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Identification and characterization of hAECs-Exo. (A) Morphology of exosomes in the hAECs-Exo group was observed under transmission electron microscopy (Scalar bar = 200 nm). (B) Diameter of exosomes in the hAECs-Exo group was analysis under NTA. (C) Western blot was used to observe the expression of CD63, CD81, and <t>Grp94</t> proteins in hAECs-Exo. hAECs-Exo = human amniotic epithelial cell-derived exosomes, NTA = nanoparticle tracking analysis.
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Identification and characterization of hAECs-Exo. (A) Morphology of exosomes in the hAECs-Exo group was observed under transmission electron microscopy (Scalar bar = 200 nm). (B) Diameter of exosomes in the hAECs-Exo group was analysis under NTA. (C) Western blot was used to observe the expression of CD63, CD81, and <t>Grp94</t> proteins in hAECs-Exo. hAECs-Exo = human amniotic epithelial cell-derived exosomes, NTA = nanoparticle tracking analysis.
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Identification and characterization of hAECs-Exo. (A) Morphology of exosomes in the hAECs-Exo group was observed under transmission electron microscopy (Scalar bar = 200 nm). (B) Diameter of exosomes in the hAECs-Exo group was analysis under NTA. (C) Western blot was used to observe the expression of CD63, CD81, and <t>Grp94</t> proteins in hAECs-Exo. hAECs-Exo = human amniotic epithelial cell-derived exosomes, NTA = nanoparticle tracking analysis.
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Identification and characterization of hAECs-Exo. (A) Morphology of exosomes in the hAECs-Exo group was observed under transmission electron microscopy (Scalar bar = 200 nm). (B) Diameter of exosomes in the hAECs-Exo group was analysis under NTA. (C) Western blot was used to observe the expression of CD63, CD81, and <t>Grp94</t> proteins in hAECs-Exo. hAECs-Exo = human amniotic epithelial cell-derived exosomes, NTA = nanoparticle tracking analysis.
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Identification and characterization of hAECs-Exo. (A) Morphology of exosomes in the hAECs-Exo group was observed under transmission electron microscopy (Scalar bar = 200 nm). (B) Diameter of exosomes in the hAECs-Exo group was analysis under NTA. (C) Western blot was used to observe the expression of CD63, CD81, and <t>Grp94</t> proteins in hAECs-Exo. hAECs-Exo = human amniotic epithelial cell-derived exosomes, NTA = nanoparticle tracking analysis.
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rabbit anti grp94 - by Bioz Stars, 2026-11
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antibodies against positive ev markers  (Boster Bio)
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Identification and characterization of hAECs-Exo. (A) Morphology of exosomes in the hAECs-Exo group was observed under transmission electron microscopy (Scalar bar = 200 nm). (B) Diameter of exosomes in the hAECs-Exo group was analysis under NTA. (C) Western blot was used to observe the expression of CD63, CD81, and <t>Grp94</t> proteins in hAECs-Exo. hAECs-Exo = human amniotic epithelial cell-derived exosomes, NTA = nanoparticle tracking analysis.
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Identification and characterization of hAECs-Exo. (A) Morphology of exosomes in the hAECs-Exo group was observed under transmission electron microscopy (Scalar bar = 200 nm). (B) Diameter of exosomes in the hAECs-Exo group was analysis under NTA. (C) Western blot was used to observe the expression of CD63, CD81, and Grp94 proteins in hAECs-Exo. hAECs-Exo = human amniotic epithelial cell-derived exosomes, NTA = nanoparticle tracking analysis.

Journal: Medicine

Article Title: Human amniotic epithelial cell-derived exosomes promote conjunctival goblet cells proliferation and mucin secretion

doi: 10.1097/MD.0000000000049647

Figure Lengend Snippet: Identification and characterization of hAECs-Exo. (A) Morphology of exosomes in the hAECs-Exo group was observed under transmission electron microscopy (Scalar bar = 200 nm). (B) Diameter of exosomes in the hAECs-Exo group was analysis under NTA. (C) Western blot was used to observe the expression of CD63, CD81, and Grp94 proteins in hAECs-Exo. hAECs-Exo = human amniotic epithelial cell-derived exosomes, NTA = nanoparticle tracking analysis.

Article Snippet: The polyvinylidene fluoride membrane was treated with protein-free rapid blocking solution (PS108P, EpiZyme) for 15 minutes, and then incubated with primary antibodies against exosome markers CD63 (1:1000, ABclonal, A19023) and CD81 (1:1000, ABclonal, A4863), as well as the negative marker Grp94 (1:1000, ABclonal, A0989) overnight.

Techniques: Transmission Assay, Electron Microscopy, Western Blot, Expressing, Derivative Assay