human bc cell lines mda mb 231  (ATCC)


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    ATCC human bc cell lines mda mb 231
    (A) Flow chart illustrating the steps for miRNA expression microarray analysis using AffymetrixGeneChip ® . (B) Heat maps with hierarchical clustering showing the differential expression of miRNAs in <t>MM231</t> wild-type , MM231 resistant-type , MM468 wild-type , MM468 resistant-type , HCC1395 wild-type , and HCC1395 resistant-type BC cell lines. (C) Venn diagrams illustrating the number of all upregulated and downregulated DEMs in three pairs of BC cell lines. The intersection in the center represents the common DEMs among the three groups. miRNA or miR, microRNA; BC, breast cancer; DEMs, differentially expressed miRNAs.
    Human Bc Cell Lines Mda Mb 231, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Exploring miRNA‑target gene profiles associated with drug resistance in patients with breast cancer receiving neoadjuvant chemotherapy"

    Article Title: Exploring miRNA‑target gene profiles associated with drug resistance in patients with breast cancer receiving neoadjuvant chemotherapy

    Journal: Oncology Letters

    doi: 10.3892/ol.2024.14291

    (A) Flow chart illustrating the steps for miRNA expression microarray analysis using AffymetrixGeneChip ® . (B) Heat maps with hierarchical clustering showing the differential expression of miRNAs in MM231 wild-type , MM231 resistant-type , MM468 wild-type , MM468 resistant-type , HCC1395 wild-type , and HCC1395 resistant-type BC cell lines. (C) Venn diagrams illustrating the number of all upregulated and downregulated DEMs in three pairs of BC cell lines. The intersection in the center represents the common DEMs among the three groups. miRNA or miR, microRNA; BC, breast cancer; DEMs, differentially expressed miRNAs.
    Figure Legend Snippet: (A) Flow chart illustrating the steps for miRNA expression microarray analysis using AffymetrixGeneChip ® . (B) Heat maps with hierarchical clustering showing the differential expression of miRNAs in MM231 wild-type , MM231 resistant-type , MM468 wild-type , MM468 resistant-type , HCC1395 wild-type , and HCC1395 resistant-type BC cell lines. (C) Venn diagrams illustrating the number of all upregulated and downregulated DEMs in three pairs of BC cell lines. The intersection in the center represents the common DEMs among the three groups. miRNA or miR, microRNA; BC, breast cancer; DEMs, differentially expressed miRNAs.

    Techniques Used: Expressing, Microarray

    (A) Venn diagram showing candidate exosomal miRNAs related to drug resistance. Bar charts showing fold-change in exosomal miRNAs from wild-type and resistant-type cells. A total of 12 selected candidate exosomal miRNAs were analyzed in the three different breast cancer cell lines (B) MM231, (C) MM468, and (D) HCC1395, using quantitative PCR. Significant miRNAs are indicated in bold text. miRNA or miR, microRNA.
    Figure Legend Snippet: (A) Venn diagram showing candidate exosomal miRNAs related to drug resistance. Bar charts showing fold-change in exosomal miRNAs from wild-type and resistant-type cells. A total of 12 selected candidate exosomal miRNAs were analyzed in the three different breast cancer cell lines (B) MM231, (C) MM468, and (D) HCC1395, using quantitative PCR. Significant miRNAs are indicated in bold text. miRNA or miR, microRNA.

    Techniques Used: Real-time Polymerase Chain Reaction

    (A) Radar plots of miRNA signature profiles from quantitative PCR in BC cells, including MDA-MB-231 (MM231), MDA-MB-468 (MM468), and HCC1395. The relative expression levels of miRNAs in wild-type BC cells, drug-resistant exosome 24 h-treated (educated-type) BC cells, and resistant-type BC cells were compared. The line labels represent the drug-resistant miRNAs, and the ring labels represent the fold change calculated from each group/wild-type difference. (B) The cell viabilities of wild-type, educated-type, and resistant-type BC cells were compared after 48 h of incubation with Adriamycin and Taxotere. miRNA or miR, microRNA; BC, breast cancer; ADR, Adriamycin; TAX, Taxotere.
    Figure Legend Snippet: (A) Radar plots of miRNA signature profiles from quantitative PCR in BC cells, including MDA-MB-231 (MM231), MDA-MB-468 (MM468), and HCC1395. The relative expression levels of miRNAs in wild-type BC cells, drug-resistant exosome 24 h-treated (educated-type) BC cells, and resistant-type BC cells were compared. The line labels represent the drug-resistant miRNAs, and the ring labels represent the fold change calculated from each group/wild-type difference. (B) The cell viabilities of wild-type, educated-type, and resistant-type BC cells were compared after 48 h of incubation with Adriamycin and Taxotere. miRNA or miR, microRNA; BC, breast cancer; ADR, Adriamycin; TAX, Taxotere.

    Techniques Used: Real-time Polymerase Chain Reaction, Expressing, Incubation

    human bc cell lines mda mb 231  (ATCC)


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    ATCC human bc cell lines mda mb 231
    A Volcano plot showed that 13,582 LncRNAs were differential expression by using lncRNA expression thresholds of more than twofold change with P < 0.05 in TCGA. (no -DEGs: no difference, UP: upregulation, Down: downregulation). B The Linc00707 expression in TNBC and Non-TNBC was obtained from TCGA database. C The Linc00707 expression in TNBC, normal breast tissues (Normal), Her2 overexpressing breast cancer tissues (Her2+), Luminal A (LumA) and Luminal B (LumB) was obtained from TCGA database. D qRT-PCR analyses of Linc00707 expression levels in TNBC specimens compared with ANT and Non-TNBC. E ROC curve for diagnosis of TNBC by Linc00707 level in tissues. F The expression of Linc00707 in MCF-10A and different breast cancer cell lines. G The expression level of Linc00707 in the subcellular <t>fractions</t> <t>of</t> <t>MDA-MB-231</t> and MDA-MB-468 cells used by RT-qPCR. U6 and GAPDH were used as nuclear and cytoplasmic markers, respectively. H The expression of Linc00707 in MDA-MB-231 and MDA-MB-468 cells by RNA FISH. Nuclei were stained with DAPI (blue) and Linc00707 probes were labeled with Cy3 (red). U6 RNA were used as positive controls of in the nucleus and 18 S were used as positive controls of in the cytoplasm. Scale bar, 50 μm. All experiments were repeated independently three times. Data are presented as means ± standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001.
    Human Bc Cell Lines Mda Mb 231, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Linc00707 regulates autophagy and promotes the progression of triple negative breast cancer by activation of PI3K/AKT/mTOR pathway"

    Article Title: Linc00707 regulates autophagy and promotes the progression of triple negative breast cancer by activation of PI3K/AKT/mTOR pathway

