hrp conjugated igg secondary antibodies  (WuXi AppTec)


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    Structured Review

    WuXi AppTec hrp conjugated igg secondary antibodies
    Hrp Conjugated Igg Secondary Antibodies, supplied by WuXi AppTec, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated igg secondary antibodies/product/WuXi AppTec
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated igg secondary antibodies - by Bioz Stars, 2021-07
    86/100 stars

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    Immunoprecipitation:

    Article Title: Furin Is the Major Processing Enzyme of the Cardiac-specific Growth Factor Bone Morphogenetic Protein 10 *
    Article Snippet: Proteins from medium of cultured cells (transfected with ProtC-tagged pro-BMP10) were analyzed by 8% Tris glycine SDS-PAGE, followed by Western blotting using a ProtC-Ab (1:1000; GenScript) and a rabbit HRP-conjugated secondary Ab (1:10,000; Invitrogen). .. Pro-BMP10 and its prosegment tagged with ProtC at the N terminus were immunoprecipitated from mouse primary hepatocytes culture medium (200 μl) using a ProtC-Ab (2 μg; Abgent), resolved by 8% Tris glycine SDS-PAGE gels, and immunodetected using the same ProtC-Ab (1:1,000) and anti-rabbit IgG-HRP (1:2,000, TrueBlot). .. The antigen-antibody complexes were visualized using the enhanced chemiluminescence kit (ECL; Pierce).

    SDS Page:

    Article Title: Furin Is the Major Processing Enzyme of the Cardiac-specific Growth Factor Bone Morphogenetic Protein 10 *
    Article Snippet: Proteins from medium of cultured cells (transfected with ProtC-tagged pro-BMP10) were analyzed by 8% Tris glycine SDS-PAGE, followed by Western blotting using a ProtC-Ab (1:1000; GenScript) and a rabbit HRP-conjugated secondary Ab (1:10,000; Invitrogen). .. Pro-BMP10 and its prosegment tagged with ProtC at the N terminus were immunoprecipitated from mouse primary hepatocytes culture medium (200 μl) using a ProtC-Ab (2 μg; Abgent), resolved by 8% Tris glycine SDS-PAGE gels, and immunodetected using the same ProtC-Ab (1:1,000) and anti-rabbit IgG-HRP (1:2,000, TrueBlot). .. The antigen-antibody complexes were visualized using the enhanced chemiluminescence kit (ECL; Pierce).

    other:

    Article Title: The Clustered, Regularly Interspaced, Short Palindromic Repeats-associated Endonuclease 9 (CRISPR/Cas9)-created MDM2 T309G Mutation Enhances Vitreous-induced Expression of MDM2 and Proliferation and Survival of Cells *
    Article Snippet: The antibody against MDM2 was from Abgent (San Diego, CA).

    Article Title: Elevated p62/SQSTM1 determines the fate of autophagy-deficient neural stem cells by increasing superoxide
    Article Snippet: Antibodies and reagentsPrimary antibodies used were: mouse anti-BrdU (G3G4; DSHB), mouse anti-GFAP (Thermo Fisher Scientific), rhodamine-conjugated mouse anti-BrdU (Millipore), mouse anti-nestin (Rat-401), mouse anti-NeuN (Millipore), mouse anti–Nqo-1 (Santa Cruz Biotechnology), mouse anti-p62 (Abcam), mouse anti ubiquitin (Santa Cruz Biotechnology), mouse antivinculin (Sigma-Aldrich), rabbit anti-Atg5 (Novus Biologicals), rabbit anti-Atg16L1 (Abgent), rabbit anti-GFAP (Dako), rabbit anti-Ki67 (Spring Bioscience), rabbit anti-LC3 (Cell Signaling), rabbit anti-NBR1 (Cell Signaling), rabbit anti-Nrf2 (Abcam), rabbit anti-p62 (Enzo), rabbit anti-Sox2 (Millipore), rabbit anti-TAX1BP1 (Abgent), rabbit anti-Fip200 (ProteinTech), rabbit anti-olig2 (Millipore), rat anti-Ki67 (BioLegend), and guinea pig antidoublecortin (anti-DCX; EMD Millipore).

