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Univariate and multivariate Cox regression analyses and other clinicopathologic factors of OS in HNSCC.

Journal: PeerJ

Article Title: HtrA3: a promising prognostic biomarker and therapeutic target for head and neck squamous cell carcinoma

doi: 10.7717/peerj.16237

Figure Lengend Snippet: Univariate and multivariate Cox regression analyses and other clinicopathologic factors of OS in HNSCC.

Article Snippet: After blocking with 5% skim milk for 2 h at room temperature, the membrane was incubated with primary antibodies against HtrA1 (55011-1-AP; Proteintech), HtrA2 (15775-1-AP; Proteintech), HtrA3 (bs-18100R; Bioss), HtrA4 (bs-18101R; Bioss), and β -actin (AM1021B, abcepta) at 4 °C overnight and then washed in TBST three times.

Techniques:

(A) Representative immunohistochemistry images of HtrA1-4 in HNSCC and normal tissues (HPA database); (B–C) protein expression of HtrA1 and HtrA3 in HNSCC and normal tissues with datasets from the CPTAC database; expression of HtrA1-4 in BEAS-2B, Cal-27 (D) and Fadu (E) cells obtained by western blot. Values associated with test proteins were normalized to standard β -actin for the relative expression measure; (F–G) column graphs of western blot analysis; (H) HtrA1-4 expression in BEAS-2B, FaDu, and Cal-27 cells by RT-PCR. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Journal: PeerJ

Article Title: HtrA3: a promising prognostic biomarker and therapeutic target for head and neck squamous cell carcinoma

doi: 10.7717/peerj.16237

Figure Lengend Snippet: (A) Representative immunohistochemistry images of HtrA1-4 in HNSCC and normal tissues (HPA database); (B–C) protein expression of HtrA1 and HtrA3 in HNSCC and normal tissues with datasets from the CPTAC database; expression of HtrA1-4 in BEAS-2B, Cal-27 (D) and Fadu (E) cells obtained by western blot. Values associated with test proteins were normalized to standard β -actin for the relative expression measure; (F–G) column graphs of western blot analysis; (H) HtrA1-4 expression in BEAS-2B, FaDu, and Cal-27 cells by RT-PCR. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: After blocking with 5% skim milk for 2 h at room temperature, the membrane was incubated with primary antibodies against HtrA1 (55011-1-AP; Proteintech), HtrA2 (15775-1-AP; Proteintech), HtrA3 (bs-18100R; Bioss), HtrA4 (bs-18101R; Bioss), and β -actin (AM1021B, abcepta) at 4 °C overnight and then washed in TBST three times.

Techniques: Immunohistochemistry, Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction

Univariate and multivariate Cox regression analyses and other clinicopathologic factors of OS in HNSCC.

Journal: PeerJ

Article Title: HtrA3: a promising prognostic biomarker and therapeutic target for head and neck squamous cell carcinoma

doi: 10.7717/peerj.16237

Figure Lengend Snippet: Univariate and multivariate Cox regression analyses and other clinicopathologic factors of OS in HNSCC.

Article Snippet: After blocking with 5% skim milk for 2 h at room temperature, the membrane was incubated with primary antibodies against HtrA1 (55011-1-AP; Proteintech), HtrA2 (15775-1-AP; Proteintech), HtrA3 (bs-18100R; Bioss), HtrA4 (bs-18101R; Bioss), and β -actin (AM1021B, abcepta) at 4 °C overnight and then washed in TBST three times.

Techniques: