Structured Review

Korean Cell Line Bank human breast ductal carcinoma bt 474 cells
Human Breast Ductal Carcinoma Bt 474 Cells, supplied by Korean Cell Line Bank, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human breast ductal carcinoma bt 474 cells/product/Korean Cell Line Bank
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
human breast ductal carcinoma bt 474 cells - by Bioz Stars, 2024-07
86/100 stars

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Structured Review

Korean Cell Line Bank human breast carcinoma bt 474 cells
Phototoxicities were determined by a CCK-8 assay of MDA-MB-231, A549, <t>BT-474,</t> and NIH3T3 cells cultured in collagen gel-containing cell medium ( a ) with or ( b ) without hydroxyapatite particles. All cells were treated with free Ce6 (10 μg/mL) or each sample (equivalent Ce6 10 μg/mL) for 4 h and illuminated for 10 min at a light intensity of 5.2 mW/cm 2 using a 670 nm laser source (n = 7, as multiple experiments, ** p < 0.01 compared to free Ce6). A detailed description of each sample was given in the legend.
Human Breast Carcinoma Bt 474 Cells, supplied by Korean Cell Line Bank, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human breast carcinoma bt 474 cells/product/Korean Cell Line Bank
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
human breast carcinoma bt 474 cells - by Bioz Stars, 2024-07
86/100 stars

Images

1) Product Images from "Alendronate/cRGD-Decorated Ultrafine Hyaluronate Dot Targeting Bone Metastasis"

Article Title: Alendronate/cRGD-Decorated Ultrafine Hyaluronate Dot Targeting Bone Metastasis

Journal: Biomedicines

doi: 10.3390/biomedicines8110492

Phototoxicities were determined by a CCK-8 assay of MDA-MB-231, A549, BT-474, and NIH3T3 cells cultured in collagen gel-containing cell medium ( a ) with or ( b ) without hydroxyapatite particles. All cells were treated with free Ce6 (10 μg/mL) or each sample (equivalent Ce6 10 μg/mL) for 4 h and illuminated for 10 min at a light intensity of 5.2 mW/cm 2 using a 670 nm laser source (n = 7, as multiple experiments, ** p < 0.01 compared to free Ce6). A detailed description of each sample was given in the legend.
Figure Legend Snippet: Phototoxicities were determined by a CCK-8 assay of MDA-MB-231, A549, BT-474, and NIH3T3 cells cultured in collagen gel-containing cell medium ( a ) with or ( b ) without hydroxyapatite particles. All cells were treated with free Ce6 (10 μg/mL) or each sample (equivalent Ce6 10 μg/mL) for 4 h and illuminated for 10 min at a light intensity of 5.2 mW/cm 2 using a 670 nm laser source (n = 7, as multiple experiments, ** p < 0.01 compared to free Ce6). A detailed description of each sample was given in the legend.

Techniques Used: CCK-8 Assay, Cell Culture


Structured Review

Korean Cell Line Bank human breast carcinoma bt 474 cells
Flow cytometry profiles of ( a ) <t>BT-474,</t> ( b ) SK-N-MC and ( c ) Huh7 cells treated with free DOX (5 μg/mL) or EVs (equivalent to DOX of 5 μg/mL) for 4 h incubation at 37 °C.
Human Breast Carcinoma Bt 474 Cells, supplied by Korean Cell Line Bank, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human breast carcinoma bt 474 cells/product/Korean Cell Line Bank
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
human breast carcinoma bt 474 cells - by Bioz Stars, 2024-07
86/100 stars

Images

1) Product Images from "Tumor-Homing pH-Sensitive Extracellular Vesicles for Targeting Heterogeneous Tumors"

Article Title: Tumor-Homing pH-Sensitive Extracellular Vesicles for Targeting Heterogeneous Tumors

Journal: Pharmaceutics

doi: 10.3390/pharmaceutics12040372

Flow cytometry profiles of ( a ) BT-474, ( b ) SK-N-MC and ( c ) Huh7 cells treated with free DOX (5 μg/mL) or EVs (equivalent to DOX of 5 μg/mL) for 4 h incubation at 37 °C.
Figure Legend Snippet: Flow cytometry profiles of ( a ) BT-474, ( b ) SK-N-MC and ( c ) Huh7 cells treated with free DOX (5 μg/mL) or EVs (equivalent to DOX of 5 μg/mL) for 4 h incubation at 37 °C.

