lichen-Derived Depsides and Depsidones Modulate the Nrf2, NF-κB and STAT3 Signaling Pathways in Colorectal Cancer Cells

    Journal: Molecules

    doi: 10.3390/molecules26164787

    The effect of lichen-derived compounds on Nrf2 activation in DLD-1 and HCT116 cells. ( A ) The level of Nrf2 protein in the cytosolic fraction. ( B ) The level of Nrf2 protein in the nuclear fraction. ( C ) The level of p-Nrf2 protein in the nuclear fraction. Representative Western immunoblots are presented under the graphs ( A – C ). Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin or lamin. ( D ) The level of Nrf2 binding to DNA. Results are presented as the means ± SEM from three separate experiments percentage of control). Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).
    Figure Legend Snippet: The effect of lichen-derived compounds on Nrf2 activation in DLD-1 and HCT116 cells. ( A ) The level of Nrf2 protein in the cytosolic fraction. ( B ) The level of Nrf2 protein in the nuclear fraction. ( C ) The level of p-Nrf2 protein in the nuclear fraction. Representative Western immunoblots are presented under the graphs ( A – C ). Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin or lamin. ( D ) The level of Nrf2 binding to DNA. Results are presented as the means ± SEM from three separate experiments percentage of control). Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Techniques Used: Derivative Assay, Activation Assay, Western Blot, Binding Assay

    The effect of lichen-derived compounds on the expression of Nrf2 in DLD-1 and HCT116 cells. The values (fold of control) are presented as the means ± SEM from three separate experiments. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).
    Figure Legend Snippet: The effect of lichen-derived compounds on the expression of Nrf2 in DLD-1 and HCT116 cells. The values (fold of control) are presented as the means ± SEM from three separate experiments. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Techniques Used: Derivative Assay, Expressing

    The effect of lichen-derived compounds on the expression of selected Nrf2 target genes: SOD and GSTP in DLD-1 and HCT116 cells. ( A ) Levels of SOD and GSTP transcripts. The values (fold of control) are presented as the means ± SEM from three separate experiments. ( B ) Level of SOD and GSTP proteins. Representative Western immunoblots are presented under the graphs. Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).
    Figure Legend Snippet: The effect of lichen-derived compounds on the expression of selected Nrf2 target genes: SOD and GSTP in DLD-1 and HCT116 cells. ( A ) Levels of SOD and GSTP transcripts. The values (fold of control) are presented as the means ± SEM from three separate experiments. ( B ) Level of SOD and GSTP proteins. Representative Western immunoblots are presented under the graphs. Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Techniques Used: Derivative Assay, Expressing, Western Blot

    The effect of lichen-derived compounds on the expression of selected Nrf2 target genes: CAT and GPx in DLD-1 and HCT116 cells. ( A ) Levels of CAT and GPx transcripts. The values (fold of control) are presented as the means ± SEM from three separate experiments. ( B ) Levels of CAT and GPx proteins. Representative Western immunoblots are presented under the graphs. Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).
    Figure Legend Snippet: The effect of lichen-derived compounds on the expression of selected Nrf2 target genes: CAT and GPx in DLD-1 and HCT116 cells. ( A ) Levels of CAT and GPx transcripts. The values (fold of control) are presented as the means ± SEM from three separate experiments. ( B ) Levels of CAT and GPx proteins. Representative Western immunoblots are presented under the graphs. Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Techniques Used: Derivative Assay, Expressing, Western Blot

    The effect of lichen-derived compounds on NF-κB activation in DLD-1 and HCT116 cells. ( A ) The levels of NF-κB p50 and p65 proteins in the cytosolic fraction. ( B ) The levels of NF-κB p50 and p65 proteins in the nuclear fraction. Representative Western immunoblots are presented under the graphs ( A , B ). Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin or lamin. ( C ) The levels of NF-κB p50 and p65 binding to DNA. Results are presented as the means ± SEM from three separate experiments percentage of control). Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).
    Figure Legend Snippet: The effect of lichen-derived compounds on NF-κB activation in DLD-1 and HCT116 cells. ( A ) The levels of NF-κB p50 and p65 proteins in the cytosolic fraction. ( B ) The levels of NF-κB p50 and p65 proteins in the nuclear fraction. Representative Western immunoblots are presented under the graphs ( A , B ). Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin or lamin. ( C ) The levels of NF-κB p50 and p65 binding to DNA. Results are presented as the means ± SEM from three separate experiments percentage of control). Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Techniques Used: Derivative Assay, Activation Assay, Western Blot, Binding Assay

    The effect of lichen-derived compounds on the expression of NF-κB p50 and p65 in DLD-1 and HCT116 cells. The values (fold of control) are presented as the means ± SEM from three separate experiments. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).
    Figure Legend Snippet: The effect of lichen-derived compounds on the expression of NF-κB p50 and p65 in DLD-1 and HCT116 cells. The values (fold of control) are presented as the means ± SEM from three separate experiments. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Techniques Used: Derivative Assay, Expressing

    The effect of lichen-derived compounds on the expression of selected NF-κB target genes: COX-2 and iNOS in DLD-1 and HCT116 cells. ( A ) Levels of COX-2 and iNOS transcripts. The values (fold of control) are presented as the means ± SEM from three separate experiments. ( B ) Levels of COX-2 and iNOS proteins. Representative Western immunoblots are presented under the graphs. Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).
    Figure Legend Snippet: The effect of lichen-derived compounds on the expression of selected NF-κB target genes: COX-2 and iNOS in DLD-1 and HCT116 cells. ( A ) Levels of COX-2 and iNOS transcripts. The values (fold of control) are presented as the means ± SEM from three separate experiments. ( B ) Levels of COX-2 and iNOS proteins. Representative Western immunoblots are presented under the graphs. Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Techniques Used: Derivative Assay, Expressing, Western Blot

    The effect of lichen-derived compounds on STAT3 activation in DLD-1 and HCT116 cells. ( A ) The level of STAT3 protein in the cytosolic fraction. ( B ) The level of STAT3 protein in the nuclear fraction. Representative Western immunoblots are presented under the graphs ( A , B ). Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin or lamin. ( C ) The level of STAT3 binding to DNA. Results are presented as the means ± SEM from three separate experiments percentage of control). Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).
    Figure Legend Snippet: The effect of lichen-derived compounds on STAT3 activation in DLD-1 and HCT116 cells. ( A ) The level of STAT3 protein in the cytosolic fraction. ( B ) The level of STAT3 protein in the nuclear fraction. Representative Western immunoblots are presented under the graphs ( A , B ). Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin or lamin. ( C ) The level of STAT3 binding to DNA. Results are presented as the means ± SEM from three separate experiments percentage of control). Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Techniques Used: Derivative Assay, Activation Assay, Western Blot, Binding Assay

    The effect of lichen-derived compounds on the level of p-STAT3 in DLD-1 and HCT116 cells. ( A ) The level of p-STAT3 protein in the nuclear fraction. Representative Western immunoblots are presented under the graphs. Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of lamin. ( B ) The ratio of nuclear p-STAT3 and nuclear STAT3 compared with the control group. DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).
    Figure Legend Snippet: The effect of lichen-derived compounds on the level of p-STAT3 in DLD-1 and HCT116 cells. ( A ) The level of p-STAT3 protein in the nuclear fraction. Representative Western immunoblots are presented under the graphs. Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of lamin. ( B ) The ratio of nuclear p-STAT3 and nuclear STAT3 compared with the control group. DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Techniques Used: Derivative Assay, Western Blot

    The effect of lichen-derived compounds on the expression of STAT3 in DLD-1 and HCT116 cells. The values (fold of control) are presented as the means ± SEM from three separate experiments. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).
    Figure Legend Snippet: The effect of lichen-derived compounds on the expression of STAT3 in DLD-1 and HCT116 cells. The values (fold of control) are presented as the means ± SEM from three separate experiments. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Techniques Used: Derivative Assay, Expressing

