human adult lung fibroblast line hs888lu  (ATCC)


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    ATCC human adult lung fibroblast line hs888lu
    MUC1 is expressed and contributes to EREG expression in fibroblasts. (A) Normal lung tissues from seven control WT and seven Muc1 KO mice and tumor tissues from seven NNK-treated WT and seven Muc1 KO mice were used to determine EREG concentration by ELISA assay. Ten micrograms of total protein from each sample was loaded to each well. Data shown are mean ± S.D; **P < 0.01. (B) Left, HFL-1 cells were treated with CSE (0, 10 and 20 µg/ml TPM) overnight. MUC1 expression was detected by Western blot. β-Actin was detected as an input control. Middle, HFL-1 cells were treated with CSE (20 µg/ml TPM) for the indicated time points. MUC1 mRNA level was detected by RT-PCR. β-Actin was detected as an input control. Right, HFL-1 cells were transfected with MUC1 siRNA or negative control siRNA for 48 h. MUC1 expression was detected by Western blot with antibody Muc1 GP1.4 against the extracellular domain (MUC1-N) and antibody MUC1 Ab-5 recognizing the C-terminal domain (MUC1-CT). β-Actin was detected as an input control. (C) HFL-1 and <t>Hs888Lu</t> cells were transfected with MUC1 siRNA or negative control siRNA for 24 h, before the cells were treated with CSE (20 µg/ml TPM), TNF-α (10 ng/ml), IL-6 (10 ng/ml) and IL-1β (10 ng/ml) overnight. EREG expression was detected by Western blot. β-Actin was detected as an input control. The intensity of the individual bands was quantified and normalized to the corresponding input control bands. Fold changes were calculated with the control taken as 1.
    Human Adult Lung Fibroblast Line Hs888lu, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human adult lung fibroblast line hs888lu/product/ATCC
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human adult lung fibroblast line hs888lu - by Bioz Stars, 2024-05
    92/100 stars

    Images

    1) Product Images from "Muc1 knockout potentiates murine lung carcinogenesis involving an epiregulin-mediated EGFR activation feedback loop"

    Article Title: Muc1 knockout potentiates murine lung carcinogenesis involving an epiregulin-mediated EGFR activation feedback loop

    Journal: Carcinogenesis

    doi: 10.1093/carcin/bgx039

    MUC1 is expressed and contributes to EREG expression in fibroblasts. (A) Normal lung tissues from seven control WT and seven Muc1 KO mice and tumor tissues from seven NNK-treated WT and seven Muc1 KO mice were used to determine EREG concentration by ELISA assay. Ten micrograms of total protein from each sample was loaded to each well. Data shown are mean ± S.D; **P < 0.01. (B) Left, HFL-1 cells were treated with CSE (0, 10 and 20 µg/ml TPM) overnight. MUC1 expression was detected by Western blot. β-Actin was detected as an input control. Middle, HFL-1 cells were treated with CSE (20 µg/ml TPM) for the indicated time points. MUC1 mRNA level was detected by RT-PCR. β-Actin was detected as an input control. Right, HFL-1 cells were transfected with MUC1 siRNA or negative control siRNA for 48 h. MUC1 expression was detected by Western blot with antibody Muc1 GP1.4 against the extracellular domain (MUC1-N) and antibody MUC1 Ab-5 recognizing the C-terminal domain (MUC1-CT). β-Actin was detected as an input control. (C) HFL-1 and Hs888Lu cells were transfected with MUC1 siRNA or negative control siRNA for 24 h, before the cells were treated with CSE (20 µg/ml TPM), TNF-α (10 ng/ml), IL-6 (10 ng/ml) and IL-1β (10 ng/ml) overnight. EREG expression was detected by Western blot. β-Actin was detected as an input control. The intensity of the individual bands was quantified and normalized to the corresponding input control bands. Fold changes were calculated with the control taken as 1.
    Figure Legend Snippet: MUC1 is expressed and contributes to EREG expression in fibroblasts. (A) Normal lung tissues from seven control WT and seven Muc1 KO mice and tumor tissues from seven NNK-treated WT and seven Muc1 KO mice were used to determine EREG concentration by ELISA assay. Ten micrograms of total protein from each sample was loaded to each well. Data shown are mean ± S.D; **P < 0.01. (B) Left, HFL-1 cells were treated with CSE (0, 10 and 20 µg/ml TPM) overnight. MUC1 expression was detected by Western blot. β-Actin was detected as an input control. Middle, HFL-1 cells were treated with CSE (20 µg/ml TPM) for the indicated time points. MUC1 mRNA level was detected by RT-PCR. β-Actin was detected as an input control. Right, HFL-1 cells were transfected with MUC1 siRNA or negative control siRNA for 48 h. MUC1 expression was detected by Western blot with antibody Muc1 GP1.4 against the extracellular domain (MUC1-N) and antibody MUC1 Ab-5 recognizing the C-terminal domain (MUC1-CT). β-Actin was detected as an input control. (C) HFL-1 and Hs888Lu cells were transfected with MUC1 siRNA or negative control siRNA for 24 h, before the cells were treated with CSE (20 µg/ml TPM), TNF-α (10 ng/ml), IL-6 (10 ng/ml) and IL-1β (10 ng/ml) overnight. EREG expression was detected by Western blot. β-Actin was detected as an input control. The intensity of the individual bands was quantified and normalized to the corresponding input control bands. Fold changes were calculated with the control taken as 1.