    Journal: Cell Death Discovery

    doi: 10.1038/s41420-024-01906-7

    A Volcano plot showed that 13,582 LncRNAs were differential expression by using lncRNA expression thresholds of more than twofold change with P < 0.05 in TCGA. (no -DEGs: no difference, UP: upregulation, Down: downregulation). B The Linc00707 expression in TNBC and Non-TNBC was obtained from TCGA database. C The Linc00707 expression in TNBC, normal breast tissues (Normal), Her2 overexpressing breast cancer tissues (Her2+), Luminal A (LumA) and Luminal B (LumB) was obtained from TCGA database. D qRT-PCR analyses of Linc00707 expression levels in TNBC specimens compared with ANT and Non-TNBC. E ROC curve for diagnosis of TNBC by Linc00707 level in tissues. F The expression of Linc00707 in MCF-10A and different breast cancer cell lines. G The expression level of Linc00707 in the subcellular fractions of MDA-MB-231 and MDA-MB-468 cells used by RT-qPCR. U6 and GAPDH were used as nuclear and cytoplasmic markers, respectively. H The expression of Linc00707 in MDA-MB-231 and MDA-MB-468 cells by RNA FISH. Nuclei were stained with DAPI (blue) and Linc00707 probes were labeled with Cy3 (red). U6 RNA were used as positive controls of in the nucleus and 18 S were used as positive controls of in the cytoplasm. Scale bar, 50 μm. All experiments were repeated independently three times. Data are presented as means ± standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001.
    Figure Legend Snippet: A Volcano plot showed that 13,582 LncRNAs were differential expression by using lncRNA expression thresholds of more than twofold change with P < 0.05 in TCGA. (no -DEGs: no difference, UP: upregulation, Down: downregulation). B The Linc00707 expression in TNBC and Non-TNBC was obtained from TCGA database. C The Linc00707 expression in TNBC, normal breast tissues (Normal), Her2 overexpressing breast cancer tissues (Her2+), Luminal A (LumA) and Luminal B (LumB) was obtained from TCGA database. D qRT-PCR analyses of Linc00707 expression levels in TNBC specimens compared with ANT and Non-TNBC. E ROC curve for diagnosis of TNBC by Linc00707 level in tissues. F The expression of Linc00707 in MCF-10A and different breast cancer cell lines. G The expression level of Linc00707 in the subcellular fractions of MDA-MB-231 and MDA-MB-468 cells used by RT-qPCR. U6 and GAPDH were used as nuclear and cytoplasmic markers, respectively. H The expression of Linc00707 in MDA-MB-231 and MDA-MB-468 cells by RNA FISH. Nuclei were stained with DAPI (blue) and Linc00707 probes were labeled with Cy3 (red). U6 RNA were used as positive controls of in the nucleus and 18 S were used as positive controls of in the cytoplasm. Scale bar, 50 μm. All experiments were repeated independently three times. Data are presented as means ± standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001.

    Techniques Used: Expressing, Quantitative RT-PCR, Staining, Labeling, Standard Deviation

    A , B Analysis of Linc00707 expression after siRNAs transfection in MDA-MB-231 and MDA-MB-468 cells by RT-qPCR. C , D Analysis of Linc00707 expression after overexpression vector transfection in MDA-MB-231 and MDA-MB-468 cells by RT-qPCR. E EdU assay of the cell proliferation ability in MDA-MB-231 cells. Scale bar: 100 μm. F EdU assay of the cell proliferation ability in MDA-MB-468 cells. Scale bar: 100 μm. G The nude mice were subcutaneously injected with 5 × 10 6 MDA-MB-231 cells stably transfected with LV-sh-Linc00707 or LV-sh-con cells. A ruler was used to indicate the size of the tumors. The tumor weight with different cells was shown. H Immunohistochemistry for Ki-67 detection in LV-sh-con and LV-sh-Linc00707 group. I , J Transwell assay in MDA-MB-231 and MDA-MB-468 cells transfected with sh-con or sh-Linc00707 #1 or sh-Linc00707 #2, oe-NC or oe-Linc00707. Scale bar: 100 μm. K Representative images of the lung obtained from nude mice and numbers of lung metastasis lesions were calculated. L HE staining of lung tissues were used to detect the metastasis nodules. All experiments were repeated independently three times. Data are presented as means ± standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001.
    Figure Legend Snippet: A , B Analysis of Linc00707 expression after siRNAs transfection in MDA-MB-231 and MDA-MB-468 cells by RT-qPCR. C , D Analysis of Linc00707 expression after overexpression vector transfection in MDA-MB-231 and MDA-MB-468 cells by RT-qPCR. E EdU assay of the cell proliferation ability in MDA-MB-231 cells. Scale bar: 100 μm. F EdU assay of the cell proliferation ability in MDA-MB-468 cells. Scale bar: 100 μm. G The nude mice were subcutaneously injected with 5 × 10 6 MDA-MB-231 cells stably transfected with LV-sh-Linc00707 or LV-sh-con cells. A ruler was used to indicate the size of the tumors. The tumor weight with different cells was shown. H Immunohistochemistry for Ki-67 detection in LV-sh-con and LV-sh-Linc00707 group. I , J Transwell assay in MDA-MB-231 and MDA-MB-468 cells transfected with sh-con or sh-Linc00707 #1 or sh-Linc00707 #2, oe-NC or oe-Linc00707. Scale bar: 100 μm. K Representative images of the lung obtained from nude mice and numbers of lung metastasis lesions were calculated. L HE staining of lung tissues were used to detect the metastasis nodules. All experiments were repeated independently three times. Data are presented as means ± standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001.

    Techniques Used: Expressing, Transfection, Quantitative RT-PCR, Over Expression, Plasmid Preparation, EdU Assay, Injection, Stable Transfection, Immunohistochemistry, Transwell Assay, Staining, Standard Deviation

