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Magainin Pharmaceuticals Inc human β defensin 3
Human β Defensin 3, supplied by Magainin Pharmaceuticals Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human beta defensin 2  (ATCC)
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The modulation of MAPK phosphorylation and wound healing by L. reuteri NCHBL-005. A Expression of phosphorylated p38, ERK, and JNK detected by western blot analysis. B Representative light microscope images from in vitro scratch assays conducted on L929 fibroblasts treated with SB203580, SP600125, and PD0325901 at 24, 48, and 72 h post-treatment. C Quantification of the wound area using Image J, with the scratch area at 0 h (no treatment) set as 100% (mean ± SD). The significance of differences among the groups was assessed using two-way ANOVA, with the level of significance set at * p < 0.05, ** p < 0.01, and *** p < 0.001. Ctrl. Control; LP, L. plantarum NCHBL-004; LK, L. kunkeei NCHBL-003; LR, L. reuteri NCHBL-005; p-p38, phosphorylated p38 mitogen-activated protein kinase; p-JNK, phosphorylated c-jun N-terminal kinase; p-ERK, phosphorylated extracellular signal-regulated kinase; β-actin, <t>beta-actin;</t> SB, SB203580; SP, SP600125; PD, PD0325901; ANOVA, analysis of variance; SD, standard deviation
Human Beta Defensin 2, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human β defensin 2  (RBD Instruments)
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The modulation of MAPK phosphorylation and wound healing by L. reuteri NCHBL-005. A Expression of phosphorylated p38, ERK, and JNK detected by western blot analysis. B Representative light microscope images from in vitro scratch assays conducted on L929 fibroblasts treated with SB203580, SP600125, and PD0325901 at 24, 48, and 72 h post-treatment. C Quantification of the wound area using Image J, with the scratch area at 0 h (no treatment) set as 100% (mean ± SD). The significance of differences among the groups was assessed using two-way ANOVA, with the level of significance set at * p < 0.05, ** p < 0.01, and *** p < 0.001. Ctrl. Control; LP, L. plantarum NCHBL-004; LK, L. kunkeei NCHBL-003; LR, L. reuteri NCHBL-005; p-p38, phosphorylated p38 mitogen-activated protein kinase; p-JNK, phosphorylated c-jun N-terminal kinase; p-ERK, phosphorylated extracellular signal-regulated kinase; β-actin, <t>beta-actin;</t> SB, SB203580; SP, SP600125; PD, PD0325901; ANOVA, analysis of variance; SD, standard deviation
Human β Defensin 2, supplied by RBD Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant human beta defensin 2  (ProSpec)
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ProSpec recombinant human beta defensin 2
The modulation of MAPK phosphorylation and wound healing by L. reuteri NCHBL-005. A Expression of phosphorylated p38, ERK, and JNK detected by western blot analysis. B Representative light microscope images from in vitro scratch assays conducted on L929 fibroblasts treated with SB203580, SP600125, and PD0325901 at 24, 48, and 72 h post-treatment. C Quantification of the wound area using Image J, with the scratch area at 0 h (no treatment) set as 100% (mean ± SD). The significance of differences among the groups was assessed using two-way ANOVA, with the level of significance set at * p < 0.05, ** p < 0.01, and *** p < 0.001. Ctrl. Control; LP, L. plantarum NCHBL-004; LK, L. kunkeei NCHBL-003; LR, L. reuteri NCHBL-005; p-p38, phosphorylated p38 mitogen-activated protein kinase; p-JNK, phosphorylated c-jun N-terminal kinase; p-ERK, phosphorylated extracellular signal-regulated kinase; β-actin, <t>beta-actin;</t> SB, SB203580; SP, SP600125; PD, PD0325901; ANOVA, analysis of variance; SD, standard deviation
Recombinant Human Beta Defensin 2, supplied by ProSpec, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant human beta defensin 2 - by Bioz Stars, 2025-04
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human beta defensin  (Clinical and Laboratory Standards Institute)
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Clinical and Laboratory Standards Institute human beta defensin
