hrp conjugated spa  (Boster Bio)


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    Boster Bio hrp conjugated spa
    Identification the binding of pIgG or hIgG to IBPs of S. suis 2 by Far-western blot. SDS-PAGE ( a , c ) and Far-western blot analysis ( b , d ) of the S. suis 2 recombinant IBPs. Recombinant proteins (Enolase, LysM, Pyk, LDH, FBA, KAR) and casein were subjected to SDS-PAGE, then transferred onto PVDF membranes and incubated with pIgG or hIgG. Bound pIgG or hIgG was recognized with <t>HRP</t> conjugated <t>SPA.</t> Casein was used as a negative control for non-specific binding to IgG
    Hrp Conjugated Spa, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated spa/product/Boster Bio
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated spa - by Bioz Stars, 2022-09
    92/100 stars

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    1) Product Images from "Identification of novel pig and human immunoglobulin G-binding proteins and characterization of the binding regions of enolase from Streptococcus suis serotype 2"

    Article Title: Identification of novel pig and human immunoglobulin G-binding proteins and characterization of the binding regions of enolase from Streptococcus suis serotype 2

    Journal: AMB Express

    doi: 10.1186/s13568-020-01042-2

    Identification the binding of pIgG or hIgG to IBPs of S. suis 2 by Far-western blot. SDS-PAGE ( a , c ) and Far-western blot analysis ( b , d ) of the S. suis 2 recombinant IBPs. Recombinant proteins (Enolase, LysM, Pyk, LDH, FBA, KAR) and casein were subjected to SDS-PAGE, then transferred onto PVDF membranes and incubated with pIgG or hIgG. Bound pIgG or hIgG was recognized with HRP conjugated SPA. Casein was used as a negative control for non-specific binding to IgG
    Figure Legend Snippet: Identification the binding of pIgG or hIgG to IBPs of S. suis 2 by Far-western blot. SDS-PAGE ( a , c ) and Far-western blot analysis ( b , d ) of the S. suis 2 recombinant IBPs. Recombinant proteins (Enolase, LysM, Pyk, LDH, FBA, KAR) and casein were subjected to SDS-PAGE, then transferred onto PVDF membranes and incubated with pIgG or hIgG. Bound pIgG or hIgG was recognized with HRP conjugated SPA. Casein was used as a negative control for non-specific binding to IgG

    Techniques Used: Binding Assay, Far Western Blot, SDS Page, Recombinant, Incubation, Negative Control

    Identification the binding of pIgG or hIgG to IBPs of S. suis 2 by dot blot. Recombinant pIBPs ( a ) and hIBPs ( b ) were spotted onto the methanol-activated PVDF membranes and incubated with pIgG or hIgG. Bound pIgG or hIgG was recognized with HRP conjugated SPA. Casein was used as a negative control for non-specific binding to IgG and the membrane with HRP conjugated SPA alone were used as a blank control
    Figure Legend Snippet: Identification the binding of pIgG or hIgG to IBPs of S. suis 2 by dot blot. Recombinant pIBPs ( a ) and hIBPs ( b ) were spotted onto the methanol-activated PVDF membranes and incubated with pIgG or hIgG. Bound pIgG or hIgG was recognized with HRP conjugated SPA. Casein was used as a negative control for non-specific binding to IgG and the membrane with HRP conjugated SPA alone were used as a blank control

    Techniques Used: Binding Assay, Dot Blot, Recombinant, Incubation, Negative Control

    2-DE profiles and Far-western blot to identify the affinity of pIgG and hIgG interactions with S. suis 2 cell wall and extracellular proteins. The cell wall and extracellular proteins of S. suis 2 were loaded into immobilized pH gradient strips for IEF analysis and by SDS-PAGE in the second dimension. The 2-DE gels were transferred onto PVDF membranes and incubated with pIgG or hIgG. Arrows indicate pIBPs or hIBPs detected with HRP conjugated SPA. a 2-DE gel of S. suis 2 extracellular proteins. b Far-western blot of extracellular proteins incubated with pIgG. c Far-western blot of extracellular proteins incubated with hIgG. d 2-DE gel of S. suis 2 cell wall proteins. e Far-western blot of cell wall proteins incubated with pIgG. f Far-western blot of cell wall proteins incubated with hIgG
    Figure Legend Snippet: 2-DE profiles and Far-western blot to identify the affinity of pIgG and hIgG interactions with S. suis 2 cell wall and extracellular proteins. The cell wall and extracellular proteins of S. suis 2 were loaded into immobilized pH gradient strips for IEF analysis and by SDS-PAGE in the second dimension. The 2-DE gels were transferred onto PVDF membranes and incubated with pIgG or hIgG. Arrows indicate pIBPs or hIBPs detected with HRP conjugated SPA. a 2-DE gel of S. suis 2 extracellular proteins. b Far-western blot of extracellular proteins incubated with pIgG. c Far-western blot of extracellular proteins incubated with hIgG. d 2-DE gel of S. suis 2 cell wall proteins. e Far-western blot of cell wall proteins incubated with pIgG. f Far-western blot of cell wall proteins incubated with hIgG