    A The MDA-MB-231 and MDA-MB-468 cell lines stably expressing stubRFP-sensGFP-LC3 were transfected with sh-con and sh-Linc00707 plasmids were observed by the fluorescence microscope, and the StubRFP-SensGFP-LC3 fluorescence spots were photographed under laser confocal microscopy. GFP represented autophagosomes, RFP represented the total number of autophagosomes and autolysosomes, yellow puncta in the merged image represented the flow rate of autophagic flow from autophagosomes to autolysosomes. Scale bar: 50 μm. B The changes of mitochondrial membrane potential of sh-con and sh-linc00707 groups in MDA-MB-231 and MDA-MB-468 were monitored by JC-1. Green fluorescence: JC-1 monomer, red fluorescence: JC-1 aggregates. Scale bar: 50 μm. C Western Blot analysis of the ratio of LC3-II/LC3-I and protein levels of SQSTM1/P62 in MDA-MB-231 and MDA-MB-468 cells transfected with sh-con or sh-Linc00707, oe-NC or oe-Linc00707. Western blot results are expressed as fold changes in relative band densities to control from three independent experiments. D Western Blot analysis of protein levels of p-PI3K, PI3K, p-AKT, AKT, p-mTOR, mTOR in MDA-MB-231 and MDA-MB-468 cells transfected with sh-con or sh-Linc00707, oe-NC or oe-Linc00707. Western blot results are expressed as fold changes in relative band densities to control from three independent experiments. E Western Blot analysis of protein levels of p-p70s6k, p70s6k, p-4EBP1, 4EBP1 in MDA-MB-231 and MDA-MB-468 cells transfected with sh-con or sh-Linc00707, oe-NC or oe-Linc00707. Western blot results are expressed as fold changes in relative band densities to control from three independent experiments. All experiments were repeated independently three times. Data are presented as means ± standard deviation. * P < 0.05.
    Figure Legend Snippet: A The MDA-MB-231 and MDA-MB-468 cell lines stably expressing stubRFP-sensGFP-LC3 were transfected with sh-con and sh-Linc00707 plasmids were observed by the fluorescence microscope, and the StubRFP-SensGFP-LC3 fluorescence spots were photographed under laser confocal microscopy. GFP represented autophagosomes, RFP represented the total number of autophagosomes and autolysosomes, yellow puncta in the merged image represented the flow rate of autophagic flow from autophagosomes to autolysosomes. Scale bar: 50 μm. B The changes of mitochondrial membrane potential of sh-con and sh-linc00707 groups in MDA-MB-231 and MDA-MB-468 were monitored by JC-1. Green fluorescence: JC-1 monomer, red fluorescence: JC-1 aggregates. Scale bar: 50 μm. C Western Blot analysis of the ratio of LC3-II/LC3-I and protein levels of SQSTM1/P62 in MDA-MB-231 and MDA-MB-468 cells transfected with sh-con or sh-Linc00707, oe-NC or oe-Linc00707. Western blot results are expressed as fold changes in relative band densities to control from three independent experiments. D Western Blot analysis of protein levels of p-PI3K, PI3K, p-AKT, AKT, p-mTOR, mTOR in MDA-MB-231 and MDA-MB-468 cells transfected with sh-con or sh-Linc00707, oe-NC or oe-Linc00707. Western blot results are expressed as fold changes in relative band densities to control from three independent experiments. E Western Blot analysis of protein levels of p-p70s6k, p70s6k, p-4EBP1, 4EBP1 in MDA-MB-231 and MDA-MB-468 cells transfected with sh-con or sh-Linc00707, oe-NC or oe-Linc00707. Western blot results are expressed as fold changes in relative band densities to control from three independent experiments. All experiments were repeated independently three times. Data are presented as means ± standard deviation. * P < 0.05.

    Techniques Used: Stable Transfection, Expressing, Transfection, Fluorescence, Microscopy, Confocal Microscopy, Membrane, Western Blot, Standard Deviation

    A , B EdU analysis of the cell proliferation capacity in MDA-MB-231 and MDA-MB-468 cells transfected with sh-con, sh-Linc00707 #1, sh-Linc00707 #2, oe-NC or oe-Linc00707 after CQ treatment. Scale bar: 100 μm. C , D Transwell assay analysis of the cell invasion and migration capacity in MDA-MB-231 and MDA-MB-468 cells transfected with sh-con, sh-Linc00707 #1, sh-Linc00707 #2, oe-NC or oe-Linc00707 after CQ treatment. Scale bar: 100 μm. E , F Wound healing assay analysis of the cell migration capacity in MDA-MB-231 and MDA-MB-468 cells transfected with sh-con, sh-Linc00707 #1, sh-Linc00707 #2, oe-NC or oe-Linc00707 after CQ treatment. Scale bar: 500 μm. All experiments were repeated independently three times. Data are presented as means ± standard deviation. * P < 0.05, ** P < 0.01, *** P < 0.001.
    Figure Legend Snippet: A , B EdU analysis of the cell proliferation capacity in MDA-MB-231 and MDA-MB-468 cells transfected with sh-con, sh-Linc00707 #1, sh-Linc00707 #2, oe-NC or oe-Linc00707 after CQ treatment. Scale bar: 100 μm. C , D Transwell assay analysis of the cell invasion and migration capacity in MDA-MB-231 and MDA-MB-468 cells transfected with sh-con, sh-Linc00707 #1, sh-Linc00707 #2, oe-NC or oe-Linc00707 after CQ treatment. Scale bar: 100 μm. E , F Wound healing assay analysis of the cell migration capacity in MDA-MB-231 and MDA-MB-468 cells transfected with sh-con, sh-Linc00707 #1, sh-Linc00707 #2, oe-NC or oe-Linc00707 after CQ treatment. Scale bar: 500 μm. All experiments were repeated independently three times. Data are presented as means ± standard deviation. * P < 0.05, ** P < 0.01, *** P < 0.001.

    Techniques Used: Transfection, Transwell Assay, Migration, Wound Healing Assay, Standard Deviation

    A The Venn diagram of miRNAs binding to Linc00707 were predicted using by using online database Starbase v.2.0 and LncBase v.2.0. B , C qRT-PCR analyses of the relative levels of 9 miRNA candidates in sh-Linc00707 and sh-con of MDA-MB-231 and MDA-MB-468. D Relative Linc00707 level in MDA-MB-231 and MDA-MB-468 cells lysates captured by biotin-labeled miR-423-5p or miR-NC was detected by RT-qPCR. E Binding sites of Linc00707 and miR-423-5p. F Relative luciferase activities in HEK293T cells co-transfected with Linc00707-WT or Linc00707-MUT and miR-423 overexpression vector (miR-423 mimics) or miR-NC. G , H Anti-AGO2 RIP was performed using MDA-MB-231 and MDA-MB-468 cells followed by RT-qPCR to detect Linc00707 and miR-423-5p. I Pearson correlation analysis of Linc00707 expression and miR-423-5p level in 25 TNBC samples. All experiments were repeated independently three times. Data are presented as means ± standard deviation. ns P ≥ 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001.
    Figure Legend Snippet: A The Venn diagram of miRNAs binding to Linc00707 were predicted using by using online database Starbase v.2.0 and LncBase v.2.0. B , C qRT-PCR analyses of the relative levels of 9 miRNA candidates in sh-Linc00707 and sh-con of MDA-MB-231 and MDA-MB-468. D Relative Linc00707 level in MDA-MB-231 and MDA-MB-468 cells lysates captured by biotin-labeled miR-423-5p or miR-NC was detected by RT-qPCR. E Binding sites of Linc00707 and miR-423-5p. F Relative luciferase activities in HEK293T cells co-transfected with Linc00707-WT or Linc00707-MUT and miR-423 overexpression vector (miR-423 mimics) or miR-NC. G , H Anti-AGO2 RIP was performed using MDA-MB-231 and MDA-MB-468 cells followed by RT-qPCR to detect Linc00707 and miR-423-5p. I Pearson correlation analysis of Linc00707 expression and miR-423-5p level in 25 TNBC samples. All experiments were repeated independently three times. Data are presented as means ± standard deviation. ns P ≥ 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001.

