human brain derived pericytes  (Lonza)

Journal: IBRO Neuroscience Reports

Article Title: Brain pericytes upregulate glutamate uptake by astrocytes in vitro through sodium-dependent transporter

doi: 10.1016/j.ibneur.2025.05.017

Figure Lengend Snippet: Brain pericytes increase glutamate uptake in astrocyte cultures. (A) Double immunofluorescence staining for CD13/EAAT1 (top left panel), CD13/EAAT2 (top right panel), GFAP/EAAT1 (bottom left panel), and GFAP/EAAT2 (bottom right panel) in intact mouse hippocampus sections. CD13 and GFAP signals appear green, while EAAT1 and EAAT2 signals appear red. (B) Regression analyses of cell/medium ratios of [ 3 H]-L-glutamate ([ 3 H]-L-Glu) uptake versus incubation time in astrocytes cultured without pericytes (astrocyte monocultures) or with pericytes (pericyte co-cultures). Astrocytes in monocultures and pericyte co-cultures were incubated in uptake buffer containing [ 3 H]-L-Glu for 1, 2, 5, and 10 min. A linear regression analysis was also performed to obtain the rate of [ 3 H]-L-Glu uptake. The slope of the lines was 9.48 in astrocyte monocultures ( r 2 = 0.90) and 13.54 in pericyte co-cultures ( r 2 = 0.89). Values are expressed as means ± SEM (n = 4 per point). * p < 0.05, between astrocyte monocultures and pericyte co-cultures.

Article Snippet: Human brain-derived pericytes (#1200, Lot 27194; ScienCell Research Laboratories) and human brain-derived astrocytes (#CC-2565, Lot 0000647218; Lonza Bioscience) at passages 4–8 and 4–5, respectively, were used.

Techniques: Double Immunofluorescence Staining, Incubation, Cell Culture

Journal: IBRO Neuroscience Reports

Article Title: Brain pericytes upregulate glutamate uptake by astrocytes in vitro through sodium-dependent transporter

doi: 10.1016/j.ibneur.2025.05.017

Figure Lengend Snippet: Brain pericytes increase the Na + -dependent glutamate uptake in astrocytes. (A) Effect of Na + -free conditions on [ 3 H]-L-Glu uptake by astrocytes in monocultures or pericyte co-cultures. Results are expressed as the % of [ 3 H]-L-Glu uptake by astrocytes in monocultures incubated with uptake buffer containing Na + and [ 3 H]-L-Glu (Control; 113.96 ± 2.97 μL/mg protein). Values expressed are means ± SEM (n = 3); * p < 0.05, ** p < 0.01. (B) Effect of the EAAT1 inhibitor UCPH-101 (10 μM) on [ 3 H]-L-Glu uptake by astrocytes in monocultures or pericyte co-cultures. (C) Effect of the EAAT2 inhibitor DHK (500 μM) on [ 3 H]-L-Glu uptake by astrocytes in monocultures or pericyte co-cultures. Results are expressed as the % decrease from [ 3 H]-L-Glu uptake in astrocytes of the corresponding vehicle-treated group: 92.11 ± 12.34 μL/mg protein (B) and 75.32 ± 7.45 μL/mg protein (C) in astrocyte monocultures and 111.15 ± 14.40 μL/mg protein (B) and 98.42 ± 10.88 μL/mg protein (C) in pericyte co-cultures, respectively. Values expressed are the means ± SEM (n = 5–7); * p < 0.05.

Article Snippet: Human brain-derived pericytes (#1200, Lot 27194; ScienCell Research Laboratories) and human brain-derived astrocytes (#CC-2565, Lot 0000647218; Lonza Bioscience) at passages 4–8 and 4–5, respectively, were used.