    Techniques Used: Far Western Blot, Electrofocusing, SDS Page, Incubation

    2) Product Images from "Identification of novel pig and human immunoglobulin G-binding proteins and characterization of the binding regions of enolase from Streptococcus suis serotype 2"

    Article Title: Identification of novel pig and human immunoglobulin G-binding proteins and characterization of the binding regions of enolase from Streptococcus suis serotype 2

    Journal: AMB Express

    doi: 10.1186/s13568-020-01042-2

    Identification the binding of pIgG or hIgG to IBPs of S. suis 2 by Far-western blot. SDS-PAGE ( a , c ) and Far-western blot analysis ( b , d ) of the S. suis 2 recombinant IBPs. Recombinant proteins (Enolase, LysM, Pyk, LDH, FBA, KAR) and casein were subjected to SDS-PAGE, then transferred onto PVDF membranes and incubated with pIgG or hIgG. Bound pIgG or hIgG was recognized with HRP conjugated SPA. Casein was used as a negative control for non-specific binding to IgG
    Figure Legend Snippet: Identification the binding of pIgG or hIgG to IBPs of S. suis 2 by Far-western blot. SDS-PAGE ( a , c ) and Far-western blot analysis ( b , d ) of the S. suis 2 recombinant IBPs. Recombinant proteins (Enolase, LysM, Pyk, LDH, FBA, KAR) and casein were subjected to SDS-PAGE, then transferred onto PVDF membranes and incubated with pIgG or hIgG. Bound pIgG or hIgG was recognized with HRP conjugated SPA. Casein was used as a negative control for non-specific binding to IgG

    Techniques Used: Binding Assay, Far Western Blot, SDS Page, Recombinant, Incubation, Negative Control

    Identification the binding of pIgG or hIgG to IBPs of S. suis 2 by dot blot. Recombinant pIBPs ( a ) and hIBPs ( b ) were spotted onto the methanol-activated PVDF membranes and incubated with pIgG or hIgG. Bound pIgG or hIgG was recognized with HRP conjugated SPA. Casein was used as a negative control for non-specific binding to IgG and the membrane with HRP conjugated SPA alone were used as a blank control
    Figure Legend Snippet: Identification the binding of pIgG or hIgG to IBPs of S. suis 2 by dot blot. Recombinant pIBPs ( a ) and hIBPs ( b ) were spotted onto the methanol-activated PVDF membranes and incubated with pIgG or hIgG. Bound pIgG or hIgG was recognized with HRP conjugated SPA. Casein was used as a negative control for non-specific binding to IgG and the membrane with HRP conjugated SPA alone were used as a blank control

    Techniques Used: Binding Assay, Dot Blot, Recombinant, Incubation, Negative Control

    2-DE profiles and Far-western blot to identify the affinity of pIgG and hIgG interactions with S. suis 2 cell wall and extracellular proteins. The cell wall and extracellular proteins of S. suis 2 were loaded into immobilized pH gradient strips for IEF analysis and by SDS-PAGE in the second dimension. The 2-DE gels were transferred onto PVDF membranes and incubated with pIgG or hIgG. Arrows indicate pIBPs or hIBPs detected with HRP conjugated SPA. a 2-DE gel of S. suis 2 extracellular proteins. b Far-western blot of extracellular proteins incubated with pIgG. c Far-western blot of extracellular proteins incubated with hIgG. d 2-DE gel of S. suis 2 cell wall proteins. e Far-western blot of cell wall proteins incubated with pIgG. f Far-western blot of cell wall proteins incubated with hIgG
    Figure Legend Snippet: 2-DE profiles and Far-western blot to identify the affinity of pIgG and hIgG interactions with S. suis 2 cell wall and extracellular proteins. The cell wall and extracellular proteins of S. suis 2 were loaded into immobilized pH gradient strips for IEF analysis and by SDS-PAGE in the second dimension. The 2-DE gels were transferred onto PVDF membranes and incubated with pIgG or hIgG. Arrows indicate pIBPs or hIBPs detected with HRP conjugated SPA. a 2-DE gel of S. suis 2 extracellular proteins. b Far-western blot of extracellular proteins incubated with pIgG. c Far-western blot of extracellular proteins incubated with hIgG. d 2-DE gel of S. suis 2 cell wall proteins. e Far-western blot of cell wall proteins incubated with pIgG. f Far-western blot of cell wall proteins incubated with hIgG