    Techniques Used: Binding Assay, Quantitative RT-PCR, Labeling, Luciferase, Transfection, Over Expression, Plasmid Preparation, Expressing, Standard Deviation

    A , B Typical images of of the cell proliferation ability in MDA-MB-231 and MDA-MB-468 cells transfected with sh-con+miR-NC, sh-Linc00707 #1+miR-NC, sh-Linc00707 #2+miR-NC, sh-con+miR-423-5p inhibitor, sh-Linc00707 #1+miR-423-5p inhibitor, sh-Linc00707 #2+miR-423-5p inhibitor by EdU assay. Scale bar: 100 μm. C EdU statistical analysis of the cell proliferation ability in MDA-MB-231 and MDA-MB-468 cells. D Typical images of Transwell assay in MDA-MB-231 cells transfected with sh-con+miR-NC, sh-Linc00707 #1+miR-NC, sh-Linc00707 #2+miR-NC, sh-con+miR-423-5p inhibitor, sh-Linc00707 #1+miR-423-5p inhibitor, sh-Linc00707 #2+miR-423-5p inhibitor. Scale bar: 100 μm. E , F Transwell statistical analysis of the cell migration and invasion ability in MDA-MB-231 and MDA-MB-468 cells. G Typical images of Transwell assay in MDA-MB-468 cells transfected with sh-con+miR-NC, sh-Linc00707 #1+miR-NC, sh-Linc00707 #2+miR-NC, sh-con+miR-423-5p inhibitor, sh-Linc00707 #1+miR-423-5p inhibitor, sh-Linc00707 #2+miR-423-5p inhibitor. Scale bar: 100μm. All experiments were repeated independently three times. Data are presented as means ± standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001.
    Figure Legend Snippet: A , B Typical images of of the cell proliferation ability in MDA-MB-231 and MDA-MB-468 cells transfected with sh-con+miR-NC, sh-Linc00707 #1+miR-NC, sh-Linc00707 #2+miR-NC, sh-con+miR-423-5p inhibitor, sh-Linc00707 #1+miR-423-5p inhibitor, sh-Linc00707 #2+miR-423-5p inhibitor by EdU assay. Scale bar: 100 μm. C EdU statistical analysis of the cell proliferation ability in MDA-MB-231 and MDA-MB-468 cells. D Typical images of Transwell assay in MDA-MB-231 cells transfected with sh-con+miR-NC, sh-Linc00707 #1+miR-NC, sh-Linc00707 #2+miR-NC, sh-con+miR-423-5p inhibitor, sh-Linc00707 #1+miR-423-5p inhibitor, sh-Linc00707 #2+miR-423-5p inhibitor. Scale bar: 100 μm. E , F Transwell statistical analysis of the cell migration and invasion ability in MDA-MB-231 and MDA-MB-468 cells. G Typical images of Transwell assay in MDA-MB-468 cells transfected with sh-con+miR-NC, sh-Linc00707 #1+miR-NC, sh-Linc00707 #2+miR-NC, sh-con+miR-423-5p inhibitor, sh-Linc00707 #1+miR-423-5p inhibitor, sh-Linc00707 #2+miR-423-5p inhibitor. Scale bar: 100μm. All experiments were repeated independently three times. Data are presented as means ± standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001.

    Techniques Used: Transfection, EdU Assay, Transwell Assay, Migration, Standard Deviation

    A Binding sites of miR-423-5p and MARCH2. B The relative luciferase activities in HEK293T cells co-transfected with MARCH2-WT or MARCH2-MUT and miR-423 overexpression vector (miR-423 mimic) or miR-NC. C Western blot analysis of the protein expression level of MARCH2 in MDA-MB-231 and MDA-MB-468 cells. Western blot results are expressed as fold changes in relative band densities to control from three independent experiments. D Western blot analysis of the protein expression level of MARCH2, LC3-II/LC3-I, SQSTM1/P62,p-PI3K, PI3K, p-AKT, AKT, p-mTOR, mTOR in MDA-MB-231 and MDA-MB-468 cells with different cotransfection. Western blot results are expressed as fold changes in relative band densities to control from three independent experiments. E Schematic representation of the mechanism showed that Linc00707/miR-423-5p/MARCH2 regulates autophagy and progression of TNBC cells via the PI3K/AKT/mTOR pathway. All experiments were repeated independently three times. Data are presented as means ± standard deviation. ns P ≥ 0.05, **P < 0.01.
    Figure Legend Snippet: A Binding sites of miR-423-5p and MARCH2. B The relative luciferase activities in HEK293T cells co-transfected with MARCH2-WT or MARCH2-MUT and miR-423 overexpression vector (miR-423 mimic) or miR-NC. C Western blot analysis of the protein expression level of MARCH2 in MDA-MB-231 and MDA-MB-468 cells. Western blot results are expressed as fold changes in relative band densities to control from three independent experiments. D Western blot analysis of the protein expression level of MARCH2, LC3-II/LC3-I, SQSTM1/P62,p-PI3K, PI3K, p-AKT, AKT, p-mTOR, mTOR in MDA-MB-231 and MDA-MB-468 cells with different cotransfection. Western blot results are expressed as fold changes in relative band densities to control from three independent experiments. E Schematic representation of the mechanism showed that Linc00707/miR-423-5p/MARCH2 regulates autophagy and progression of TNBC cells via the PI3K/AKT/mTOR pathway. All experiments were repeated independently three times. Data are presented as means ± standard deviation. ns P ≥ 0.05, **P < 0.01.

