Structured Review

WuXi AppTec hrp conjugated secondary antibodies
Hrp Conjugated Secondary Antibodies, supplied by WuXi AppTec, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hrp conjugated secondary antibodies/product/WuXi AppTec
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
hrp conjugated secondary antibodies - by Bioz Stars, 2020-07
92/100 stars

Images

Related Articles

Western Blot:

Article Title: MiR-200a inhibits epithelial-mesenchymal transition of pancreatic cancer stem cell
Article Snippet: .. HRP-conjugated secondary antibodies (HRP Goat anti-Rabbit IgG Antibody, 1: 5000, Abgent; HRP Goat anti-Mouse IgG Antibody, 1: 5000, Abgent) were incubated at room temperature (RT) for 1 h and detected using the enhanced chemiluminesence detection system.Total protein was extracted from untreated and the cells treated by transfecting miR-200a and subjected to western blot analysis as described to evaluate the expression of E-cadherin, N-cadherin, ZEB1 and vimentin. ..

Incubation:

Article Title: MiR-200a inhibits epithelial-mesenchymal transition of pancreatic cancer stem cell
Article Snippet: .. HRP-conjugated secondary antibodies (HRP Goat anti-Rabbit IgG Antibody, 1: 5000, Abgent; HRP Goat anti-Mouse IgG Antibody, 1: 5000, Abgent) were incubated at room temperature (RT) for 1 h and detected using the enhanced chemiluminesence detection system.Total protein was extracted from untreated and the cells treated by transfecting miR-200a and subjected to western blot analysis as described to evaluate the expression of E-cadherin, N-cadherin, ZEB1 and vimentin. ..

Article Title: Necrosulfonamide Ameliorates Neurological Impairment in Spinal Cord Injury by Improving Antioxidative Capacity
Article Snippet: .. They were then incubated with HRP-conjugated secondary antibodies (Abgent, 1:30,000), which was followed by chemiluminescent substrate development (Bio-Rad). .. Neun and MLKL Double Immunofluorescence Staining Cells were fixed with 4% paraformaldehyde for 15 min at 37°C, washed three times with PBS, and then, incubated overnight at 4°C in PBS containing 2% Goat serum, 0.03% Triton X-100 in, p-MLKL antibody (CST, #37333, 1:1,200), and NeuN antibody (Abcam, ab177487, 1:500).

Expressing:

Article Title: MiR-200a inhibits epithelial-mesenchymal transition of pancreatic cancer stem cell
Article Snippet: .. HRP-conjugated secondary antibodies (HRP Goat anti-Rabbit IgG Antibody, 1: 5000, Abgent; HRP Goat anti-Mouse IgG Antibody, 1: 5000, Abgent) were incubated at room temperature (RT) for 1 h and detected using the enhanced chemiluminesence detection system.Total protein was extracted from untreated and the cells treated by transfecting miR-200a and subjected to western blot analysis as described to evaluate the expression of E-cadherin, N-cadherin, ZEB1 and vimentin. ..

FLAG-tag:

Article Title: UBIAD1 suppresses the proliferation of bladder carcinoma cells by regulating H-Ras intracellular trafficking via interaction with the C-terminal domain of H-Ras
Article Snippet: .. Antibodies The following antibodies were used in this study: p-c-Raf (Ser259) (#9421), p-c-Raf (Ser338) (#9427), p-c-Raf (Ser289/296/301) (#9431), c-Raf (#9422), p-ERK1/2 (#4370), total-ERK1/2 (#4695), and caspase-3 (#9662) from Cell Signaling Technology (USA); anti-phospho-MEK (Ser218/222)/MEK2 (Ser222/226) (#2283379) from Millipore (USA); MEK1/2 (Ab-217/221) (#21203) antibody from Signalway Antibody (USA); Antibodies detecting actin (A01010-1) and GAPDH (A01020-1) from Abbkine (USA); Flag tag monoclonal antibody (A00187) from GenScript (China); GFP tag antibody (#66002-1) and HMGB1 (#10829-1) from proteintech (China); 488/549/645-conjugated secondary antibody from Abbkine (USA); HRP-conjugated secondary antibody from ABGENT (USA); UBIAD1 antibody (ab36832), Pan-Ras antibody (ab16907), and LC3B (AB192890) from Abcam (USA); rabbit IgG , mouse IgG from Immunoreagents (USA). .. Antibodies used for experiments with Drosophila were as follows: p-ERK (sc-7976) and ERK2 (sc-153) from Santa Cruz (US), pan-Ras (#3339) from Cell Signaling Technology (USA).

