human breast cancer cell line mcf 7  (ATCC)


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    ATCC human breast cancer cell line mcf 7
    Human Breast Cancer Cell Line Mcf 7, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human breast cancer cell lines  (ATCC)


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    ATCC human breast cancer cell lines
    Human Breast Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human breast cancer cell line mcf 7  (ATCC)


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    ATCC human breast cancer cell line mcf 7
    MTT and ATP Assays in 2D and 3D Cell Cultures. ( a ) Cytotoxicity of PhO <t>to</t> <t>MCF-7</t> <t>cells</t> after 24-h exposure determined by the MTT assay. ( b ) Comparison of MTT assays with previously published results . Cytotoxic effects of PhO using an ATP assay in <t>2D</t> <t>MCF-7</t> <t>cell</t> culture in a concentration- ( c ) and time- ( e ) dependent manner. Cytotoxic effects of PhO using an ATP assay in 3D MCF-7 spheroids in a concentration- ( d ) and time- ( f ) dependent manner. Statistical significance for ( e , f ): was determined using the two-way ANOVA followed by the Dunnett’s post hoc test. * p < 0.033, ** p < 0.002, *** p < 0.001. ( g ) Comparison of cytotoxicity between the (ATP) ATP assay (this study) and previously published Crystal Violet (CV) assays. B14 ( Cricetulus griseus ), BRL ( Rattus norvegicus ), NRK ( Rattus norvegicus ) mammalian cell lines. Red asterisk indicates previous work by . DDM—dendrimer.
    Human Breast Cancer Cell Line Mcf 7, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Biodistribution and toxicity assessment of methoxyphenyl phosphonium carbosilane dendrimers in 2D and 3D cell cultures of human cancer cells and zebrafish embryos"

    Article Title: Biodistribution and toxicity assessment of methoxyphenyl phosphonium carbosilane dendrimers in 2D and 3D cell cultures of human cancer cells and zebrafish embryos

    Journal: Scientific Reports

    doi: 10.1038/s41598-023-42850-3

    MTT and ATP Assays in 2D and 3D Cell Cultures. ( a ) Cytotoxicity of PhO to MCF-7 cells after 24-h exposure determined by the MTT assay. ( b ) Comparison of MTT assays with previously published results . Cytotoxic effects of PhO using an ATP assay in 2D MCF-7 cell culture in a concentration- ( c ) and time- ( e ) dependent manner. Cytotoxic effects of PhO using an ATP assay in 3D MCF-7 spheroids in a concentration- ( d ) and time- ( f ) dependent manner. Statistical significance for ( e , f ): was determined using the two-way ANOVA followed by the Dunnett’s post hoc test. * p < 0.033, ** p < 0.002, *** p < 0.001. ( g ) Comparison of cytotoxicity between the (ATP) ATP assay (this study) and previously published Crystal Violet (CV) assays. B14 ( Cricetulus griseus ), BRL ( Rattus norvegicus ), NRK ( Rattus norvegicus ) mammalian cell lines. Red asterisk indicates previous work by . DDM—dendrimer.
    Figure Legend Snippet: MTT and ATP Assays in 2D and 3D Cell Cultures. ( a ) Cytotoxicity of PhO to MCF-7 cells after 24-h exposure determined by the MTT assay. ( b ) Comparison of MTT assays with previously published results . Cytotoxic effects of PhO using an ATP assay in 2D MCF-7 cell culture in a concentration- ( c ) and time- ( e ) dependent manner. Cytotoxic effects of PhO using an ATP assay in 3D MCF-7 spheroids in a concentration- ( d ) and time- ( f ) dependent manner. Statistical significance for ( e , f ): was determined using the two-way ANOVA followed by the Dunnett’s post hoc test. * p < 0.033, ** p < 0.002, *** p < 0.001. ( g ) Comparison of cytotoxicity between the (ATP) ATP assay (this study) and previously published Crystal Violet (CV) assays. B14 ( Cricetulus griseus ), BRL ( Rattus norvegicus ), NRK ( Rattus norvegicus ) mammalian cell lines. Red asterisk indicates previous work by . DDM—dendrimer.

