heteromeric 5 ht2ar mglu2r complex  (Expression Systems Inc)

Journal: Journal of Neurochemistry

Article Title: Serotonergic Psychedelics Rapidly Modulate Evoked Glutamate Release in Cultured Cortical Neurons

doi: 10.1111/jnc.70020

Figure Lengend Snippet: 5‐HT2AR antagonists ketanserin and M100907, as well as the nonselective 5‐HT2AR agonist lisuride, affect SV pools. Representative colour gradient images (range shown in the rectangle below) from live‐cell imaging of sypHy‐expressing neurons treated with vehicle (CTRL) or ketanserin (KTSR) 1 or 5 μM (a), CTRL or M100907 1 or 10 μM (e), and CTRL, serotonin (SER) 10 or 100 μM or lisuride 10 μM (i). Images show cells during baseline recording, stimulation with 40 and 900 APs at 20 Hz and upon NH 4 Cl pulse, in the presence of bafilomycin A1. Scale bar is 10 μM. (b, f, j) Average traces of sypHy fluorescence intensity normalised to minimum (baseline, F 0 ) and maximum (NH 4 Cl pulse, F max ). Total number of recordings per treatment from 3 independent culture preparations is indicated in brackets. Quantification of the RRP (c, g, k) and TRP (d, h, l) fractions of SVs from the respective experiments. In graphs, bars show the mean, whiskers SEM and circles all data points. Statistical significance was assessed by one‐way ANOVA (KTSR: RRP: F (2,24) = 3.294, p = 0.054, TRP: F (2,24) = 21.59, p < 0.001; M100907: RRP: F (2,28) = 3.476, p = 0.045, TRP: F (2,28) = 4.757, p = 0.017; SER + LIS: RRP: F (3,48) = 0.353, p = 0.787, TRP: F (3,48) = 16.12, p < 0.001) with Dunnett's multiple comparisons test CTRL versus each drug (KTSR: TRP: 1 μM p = 0.676, 5 μM p < 0.001; M100907: RRP: 1 μM p = 0.212, 10 μM p = 0.029, TRP: 1 μM p = 0.242, 10 μM p = 0.010; SER + LIS: TRP: SER 10 μM p = 0.829, SER 100 μM p = 0.189, LIS p < 0.001).

Article Snippet: For treatment of cells, the following drugs were used: lysergic acid diethylamide (LSD) fumarate (custom synthesis, Alfarma), N,N ‐dimethyltryptamine (DMT) fumarate (cat. no. 9003568, Cayman Chemical), 4‐hydroxydimethyltryptamine (psilocin, cat. no. 11864, Cayman Chemical), all diluted in 5% DMSO; 5‐HT2AR antagonists M100907 (volinanserin; cat. no. T5389, TargetMol) diluted in DMSO and ketanserin tartrate (cat. no. AG‐CR1‐0014‐M010, AdipoGen Life Sciences) in 2.5% DMSO, serotonin hydrochloride (cat. no. H9523, Sigma‐Aldrich) in H 2 O, lisuride maleate (cat. no. 4052, Tocris), 5‐HT7 rec. antagonist DR4485 hydrochloride (cat. no. 5005, Tocris) in 2% DMSO and 5‐HT1A rec. antagonist NAD299 hydrochloride (cat. no. 3282, Tocris) in H 2 O.

Techniques: Live Cell Imaging, Expressing, Fluorescence

Journal: Molecular Medicine

Article Title: Effects of psychedelics on neurogenesis and broader neuroplasticity: a systematic review

doi: 10.1186/s10020-024-01013-4

Figure Lengend Snippet: Results reported by the reviewed studies on psychoactive tryptamines

Article Snippet: Physiological assesment (BrdU + and DCX + cells) Behavioral asessment (MWM; NORT) , Mice (adult males), strain: C57BL6 , - DMT ↑ survival and proliferation of BrdU + and DCX + cells, which is blocked by Sigma-1R antagonist ☆△ - DMT ↓ escape latency in MWM is blocked by 5-HT2AR antagonist - DMT ↑ exploration time in NORT , (Morales-Garcia et al. ) .

Techniques: In Vivo, Membrane, Injection, Activity Assay, Expressing, Control, Ex Vivo, Inhibition, In Vitro, Incubation, Cell Culture, Binding Assay

Journal: Molecular Medicine

Article Title: Effects of psychedelics on neurogenesis and broader neuroplasticity: a systematic review

doi: 10.1186/s10020-024-01013-4

Figure Lengend Snippet: Results reported by the reviewed studies on other psychedelics not covered by previous classifications

Article Snippet: Physiological assesment (BrdU + and DCX + cells) Behavioral asessment (MWM; NORT) , Mice (adult males), strain: C57BL6 , - DMT ↑ survival and proliferation of BrdU + and DCX + cells, which is blocked by Sigma-1R antagonist ☆△ - DMT ↓ escape latency in MWM is blocked by 5-HT2AR antagonist - DMT ↑ exploration time in NORT , (Morales-Garcia et al. ) .

Techniques: In Vivo, Control, Blocking Assay, Membrane, Ex Vivo, Inhibition, In Vitro, Activity Assay, Cell Culture, Residue