Article Title: UBIAD1 suppresses the proliferation of bladder carcinoma cells by regulating H-Ras intracellular trafficking via interaction with the C-terminal domain of H-Ras
Article Snippet: .. The following antibodies were used in this study: p-c-Raf (Ser259) (#9421), p-c-Raf (Ser338) (#9427), p-c-Raf (Ser289/296/301) (#9431), c-Raf (#9422), p-ERK1/2 (#4370), total-ERK1/2 (#4695), and caspase-3 (#9662) from Cell Signaling Technology (USA); anti-phospho-MEK (Ser218/222)/MEK2 (Ser222/226) (#2283379) from Millipore (USA); MEK1/2 (Ab-217/221) (#21203) antibody from Signalway Antibody (USA); Antibodies detecting actin (A01010-1) and GAPDH (A01020-1) from Abbkine (USA); Flag tag monoclonal antibody (A00187) from GenScript (China); GFP tag antibody (#66002-1) and HMGB1 (#10829-1) from proteintech (China); 488/549/645-conjugated secondary antibody from Abbkine (USA); HRP-conjugated secondary antibody from ABGENT (USA); UBIAD1 antibody (ab36832), Pan-Ras antibody (ab16907), and LC3B (AB192890) from Abcam (USA); rabbit IgG, mouse IgG from Immunoreagents (USA). .. Antibodies used for experiments with Drosophila were as follows: p-ERK (sc-7976) and ERK2 (sc-153) from Santa Cruz (US), pan-Ras (#3339) from Cell Signaling Technology (USA).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91
    WuXi AppTec pias3
    The tumor suppressor genes PTEN, PDCD4 and <t>PIAS3,</t> targeted by miR-21, are downregulated after BCR stimulation. ( a,b ) Ramos stable transfectants were stimulated with 5 µg/mL F(ab’) 2 anti-IgM for 5 minutes and 48 hours. Enhanced STAT3 and ERK1/2 phosphorylation was observed in IgM-BCR stimulated cells for 5 minutes. PTEN, PIAS3 and PDCD4 downregulation was observed in IgM-BCR stimulated cells after 48 hours. ( c,d ) Gene set enrichment analysis (GSEA) for Gabriely_miR-21_targets gene set (N = 289). The panel shows GSEA of Ramos GFP-ZAP-70 stimulated with 5 µg/mL F(ab’) 2 anti-IgM for 4 hours versus Ramos GPF-ZAP-70 unstimulated ( c ). The enrichment plot shows GSEA of Ramos GFP-ZAP-70 versus Ramos GFP cells, both stimulated with 5 µg/mL F(ab’) 2 anti-IgM for 4 hours ( d ). ES: enrichment score.
    Pias3, supplied by WuXi AppTec, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pias3/product/WuXi AppTec
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    pias3 - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    93
    WuXi AppTec vegf
    The tumor suppressor genes PTEN, PDCD4 and <t>PIAS3,</t> targeted by miR-21, are downregulated after BCR stimulation. ( a,b ) Ramos stable transfectants were stimulated with 5 µg/mL F(ab’) 2 anti-IgM for 5 minutes and 48 hours. Enhanced STAT3 and ERK1/2 phosphorylation was observed in IgM-BCR stimulated cells for 5 minutes. PTEN, PIAS3 and PDCD4 downregulation was observed in IgM-BCR stimulated cells after 48 hours. ( c,d ) Gene set enrichment analysis (GSEA) for Gabriely_miR-21_targets gene set (N = 289). The panel shows GSEA of Ramos GFP-ZAP-70 stimulated with 5 µg/mL F(ab’) 2 anti-IgM for 4 hours versus Ramos GPF-ZAP-70 unstimulated ( c ). The enrichment plot shows GSEA of Ramos GFP-ZAP-70 versus Ramos GFP cells, both stimulated with 5 µg/mL F(ab’) 2 anti-IgM for 4 hours ( d ). ES: enrichment score.
    Vegf, supplied by WuXi AppTec, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vegf/product/WuXi AppTec
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    vegf - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    Image Search Results


    The tumor suppressor genes PTEN, PDCD4 and PIAS3, targeted by miR-21, are downregulated after BCR stimulation. ( a,b ) Ramos stable transfectants were stimulated with 5 µg/mL F(ab’) 2 anti-IgM for 5 minutes and 48 hours. Enhanced STAT3 and ERK1/2 phosphorylation was observed in IgM-BCR stimulated cells for 5 minutes. PTEN, PIAS3 and PDCD4 downregulation was observed in IgM-BCR stimulated cells after 48 hours. ( c,d ) Gene set enrichment analysis (GSEA) for Gabriely_miR-21_targets gene set (N = 289). The panel shows GSEA of Ramos GFP-ZAP-70 stimulated with 5 µg/mL F(ab’) 2 anti-IgM for 4 hours versus Ramos GPF-ZAP-70 unstimulated ( c ). The enrichment plot shows GSEA of Ramos GFP-ZAP-70 versus Ramos GFP cells, both stimulated with 5 µg/mL F(ab’) 2 anti-IgM for 4 hours ( d ). ES: enrichment score.

