htert bj1 cells  (ATCC)


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    ATCC htert bj1 cells
    Analysis on the expression of viral and cellular gene products in NSPC/iPSCs. (A) Morphological changes of Towne-infected NSPC/iPSCs were observed under the inverted microscope before infection (a) , 2 dpi (b) , 5 dpi (c) , and 7 dpi (d) . (B) RT-PCR analysis of HCMV-encoding gene expression. Total RNAs isolated from NSPC/iPSCs harvested before (−) HCMV infection or at 1, 2, 3, 5, and 7 dpi with HCMV Towne strain were subjected to RT-PCR assays. GAPDH gene expression was assayed for the control. (C) The kinetics of mRNA expression for IE1, UL89, and UL136 in Towne-infected NSPC/iPSCs was examined by real-time quantitative RT-PCR assay. The mRNA expression was normalized to that of G6PDH gene. Real-time PCR data was analyzed by the 2-ΔΔCT method. The fold induction was calculated as the ratio of mRNA levels detected at each time point to that detected at 1 dpi. The y-axis represents fold induction of IE1 and UL136 mRNA (left y-axis) and UL89 mRNA (right y-axis). (D) Immunoblot analysis of HCMV protein expression in HCMV-infected NSPC/iPSCs. Whole-cell lysates of NSPC/iPSCs harvested before (−) HCMV infection or at 1, 2, 5, and 7 dpi with HCMV Towne strain were separated by SDS-PAGE and analyzed by immunoblotting with antibodies against IE1/IE2, pp65, gB, and α-tubulin. (E) RT-PCR analysis of pluripotency and neural differentiation marker gene expression in HCMV-infected NSPC/iPSCs. (F) Immunoblot analysis of neural differentiation marker protein expression in HCMV-infected NSPC/iPSCs. Whole-cell lysates of NSPC/iPSCs were analyzed by immunoblotting with antibodies against Musashi-1, Pax6, and Nestin. (G) <t>hTERT-BJ1</t> cells inoculated with culture supernatant collected from mock-infected NSPC/iPSCs (upper panel) or Towne HCMV-infected NSPC/iPSCs (lower panel) at 8 dpi were subjected to immunofluorescence test with anti-IE1/IE2 antibody (green). Nuclei were stained with DAPI. Representative results from two independent experiments are shown.
    Htert Bj1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    htert bj1 cells - by Bioz Stars, 2023-11
    93/100 stars

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    1) Product Images from "Human cytomegalovirus induces apoptosis in neural stem/progenitor cells derived from induced pluripotent stem cells by generating mitochondrial dysfunction and endoplasmic reticulum stress"

    Article Title: Human cytomegalovirus induces apoptosis in neural stem/progenitor cells derived from induced pluripotent stem cells by generating mitochondrial dysfunction and endoplasmic reticulum stress