The modulation of MAPK phosphorylation and wound healing by L. reuteri NCHBL-005. A Expression of phosphorylated p38, ERK, and JNK detected by western blot analysis. B Representative light microscope images from in vitro scratch assays conducted on L929 fibroblasts treated with SB203580, SP600125, and PD0325901 at 24, 48, and 72 h post-treatment. C Quantification of the wound area using Image J, with the scratch area at 0 h (no treatment) set as 100% (mean ± SD). The significance of differences among the groups was assessed using two-way ANOVA, with the level of significance set at * p < 0.05, ** p < 0.01, and *** p < 0.001. Ctrl. Control; LP, L. plantarum NCHBL-004; LK, L. kunkeei NCHBL-003; LR, L. reuteri NCHBL-005; p-p38, phosphorylated p38 mitogen-activated protein kinase; p-JNK, phosphorylated c-jun N-terminal kinase; p-ERK, phosphorylated extracellular signal-regulated kinase; β-actin, <t>beta-actin;</t> SB, SB203580; SP, SP600125; PD, PD0325901; ANOVA, analysis of variance; SD, standard deviation
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human alpha defensin 1 duoset elisa kit  (R&D Systems)
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R&D Systems human alpha defensin 1 duoset elisa kit
The modulation of MAPK phosphorylation and wound healing by L. reuteri NCHBL-005. A Expression of phosphorylated p38, ERK, and JNK detected by western blot analysis. B Representative light microscope images from in vitro scratch assays conducted on L929 fibroblasts treated with SB203580, SP600125, and PD0325901 at 24, 48, and 72 h post-treatment. C Quantification of the wound area using Image J, with the scratch area at 0 h (no treatment) set as 100% (mean ± SD). The significance of differences among the groups was assessed using two-way ANOVA, with the level of significance set at * p < 0.05, ** p < 0.01, and *** p < 0.001. Ctrl. Control; LP, L. plantarum NCHBL-004; LK, L. kunkeei NCHBL-003; LR, L. reuteri NCHBL-005; p-p38, phosphorylated p38 mitogen-activated protein kinase; p-JNK, phosphorylated c-jun N-terminal kinase; p-ERK, phosphorylated extracellular signal-regulated kinase; β-actin, <t>beta-actin;</t> SB, SB203580; SP, SP600125; PD, PD0325901; ANOVA, analysis of variance; SD, standard deviation
Human Alpha Defensin 1 Duoset Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human alpha defensin  (Genspeed Biotech GmbH)
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The modulation of MAPK phosphorylation and wound healing by L. reuteri NCHBL-005. A Expression of phosphorylated p38, ERK, and JNK detected by western blot analysis. B Representative light microscope images from in vitro scratch assays conducted on L929 fibroblasts treated with SB203580, SP600125, and PD0325901 at 24, 48, and 72 h post-treatment. C Quantification of the wound area using Image J, with the scratch area at 0 h (no treatment) set as 100% (mean ± SD). The significance of differences among the groups was assessed using two-way ANOVA, with the level of significance set at * p < 0.05, ** p < 0.01, and *** p < 0.001. Ctrl. Control; LP, L. plantarum NCHBL-004; LK, L. kunkeei NCHBL-003; LR, L. reuteri NCHBL-005; p-p38, phosphorylated p38 mitogen-activated protein kinase; p-JNK, phosphorylated c-jun N-terminal kinase; p-ERK, phosphorylated extracellular signal-regulated kinase; β-actin, <t>beta-actin;</t> SB, SB203580; SP, SP600125; PD, PD0325901; ANOVA, analysis of variance; SD, standard deviation
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human alpha defensin 1 duoset elisa  (Danaher Inc)
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Danaher Inc human alpha defensin 1 duoset elisa
List of materials used for the detection of human alpha-defensin, calprotectin, and interleukin-6 with the GENSPEED microfluidic test chip.
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recombinant human alpha defensin 1 protein  (Danaher Inc)
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Danaher Inc recombinant human alpha defensin 1 protein
Left panel: Layout of capture antibodies immobilized on the multiplex microfluidic test chip for the respective biomarke. Sample and reagents enter through the chip inlet, pass through a channel with capture antibodies, and exit into the waste reservoir. Right panel: side view showing the sandwich assay setup for each biomarker: immobilized antibodies captured the biomarker, biotinylated detection antibodies bind, and streptavidin-horseradish peroxidase (HRP) interaction enables chemiluminescence detection. A biotinylated control antibody checks the assay’s workflow. α-D, <t>alpha-defensin;</t> CP, calprotecin; Fluo, fluorescein; IL-6, interleukin 6.
Recombinant Human Alpha Defensin 1 Protein, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