    Techniques Used: MTT Assay, Comparison, ATP Assay, Cell Culture, Concentration Assay

    3D In vitro Assays. ( a ) Dendrimer cytotoxicity as represented by MCF-7 spheroid growth curves. The levels of significance: *** p < 0.001, between dendrimer concentrations and the control. Statistical significance was determined using the two-way ANOVA followed by the Dunnett’s post hoc test. ( b ) Changes in spheroid morphology after incubation with PhO. (-) Control spheroids incubated without PhO. Scale bars = 200 μm.
    Figure Legend Snippet: 3D In vitro Assays. ( a ) Dendrimer cytotoxicity as represented by MCF-7 spheroid growth curves. The levels of significance: *** p < 0.001, between dendrimer concentrations and the control. Statistical significance was determined using the two-way ANOVA followed by the Dunnett’s post hoc test. ( b ) Changes in spheroid morphology after incubation with PhO. (-) Control spheroids incubated without PhO. Scale bars = 200 μm.

    Techniques Used: In Vitro, Incubation

    Biodistribution of the PhO-Cy5 Conjugate in Spheroids. ( a ) A maximum intensity projection (MIP) of a confocal stack of an MCF-7 spheroid cultivated for 72 h in the presence of 1 µmol/L PhO-Cy5. A single slice is shown with an inset highlighting the mitochondrial localization of PhO-Cy5. The images of individual channels of the shown slice were despeckled using Fiji and the insets were further adjusted using 0.75 σ Gaussian blur. Hoechst (blue), Mitotracker Red (green), and PhO-Cy5 (magenta). Black scale bar = 100 μm, white scale bar = 10 μm. ( b ) Another MCF-7 spheroid stained identically as in ( a ), imaged with an oil 63x/1.40 N.A. objective to improve the resolution for visualising mitochondrial localization. Scale bar = 25 μm. ( b ) MIP of a control MCF-7 spheroid cultivated without PhO-Cy5, stained and imaged identically as in ( a) . BF—Bright Field.
    Figure Legend Snippet: Biodistribution of the PhO-Cy5 Conjugate in Spheroids. ( a ) A maximum intensity projection (MIP) of a confocal stack of an MCF-7 spheroid cultivated for 72 h in the presence of 1 µmol/L PhO-Cy5. A single slice is shown with an inset highlighting the mitochondrial localization of PhO-Cy5. The images of individual channels of the shown slice were despeckled using Fiji and the insets were further adjusted using 0.75 σ Gaussian blur. Hoechst (blue), Mitotracker Red (green), and PhO-Cy5 (magenta). Black scale bar = 100 μm, white scale bar = 10 μm. ( b ) Another MCF-7 spheroid stained identically as in ( a ), imaged with an oil 63x/1.40 N.A. objective to improve the resolution for visualising mitochondrial localization. Scale bar = 25 μm. ( b ) MIP of a control MCF-7 spheroid cultivated without PhO-Cy5, stained and imaged identically as in ( a) . BF—Bright Field.

    Techniques Used: Staining

    human breast cancer cell line mcf7  (ATCC)


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    ATCC human breast cancer cell line mcf7
    Selective recognition of Lewis Y by germline antibodies. (A) Bar graph showing signals in relative fluorescence units (RFUs) for antibody 8F11 on our 738-component array. Data are shown at a concentration approximately 10-fold higher than the apparent KD value for LeY-tetra to highlight any potential cross-reactivity. Positive and negative controls have been omitted for clarity. Structures are depicted for glycans with signals over 2000 RFU. LeY tetra and LeY-08 have different linkers—see Figure 1. (B) Direct comparison of germline anti-Lewis Y antibody binding to a subset of Lewis Y related glycans. (C) Binding of 8F11 to the Lewis Y positive <t>MCF7</t> cell line (left) and to the Lewis Y negative SK-MEL-28 cell line (right) as compared to an IgM isotype control. Bar graphs for 12D6 and 8G6 can be found in Figure S3. Additional flow cytometry data can be found in Figures S4 and S5.
    Human Breast Cancer Cell Line Mcf7, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Selective Recognition of Carbohydrate Antigens by Germline Antibodies Isolated from AID Knockout Mice"

    Article Title: Selective Recognition of Carbohydrate Antigens by Germline Antibodies Isolated from AID Knockout Mice