    Journal: Herpesviridae

    doi: 10.1186/2042-4280-4-2

    Analysis on the expression of viral and cellular gene products in NSPC/iPSCs. (A) Morphological changes of Towne-infected NSPC/iPSCs were observed under the inverted microscope before infection (a) , 2 dpi (b) , 5 dpi (c) , and 7 dpi (d) . (B) RT-PCR analysis of HCMV-encoding gene expression. Total RNAs isolated from NSPC/iPSCs harvested before (−) HCMV infection or at 1, 2, 3, 5, and 7 dpi with HCMV Towne strain were subjected to RT-PCR assays. GAPDH gene expression was assayed for the control. (C) The kinetics of mRNA expression for IE1, UL89, and UL136 in Towne-infected NSPC/iPSCs was examined by real-time quantitative RT-PCR assay. The mRNA expression was normalized to that of G6PDH gene. Real-time PCR data was analyzed by the 2-ΔΔCT method. The fold induction was calculated as the ratio of mRNA levels detected at each time point to that detected at 1 dpi. The y-axis represents fold induction of IE1 and UL136 mRNA (left y-axis) and UL89 mRNA (right y-axis). (D) Immunoblot analysis of HCMV protein expression in HCMV-infected NSPC/iPSCs. Whole-cell lysates of NSPC/iPSCs harvested before (−) HCMV infection or at 1, 2, 5, and 7 dpi with HCMV Towne strain were separated by SDS-PAGE and analyzed by immunoblotting with antibodies against IE1/IE2, pp65, gB, and α-tubulin. (E) RT-PCR analysis of pluripotency and neural differentiation marker gene expression in HCMV-infected NSPC/iPSCs. (F) Immunoblot analysis of neural differentiation marker protein expression in HCMV-infected NSPC/iPSCs. Whole-cell lysates of NSPC/iPSCs were analyzed by immunoblotting with antibodies against Musashi-1, Pax6, and Nestin. (G) hTERT-BJ1 cells inoculated with culture supernatant collected from mock-infected NSPC/iPSCs (upper panel) or Towne HCMV-infected NSPC/iPSCs (lower panel) at 8 dpi were subjected to immunofluorescence test with anti-IE1/IE2 antibody (green). Nuclei were stained with DAPI. Representative results from two independent experiments are shown.
    Figure Legend Snippet: Analysis on the expression of viral and cellular gene products in NSPC/iPSCs. (A) Morphological changes of Towne-infected NSPC/iPSCs were observed under the inverted microscope before infection (a) , 2 dpi (b) , 5 dpi (c) , and 7 dpi (d) . (B) RT-PCR analysis of HCMV-encoding gene expression. Total RNAs isolated from NSPC/iPSCs harvested before (−) HCMV infection or at 1, 2, 3, 5, and 7 dpi with HCMV Towne strain were subjected to RT-PCR assays. GAPDH gene expression was assayed for the control. (C) The kinetics of mRNA expression for IE1, UL89, and UL136 in Towne-infected NSPC/iPSCs was examined by real-time quantitative RT-PCR assay. The mRNA expression was normalized to that of G6PDH gene. Real-time PCR data was analyzed by the 2-ΔΔCT method. The fold induction was calculated as the ratio of mRNA levels detected at each time point to that detected at 1 dpi. The y-axis represents fold induction of IE1 and UL136 mRNA (left y-axis) and UL89 mRNA (right y-axis). (D) Immunoblot analysis of HCMV protein expression in HCMV-infected NSPC/iPSCs. Whole-cell lysates of NSPC/iPSCs harvested before (−) HCMV infection or at 1, 2, 5, and 7 dpi with HCMV Towne strain were separated by SDS-PAGE and analyzed by immunoblotting with antibodies against IE1/IE2, pp65, gB, and α-tubulin. (E) RT-PCR analysis of pluripotency and neural differentiation marker gene expression in HCMV-infected NSPC/iPSCs. (F) Immunoblot analysis of neural differentiation marker protein expression in HCMV-infected NSPC/iPSCs. Whole-cell lysates of NSPC/iPSCs were analyzed by immunoblotting with antibodies against Musashi-1, Pax6, and Nestin. (G) hTERT-BJ1 cells inoculated with culture supernatant collected from mock-infected NSPC/iPSCs (upper panel) or Towne HCMV-infected NSPC/iPSCs (lower panel) at 8 dpi were subjected to immunofluorescence test with anti-IE1/IE2 antibody (green). Nuclei were stained with DAPI. Representative results from two independent experiments are shown.

    Techniques Used: Expressing, Infection, Inverted Microscopy, Reverse Transcription Polymerase Chain Reaction, Isolation, Quantitative RT-PCR, Real-time Polymerase Chain Reaction, Western Blot, SDS Page, Marker, Immunofluorescence, Staining

    htert bj1 cells  (ATCC)