    Techniques Used: Far Western Blot, Electrofocusing, SDS Page, Incubation

    3) Product Images from "Identification of novel pig and human immunoglobulin G-binding proteins and characterization of the binding regions of enolase from Streptococcus suis serotype 2"

    Article Title: Identification of novel pig and human immunoglobulin G-binding proteins and characterization of the binding regions of enolase from Streptococcus suis serotype 2

    Journal: AMB Express

    doi: 10.1186/s13568-020-01042-2

    Identification the binding of pIgG or hIgG to IBPs of S. suis 2 by Far-western blot. SDS-PAGE ( a , c ) and Far-western blot analysis ( b , d ) of the S. suis 2 recombinant IBPs. Recombinant proteins (Enolase, LysM, Pyk, LDH, FBA, KAR) and casein were subjected to SDS-PAGE, then transferred onto PVDF membranes and incubated with pIgG or hIgG. Bound pIgG or hIgG was recognized with HRP conjugated SPA. Casein was used as a negative control for non-specific binding to IgG
    Figure Legend Snippet: Identification the binding of pIgG or hIgG to IBPs of S. suis 2 by Far-western blot. SDS-PAGE ( a , c ) and Far-western blot analysis ( b , d ) of the S. suis 2 recombinant IBPs. Recombinant proteins (Enolase, LysM, Pyk, LDH, FBA, KAR) and casein were subjected to SDS-PAGE, then transferred onto PVDF membranes and incubated with pIgG or hIgG. Bound pIgG or hIgG was recognized with HRP conjugated SPA. Casein was used as a negative control for non-specific binding to IgG

    Techniques Used: Binding Assay, Far Western Blot, SDS Page, Recombinant, Incubation, Negative Control

    Identification the binding of pIgG or hIgG to IBPs of S. suis 2 by dot blot. Recombinant pIBPs ( a ) and hIBPs ( b ) were spotted onto the methanol-activated PVDF membranes and incubated with pIgG or hIgG. Bound pIgG or hIgG was recognized with HRP conjugated SPA. Casein was used as a negative control for non-specific binding to IgG and the membrane with HRP conjugated SPA alone were used as a blank control
    Figure Legend Snippet: Identification the binding of pIgG or hIgG to IBPs of S. suis 2 by dot blot. Recombinant pIBPs ( a ) and hIBPs ( b ) were spotted onto the methanol-activated PVDF membranes and incubated with pIgG or hIgG. Bound pIgG or hIgG was recognized with HRP conjugated SPA. Casein was used as a negative control for non-specific binding to IgG and the membrane with HRP conjugated SPA alone were used as a blank control

    Techniques Used: Binding Assay, Dot Blot, Recombinant, Incubation, Negative Control

    2-DE profiles and Far-western blot to identify the affinity of pIgG and hIgG interactions with S. suis 2 cell wall and extracellular proteins. The cell wall and extracellular proteins of S. suis 2 were loaded into immobilized pH gradient strips for IEF analysis and by SDS-PAGE in the second dimension. The 2-DE gels were transferred onto PVDF membranes and incubated with pIgG or hIgG. Arrows indicate pIBPs or hIBPs detected with HRP conjugated SPA. a 2-DE gel of S. suis 2 extracellular proteins. b Far-western blot of extracellular proteins incubated with pIgG. c Far-western blot of extracellular proteins incubated with hIgG. d 2-DE gel of S. suis 2 cell wall proteins. e Far-western blot of cell wall proteins incubated with pIgG. f Far-western blot of cell wall proteins incubated with hIgG
    Figure Legend Snippet: 2-DE profiles and Far-western blot to identify the affinity of pIgG and hIgG interactions with S. suis 2 cell wall and extracellular proteins. The cell wall and extracellular proteins of S. suis 2 were loaded into immobilized pH gradient strips for IEF analysis and by SDS-PAGE in the second dimension. The 2-DE gels were transferred onto PVDF membranes and incubated with pIgG or hIgG. Arrows indicate pIBPs or hIBPs detected with HRP conjugated SPA. a 2-DE gel of S. suis 2 extracellular proteins. b Far-western blot of extracellular proteins incubated with pIgG. c Far-western blot of extracellular proteins incubated with hIgG. d 2-DE gel of S. suis 2 cell wall proteins. e Far-western blot of cell wall proteins incubated with pIgG. f Far-western blot of cell wall proteins incubated with hIgG