    Journal: Journal of the American Chemical Society

    doi: 10.1021/jacs.1c12745

    Selective recognition of Lewis Y by germline antibodies. (A) Bar graph showing signals in relative fluorescence units (RFUs) for antibody 8F11 on our 738-component array. Data are shown at a concentration approximately 10-fold higher than the apparent KD value for LeY-tetra to highlight any potential cross-reactivity. Positive and negative controls have been omitted for clarity. Structures are depicted for glycans with signals over 2000 RFU. LeY tetra and LeY-08 have different linkers—see Figure 1. (B) Direct comparison of germline anti-Lewis Y antibody binding to a subset of Lewis Y related glycans. (C) Binding of 8F11 to the Lewis Y positive MCF7 cell line (left) and to the Lewis Y negative SK-MEL-28 cell line (right) as compared to an IgM isotype control. Bar graphs for 12D6 and 8G6 can be found in Figure S3. Additional flow cytometry data can be found in Figures S4 and S5.
    Figure Legend Snippet: Selective recognition of Lewis Y by germline antibodies. (A) Bar graph showing signals in relative fluorescence units (RFUs) for antibody 8F11 on our 738-component array. Data are shown at a concentration approximately 10-fold higher than the apparent KD value for LeY-tetra to highlight any potential cross-reactivity. Positive and negative controls have been omitted for clarity. Structures are depicted for glycans with signals over 2000 RFU. LeY tetra and LeY-08 have different linkers—see Figure 1. (B) Direct comparison of germline anti-Lewis Y antibody binding to a subset of Lewis Y related glycans. (C) Binding of 8F11 to the Lewis Y positive MCF7 cell line (left) and to the Lewis Y negative SK-MEL-28 cell line (right) as compared to an IgM isotype control. Bar graphs for 12D6 and 8G6 can be found in Figure S3. Additional flow cytometry data can be found in Figures S4 and S5.

    Techniques Used: Fluorescence, Concentration Assay, Comparison, Binding Assay, Flow Cytometry

    Binding properties of representative germline antibodies to Lewis X, sialyl Lewis C, and the Tn antigen. (A) Signals in relative fluorescence units (RFUs) for antibodies on our 738-component array. Data for 11A11, 9A9, and 8C11 are at a concentration approximately 20-fold higher than KD,App for the primary antigen; 2D3.C11 is at 8 μg/mL. Positive and negative controls have been excluded. (B) Binding data for anti-Lewis X antibodies to Lewis X positive MCF7 cell line and Lewis X negative SK-MEL-28 as compared to positive control anti-Lewis X antibody MC-480. (C) Binding data for anti-Tn antibodies to Tn positive MCF7 cell line and Tn negative HEK-293 cell line as compared to positive control anti-Tn antibody SBH-Tn. Error bars (B,C) represent the SD of three experiments. Additional bar graphs can be found in Figure S8. Representative histograms and additional flow cytometry data can be found in Figure S9.
    Figure Legend Snippet: Binding properties of representative germline antibodies to Lewis X, sialyl Lewis C, and the Tn antigen. (A) Signals in relative fluorescence units (RFUs) for antibodies on our 738-component array. Data for 11A11, 9A9, and 8C11 are at a concentration approximately 20-fold higher than KD,App for the primary antigen; 2D3.C11 is at 8 μg/mL. Positive and negative controls have been excluded. (B) Binding data for anti-Lewis X antibodies to Lewis X positive MCF7 cell line and Lewis X negative SK-MEL-28 as compared to positive control anti-Lewis X antibody MC-480. (C) Binding data for anti-Tn antibodies to Tn positive MCF7 cell line and Tn negative HEK-293 cell line as compared to positive control anti-Tn antibody SBH-Tn. Error bars (B,C) represent the SD of three experiments. Additional bar graphs can be found in Figure S8. Representative histograms and additional flow cytometry data can be found in Figure S9.

    Techniques Used: Binding Assay, Fluorescence, Concentration Assay, Positive Control, Flow Cytometry

    human breast cancer cell line mcf7  (ATCC)


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    ATCC human breast cancer cell line mcf7
    Selective recognition of Lewis Y by germline antibodies. (A) Bar graph showing signals in relative fluorescence units (RFUs) for antibody 8F11 on our 738-component array. Data are shown at a concentration approximately 10-fold higher than the apparent KD value for LeY-tetra to highlight any potential cross-reactivity. Positive and negative controls have been omitted for clarity. Structures are depicted for glycans with signals over 2000 RFU. LeY tetra and LeY-08 have different linkers—see Figure 1. (B) Direct comparison of germline anti-Lewis Y antibody binding to a subset of Lewis Y related glycans. (C) Binding of 8F11 to the Lewis Y positive <t>MCF7</t> cell line (left) and to the Lewis Y negative SK-MEL-28 cell line (right) as compared to an IgM isotype control. Bar graphs for 12D6 and 8G6 can be found in Figure S3. Additional flow cytometry data can be found in Figures S4 and S5.
    Human Breast Cancer Cell Line Mcf7, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human breast cancer cell line mcf7/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human breast cancer cell line mcf7 - by Bioz Stars, 2023-09
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    Images