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    ATCC htert bj1 cells
    Analysis on the expression of viral and cellular gene products in NSPC/iPSCs. (A) Morphological changes of Towne-infected NSPC/iPSCs were observed under the inverted microscope before infection (a) , 2 dpi (b) , 5 dpi (c) , and 7 dpi (d) . (B) RT-PCR analysis of HCMV-encoding gene expression. Total RNAs isolated from NSPC/iPSCs harvested before (−) HCMV infection or at 1, 2, 3, 5, and 7 dpi with HCMV Towne strain were subjected to RT-PCR assays. GAPDH gene expression was assayed for the control. (C) The kinetics of mRNA expression for IE1, UL89, and UL136 in Towne-infected NSPC/iPSCs was examined by real-time quantitative RT-PCR assay. The mRNA expression was normalized to that of G6PDH gene. Real-time PCR data was analyzed by the 2-ΔΔCT method. The fold induction was calculated as the ratio of mRNA levels detected at each time point to that detected at 1 dpi. The y-axis represents fold induction of IE1 and UL136 mRNA (left y-axis) and UL89 mRNA (right y-axis). (D) Immunoblot analysis of HCMV protein expression in HCMV-infected NSPC/iPSCs. Whole-cell lysates of NSPC/iPSCs harvested before (−) HCMV infection or at 1, 2, 5, and 7 dpi with HCMV Towne strain were separated by SDS-PAGE and analyzed by immunoblotting with antibodies against IE1/IE2, pp65, gB, and α-tubulin. (E) RT-PCR analysis of pluripotency and neural differentiation marker gene expression in HCMV-infected NSPC/iPSCs. (F) Immunoblot analysis of neural differentiation marker protein expression in HCMV-infected NSPC/iPSCs. Whole-cell lysates of NSPC/iPSCs were analyzed by immunoblotting with antibodies against Musashi-1, Pax6, and Nestin. (G) <t>hTERT-BJ1</t> cells inoculated with culture supernatant collected from mock-infected NSPC/iPSCs (upper panel) or Towne HCMV-infected NSPC/iPSCs (lower panel) at 8 dpi were subjected to immunofluorescence test with anti-IE1/IE2 antibody (green). Nuclei were stained with DAPI. Representative results from two independent experiments are shown.
    Htert Bj1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/htert bj1 cells/product/ATCC
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    htert bj1 cells - by Bioz Stars, 2023-11
    93/100 stars

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    1) Product Images from "Human cytomegalovirus induces apoptosis in neural stem/progenitor cells derived from induced pluripotent stem cells by generating mitochondrial dysfunction and endoplasmic reticulum stress"

    Article Title: Human cytomegalovirus induces apoptosis in neural stem/progenitor cells derived from induced pluripotent stem cells by generating mitochondrial dysfunction and endoplasmic reticulum stress