    The effect of lichen-derived compounds on the expression of selected STAT3 target gene: Bcl-xl in DLD-1 and HCT116 cells. ( A ) Level of the Bcl-xl transcript. The values (fold of control) are presented as the means ± SEM from three separate experiments. ( B ) Level of the Bcl-xl protein. Representative Western immunoblots are presented under the graphs. Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).
    Figure Legend Snippet: The effect of lichen-derived compounds on the expression of selected STAT3 target gene: Bcl-xl in DLD-1 and HCT116 cells. ( A ) Level of the Bcl-xl transcript. The values (fold of control) are presented as the means ± SEM from three separate experiments. ( B ) Level of the Bcl-xl protein. Representative Western immunoblots are presented under the graphs. Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Techniques Used: Derivative Assay, Expressing, Western Blot

    human epithelial colorectal adenocarcinoma cells  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH human epithelial colorectal adenocarcinoma cells
    Human Epithelial Colorectal Adenocarcinoma Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human epithelial colorectal adenocarcinoma cells  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH human epithelial colorectal adenocarcinoma cells
    Human Epithelial Colorectal Adenocarcinoma Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human epithelial colorectal adenocarcinoma cells/product/CLS Cell Lines Service GmbH
    Average 86 stars, based on 1 article reviews
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    human colorectal cancer cell line ht  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH human colorectal cancer cell line ht
    (A) Measuring cell viability following short-term hyperthermia. In vitro exposure of colorectal cancer cells <t>(HT-29)</t> to short-term hyperthermia (10 sec). Exposure to temperature levels from 37˚C (control) in 5˚C step increments until 80˚C. An additional control with Oxa. At 37˚C was conducted to compare vitality levels with chemotherapy. (B) Measuring cytotoxicity following hyperthermia: In vitro exposure of colorectal cancer (HT-29) cells to short-term hyperthermia (10 sec). Exposure to temperature levels of 37˚C (Control) in 5˚C step increments until 80˚C. An additional control with Oxa. At 37˚C was conducted to help compare vitality levels with chemotherapy. # P>0.05, * P<0.05, ** P<0.01. Oxa., oxaliplatin; LDH, lactate dehydrogenase.
    Human Colorectal Cancer Cell Line Ht, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human colorectal cancer cell line ht/product/CLS Cell Lines Service GmbH
    Average 86 stars, based on 1 article reviews
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    1) Product Images from "Evaluating the concept of gas‑based intraperitoneal hyperthermia beyond 43˚C in the treatment of peritoneal metastasis: A pilot study"

    Article Title: Evaluating the concept of gas‑based intraperitoneal hyperthermia beyond 43˚C in the treatment of peritoneal metastasis: A pilot study

    Journal: Experimental and Therapeutic Medicine

    doi: 10.3892/etm.2022.11687

    (A) Measuring cell viability following short-term hyperthermia. In vitro exposure of colorectal cancer cells (HT-29) to short-term hyperthermia (10 sec). Exposure to temperature levels from 37˚C (control) in 5˚C step increments until 80˚C. An additional control with Oxa. At 37˚C was conducted to compare vitality levels with chemotherapy. (B) Measuring cytotoxicity following hyperthermia: In vitro exposure of colorectal cancer (HT-29) cells to short-term hyperthermia (10 sec). Exposure to temperature levels of 37˚C (Control) in 5˚C step increments until 80˚C. An additional control with Oxa. At 37˚C was conducted to help compare vitality levels with chemotherapy. # P>0.05, * P<0.05, ** P<0.01. Oxa., oxaliplatin; LDH, lactate dehydrogenase.
    Figure Legend Snippet: (A) Measuring cell viability following short-term hyperthermia. In vitro exposure of colorectal cancer cells (HT-29) to short-term hyperthermia (10 sec). Exposure to temperature levels from 37˚C (control) in 5˚C step increments until 80˚C. An additional control with Oxa. At 37˚C was conducted to compare vitality levels with chemotherapy. (B) Measuring cytotoxicity following hyperthermia: In vitro exposure of colorectal cancer (HT-29) cells to short-term hyperthermia (10 sec). Exposure to temperature levels of 37˚C (Control) in 5˚C step increments until 80˚C. An additional control with Oxa. At 37˚C was conducted to help compare vitality levels with chemotherapy. # P>0.05, * P<0.05, ** P<0.01. Oxa., oxaliplatin; LDH, lactate dehydrogenase.

    Techniques Used: In Vitro

    human colorectal carcinoma cells  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH human colorectal carcinoma cells
    <t>Colorectal</t> cancer <t>cells—HCT</t> 116 nuclei stained with Hoechst 33342 dye after a 24 h treatment with Mentha piperita L. essential oil—M_EO (10 and 150 μg/mL). Staurosporine (5 µM) was used as the positive control for apoptotic changes at nuclear level and Triton X-100 (0.5%) for necrosis. The yellow arrows indicate signs of apoptosis, and the red arrow shows signs of necrosis. The scale bar was 20 µm.
    Human Colorectal Carcinoma Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Chemical and Antimicrobial Characterization of Mentha piperita L. and Rosmarinus officinalis L. Essential Oils and In Vitro Potential Cytotoxic Effect in Human Colorectal Carcinoma Cells"

    Article Title: Chemical and Antimicrobial Characterization of Mentha piperita L. and Rosmarinus officinalis L. Essential Oils and In Vitro Potential Cytotoxic Effect in Human Colorectal Carcinoma Cells

    Journal: Molecules

    doi: 10.3390/molecules27186106

    Colorectal cancer cells—HCT 116 nuclei stained with Hoechst 33342 dye after a 24 h treatment with Mentha piperita L. essential oil—M_EO (10 and 150 μg/mL). Staurosporine (5 µM) was used as the positive control for apoptotic changes at nuclear level and Triton X-100 (0.5%) for necrosis. The yellow arrows indicate signs of apoptosis, and the red arrow shows signs of necrosis. The scale bar was 20 µm.
    Figure Legend Snippet: Colorectal cancer cells—HCT 116 nuclei stained with Hoechst 33342 dye after a 24 h treatment with Mentha piperita L. essential oil—M_EO (10 and 150 μg/mL). Staurosporine (5 µM) was used as the positive control for apoptotic changes at nuclear level and Triton X-100 (0.5%) for necrosis. The yellow arrows indicate signs of apoptosis, and the red arrow shows signs of necrosis. The scale bar was 20 µm.

    Techniques Used: Staining, Positive Control

    Colorectal cancer cells—HCT 116 nuclei stained with Hoechst 33342 dye after a 24 h treatment with Rosmarinus officinalis L. essential oil—R_EO (10 and 150 μg/mL). Staurosporine (5 µM) was used as the positive control for apoptotic changes at nuclear level and Triton X-100 (0.5%) for necrosis. The yellow arrows indicate signs of apoptosis, and the red arrow shows signs of necrosis. The scale bar was 20 µm.
    Figure Legend Snippet: Colorectal cancer cells—HCT 116 nuclei stained with Hoechst 33342 dye after a 24 h treatment with Rosmarinus officinalis L. essential oil—R_EO (10 and 150 μg/mL). Staurosporine (5 µM) was used as the positive control for apoptotic changes at nuclear level and Triton X-100 (0.5%) for necrosis. The yellow arrows indicate signs of apoptosis, and the red arrow shows signs of necrosis. The scale bar was 20 µm.

    Techniques Used: Staining, Positive Control

    human colorectal adenocarcinoma cell line hct15  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH human colorectal adenocarcinoma cell line hct15
    Human Colorectal Adenocarcinoma Cell Line Hct15, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human colorectal adenocarcinoma cell line hct15 - by Bioz Stars, 2024-05
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    human colorectal  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH human colorectal
    The effect of lichen-derived compounds on Nrf2 activation in DLD-1 and <t>HCT116</t> cells. ( A ) The level of Nrf2 protein in the cytosolic fraction. ( B ) The level of Nrf2 protein in the nuclear fraction. ( C ) The level of p-Nrf2 protein in the nuclear fraction. Representative Western immunoblots are presented under the graphs ( A – C ). Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin or lamin. ( D ) The level of Nrf2 binding to DNA. Results are presented as the means ± SEM from three separate experiments percentage of control). Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).
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    1) Product Images from "Lichen-Derived Depsides and Depsidones Modulate the Nrf2, NF-κB and STAT3 Signaling Pathways in Colorectal Cancer Cells"

    Article Title: Lichen-Derived Depsides and Depsidones Modulate the Nrf2, NF-κB and STAT3 Signaling Pathways in Colorectal Cancer Cells