    Techniques Used: Binding Assay, Luciferase, Transfection, Over Expression, Plasmid Preparation, Western Blot, Expressing, Cotransfection, Standard Deviation

    human bc cell lines mda mb 231  (ATCC)


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    ATCC human bc cell lines mda mb 231
    Human Bc Cell Lines Mda Mb 231, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human bc cell lines mda mb 231/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human bc cell lines mda mb 231 - by Bioz Stars, 2024-04
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    human bc cell line mda mb 231  (ATCC)


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    ATCC human bc cell line mda mb 231
    Polydatin suppressed the combination of PCSK9-LDLR and inhibited the expression of LDL-C in vitro. A. PCSK9 and LDLR protein expression in MCF-10A, CAA, Adi and CAA+polydatin. B. Semi-quantitative analysis of PCSK9 protein. C. Semi-quantitative analysis of LDLR protein. D. PCSK9 mRNA expression. E. LDLR mRNA expression. F. LDL-C protein expression in MCF-10A, CAA, Adi and CAA+polydatin. G. LDL-C mRNA expression. H. Semi-quantitative analysis of LDL-C protein. *P<0.05. MCF-10A, alone cultured human normal mammary epithelial cell line group; CAA, co-culture group; Adi, adipocyte cultured alone group; <t>CAA+polydatin,</t> <t>co-cultured</t> <t>MDA-MB-231</t> and 3T3-L1 cells with polydatin into the co-culture system.
    Human Bc Cell Line Mda Mb 231, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human bc cell line mda mb 231/product/ATCC
    Average 86 stars, based on 1 article reviews
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    1) Product Images from "Polydatin ameliorates low-density lipoprotein cholesterol and lipid metabolism by downregulating proprotein convertase subtilisin/kexin type 9 (PCSK9) in triple-negative breast cancer with hyperlipidemia"

    Article Title: Polydatin ameliorates low-density lipoprotein cholesterol and lipid metabolism by downregulating proprotein convertase subtilisin/kexin type 9 (PCSK9) in triple-negative breast cancer with hyperlipidemia

    Journal: American Journal of Cancer Research

    doi:

    Polydatin suppressed the combination of PCSK9-LDLR and inhibited the expression of LDL-C in vitro. A. PCSK9 and LDLR protein expression in MCF-10A, CAA, Adi and CAA+polydatin. B. Semi-quantitative analysis of PCSK9 protein. C. Semi-quantitative analysis of LDLR protein. D. PCSK9 mRNA expression. E. LDLR mRNA expression. F. LDL-C protein expression in MCF-10A, CAA, Adi and CAA+polydatin. G. LDL-C mRNA expression. H. Semi-quantitative analysis of LDL-C protein. *P<0.05. MCF-10A, alone cultured human normal mammary epithelial cell line group; CAA, co-culture group; Adi, adipocyte cultured alone group; CAA+polydatin, co-cultured MDA-MB-231 and 3T3-L1 cells with polydatin into the co-culture system.
    Figure Legend Snippet: Polydatin suppressed the combination of PCSK9-LDLR and inhibited the expression of LDL-C in vitro. A. PCSK9 and LDLR protein expression in MCF-10A, CAA, Adi and CAA+polydatin. B. Semi-quantitative analysis of PCSK9 protein. C. Semi-quantitative analysis of LDLR protein. D. PCSK9 mRNA expression. E. LDLR mRNA expression. F. LDL-C protein expression in MCF-10A, CAA, Adi and CAA+polydatin. G. LDL-C mRNA expression. H. Semi-quantitative analysis of LDL-C protein. *P<0.05. MCF-10A, alone cultured human normal mammary epithelial cell line group; CAA, co-culture group; Adi, adipocyte cultured alone group; CAA+polydatin, co-cultured MDA-MB-231 and 3T3-L1 cells with polydatin into the co-culture system.

    Techniques Used: Expressing, In Vitro, Cell Culture, Co-Culture Assay

    human bc cell line mda mb 231  (ATCC)


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    ATCC human bc cell line mda mb 231
    Polydatin suppressed the combination of PCSK9-LDLR and inhibited the expression of LDL-C in vitro. A. PCSK9 and LDLR protein expression in MCF-10A, CAA, Adi and CAA+polydatin. B. Semi-quantitative analysis of PCSK9 protein. C. Semi-quantitative analysis of LDLR protein. D. PCSK9 mRNA expression. E. LDLR mRNA expression. F. LDL-C protein expression in MCF-10A, CAA, Adi and CAA+polydatin. G. LDL-C mRNA expression. H. Semi-quantitative analysis of LDL-C protein. *P<0.05. MCF-10A, alone cultured human normal mammary epithelial cell line group; CAA, co-culture group; Adi, adipocyte cultured alone group; <t>CAA+polydatin,</t> <t>co-cultured</t> <t>MDA-MB-231</t> and 3T3-L1 cells with polydatin into the co-culture system.
    Human Bc Cell Line Mda Mb 231, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Polydatin ameliorates low-density lipoprotein cholesterol and lipid metabolism by downregulating proprotein convertase subtilisin/kexin type 9 (PCSK9) in triple-negative breast cancer with hyperlipidemia"

    Article Title: Polydatin ameliorates low-density lipoprotein cholesterol and lipid metabolism by downregulating proprotein convertase subtilisin/kexin type 9 (PCSK9) in triple-negative breast cancer with hyperlipidemia

    Journal: American Journal of Cancer Research

    doi:

    Polydatin suppressed the combination of PCSK9-LDLR and inhibited the expression of LDL-C in vitro. A. PCSK9 and LDLR protein expression in MCF-10A, CAA, Adi and CAA+polydatin. B. Semi-quantitative analysis of PCSK9 protein. C. Semi-quantitative analysis of LDLR protein. D. PCSK9 mRNA expression. E. LDLR mRNA expression. F. LDL-C protein expression in MCF-10A, CAA, Adi and CAA+polydatin. G. LDL-C mRNA expression. H. Semi-quantitative analysis of LDL-C protein. *P<0.05. MCF-10A, alone cultured human normal mammary epithelial cell line group; CAA, co-culture group; Adi, adipocyte cultured alone group; CAA+polydatin, co-cultured MDA-MB-231 and 3T3-L1 cells with polydatin into the co-culture system.
    Figure Legend Snippet: Polydatin suppressed the combination of PCSK9-LDLR and inhibited the expression of LDL-C in vitro. A. PCSK9 and LDLR protein expression in MCF-10A, CAA, Adi and CAA+polydatin. B. Semi-quantitative analysis of PCSK9 protein. C. Semi-quantitative analysis of LDLR protein. D. PCSK9 mRNA expression. E. LDLR mRNA expression. F. LDL-C protein expression in MCF-10A, CAA, Adi and CAA+polydatin. G. LDL-C mRNA expression. H. Semi-quantitative analysis of LDL-C protein. *P<0.05. MCF-10A, alone cultured human normal mammary epithelial cell line group; CAA, co-culture group; Adi, adipocyte cultured alone group; CAA+polydatin, co-cultured MDA-MB-231 and 3T3-L1 cells with polydatin into the co-culture system.