    Journal: Herpesviridae

    doi: 10.1186/2042-4280-4-2

    Analysis on the expression of viral and cellular gene products in NSPC/iPSCs. (A) Morphological changes of Towne-infected NSPC/iPSCs were observed under the inverted microscope before infection (a) , 2 dpi (b) , 5 dpi (c) , and 7 dpi (d) . (B) RT-PCR analysis of HCMV-encoding gene expression. Total RNAs isolated from NSPC/iPSCs harvested before (−) HCMV infection or at 1, 2, 3, 5, and 7 dpi with HCMV Towne strain were subjected to RT-PCR assays. GAPDH gene expression was assayed for the control. (C) The kinetics of mRNA expression for IE1, UL89, and UL136 in Towne-infected NSPC/iPSCs was examined by real-time quantitative RT-PCR assay. The mRNA expression was normalized to that of G6PDH gene. Real-time PCR data was analyzed by the 2-ΔΔCT method. The fold induction was calculated as the ratio of mRNA levels detected at each time point to that detected at 1 dpi. The y-axis represents fold induction of IE1 and UL136 mRNA (left y-axis) and UL89 mRNA (right y-axis). (D) Immunoblot analysis of HCMV protein expression in HCMV-infected NSPC/iPSCs. Whole-cell lysates of NSPC/iPSCs harvested before (−) HCMV infection or at 1, 2, 5, and 7 dpi with HCMV Towne strain were separated by SDS-PAGE and analyzed by immunoblotting with antibodies against IE1/IE2, pp65, gB, and α-tubulin. (E) RT-PCR analysis of pluripotency and neural differentiation marker gene expression in HCMV-infected NSPC/iPSCs. (F) Immunoblot analysis of neural differentiation marker protein expression in HCMV-infected NSPC/iPSCs. Whole-cell lysates of NSPC/iPSCs were analyzed by immunoblotting with antibodies against Musashi-1, Pax6, and Nestin. (G) hTERT-BJ1 cells inoculated with culture supernatant collected from mock-infected NSPC/iPSCs (upper panel) or Towne HCMV-infected NSPC/iPSCs (lower panel) at 8 dpi were subjected to immunofluorescence test with anti-IE1/IE2 antibody (green). Nuclei were stained with DAPI. Representative results from two independent experiments are shown.
    Figure Legend Snippet: Analysis on the expression of viral and cellular gene products in NSPC/iPSCs. (A) Morphological changes of Towne-infected NSPC/iPSCs were observed under the inverted microscope before infection (a) , 2 dpi (b) , 5 dpi (c) , and 7 dpi (d) . (B) RT-PCR analysis of HCMV-encoding gene expression. Total RNAs isolated from NSPC/iPSCs harvested before (−) HCMV infection or at 1, 2, 3, 5, and 7 dpi with HCMV Towne strain were subjected to RT-PCR assays. GAPDH gene expression was assayed for the control. (C) The kinetics of mRNA expression for IE1, UL89, and UL136 in Towne-infected NSPC/iPSCs was examined by real-time quantitative RT-PCR assay. The mRNA expression was normalized to that of G6PDH gene. Real-time PCR data was analyzed by the 2-ΔΔCT method. The fold induction was calculated as the ratio of mRNA levels detected at each time point to that detected at 1 dpi. The y-axis represents fold induction of IE1 and UL136 mRNA (left y-axis) and UL89 mRNA (right y-axis). (D) Immunoblot analysis of HCMV protein expression in HCMV-infected NSPC/iPSCs. Whole-cell lysates of NSPC/iPSCs harvested before (−) HCMV infection or at 1, 2, 5, and 7 dpi with HCMV Towne strain were separated by SDS-PAGE and analyzed by immunoblotting with antibodies against IE1/IE2, pp65, gB, and α-tubulin. (E) RT-PCR analysis of pluripotency and neural differentiation marker gene expression in HCMV-infected NSPC/iPSCs. (F) Immunoblot analysis of neural differentiation marker protein expression in HCMV-infected NSPC/iPSCs. Whole-cell lysates of NSPC/iPSCs were analyzed by immunoblotting with antibodies against Musashi-1, Pax6, and Nestin. (G) hTERT-BJ1 cells inoculated with culture supernatant collected from mock-infected NSPC/iPSCs (upper panel) or Towne HCMV-infected NSPC/iPSCs (lower panel) at 8 dpi were subjected to immunofluorescence test with anti-IE1/IE2 antibody (green). Nuclei were stained with DAPI. Representative results from two independent experiments are shown.

    Techniques Used: Expressing, Infection, Inverted Microscopy, Reverse Transcription Polymerase Chain Reaction, Isolation, Quantitative RT-PCR, Real-time Polymerase Chain Reaction, Western Blot, SDS Page, Marker, Immunofluorescence, Staining

    htert bj1 cells  (ATCC)


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    ATCC htert bj1 cells
    Htert Bj1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    htert bj1 cells  (ATCC)


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    ATCC htert bj1 cells
    Htert Bj1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/htert bj1 cells/product/ATCC
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    htert bj1 cells  (ATCC)


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    ATCC htert bj1 cells
    Effects of TPP derivatives on cell viability and intracellular ATP levels in normal fibroblasts <t>(hTERT-BJ1)</t> and human breast cancer cells (MCF-7). Cell viability and intracellular ATP levels were determined in the same treated samples. Hoechst staining (%) of hTERT-BJ1 human fibroblasts (black); ATP level (%) of hTERT-BJ1 human fibroblasts (dotted); Hoechst staining (%) of MCF-7 cells (inclined lines); ATP level (%) of of MCF-7 cells (white). TPP treatments at 1 µM, 72h. Data are represented as mean +/- SEM. *p < 0.05; **p < 0.01; indicates significance, all relative to the control.
    Htert Bj1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/htert bj1 cells/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    htert bj1 cells - by Bioz Stars, 2023-11
    86/100 stars