    Techniques Used: Far Western Blot, Electrofocusing, SDS Page, Incubation

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    Boster Bio hrp conjugated staphylococcal protein a spa
    Identification the binding of pIgG or hIgG to IBPs of S. suis 2 by Far-western blot. SDS-PAGE ( a , c ) and Far-western blot analysis ( b , d ) of the S. suis 2 recombinant IBPs. Recombinant proteins (Enolase, LysM, Pyk, LDH, FBA, KAR) and casein were subjected to SDS-PAGE, then transferred onto PVDF membranes and incubated with pIgG or hIgG. Bound pIgG or hIgG was recognized with <t>HRP</t> conjugated <t>SPA.</t> Casein was used as a negative control for non-specific binding to IgG
    Hrp Conjugated Staphylococcal Protein A Spa, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated staphylococcal protein a spa/product/Boster Bio
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated staphylococcal protein a spa - by Bioz Stars, 2022-09
    92/100 stars
      Buy from Supplier

    92
    Boster Bio hrp conjugated spa
    Identification the binding of pIgG or hIgG to IBPs of S. suis 2 by Far-western blot. SDS-PAGE ( a , c ) and Far-western blot analysis ( b , d ) of the S. suis 2 recombinant IBPs. Recombinant proteins (Enolase, LysM, Pyk, LDH, FBA, KAR) and casein were subjected to SDS-PAGE, then transferred onto PVDF membranes and incubated with pIgG or hIgG. Bound pIgG or hIgG was recognized with <t>HRP</t> conjugated <t>SPA.</t> Casein was used as a negative control for non-specific binding to IgG
    Hrp Conjugated Spa, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated spa/product/Boster Bio
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated spa - by Bioz Stars, 2022-09
    92/100 stars
      Buy from Supplier

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    Identification the binding of pIgG or hIgG to IBPs of S. suis 2 by Far-western blot. SDS-PAGE ( a , c ) and Far-western blot analysis ( b , d ) of the S. suis 2 recombinant IBPs. Recombinant proteins (Enolase, LysM, Pyk, LDH, FBA, KAR) and casein were subjected to SDS-PAGE, then transferred onto PVDF membranes and incubated with pIgG or hIgG. Bound pIgG or hIgG was recognized with HRP conjugated SPA. Casein was used as a negative control for non-specific binding to IgG

    Journal: AMB Express

    Article Title: Identification of novel pig and human immunoglobulin G-binding proteins and characterization of the binding regions of enolase from Streptococcus suis serotype 2

    doi: 10.1186/s13568-020-01042-2

    Figure Lengend Snippet: Identification the binding of pIgG or hIgG to IBPs of S. suis 2 by Far-western blot. SDS-PAGE ( a , c ) and Far-western blot analysis ( b , d ) of the S. suis 2 recombinant IBPs. Recombinant proteins (Enolase, LysM, Pyk, LDH, FBA, KAR) and casein were subjected to SDS-PAGE, then transferred onto PVDF membranes and incubated with pIgG or hIgG. Bound pIgG or hIgG was recognized with HRP conjugated SPA. Casein was used as a negative control for non-specific binding to IgG

    Article Snippet: At the same time, a membrane incubated with 20 µg/ml BSA was used as a negative control, followed by three washes with the washing buffer TBST and incubated with HRP conjugated staphylococcal protein A (SPA) (Boster; 1:3000 dilution) for 1 h at 37 °C.