    1) Product Images from "Selective Recognition of Carbohydrate Antigens by Germline Antibodies Isolated from AID Knockout Mice"

    Article Title: Selective Recognition of Carbohydrate Antigens by Germline Antibodies Isolated from AID Knockout Mice

    Journal: Journal of the American Chemical Society

    doi: 10.1021/jacs.1c12745

    Selective recognition of Lewis Y by germline antibodies. (A) Bar graph showing signals in relative fluorescence units (RFUs) for antibody 8F11 on our 738-component array. Data are shown at a concentration approximately 10-fold higher than the apparent KD value for LeY-tetra to highlight any potential cross-reactivity. Positive and negative controls have been omitted for clarity. Structures are depicted for glycans with signals over 2000 RFU. LeY tetra and LeY-08 have different linkers—see Figure 1. (B) Direct comparison of germline anti-Lewis Y antibody binding to a subset of Lewis Y related glycans. (C) Binding of 8F11 to the Lewis Y positive MCF7 cell line (left) and to the Lewis Y negative SK-MEL-28 cell line (right) as compared to an IgM isotype control. Bar graphs for 12D6 and 8G6 can be found in Figure S3. Additional flow cytometry data can be found in Figures S4 and S5.
    Figure Legend Snippet: Selective recognition of Lewis Y by germline antibodies. (A) Bar graph showing signals in relative fluorescence units (RFUs) for antibody 8F11 on our 738-component array. Data are shown at a concentration approximately 10-fold higher than the apparent KD value for LeY-tetra to highlight any potential cross-reactivity. Positive and negative controls have been omitted for clarity. Structures are depicted for glycans with signals over 2000 RFU. LeY tetra and LeY-08 have different linkers—see Figure 1. (B) Direct comparison of germline anti-Lewis Y antibody binding to a subset of Lewis Y related glycans. (C) Binding of 8F11 to the Lewis Y positive MCF7 cell line (left) and to the Lewis Y negative SK-MEL-28 cell line (right) as compared to an IgM isotype control. Bar graphs for 12D6 and 8G6 can be found in Figure S3. Additional flow cytometry data can be found in Figures S4 and S5.

    Techniques Used: Fluorescence, Concentration Assay, Comparison, Binding Assay, Flow Cytometry

    Binding properties of representative germline antibodies to Lewis X, sialyl Lewis C, and the Tn antigen. (A) Signals in relative fluorescence units (RFUs) for antibodies on our 738-component array. Data for 11A11, 9A9, and 8C11 are at a concentration approximately 20-fold higher than KD,App for the primary antigen; 2D3.C11 is at 8 μg/mL. Positive and negative controls have been excluded. (B) Binding data for anti-Lewis X antibodies to Lewis X positive MCF7 cell line and Lewis X negative SK-MEL-28 as compared to positive control anti-Lewis X antibody MC-480. (C) Binding data for anti-Tn antibodies to Tn positive MCF7 cell line and Tn negative HEK-293 cell line as compared to positive control anti-Tn antibody SBH-Tn. Error bars (B,C) represent the SD of three experiments. Additional bar graphs can be found in Figure S8. Representative histograms and additional flow cytometry data can be found in Figure S9.
    Figure Legend Snippet: Binding properties of representative germline antibodies to Lewis X, sialyl Lewis C, and the Tn antigen. (A) Signals in relative fluorescence units (RFUs) for antibodies on our 738-component array. Data for 11A11, 9A9, and 8C11 are at a concentration approximately 20-fold higher than KD,App for the primary antigen; 2D3.C11 is at 8 μg/mL. Positive and negative controls have been excluded. (B) Binding data for anti-Lewis X antibodies to Lewis X positive MCF7 cell line and Lewis X negative SK-MEL-28 as compared to positive control anti-Lewis X antibody MC-480. (C) Binding data for anti-Tn antibodies to Tn positive MCF7 cell line and Tn negative HEK-293 cell line as compared to positive control anti-Tn antibody SBH-Tn. Error bars (B,C) represent the SD of three experiments. Additional bar graphs can be found in Figure S8. Representative histograms and additional flow cytometry data can be found in Figure S9.