The modulation of MAPK phosphorylation and wound healing by L. reuteri NCHBL-005. A Expression of phosphorylated p38, ERK, and JNK detected by western blot analysis. B Representative light microscope images from in vitro scratch assays conducted on L929 fibroblasts treated with SB203580, SP600125, and PD0325901 at 24, 48, and 72 h post-treatment. C Quantification of the wound area using Image J, with the scratch area at 0 h (no treatment) set as 100% (mean ± SD). The significance of differences among the groups was assessed using two-way ANOVA, with the level of significance set at * p < 0.05, ** p < 0.01, and *** p < 0.001. Ctrl. Control; LP, L. plantarum NCHBL-004; LK, L. kunkeei NCHBL-003; LR, L. reuteri NCHBL-005; p-p38, phosphorylated p38 mitogen-activated protein kinase; p-JNK, phosphorylated c-jun N-terminal kinase; p-ERK, phosphorylated extracellular signal-regulated kinase; β-actin, beta-actin; SB, SB203580; SP, SP600125; PD, PD0325901; ANOVA, analysis of variance; SD, standard deviation

Journal: Inflammation and Regeneration

Article Title: Lactobacillus reuteri NCHBL-005 improves wound healing by promoting the activation of fibroblasts through TLR2/MAPK signaling

doi: 10.1186/s41232-025-00370-9

Figure Lengend Snippet: The modulation of MAPK phosphorylation and wound healing by L. reuteri NCHBL-005. A Expression of phosphorylated p38, ERK, and JNK detected by western blot analysis. B Representative light microscope images from in vitro scratch assays conducted on L929 fibroblasts treated with SB203580, SP600125, and PD0325901 at 24, 48, and 72 h post-treatment. C Quantification of the wound area using Image J, with the scratch area at 0 h (no treatment) set as 100% (mean ± SD). The significance of differences among the groups was assessed using two-way ANOVA, with the level of significance set at * p < 0.05, ** p < 0.01, and *** p < 0.001. Ctrl. Control; LP, L. plantarum NCHBL-004; LK, L. kunkeei NCHBL-003; LR, L. reuteri NCHBL-005; p-p38, phosphorylated p38 mitogen-activated protein kinase; p-JNK, phosphorylated c-jun N-terminal kinase; p-ERK, phosphorylated extracellular signal-regulated kinase; β-actin, beta-actin; SB, SB203580; SP, SP600125; PD, PD0325901; ANOVA, analysis of variance; SD, standard deviation

Article Snippet: L. reuteri ATCC-55730 exhibits anti-inflammatory effects by inhibiting the transcription factors for interleukin-8 and human beta-defensin-2 in infected epithelial cells [ ].

Techniques: Expressing, Western Blot, Light Microscopy, In Vitro, Control, Standard Deviation

TLR2-dependent wound healing and MAPK phosphorylation by L. reuteri NCHBL-005. A TLR2 activity of Lactobacilli . B Representative light microscope images from in vitro scratch assays conducted on L929 fibroblasts (0, 24, 48, and 72 h post-treatment). C Quantification of the wound area using Image J, with the scratch area at 0 h (no treatment) set as 100% (mean ± SD). D Representative light microscope images from in vitro scratch assays conducted on MEFs at 0 and 24 h post-treatment. E Quantification of the wound area using Image J, with the scratch area at 0 h (no treatment) set as 100% (mean ± SD). F Expression of phosphorylated p38, ERK, and JNK detected by western blot analysis. The significance of differences among the groups was assessed using two-way ANOVA, with the level of significance set at * p < 0.05 and *** p < 0.001. Ctrl, control; MEF, mouse embryonic fibroblast; WT, wild-type; TLR2, toll-like receptor 2; LR, L. reuteri NCHBL-005; p-p38, phosphorylated p38 mitogen-activated protein kinase; p-JNK, phosphorylated c-jun N-terminal kinase; p-ERK, phosphorylated extracellular signal-regulated kinase; β-actin, beta-actin; ANOVA, analysis of variance; SD, standard deviation

Journal: Inflammation and Regeneration

Article Title: Lactobacillus reuteri NCHBL-005 improves wound healing by promoting the activation of fibroblasts through TLR2/MAPK signaling

doi: 10.1186/s41232-025-00370-9

Figure Lengend Snippet: TLR2-dependent wound healing and MAPK phosphorylation by L. reuteri NCHBL-005. A TLR2 activity of Lactobacilli . B Representative light microscope images from in vitro scratch assays conducted on L929 fibroblasts (0, 24, 48, and 72 h post-treatment). C Quantification of the wound area using Image J, with the scratch area at 0 h (no treatment) set as 100% (mean ± SD). D Representative light microscope images from in vitro scratch assays conducted on MEFs at 0 and 24 h post-treatment. E Quantification of the wound area using Image J, with the scratch area at 0 h (no treatment) set as 100% (mean ± SD). F Expression of phosphorylated p38, ERK, and JNK detected by western blot analysis. The significance of differences among the groups was assessed using two-way ANOVA, with the level of significance set at * p < 0.05 and *** p < 0.001. Ctrl, control; MEF, mouse embryonic fibroblast; WT, wild-type; TLR2, toll-like receptor 2; LR, L. reuteri NCHBL-005; p-p38, phosphorylated p38 mitogen-activated protein kinase; p-JNK, phosphorylated c-jun N-terminal kinase; p-ERK, phosphorylated extracellular signal-regulated kinase; β-actin, beta-actin; ANOVA, analysis of variance; SD, standard deviation

Article Snippet: L. reuteri ATCC-55730 exhibits anti-inflammatory effects by inhibiting the transcription factors for interleukin-8 and human beta-defensin-2 in infected epithelial cells [ ].