    Techniques Used: Expressing, In Vitro, Cell Culture, Co-Culture Assay

    human triple negative bc cell lines mda mb 231  (ATCC)


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    ATCC human triple negative bc cell lines mda mb 231
    Human Triple Negative Bc Cell Lines Mda Mb 231, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human bc mda mb 231 cell line  (ATCC)


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    ATCC human bc mda mb 231 cell line
    Human Bc Mda Mb 231 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human bc cell lines mda mb 231  (ATCC)


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    ATCC human bc cell lines mda mb 231
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    human bc cell lines mda mb 231  (ATCC)


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    ATCC human bc cell lines mda mb 231
    A , B RT-qPCR analysis of the relative circ_0042881 and NF1 expression after circ_0042881 <t>knockdown</t> <t>in</t> <t>MDA-MB-231</t> and MCF-7. C− F The effect of silence circ_0042881 on proliferation in MDA-MB-231 and MCF-7 was tested by CCK-8, EdU and colony formation assays. G Transwell assays were performed to analyze cell migration and invasion ability. H , I Wound healing assays were used to evaluate the cell migration ability of each group. The data are presented as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001.
    Human Bc Cell Lines Mda Mb 231, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "EIF4A3-mediated circ_0042881 activates the RAS pathway via miR-217/SOS1 axis to facilitate breast cancer progression"

    Article Title: EIF4A3-mediated circ_0042881 activates the RAS pathway via miR-217/SOS1 axis to facilitate breast cancer progression

    Journal: Cell Death & Disease

    doi: 10.1038/s41419-023-06085-4

    A , B RT-qPCR analysis of the relative circ_0042881 and NF1 expression after circ_0042881 knockdown in MDA-MB-231 and MCF-7. C− F The effect of silence circ_0042881 on proliferation in MDA-MB-231 and MCF-7 was tested by CCK-8, EdU and colony formation assays. G Transwell assays were performed to analyze cell migration and invasion ability. H , I Wound healing assays were used to evaluate the cell migration ability of each group. The data are presented as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001.
    Figure Legend Snippet: A , B RT-qPCR analysis of the relative circ_0042881 and NF1 expression after circ_0042881 knockdown in MDA-MB-231 and MCF-7. C− F The effect of silence circ_0042881 on proliferation in MDA-MB-231 and MCF-7 was tested by CCK-8, EdU and colony formation assays. G Transwell assays were performed to analyze cell migration and invasion ability. H , I Wound healing assays were used to evaluate the cell migration ability of each group. The data are presented as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001.

    Techniques Used: Quantitative RT-PCR, Expressing, CCK-8 Assay, Migration

    A , B RIP experiments were performed, and RT‐qPCR assays and agarose gel electrophoresis were used to detect the enrichment of circ_0042881 to AGO2. C Venn diagram representing the potential targeted miRNAs of circ_0042881 by CircInteractome, circBANK, Starbase v3.0. D RT-qPCR analyzed the relative expression of 4 candidate miRNAs in MDA-MB-231 and MCF-7 after transfecting with si-circ_0042881. E The relative expression of miR-217 and miR-338-5p was tested by RT-qPCR after circ_0042881 overexpression. F To examine the direct interaction between circ_0042881 and miR-217, the luciferase reporter vectors containing circ_0042881 WT or circ_0042881 MUT were constructed. G The correlation between circ_0042881 and miR-217 in BC tissues was analyzed by Pearson correlation analysis. H The luciferase activities in MDA-MB-231 and MCF-7 cells co-transfected with miR-217 mimic or mimic NC and luciferase reporters containing circ_0042881 WT or circ_0042881 MUT. The data are presented as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001.
    Figure Legend Snippet: A , B RIP experiments were performed, and RT‐qPCR assays and agarose gel electrophoresis were used to detect the enrichment of circ_0042881 to AGO2. C Venn diagram representing the potential targeted miRNAs of circ_0042881 by CircInteractome, circBANK, Starbase v3.0. D RT-qPCR analyzed the relative expression of 4 candidate miRNAs in MDA-MB-231 and MCF-7 after transfecting with si-circ_0042881. E The relative expression of miR-217 and miR-338-5p was tested by RT-qPCR after circ_0042881 overexpression. F To examine the direct interaction between circ_0042881 and miR-217, the luciferase reporter vectors containing circ_0042881 WT or circ_0042881 MUT were constructed. G The correlation between circ_0042881 and miR-217 in BC tissues was analyzed by Pearson correlation analysis. H The luciferase activities in MDA-MB-231 and MCF-7 cells co-transfected with miR-217 mimic or mimic NC and luciferase reporters containing circ_0042881 WT or circ_0042881 MUT. The data are presented as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001.

    Techniques Used: Quantitative RT-PCR, Agarose Gel Electrophoresis, Expressing, Over Expression, Luciferase, Construct, Transfection

    A Volcano plot showing differentially expressed genes in MDA-MB-231 cells transfected with mimic NC and miR-217 mimic. B Venn diagram representing the potential targeted mRNAs of miR-217 by miRDB, TargetScan, Starbase v3.0 and RNA-seq results. C , D RT-qPCR analyzed the relative expression of 5 candidate mRNAs in MDA-MB-231 and MCF-7 after transfecting with si-circ_0042881. E , F The luciferase activities in MDA-MB-231 and MCF-7 cells co-transfected with miR-217 mimic or mimic NC and luciferase reporters containing SOS1 3’UTR WT or SOS1 3’UTR MUT. G The correlation between circ_0042881 and SOS1 in BC tissues was analyzed by Pearson correlation analysis. H , I Western blotting analyed the protein levels of SOS1, AKT, p-AKT, ERK, and p-ERK after corresponding treatment. The data are presented as mean ± SD. ** P < 0.01, *** P < 0.001.
    Figure Legend Snippet: A Volcano plot showing differentially expressed genes in MDA-MB-231 cells transfected with mimic NC and miR-217 mimic. B Venn diagram representing the potential targeted mRNAs of miR-217 by miRDB, TargetScan, Starbase v3.0 and RNA-seq results. C , D RT-qPCR analyzed the relative expression of 5 candidate mRNAs in MDA-MB-231 and MCF-7 after transfecting with si-circ_0042881. E , F The luciferase activities in MDA-MB-231 and MCF-7 cells co-transfected with miR-217 mimic or mimic NC and luciferase reporters containing SOS1 3’UTR WT or SOS1 3’UTR MUT. G The correlation between circ_0042881 and SOS1 in BC tissues was analyzed by Pearson correlation analysis. H , I Western blotting analyed the protein levels of SOS1, AKT, p-AKT, ERK, and p-ERK after corresponding treatment. The data are presented as mean ± SD. ** P < 0.01, *** P < 0.001.