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    1) Product Images from "Exploiting mitochondrial targeting signal(s), TPP and bis-TPP, for eradicating cancer stem cells (CSCs)"

    Article Title: Exploiting mitochondrial targeting signal(s), TPP and bis-TPP, for eradicating cancer stem cells (CSCs)

    Journal: Aging (Albany NY)

    doi: 10.18632/aging.101384

    Effects of TPP derivatives on cell viability and intracellular ATP levels in normal fibroblasts (hTERT-BJ1) and human breast cancer cells (MCF-7). Cell viability and intracellular ATP levels were determined in the same treated samples. Hoechst staining (%) of hTERT-BJ1 human fibroblasts (black); ATP level (%) of hTERT-BJ1 human fibroblasts (dotted); Hoechst staining (%) of MCF-7 cells (inclined lines); ATP level (%) of of MCF-7 cells (white). TPP treatments at 1 µM, 72h. Data are represented as mean +/- SEM. *p < 0.05; **p < 0.01; indicates significance, all relative to the control.
    Figure Legend Snippet: Effects of TPP derivatives on cell viability and intracellular ATP levels in normal fibroblasts (hTERT-BJ1) and human breast cancer cells (MCF-7). Cell viability and intracellular ATP levels were determined in the same treated samples. Hoechst staining (%) of hTERT-BJ1 human fibroblasts (black); ATP level (%) of hTERT-BJ1 human fibroblasts (dotted); Hoechst staining (%) of MCF-7 cells (inclined lines); ATP level (%) of of MCF-7 cells (white). TPP treatments at 1 µM, 72h. Data are represented as mean +/- SEM. *p < 0.05; **p < 0.01; indicates significance, all relative to the control.

    Techniques Used: Staining

    htert bj1 cells  (ATCC)


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    ATCC htert bj1 cells
    Effects of TPP derivatives on cell viability and intracellular ATP levels in normal fibroblasts <t>(hTERT-BJ1)</t> and human breast cancer cells (MCF-7). Cell viability and intracellular ATP levels were determined in the same treated samples. Hoechst staining (%) of hTERT-BJ1 human fibroblasts (black); ATP level (%) of hTERT-BJ1 human fibroblasts (dotted); Hoechst staining (%) of MCF-7 cells (inclined lines); ATP level (%) of of MCF-7 cells (white). TPP treatments at 1 µM, 72h. Data are represented as mean +/- SEM. *p < 0.05; **p < 0.01; indicates significance, all relative to the control.
    Htert Bj1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/htert bj1 cells/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    htert bj1 cells - by Bioz Stars, 2023-11
    86/100 stars

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    1) Product Images from "Exploiting mitochondrial targeting signal(s), TPP and bis-TPP, for eradicating cancer stem cells (CSCs)"

    Article Title: Exploiting mitochondrial targeting signal(s), TPP and bis-TPP, for eradicating cancer stem cells (CSCs)

    Journal: Aging (Albany NY)

    doi: 10.18632/aging.101384

    Effects of TPP derivatives on cell viability and intracellular ATP levels in normal fibroblasts (hTERT-BJ1) and human breast cancer cells (MCF-7). Cell viability and intracellular ATP levels were determined in the same treated samples. Hoechst staining (%) of hTERT-BJ1 human fibroblasts (black); ATP level (%) of hTERT-BJ1 human fibroblasts (dotted); Hoechst staining (%) of MCF-7 cells (inclined lines); ATP level (%) of of MCF-7 cells (white). TPP treatments at 1 µM, 72h. Data are represented as mean +/- SEM. *p < 0.05; **p < 0.01; indicates significance, all relative to the control.
    Figure Legend Snippet: Effects of TPP derivatives on cell viability and intracellular ATP levels in normal fibroblasts (hTERT-BJ1) and human breast cancer cells (MCF-7). Cell viability and intracellular ATP levels were determined in the same treated samples. Hoechst staining (%) of hTERT-BJ1 human fibroblasts (black); ATP level (%) of hTERT-BJ1 human fibroblasts (dotted); Hoechst staining (%) of MCF-7 cells (inclined lines); ATP level (%) of of MCF-7 cells (white). TPP treatments at 1 µM, 72h. Data are represented as mean +/- SEM. *p < 0.05; **p < 0.01; indicates significance, all relative to the control.