    Journal: Molecules

    doi: 10.3390/molecules26164787

    The effect of lichen-derived compounds on Nrf2 activation in DLD-1 and HCT116 cells. ( A ) The level of Nrf2 protein in the cytosolic fraction. ( B ) The level of Nrf2 protein in the nuclear fraction. ( C ) The level of p-Nrf2 protein in the nuclear fraction. Representative Western immunoblots are presented under the graphs ( A – C ). Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin or lamin. ( D ) The level of Nrf2 binding to DNA. Results are presented as the means ± SEM from three separate experiments percentage of control). Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).
    Figure Legend Snippet: The effect of lichen-derived compounds on Nrf2 activation in DLD-1 and HCT116 cells. ( A ) The level of Nrf2 protein in the cytosolic fraction. ( B ) The level of Nrf2 protein in the nuclear fraction. ( C ) The level of p-Nrf2 protein in the nuclear fraction. Representative Western immunoblots are presented under the graphs ( A – C ). Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin or lamin. ( D ) The level of Nrf2 binding to DNA. Results are presented as the means ± SEM from three separate experiments percentage of control). Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Techniques Used: Derivative Assay, Activation Assay, Western Blot, Binding Assay

    The effect of lichen-derived compounds on the expression of Nrf2 in DLD-1 and HCT116 cells. The values (fold of control) are presented as the means ± SEM from three separate experiments. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).
    Figure Legend Snippet: The effect of lichen-derived compounds on the expression of Nrf2 in DLD-1 and HCT116 cells. The values (fold of control) are presented as the means ± SEM from three separate experiments. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Techniques Used: Derivative Assay, Expressing

    The effect of lichen-derived compounds on the expression of selected Nrf2 target genes: SOD and GSTP in DLD-1 and HCT116 cells. ( A ) Levels of SOD and GSTP transcripts. The values (fold of control) are presented as the means ± SEM from three separate experiments. ( B ) Level of SOD and GSTP proteins. Representative Western immunoblots are presented under the graphs. Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).
    Figure Legend Snippet: The effect of lichen-derived compounds on the expression of selected Nrf2 target genes: SOD and GSTP in DLD-1 and HCT116 cells. ( A ) Levels of SOD and GSTP transcripts. The values (fold of control) are presented as the means ± SEM from three separate experiments. ( B ) Level of SOD and GSTP proteins. Representative Western immunoblots are presented under the graphs. Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Techniques Used: Derivative Assay, Expressing, Western Blot

    The effect of lichen-derived compounds on the expression of selected Nrf2 target genes: CAT and GPx in DLD-1 and HCT116 cells. ( A ) Levels of CAT and GPx transcripts. The values (fold of control) are presented as the means ± SEM from three separate experiments. ( B ) Levels of CAT and GPx proteins. Representative Western immunoblots are presented under the graphs. Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).
    Figure Legend Snippet: The effect of lichen-derived compounds on the expression of selected Nrf2 target genes: CAT and GPx in DLD-1 and HCT116 cells. ( A ) Levels of CAT and GPx transcripts. The values (fold of control) are presented as the means ± SEM from three separate experiments. ( B ) Levels of CAT and GPx proteins. Representative Western immunoblots are presented under the graphs. Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Techniques Used: Derivative Assay, Expressing, Western Blot

    The effect of lichen-derived compounds on NF-κB activation in DLD-1 and HCT116 cells. ( A ) The levels of NF-κB p50 and p65 proteins in the cytosolic fraction. ( B ) The levels of NF-κB p50 and p65 proteins in the nuclear fraction. Representative Western immunoblots are presented under the graphs ( A , B ). Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin or lamin. ( C ) The levels of NF-κB p50 and p65 binding to DNA. Results are presented as the means ± SEM from three separate experiments percentage of control). Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).
    Figure Legend Snippet: The effect of lichen-derived compounds on NF-κB activation in DLD-1 and HCT116 cells. ( A ) The levels of NF-κB p50 and p65 proteins in the cytosolic fraction. ( B ) The levels of NF-κB p50 and p65 proteins in the nuclear fraction. Representative Western immunoblots are presented under the graphs ( A , B ). Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin or lamin. ( C ) The levels of NF-κB p50 and p65 binding to DNA. Results are presented as the means ± SEM from three separate experiments percentage of control). Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Techniques Used: Derivative Assay, Activation Assay, Western Blot, Binding Assay

    The effect of lichen-derived compounds on the expression of NF-κB p50 and p65 in DLD-1 and HCT116 cells. The values (fold of control) are presented as the means ± SEM from three separate experiments. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).
    Figure Legend Snippet: The effect of lichen-derived compounds on the expression of NF-κB p50 and p65 in DLD-1 and HCT116 cells. The values (fold of control) are presented as the means ± SEM from three separate experiments. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Techniques Used: Derivative Assay, Expressing

    The effect of lichen-derived compounds on the expression of selected NF-κB target genes: COX-2 and iNOS in DLD-1 and HCT116 cells. ( A ) Levels of COX-2 and iNOS transcripts. The values (fold of control) are presented as the means ± SEM from three separate experiments. ( B ) Levels of COX-2 and iNOS proteins. Representative Western immunoblots are presented under the graphs. Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).
    Figure Legend Snippet: The effect of lichen-derived compounds on the expression of selected NF-κB target genes: COX-2 and iNOS in DLD-1 and HCT116 cells. ( A ) Levels of COX-2 and iNOS transcripts. The values (fold of control) are presented as the means ± SEM from three separate experiments. ( B ) Levels of COX-2 and iNOS proteins. Representative Western immunoblots are presented under the graphs. Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Techniques Used: Derivative Assay, Expressing, Western Blot

    The effect of lichen-derived compounds on STAT3 activation in DLD-1 and HCT116 cells. ( A ) The level of STAT3 protein in the cytosolic fraction. ( B ) The level of STAT3 protein in the nuclear fraction. Representative Western immunoblots are presented under the graphs ( A , B ). Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin or lamin. ( C ) The level of STAT3 binding to DNA. Results are presented as the means ± SEM from three separate experiments percentage of control). Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).
    Figure Legend Snippet: The effect of lichen-derived compounds on STAT3 activation in DLD-1 and HCT116 cells. ( A ) The level of STAT3 protein in the cytosolic fraction. ( B ) The level of STAT3 protein in the nuclear fraction. Representative Western immunoblots are presented under the graphs ( A , B ). Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin or lamin. ( C ) The level of STAT3 binding to DNA. Results are presented as the means ± SEM from three separate experiments percentage of control). Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Techniques Used: Derivative Assay, Activation Assay, Western Blot, Binding Assay

    The effect of lichen-derived compounds on the level of p-STAT3 in DLD-1 and HCT116 cells. ( A ) The level of p-STAT3 protein in the nuclear fraction. Representative Western immunoblots are presented under the graphs. Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of lamin. ( B ) The ratio of nuclear p-STAT3 and nuclear STAT3 compared with the control group. DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).
    Figure Legend Snippet: The effect of lichen-derived compounds on the level of p-STAT3 in DLD-1 and HCT116 cells. ( A ) The level of p-STAT3 protein in the nuclear fraction. Representative Western immunoblots are presented under the graphs. Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of lamin. ( B ) The ratio of nuclear p-STAT3 and nuclear STAT3 compared with the control group. DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Techniques Used: Derivative Assay, Western Blot

    The effect of lichen-derived compounds on the expression of STAT3 in DLD-1 and HCT116 cells. The values (fold of control) are presented as the means ± SEM from three separate experiments. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).
    Figure Legend Snippet: The effect of lichen-derived compounds on the expression of STAT3 in DLD-1 and HCT116 cells. The values (fold of control) are presented as the means ± SEM from three separate experiments. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Techniques Used: Derivative Assay, Expressing

