Structured Review

The Jackson Laboratory hrp conjugated secondary antibodies
Integrin β5 protein expression is increased on TGFβ-induced Mfs. HCFs were treated with TGFβ1 or FGF-2 plus heparin for 72 hours and analyzed for β5 and β3 cell-surface expression. (A) Flow cytometry (B) Cell-surface biotinylation; lysates were immunoprecipitated (IP) with antibody against integrin β5, separated by SDS-PAGE and probed with <t>streptavidin-HRP</t> to detect biotinylated proteins. Bands were identified by molecular weight. GAPDH indicates equal protein input for IP. Quantification of the β5 band relative to GAPDH input control is shown. (C) Real-time PCR for β5, β3, and uPAR at Day 1 and Day 7 after TGFβ1 treatment. *p value
Hrp Conjugated Secondary Antibodies, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 93/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hrp conjugated secondary antibodies/product/The Jackson Laboratory
Average 93 stars, based on 43 article reviews
Price from $9.99 to $1999.99
hrp conjugated secondary antibodies - by Bioz Stars, 2020-07
93/100 stars

Images

1) Product Images from "Degradation of Internalized ?v?5 Integrin Is Controlled by uPAR Bound uPA: Effect on ?1 Integrin Activity and ?-SMA Stress Fiber Assembly"

Article Title: Degradation of Internalized ?v?5 Integrin Is Controlled by uPAR Bound uPA: Effect on ?1 Integrin Activity and ?-SMA Stress Fiber Assembly

Journal: PLoS ONE

doi: 10.1371/journal.pone.0033915

Integrin β5 protein expression is increased on TGFβ-induced Mfs. HCFs were treated with TGFβ1 or FGF-2 plus heparin for 72 hours and analyzed for β5 and β3 cell-surface expression. (A) Flow cytometry (B) Cell-surface biotinylation; lysates were immunoprecipitated (IP) with antibody against integrin β5, separated by SDS-PAGE and probed with streptavidin-HRP to detect biotinylated proteins. Bands were identified by molecular weight. GAPDH indicates equal protein input for IP. Quantification of the β5 band relative to GAPDH input control is shown. (C) Real-time PCR for β5, β3, and uPAR at Day 1 and Day 7 after TGFβ1 treatment. *p value
Figure Legend Snippet: Integrin β5 protein expression is increased on TGFβ-induced Mfs. HCFs were treated with TGFβ1 or FGF-2 plus heparin for 72 hours and analyzed for β5 and β3 cell-surface expression. (A) Flow cytometry (B) Cell-surface biotinylation; lysates were immunoprecipitated (IP) with antibody against integrin β5, separated by SDS-PAGE and probed with streptavidin-HRP to detect biotinylated proteins. Bands were identified by molecular weight. GAPDH indicates equal protein input for IP. Quantification of the β5 band relative to GAPDH input control is shown. (C) Real-time PCR for β5, β3, and uPAR at Day 1 and Day 7 after TGFβ1 treatment. *p value

Techniques Used: Expressing, Flow Cytometry, Cytometry, Immunoprecipitation, SDS Page, Molecular Weight, Real-time Polymerase Chain Reaction

2) Product Images from "Plasminogen Activator Inhibitor-1 Regulates Integrin ?v?3 Expression and Autocrine Transforming Growth Factor ? Signaling *"

Article Title: Plasminogen Activator Inhibitor-1 Regulates Integrin ?v?3 Expression and Autocrine Transforming Growth Factor ? Signaling *

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M109.018804

β3 and TGFβRII expression is increased in PAI-1 KO cells. A , cell surface expression. WT and KO cells were biotinylated on the cell surface before lysis and immunoprecipitated for β3 or TGFβRII. Western blots were probed with streptavidin-HRP ( SA-HRP ) to detect biotinylated proteins. Bands were identified by molecular weight. B , total expression. WT and KO cell lysates were Western blotted for β3 or TGFβRII. Vimentin controls confirm equal loading (densitometry).
Figure Legend Snippet: β3 and TGFβRII expression is increased in PAI-1 KO cells. A , cell surface expression. WT and KO cells were biotinylated on the cell surface before lysis and immunoprecipitated for β3 or TGFβRII. Western blots were probed with streptavidin-HRP ( SA-HRP ) to detect biotinylated proteins. Bands were identified by molecular weight. B , total expression. WT and KO cell lysates were Western blotted for β3 or TGFβRII. Vimentin controls confirm equal loading (densitometry).

