Journal: International Journal of Nanomedicine

Article Title: Using poly(lactic-co-glycolic acid) microspheres to encapsulate plasmid of bone morphogenetic protein 2/polyethylenimine nanoparticles to promote bone formation in vitro and in vivo

doi: 10.2147/IJN.S45184

Figure Lengend Snippet: Physical characteristics of pBMP-2/PEI nanoparticles, morphology of PLGA@pBMP-2/PEI, and release assay of pBMP-2. ( A ) Size distribution of BMP-2/PEI nanoparticles; ( B ) Zeta potential of BMP-2/PEI nanoparticles; ( C ) Scanning electron microscope image of PLGA@pBMP-2/PEI; ( D ) Fluorescence image of PLGA@pBMP-2/PEI/FITC; ( E ) pBMP-2 release profile from PLGA@pBMP-2/PEI in PBS; ( F ) PCR assays to confirm pBMP-2 releasing into the PBS buffer. Lane 1: DNA marker. Lane 2: PCR amplicon of original pBMP-2 plasmid. Lane 3: PCR amplicon from day 7 PBS buffer Abbreviations: PLGA@pBMP-2/PEI/FITC, poly(lactic-co-glycolic acid)@plasmid of bone morphogenetic protein 2/polyethlenimine/fluorescein isothiocyanate; bp, base pair; PCR, polymerase chain reaction; PBS, phosphate buffered salin.

Article Snippet: Human BMP-2 concentration was determined with a BMP-2 ELISA kit (BOSTER, Wuhan, People’s Republic of China).

Techniques: Release Assay, Microscopy, Fluorescence, Marker, Amplification, Plasmid Preparation, Polymerase Chain Reaction

Journal: International Journal of Nanomedicine

Article Title: Using poly(lactic-co-glycolic acid) microspheres to encapsulate plasmid of bone morphogenetic protein 2/polyethylenimine nanoparticles to promote bone formation in vitro and in vivo

doi: 10.2147/IJN.S45184

Figure Lengend Snippet: Evaluate properties of PLGA@pBMP-2/PEI in vitro. ( A ) Transfection ability of PLGA@pBMP-2/PEI/FITC in MC3T3-E1 cells at day 3; ( B ) Transfection ability of PLGA@ pBMP-2/PEI/FITC in MC3T3 cells at day 7; ( C ) PCR assays to confirm pBMP-2 in MC3T3 cells. Lane 1: DNA marker; Lane 2: PCR amplicon from PLGA@pBMP-2/PEI transfected MC3T3 cells; Lane 3: PCR from MC3T3 cells; ( D ) Human BMP-2 ELISA assays to confirm human BMP-2 secretion from transfected MC3T3 cells. ( E ) Calcium deposition analysis of PLGA@PEI group on day 14 using Alizarin red staining; ( F ) Calcium deposition analysis of PLGA@pBMP-2/PEI group on day 14 using Alizarin red staining. Note: *significant difference ( p <0.01). Abbreviations: PLGA@PEI, poly(lactic-co-glycolic acid)@polyethlenimine; PLGA@pBMP-2/PEI, poly(lactic-co-glycolic acid)@plasmid of bone morphogenetic protein 2/polyethlenimine; PLGA@pBMP-2/PEI/FITC, poly(lactic-co-glycolic acid)@plasmid of bone morphogenetic protein 2/polyethlenimine/fluorescein isothiocyanate; bp, base pair; PCR, polymerase chain reaction; ELISA, enzyme-linked immunosorbent assay.

Article Snippet: Human BMP-2 concentration was determined with a BMP-2 ELISA kit (BOSTER, Wuhan, People’s Republic of China).

Techniques: In Vitro, Transfection, Marker, Amplification, Enzyme-linked Immunosorbent Assay, Staining, Plasmid Preparation, Polymerase Chain Reaction

Journal: International Journal of Nanomedicine

Article Title: Using poly(lactic-co-glycolic acid) microspheres to encapsulate plasmid of bone morphogenetic protein 2/polyethylenimine nanoparticles to promote bone formation in vitro and in vivo

doi: 10.2147/IJN.S45184

Figure Lengend Snippet: H&E staining and immunohistochemistry staining after 4 or 8 weeks postimplantation. ( A ) H&E staining of non-implant control after 4 weeks (×40); ( B ) H&E staining of non-implant control after 8 weeks (×40); ( C ) H&E staining of PLGA@ PEI group after 4 weeks (×40); ( D ) H&E staining of PLGA@PEI group after 8 weeks (×40); ( E ) H&E staining of PLGA@pBMP-2/PEI group after 4 weeks (×40), red arrow indicates new bone; ( F ) H&E staining of PLGA/pBMP-2/PEI group after 8 weeks (×40), red arrows indicate new bone; ( G ) Enlarged image of new bone area from . Red arrows indicate osteoblasts and yellow arrow indicates a macrophages phagocytized foreign materials; ( H ) Enlarged image of new bone area from . Red arrow indicates osteoblasts, and yellow arrow indicates a macrophages phagocytized foreign materials; ( I ) H&E staining of PLGA@pBMP-2/PEI group after 4 weeks (×200). OB: original bone (or lamellar bone); NB: new bone (or woven bone); Yellow arrow indicates the edge of the defect area; ( J ) H&E staining of PLGA@pBMP-2/PEI group after 8 weeks (×200). Yellow arrow indicates the edge of the defect area; ( K ) Human BMP-2 immunohistochemistry staining of PLGA@PEI group; ( L ) Human BMP-2 immunohistochemistry staining of PLGA/pBMP-2/PEI group. Abbreviations: H&E, hematoxylin and eosin stain; PLGA@PEI, poly(lactic-co-glycolic acid)@polyethlenimine; PLGA@pBMP-2/PEI, poly(lactic-co-glycolic acid)@plasmid of bone morphogenetic protein 2/polyethlenimine;

Article Snippet: Human BMP-2 concentration was determined with a BMP-2 ELISA kit (BOSTER, Wuhan, People’s Republic of China).

Techniques: Staining, Immunohistochemistry, H&E Stain, Plasmid Preparation

Journal: Turkish Journal of Biology

Article Title: The promising effects of BMP2 transfected mesenchymal stem cells on human osteosarcoma

doi: 10.3906/biy-2101-50

Figure Lengend Snippet: Primary antibodies used in immunohistochemical studies.

Article Snippet: The BMP2 levels in hMSCs and BMP2 + hMSCs cultures, and 143B+hMSCs and 143B+BMP2 + hMSCs cocultures were measured (×3 for each sample) in the 4th and 14th culture days using human BMP2 ELISA kit (#EK0311, Boster, Pleasanton, CA, USA) according to the manufacturer’s instructions.

Techniques: Immunohistochemical staining

Journal: Turkish Journal of Biology

Article Title: The promising effects of BMP2 transfected mesenchymal stem cells on human osteosarcoma

doi: 10.3906/biy-2101-50

Figure Lengend Snippet: Primer sequences used in RT-PCR.

Article Snippet: The BMP2 levels in hMSCs and BMP2 + hMSCs cultures, and 143B+hMSCs and 143B+BMP2 + hMSCs cocultures were measured (×3 for each sample) in the 4th and 14th culture days using human BMP2 ELISA kit (#EK0311, Boster, Pleasanton, CA, USA) according to the manufacturer’s instructions.

Techniques: