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Proteintech hrp conjugated secondary antibodies
Hrp Conjugated Secondary Antibodies, supplied by Proteintech, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hrp conjugated secondary antibodies/product/Proteintech
Average 99 stars, based on 16 article reviews
Price from $9.99 to $1999.99
hrp conjugated secondary antibodies - by Bioz Stars, 2020-07
99/100 stars

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Article Title: miR-150 is downregulated in osteosarcoma and suppresses cell proliferation, migration and invasion by targeting ROCK1
Article Snippet: .. Following incubation, specific secondary antibodies coupled to horseradish peroxidase (catalog., 15134-1-AP; dilution, 1:1,000; ProteinTech, Inc.) were used to detect the bands at room temperature. .. Evaluation of the expression of proteins was performed using ImageJ version 1.38 (National Institutes of Health, Bethesda, MD, USA).

other:

Article Title: Cantharidin suppresses cell growth and migration, and activates autophagy in human non-small cell lung cancer cells
Article Snippet: Primary antibodies against rabbit B cell lymphoma (Bcl)-2 (12789-1-AP), rabbit Bcl-2-associated X protein (Bax; 50599-2-Ig), mouse cyclin D1 (60186-1-Ig), rabbit Microtubule-associated protein 1A/1B-light chain 3 (LC3; 14600-1-AP), rabbit Beclin-1 (11306-1-AP), rabbit p62 (18420-1-AP), mouse GAPDH (60004-1-Ig), anti-rabbit (15134-1-AP) or anti-mouse (30000-0-AP) IgG-horseradish peroxidase-conjugated antibodies and the enhanced chemiluminescence (ECL) detection system were purchased from ProteinTech Group, Inc. (Wuhan, China).

Article Title: Increased expression of Myc-interacting zinc finger protein 1 in APP/PS1 mice
Article Snippet: Alexa Fluor 555-labeled donkey anti-mouse IgG (H+L) (1:200; A0460) and Alexa Fluor 488-labeled goat anti-rabbit IgG (H+L) (1:200; A0423) were from Beyotime Institute of Biotechnology (Haimen, China), and horseradish peroxidase (HRP)-conjugated anti-rabbit secondary antibody (151341AP; 1:5,000) and HRP-conjugated anti-mouse secondary antibody (HRP66008; 1:5,000) were from ProteinTech Group, Inc. (Chicago, IL, USA).

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  • 85
    Proteintech rabbit anti rat igg hrp conjugated secondary antibody
    Antibody titers of <t>IgG</t> induced by Cs Pmy measured by ELISA. Briefly, 1 µg/well recombinant pET-26b- Cs Pmy protein was coated on the plates and blocked with 5% skimmed milk. The plate was incubated with different dilutions of the immune sera (week 6) raised by pET-26b- Cs Pmy and pcDNA- Cs Pmy. Rat sera immunized with PBS and pcDNA were measured under the same conditions as negative controls. <t>HRP-conjugated</t> IgG (1∶20000 dilutions) was used as the secondary antibodies. The reactions were developed with substrate solution TMB, stopped by 2 M H 2 SO4 and measured at measured at 450 nm. (A) Antibody titers of IgG induced by pET-26b- Cs Pmy. (B) Antibody titers of IgG induced by pcDNA- Cs Pmy.
    Rabbit Anti Rat Igg Hrp Conjugated Secondary Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti rat igg hrp conjugated secondary antibody/product/Proteintech
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    rabbit anti rat igg hrp conjugated secondary antibody - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    91
    Proteintech horseradish peroxidase hrp conjugated secondary anti rabbit
    Antibody titers of <t>IgG</t> induced by Cs Pmy measured by ELISA. Briefly, 1 µg/well recombinant pET-26b- Cs Pmy protein was coated on the plates and blocked with 5% skimmed milk. The plate was incubated with different dilutions of the immune sera (week 6) raised by pET-26b- Cs Pmy and pcDNA- Cs Pmy. Rat sera immunized with PBS and pcDNA were measured under the same conditions as negative controls. <t>HRP-conjugated</t> IgG (1∶20000 dilutions) was used as the secondary antibodies. The reactions were developed with substrate solution TMB, stopped by 2 M H 2 SO4 and measured at measured at 450 nm. (A) Antibody titers of IgG induced by pET-26b- Cs Pmy. (B) Antibody titers of IgG induced by pcDNA- Cs Pmy.
    Horseradish Peroxidase Hrp Conjugated Secondary Anti Rabbit, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase hrp conjugated secondary anti rabbit/product/Proteintech
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase hrp conjugated secondary anti rabbit - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    93
    Proteintech hrp conjugated secondary antibodies
    Antibody titers of <t>IgG</t> induced by Cs Pmy measured by ELISA. Briefly, 1 µg/well recombinant pET-26b- Cs Pmy protein was coated on the plates and blocked with 5% skimmed milk. The plate was incubated with different dilutions of the immune sera (week 6) raised by pET-26b- Cs Pmy and pcDNA- Cs Pmy. Rat sera immunized with PBS and pcDNA were measured under the same conditions as negative controls. <t>HRP-conjugated</t> IgG (1∶20000 dilutions) was used as the secondary antibodies. The reactions were developed with substrate solution TMB, stopped by 2 M H 2 SO4 and measured at measured at 450 nm. (A) Antibody titers of IgG induced by pET-26b- Cs Pmy. (B) Antibody titers of IgG induced by pcDNA- Cs Pmy.
    Hrp Conjugated Secondary Antibodies, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated secondary antibodies/product/Proteintech
    Average 93 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated secondary antibodies - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