Techniques Used: Flow Cytometry, Incubation

Hyperspectral images of ( a ) BT-474 and ( b ) SK-N-MC cells treated with EVs (equivalent to DOX of 5 μg/mL) for 4 h incubation at 37 °C.
Figure Legend Snippet: Hyperspectral images of ( a ) BT-474 and ( b ) SK-N-MC cells treated with EVs (equivalent to DOX of 5 μg/mL) for 4 h incubation at 37 °C.

Techniques Used: Incubation

Confocal images of ( a ) BT-474 and ( b ) SK-N-MC cells treated with EVs (equivalent to DOX of 5 μg/mL) for 4 h incubation at 37 °C. The cells were stained using 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) and Wheat Germ Agglutinin Alexa Fluor ® 488 Conjugate (WGA-Alexa Fluor ® 488).
Figure Legend Snippet: Confocal images of ( a ) BT-474 and ( b ) SK-N-MC cells treated with EVs (equivalent to DOX of 5 μg/mL) for 4 h incubation at 37 °C. The cells were stained using 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) and Wheat Germ Agglutinin Alexa Fluor ® 488 Conjugate (WGA-Alexa Fluor ® 488).

Techniques Used: Incubation, Staining

( a ) Schematic illustration of in vitro experiments using Ce6 dye-incorporated HDEA@BTEVs, FITC dye-incorporated HDEA@SKEVs, and EV blends [Ce6 dye-incorporated HDEA@BTEVs/FITC dye-incorporated HDEA@SKEVs (50:50 wt.%)]. ( b ) Confocal images of BT-474 cells (left) and SK-N-MC cells (right) treated with Ce6 dye-incorporated HDEA@BTEVs, FITC dye-incorporated HDEA@SKEVs, or EV blends [Ce6 dye-incorporated HDEA@BTEVs/FITC dye-incorporated HDEA@SKEVs (50/50 wt.%)] (equivalent to EVs of 30 μg/mL) for 4 h incubation at 37 °C. ( c ) Cell viability determined by CCK-8 assay of tumor cells treated with EVs or EV blends [HDEA@BTEVs/HDEA@SKEVs (50/50 wt.%)] (equivalent to DOX of 5 μg/mL) for 4 h incubation at 37 °C ( n = 7, as multiple experiments, ** p < 0.01 compared with each EV sample).
Figure Legend Snippet: ( a ) Schematic illustration of in vitro experiments using Ce6 dye-incorporated HDEA@BTEVs, FITC dye-incorporated HDEA@SKEVs, and EV blends [Ce6 dye-incorporated HDEA@BTEVs/FITC dye-incorporated HDEA@SKEVs (50:50 wt.%)]. ( b ) Confocal images of BT-474 cells (left) and SK-N-MC cells (right) treated with Ce6 dye-incorporated HDEA@BTEVs, FITC dye-incorporated HDEA@SKEVs, or EV blends [Ce6 dye-incorporated HDEA@BTEVs/FITC dye-incorporated HDEA@SKEVs (50/50 wt.%)] (equivalent to EVs of 30 μg/mL) for 4 h incubation at 37 °C. ( c ) Cell viability determined by CCK-8 assay of tumor cells treated with EVs or EV blends [HDEA@BTEVs/HDEA@SKEVs (50/50 wt.%)] (equivalent to DOX of 5 μg/mL) for 4 h incubation at 37 °C ( n = 7, as multiple experiments, ** p < 0.01 compared with each EV sample).