    Techniques Used: Expressing, Concentration Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Reverse Transcription Polymerase Chain Reaction, Transfection, Negative Control

    human adult lung fibroblast line hs888lu  (ATCC)


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    Bioz Manufacturer Symbol ATCC manufactures this product  
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  • 92

    Structured Review

    ATCC human adult lung fibroblast line hs888lu
    MUC1 is expressed and contributes to EREG expression in fibroblasts. (A) Normal lung tissues from seven control WT and seven Muc1 KO mice and tumor tissues from seven NNK-treated WT and seven Muc1 KO mice were used to determine EREG concentration by ELISA assay. Ten micrograms of total protein from each sample was loaded to each well. Data shown are mean ± S.D; **P < 0.01. (B) Left, HFL-1 cells were treated with CSE (0, 10 and 20 µg/ml TPM) overnight. MUC1 expression was detected by Western blot. β-Actin was detected as an input control. Middle, HFL-1 cells were treated with CSE (20 µg/ml TPM) for the indicated time points. MUC1 mRNA level was detected by RT-PCR. β-Actin was detected as an input control. Right, HFL-1 cells were transfected with MUC1 siRNA or negative control siRNA for 48 h. MUC1 expression was detected by Western blot with antibody Muc1 GP1.4 against the extracellular domain (MUC1-N) and antibody MUC1 Ab-5 recognizing the C-terminal domain (MUC1-CT). β-Actin was detected as an input control. (C) HFL-1 and <t>Hs888Lu</t> cells were transfected with MUC1 siRNA or negative control siRNA for 24 h, before the cells were treated with CSE (20 µg/ml TPM), TNF-α (10 ng/ml), IL-6 (10 ng/ml) and IL-1β (10 ng/ml) overnight. EREG expression was detected by Western blot. β-Actin was detected as an input control. The intensity of the individual bands was quantified and normalized to the corresponding input control bands. Fold changes were calculated with the control taken as 1.
    Human Adult Lung Fibroblast Line Hs888lu, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human adult lung fibroblast line hs888lu/product/ATCC
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human adult lung fibroblast line hs888lu - by Bioz Stars, 2024-05
    92/100 stars

    Images

    1) Product Images from "Muc1 knockout potentiates murine lung carcinogenesis involving an epiregulin-mediated EGFR activation feedback loop"

    Article Title: Muc1 knockout potentiates murine lung carcinogenesis involving an epiregulin-mediated EGFR activation feedback loop