    The effect of lichen-derived compounds on the expression of selected STAT3 target gene: Bcl-xl in DLD-1 and HCT116 cells. ( A ) Level of the Bcl-xl transcript. The values (fold of control) are presented as the means ± SEM from three separate experiments. ( B ) Level of the Bcl-xl protein. Representative Western immunoblots are presented under the graphs. Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).
    Figure Legend Snippet: The effect of lichen-derived compounds on the expression of selected STAT3 target gene: Bcl-xl in DLD-1 and HCT116 cells. ( A ) Level of the Bcl-xl transcript. The values (fold of control) are presented as the means ± SEM from three separate experiments. ( B ) Level of the Bcl-xl protein. Representative Western immunoblots are presented under the graphs. Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Techniques Used: Derivative Assay, Expressing, Western Blot

    human colorectal adenocarcinomas  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH human colorectal adenocarcinomas
    Comparing the influence of V s on cell uptake of low and medium clustering MNPs (PVA-coated MNPs vs. CMX-coated MNPs). V s and V d are reported in meter per second (m/s). The uptake amount is reported in picogram iron per cell (pg Fe/cell). Four different cell culture media were used in this study (defined in the material and method section). HT1080, T24, and HT29 cell lines were cultured in culture medium 1, MDA-MB-231 in culture medium 2, BxPC-3 in culture medium 3, and <t> LS174T </t> in culture medium 4.
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    1) Product Images from "Magnetic Nanoparticles Behavior in Biological Solutions; The Impact of Clustering Tendency on Sedimentation Velocity and Cell Uptake"

    Article Title: Magnetic Nanoparticles Behavior in Biological Solutions; The Impact of Clustering Tendency on Sedimentation Velocity and Cell Uptake

    Journal: Materials

    doi: 10.3390/ma13071644

    Comparing the influence of V s on cell uptake of low and medium clustering MNPs (PVA-coated MNPs vs. CMX-coated MNPs). V s and V d are reported in meter per second (m/s). The uptake amount is reported in picogram iron per cell (pg Fe/cell). Four different cell culture media were used in this study (defined in the material and method section). HT1080, T24, and HT29 cell lines were cultured in culture medium 1, MDA-MB-231 in culture medium 2, BxPC-3 in culture medium 3, and  LS174T  in culture medium 4.
    Figure Legend Snippet: Comparing the influence of V s on cell uptake of low and medium clustering MNPs (PVA-coated MNPs vs. CMX-coated MNPs). V s and V d are reported in meter per second (m/s). The uptake amount is reported in picogram iron per cell (pg Fe/cell). Four different cell culture media were used in this study (defined in the material and method section). HT1080, T24, and HT29 cell lines were cultured in culture medium 1, MDA-MB-231 in culture medium 2, BxPC-3 in culture medium 3, and LS174T in culture medium 4.

    Techniques Used: Cell Culture

    human colorectal cell lines sw480  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH human colorectal cell lines sw480
    SLC34A2 induces EZH2 expression and activates EZH2 promoter activity ( A ) Relative expression of SLC34A2 (band size: 76 kDa) in different cell line was examined by qPCR and Western blot. Columns, mean of three individual experiments; S.D., **, P <0.01. All data are representative of at least three independent experiments. ( B ) The proliferation relative gene expression levels are represented in the 3D bar chart. For each gene there are four replicates in this assay. All data are representative of at least three independent experiments. ( C ) Relative expression of SLC34A2 in constructed stable sublines of <t>SW480</t> and HT29 by infection with lenti-si-SLC34A2 or lenti-p-SLC34A2 was examined by qPCR. For each gene, there are six replicated wells in qPCR. Columns, mean of three individual experiments; S.D., **, P <0.01. ( D ) Relative expression of EZH2 was examined by qPCR. For each gene there are six replicated wells in qPCR. Columns, mean of three individual experiments; S.D., **, P <0.01. ( E ) Western blot was used to detect the protein levels of SLC34A2, EZH2 (band size: 85 kDa) and cyclin D1 (band size: 34 kDa) expression in lenti-si-SLC34A2, lenti-si-SLC34A2+ p-SLC34A2 or non-T group. GAPDH was used as a loading control. All data are representative of at least three independent experiments. ( F ) The luciferase activity was determined in lenti-si-SLC34A2, lenti-si-NC, and non-T group. There are six replicated wells for each group in this assay. Columns, mean of three individual experiments; S.D., **, P <0.01. Abbreviations: lenti, lentivirus mediated infection; non-T, non-treated; p, pcDNA3.1.
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    1) Product Images from "The SLC34A2-ROS-HIF-1-induced up-regulation of EZH2 expression promotes proliferation and chemo-resistance to apoptosis in colorectal cancer"

    Article Title: The SLC34A2-ROS-HIF-1-induced up-regulation of EZH2 expression promotes proliferation and chemo-resistance to apoptosis in colorectal cancer

    Journal: Bioscience Reports

    doi: 10.1042/BSR20180268

    SLC34A2 induces EZH2 expression and activates EZH2 promoter activity ( A ) Relative expression of SLC34A2 (band size: 76 kDa) in different cell line was examined by qPCR and Western blot. Columns, mean of three individual experiments; S.D., **, P <0.01. All data are representative of at least three independent experiments. ( B ) The proliferation relative gene expression levels are represented in the 3D bar chart. For each gene there are four replicates in this assay. All data are representative of at least three independent experiments. ( C ) Relative expression of SLC34A2 in constructed stable sublines of SW480 and HT29 by infection with lenti-si-SLC34A2 or lenti-p-SLC34A2 was examined by qPCR. For each gene, there are six replicated wells in qPCR. Columns, mean of three individual experiments; S.D., **, P <0.01. ( D ) Relative expression of EZH2 was examined by qPCR. For each gene there are six replicated wells in qPCR. Columns, mean of three individual experiments; S.D., **, P <0.01. ( E ) Western blot was used to detect the protein levels of SLC34A2, EZH2 (band size: 85 kDa) and cyclin D1 (band size: 34 kDa) expression in lenti-si-SLC34A2, lenti-si-SLC34A2+ p-SLC34A2 or non-T group. GAPDH was used as a loading control. All data are representative of at least three independent experiments. ( F ) The luciferase activity was determined in lenti-si-SLC34A2, lenti-si-NC, and non-T group. There are six replicated wells for each group in this assay. Columns, mean of three individual experiments; S.D., **, P <0.01. Abbreviations: lenti, lentivirus mediated infection; non-T, non-treated; p, pcDNA3.1.
    Figure Legend Snippet: SLC34A2 induces EZH2 expression and activates EZH2 promoter activity ( A ) Relative expression of SLC34A2 (band size: 76 kDa) in different cell line was examined by qPCR and Western blot. Columns, mean of three individual experiments; S.D., **, P <0.01. All data are representative of at least three independent experiments. ( B ) The proliferation relative gene expression levels are represented in the 3D bar chart. For each gene there are four replicates in this assay. All data are representative of at least three independent experiments. ( C ) Relative expression of SLC34A2 in constructed stable sublines of SW480 and HT29 by infection with lenti-si-SLC34A2 or lenti-p-SLC34A2 was examined by qPCR. For each gene, there are six replicated wells in qPCR. Columns, mean of three individual experiments; S.D., **, P <0.01. ( D ) Relative expression of EZH2 was examined by qPCR. For each gene there are six replicated wells in qPCR. Columns, mean of three individual experiments; S.D., **, P <0.01. ( E ) Western blot was used to detect the protein levels of SLC34A2, EZH2 (band size: 85 kDa) and cyclin D1 (band size: 34 kDa) expression in lenti-si-SLC34A2, lenti-si-SLC34A2+ p-SLC34A2 or non-T group. GAPDH was used as a loading control. All data are representative of at least three independent experiments. ( F ) The luciferase activity was determined in lenti-si-SLC34A2, lenti-si-NC, and non-T group. There are six replicated wells for each group in this assay. Columns, mean of three individual experiments; S.D., **, P <0.01. Abbreviations: lenti, lentivirus mediated infection; non-T, non-treated; p, pcDNA3.1.