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    Antibody titers of IgG induced by Cs Pmy measured by ELISA. Briefly, 1 µg/well recombinant pET-26b- Cs Pmy protein was coated on the plates and blocked with 5% skimmed milk. The plate was incubated with different dilutions of the immune sera (week 6) raised by pET-26b- Cs Pmy and pcDNA- Cs Pmy. Rat sera immunized with PBS and pcDNA were measured under the same conditions as negative controls. HRP-conjugated IgG (1∶20000 dilutions) was used as the secondary antibodies. The reactions were developed with substrate solution TMB, stopped by 2 M H 2 SO4 and measured at measured at 450 nm. (A) Antibody titers of IgG induced by pET-26b- Cs Pmy. (B) Antibody titers of IgG induced by pcDNA- Cs Pmy.

    Journal: PLoS ONE

    Article Title: Identification and Characterization of Paramyosin from Cyst Wall of Metacercariae Implicated Protective Efficacy against Clonorchis sinensis Infection

    doi: 10.1371/journal.pone.0033703

    Figure Lengend Snippet: Antibody titers of IgG induced by Cs Pmy measured by ELISA. Briefly, 1 µg/well recombinant pET-26b- Cs Pmy protein was coated on the plates and blocked with 5% skimmed milk. The plate was incubated with different dilutions of the immune sera (week 6) raised by pET-26b- Cs Pmy and pcDNA- Cs Pmy. Rat sera immunized with PBS and pcDNA were measured under the same conditions as negative controls. HRP-conjugated IgG (1∶20000 dilutions) was used as the secondary antibodies. The reactions were developed with substrate solution TMB, stopped by 2 M H 2 SO4 and measured at measured at 450 nm. (A) Antibody titers of IgG induced by pET-26b- Cs Pmy. (B) Antibody titers of IgG induced by pcDNA- Cs Pmy.

    Article Snippet: Subsequently, the membrane was followed by incubation with rabbit anti-rat IgG HRP-conjugated secondary antibody (1∶2000 dilutions in 1% BSA-PBS, Proteintech Group, Chicago, USA) at room temperature for 1 h. The protein bands were visualized by enhanced chemiluminescence (ECL) method.

    Techniques: Enzyme-linked Immunosorbent Assay, Recombinant, Positron Emission Tomography, Incubation

    Identification of Cs Pmy by SDS-PAGE and Western blot analysis. Protein molecular weight marker (M), purified pET-26b- Cs Pmy protein (lane 1), TWE of adult worm (lane 2), TWE of metacercaria (lane 3), TWE of cercaria (lane 4), TWE of egg (lane 5), cyst wall proteins of metacercaria (lane 6), and soluble tegumental components of adult worm (lane 7). (A) 8% SDS-PAGE. (B) Western blot analysis. Corresponding proteins were subjected to 8% SDS-PAGE and immobilized onto the membrane, then the membrane was incubated with anti-pET-26b- Cs Pmy rat serum (1∶2000 dilutions) at room temperature for 2 h. Subsequently, the membrane was followed by incubation with rabbit anti-rat IgG HRP-conjugated secondary antibody (1∶2000 dilutions) at room temperature for 1 h. 2 µg of purified pET-26b- Cs Pmy protein and 10 µg of TWE were loaded per lane. SDS-PAGE was visualized by Coomassie Blues staining and the protein bands that might be native paramyosin in different life stages were indicated with arrows. Western blot was visualized by ECL method, the detected protein bands were around 100 kDa.

    Journal: PLoS ONE

    Article Title: Identification and Characterization of Paramyosin from Cyst Wall of Metacercariae Implicated Protective Efficacy against Clonorchis sinensis Infection

    doi: 10.1371/journal.pone.0033703

    Figure Lengend Snippet: Identification of Cs Pmy by SDS-PAGE and Western blot analysis. Protein molecular weight marker (M), purified pET-26b- Cs Pmy protein (lane 1), TWE of adult worm (lane 2), TWE of metacercaria (lane 3), TWE of cercaria (lane 4), TWE of egg (lane 5), cyst wall proteins of metacercaria (lane 6), and soluble tegumental components of adult worm (lane 7). (A) 8% SDS-PAGE. (B) Western blot analysis. Corresponding proteins were subjected to 8% SDS-PAGE and immobilized onto the membrane, then the membrane was incubated with anti-pET-26b- Cs Pmy rat serum (1∶2000 dilutions) at room temperature for 2 h. Subsequently, the membrane was followed by incubation with rabbit anti-rat IgG HRP-conjugated secondary antibody (1∶2000 dilutions) at room temperature for 1 h. 2 µg of purified pET-26b- Cs Pmy protein and 10 µg of TWE were loaded per lane. SDS-PAGE was visualized by Coomassie Blues staining and the protein bands that might be native paramyosin in different life stages were indicated with arrows. Western blot was visualized by ECL method, the detected protein bands were around 100 kDa.

    Article Snippet: Subsequently, the membrane was followed by incubation with rabbit anti-rat IgG HRP-conjugated secondary antibody (1∶2000 dilutions in 1% BSA-PBS, Proteintech Group, Chicago, USA) at room temperature for 1 h. The protein bands were visualized by enhanced chemiluminescence (ECL) method.

    Techniques: SDS Page, Western Blot, Molecular Weight, Marker, Purification, Positron Emission Tomography, Incubation, Staining