    Techniques Used: Transfection, RNA Sequencing Assay, Quantitative RT-PCR, Expressing, Luciferase, Western Blot

    A The binding sites for EIF4A3 in the flanking sequences of the NF1 pre-mRNA transcript were predicted using CircInteractome and RIP assays validated the binding sites between NF1 and circ_0042881. B The relative expression of EIF4A3 and circ_0042881 after EIF4A3 knockdown was detected by RT-qPCR. C The protein level of EIF4A3 after EIF4A3 knockdown was detected by western blotting. D The correlation between circ_0042881 and EIF4A3 in BC tissues was analyzed by Pearson correlation analysis. E Representative images of immunohistostaining for EIF4A3 of tumor and adjacent non-tumor tissues ( n = 30). F − H The proliferation of MDA-MB-231 was detected by CCK-8 and EdU assays. I , J Representative results of transwell and wound healing assays of each group.
    Figure Legend Snippet: A The binding sites for EIF4A3 in the flanking sequences of the NF1 pre-mRNA transcript were predicted using CircInteractome and RIP assays validated the binding sites between NF1 and circ_0042881. B The relative expression of EIF4A3 and circ_0042881 after EIF4A3 knockdown was detected by RT-qPCR. C The protein level of EIF4A3 after EIF4A3 knockdown was detected by western blotting. D The correlation between circ_0042881 and EIF4A3 in BC tissues was analyzed by Pearson correlation analysis. E Representative images of immunohistostaining for EIF4A3 of tumor and adjacent non-tumor tissues ( n = 30). F − H The proliferation of MDA-MB-231 was detected by CCK-8 and EdU assays. I , J Representative results of transwell and wound healing assays of each group.

    Techniques Used: Binding Assay, Expressing, Quantitative RT-PCR, Western Blot, CCK-8 Assay

    human mda mb 231 bc cell line  (ATCC)


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    ATCC human mda mb 231 bc cell line
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    ATCC human bc cell lines mda mb 231
    (A) Flow chart illustrating the steps for miRNA expression microarray analysis using AffymetrixGeneChip ® . (B) Heat maps with hierarchical clustering showing the differential expression of miRNAs in <t>MM231</t> wild-type , MM231 resistant-type , MM468 wild-type , MM468 resistant-type , HCC1395 wild-type , and HCC1395 resistant-type BC cell lines. (C) Venn diagrams illustrating the number of all upregulated and downregulated DEMs in three pairs of BC cell lines. The intersection in the center represents the common DEMs among the three groups. miRNA or miR, microRNA; BC, breast cancer; DEMs, differentially expressed miRNAs.
    Human Bc Cell Lines Mda Mb 231, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC human bc cell line mda mb 231
    Polydatin suppressed the combination of PCSK9-LDLR and inhibited the expression of LDL-C in vitro. A. PCSK9 and LDLR protein expression in MCF-10A, CAA, Adi and CAA+polydatin. B. Semi-quantitative analysis of PCSK9 protein. C. Semi-quantitative analysis of LDLR protein. D. PCSK9 mRNA expression. E. LDLR mRNA expression. F. LDL-C protein expression in MCF-10A, CAA, Adi and CAA+polydatin. G. LDL-C mRNA expression. H. Semi-quantitative analysis of LDL-C protein. *P<0.05. MCF-10A, alone cultured human normal mammary epithelial cell line group; CAA, co-culture group; Adi, adipocyte cultured alone group; <t>CAA+polydatin,</t> <t>co-cultured</t> <t>MDA-MB-231</t> and 3T3-L1 cells with polydatin into the co-culture system.
    Human Bc Cell Line Mda Mb 231, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC human triple negative bc cell lines mda mb 231
    Polydatin suppressed the combination of PCSK9-LDLR and inhibited the expression of LDL-C in vitro. A. PCSK9 and LDLR protein expression in MCF-10A, CAA, Adi and CAA+polydatin. B. Semi-quantitative analysis of PCSK9 protein. C. Semi-quantitative analysis of LDLR protein. D. PCSK9 mRNA expression. E. LDLR mRNA expression. F. LDL-C protein expression in MCF-10A, CAA, Adi and CAA+polydatin. G. LDL-C mRNA expression. H. Semi-quantitative analysis of LDL-C protein. *P<0.05. MCF-10A, alone cultured human normal mammary epithelial cell line group; CAA, co-culture group; Adi, adipocyte cultured alone group; <t>CAA+polydatin,</t> <t>co-cultured</t> <t>MDA-MB-231</t> and 3T3-L1 cells with polydatin into the co-culture system.
    Human Triple Negative Bc Cell Lines Mda Mb 231, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC human bc mda mb 231 cell line
    Polydatin suppressed the combination of PCSK9-LDLR and inhibited the expression of LDL-C in vitro. A. PCSK9 and LDLR protein expression in MCF-10A, CAA, Adi and CAA+polydatin. B. Semi-quantitative analysis of PCSK9 protein. C. Semi-quantitative analysis of LDLR protein. D. PCSK9 mRNA expression. E. LDLR mRNA expression. F. LDL-C protein expression in MCF-10A, CAA, Adi and CAA+polydatin. G. LDL-C mRNA expression. H. Semi-quantitative analysis of LDL-C protein. *P<0.05. MCF-10A, alone cultured human normal mammary epithelial cell line group; CAA, co-culture group; Adi, adipocyte cultured alone group; <t>CAA+polydatin,</t> <t>co-cultured</t> <t>MDA-MB-231</t> and 3T3-L1 cells with polydatin into the co-culture system.
    Human Bc Mda Mb 231 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC human mda mb 231 bc cell line
    Polydatin suppressed the combination of PCSK9-LDLR and inhibited the expression of LDL-C in vitro. A. PCSK9 and LDLR protein expression in MCF-10A, CAA, Adi and CAA+polydatin. B. Semi-quantitative analysis of PCSK9 protein. C. Semi-quantitative analysis of LDLR protein. D. PCSK9 mRNA expression. E. LDLR mRNA expression. F. LDL-C protein expression in MCF-10A, CAA, Adi and CAA+polydatin. G. LDL-C mRNA expression. H. Semi-quantitative analysis of LDL-C protein. *P<0.05. MCF-10A, alone cultured human normal mammary epithelial cell line group; CAA, co-culture group; Adi, adipocyte cultured alone group; <t>CAA+polydatin,</t> <t>co-cultured</t> <t>MDA-MB-231</t> and 3T3-L1 cells with polydatin into the co-culture system.
    Human Mda Mb 231 Bc Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (A) Flow chart illustrating the steps for miRNA expression microarray analysis using AffymetrixGeneChip ® . (B) Heat maps with hierarchical clustering showing the differential expression of miRNAs in MM231 wild-type , MM231 resistant-type , MM468 wild-type , MM468 resistant-type , HCC1395 wild-type , and HCC1395 resistant-type BC cell lines. (C) Venn diagrams illustrating the number of all upregulated and downregulated DEMs in three pairs of BC cell lines. The intersection in the center represents the common DEMs among the three groups. miRNA or miR, microRNA; BC, breast cancer; DEMs, differentially expressed miRNAs.