Techniques: Activity Assay, Light Microscopy, In Vitro, Expressing, Western Blot, Control, Standard Deviation

List of materials used for the detection of human alpha-defensin, calprotectin, and interleukin-6 with the GENSPEED microfluidic test chip.

Journal: Bone & Joint Research

Article Title: Rapid multiplex micro-ELISA assay for simultaneous measurement of synovial biomarkers

doi: 10.1302/2046-3758.143.BJR-2024-0100.R1

Figure Lengend Snippet: List of materials used for the detection of human alpha-defensin, calprotectin, and interleukin-6 with the GENSPEED microfluidic test chip.

Article Snippet: Alpha-defensin , Human alpha-defensin 1 DuoSet ELISA (R&D Systems, USA) , Human alpha-defensin 1 DuoSet ELISA (R&D Systems) , Recombinant human alpha-defensin 1 protein (Abcam).

Techniques: Enzyme-linked Immunosorbent Assay, Recombinant

Left panel: Layout of capture antibodies immobilized on the multiplex microfluidic test chip for the respective biomarke. Sample and reagents enter through the chip inlet, pass through a channel with capture antibodies, and exit into the waste reservoir. Right panel: side view showing the sandwich assay setup for each biomarker: immobilized antibodies captured the biomarker, biotinylated detection antibodies bind, and streptavidin-horseradish peroxidase (HRP) interaction enables chemiluminescence detection. A biotinylated control antibody checks the assay’s workflow. α-D, alpha-defensin; CP, calprotecin; Fluo, fluorescein; IL-6, interleukin 6.

Journal: Bone & Joint Research

Article Title: Rapid multiplex micro-ELISA assay for simultaneous measurement of synovial biomarkers

doi: 10.1302/2046-3758.143.BJR-2024-0100.R1

Figure Lengend Snippet: Left panel: Layout of capture antibodies immobilized on the multiplex microfluidic test chip for the respective biomarke. Sample and reagents enter through the chip inlet, pass through a channel with capture antibodies, and exit into the waste reservoir. Right panel: side view showing the sandwich assay setup for each biomarker: immobilized antibodies captured the biomarker, biotinylated detection antibodies bind, and streptavidin-horseradish peroxidase (HRP) interaction enables chemiluminescence detection. A biotinylated control antibody checks the assay’s workflow. α-D, alpha-defensin; CP, calprotecin; Fluo, fluorescein; IL-6, interleukin 6.

Article Snippet: Alpha-defensin , Human alpha-defensin 1 DuoSet ELISA (R&D Systems, USA) , Human alpha-defensin 1 DuoSet ELISA (R&D Systems) , Recombinant human alpha-defensin 1 protein (Abcam).

Techniques: Multiplex Assay, Biomarker Assay, Control

List of materials used for the detection of human alpha-defensin, calprotectin, and interleukin-6 with the GENSPEED microfluidic test chip.

Journal: Bone & Joint Research

Article Title: Rapid multiplex micro-ELISA assay for simultaneous measurement of synovial biomarkers

doi: 10.1302/2046-3758.143.BJR-2024-0100.R1

Figure Lengend Snippet: List of materials used for the detection of human alpha-defensin, calprotectin, and interleukin-6 with the GENSPEED microfluidic test chip.

Article Snippet: Alpha-defensin , Human alpha-defensin 1 DuoSet ELISA (R&D Systems, USA) , Human alpha-defensin 1 DuoSet ELISA (R&D Systems) , Recombinant human alpha-defensin 1 protein (Abcam).

Techniques: Enzyme-linked Immunosorbent Assay, Recombinant

List of buffer-based and synovial fluid-based samples with different concentrations of alpha-defensin, calprotectin, and interleukin-6.

Journal: Bone & Joint Research

Article Title: Rapid multiplex micro-ELISA assay for simultaneous measurement of synovial biomarkers

doi: 10.1302/2046-3758.143.BJR-2024-0100.R1

Figure Lengend Snippet: List of buffer-based and synovial fluid-based samples with different concentrations of alpha-defensin, calprotectin, and interleukin-6.