    Techniques Used: Expressing, Activity Assay, Western Blot, Construct, Infection, Luciferase

    human colorectal adenocarcinoma cell lines  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH human colorectal adenocarcinoma cell lines
    Human Colorectal Adenocarcinoma Cell Lines, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human colorectal adenocarcinoma cells  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH human colorectal adenocarcinoma cells
    Human Colorectal Adenocarcinoma Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    CLS Cell Lines Service GmbH human colorectal
    The effect of lichen-derived compounds on Nrf2 activation in DLD-1 and <t>HCT116</t> cells. ( A ) The level of Nrf2 protein in the cytosolic fraction. ( B ) The level of Nrf2 protein in the nuclear fraction. ( C ) The level of p-Nrf2 protein in the nuclear fraction. Representative Western immunoblots are presented under the graphs ( A – C ). Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin or lamin. ( D ) The level of Nrf2 binding to DNA. Results are presented as the means ± SEM from three separate experiments percentage of control). Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).
    Human Colorectal, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    CLS Cell Lines Service GmbH human epithelial colorectal adenocarcinoma cells
    The effect of lichen-derived compounds on Nrf2 activation in DLD-1 and <t>HCT116</t> cells. ( A ) The level of Nrf2 protein in the cytosolic fraction. ( B ) The level of Nrf2 protein in the nuclear fraction. ( C ) The level of p-Nrf2 protein in the nuclear fraction. Representative Western immunoblots are presented under the graphs ( A – C ). Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin or lamin. ( D ) The level of Nrf2 binding to DNA. Results are presented as the means ± SEM from three separate experiments percentage of control). Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).
    Human Epithelial Colorectal Adenocarcinoma Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    CLS Cell Lines Service GmbH human colorectal cancer cell line ht
    (A) Measuring cell viability following short-term hyperthermia. In vitro exposure of colorectal cancer cells <t>(HT-29)</t> to short-term hyperthermia (10 sec). Exposure to temperature levels from 37˚C (control) in 5˚C step increments until 80˚C. An additional control with Oxa. At 37˚C was conducted to compare vitality levels with chemotherapy. (B) Measuring cytotoxicity following hyperthermia: In vitro exposure of colorectal cancer (HT-29) cells to short-term hyperthermia (10 sec). Exposure to temperature levels of 37˚C (Control) in 5˚C step increments until 80˚C. An additional control with Oxa. At 37˚C was conducted to help compare vitality levels with chemotherapy. # P>0.05, * P<0.05, ** P<0.01. Oxa., oxaliplatin; LDH, lactate dehydrogenase.
    Human Colorectal Cancer Cell Line Ht, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    CLS Cell Lines Service GmbH human colorectal carcinoma cells
    <t>Colorectal</t> cancer <t>cells—HCT</t> 116 nuclei stained with Hoechst 33342 dye after a 24 h treatment with Mentha piperita L. essential oil—M_EO (10 and 150 μg/mL). Staurosporine (5 µM) was used as the positive control for apoptotic changes at nuclear level and Triton X-100 (0.5%) for necrosis. The yellow arrows indicate signs of apoptosis, and the red arrow shows signs of necrosis. The scale bar was 20 µm.
    Human Colorectal Carcinoma Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human colorectal carcinoma cells - by Bioz Stars, 2024-05
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    86
    CLS Cell Lines Service GmbH human colorectal adenocarcinoma cell line hct15
    <t>Colorectal</t> cancer <t>cells—HCT</t> 116 nuclei stained with Hoechst 33342 dye after a 24 h treatment with Mentha piperita L. essential oil—M_EO (10 and 150 μg/mL). Staurosporine (5 µM) was used as the positive control for apoptotic changes at nuclear level and Triton X-100 (0.5%) for necrosis. The yellow arrows indicate signs of apoptosis, and the red arrow shows signs of necrosis. The scale bar was 20 µm.
    Human Colorectal Adenocarcinoma Cell Line Hct15, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    CLS Cell Lines Service GmbH human colorectal adenocarcinomas
    Comparing the influence of V s on cell uptake of low and medium clustering MNPs (PVA-coated MNPs vs. CMX-coated MNPs). V s and V d are reported in meter per second (m/s). The uptake amount is reported in picogram iron per cell (pg Fe/cell). Four different cell culture media were used in this study (defined in the material and method section). HT1080, T24, and HT29 cell lines were cultured in culture medium 1, MDA-MB-231 in culture medium 2, BxPC-3 in culture medium 3, and <t> LS174T </t> in culture medium 4.
    Human Colorectal Adenocarcinomas, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    CLS Cell Lines Service GmbH human colorectal cell lines sw480
    SLC34A2 induces EZH2 expression and activates EZH2 promoter activity ( A ) Relative expression of SLC34A2 (band size: 76 kDa) in different cell line was examined by qPCR and Western blot. Columns, mean of three individual experiments; S.D., **, P <0.01. All data are representative of at least three independent experiments. ( B ) The proliferation relative gene expression levels are represented in the 3D bar chart. For each gene there are four replicates in this assay. All data are representative of at least three independent experiments. ( C ) Relative expression of SLC34A2 in constructed stable sublines of <t>SW480</t> and HT29 by infection with lenti-si-SLC34A2 or lenti-p-SLC34A2 was examined by qPCR. For each gene, there are six replicated wells in qPCR. Columns, mean of three individual experiments; S.D., **, P <0.01. ( D ) Relative expression of EZH2 was examined by qPCR. For each gene there are six replicated wells in qPCR. Columns, mean of three individual experiments; S.D., **, P <0.01. ( E ) Western blot was used to detect the protein levels of SLC34A2, EZH2 (band size: 85 kDa) and cyclin D1 (band size: 34 kDa) expression in lenti-si-SLC34A2, lenti-si-SLC34A2+ p-SLC34A2 or non-T group. GAPDH was used as a loading control. All data are representative of at least three independent experiments. ( F ) The luciferase activity was determined in lenti-si-SLC34A2, lenti-si-NC, and non-T group. There are six replicated wells for each group in this assay. Columns, mean of three individual experiments; S.D., **, P <0.01. Abbreviations: lenti, lentivirus mediated infection; non-T, non-treated; p, pcDNA3.1.
    Human Colorectal Cell Lines Sw480, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human colorectal cell lines sw480 - by Bioz Stars, 2024-05
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    86
    CLS Cell Lines Service GmbH human colorectal adenocarcinoma cell lines
    SLC34A2 induces EZH2 expression and activates EZH2 promoter activity ( A ) Relative expression of SLC34A2 (band size: 76 kDa) in different cell line was examined by qPCR and Western blot. Columns, mean of three individual experiments; S.D., **, P <0.01. All data are representative of at least three independent experiments. ( B ) The proliferation relative gene expression levels are represented in the 3D bar chart. For each gene there are four replicates in this assay. All data are representative of at least three independent experiments. ( C ) Relative expression of SLC34A2 in constructed stable sublines of <t>SW480</t> and HT29 by infection with lenti-si-SLC34A2 or lenti-p-SLC34A2 was examined by qPCR. For each gene, there are six replicated wells in qPCR. Columns, mean of three individual experiments; S.D., **, P <0.01. ( D ) Relative expression of EZH2 was examined by qPCR. For each gene there are six replicated wells in qPCR. Columns, mean of three individual experiments; S.D., **, P <0.01. ( E ) Western blot was used to detect the protein levels of SLC34A2, EZH2 (band size: 85 kDa) and cyclin D1 (band size: 34 kDa) expression in lenti-si-SLC34A2, lenti-si-SLC34A2+ p-SLC34A2 or non-T group. GAPDH was used as a loading control. All data are representative of at least three independent experiments. ( F ) The luciferase activity was determined in lenti-si-SLC34A2, lenti-si-NC, and non-T group. There are six replicated wells for each group in this assay. Columns, mean of three individual experiments; S.D., **, P <0.01. Abbreviations: lenti, lentivirus mediated infection; non-T, non-treated; p, pcDNA3.1.
    Human Colorectal Adenocarcinoma Cell Lines, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human colorectal adenocarcinoma cell lines/product/CLS Cell Lines Service GmbH
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    human colorectal adenocarcinoma cell lines - by Bioz Stars, 2024-05
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    86
    CLS Cell Lines Service GmbH human colorectal adenocarcinoma cells
    SLC34A2 induces EZH2 expression and activates EZH2 promoter activity ( A ) Relative expression of SLC34A2 (band size: 76 kDa) in different cell line was examined by qPCR and Western blot. Columns, mean of three individual experiments; S.D., **, P <0.01. All data are representative of at least three independent experiments. ( B ) The proliferation relative gene expression levels are represented in the 3D bar chart. For each gene there are four replicates in this assay. All data are representative of at least three independent experiments. ( C ) Relative expression of SLC34A2 in constructed stable sublines of <t>SW480</t> and HT29 by infection with lenti-si-SLC34A2 or lenti-p-SLC34A2 was examined by qPCR. For each gene, there are six replicated wells in qPCR. Columns, mean of three individual experiments; S.D., **, P <0.01. ( D ) Relative expression of EZH2 was examined by qPCR. For each gene there are six replicated wells in qPCR. Columns, mean of three individual experiments; S.D., **, P <0.01. ( E ) Western blot was used to detect the protein levels of SLC34A2, EZH2 (band size: 85 kDa) and cyclin D1 (band size: 34 kDa) expression in lenti-si-SLC34A2, lenti-si-SLC34A2+ p-SLC34A2 or non-T group. GAPDH was used as a loading control. All data are representative of at least three independent experiments. ( F ) The luciferase activity was determined in lenti-si-SLC34A2, lenti-si-NC, and non-T group. There are six replicated wells for each group in this assay. Columns, mean of three individual experiments; S.D., **, P <0.01. Abbreviations: lenti, lentivirus mediated infection; non-T, non-treated; p, pcDNA3.1.
    Human Colorectal Adenocarcinoma Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human colorectal adenocarcinoma cells/product/CLS Cell Lines Service GmbH
    Average 86 stars, based on 1 article reviews
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    human colorectal adenocarcinoma cells - by Bioz Stars, 2024-05
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    Image Search Results