    Techniques Used: Binding Assay, Fluorescence, Concentration Assay, Positive Control, Flow Cytometry

    human breast cancer cell lines  (ATCC)


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    ATCC human breast cancer cell lines
    Human Breast Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human breast cancer cell lines  (ATCC)


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    ATCC human breast cancer cell lines
    Human Breast Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human breast cancer cell lines t 47d  (ATCC)


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    ATCC human breast cancer cell lines t 47d
    Human Breast Cancer Cell Lines T 47d, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human breast cancer cell lines  (ATCC)


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    ATCC human breast cancer cell lines
    Human Breast Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human breast cancer cell line  (ATCC)


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    ATCC human breast cancer cell line
    Human Breast Cancer Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC human breast cancer cell line mcf 7
    Human Breast Cancer Cell Line Mcf 7, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human breast cancer cell line mcf 7/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human breast cancer cell line mcf 7 - by Bioz Stars, 2023-09
    86/100 stars
    		  Buy from Supplier
    human breast cancer cell lines  (ATCC)
    86
    ATCC human breast cancer cell lines
    Human Breast Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human breast cancer cell lines/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human breast cancer cell lines - by Bioz Stars, 2023-09
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    human breast cancer cell line mcf7  (ATCC)
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    ATCC human breast cancer cell line mcf7
    Selective recognition of Lewis Y by germline antibodies. (A) Bar graph showing signals in relative fluorescence units (RFUs) for antibody 8F11 on our 738-component array. Data are shown at a concentration approximately 10-fold higher than the apparent KD value for LeY-tetra to highlight any potential cross-reactivity. Positive and negative controls have been omitted for clarity. Structures are depicted for glycans with signals over 2000 RFU. LeY tetra and LeY-08 have different linkers—see Figure 1. (B) Direct comparison of germline anti-Lewis Y antibody binding to a subset of Lewis Y related glycans. (C) Binding of 8F11 to the Lewis Y positive <t>MCF7</t> cell line (left) and to the Lewis Y negative SK-MEL-28 cell line (right) as compared to an IgM isotype control. Bar graphs for 12D6 and 8G6 can be found in Figure S3. Additional flow cytometry data can be found in Figures S4 and S5.
    Human Breast Cancer Cell Line Mcf7, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human breast cancer cell line mcf7/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human breast cancer cell line mcf7 - by Bioz Stars, 2023-09
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    human breast cancer cell lines t 47d  (ATCC)
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    ATCC human breast cancer cell lines t 47d
    Selective recognition of Lewis Y by germline antibodies. (A) Bar graph showing signals in relative fluorescence units (RFUs) for antibody 8F11 on our 738-component array. Data are shown at a concentration approximately 10-fold higher than the apparent KD value for LeY-tetra to highlight any potential cross-reactivity. Positive and negative controls have been omitted for clarity. Structures are depicted for glycans with signals over 2000 RFU. LeY tetra and LeY-08 have different linkers—see Figure 1. (B) Direct comparison of germline anti-Lewis Y antibody binding to a subset of Lewis Y related glycans. (C) Binding of 8F11 to the Lewis Y positive <t>MCF7</t> cell line (left) and to the Lewis Y negative SK-MEL-28 cell line (right) as compared to an IgM isotype control. Bar graphs for 12D6 and 8G6 can be found in Figure S3. Additional flow cytometry data can be found in Figures S4 and S5.
    Human Breast Cancer Cell Lines T 47d, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human breast cancer cell lines t 47d/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human breast cancer cell lines t 47d - by Bioz Stars, 2023-09
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    human breast cancer cell line  (ATCC)
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    ATCC human breast cancer cell line
    Selective recognition of Lewis Y by germline antibodies. (A) Bar graph showing signals in relative fluorescence units (RFUs) for antibody 8F11 on our 738-component array. Data are shown at a concentration approximately 10-fold higher than the apparent KD value for LeY-tetra to highlight any potential cross-reactivity. Positive and negative controls have been omitted for clarity. Structures are depicted for glycans with signals over 2000 RFU. LeY tetra and LeY-08 have different linkers—see Figure 1. (B) Direct comparison of germline anti-Lewis Y antibody binding to a subset of Lewis Y related glycans. (C) Binding of 8F11 to the Lewis Y positive <t>MCF7</t> cell line (left) and to the Lewis Y negative SK-MEL-28 cell line (right) as compared to an IgM isotype control. Bar graphs for 12D6 and 8G6 can be found in Figure S3. Additional flow cytometry data can be found in Figures S4 and S5.
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    Selective recognition of Lewis Y by germline antibodies. (A) Bar graph showing signals in relative fluorescence units (RFUs) for antibody 8F11 on our 738-component array. Data are shown at a concentration approximately 10-fold higher than the apparent KD value for LeY-tetra to highlight any potential cross-reactivity. Positive and negative controls have been omitted for clarity. Structures are depicted for glycans with signals over 2000 RFU. LeY tetra and LeY-08 have different linkers—see Figure 1. (B) Direct comparison of germline anti-Lewis Y antibody binding to a subset of Lewis Y related glycans. (C) Binding of 8F11 to the Lewis Y positive MCF7 cell line (left) and to the Lewis Y negative SK-MEL-28 cell line (right) as compared to an IgM isotype control. Bar graphs for 12D6 and 8G6 can be found in Figure S3. Additional flow cytometry data can be found in Figures S4 and S5.

