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anti human c5b c9 antibody  (Hycult Biotech)


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    Hycult Biotech anti human c5b c9 antibody
    Anti Human C5b C9 Antibody, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti human c5b c9 antibody/product/Hycult Biotech
    Average 91 stars, based on 1 article reviews
    anti human c5b c9 antibody - by Bioz Stars, 2025-03
    91/100 stars

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    Image Search Results


    Deposition of C5b on bacteria. a C5b deposition on selected isolates by titration of NHS (0–10%) by flow cytometry. b C5b deposition on the bacterial surface (polyclonal antibody detecting highlighted α′ chain and β chain; reducing conditions) with the Western blot quantification in c . d C5b deposition on the bacterial surface (antibody detecting cleavage neoepitope in C5b α′ chain; reducing conditions) with the Western blot quantification in e . MAC-positive isolates in blue, and MAC-negative in red. Data show individual values as mean ± SD of at least three independent experiments. Representative Western blot membranes are shown. In a , two-way ANOVA with Dunnett’s post-test; in c , e , one-way ANOVA with Dunnett’s post-test; * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.

    Journal: Journal of Innate Immunity

    Article Title: Acinetobacter baumannii Clinical Isolates Resist Complement-Mediated Lysis by Inhibiting the Complement Cascade and Improperly Depositing MAC

    doi: 10.1159/000543664

    Figure Lengend Snippet: Deposition of C5b on bacteria. a C5b deposition on selected isolates by titration of NHS (0–10%) by flow cytometry. b C5b deposition on the bacterial surface (polyclonal antibody detecting highlighted α′ chain and β chain; reducing conditions) with the Western blot quantification in c . d C5b deposition on the bacterial surface (antibody detecting cleavage neoepitope in C5b α′ chain; reducing conditions) with the Western blot quantification in e . MAC-positive isolates in blue, and MAC-negative in red. Data show individual values as mean ± SD of at least three independent experiments. Representative Western blot membranes are shown. In a , two-way ANOVA with Dunnett’s post-test; in c , e , one-way ANOVA with Dunnett’s post-test; * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.

    Article Snippet: The following antibodies were used for the complement deposition analyses: polyclonal rabbit anti-human C3c (Dako, A0062); polyclonal goat anti-human C5 (Complement Technologies, A220); polyclonal rabbit anti-human C5b (specific for cleavage neoepitope) [ ]; monoclonal mouse anti-human C9 neoantigen (Hycult Biotech, HM2167); polyclonal goat anti-human C9 (Complement Technologies, A226); monoclonal mouse anti-human factor Bb (Quidel, A227); polyclonal goat anti-human factor P (Complement Technologies, A239).

    Techniques: Bacteria, Titration, Flow Cytometry, Western Blot