Structured Review

Beyotime hrp conjugated secondary antibodies
Hrp Conjugated Secondary Antibodies, supplied by Beyotime, used in various techniques. Bioz Stars score: 94/100, based on 130 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hrp conjugated secondary antibodies/product/Beyotime
Average 94 stars, based on 130 article reviews
Price from $9.99 to $1999.99
hrp conjugated secondary antibodies - by Bioz Stars, 2020-07
94/100 stars

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Incubation:

Article Title: Dysregulated erythropoietin-producing hepatocellular receptor A2 (EphA2) is involved in tubal pregnancy via regulating cell adhesion of the Fallopian tube epithelial cells
Article Snippet: .. After washing, the membranes were then incubated for 1 h with the appropriate HRP-conjugated secondary antibodies, goat anti-rabbit IgG (1:800; A0208; Beyotime Biotechnology Co., Ltd., Shanghai, China) or rabbit anti-mouse IgG (1:1000; D031402; Dako Cytomatin, Glostrup, Denmark). .. Protein bands were identified using ECL (Pierce, Rockford, USA).

Article Title: Knockdown of versican V1 induces a severe inflammatory response in LPS-induced acute lung injury via the TLR2-NF-κB signaling pathway in C57BL/6J mice
Article Snippet: .. The membranes were incubated with HRP-conjugated secondary antibodies (1:1,000; Beyotime Institute of Biotechnology). .. The immunoreactive signals were visualized using an enhanced chemiluminescence detection system (Beyotime Institute of Biotechnology).

Article Title: Pretreatment with AM1241 Enhances the Analgesic Effect of Intrathecally Administrated Mesenchymal Stem Cells
Article Snippet: .. This was followed by incubation with HRP-conjugated secondary antibodies: goat anti-mouse or goat anti-rabbit IgG (1 : 5000, Beyotime, China) for 1 h at room temperature. ..

Article Title: Sirt7 promotes gastric cancer growth and inhibits apoptosis by epigenetically inhibiting miR-34a
Article Snippet: .. The membranes were probed with primary antibody for Sirt7 (Santa Cruz), Caspase-3 (Santa Cruz), PARP (Abcam), Bax (Abcam), Bim (Abcam), Bcl-2(Santa Cruz), Mcl-1(Abcam), H3(Santa Cruz), H3K18ac (Santa Cruz) or β-actin (Abcam) at 4°C overnight, and then the membranes were washed and incubated with HRP-conjugated secondary antibody (in 5% fat-free milk) for 2 hours and finally visualized using Chemiluminescent ECL reagent (Beyotime). .. Apoptosis assay Apoptosis analysis was conducted with an Annexin V-FITC Apoptosis Detection Kit (Becton Dickinson) according to the manufacturer's protocol.

Article Title: Over-expression of ARHGAP18 suppressed cell proliferation, migration, invasion, and tumor growth in gastric cancer by restraining over-activation of MAPK signaling pathways
Article Snippet: .. After washing with TBST three times, 5 min each time, the membranes were incubated with HRP-conjugated secondary antibody (1:2,000; Beyotime) in TBST at room temperature for 1 h. Equal sample loading was confirmed using GAPDH. .. Protein expression was detected using enhanced chemiluminescence (Thermo Fisher Scientific).

Article Title: TRIM6 promotes colorectal cancer cells proliferation and response to thiostrepton by TIS21/FoxM1
Article Snippet: .. After incubation with HRP-conjugated secondary antibodies (Beyotime Biotech.), the signal was detected using an ECL kit (Pierce, Rockford, IL, USA). .. Construction of plasmids TRIM6, TRIM6 with substitute mutation of C15A and FoxM1 cDNA was cloned into the pcDNA3.1-myc vector (Life Technology) to express Myc-tagged TRIM6, TRIM6 E3 catalytic mutant (C15A) and FoxM1, respectively.

Imaging:

Article Title: Repeated positive acceleration exposure exacerbates endothelial dysfunction in high-fat-diet-induced hyperlipidemic rats
Article Snippet: .. The membranes were then washed in PBS 3 times and incubated with HRP conjugated secondary antibodies (1 : 10000, Beyotime) at room temperature for 1 h and scanned using the AlphaImager HP fluorescent and visible light gel imaging system (ProteinSimple, San Jose, CA, USA). .. The grey value was analyzed using IPP6 software.