Techniques Used: Expressing, Lysis, Immunoprecipitation, Western Blot, Molecular Weight

Related Articles

Incubation:

Article Title: Indole-3-Carbinol Induces Apoptosis in Human Osteosarcoma MG-63 and U2OS Cells
Article Snippet: .. The membranes were then washed with TBS-T, and incubated using HRP-conjugated secondary antibody (Jackson Laboratory, Bar Harbor, USA). .. Chemiluminescence was detected using ECL (Gendepot, Barker, USA) and measured using the Chemi-doc detection system (Bio-Rad, Hercules, CA, USA)

Article Title: Long noncoding RNA H19 derived miR-675 regulates cell proliferation by down-regulating E2F-1 in human pancreatic ductal adenocarcinoma
Article Snippet: .. The membranes were then incubated with HRP-conjugated secondary antibody (Jackson Lab, USA) at 4℃ for 2 h. The protein bands were detected using chemiluminescence (Millipore, USA) and exposed to X-ray films. .. Xenograft study Wild type AsPC-1 (2×106 ) and PANC-1 cells (1×107 ) were subcutaneously injected into the axillary fossa of five-week-old male Balb/c (nu/nu) nude mice.

other:

Article Title: Inhibition of neural crest formation by Kctd15 involves regulation of transcription factor AP-2
Article Snippet: HRP-conjugated secondary antibodies were from Jackson Laboratories.

Article Title: ZYZ-803 Mitigates Endoplasmic Reticulum Stress-Related Necroptosis after Acute Myocardial Infarction through Downregulating the RIP3-CaMKII Signaling Pathway
Article Snippet: HRP-conjugated secondary antibodies were from Jackson Laboratories (West Grove, PA, USA).

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    The Jackson Laboratory goat anti mouse hrp conjugated secondary antibodies
    An N-glycosylation analysis of the fE1E2 and tE1E2 complex expressed in L. tarentolae . ( A ) The recombinant complexes were treated with endoglycosidase PNGaseF. After overnight incubation at 37 °C in native conditions, western blot under reducing conditions with <t>anti-E2</t> Ab was performed. The E1E2 complex expressed in HEK 293 cells was used as the control (hE1E2). ( B ) Dose-dependent binding of the E1E2 complexes to GNA lectin. Decreasing concentrations of cell lysates (5-fold dilutions - from 1:5 to 1:3125) were captured in ELISA plates coated with GNA lectin. The bound antigens were visualized with anti-E2 Abs, anti-mouse IgG <t>HRP</t> conjugate, and the TMB substrate. The error bars represent the standard deviations of 2 replicate values.
    Goat Anti Mouse Hrp Conjugated Secondary Antibodies, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse hrp conjugated secondary antibodies/product/The Jackson Laboratory
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse hrp conjugated secondary antibodies - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    93
    The Jackson Laboratory hrp conjugated secondary antibodies
    Integrin β5 protein expression is increased on TGFβ-induced Mfs. HCFs were treated with TGFβ1 or FGF-2 plus heparin for 72 hours and analyzed for β5 and β3 cell-surface expression. (A) Flow cytometry (B) Cell-surface biotinylation; lysates were immunoprecipitated (IP) with antibody against integrin β5, separated by SDS-PAGE and probed with <t>streptavidin-HRP</t> to detect biotinylated proteins. Bands were identified by molecular weight. GAPDH indicates equal protein input for IP. Quantification of the β5 band relative to GAPDH input control is shown. (C) Real-time PCR for β5, β3, and uPAR at Day 1 and Day 7 after TGFβ1 treatment. *p value
    Hrp Conjugated Secondary Antibodies, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 93/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated secondary antibodies/product/The Jackson Laboratory
    Average 93 stars, based on 43 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated secondary antibodies - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    92
    The Jackson Laboratory anti mouse igg
    Integrin β5 protein expression is increased on TGFβ-induced Mfs. HCFs were treated with TGFβ1 or FGF-2 plus heparin for 72 hours and analyzed for β5 and β3 cell-surface expression. (A) Flow cytometry (B) Cell-surface biotinylation; lysates were immunoprecipitated (IP) with antibody against integrin β5, separated by SDS-PAGE and probed with <t>streptavidin-HRP</t> to detect biotinylated proteins. Bands were identified by molecular weight. GAPDH indicates equal protein input for IP. Quantification of the β5 band relative to GAPDH input control is shown. (C) Real-time PCR for β5, β3, and uPAR at Day 1 and Day 7 after TGFβ1 treatment. *p value
    Anti Mouse Igg, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mouse igg/product/The Jackson Laboratory
    Average 92 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    anti mouse igg - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    Image Search Results


    An N-glycosylation analysis of the fE1E2 and tE1E2 complex expressed in L. tarentolae . ( A ) The recombinant complexes were treated with endoglycosidase PNGaseF. After overnight incubation at 37 °C in native conditions, western blot under reducing conditions with anti-E2 Ab was performed. The E1E2 complex expressed in HEK 293 cells was used as the control (hE1E2). ( B ) Dose-dependent binding of the E1E2 complexes to GNA lectin. Decreasing concentrations of cell lysates (5-fold dilutions - from 1:5 to 1:3125) were captured in ELISA plates coated with GNA lectin. The bound antigens were visualized with anti-E2 Abs, anti-mouse IgG HRP conjugate, and the TMB substrate. The error bars represent the standard deviations of 2 replicate values.