    Journal: Oncology Letters

    Article Title: Exploring miRNA‑target gene profiles associated with drug resistance in patients with breast cancer receiving neoadjuvant chemotherapy

    doi: 10.3892/ol.2024.14291

    Figure Lengend Snippet: (A) Flow chart illustrating the steps for miRNA expression microarray analysis using AffymetrixGeneChip ® . (B) Heat maps with hierarchical clustering showing the differential expression of miRNAs in MM231 wild-type , MM231 resistant-type , MM468 wild-type , MM468 resistant-type , HCC1395 wild-type , and HCC1395 resistant-type BC cell lines. (C) Venn diagrams illustrating the number of all upregulated and downregulated DEMs in three pairs of BC cell lines. The intersection in the center represents the common DEMs among the three groups. miRNA or miR, microRNA; BC, breast cancer; DEMs, differentially expressed miRNAs.

    Article Snippet: Human BC cell lines MDA-MB-231 (MM231; HTB-26), MDA-MB-468 (MM468; HTB-132), and HCC1395 (CRL-2324) were purchased from the American Type Culture Collection.

    Techniques: Expressing, Microarray

    (A) Venn diagram showing candidate exosomal miRNAs related to drug resistance. Bar charts showing fold-change in exosomal miRNAs from wild-type and resistant-type cells. A total of 12 selected candidate exosomal miRNAs were analyzed in the three different breast cancer cell lines (B) MM231, (C) MM468, and (D) HCC1395, using quantitative PCR. Significant miRNAs are indicated in bold text. miRNA or miR, microRNA.

    Journal: Oncology Letters

    Article Title: Exploring miRNA‑target gene profiles associated with drug resistance in patients with breast cancer receiving neoadjuvant chemotherapy

    doi: 10.3892/ol.2024.14291

    Figure Lengend Snippet: (A) Venn diagram showing candidate exosomal miRNAs related to drug resistance. Bar charts showing fold-change in exosomal miRNAs from wild-type and resistant-type cells. A total of 12 selected candidate exosomal miRNAs were analyzed in the three different breast cancer cell lines (B) MM231, (C) MM468, and (D) HCC1395, using quantitative PCR. Significant miRNAs are indicated in bold text. miRNA or miR, microRNA.

    Article Snippet: Human BC cell lines MDA-MB-231 (MM231; HTB-26), MDA-MB-468 (MM468; HTB-132), and HCC1395 (CRL-2324) were purchased from the American Type Culture Collection.

    Techniques: Real-time Polymerase Chain Reaction

    (A) Radar plots of miRNA signature profiles from quantitative PCR in BC cells, including MDA-MB-231 (MM231), MDA-MB-468 (MM468), and HCC1395. The relative expression levels of miRNAs in wild-type BC cells, drug-resistant exosome 24 h-treated (educated-type) BC cells, and resistant-type BC cells were compared. The line labels represent the drug-resistant miRNAs, and the ring labels represent the fold change calculated from each group/wild-type difference. (B) The cell viabilities of wild-type, educated-type, and resistant-type BC cells were compared after 48 h of incubation with Adriamycin and Taxotere. miRNA or miR, microRNA; BC, breast cancer; ADR, Adriamycin; TAX, Taxotere.

    Journal: Oncology Letters

    Article Title: Exploring miRNA‑target gene profiles associated with drug resistance in patients with breast cancer receiving neoadjuvant chemotherapy

    doi: 10.3892/ol.2024.14291

    Figure Lengend Snippet: (A) Radar plots of miRNA signature profiles from quantitative PCR in BC cells, including MDA-MB-231 (MM231), MDA-MB-468 (MM468), and HCC1395. The relative expression levels of miRNAs in wild-type BC cells, drug-resistant exosome 24 h-treated (educated-type) BC cells, and resistant-type BC cells were compared. The line labels represent the drug-resistant miRNAs, and the ring labels represent the fold change calculated from each group/wild-type difference. (B) The cell viabilities of wild-type, educated-type, and resistant-type BC cells were compared after 48 h of incubation with Adriamycin and Taxotere. miRNA or miR, microRNA; BC, breast cancer; ADR, Adriamycin; TAX, Taxotere.

    Article Snippet: Human BC cell lines MDA-MB-231 (MM231; HTB-26), MDA-MB-468 (MM468; HTB-132), and HCC1395 (CRL-2324) were purchased from the American Type Culture Collection.

    Techniques: Real-time Polymerase Chain Reaction, Expressing, Incubation

    Polydatin suppressed the combination of PCSK9-LDLR and inhibited the expression of LDL-C in vitro. A. PCSK9 and LDLR protein expression in MCF-10A, CAA, Adi and CAA+polydatin. B. Semi-quantitative analysis of PCSK9 protein. C. Semi-quantitative analysis of LDLR protein. D. PCSK9 mRNA expression. E. LDLR mRNA expression. F. LDL-C protein expression in MCF-10A, CAA, Adi and CAA+polydatin. G. LDL-C mRNA expression. H. Semi-quantitative analysis of LDL-C protein. *P<0.05. MCF-10A, alone cultured human normal mammary epithelial cell line group; CAA, co-culture group; Adi, adipocyte cultured alone group; CAA+polydatin, co-cultured MDA-MB-231 and 3T3-L1 cells with polydatin into the co-culture system.

    Journal: American Journal of Cancer Research

    Article Title: Polydatin ameliorates low-density lipoprotein cholesterol and lipid metabolism by downregulating proprotein convertase subtilisin/kexin type 9 (PCSK9) in triple-negative breast cancer with hyperlipidemia

    doi:

    Figure Lengend Snippet: Polydatin suppressed the combination of PCSK9-LDLR and inhibited the expression of LDL-C in vitro. A. PCSK9 and LDLR protein expression in MCF-10A, CAA, Adi and CAA+polydatin. B. Semi-quantitative analysis of PCSK9 protein. C. Semi-quantitative analysis of LDLR protein. D. PCSK9 mRNA expression. E. LDLR mRNA expression. F. LDL-C protein expression in MCF-10A, CAA, Adi and CAA+polydatin. G. LDL-C mRNA expression. H. Semi-quantitative analysis of LDL-C protein. *P<0.05. MCF-10A, alone cultured human normal mammary epithelial cell line group; CAA, co-culture group; Adi, adipocyte cultured alone group; CAA+polydatin, co-cultured MDA-MB-231 and 3T3-L1 cells with polydatin into the co-culture system.

    Article Snippet: Cell lines and culture conditions The American Type Culture Collection (ATCC, Rockville, MD, USA) provided the human BC cell line MDA-MB-231 and the mouse mammary cancer cell line 4T1-Luc.

    Techniques: Expressing, In Vitro, Cell Culture, Co-Culture Assay