    Techniques: Binding Assay, Far Western Blot, SDS Page, Recombinant, Incubation, Negative Control

    Identification the binding of pIgG or hIgG to IBPs of S. suis 2 by dot blot. Recombinant pIBPs ( a ) and hIBPs ( b ) were spotted onto the methanol-activated PVDF membranes and incubated with pIgG or hIgG. Bound pIgG or hIgG was recognized with HRP conjugated SPA. Casein was used as a negative control for non-specific binding to IgG and the membrane with HRP conjugated SPA alone were used as a blank control

    Journal: AMB Express

    Article Title: Identification of novel pig and human immunoglobulin G-binding proteins and characterization of the binding regions of enolase from Streptococcus suis serotype 2

    doi: 10.1186/s13568-020-01042-2

    Figure Lengend Snippet: Identification the binding of pIgG or hIgG to IBPs of S. suis 2 by dot blot. Recombinant pIBPs ( a ) and hIBPs ( b ) were spotted onto the methanol-activated PVDF membranes and incubated with pIgG or hIgG. Bound pIgG or hIgG was recognized with HRP conjugated SPA. Casein was used as a negative control for non-specific binding to IgG and the membrane with HRP conjugated SPA alone were used as a blank control

    Article Snippet: At the same time, a membrane incubated with 20 µg/ml BSA was used as a negative control, followed by three washes with the washing buffer TBST and incubated with HRP conjugated staphylococcal protein A (SPA) (Boster; 1:3000 dilution) for 1 h at 37 °C.

    Techniques: Binding Assay, Dot Blot, Recombinant, Incubation, Negative Control

    2-DE profiles and Far-western blot to identify the affinity of pIgG and hIgG interactions with S. suis 2 cell wall and extracellular proteins. The cell wall and extracellular proteins of S. suis 2 were loaded into immobilized pH gradient strips for IEF analysis and by SDS-PAGE in the second dimension. The 2-DE gels were transferred onto PVDF membranes and incubated with pIgG or hIgG. Arrows indicate pIBPs or hIBPs detected with HRP conjugated SPA. a 2-DE gel of S. suis 2 extracellular proteins. b Far-western blot of extracellular proteins incubated with pIgG. c Far-western blot of extracellular proteins incubated with hIgG. d 2-DE gel of S. suis 2 cell wall proteins. e Far-western blot of cell wall proteins incubated with pIgG. f Far-western blot of cell wall proteins incubated with hIgG

    Journal: AMB Express

    Article Title: Identification of novel pig and human immunoglobulin G-binding proteins and characterization of the binding regions of enolase from Streptococcus suis serotype 2

    doi: 10.1186/s13568-020-01042-2

    Figure Lengend Snippet: 2-DE profiles and Far-western blot to identify the affinity of pIgG and hIgG interactions with S. suis 2 cell wall and extracellular proteins. The cell wall and extracellular proteins of S. suis 2 were loaded into immobilized pH gradient strips for IEF analysis and by SDS-PAGE in the second dimension. The 2-DE gels were transferred onto PVDF membranes and incubated with pIgG or hIgG. Arrows indicate pIBPs or hIBPs detected with HRP conjugated SPA. a 2-DE gel of S. suis 2 extracellular proteins. b Far-western blot of extracellular proteins incubated with pIgG. c Far-western blot of extracellular proteins incubated with hIgG. d 2-DE gel of S. suis 2 cell wall proteins. e Far-western blot of cell wall proteins incubated with pIgG. f Far-western blot of cell wall proteins incubated with hIgG

    Article Snippet: At the same time, a membrane incubated with 20 µg/ml BSA was used as a negative control, followed by three washes with the washing buffer TBST and incubated with HRP conjugated staphylococcal protein A (SPA) (Boster; 1:3000 dilution) for 1 h at 37 °C.

    Techniques: Far Western Blot, Electrofocusing, SDS Page, Incubation

    Identification the binding of pIgG or hIgG to IBPs of S. suis 2 by Far-western blot. SDS-PAGE ( a , c ) and Far-western blot analysis ( b , d ) of the S. suis 2 recombinant IBPs. Recombinant proteins (Enolase, LysM, Pyk, LDH, FBA, KAR) and casein were subjected to SDS-PAGE, then transferred onto PVDF membranes and incubated with pIgG or hIgG. Bound pIgG or hIgG was recognized with HRP conjugated SPA. Casein was used as a negative control for non-specific binding to IgG

