hs 68  (ATCC)

Journal: International Journal of Nanomedicine

Article Title: Synergistic Fat-Reducing Effect of Deoxycholic Acid and Rhein in Lipid-Based Nanoparticles with Reduced Toxicity for Obesity Treatment

doi: 10.2147/IJN.S494416

Figure Lengend Snippet: The cell uptake and cell viability after treatment of free DA and/or rhein solution or squarticles loaded with DA and/or rhein: ( A ) flow cytometry analysis of empty or DA-loaded squarticles incubated with 3T3-L1 cells; ( B ) the quantification of intracellular uptake of empty or DA-loaded squarticles in 3T3-L1 cells; ( C ) confocal microscopic images of empty or DA-loaded squarticles (red), DAPI (blue), and LysoTracker (green) showing uptake of nanoparticles by 3T3-L1 cells in 3T3-L1 cells; ( D ) the 3T3-L1 cell viability after treatment of free DA and/or rhein solution or squarticles loaded with DA and/or rhein as determined by MTT assay; and ( E ) the in skin fibroblast (Hs68) viability after treatment of free DA and/or rhein solution or squarticles loaded with DA and/or rhein as determined by MTT assay. All data are presented as the mean of three experiments ± S.E.M. *** p < 0.001; ** p < 0.01; * p < 0.05 as compared to the control group.

Article Snippet: 3T3-L1 preadipocytes and Hs68 skin fibroblasts were purchased from the Bioresource Collection and Research Center, Taiwan.

Techniques: Flow Cytometry, Incubation, MTT Assay, Control

Journal: International Journal of Nanomedicine

Article Title: Synergistic Fat-Reducing Effect of Deoxycholic Acid and Rhein in Lipid-Based Nanoparticles with Reduced Toxicity for Obesity Treatment

doi: 10.2147/IJN.S494416

Figure Lengend Snippet: The effect of combined DA and rhein in free or nanoparticulate form on apoptosis and necrosis of 3T3-L1 cells and skin fibroblasts (Hs68): ( A ) 3T3-L1 cell death mechanism analysis using Annexin V and Propidium iodide through flow cytometry; ( B ) skin fibroblast death mechanism analysis using Annexin V and Propidium iodide through flow cytometry; and ( C ) Western blotting assay of caspase 3, caspase 9, and PARP in 3T3-L1 cells after treatment with combined DA and rhein in free or nanoparticulate form. All data are presented as the mean of three experiments ± S.E.M. ** p < 0.01 between the free and nanoparticulate forms ( A and B ). ** p < 0.01 and * p < 0.05 as compared to the MDI-treatment group (mature adipocytes) ( C ).

Article Snippet: 3T3-L1 preadipocytes and Hs68 skin fibroblasts were purchased from the Bioresource Collection and Research Center, Taiwan.

Techniques: Flow Cytometry, Western Blot

Journal: Materials Today Bio

Article Title: Extracellular vesicles from human adipose-derived stem cell spheroids: Characterization and therapeutic implications in diabetic wound healing

doi: 10.1016/j.mtbio.2024.101333

Figure Lengend Snippet: hASCs cultured in microwells at different seeding densities to form different-sized spheroids. A Representative microscopic images of hASC spheroids formed by respective cell number (2 K, 4 K, and 8 K; scale bar = 200 μm). B Ferret diameter of 2 K, 4 K, and 8 K spheroids was estimated at days 0 and 1 (n = 3; ∗ p < 0.05 relative to the 2 K spheroid group). C Cell viability in HS68 fibroblast incubated with the EV groups at 50 μg/ml (n = 4). D hASC EV size distribution from 2 K, 4 K, and 8 K cell spheroid. E hASC EV mean size from 2 K, 4 K and 8 K cell spheroid. F hASC EV number per cell produced from 2 K, 4 K, and 8 K cell spheroid. G hASC EV yield per million cells from 2 K, 4 K, and 8 K cell spheroid. (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, relative to the 2k spheroid hASC group).

Article Snippet: HUVECs (endothelial cells) and HS68 (fibroblast cells) (Bioresource Collection & Research Center, Hsinchu, Taiwan) were incubated in EBM2 supplemented and DMEM-HG, supplemented, respectively.

Techniques: Cell Culture, Incubation, Produced

Journal: Materials Today Bio

Article Title: Extracellular vesicles from human adipose-derived stem cell spheroids: Characterization and therapeutic implications in diabetic wound healing

doi: 10.1016/j.mtbio.2024.101333

Figure Lengend Snippet: EVs enhanced HS68 fibroblast cell migration in vitro. A Cell scratch assay as observed under a light microscope at different time periods (scale bar: 500 μm) B The scratch closure rate analysis presented over time (n = 3) C Cell viability in HUVECs incubated with the different EV groups at a 50 μg/ml (n = 4). D Cell viability in HS68 fibroblast incubated with the EV groups at different concentrations (n = 4). (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, relative to the control group). (#P < 0.05, ##P < 0.01, ###P < 0.001, relative to the monolayer group).

Article Snippet: HUVECs (endothelial cells) and HS68 (fibroblast cells) (Bioresource Collection & Research Center, Hsinchu, Taiwan) were incubated in EBM2 supplemented and DMEM-HG, supplemented, respectively.