    Journal: Carcinogenesis

    doi: 10.1093/carcin/bgx039

    MUC1 is expressed and contributes to EREG expression in fibroblasts. (A) Normal lung tissues from seven control WT and seven Muc1 KO mice and tumor tissues from seven NNK-treated WT and seven Muc1 KO mice were used to determine EREG concentration by ELISA assay. Ten micrograms of total protein from each sample was loaded to each well. Data shown are mean ± S.D; **P < 0.01. (B) Left, HFL-1 cells were treated with CSE (0, 10 and 20 µg/ml TPM) overnight. MUC1 expression was detected by Western blot. β-Actin was detected as an input control. Middle, HFL-1 cells were treated with CSE (20 µg/ml TPM) for the indicated time points. MUC1 mRNA level was detected by RT-PCR. β-Actin was detected as an input control. Right, HFL-1 cells were transfected with MUC1 siRNA or negative control siRNA for 48 h. MUC1 expression was detected by Western blot with antibody Muc1 GP1.4 against the extracellular domain (MUC1-N) and antibody MUC1 Ab-5 recognizing the C-terminal domain (MUC1-CT). β-Actin was detected as an input control. (C) HFL-1 and Hs888Lu cells were transfected with MUC1 siRNA or negative control siRNA for 24 h, before the cells were treated with CSE (20 µg/ml TPM), TNF-α (10 ng/ml), IL-6 (10 ng/ml) and IL-1β (10 ng/ml) overnight. EREG expression was detected by Western blot. β-Actin was detected as an input control. The intensity of the individual bands was quantified and normalized to the corresponding input control bands. Fold changes were calculated with the control taken as 1.
    Figure Legend Snippet: MUC1 is expressed and contributes to EREG expression in fibroblasts. (A) Normal lung tissues from seven control WT and seven Muc1 KO mice and tumor tissues from seven NNK-treated WT and seven Muc1 KO mice were used to determine EREG concentration by ELISA assay. Ten micrograms of total protein from each sample was loaded to each well. Data shown are mean ± S.D; **P < 0.01. (B) Left, HFL-1 cells were treated with CSE (0, 10 and 20 µg/ml TPM) overnight. MUC1 expression was detected by Western blot. β-Actin was detected as an input control. Middle, HFL-1 cells were treated with CSE (20 µg/ml TPM) for the indicated time points. MUC1 mRNA level was detected by RT-PCR. β-Actin was detected as an input control. Right, HFL-1 cells were transfected with MUC1 siRNA or negative control siRNA for 48 h. MUC1 expression was detected by Western blot with antibody Muc1 GP1.4 against the extracellular domain (MUC1-N) and antibody MUC1 Ab-5 recognizing the C-terminal domain (MUC1-CT). β-Actin was detected as an input control. (C) HFL-1 and Hs888Lu cells were transfected with MUC1 siRNA or negative control siRNA for 24 h, before the cells were treated with CSE (20 µg/ml TPM), TNF-α (10 ng/ml), IL-6 (10 ng/ml) and IL-1β (10 ng/ml) overnight. EREG expression was detected by Western blot. β-Actin was detected as an input control. The intensity of the individual bands was quantified and normalized to the corresponding input control bands. Fold changes were calculated with the control taken as 1.

    Techniques Used: Expressing, Concentration Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Reverse Transcription Polymerase Chain Reaction, Transfection, Negative Control

    human adult lung fibroblast line hs888lu  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
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  • 92

    Structured Review

    ATCC human adult lung fibroblast line hs888lu
    MUC1 is expressed and contributes to EREG expression in fibroblasts. (A) Normal lung tissues from seven control WT and seven Muc1 KO mice and tumor tissues from seven NNK-treated WT and seven Muc1 KO mice were used to determine EREG concentration by ELISA assay. Ten micrograms of total protein from each sample was loaded to each well. Data shown are mean ± S.D; **P < 0.01. (B) Left, HFL-1 cells were treated with CSE (0, 10 and 20 µg/ml TPM) overnight. MUC1 expression was detected by Western blot. β-Actin was detected as an input control. Middle, HFL-1 cells were treated with CSE (20 µg/ml TPM) for the indicated time points. MUC1 mRNA level was detected by RT-PCR. β-Actin was detected as an input control. Right, HFL-1 cells were transfected with MUC1 siRNA or negative control siRNA for 48 h. MUC1 expression was detected by Western blot with antibody Muc1 GP1.4 against the extracellular domain (MUC1-N) and antibody MUC1 Ab-5 recognizing the C-terminal domain (MUC1-CT). β-Actin was detected as an input control. (C) HFL-1 and <t>Hs888Lu</t> cells were transfected with MUC1 siRNA or negative control siRNA for 24 h, before the cells were treated with CSE (20 µg/ml TPM), TNF-α (10 ng/ml), IL-6 (10 ng/ml) and IL-1β (10 ng/ml) overnight. EREG expression was detected by Western blot. β-Actin was detected as an input control. The intensity of the individual bands was quantified and normalized to the corresponding input control bands. Fold changes were calculated with the control taken as 1.
    Human Adult Lung Fibroblast Line Hs888lu, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human adult lung fibroblast line hs888lu/product/ATCC
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human adult lung fibroblast line hs888lu - by Bioz Stars, 2024-05
    92/100 stars

    Images

    1) Product Images from "Muc1 knockout potentiates murine lung carcinogenesis involving an epiregulin-mediated EGFR activation feedback loop"

    Article Title: Muc1 knockout potentiates murine lung carcinogenesis involving an epiregulin-mediated EGFR activation feedback loop