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  • 92
    Beyotime hrp conjugated goat anti rabbit
    Specificities of anti-GnRHR1 and anti-GnRHR2 antisera against recombinant proteins as determined by Western blot analysis. The recombinant proteins (50 ng) or COS-7 cell extracts (100 μg) were separated on 12% SDS-PAGE gels, processed routinely, and immnoreacted with the rabbit anti-GnRHR1 antiserum [1:2,000 dilution; (A) ], the mouse anti-GnRHR2 antiserum [1:1,000 dilution; (B) ], the anti-GnRHR1 antiserum pre-adsorbed with 10 ug/mL of recombinant GnRHR1 expressed in transfected COS-7 cells (C) , or the anti-GnRHR2 antiserum pre-adsorbed with 10 ug/mL of recombinant GnRHR2 expressed in transfected COS-7 cells (D) . The secondary antibody was 1:1,000 diluted horseradish peroxidase <t>(HRP)-conjugated</t> goat anti-rabbit or anti-mouse <t>IgG</t> (H+L) (Beyotime, Shanghai, China), and the blots were visualized using BeyoECL Plus kit (Beyotime). GnRHR1-antigen, the recombinant GnRHR1 polypeptide used to immunize rabbit; GnRHR1-TRX, the region corresponding to GnRHR1 antigen expressed as thioredoxin (TRX) fusion protein; GnRHR2-TRX, GnRHR2 polypeptide region encompassing the synthetic antigen peptide expressed as TRX fusion protein. GnRHR1 and GnRHR2, the recombinant full-length ricefield eel GnRHR1 and GnRHR2 proteins expressed in transiently transfected COS-7 cells. pcDNA, extracts of COS-7 cells transfected with the empty vector pcDNA3.0. COS-7: extracts of COS-7 cells.
    Hrp Conjugated Goat Anti Rabbit, supplied by Beyotime, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated goat anti rabbit/product/Beyotime
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated goat anti rabbit - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    91
    Beyotime hrp conjugated goat anti rabbit igg secondary antibody
    Specificities of anti-GnRHR1 and anti-GnRHR2 antisera against recombinant proteins as determined by Western blot analysis. The recombinant proteins (50 ng) or COS-7 cell extracts (100 μg) were separated on 12% SDS-PAGE gels, processed routinely, and immnoreacted with the rabbit anti-GnRHR1 antiserum [1:2,000 dilution; (A) ], the mouse anti-GnRHR2 antiserum [1:1,000 dilution; (B) ], the anti-GnRHR1 antiserum pre-adsorbed with 10 ug/mL of recombinant GnRHR1 expressed in transfected COS-7 cells (C) , or the anti-GnRHR2 antiserum pre-adsorbed with 10 ug/mL of recombinant GnRHR2 expressed in transfected COS-7 cells (D) . The secondary antibody was 1:1,000 diluted horseradish peroxidase <t>(HRP)-conjugated</t> goat anti-rabbit or anti-mouse <t>IgG</t> (H+L) (Beyotime, Shanghai, China), and the blots were visualized using BeyoECL Plus kit (Beyotime). GnRHR1-antigen, the recombinant GnRHR1 polypeptide used to immunize rabbit; GnRHR1-TRX, the region corresponding to GnRHR1 antigen expressed as thioredoxin (TRX) fusion protein; GnRHR2-TRX, GnRHR2 polypeptide region encompassing the synthetic antigen peptide expressed as TRX fusion protein. GnRHR1 and GnRHR2, the recombinant full-length ricefield eel GnRHR1 and GnRHR2 proteins expressed in transiently transfected COS-7 cells. pcDNA, extracts of COS-7 cells transfected with the empty vector pcDNA3.0. COS-7: extracts of COS-7 cells.
    Hrp Conjugated Goat Anti Rabbit Igg Secondary Antibody, supplied by Beyotime, used in various techniques. Bioz Stars score: 91/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated goat anti rabbit igg secondary antibody/product/Beyotime
    Average 91 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated goat anti rabbit igg secondary antibody - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    92
    Beyotime horseradish peroxide conjugated goat anti rabbit immunoglobulin g h l secondary antibody
    Specificities of anti-GnRHR1 and anti-GnRHR2 antisera against recombinant proteins as determined by Western blot analysis. The recombinant proteins (50 ng) or COS-7 cell extracts (100 μg) were separated on 12% SDS-PAGE gels, processed routinely, and immnoreacted with the rabbit anti-GnRHR1 antiserum [1:2,000 dilution; (A) ], the mouse anti-GnRHR2 antiserum [1:1,000 dilution; (B) ], the anti-GnRHR1 antiserum pre-adsorbed with 10 ug/mL of recombinant GnRHR1 expressed in transfected COS-7 cells (C) , or the anti-GnRHR2 antiserum pre-adsorbed with 10 ug/mL of recombinant GnRHR2 expressed in transfected COS-7 cells (D) . The secondary antibody was 1:1,000 diluted horseradish peroxidase <t>(HRP)-conjugated</t> goat anti-rabbit or anti-mouse <t>IgG</t> (H+L) (Beyotime, Shanghai, China), and the blots were visualized using BeyoECL Plus kit (Beyotime). GnRHR1-antigen, the recombinant GnRHR1 polypeptide used to immunize rabbit; GnRHR1-TRX, the region corresponding to GnRHR1 antigen expressed as thioredoxin (TRX) fusion protein; GnRHR2-TRX, GnRHR2 polypeptide region encompassing the synthetic antigen peptide expressed as TRX fusion protein. GnRHR1 and GnRHR2, the recombinant full-length ricefield eel GnRHR1 and GnRHR2 proteins expressed in transiently transfected COS-7 cells. pcDNA, extracts of COS-7 cells transfected with the empty vector pcDNA3.0. COS-7: extracts of COS-7 cells.
    Horseradish Peroxide Conjugated Goat Anti Rabbit Immunoglobulin G H L Secondary Antibody, supplied by Beyotime, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxide conjugated goat anti rabbit immunoglobulin g h l secondary antibody/product/Beyotime
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxide conjugated goat anti rabbit immunoglobulin g h l secondary antibody - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    93
    Beyotime horseradish peroxide conjugated antibody
    Specificities of anti-GnRHR1 and anti-GnRHR2 antisera against recombinant proteins as determined by Western blot analysis. The recombinant proteins (50 ng) or COS-7 cell extracts (100 μg) were separated on 12% SDS-PAGE gels, processed routinely, and immnoreacted with the rabbit anti-GnRHR1 antiserum [1:2,000 dilution; (A) ], the mouse anti-GnRHR2 antiserum [1:1,000 dilution; (B) ], the anti-GnRHR1 antiserum pre-adsorbed with 10 ug/mL of recombinant GnRHR1 expressed in transfected COS-7 cells (C) , or the anti-GnRHR2 antiserum pre-adsorbed with 10 ug/mL of recombinant GnRHR2 expressed in transfected COS-7 cells (D) . The secondary antibody was 1:1,000 diluted horseradish peroxidase <t>(HRP)-conjugated</t> goat anti-rabbit or anti-mouse <t>IgG</t> (H+L) (Beyotime, Shanghai, China), and the blots were visualized using BeyoECL Plus kit (Beyotime). GnRHR1-antigen, the recombinant GnRHR1 polypeptide used to immunize rabbit; GnRHR1-TRX, the region corresponding to GnRHR1 antigen expressed as thioredoxin (TRX) fusion protein; GnRHR2-TRX, GnRHR2 polypeptide region encompassing the synthetic antigen peptide expressed as TRX fusion protein. GnRHR1 and GnRHR2, the recombinant full-length ricefield eel GnRHR1 and GnRHR2 proteins expressed in transiently transfected COS-7 cells. pcDNA, extracts of COS-7 cells transfected with the empty vector pcDNA3.0. COS-7: extracts of COS-7 cells.
    Horseradish Peroxide Conjugated Antibody, supplied by Beyotime, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxide conjugated antibody/product/Beyotime
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxide conjugated antibody - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    Image Search Results