    Techniques Used: Staining

    htert bj1 cells  (ATCC)


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    ATCC htert bj1 cells
    Htert Bj1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    htert bj1 cells  (ATCC)


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    ATCC htert bj1 cells
    Htert Bj1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    htert bj1 cells  (ATCC)


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    ATCC htert bj1 cells
    Htert Bj1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC htert bj1 cells
    Analysis on the expression of viral and cellular gene products in NSPC/iPSCs. (A) Morphological changes of Towne-infected NSPC/iPSCs were observed under the inverted microscope before infection (a) , 2 dpi (b) , 5 dpi (c) , and 7 dpi (d) . (B) RT-PCR analysis of HCMV-encoding gene expression. Total RNAs isolated from NSPC/iPSCs harvested before (−) HCMV infection or at 1, 2, 3, 5, and 7 dpi with HCMV Towne strain were subjected to RT-PCR assays. GAPDH gene expression was assayed for the control. (C) The kinetics of mRNA expression for IE1, UL89, and UL136 in Towne-infected NSPC/iPSCs was examined by real-time quantitative RT-PCR assay. The mRNA expression was normalized to that of G6PDH gene. Real-time PCR data was analyzed by the 2-ΔΔCT method. The fold induction was calculated as the ratio of mRNA levels detected at each time point to that detected at 1 dpi. The y-axis represents fold induction of IE1 and UL136 mRNA (left y-axis) and UL89 mRNA (right y-axis). (D) Immunoblot analysis of HCMV protein expression in HCMV-infected NSPC/iPSCs. Whole-cell lysates of NSPC/iPSCs harvested before (−) HCMV infection or at 1, 2, 5, and 7 dpi with HCMV Towne strain were separated by SDS-PAGE and analyzed by immunoblotting with antibodies against IE1/IE2, pp65, gB, and α-tubulin. (E) RT-PCR analysis of pluripotency and neural differentiation marker gene expression in HCMV-infected NSPC/iPSCs. (F) Immunoblot analysis of neural differentiation marker protein expression in HCMV-infected NSPC/iPSCs. Whole-cell lysates of NSPC/iPSCs were analyzed by immunoblotting with antibodies against Musashi-1, Pax6, and Nestin. (G) <t>hTERT-BJ1</t> cells inoculated with culture supernatant collected from mock-infected NSPC/iPSCs (upper panel) or Towne HCMV-infected NSPC/iPSCs (lower panel) at 8 dpi were subjected to immunofluorescence test with anti-IE1/IE2 antibody (green). Nuclei were stained with DAPI. Representative results from two independent experiments are shown.
    Htert Bj1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/htert bj1 cells/product/ATCC
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    htert bj1 cells - by Bioz Stars, 2023-11
    93/100 stars
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    Analysis on the expression of viral and cellular gene products in NSPC/iPSCs. (A) Morphological changes of Towne-infected NSPC/iPSCs were observed under the inverted microscope before infection (a) , 2 dpi (b) , 5 dpi (c) , and 7 dpi (d) . (B) RT-PCR analysis of HCMV-encoding gene expression. Total RNAs isolated from NSPC/iPSCs harvested before (−) HCMV infection or at 1, 2, 3, 5, and 7 dpi with HCMV Towne strain were subjected to RT-PCR assays. GAPDH gene expression was assayed for the control. (C) The kinetics of mRNA expression for IE1, UL89, and UL136 in Towne-infected NSPC/iPSCs was examined by real-time quantitative RT-PCR assay. The mRNA expression was normalized to that of G6PDH gene. Real-time PCR data was analyzed by the 2-ΔΔCT method. The fold induction was calculated as the ratio of mRNA levels detected at each time point to that detected at 1 dpi. The y-axis represents fold induction of IE1 and UL136 mRNA (left y-axis) and UL89 mRNA (right y-axis). (D) Immunoblot analysis of HCMV protein expression in HCMV-infected NSPC/iPSCs. Whole-cell lysates of NSPC/iPSCs harvested before (−) HCMV infection or at 1, 2, 5, and 7 dpi with HCMV Towne strain were separated by SDS-PAGE and analyzed by immunoblotting with antibodies against IE1/IE2, pp65, gB, and α-tubulin. (E) RT-PCR analysis of pluripotency and neural differentiation marker gene expression in HCMV-infected NSPC/iPSCs. (F) Immunoblot analysis of neural differentiation marker protein expression in HCMV-infected NSPC/iPSCs. Whole-cell lysates of NSPC/iPSCs were analyzed by immunoblotting with antibodies against Musashi-1, Pax6, and Nestin. (G) hTERT-BJ1 cells inoculated with culture supernatant collected from mock-infected NSPC/iPSCs (upper panel) or Towne HCMV-infected NSPC/iPSCs (lower panel) at 8 dpi were subjected to immunofluorescence test with anti-IE1/IE2 antibody (green). Nuclei were stained with DAPI. Representative results from two independent experiments are shown.

