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FPM triggered the NF-κB-pathway for inflammation response in HEK-Blue cells. ( a ) NF-κB activation in HEK-Blue Null2 cells with FPM treatment and TNF-α stress. ( b ) NF-κB activation in HEK-Blue <t>hTLR4</t> cells with FPM treatment and TNF-α stress. ( c ) NF-κB activation in HEK-Blue hTLR4 cells with FPM treatment and LPS stress. Three independent experiments in triplicates ( n = 9). **** P < 0.0001, *** P < 0.001, ** P < 0.01 and ns P ≥ 0.05, compared to stress control (one-way ANOVA).
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Average 86 stars, based on 1 article reviews
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FPM triggered the NF-κB-pathway for inflammation response in HEK-Blue cells. ( a ) NF-κB activation in HEK-Blue Null2 cells with FPM treatment and TNF-α stress. ( b ) NF-κB activation in HEK-Blue hTLR4 cells with FPM treatment and TNF-α stress. ( c ) NF-κB activation in HEK-Blue hTLR4 cells with FPM treatment and LPS stress. Three independent experiments in triplicates ( n = 9). **** P < 0.0001, *** P < 0.001, ** P < 0.01 and ns P ≥ 0.05, compared to stress control (one-way ANOVA).

Journal: Journal of Animal Science and Biotechnology

Article Title: Herbal extract fermented with inherent microbiota improves intestinal health by exerting antioxidant and anti-inflammatory effects in vitro and in vivo

doi: 10.1186/s40104-025-01178-w

Figure Lengend Snippet: FPM triggered the NF-κB-pathway for inflammation response in HEK-Blue cells. ( a ) NF-κB activation in HEK-Blue Null2 cells with FPM treatment and TNF-α stress. ( b ) NF-κB activation in HEK-Blue hTLR4 cells with FPM treatment and TNF-α stress. ( c ) NF-κB activation in HEK-Blue hTLR4 cells with FPM treatment and LPS stress. Three independent experiments in triplicates ( n = 9). **** P < 0.0001, *** P < 0.001, ** P < 0.01 and ns P ≥ 0.05, compared to stress control (one-way ANOVA).

Article Snippet: Additionally, selective antibiotics 1X HEK-Blue Selection were used for the HEK-Blue hTLR4 cell line, and 100 μg/mL Zeocin for the HEK-Blue Null2 cell line (both from InvivoGen).

Techniques: Activation Assay, Control