    Journal: Carcinogenesis

    doi: 10.1093/carcin/bgx039

    MUC1 is expressed and contributes to EREG expression in fibroblasts. (A) Normal lung tissues from seven control WT and seven Muc1 KO mice and tumor tissues from seven NNK-treated WT and seven Muc1 KO mice were used to determine EREG concentration by ELISA assay. Ten micrograms of total protein from each sample was loaded to each well. Data shown are mean ± S.D; **P < 0.01. (B) Left, HFL-1 cells were treated with CSE (0, 10 and 20 µg/ml TPM) overnight. MUC1 expression was detected by Western blot. β-Actin was detected as an input control. Middle, HFL-1 cells were treated with CSE (20 µg/ml TPM) for the indicated time points. MUC1 mRNA level was detected by RT-PCR. β-Actin was detected as an input control. Right, HFL-1 cells were transfected with MUC1 siRNA or negative control siRNA for 48 h. MUC1 expression was detected by Western blot with antibody Muc1 GP1.4 against the extracellular domain (MUC1-N) and antibody MUC1 Ab-5 recognizing the C-terminal domain (MUC1-CT). β-Actin was detected as an input control. (C) HFL-1 and Hs888Lu cells were transfected with MUC1 siRNA or negative control siRNA for 24 h, before the cells were treated with CSE (20 µg/ml TPM), TNF-α (10 ng/ml), IL-6 (10 ng/ml) and IL-1β (10 ng/ml) overnight. EREG expression was detected by Western blot. β-Actin was detected as an input control. The intensity of the individual bands was quantified and normalized to the corresponding input control bands. Fold changes were calculated with the control taken as 1.
    Figure Legend Snippet: MUC1 is expressed and contributes to EREG expression in fibroblasts. (A) Normal lung tissues from seven control WT and seven Muc1 KO mice and tumor tissues from seven NNK-treated WT and seven Muc1 KO mice were used to determine EREG concentration by ELISA assay. Ten micrograms of total protein from each sample was loaded to each well. Data shown are mean ± S.D; **P < 0.01. (B) Left, HFL-1 cells were treated with CSE (0, 10 and 20 µg/ml TPM) overnight. MUC1 expression was detected by Western blot. β-Actin was detected as an input control. Middle, HFL-1 cells were treated with CSE (20 µg/ml TPM) for the indicated time points. MUC1 mRNA level was detected by RT-PCR. β-Actin was detected as an input control. Right, HFL-1 cells were transfected with MUC1 siRNA or negative control siRNA for 48 h. MUC1 expression was detected by Western blot with antibody Muc1 GP1.4 against the extracellular domain (MUC1-N) and antibody MUC1 Ab-5 recognizing the C-terminal domain (MUC1-CT). β-Actin was detected as an input control. (C) HFL-1 and Hs888Lu cells were transfected with MUC1 siRNA or negative control siRNA for 24 h, before the cells were treated with CSE (20 µg/ml TPM), TNF-α (10 ng/ml), IL-6 (10 ng/ml) and IL-1β (10 ng/ml) overnight. EREG expression was detected by Western blot. β-Actin was detected as an input control. The intensity of the individual bands was quantified and normalized to the corresponding input control bands. Fold changes were calculated with the control taken as 1.

    Techniques Used: Expressing, Concentration Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Reverse Transcription Polymerase Chain Reaction, Transfection, Negative Control