Techniques: Incubation, Control

Journal: IBRO Neuroscience Reports

Article Title: Brain pericytes upregulate glutamate uptake by astrocytes in vitro through sodium-dependent transporter

doi: 10.1016/j.ibneur.2025.05.017

Figure Lengend Snippet: Effect of pericytes on the protein expression levels of EAAT1 and EAAT2 in astrocytes. Representative western blot images (top panels) and densitometric analyses (bottom panels) of the expression of EAAT1 (A) and EAAT2 (B) in astrocytes. The protein levels of EAAT1 and EAAT2 were normalized to those of GAPDH. Results are expressed as the % of expression in astrocyte monocultures. Values expressed are the means ± SEM (n = 3).

Article Snippet: Human brain-derived pericytes (#1200, Lot 27194; ScienCell Research Laboratories) and human brain-derived astrocytes (#CC-2565, Lot 0000647218; Lonza Bioscience) at passages 4–8 and 4–5, respectively, were used.

Techniques: Expressing, Western Blot

Journal: IBRO Neuroscience Reports

Article Title: Brain pericytes upregulate glutamate uptake by astrocytes in vitro through sodium-dependent transporter

doi: 10.1016/j.ibneur.2025.05.017

Figure Lengend Snippet: Treatment with pericyte-conditioned medium (pericyte-CM) upregulates glutamate uptake in astrocyte monocultures. Astrocyte monocultures exposed to culture medium (0 %), 50 % pericyte-CM, or 100 % pericyte-CM for 3 days were incubated with uptake buffer containing [ 3 H]-L-Glu for 10 min. Results are expressed as the % of [ 3 H]-L-Glu uptake by astrocytes in monocultures treated with 0 % pericyte-CM (72.35 ± 8.84 μL/mg protein). Values expressed are the means ± SEM (n = 3–6); ** p < 0.01.

Article Snippet: Human brain-derived pericytes (#1200, Lot 27194; ScienCell Research Laboratories) and human brain-derived astrocytes (#CC-2565, Lot 0000647218; Lonza Bioscience) at passages 4–8 and 4–5, respectively, were used.

Techniques: Incubation

Journal: IBRO Neuroscience Reports

Article Title: Brain pericytes upregulate glutamate uptake by astrocytes in vitro through sodium-dependent transporter

doi: 10.1016/j.ibneur.2025.05.017

Figure Lengend Snippet: Brain pericytes increase glutamate uptake in astrocyte cultures. (A) Double immunofluorescence staining for CD13/EAAT1 (top left panel), CD13/EAAT2 (top right panel), GFAP/EAAT1 (bottom left panel), and GFAP/EAAT2 (bottom right panel) in intact mouse hippocampus sections. CD13 and GFAP signals appear green, while EAAT1 and EAAT2 signals appear red. (B) Regression analyses of cell/medium ratios of [ 3 H]-L-glutamate ([ 3 H]-L-Glu) uptake versus incubation time in astrocytes cultured without pericytes (astrocyte monocultures) or with pericytes (pericyte co-cultures). Astrocytes in monocultures and pericyte co-cultures were incubated in uptake buffer containing [ 3 H]-L-Glu for 1, 2, 5, and 10 min. A linear regression analysis was also performed to obtain the rate of [ 3 H]-L-Glu uptake. The slope of the lines was 9.48 in astrocyte monocultures ( r 2 = 0.90) and 13.54 in pericyte co-cultures ( r 2 = 0.89). Values are expressed as means ± SEM (n = 4 per point). * p < 0.05, between astrocyte monocultures and pericyte co-cultures.

Article Snippet: Human brain-derived pericytes (#1200, Lot 27194; ScienCell Research Laboratories) and human brain-derived astrocytes (#CC-2565, Lot 0000647218; Lonza Bioscience) at passages 4–8 and 4–5, respectively, were used.