    The effect of lichen-derived compounds on Nrf2 activation in DLD-1 and HCT116 cells. ( A ) The level of Nrf2 protein in the cytosolic fraction. ( B ) The level of Nrf2 protein in the nuclear fraction. ( C ) The level of p-Nrf2 protein in the nuclear fraction. Representative Western immunoblots are presented under the graphs ( A – C ). Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin or lamin. ( D ) The level of Nrf2 binding to DNA. Results are presented as the means ± SEM from three separate experiments percentage of control). Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Journal: Molecules

    Article Title: Lichen-Derived Depsides and Depsidones Modulate the Nrf2, NF-κB and STAT3 Signaling Pathways in Colorectal Cancer Cells

    doi: 10.3390/molecules26164787

    Figure Lengend Snippet: The effect of lichen-derived compounds on Nrf2 activation in DLD-1 and HCT116 cells. ( A ) The level of Nrf2 protein in the cytosolic fraction. ( B ) The level of Nrf2 protein in the nuclear fraction. ( C ) The level of p-Nrf2 protein in the nuclear fraction. Representative Western immunoblots are presented under the graphs ( A – C ). Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin or lamin. ( D ) The level of Nrf2 binding to DNA. Results are presented as the means ± SEM from three separate experiments percentage of control). Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Article Snippet: Human colorectal (HCT116 and DLD-1) cancer cells were obtained from the European Collection of Authenticated Cell Culture (Cell Lines Service, Eppelheim, Germany).

    Techniques: Derivative Assay, Activation Assay, Western Blot, Binding Assay

    The effect of lichen-derived compounds on the expression of Nrf2 in DLD-1 and HCT116 cells. The values (fold of control) are presented as the means ± SEM from three separate experiments. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Journal: Molecules

    Article Title: Lichen-Derived Depsides and Depsidones Modulate the Nrf2, NF-κB and STAT3 Signaling Pathways in Colorectal Cancer Cells

    doi: 10.3390/molecules26164787

    Figure Lengend Snippet: The effect of lichen-derived compounds on the expression of Nrf2 in DLD-1 and HCT116 cells. The values (fold of control) are presented as the means ± SEM from three separate experiments. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Article Snippet: Human colorectal (HCT116 and DLD-1) cancer cells were obtained from the European Collection of Authenticated Cell Culture (Cell Lines Service, Eppelheim, Germany).

    Techniques: Derivative Assay, Expressing

    The effect of lichen-derived compounds on the expression of selected Nrf2 target genes: SOD and GSTP in DLD-1 and HCT116 cells. ( A ) Levels of SOD and GSTP transcripts. The values (fold of control) are presented as the means ± SEM from three separate experiments. ( B ) Level of SOD and GSTP proteins. Representative Western immunoblots are presented under the graphs. Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Journal: Molecules

    Article Title: Lichen-Derived Depsides and Depsidones Modulate the Nrf2, NF-κB and STAT3 Signaling Pathways in Colorectal Cancer Cells

    doi: 10.3390/molecules26164787

    Figure Lengend Snippet: The effect of lichen-derived compounds on the expression of selected Nrf2 target genes: SOD and GSTP in DLD-1 and HCT116 cells. ( A ) Levels of SOD and GSTP transcripts. The values (fold of control) are presented as the means ± SEM from three separate experiments. ( B ) Level of SOD and GSTP proteins. Representative Western immunoblots are presented under the graphs. Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Article Snippet: Human colorectal (HCT116 and DLD-1) cancer cells were obtained from the European Collection of Authenticated Cell Culture (Cell Lines Service, Eppelheim, Germany).

    Techniques: Derivative Assay, Expressing, Western Blot

    The effect of lichen-derived compounds on the expression of selected Nrf2 target genes: CAT and GPx in DLD-1 and HCT116 cells. ( A ) Levels of CAT and GPx transcripts. The values (fold of control) are presented as the means ± SEM from three separate experiments. ( B ) Levels of CAT and GPx proteins. Representative Western immunoblots are presented under the graphs. Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Journal: Molecules

    Article Title: Lichen-Derived Depsides and Depsidones Modulate the Nrf2, NF-κB and STAT3 Signaling Pathways in Colorectal Cancer Cells

    doi: 10.3390/molecules26164787

    Figure Lengend Snippet: The effect of lichen-derived compounds on the expression of selected Nrf2 target genes: CAT and GPx in DLD-1 and HCT116 cells. ( A ) Levels of CAT and GPx transcripts. The values (fold of control) are presented as the means ± SEM from three separate experiments. ( B ) Levels of CAT and GPx proteins. Representative Western immunoblots are presented under the graphs. Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Article Snippet: Human colorectal (HCT116 and DLD-1) cancer cells were obtained from the European Collection of Authenticated Cell Culture (Cell Lines Service, Eppelheim, Germany).

    Techniques: Derivative Assay, Expressing, Western Blot

    The effect of lichen-derived compounds on NF-κB activation in DLD-1 and HCT116 cells. ( A ) The levels of NF-κB p50 and p65 proteins in the cytosolic fraction. ( B ) The levels of NF-κB p50 and p65 proteins in the nuclear fraction. Representative Western immunoblots are presented under the graphs ( A , B ). Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin or lamin. ( C ) The levels of NF-κB p50 and p65 binding to DNA. Results are presented as the means ± SEM from three separate experiments percentage of control). Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Journal: Molecules

    Article Title: Lichen-Derived Depsides and Depsidones Modulate the Nrf2, NF-κB and STAT3 Signaling Pathways in Colorectal Cancer Cells

    doi: 10.3390/molecules26164787

    Figure Lengend Snippet: The effect of lichen-derived compounds on NF-κB activation in DLD-1 and HCT116 cells. ( A ) The levels of NF-κB p50 and p65 proteins in the cytosolic fraction. ( B ) The levels of NF-κB p50 and p65 proteins in the nuclear fraction. Representative Western immunoblots are presented under the graphs ( A , B ). Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin or lamin. ( C ) The levels of NF-κB p50 and p65 binding to DNA. Results are presented as the means ± SEM from three separate experiments percentage of control). Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Article Snippet: Human colorectal (HCT116 and DLD-1) cancer cells were obtained from the European Collection of Authenticated Cell Culture (Cell Lines Service, Eppelheim, Germany).

    Techniques: Derivative Assay, Activation Assay, Western Blot, Binding Assay

    The effect of lichen-derived compounds on the expression of NF-κB p50 and p65 in DLD-1 and HCT116 cells. The values (fold of control) are presented as the means ± SEM from three separate experiments. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Journal: Molecules

    Article Title: Lichen-Derived Depsides and Depsidones Modulate the Nrf2, NF-κB and STAT3 Signaling Pathways in Colorectal Cancer Cells

    doi: 10.3390/molecules26164787

    Figure Lengend Snippet: The effect of lichen-derived compounds on the expression of NF-κB p50 and p65 in DLD-1 and HCT116 cells. The values (fold of control) are presented as the means ± SEM from three separate experiments. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Article Snippet: Human colorectal (HCT116 and DLD-1) cancer cells were obtained from the European Collection of Authenticated Cell Culture (Cell Lines Service, Eppelheim, Germany).