    Incubation:

    Article Title: Antisense directed against PS-1 gene decreases brain oxidative markers in aged senescence accelerated mice (SAMP8) and reverses learning and memory impairment: a proteomics study
    Article Snippet: Following 1D- or 2D-PAGE, in-gel proteins were transferred using a Trans-Blot Semi-Dry Transfer Cell system at 20 V for 2 h (Bio-Rad, Hercules, CA) into a nitrocellulose membrane (0.45 μm). .. Post-transfer, membranes were incubated in a blocking solution of 3% BSA in Wash Blot at RT for 2 h. Following blocking, membranes were incubated with rabbit polyclonal antibody anti-Pin-1 (1:2000) (Santa Cruz Biotechnology, Santa Cruz, CA), rabbit polyclonal antibody anti-isoform M1 pyruvate kinase isozymes M1/M2 (KPYM) (1:500) (ABGENT, USA), and rabbit polyclonal antibody anti-actin (Sigma-Aldrich ) at RT on a rocking platform for 2–3 h. Blots were washed three times for 5 min each in wash blot, followed by 1 h incubation with rabbit IgG alkaline phosphatase (Sigma-Aldrich) (1:5000) secondary antibody and Amersham ECL rabbit IgG HRP-linked (1:5000) secondary antibody at RT. .. Blots were rinsed three times for 5 min each in wash blot and developed colorimetrically using BCIP/NBT in ALP buffer and chemifluorescently (λex/λem: 70/618 nm), respectively.

    Blocking Assay:

    Article Title: Antisense directed against PS-1 gene decreases brain oxidative markers in aged senescence accelerated mice (SAMP8) and reverses learning and memory impairment: a proteomics study
    Article Snippet: Following 1D- or 2D-PAGE, in-gel proteins were transferred using a Trans-Blot Semi-Dry Transfer Cell system at 20 V for 2 h (Bio-Rad, Hercules, CA) into a nitrocellulose membrane (0.45 μm). .. Post-transfer, membranes were incubated in a blocking solution of 3% BSA in Wash Blot at RT for 2 h. Following blocking, membranes were incubated with rabbit polyclonal antibody anti-Pin-1 (1:2000) (Santa Cruz Biotechnology, Santa Cruz, CA), rabbit polyclonal antibody anti-isoform M1 pyruvate kinase isozymes M1/M2 (KPYM) (1:500) (ABGENT, USA), and rabbit polyclonal antibody anti-actin (Sigma-Aldrich ) at RT on a rocking platform for 2–3 h. Blots were washed three times for 5 min each in wash blot, followed by 1 h incubation with rabbit IgG alkaline phosphatase (Sigma-Aldrich) (1:5000) secondary antibody and Amersham ECL rabbit IgG HRP-linked (1:5000) secondary antibody at RT. .. Blots were rinsed three times for 5 min each in wash blot and developed colorimetrically using BCIP/NBT in ALP buffer and chemifluorescently (λex/λem: 70/618 nm), respectively.

    Western Blot:

    Article Title: ABCB5 identifies a therapy-refractory tumor cell population in colorectal cancer patients
    Article Snippet: Unconjugated MOPC-31C mouse isotype control mAb was purchased from BD Pharmingen (San Diego, CA) and APC-conjugated secondary Ab from eBioscience (San Diego, CA). .. For Western blots the following polyclonal Abs (pAbs) were used: rabbit anti-human ABCB5 (Abgent, San Diego, CA), rabbit anti-human α-Tubulin (Abcam, Cambridge, MA), and HRP-conjugated donkey anti-rabbit IgG (Jackson Immunoresearch, West Grove, PA). .. The following Abs were used for immunohistochemistry or immunofluorescence staining: Rabbit anti-CD133 (Abcam, Cambridge, MA), Alkaline phosphatase horse anti-mouse or goat anti-rabbit IgG, peroxidase goat anti-rabbit IgG, Alexa Fluor 594 donkey anti-rabbit IgG and Alexa Fluor 488 donkey anti-mouse IgG (Invitrogen, Carisbad, CA).

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    WuXi AppTec antibody conjugated to hrp
    Antibody Conjugated To Hrp, supplied by WuXi AppTec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibody conjugated to hrp/product/WuXi AppTec
    Average 93 stars, based on 1 article reviews
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    86
    WuXi AppTec hrp conjugated igg secondary antibodies
    Hrp Conjugated Igg Secondary Antibodies, supplied by WuXi AppTec, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated igg secondary antibodies/product/WuXi AppTec
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated igg secondary antibodies - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

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    WuXi AppTec horse radish peroxidase hrp conjugated goat anti rabbit mouse secondary antibodies
    Horse Radish Peroxidase Hrp Conjugated Goat Anti Rabbit Mouse Secondary Antibodies, supplied by WuXi AppTec, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horse radish peroxidase hrp conjugated goat anti rabbit mouse secondary antibodies/product/WuXi AppTec
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    horse radish peroxidase hrp conjugated goat anti rabbit mouse secondary antibodies - by Bioz Stars, 2021-07
    86/100 stars
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