Techniques: Double Immunofluorescence Staining, Incubation, Cell Culture

Journal: IBRO Neuroscience Reports

Article Title: Brain pericytes upregulate glutamate uptake by astrocytes in vitro through sodium-dependent transporter

doi: 10.1016/j.ibneur.2025.05.017

Figure Lengend Snippet: Brain pericytes increase the Na + -dependent glutamate uptake in astrocytes. (A) Effect of Na + -free conditions on [ 3 H]-L-Glu uptake by astrocytes in monocultures or pericyte co-cultures. Results are expressed as the % of [ 3 H]-L-Glu uptake by astrocytes in monocultures incubated with uptake buffer containing Na + and [ 3 H]-L-Glu (Control; 113.96 ± 2.97 μL/mg protein). Values expressed are means ± SEM (n = 3); * p < 0.05, ** p < 0.01. (B) Effect of the EAAT1 inhibitor UCPH-101 (10 μM) on [ 3 H]-L-Glu uptake by astrocytes in monocultures or pericyte co-cultures. (C) Effect of the EAAT2 inhibitor DHK (500 μM) on [ 3 H]-L-Glu uptake by astrocytes in monocultures or pericyte co-cultures. Results are expressed as the % decrease from [ 3 H]-L-Glu uptake in astrocytes of the corresponding vehicle-treated group: 92.11 ± 12.34 μL/mg protein (B) and 75.32 ± 7.45 μL/mg protein (C) in astrocyte monocultures and 111.15 ± 14.40 μL/mg protein (B) and 98.42 ± 10.88 μL/mg protein (C) in pericyte co-cultures, respectively. Values expressed are the means ± SEM (n = 5–7); * p < 0.05.

Article Snippet: Human brain-derived pericytes (#1200, Lot 27194; ScienCell Research Laboratories) and human brain-derived astrocytes (#CC-2565, Lot 0000647218; Lonza Bioscience) at passages 4–8 and 4–5, respectively, were used.

Techniques: Incubation, Control

Journal: IBRO Neuroscience Reports

Article Title: Brain pericytes upregulate glutamate uptake by astrocytes in vitro through sodium-dependent transporter

doi: 10.1016/j.ibneur.2025.05.017

Figure Lengend Snippet: Effect of pericytes on the protein expression levels of EAAT1 and EAAT2 in astrocytes. Representative western blot images (top panels) and densitometric analyses (bottom panels) of the expression of EAAT1 (A) and EAAT2 (B) in astrocytes. The protein levels of EAAT1 and EAAT2 were normalized to those of GAPDH. Results are expressed as the % of expression in astrocyte monocultures. Values expressed are the means ± SEM (n = 3).

Article Snippet: Human brain-derived pericytes (#1200, Lot 27194; ScienCell Research Laboratories) and human brain-derived astrocytes (#CC-2565, Lot 0000647218; Lonza Bioscience) at passages 4–8 and 4–5, respectively, were used.

Techniques: Expressing, Western Blot

Journal: IBRO Neuroscience Reports

Article Title: Brain pericytes upregulate glutamate uptake by astrocytes in vitro through sodium-dependent transporter

doi: 10.1016/j.ibneur.2025.05.017

Figure Lengend Snippet: Treatment with pericyte-conditioned medium (pericyte-CM) upregulates glutamate uptake in astrocyte monocultures. Astrocyte monocultures exposed to culture medium (0 %), 50 % pericyte-CM, or 100 % pericyte-CM for 3 days were incubated with uptake buffer containing [ 3 H]-L-Glu for 10 min. Results are expressed as the % of [ 3 H]-L-Glu uptake by astrocytes in monocultures treated with 0 % pericyte-CM (72.35 ± 8.84 μL/mg protein). Values expressed are the means ± SEM (n = 3–6); ** p < 0.01.

Article Snippet: Human brain-derived pericytes (#1200, Lot 27194; ScienCell Research Laboratories) and human brain-derived astrocytes (#CC-2565, Lot 0000647218; Lonza Bioscience) at passages 4–8 and 4–5, respectively, were used.

Techniques: Incubation