Techniques: Migration, In Vitro, Wound Healing Assay, Light Microscopy, Incubation, Control

Journal: Heliyon

Article Title: Enhancing docetaxel efficacy and reducing toxicity using biodegradable periodic mesoporous organosilica nanoparticles

doi: 10.1016/j.heliyon.2024.e40131

Figure Lengend Snippet: The viability (%) of (A) Hs68 fibroblast cells; (B) VcaP prostate cancer cells; (C) A549 human lung adenocarcinoma cells and (D) MCF-7 breast cancer cells treated with BPMO, MSN, DTX, BPMO@DTX, and MSN@DTX was evaluated using the MTT assay. N = 3. ∗, ∗∗, and ∗∗∗ indicate p < 0.05, <0.01, and <0.001, respectively (Student t-test).

Article Snippet: These analyses used the normal human fibroblast cell line Hs68 (source ATCC, supplier Sigma-Aldrich), the prostate cancer cell line VcAP (source ATCC, supplier Sigma-Aldrich), the human lung adenocarcinoma cell line A549 (source ATCC, supplier Sigma-Aldrich) and the breast cancer cell line MCF-7 (source ATCC, supplier Sigma-Aldrich).

Techniques: MTT Assay

Journal: bioRxiv

Article Title: The Human Cytomegalovirus vGPCR UL33 is Essential for Efficient Lytic Replication in Epithelial Cells

doi: 10.1101/2024.09.18.609710

Figure Lengend Snippet: ( A ) Primary human salivary gland epithelial cells (hSGEs) were infected (MOI= 2.0 IU/cell) with TB40/E- mCherry or TB40/E-mCherry(Δall). Concurrently, equal numbers of HS68 HFF cells were infected with the same amount of virus. At 2 days post-infection (dpi), cells were analyzed by flow cytometry for the proportion of cells which were IE1/2 positive. Infectivity Indices were calculated by taking the percent of IE1/2 positive hSGE cells and dividing by the percent IE1/2 positive HS68 cells from the same experiment. The results are representative of 8 independent experiments with 2 different parotid and 2 different submandibular cell cultures. Error is shown as Standard deviation and significance was calculated using unpaired t-tests. ( B ) Primary hSGE cells derived from 2 different parotid glands and 2 different submandibular glands and were infected (2.0 IU/cell) and supernatants harvested at the indicated dpi. Supernatants were used to calculate viral titers on HS68 HFF cells as described in the materials and methods. The results are representative of 4 independent experiments. Error is shown as Standard deviation and significance was calculated using unpaired t-tests. (C) ARPE-19 or HS68 HFF cells were infected as in panel A and Infectivity Indexes were calculated by taking the percent of IE1/IE2 positive ARPE cells and dividing by the percent of IE1/IE2 positive HS68 cells. The results are representative of 10 independent experiments. Error is shown as Standard deviation and significance was calculated using unpaired t-tests. * p <0.05, ** p <0.01

Article Snippet: ARPE-19 cells are adult human retinal pigment epithelial cells and were maintained in DMEM–F-12 medium (50:50; Corning) with 10% FBS, 1% P/S, and in some cases, 1% amphotericin B. HS68 HFF cells were maintained in DMEM supplemented with 10% FetalClone III serum (HyClone) and 1% P/S.

Techniques: Infection, Virus, Flow Cytometry, Standard Deviation, Derivative Assay

Journal: bioRxiv

Article Title: The Human Cytomegalovirus vGPCR UL33 is Essential for Efficient Lytic Replication in Epithelial Cells

doi: 10.1101/2024.09.18.609710

Figure Lengend Snippet: ( A ) Primary human salivary gland epithelial cells (hSGEs) were infected (MOI= 2.0 IU/cell) with TB40/E- mCherry or TB40/E-mCherry(Δall). Concurrently, equal numbers of HS68 HFF cells were infected with the same amount of virus. At 2 days post-infection (dpi), cells were analyzed by flow cytometry for the proportion of cells which were IE1/2 positive. Infectivity Indices were calculated by taking the percent of IE1/2 positive hSGE cells and dividing by the percent IE1/2 positive HS68 cells from the same experiment. The results are representative of 8 independent experiments with 2 different parotid and 2 different submandibular cell cultures. Error is shown as Standard deviation and significance was calculated using unpaired t-tests. ( B ) Primary hSGE cells derived from 2 different parotid glands and 2 different submandibular glands and were infected (2.0 IU/cell) and supernatants harvested at the indicated dpi. Supernatants were used to calculate viral titers on HS68 HFF cells as described in the materials and methods. The results are representative of 4 independent experiments. Error is shown as Standard deviation and significance was calculated using unpaired t-tests. (C) ARPE-19 or HS68 HFF cells were infected as in panel A and Infectivity Indexes were calculated by taking the percent of IE1/IE2 positive ARPE cells and dividing by the percent of IE1/IE2 positive HS68 cells. The results are representative of 10 independent experiments. Error is shown as Standard deviation and significance was calculated using unpaired t-tests. * p <0.05, ** p <0.01

Article Snippet: 293T, ARPE-19, and HS68 human foreskin fibroblast (HFF) cells were acquired from the American Type Culture Collection (ATCC).

Techniques: Infection, Virus, Flow Cytometry, Standard Deviation, Derivative Assay