Techniques Used: In Vitro, Incubation, CCK-8 Assay

Cell viability determined by Cell Counting Kit-8 (CCK-8) assay of ( a ) BT-474, ( b ) SK-N-MC and ( c ) Huh7 cells treated with free DOX (5 μg/mL) or EVs (equivalent to DOX of 5 μg/mL) for 4 h incubation at 37 °C ( n = 7, as multiple experiments), (** p < 0.01 compared with the free DOX). Cell viability determined by CCK-8 assay of ( d ) BT-474, ( e ) SK-N-MC, and ( f ) Huh7 cells treated with blank EVs (without DOX) for 24 h incubation at 37 °C ( n = 7, as multiple experiments).
Figure Legend Snippet: Cell viability determined by Cell Counting Kit-8 (CCK-8) assay of ( a ) BT-474, ( b ) SK-N-MC and ( c ) Huh7 cells treated with free DOX (5 μg/mL) or EVs (equivalent to DOX of 5 μg/mL) for 4 h incubation at 37 °C ( n = 7, as multiple experiments), (** p < 0.01 compared with the free DOX). Cell viability determined by CCK-8 assay of ( d ) BT-474, ( e ) SK-N-MC, and ( f ) Huh7 cells treated with blank EVs (without DOX) for 24 h incubation at 37 °C ( n = 7, as multiple experiments).

Techniques Used: Cell Counting, CCK-8 Assay, Incubation


Structured Review

Korean Cell Line Bank human breast carcinoma bt 474 cells
Flow cytometry profiles of ( a ) <t>BT-474,</t> ( b ) SK-N-MC and ( c ) Huh7 cells treated with free DOX (5 μg/mL) or EVs (equivalent to DOX of 5 μg/mL) for 4 h incubation at 37 °C.
Human Breast Carcinoma Bt 474 Cells, supplied by Korean Cell Line Bank, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human breast carcinoma bt 474 cells/product/Korean Cell Line Bank
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
human breast carcinoma bt 474 cells - by Bioz Stars, 2024-07
86/100 stars

Images

1) Product Images from "Tumor-Homing pH-Sensitive Extracellular Vesicles for Targeting Heterogeneous Tumors"

Article Title: Tumor-Homing pH-Sensitive Extracellular Vesicles for Targeting Heterogeneous Tumors

Journal: Pharmaceutics

doi: 10.3390/pharmaceutics12040372

Flow cytometry profiles of ( a ) BT-474, ( b ) SK-N-MC and ( c ) Huh7 cells treated with free DOX (5 μg/mL) or EVs (equivalent to DOX of 5 μg/mL) for 4 h incubation at 37 °C.
Figure Legend Snippet: Flow cytometry profiles of ( a ) BT-474, ( b ) SK-N-MC and ( c ) Huh7 cells treated with free DOX (5 μg/mL) or EVs (equivalent to DOX of 5 μg/mL) for 4 h incubation at 37 °C.

Techniques Used: Flow Cytometry, Incubation

Hyperspectral images of ( a ) BT-474 and ( b ) SK-N-MC cells treated with EVs (equivalent to DOX of 5 μg/mL) for 4 h incubation at 37 °C.
Figure Legend Snippet: Hyperspectral images of ( a ) BT-474 and ( b ) SK-N-MC cells treated with EVs (equivalent to DOX of 5 μg/mL) for 4 h incubation at 37 °C.

Techniques Used: Incubation

Confocal images of ( a ) BT-474 and ( b ) SK-N-MC cells treated with EVs (equivalent to DOX of 5 μg/mL) for 4 h incubation at 37 °C. The cells were stained using 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) and Wheat Germ Agglutinin Alexa Fluor ® 488 Conjugate (WGA-Alexa Fluor ® 488).
Figure Legend Snippet: Confocal images of ( a ) BT-474 and ( b ) SK-N-MC cells treated with EVs (equivalent to DOX of 5 μg/mL) for 4 h incubation at 37 °C. The cells were stained using 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) and Wheat Germ Agglutinin Alexa Fluor ® 488 Conjugate (WGA-Alexa Fluor ® 488).