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    ATCC human adult lung fibroblast line hs888lu
    MUC1 is expressed and contributes to EREG expression in fibroblasts. (A) Normal lung tissues from seven control WT and seven Muc1 KO mice and tumor tissues from seven NNK-treated WT and seven Muc1 KO mice were used to determine EREG concentration by ELISA assay. Ten micrograms of total protein from each sample was loaded to each well. Data shown are mean ± S.D; **P < 0.01. (B) Left, HFL-1 cells were treated with CSE (0, 10 and 20 µg/ml TPM) overnight. MUC1 expression was detected by Western blot. β-Actin was detected as an input control. Middle, HFL-1 cells were treated with CSE (20 µg/ml TPM) for the indicated time points. MUC1 mRNA level was detected by RT-PCR. β-Actin was detected as an input control. Right, HFL-1 cells were transfected with MUC1 siRNA or negative control siRNA for 48 h. MUC1 expression was detected by Western blot with antibody Muc1 GP1.4 against the extracellular domain (MUC1-N) and antibody MUC1 Ab-5 recognizing the C-terminal domain (MUC1-CT). β-Actin was detected as an input control. (C) HFL-1 and <t>Hs888Lu</t> cells were transfected with MUC1 siRNA or negative control siRNA for 24 h, before the cells were treated with CSE (20 µg/ml TPM), TNF-α (10 ng/ml), IL-6 (10 ng/ml) and IL-1β (10 ng/ml) overnight. EREG expression was detected by Western blot. β-Actin was detected as an input control. The intensity of the individual bands was quantified and normalized to the corresponding input control bands. Fold changes were calculated with the control taken as 1.
    Human Adult Lung Fibroblast Line Hs888lu, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human adult lung fibroblast line hs888lu/product/ATCC
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human adult lung fibroblast line hs888lu - by Bioz Stars, 2024-05
    92/100 stars
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    MUC1 is expressed and contributes to EREG expression in fibroblasts. (A) Normal lung tissues from seven control WT and seven Muc1 KO mice and tumor tissues from seven NNK-treated WT and seven Muc1 KO mice were used to determine EREG concentration by ELISA assay. Ten micrograms of total protein from each sample was loaded to each well. Data shown are mean ± S.D; **P < 0.01. (B) Left, HFL-1 cells were treated with CSE (0, 10 and 20 µg/ml TPM) overnight. MUC1 expression was detected by Western blot. β-Actin was detected as an input control. Middle, HFL-1 cells were treated with CSE (20 µg/ml TPM) for the indicated time points. MUC1 mRNA level was detected by RT-PCR. β-Actin was detected as an input control. Right, HFL-1 cells were transfected with MUC1 siRNA or negative control siRNA for 48 h. MUC1 expression was detected by Western blot with antibody Muc1 GP1.4 against the extracellular domain (MUC1-N) and antibody MUC1 Ab-5 recognizing the C-terminal domain (MUC1-CT). β-Actin was detected as an input control. (C) HFL-1 and Hs888Lu cells were transfected with MUC1 siRNA or negative control siRNA for 24 h, before the cells were treated with CSE (20 µg/ml TPM), TNF-α (10 ng/ml), IL-6 (10 ng/ml) and IL-1β (10 ng/ml) overnight. EREG expression was detected by Western blot. β-Actin was detected as an input control. The intensity of the individual bands was quantified and normalized to the corresponding input control bands. Fold changes were calculated with the control taken as 1.

    Journal: Carcinogenesis

    Article Title: Muc1 knockout potentiates murine lung carcinogenesis involving an epiregulin-mediated EGFR activation feedback loop

    doi: 10.1093/carcin/bgx039

    Figure Lengend Snippet: MUC1 is expressed and contributes to EREG expression in fibroblasts. (A) Normal lung tissues from seven control WT and seven Muc1 KO mice and tumor tissues from seven NNK-treated WT and seven Muc1 KO mice were used to determine EREG concentration by ELISA assay. Ten micrograms of total protein from each sample was loaded to each well. Data shown are mean ± S.D; **P < 0.01. (B) Left, HFL-1 cells were treated with CSE (0, 10 and 20 µg/ml TPM) overnight. MUC1 expression was detected by Western blot. β-Actin was detected as an input control. Middle, HFL-1 cells were treated with CSE (20 µg/ml TPM) for the indicated time points. MUC1 mRNA level was detected by RT-PCR. β-Actin was detected as an input control. Right, HFL-1 cells were transfected with MUC1 siRNA or negative control siRNA for 48 h. MUC1 expression was detected by Western blot with antibody Muc1 GP1.4 against the extracellular domain (MUC1-N) and antibody MUC1 Ab-5 recognizing the C-terminal domain (MUC1-CT). β-Actin was detected as an input control. (C) HFL-1 and Hs888Lu cells were transfected with MUC1 siRNA or negative control siRNA for 24 h, before the cells were treated with CSE (20 µg/ml TPM), TNF-α (10 ng/ml), IL-6 (10 ng/ml) and IL-1β (10 ng/ml) overnight. EREG expression was detected by Western blot. β-Actin was detected as an input control. The intensity of the individual bands was quantified and normalized to the corresponding input control bands. Fold changes were calculated with the control taken as 1.

    Article Snippet: The human lung cancer cell line A549, human fetal lung fibroblast line HFL-1, and human adult lung fibroblast line Hs888Lu were purchased from American Type Culture Collection (ATCC, Manassas, VA).

    Techniques: Expressing, Concentration Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Reverse Transcription Polymerase Chain Reaction, Transfection, Negative Control