    Specificities of anti-GnRHR1 and anti-GnRHR2 antisera against recombinant proteins as determined by Western blot analysis. The recombinant proteins (50 ng) or COS-7 cell extracts (100 μg) were separated on 12% SDS-PAGE gels, processed routinely, and immnoreacted with the rabbit anti-GnRHR1 antiserum [1:2,000 dilution; (A) ], the mouse anti-GnRHR2 antiserum [1:1,000 dilution; (B) ], the anti-GnRHR1 antiserum pre-adsorbed with 10 ug/mL of recombinant GnRHR1 expressed in transfected COS-7 cells (C) , or the anti-GnRHR2 antiserum pre-adsorbed with 10 ug/mL of recombinant GnRHR2 expressed in transfected COS-7 cells (D) . The secondary antibody was 1:1,000 diluted horseradish peroxidase (HRP)-conjugated goat anti-rabbit or anti-mouse IgG (H+L) (Beyotime, Shanghai, China), and the blots were visualized using BeyoECL Plus kit (Beyotime). GnRHR1-antigen, the recombinant GnRHR1 polypeptide used to immunize rabbit; GnRHR1-TRX, the region corresponding to GnRHR1 antigen expressed as thioredoxin (TRX) fusion protein; GnRHR2-TRX, GnRHR2 polypeptide region encompassing the synthetic antigen peptide expressed as TRX fusion protein. GnRHR1 and GnRHR2, the recombinant full-length ricefield eel GnRHR1 and GnRHR2 proteins expressed in transiently transfected COS-7 cells. pcDNA, extracts of COS-7 cells transfected with the empty vector pcDNA3.0. COS-7: extracts of COS-7 cells.