    Techniques: Derivative Assay, Expressing

    The effect of lichen-derived compounds on the expression of selected NF-κB target genes: COX-2 and iNOS in DLD-1 and HCT116 cells. ( A ) Levels of COX-2 and iNOS transcripts. The values (fold of control) are presented as the means ± SEM from three separate experiments. ( B ) Levels of COX-2 and iNOS proteins. Representative Western immunoblots are presented under the graphs. Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Journal: Molecules

    Article Title: Lichen-Derived Depsides and Depsidones Modulate the Nrf2, NF-κB and STAT3 Signaling Pathways in Colorectal Cancer Cells

    doi: 10.3390/molecules26164787

    Figure Lengend Snippet: The effect of lichen-derived compounds on the expression of selected NF-κB target genes: COX-2 and iNOS in DLD-1 and HCT116 cells. ( A ) Levels of COX-2 and iNOS transcripts. The values (fold of control) are presented as the means ± SEM from three separate experiments. ( B ) Levels of COX-2 and iNOS proteins. Representative Western immunoblots are presented under the graphs. Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Article Snippet: Human colorectal (HCT116 and DLD-1) cancer cells were obtained from the European Collection of Authenticated Cell Culture (Cell Lines Service, Eppelheim, Germany).

    Techniques: Derivative Assay, Expressing, Western Blot

    The effect of lichen-derived compounds on STAT3 activation in DLD-1 and HCT116 cells. ( A ) The level of STAT3 protein in the cytosolic fraction. ( B ) The level of STAT3 protein in the nuclear fraction. Representative Western immunoblots are presented under the graphs ( A , B ). Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin or lamin. ( C ) The level of STAT3 binding to DNA. Results are presented as the means ± SEM from three separate experiments percentage of control). Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Journal: Molecules

    Article Title: Lichen-Derived Depsides and Depsidones Modulate the Nrf2, NF-κB and STAT3 Signaling Pathways in Colorectal Cancer Cells

    doi: 10.3390/molecules26164787

    Figure Lengend Snippet: The effect of lichen-derived compounds on STAT3 activation in DLD-1 and HCT116 cells. ( A ) The level of STAT3 protein in the cytosolic fraction. ( B ) The level of STAT3 protein in the nuclear fraction. Representative Western immunoblots are presented under the graphs ( A , B ). Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin or lamin. ( C ) The level of STAT3 binding to DNA. Results are presented as the means ± SEM from three separate experiments percentage of control). Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Article Snippet: Human colorectal (HCT116 and DLD-1) cancer cells were obtained from the European Collection of Authenticated Cell Culture (Cell Lines Service, Eppelheim, Germany).

    Techniques: Derivative Assay, Activation Assay, Western Blot, Binding Assay

    The effect of lichen-derived compounds on the level of p-STAT3 in DLD-1 and HCT116 cells. ( A ) The level of p-STAT3 protein in the nuclear fraction. Representative Western immunoblots are presented under the graphs. Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of lamin. ( B ) The ratio of nuclear p-STAT3 and nuclear STAT3 compared with the control group. DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Journal: Molecules

    Article Title: Lichen-Derived Depsides and Depsidones Modulate the Nrf2, NF-κB and STAT3 Signaling Pathways in Colorectal Cancer Cells

    doi: 10.3390/molecules26164787

    Figure Lengend Snippet: The effect of lichen-derived compounds on the level of p-STAT3 in DLD-1 and HCT116 cells. ( A ) The level of p-STAT3 protein in the nuclear fraction. Representative Western immunoblots are presented under the graphs. Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of lamin. ( B ) The ratio of nuclear p-STAT3 and nuclear STAT3 compared with the control group. DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Article Snippet: Human colorectal (HCT116 and DLD-1) cancer cells were obtained from the European Collection of Authenticated Cell Culture (Cell Lines Service, Eppelheim, Germany).

    Techniques: Derivative Assay, Western Blot

    The effect of lichen-derived compounds on the expression of STAT3 in DLD-1 and HCT116 cells. The values (fold of control) are presented as the means ± SEM from three separate experiments. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Journal: Molecules

    Article Title: Lichen-Derived Depsides and Depsidones Modulate the Nrf2, NF-κB and STAT3 Signaling Pathways in Colorectal Cancer Cells

    doi: 10.3390/molecules26164787

    Figure Lengend Snippet: The effect of lichen-derived compounds on the expression of STAT3 in DLD-1 and HCT116 cells. The values (fold of control) are presented as the means ± SEM from three separate experiments. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Article Snippet: Human colorectal (HCT116 and DLD-1) cancer cells were obtained from the European Collection of Authenticated Cell Culture (Cell Lines Service, Eppelheim, Germany).

    Techniques: Derivative Assay, Expressing

    The effect of lichen-derived compounds on the expression of selected STAT3 target gene: Bcl-xl in DLD-1 and HCT116 cells. ( A ) Level of the Bcl-xl transcript. The values (fold of control) are presented as the means ± SEM from three separate experiments. ( B ) Level of the Bcl-xl protein. Representative Western immunoblots are presented under the graphs. Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Journal: Molecules

    Article Title: Lichen-Derived Depsides and Depsidones Modulate the Nrf2, NF-κB and STAT3 Signaling Pathways in Colorectal Cancer Cells

    doi: 10.3390/molecules26164787

    Figure Lengend Snippet: The effect of lichen-derived compounds on the expression of selected STAT3 target gene: Bcl-xl in DLD-1 and HCT116 cells. ( A ) Level of the Bcl-xl transcript. The values (fold of control) are presented as the means ± SEM from three separate experiments. ( B ) Level of the Bcl-xl protein. Representative Western immunoblots are presented under the graphs. Results (means ± SEM from three separate experiments) are presented as a fold change to control after normalization against the level of actin. Asterisks (*) denote statistically significant changes from the control group ( p ≤ 0.05). DMSO , vehicle control; Cap50 , caperatic acid (50 µM); Atra50 , atranorin (50 µM); Leca50 , lecanoric acid (50 µM); Squam50 , squamatic acid (50 µM); Phys25 , physodic acid (25 µM); Salaz50 , salazinic acid (50 µM).

    Article Snippet: Human colorectal (HCT116 and DLD-1) cancer cells were obtained from the European Collection of Authenticated Cell Culture (Cell Lines Service, Eppelheim, Germany).

    Techniques: Derivative Assay, Expressing, Western Blot

    (A) Measuring cell viability following short-term hyperthermia. In vitro exposure of colorectal cancer cells (HT-29) to short-term hyperthermia (10 sec). Exposure to temperature levels from 37˚C (control) in 5˚C step increments until 80˚C. An additional control with Oxa. At 37˚C was conducted to compare vitality levels with chemotherapy. (B) Measuring cytotoxicity following hyperthermia: In vitro exposure of colorectal cancer (HT-29) cells to short-term hyperthermia (10 sec). Exposure to temperature levels of 37˚C (Control) in 5˚C step increments until 80˚C. An additional control with Oxa. At 37˚C was conducted to help compare vitality levels with chemotherapy. # P>0.05, * P<0.05, ** P<0.01. Oxa., oxaliplatin; LDH, lactate dehydrogenase.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Evaluating the concept of gas‑based intraperitoneal hyperthermia beyond 43˚C in the treatment of peritoneal metastasis: A pilot study

    doi: 10.3892/etm.2022.11687

    Figure Lengend Snippet: (A) Measuring cell viability following short-term hyperthermia. In vitro exposure of colorectal cancer cells (HT-29) to short-term hyperthermia (10 sec). Exposure to temperature levels from 37˚C (control) in 5˚C step increments until 80˚C. An additional control with Oxa. At 37˚C was conducted to compare vitality levels with chemotherapy. (B) Measuring cytotoxicity following hyperthermia: In vitro exposure of colorectal cancer (HT-29) cells to short-term hyperthermia (10 sec). Exposure to temperature levels of 37˚C (Control) in 5˚C step increments until 80˚C. An additional control with Oxa. At 37˚C was conducted to help compare vitality levels with chemotherapy. # P>0.05, * P<0.05, ** P<0.01. Oxa., oxaliplatin; LDH, lactate dehydrogenase.

    Article Snippet: Human colorectal cancer cell line HT-29 was obtained from CLS (Cell Lines Service GmbH, Eppelheim, Germany).