    Journal: Journal of the American Chemical Society

    Article Title: Selective Recognition of Carbohydrate Antigens by Germline Antibodies Isolated from AID Knockout Mice

    doi: 10.1021/jacs.1c12745

    Figure Lengend Snippet: Selective recognition of Lewis Y by germline antibodies. (A) Bar graph showing signals in relative fluorescence units (RFUs) for antibody 8F11 on our 738-component array. Data are shown at a concentration approximately 10-fold higher than the apparent KD value for LeY-tetra to highlight any potential cross-reactivity. Positive and negative controls have been omitted for clarity. Structures are depicted for glycans with signals over 2000 RFU. LeY tetra and LeY-08 have different linkers—see Figure 1. (B) Direct comparison of germline anti-Lewis Y antibody binding to a subset of Lewis Y related glycans. (C) Binding of 8F11 to the Lewis Y positive MCF7 cell line (left) and to the Lewis Y negative SK-MEL-28 cell line (right) as compared to an IgM isotype control. Bar graphs for 12D6 and 8G6 can be found in Figure S3. Additional flow cytometry data can be found in Figures S4 and S5.

    Article Snippet: The human breast cancer cell line MCF7 was generously provided by the NCI Developmental Therapeutics Program and was authenticated by ATCC’s cell line authentication service (ATCC 135-XV).

    Techniques: Fluorescence, Concentration Assay, Comparison, Binding Assay, Flow Cytometry

    Binding properties of representative germline antibodies to Lewis X, sialyl Lewis C, and the Tn antigen. (A) Signals in relative fluorescence units (RFUs) for antibodies on our 738-component array. Data for 11A11, 9A9, and 8C11 are at a concentration approximately 20-fold higher than KD,App for the primary antigen; 2D3.C11 is at 8 μg/mL. Positive and negative controls have been excluded. (B) Binding data for anti-Lewis X antibodies to Lewis X positive MCF7 cell line and Lewis X negative SK-MEL-28 as compared to positive control anti-Lewis X antibody MC-480. (C) Binding data for anti-Tn antibodies to Tn positive MCF7 cell line and Tn negative HEK-293 cell line as compared to positive control anti-Tn antibody SBH-Tn. Error bars (B,C) represent the SD of three experiments. Additional bar graphs can be found in Figure S8. Representative histograms and additional flow cytometry data can be found in Figure S9.

    Journal: Journal of the American Chemical Society

    Article Title: Selective Recognition of Carbohydrate Antigens by Germline Antibodies Isolated from AID Knockout Mice

    doi: 10.1021/jacs.1c12745

    Figure Lengend Snippet: Binding properties of representative germline antibodies to Lewis X, sialyl Lewis C, and the Tn antigen. (A) Signals in relative fluorescence units (RFUs) for antibodies on our 738-component array. Data for 11A11, 9A9, and 8C11 are at a concentration approximately 20-fold higher than KD,App for the primary antigen; 2D3.C11 is at 8 μg/mL. Positive and negative controls have been excluded. (B) Binding data for anti-Lewis X antibodies to Lewis X positive MCF7 cell line and Lewis X negative SK-MEL-28 as compared to positive control anti-Lewis X antibody MC-480. (C) Binding data for anti-Tn antibodies to Tn positive MCF7 cell line and Tn negative HEK-293 cell line as compared to positive control anti-Tn antibody SBH-Tn. Error bars (B,C) represent the SD of three experiments. Additional bar graphs can be found in Figure S8. Representative histograms and additional flow cytometry data can be found in Figure S9.

    Article Snippet: The human breast cancer cell line MCF7 was generously provided by the NCI Developmental Therapeutics Program and was authenticated by ATCC’s cell line authentication service (ATCC 135-XV).

    Techniques: Binding Assay, Fluorescence, Concentration Assay, Positive Control, Flow Cytometry