    Journal: Frontiers in Endocrinology

    Article Title: A Type IIb, but Not Type IIa, GnRH Receptor Mediates GnRH-Induced Release of Growth Hormone in the Ricefield Eel

    doi: 10.3389/fendo.2018.00721

    Figure Lengend Snippet: Specificities of anti-GnRHR1 and anti-GnRHR2 antisera against recombinant proteins as determined by Western blot analysis. The recombinant proteins (50 ng) or COS-7 cell extracts (100 μg) were separated on 12% SDS-PAGE gels, processed routinely, and immnoreacted with the rabbit anti-GnRHR1 antiserum [1:2,000 dilution; (A) ], the mouse anti-GnRHR2 antiserum [1:1,000 dilution; (B) ], the anti-GnRHR1 antiserum pre-adsorbed with 10 ug/mL of recombinant GnRHR1 expressed in transfected COS-7 cells (C) , or the anti-GnRHR2 antiserum pre-adsorbed with 10 ug/mL of recombinant GnRHR2 expressed in transfected COS-7 cells (D) . The secondary antibody was 1:1,000 diluted horseradish peroxidase (HRP)-conjugated goat anti-rabbit or anti-mouse IgG (H+L) (Beyotime, Shanghai, China), and the blots were visualized using BeyoECL Plus kit (Beyotime). GnRHR1-antigen, the recombinant GnRHR1 polypeptide used to immunize rabbit; GnRHR1-TRX, the region corresponding to GnRHR1 antigen expressed as thioredoxin (TRX) fusion protein; GnRHR2-TRX, GnRHR2 polypeptide region encompassing the synthetic antigen peptide expressed as TRX fusion protein. GnRHR1 and GnRHR2, the recombinant full-length ricefield eel GnRHR1 and GnRHR2 proteins expressed in transiently transfected COS-7 cells. pcDNA, extracts of COS-7 cells transfected with the empty vector pcDNA3.0. COS-7: extracts of COS-7 cells.

    Article Snippet: Then slides were applied to the secondary antibody, HRP-conjugated goat anti-rabbit or anti-mouse IgG (H+L) (1:1,000 dilution; Beyotime), and finally visualized with 3,3'-diaminobenzidine (DAB) solution, mounted, and digitally photographed with a microscope (Eclipse Ni-U, Nikon, Japan).