    Journal: Scientific Reports

    Article Title: Microenvironment regulates the expression of miR-21 and tumor suppressor genes PTEN, PIAS3 and PDCD4 through ZAP-70 in chronic lymphocytic leukemia

    doi: 10.1038/s41598-017-12135-7

    Figure Lengend Snippet: The tumor suppressor genes PTEN, PDCD4 and PIAS3, targeted by miR-21, are downregulated after BCR stimulation. ( a,b ) Ramos stable transfectants were stimulated with 5 µg/mL F(ab’) 2 anti-IgM for 5 minutes and 48 hours. Enhanced STAT3 and ERK1/2 phosphorylation was observed in IgM-BCR stimulated cells for 5 minutes. PTEN, PIAS3 and PDCD4 downregulation was observed in IgM-BCR stimulated cells after 48 hours. ( c,d ) Gene set enrichment analysis (GSEA) for Gabriely_miR-21_targets gene set (N = 289). The panel shows GSEA of Ramos GFP-ZAP-70 stimulated with 5 µg/mL F(ab’) 2 anti-IgM for 4 hours versus Ramos GPF-ZAP-70 unstimulated ( c ). The enrichment plot shows GSEA of Ramos GFP-ZAP-70 versus Ramos GFP cells, both stimulated with 5 µg/mL F(ab’) 2 anti-IgM for 4 hours ( d ). ES: enrichment score.

    Article Snippet: Membranes were incubated overnight at 4 °C with primary antibodies against phospho-ZAP-70Tyr319 /SykTyr352 , phospho-AktSer473 , phospho-ERK1/2Thr202/Tyr204 , phospho-STAT3Ser727 , Akt, ERK1/2, PTEN and PDCD4 (Cell signalling Technology Inc.), ZAP-70 (clone 2F3.2; Upstate Biotechnology), PIAS3 (WuXi AppTec.ABGENT) and β-actin (Abcam).

    Techniques:

    Co-culture of primary CLL cells with BMSC, CD40L and CpG ODN induces the expression of miR-21 and downregulation of the miR-21 targets PTEN, PDCD4 and PIAS3. ( a ) Primary CLL cells from 40 patients were cultured in suspension or in co-culture for 48 hours and miR-21 expression was measured by QRT-PCR. ( b ) Primary CLL cells from 22 patients were cultured in suspension or in co-culture for 48 hours. PTEN, PDCD4 and PIAS3 expression was determined by QRT-PCR. ( c – d ) Relative gene expression comparison between matched samples from peripheral blood (PB) and lymph nodes (LN) from 17 treatment-naive CLL patients, using public data from Y.Herishanu et al ., Blood 2011 23 . mRNA expression level of miR-21 ( c ) and PTEN ( d ) were analyzed and compared by Wilcoxon matched pairs signed rank test. ( e ) PBMC from 13 patients were pre-treated with 5 µM PCI-32765 for 1 hour and cultured in suspension or in co-culture for 48 hours. Expression levels of miR-21 were measured by QRT-PCR. *P

    Journal: Scientific Reports

    Article Title: Microenvironment regulates the expression of miR-21 and tumor suppressor genes PTEN, PIAS3 and PDCD4 through ZAP-70 in chronic lymphocytic leukemia

    doi: 10.1038/s41598-017-12135-7

    Figure Lengend Snippet: Co-culture of primary CLL cells with BMSC, CD40L and CpG ODN induces the expression of miR-21 and downregulation of the miR-21 targets PTEN, PDCD4 and PIAS3. ( a ) Primary CLL cells from 40 patients were cultured in suspension or in co-culture for 48 hours and miR-21 expression was measured by QRT-PCR. ( b ) Primary CLL cells from 22 patients were cultured in suspension or in co-culture for 48 hours. PTEN, PDCD4 and PIAS3 expression was determined by QRT-PCR. ( c – d ) Relative gene expression comparison between matched samples from peripheral blood (PB) and lymph nodes (LN) from 17 treatment-naive CLL patients, using public data from Y.Herishanu et al ., Blood 2011 23 . mRNA expression level of miR-21 ( c ) and PTEN ( d ) were analyzed and compared by Wilcoxon matched pairs signed rank test. ( e ) PBMC from 13 patients were pre-treated with 5 µM PCI-32765 for 1 hour and cultured in suspension or in co-culture for 48 hours. Expression levels of miR-21 were measured by QRT-PCR. *P

    Article Snippet: Membranes were incubated overnight at 4 °C with primary antibodies against phospho-ZAP-70Tyr319 /SykTyr352 , phospho-AktSer473 , phospho-ERK1/2Thr202/Tyr204 , phospho-STAT3Ser727 , Akt, ERK1/2, PTEN and PDCD4 (Cell signalling Technology Inc.), ZAP-70 (clone 2F3.2; Upstate Biotechnology), PIAS3 (WuXi AppTec.ABGENT) and β-actin (Abcam).

    Techniques: Co-Culture Assay, Expressing, Cell Culture, Quantitative RT-PCR