    Techniques: In Vitro

    Colorectal cancer cells—HCT 116 nuclei stained with Hoechst 33342 dye after a 24 h treatment with Mentha piperita L. essential oil—M_EO (10 and 150 μg/mL). Staurosporine (5 µM) was used as the positive control for apoptotic changes at nuclear level and Triton X-100 (0.5%) for necrosis. The yellow arrows indicate signs of apoptosis, and the red arrow shows signs of necrosis. The scale bar was 20 µm.

    Journal: Molecules

    Article Title: Chemical and Antimicrobial Characterization of Mentha piperita L. and Rosmarinus officinalis L. Essential Oils and In Vitro Potential Cytotoxic Effect in Human Colorectal Carcinoma Cells

    doi: 10.3390/molecules27186106

    Figure Lengend Snippet: Colorectal cancer cells—HCT 116 nuclei stained with Hoechst 33342 dye after a 24 h treatment with Mentha piperita L. essential oil—M_EO (10 and 150 μg/mL). Staurosporine (5 µM) was used as the positive control for apoptotic changes at nuclear level and Triton X-100 (0.5%) for necrosis. The yellow arrows indicate signs of apoptosis, and the red arrow shows signs of necrosis. The scale bar was 20 µm.

    Article Snippet: Two cell lines acquired from ATCC (American Type Cell Collection) and CLS Cell Lines Service GmbH (Eppelheim, Germany), respectively, were used as in vitro models, namely: human colorectal carcinoma cells—HCT 116 (ATCC ® CCL-247TM) and immortalized human keratinocytes—HaCaT.

    Techniques: Staining, Positive Control

    Colorectal cancer cells—HCT 116 nuclei stained with Hoechst 33342 dye after a 24 h treatment with Rosmarinus officinalis L. essential oil—R_EO (10 and 150 μg/mL). Staurosporine (5 µM) was used as the positive control for apoptotic changes at nuclear level and Triton X-100 (0.5%) for necrosis. The yellow arrows indicate signs of apoptosis, and the red arrow shows signs of necrosis. The scale bar was 20 µm.

    Journal: Molecules

    Article Title: Chemical and Antimicrobial Characterization of Mentha piperita L. and Rosmarinus officinalis L. Essential Oils and In Vitro Potential Cytotoxic Effect in Human Colorectal Carcinoma Cells

    doi: 10.3390/molecules27186106

    Figure Lengend Snippet: Colorectal cancer cells—HCT 116 nuclei stained with Hoechst 33342 dye after a 24 h treatment with Rosmarinus officinalis L. essential oil—R_EO (10 and 150 μg/mL). Staurosporine (5 µM) was used as the positive control for apoptotic changes at nuclear level and Triton X-100 (0.5%) for necrosis. The yellow arrows indicate signs of apoptosis, and the red arrow shows signs of necrosis. The scale bar was 20 µm.

    Article Snippet: Two cell lines acquired from ATCC (American Type Cell Collection) and CLS Cell Lines Service GmbH (Eppelheim, Germany), respectively, were used as in vitro models, namely: human colorectal carcinoma cells—HCT 116 (ATCC ® CCL-247TM) and immortalized human keratinocytes—HaCaT.

    Techniques: Staining, Positive Control

    Comparing the influence of V s on cell uptake of low and medium clustering MNPs (PVA-coated MNPs vs. CMX-coated MNPs). V s and V d are reported in meter per second (m/s). The uptake amount is reported in picogram iron per cell (pg Fe/cell). Four different cell culture media were used in this study (defined in the material and method section). HT1080, T24, and HT29 cell lines were cultured in culture medium 1, MDA-MB-231 in culture medium 2, BxPC-3 in culture medium 3, and  LS174T  in culture medium 4.

    Journal: Materials

    Article Title: Magnetic Nanoparticles Behavior in Biological Solutions; The Impact of Clustering Tendency on Sedimentation Velocity and Cell Uptake

    doi: 10.3390/ma13071644

    Figure Lengend Snippet: Comparing the influence of V s on cell uptake of low and medium clustering MNPs (PVA-coated MNPs vs. CMX-coated MNPs). V s and V d are reported in meter per second (m/s). The uptake amount is reported in picogram iron per cell (pg Fe/cell). Four different cell culture media were used in this study (defined in the material and method section). HT1080, T24, and HT29 cell lines were cultured in culture medium 1, MDA-MB-231 in culture medium 2, BxPC-3 in culture medium 3, and LS174T in culture medium 4.

    Article Snippet: Human colorectal adenocarcinomas (LS174T, CLS GmbH, Eppelheim, Germany) cells were grown in MEM with 10% FBS, 1 mM sodium-pyruvate, and 0.1 mM non-essential amino acid (NEAA).

    Techniques: Cell Culture

    SLC34A2 induces EZH2 expression and activates EZH2 promoter activity ( A ) Relative expression of SLC34A2 (band size: 76 kDa) in different cell line was examined by qPCR and Western blot. Columns, mean of three individual experiments; S.D., **, P <0.01. All data are representative of at least three independent experiments. ( B ) The proliferation relative gene expression levels are represented in the 3D bar chart. For each gene there are four replicates in this assay. All data are representative of at least three independent experiments. ( C ) Relative expression of SLC34A2 in constructed stable sublines of SW480 and HT29 by infection with lenti-si-SLC34A2 or lenti-p-SLC34A2 was examined by qPCR. For each gene, there are six replicated wells in qPCR. Columns, mean of three individual experiments; S.D., **, P <0.01. ( D ) Relative expression of EZH2 was examined by qPCR. For each gene there are six replicated wells in qPCR. Columns, mean of three individual experiments; S.D., **, P <0.01. ( E ) Western blot was used to detect the protein levels of SLC34A2, EZH2 (band size: 85 kDa) and cyclin D1 (band size: 34 kDa) expression in lenti-si-SLC34A2, lenti-si-SLC34A2+ p-SLC34A2 or non-T group. GAPDH was used as a loading control. All data are representative of at least three independent experiments. ( F ) The luciferase activity was determined in lenti-si-SLC34A2, lenti-si-NC, and non-T group. There are six replicated wells for each group in this assay. Columns, mean of three individual experiments; S.D., **, P <0.01. Abbreviations: lenti, lentivirus mediated infection; non-T, non-treated; p, pcDNA3.1.

    Journal: Bioscience Reports

    Article Title: The SLC34A2-ROS-HIF-1-induced up-regulation of EZH2 expression promotes proliferation and chemo-resistance to apoptosis in colorectal cancer

    doi: 10.1042/BSR20180268

    Figure Lengend Snippet: SLC34A2 induces EZH2 expression and activates EZH2 promoter activity ( A ) Relative expression of SLC34A2 (band size: 76 kDa) in different cell line was examined by qPCR and Western blot. Columns, mean of three individual experiments; S.D., **, P <0.01. All data are representative of at least three independent experiments. ( B ) The proliferation relative gene expression levels are represented in the 3D bar chart. For each gene there are four replicates in this assay. All data are representative of at least three independent experiments. ( C ) Relative expression of SLC34A2 in constructed stable sublines of SW480 and HT29 by infection with lenti-si-SLC34A2 or lenti-p-SLC34A2 was examined by qPCR. For each gene, there are six replicated wells in qPCR. Columns, mean of three individual experiments; S.D., **, P <0.01. ( D ) Relative expression of EZH2 was examined by qPCR. For each gene there are six replicated wells in qPCR. Columns, mean of three individual experiments; S.D., **, P <0.01. ( E ) Western blot was used to detect the protein levels of SLC34A2, EZH2 (band size: 85 kDa) and cyclin D1 (band size: 34 kDa) expression in lenti-si-SLC34A2, lenti-si-SLC34A2+ p-SLC34A2 or non-T group. GAPDH was used as a loading control. All data are representative of at least three independent experiments. ( F ) The luciferase activity was determined in lenti-si-SLC34A2, lenti-si-NC, and non-T group. There are six replicated wells for each group in this assay. Columns, mean of three individual experiments; S.D., **, P <0.01. Abbreviations: lenti, lentivirus mediated infection; non-T, non-treated; p, pcDNA3.1.

    Article Snippet: Human colorectal cell lines SW480 and HT29 were purchased from CLS Cell Lines Service GmbH (Eppelheim, Germany).

    Techniques: Expressing, Activity Assay, Western Blot, Construct, Infection, Luciferase