    Techniques: Recombinant, Western Blot, SDS Page, Transfection, Plasmid Preparation

    Specificities of GnRHR1 and GnRHR2 immunoreactivities in the pituitary of male ricefield eels as determined by immunohistochemical analysis. Sagittal sections of the pituitary gland were immunoreacted with the rabbit anti-GnRHR1 antiserum [1:500 dilution; (A) ], the pre-adsorbed anti-GnRHR1 antiserum by 10 ug/mL of recombinant GnRHR1 expressed in transfected COS-7 cells (B) , the mouse anti-GnRHR2 antiserum [1:500 dilution; (C) ], the pre-adsorbed anti-GnRHR2 antiserum by 10 ug/mL of recombinant GnRHR2 expressed in transfected COS-7 cells (D) , or the mixture of the rabbit anti-GnRHR1 (1:500 dilution) with the mouse anti-GnRHR2 (1:500 dilution) antisera (E,F) . The secondary antibody was 1:1,000 diluted horseradish peroxidase (HRP)-conjugated goat anti-rabbit or anti-mouse IgG (H+L) (Beyotime, Shanghai, China) for (A–D) , and the mixture of Alexa Flour 488-labeled goat anti-rabbit IgG (H+L) (1:500 dilution) and Cy3-labeled goat anti-mouse IgG (H+L) (1:500 dilution) for (E) . DAPI was used to stain the nuclei blue. Immunostaining (brown) in (A–D) was visualized by the DAB chromogen and counterstained with hematoxylin. The black triangles and stars indicate the adenohypophysis and neurohypophysis tissues, respectively. The image of (E) was captured and analyzed with a Nikon i-E confocal microscope equipped with a CSU-W1 spinning-disk head (Yokogawa, Tokyo, Japan) for immunofluorescent staining of GnRHR1 (green) and GnRHR2 (red). (F) is the higher magnification of the boxed area in (E) . Sagittal sections of ricefield eel pituitary glands were shown here with the rostral (anterior) to the left. Scale bar = 50 μm except the image of (F) was 10 μm.

    Journal: Frontiers in Endocrinology

    Article Title: A Type IIb, but Not Type IIa, GnRH Receptor Mediates GnRH-Induced Release of Growth Hormone in the Ricefield Eel

    doi: 10.3389/fendo.2018.00721

    Figure Lengend Snippet: Specificities of GnRHR1 and GnRHR2 immunoreactivities in the pituitary of male ricefield eels as determined by immunohistochemical analysis. Sagittal sections of the pituitary gland were immunoreacted with the rabbit anti-GnRHR1 antiserum [1:500 dilution; (A) ], the pre-adsorbed anti-GnRHR1 antiserum by 10 ug/mL of recombinant GnRHR1 expressed in transfected COS-7 cells (B) , the mouse anti-GnRHR2 antiserum [1:500 dilution; (C) ], the pre-adsorbed anti-GnRHR2 antiserum by 10 ug/mL of recombinant GnRHR2 expressed in transfected COS-7 cells (D) , or the mixture of the rabbit anti-GnRHR1 (1:500 dilution) with the mouse anti-GnRHR2 (1:500 dilution) antisera (E,F) . The secondary antibody was 1:1,000 diluted horseradish peroxidase (HRP)-conjugated goat anti-rabbit or anti-mouse IgG (H+L) (Beyotime, Shanghai, China) for (A–D) , and the mixture of Alexa Flour 488-labeled goat anti-rabbit IgG (H+L) (1:500 dilution) and Cy3-labeled goat anti-mouse IgG (H+L) (1:500 dilution) for (E) . DAPI was used to stain the nuclei blue. Immunostaining (brown) in (A–D) was visualized by the DAB chromogen and counterstained with hematoxylin. The black triangles and stars indicate the adenohypophysis and neurohypophysis tissues, respectively. The image of (E) was captured and analyzed with a Nikon i-E confocal microscope equipped with a CSU-W1 spinning-disk head (Yokogawa, Tokyo, Japan) for immunofluorescent staining of GnRHR1 (green) and GnRHR2 (red). (F) is the higher magnification of the boxed area in (E) . Sagittal sections of ricefield eel pituitary glands were shown here with the rostral (anterior) to the left. Scale bar = 50 μm except the image of (F) was 10 μm.