Article Snippet: Alpha-defensin , Human alpha-defensin 1 DuoSet ELISA (R&D Systems, USA) , Human alpha-defensin 1 DuoSet ELISA (R&D Systems) , Recombinant human alpha-defensin 1 protein (Abcam).

Techniques:

Buffer-based dilution series experiment for human alpha-defensin, calprotectin, and interleukin-6 (IL-6) diluted using GENSPEED washing solution measured with multiplex GENSPEED test chips. The figure displays the chemiluminescent signal intensities (AU) across various biomarker concentrations. The data represent mean values from three measurements: a) alpha-defensin; b) calprotectin; c) IL-6; and d) five parameters logistic (PL) regression. PBS, phosphate-buffered saline.

Journal: Bone & Joint Research

Article Title: Rapid multiplex micro-ELISA assay for simultaneous measurement of synovial biomarkers

doi: 10.1302/2046-3758.143.BJR-2024-0100.R1

Figure Lengend Snippet: Buffer-based dilution series experiment for human alpha-defensin, calprotectin, and interleukin-6 (IL-6) diluted using GENSPEED washing solution measured with multiplex GENSPEED test chips. The figure displays the chemiluminescent signal intensities (AU) across various biomarker concentrations. The data represent mean values from three measurements: a) alpha-defensin; b) calprotectin; c) IL-6; and d) five parameters logistic (PL) regression. PBS, phosphate-buffered saline.

Article Snippet: Alpha-defensin , Human alpha-defensin 1 DuoSet ELISA (R&D Systems, USA) , Human alpha-defensin 1 DuoSet ELISA (R&D Systems) , Recombinant human alpha-defensin 1 protein (Abcam).

Techniques: Multiplex Assay, Biomarker Assay, Saline

Dilution series experiments on human synovial fluid spiked with alpha-defension (α-defensin), calprotectin, and interleukin-6 (IL-6) were conducted using multiplex GENSPEED test chips. α-defensin and calprotectin were diluted 1/100, while IL-6 was diluted 1/10 with GENSPEED washing solution. The figure shows chemiluminescent signal intensities (AU) for each biomarker, with all measurements done in triplicates. It includes the limit of detection (LoD) (green line) and a clinically relevant cut-off for periprosthetic joint infection (PJI). Data represent mean values from three tests: a) α-defensin; b) calprotectin; c) interleukin-6; and d) five parameters logistic (PL) regression.

Journal: Bone & Joint Research

Article Title: Rapid multiplex micro-ELISA assay for simultaneous measurement of synovial biomarkers

doi: 10.1302/2046-3758.143.BJR-2024-0100.R1

Figure Lengend Snippet: Dilution series experiments on human synovial fluid spiked with alpha-defension (α-defensin), calprotectin, and interleukin-6 (IL-6) were conducted using multiplex GENSPEED test chips. α-defensin and calprotectin were diluted 1/100, while IL-6 was diluted 1/10 with GENSPEED washing solution. The figure shows chemiluminescent signal intensities (AU) for each biomarker, with all measurements done in triplicates. It includes the limit of detection (LoD) (green line) and a clinically relevant cut-off for periprosthetic joint infection (PJI). Data represent mean values from three tests: a) α-defensin; b) calprotectin; c) interleukin-6; and d) five parameters logistic (PL) regression.

Article Snippet: Alpha-defensin , Human alpha-defensin 1 DuoSet ELISA (R&D Systems, USA) , Human alpha-defensin 1 DuoSet ELISA (R&D Systems) , Recombinant human alpha-defensin 1 protein (Abcam).

Techniques: Multiplex Assay, Biomarker Assay, Infection

Summary of key results evaluating the performance of the multiplex micro-enzyme-linked immunosorbent assay (µ-ELISA) for the simultaneous detection and quantification of alpha-defensin, calprotectin, and interleukin-6 in spiked synovial fluids.

Journal: Bone & Joint Research

Article Title: Rapid multiplex micro-ELISA assay for simultaneous measurement of synovial biomarkers

doi: 10.1302/2046-3758.143.BJR-2024-0100.R1

Figure Lengend Snippet: Summary of key results evaluating the performance of the multiplex micro-enzyme-linked immunosorbent assay (µ-ELISA) for the simultaneous detection and quantification of alpha-defensin, calprotectin, and interleukin-6 in spiked synovial fluids.

Article Snippet: Alpha-defensin , Human alpha-defensin 1 DuoSet ELISA (R&D Systems, USA) , Human alpha-defensin 1 DuoSet ELISA (R&D Systems) , Recombinant human alpha-defensin 1 protein (Abcam).

Techniques: Multiplex Assay