    Journal: AMB Express

    Article Title: Identification of novel pig and human immunoglobulin G-binding proteins and characterization of the binding regions of enolase from Streptococcus suis serotype 2

    doi: 10.1186/s13568-020-01042-2

    Figure Lengend Snippet: Identification the binding of pIgG or hIgG to IBPs of S. suis 2 by Far-western blot. SDS-PAGE ( a , c ) and Far-western blot analysis ( b , d ) of the S. suis 2 recombinant IBPs. Recombinant proteins (Enolase, LysM, Pyk, LDH, FBA, KAR) and casein were subjected to SDS-PAGE, then transferred onto PVDF membranes and incubated with pIgG or hIgG. Bound pIgG or hIgG was recognized with HRP conjugated SPA. Casein was used as a negative control for non-specific binding to IgG

    Article Snippet: Subsequently, the membranes were washed three times with the washing buffer TBST and incubated with HRP conjugated SPA (Boster; 0.2 µg/ml) for 1 h at 37 °C.

    Techniques: Binding Assay, Far Western Blot, SDS Page, Recombinant, Incubation, Negative Control

    Identification the binding of pIgG or hIgG to IBPs of S. suis 2 by dot blot. Recombinant pIBPs ( a ) and hIBPs ( b ) were spotted onto the methanol-activated PVDF membranes and incubated with pIgG or hIgG. Bound pIgG or hIgG was recognized with HRP conjugated SPA. Casein was used as a negative control for non-specific binding to IgG and the membrane with HRP conjugated SPA alone were used as a blank control

    Journal: AMB Express

    Article Title: Identification of novel pig and human immunoglobulin G-binding proteins and characterization of the binding regions of enolase from Streptococcus suis serotype 2

    doi: 10.1186/s13568-020-01042-2

    Figure Lengend Snippet: Identification the binding of pIgG or hIgG to IBPs of S. suis 2 by dot blot. Recombinant pIBPs ( a ) and hIBPs ( b ) were spotted onto the methanol-activated PVDF membranes and incubated with pIgG or hIgG. Bound pIgG or hIgG was recognized with HRP conjugated SPA. Casein was used as a negative control for non-specific binding to IgG and the membrane with HRP conjugated SPA alone were used as a blank control

    Article Snippet: Subsequently, the membranes were washed three times with the washing buffer TBST and incubated with HRP conjugated SPA (Boster; 0.2 µg/ml) for 1 h at 37 °C.

    Techniques: Binding Assay, Dot Blot, Recombinant, Incubation, Negative Control

    2-DE profiles and Far-western blot to identify the affinity of pIgG and hIgG interactions with S. suis 2 cell wall and extracellular proteins. The cell wall and extracellular proteins of S. suis 2 were loaded into immobilized pH gradient strips for IEF analysis and by SDS-PAGE in the second dimension. The 2-DE gels were transferred onto PVDF membranes and incubated with pIgG or hIgG. Arrows indicate pIBPs or hIBPs detected with HRP conjugated SPA. a 2-DE gel of S. suis 2 extracellular proteins. b Far-western blot of extracellular proteins incubated with pIgG. c Far-western blot of extracellular proteins incubated with hIgG. d 2-DE gel of S. suis 2 cell wall proteins. e Far-western blot of cell wall proteins incubated with pIgG. f Far-western blot of cell wall proteins incubated with hIgG

    Journal: AMB Express

    Article Title: Identification of novel pig and human immunoglobulin G-binding proteins and characterization of the binding regions of enolase from Streptococcus suis serotype 2

    doi: 10.1186/s13568-020-01042-2

    Figure Lengend Snippet: 2-DE profiles and Far-western blot to identify the affinity of pIgG and hIgG interactions with S. suis 2 cell wall and extracellular proteins. The cell wall and extracellular proteins of S. suis 2 were loaded into immobilized pH gradient strips for IEF analysis and by SDS-PAGE in the second dimension. The 2-DE gels were transferred onto PVDF membranes and incubated with pIgG or hIgG. Arrows indicate pIBPs or hIBPs detected with HRP conjugated SPA. a 2-DE gel of S. suis 2 extracellular proteins. b Far-western blot of extracellular proteins incubated with pIgG. c Far-western blot of extracellular proteins incubated with hIgG. d 2-DE gel of S. suis 2 cell wall proteins. e Far-western blot of cell wall proteins incubated with pIgG. f Far-western blot of cell wall proteins incubated with hIgG

    Article Snippet: Subsequently, the membranes were washed three times with the washing buffer TBST and incubated with HRP conjugated SPA (Boster; 0.2 µg/ml) for 1 h at 37 °C.

    Techniques: Far Western Blot, Electrofocusing, SDS Page, Incubation