    Article Snippet: Then slides were applied to the secondary antibody, HRP-conjugated goat anti-rabbit or anti-mouse IgG (H+L) (1:1,000 dilution; Beyotime), and finally visualized with 3,3'-diaminobenzidine (DAB) solution, mounted, and digitally photographed with a microscope (Eclipse Ni-U, Nikon, Japan).

    Techniques: Immunohistochemistry, Recombinant, Transfection, Labeling, Staining, Immunostaining, Microscopy

    GnRH1, GnRH2, and GnRH3 immunostaining in the brain and pituitary of ricefield eels. Sagittal sections of the brains together with the pituitary gland of ricefield eels were immunoreacted with primary antibodies, the rabbit polyclonal antibody AS-691 for GnRH1 [1:7,000 dilution; (B,C,H) ], the rabbit polyclonal antibody 675 for GnRH2 [1:2,000 dilution; (D,I) ], or the mouse monoclonal antibody LRH13 for GnRH3 [1:2,000 dilution; (E–G,J) ]. After incubation with primary antibodies for 40 h at 4°C, sections were then exposed to the secondary antibody, HRP-conjugated goat anti-rabbit or anti-mouse IgG (H+L) (1:1,000 dilution; Beyotime, Shanghai, China), and finally visualized with 3,3'-diaminobenzidine (DAB) solution, mounted, and digitally photographed with a Nikon Eclipse Ni-U microscope (Japan). (A) , the schematic diagram of the ricefield eel brain and pituitary. Red, green, and blue dots in (A) represent GnRH1, GnRH2, and GnRH3 immunoreactive neurons, respectively. OB, olfactory bulbs; TEL, telencephalon; VT, ventral telencephalon; POA, preoptic area; OT, optic tectum-thalamus; MT, midbrain tegmentum; Hyp, hypothalamus; Pit, pituitary; Ce, cerebellum; MO, medulla oblongata. Scale bar = 50 μm except where it is specifically designated otherwise.

    Journal: Frontiers in Endocrinology

    Article Title: A Type IIb, but Not Type IIa, GnRH Receptor Mediates GnRH-Induced Release of Growth Hormone in the Ricefield Eel

    doi: 10.3389/fendo.2018.00721

    Figure Lengend Snippet: GnRH1, GnRH2, and GnRH3 immunostaining in the brain and pituitary of ricefield eels. Sagittal sections of the brains together with the pituitary gland of ricefield eels were immunoreacted with primary antibodies, the rabbit polyclonal antibody AS-691 for GnRH1 [1:7,000 dilution; (B,C,H) ], the rabbit polyclonal antibody 675 for GnRH2 [1:2,000 dilution; (D,I) ], or the mouse monoclonal antibody LRH13 for GnRH3 [1:2,000 dilution; (E–G,J) ]. After incubation with primary antibodies for 40 h at 4°C, sections were then exposed to the secondary antibody, HRP-conjugated goat anti-rabbit or anti-mouse IgG (H+L) (1:1,000 dilution; Beyotime, Shanghai, China), and finally visualized with 3,3'-diaminobenzidine (DAB) solution, mounted, and digitally photographed with a Nikon Eclipse Ni-U microscope (Japan). (A) , the schematic diagram of the ricefield eel brain and pituitary. Red, green, and blue dots in (A) represent GnRH1, GnRH2, and GnRH3 immunoreactive neurons, respectively. OB, olfactory bulbs; TEL, telencephalon; VT, ventral telencephalon; POA, preoptic area; OT, optic tectum-thalamus; MT, midbrain tegmentum; Hyp, hypothalamus; Pit, pituitary; Ce, cerebellum; MO, medulla oblongata. Scale bar = 50 μm except where it is specifically designated otherwise.

    Article Snippet: Then slides were applied to the secondary antibody, HRP-conjugated goat anti-rabbit or anti-mouse IgG (H+L) (1:1,000 dilution; Beyotime), and finally visualized with 3,3'-diaminobenzidine (DAB) solution, mounted, and digitally photographed with a microscope (Eclipse Ni-U, Nikon, Japan).

    Techniques: Immunostaining, Incubation, Microscopy