Techniques Used: Incubation, Staining

( a ) Schematic illustration of in vitro experiments using Ce6 dye-incorporated HDEA@BTEVs, FITC dye-incorporated HDEA@SKEVs, and EV blends [Ce6 dye-incorporated HDEA@BTEVs/FITC dye-incorporated HDEA@SKEVs (50:50 wt.%)]. ( b ) Confocal images of BT-474 cells (left) and SK-N-MC cells (right) treated with Ce6 dye-incorporated HDEA@BTEVs, FITC dye-incorporated HDEA@SKEVs, or EV blends [Ce6 dye-incorporated HDEA@BTEVs/FITC dye-incorporated HDEA@SKEVs (50/50 wt.%)] (equivalent to EVs of 30 μg/mL) for 4 h incubation at 37 °C. ( c ) Cell viability determined by CCK-8 assay of tumor cells treated with EVs or EV blends [HDEA@BTEVs/HDEA@SKEVs (50/50 wt.%)] (equivalent to DOX of 5 μg/mL) for 4 h incubation at 37 °C ( n = 7, as multiple experiments, ** p < 0.01 compared with each EV sample).
Figure Legend Snippet: ( a ) Schematic illustration of in vitro experiments using Ce6 dye-incorporated HDEA@BTEVs, FITC dye-incorporated HDEA@SKEVs, and EV blends [Ce6 dye-incorporated HDEA@BTEVs/FITC dye-incorporated HDEA@SKEVs (50:50 wt.%)]. ( b ) Confocal images of BT-474 cells (left) and SK-N-MC cells (right) treated with Ce6 dye-incorporated HDEA@BTEVs, FITC dye-incorporated HDEA@SKEVs, or EV blends [Ce6 dye-incorporated HDEA@BTEVs/FITC dye-incorporated HDEA@SKEVs (50/50 wt.%)] (equivalent to EVs of 30 μg/mL) for 4 h incubation at 37 °C. ( c ) Cell viability determined by CCK-8 assay of tumor cells treated with EVs or EV blends [HDEA@BTEVs/HDEA@SKEVs (50/50 wt.%)] (equivalent to DOX of 5 μg/mL) for 4 h incubation at 37 °C ( n = 7, as multiple experiments, ** p < 0.01 compared with each EV sample).

Techniques Used: In Vitro, Incubation, CCK-8 Assay

Cell viability determined by Cell Counting Kit-8 (CCK-8) assay of ( a ) BT-474, ( b ) SK-N-MC and ( c ) Huh7 cells treated with free DOX (5 μg/mL) or EVs (equivalent to DOX of 5 μg/mL) for 4 h incubation at 37 °C ( n = 7, as multiple experiments), (** p < 0.01 compared with the free DOX). Cell viability determined by CCK-8 assay of ( d ) BT-474, ( e ) SK-N-MC, and ( f ) Huh7 cells treated with blank EVs (without DOX) for 24 h incubation at 37 °C ( n = 7, as multiple experiments).
Figure Legend Snippet: Cell viability determined by Cell Counting Kit-8 (CCK-8) assay of ( a ) BT-474, ( b ) SK-N-MC and ( c ) Huh7 cells treated with free DOX (5 μg/mL) or EVs (equivalent to DOX of 5 μg/mL) for 4 h incubation at 37 °C ( n = 7, as multiple experiments), (** p < 0.01 compared with the free DOX). Cell viability determined by CCK-8 assay of ( d ) BT-474, ( e ) SK-N-MC, and ( f ) Huh7 cells treated with blank EVs (without DOX) for 24 h incubation at 37 °C ( n = 7, as multiple experiments).