    Journal: Scientific Reports

    Article Title: Immunogenicity and functional characterization of Leishmania-derived hepatitis C virus envelope glycoprotein complex

    doi: 10.1038/srep30627

    Figure Lengend Snippet: An N-glycosylation analysis of the fE1E2 and tE1E2 complex expressed in L. tarentolae . ( A ) The recombinant complexes were treated with endoglycosidase PNGaseF. After overnight incubation at 37 °C in native conditions, western blot under reducing conditions with anti-E2 Ab was performed. The E1E2 complex expressed in HEK 293 cells was used as the control (hE1E2). ( B ) Dose-dependent binding of the E1E2 complexes to GNA lectin. Decreasing concentrations of cell lysates (5-fold dilutions - from 1:5 to 1:3125) were captured in ELISA plates coated with GNA lectin. The bound antigens were visualized with anti-E2 Abs, anti-mouse IgG HRP conjugate, and the TMB substrate. The error bars represent the standard deviations of 2 replicate values.

    Article Snippet: The binding to the lectin was determined using anti-E2 AP33 (1:2000) or ALP98 (1:1000) antibody followed by incubation with goat anti-mouse HRP-conjugated secondary antibodies diluted 1:2000 (The Jackson Laboratory) and the tetramethylbenzidine (TMB) substrate.

    Techniques: Recombinant, Incubation, Western Blot, Binding Assay, Enzyme-linked Immunosorbent Assay

    Integrin β5 protein expression is increased on TGFβ-induced Mfs. HCFs were treated with TGFβ1 or FGF-2 plus heparin for 72 hours and analyzed for β5 and β3 cell-surface expression. (A) Flow cytometry (B) Cell-surface biotinylation; lysates were immunoprecipitated (IP) with antibody against integrin β5, separated by SDS-PAGE and probed with streptavidin-HRP to detect biotinylated proteins. Bands were identified by molecular weight. GAPDH indicates equal protein input for IP. Quantification of the β5 band relative to GAPDH input control is shown. (C) Real-time PCR for β5, β3, and uPAR at Day 1 and Day 7 after TGFβ1 treatment. *p value

    Journal: PLoS ONE

    Article Title: Degradation of Internalized ?v?5 Integrin Is Controlled by uPAR Bound uPA: Effect on ?1 Integrin Activity and ?-SMA Stress Fiber Assembly

    doi: 10.1371/journal.pone.0033915

    Figure Lengend Snippet: Integrin β5 protein expression is increased on TGFβ-induced Mfs. HCFs were treated with TGFβ1 or FGF-2 plus heparin for 72 hours and analyzed for β5 and β3 cell-surface expression. (A) Flow cytometry (B) Cell-surface biotinylation; lysates were immunoprecipitated (IP) with antibody against integrin β5, separated by SDS-PAGE and probed with streptavidin-HRP to detect biotinylated proteins. Bands were identified by molecular weight. GAPDH indicates equal protein input for IP. Quantification of the β5 band relative to GAPDH input control is shown. (C) Real-time PCR for β5, β3, and uPAR at Day 1 and Day 7 after TGFβ1 treatment. *p value

    Article Snippet: HRP-conjugated anti-Streptavidin antibody and all HRP-conjugated secondary antibodies were from Jackson Laboratories.

    Techniques: Expressing, Flow Cytometry, Cytometry, Immunoprecipitation, SDS Page, Molecular Weight, Real-time Polymerase Chain Reaction

    β3 and TGFβRII expression is increased in PAI-1 KO cells. A , cell surface expression. WT and KO cells were biotinylated on the cell surface before lysis and immunoprecipitated for β3 or TGFβRII. Western blots were probed with streptavidin-HRP ( SA-HRP ) to detect biotinylated proteins. Bands were identified by molecular weight. B , total expression. WT and KO cell lysates were Western blotted for β3 or TGFβRII. Vimentin controls confirm equal loading (densitometry).

    Journal: The Journal of Biological Chemistry

    Article Title: Plasminogen Activator Inhibitor-1 Regulates Integrin ?v?3 Expression and Autocrine Transforming Growth Factor ? Signaling *

    doi: 10.1074/jbc.M109.018804

    Figure Lengend Snippet: β3 and TGFβRII expression is increased in PAI-1 KO cells. A , cell surface expression. WT and KO cells were biotinylated on the cell surface before lysis and immunoprecipitated for β3 or TGFβRII. Western blots were probed with streptavidin-HRP ( SA-HRP ) to detect biotinylated proteins. Bands were identified by molecular weight. B , total expression. WT and KO cell lysates were Western blotted for β3 or TGFβRII. Vimentin controls confirm equal loading (densitometry).

    Article Snippet: HRP-conjugated anti-Streptavidin antibodies and all HRP-conjugated secondary antibodies were from Jackson Laboratories (Bar Harbor, ME).

    Techniques: Expressing, Lysis, Immunoprecipitation, Western Blot, Molecular Weight