    Journal: Herpesviridae

    Article Title: Human cytomegalovirus induces apoptosis in neural stem/progenitor cells derived from induced pluripotent stem cells by generating mitochondrial dysfunction and endoplasmic reticulum stress

    doi: 10.1186/2042-4280-4-2

    Figure Lengend Snippet: Analysis on the expression of viral and cellular gene products in NSPC/iPSCs. (A) Morphological changes of Towne-infected NSPC/iPSCs were observed under the inverted microscope before infection (a) , 2 dpi (b) , 5 dpi (c) , and 7 dpi (d) . (B) RT-PCR analysis of HCMV-encoding gene expression. Total RNAs isolated from NSPC/iPSCs harvested before (−) HCMV infection or at 1, 2, 3, 5, and 7 dpi with HCMV Towne strain were subjected to RT-PCR assays. GAPDH gene expression was assayed for the control. (C) The kinetics of mRNA expression for IE1, UL89, and UL136 in Towne-infected NSPC/iPSCs was examined by real-time quantitative RT-PCR assay. The mRNA expression was normalized to that of G6PDH gene. Real-time PCR data was analyzed by the 2-ΔΔCT method. The fold induction was calculated as the ratio of mRNA levels detected at each time point to that detected at 1 dpi. The y-axis represents fold induction of IE1 and UL136 mRNA (left y-axis) and UL89 mRNA (right y-axis). (D) Immunoblot analysis of HCMV protein expression in HCMV-infected NSPC/iPSCs. Whole-cell lysates of NSPC/iPSCs harvested before (−) HCMV infection or at 1, 2, 5, and 7 dpi with HCMV Towne strain were separated by SDS-PAGE and analyzed by immunoblotting with antibodies against IE1/IE2, pp65, gB, and α-tubulin. (E) RT-PCR analysis of pluripotency and neural differentiation marker gene expression in HCMV-infected NSPC/iPSCs. (F) Immunoblot analysis of neural differentiation marker protein expression in HCMV-infected NSPC/iPSCs. Whole-cell lysates of NSPC/iPSCs were analyzed by immunoblotting with antibodies against Musashi-1, Pax6, and Nestin. (G) hTERT-BJ1 cells inoculated with culture supernatant collected from mock-infected NSPC/iPSCs (upper panel) or Towne HCMV-infected NSPC/iPSCs (lower panel) at 8 dpi were subjected to immunofluorescence test with anti-IE1/IE2 antibody (green). Nuclei were stained with DAPI. Representative results from two independent experiments are shown.

    Article Snippet: HCMV laboratory strain Towne (ATCC VR-977) was propagated in hTERT-BJ1 cells.

    Techniques: Expressing, Infection, Inverted Microscopy, Reverse Transcription Polymerase Chain Reaction, Isolation, Quantitative RT-PCR, Real-time Polymerase Chain Reaction, Western Blot, SDS Page, Marker, Immunofluorescence, Staining