Techniques Used: Cell Counting, CCK-8 Assay, Incubation


Structured Review

Korean Cell Line Bank bt 474 human breast carcinoma cell lines
Bt 474 Human Breast Carcinoma Cell Lines, supplied by Korean Cell Line Bank, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bt 474 human breast carcinoma cell lines/product/Korean Cell Line Bank
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
bt 474 human breast carcinoma cell lines - by Bioz Stars, 2024-07
86/100 stars

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    Korean Cell Line Bank human breast ductal carcinoma bt 474 cells
    Human Breast Ductal Carcinoma Bt 474 Cells, supplied by Korean Cell Line Bank, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human breast ductal carcinoma bt 474 cells/product/Korean Cell Line Bank
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human breast ductal carcinoma bt 474 cells - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    Korean Cell Line Bank human breast carcinoma bt 474 cells
    Phototoxicities were determined by a CCK-8 assay of MDA-MB-231, A549, <t>BT-474,</t> and NIH3T3 cells cultured in collagen gel-containing cell medium ( a ) with or ( b ) without hydroxyapatite particles. All cells were treated with free Ce6 (10 μg/mL) or each sample (equivalent Ce6 10 μg/mL) for 4 h and illuminated for 10 min at a light intensity of 5.2 mW/cm 2 using a 670 nm laser source (n = 7, as multiple experiments, ** p < 0.01 compared to free Ce6). A detailed description of each sample was given in the legend.
    Human Breast Carcinoma Bt 474 Cells, supplied by Korean Cell Line Bank, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human breast carcinoma bt 474 cells/product/Korean Cell Line Bank
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human breast carcinoma bt 474 cells - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    Korean Cell Line Bank bt 474 human breast carcinoma cell lines
    Phototoxicities were determined by a CCK-8 assay of MDA-MB-231, A549, <t>BT-474,</t> and NIH3T3 cells cultured in collagen gel-containing cell medium ( a ) with or ( b ) without hydroxyapatite particles. All cells were treated with free Ce6 (10 μg/mL) or each sample (equivalent Ce6 10 μg/mL) for 4 h and illuminated for 10 min at a light intensity of 5.2 mW/cm 2 using a 670 nm laser source (n = 7, as multiple experiments, ** p < 0.01 compared to free Ce6). A detailed description of each sample was given in the legend.
    Bt 474 Human Breast Carcinoma Cell Lines, supplied by Korean Cell Line Bank, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bt 474 human breast carcinoma cell lines/product/Korean Cell Line Bank
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bt 474 human breast carcinoma cell lines - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

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    Phototoxicities were determined by a CCK-8 assay of MDA-MB-231, A549, BT-474, and NIH3T3 cells cultured in collagen gel-containing cell medium ( a ) with or ( b ) without hydroxyapatite particles. All cells were treated with free Ce6 (10 μg/mL) or each sample (equivalent Ce6 10 μg/mL) for 4 h and illuminated for 10 min at a light intensity of 5.2 mW/cm 2 using a 670 nm laser source (n = 7, as multiple experiments, ** p < 0.01 compared to free Ce6). A detailed description of each sample was given in the legend.

    Journal: Biomedicines

    Article Title: Alendronate/cRGD-Decorated Ultrafine Hyaluronate Dot Targeting Bone Metastasis

    doi: 10.3390/biomedicines8110492

    Figure Lengend Snippet: Phototoxicities were determined by a CCK-8 assay of MDA-MB-231, A549, BT-474, and NIH3T3 cells cultured in collagen gel-containing cell medium ( a ) with or ( b ) without hydroxyapatite particles. All cells were treated with free Ce6 (10 μg/mL) or each sample (equivalent Ce6 10 μg/mL) for 4 h and illuminated for 10 min at a light intensity of 5.2 mW/cm 2 using a 670 nm laser source (n = 7, as multiple experiments, ** p < 0.01 compared to free Ce6). A detailed description of each sample was given in the legend.

    Article Snippet: Human breast carcinoma MDA-MB-231 cells, human lung carcinoma A549 cells, human breast carcinoma BT-474 cells, and mouse embryo fibroblast NIH3T3 cells were purchased from the Korean Cell Line Bank.

    Techniques: CCK-8 Assay, Cell Culture