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Bethyl hrp conjugated secondary antibodies
Hrp Conjugated Secondary Antibodies, supplied by Bethyl, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hrp conjugated secondary antibodies/product/Bethyl
Average 94 stars, based on 12 article reviews
Price from $9.99 to $1999.99
hrp conjugated secondary antibodies - by Bioz Stars, 2020-07
94/100 stars

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Article Title: Cloning, expression, purification and crystallization of Schizosaccharomyces pombe Set7, a putative histone methyltransferase
Article Snippet: .. The protein was concentrated to 6 mg ml−1 and confirmed by 15% SDS–PAGE stained with Coomassie Blue G-250 and Western blotting using HRP-conjugated α-HA antibody (A190-108P, Bethyl Laboratories, Montgomery, Texas, USA). .. SDS–PAGE and native PAGE tend to indicate homogenous S. pombe Set7 during the crystallization phases (Fig. 2 b ).

SDS Page:

Article Title: Cloning, expression, purification and crystallization of Schizosaccharomyces pombe Set7, a putative histone methyltransferase
Article Snippet: .. The protein was concentrated to 6 mg ml−1 and confirmed by 15% SDS–PAGE stained with Coomassie Blue G-250 and Western blotting using HRP-conjugated α-HA antibody (A190-108P, Bethyl Laboratories, Montgomery, Texas, USA). .. SDS–PAGE and native PAGE tend to indicate homogenous S. pombe Set7 during the crystallization phases (Fig. 2 b ).

Western Blot:

Article Title: Cloning, expression, purification and crystallization of Schizosaccharomyces pombe Set7, a putative histone methyltransferase
Article Snippet: .. The protein was concentrated to 6 mg ml−1 and confirmed by 15% SDS–PAGE stained with Coomassie Blue G-250 and Western blotting using HRP-conjugated α-HA antibody (A190-108P, Bethyl Laboratories, Montgomery, Texas, USA). .. SDS–PAGE and native PAGE tend to indicate homogenous S. pombe Set7 during the crystallization phases (Fig. 2 b ).

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  • 99
    Bethyl hrp conjugated goat anti porcine igg
    Mouse serum antiadhesin and antitoxin <t>IgG</t> antibody titers (log 10 scale). Anti-STa, -LT, -CFA/I, -CS1, -CS2, -CS3, -CS4/CS6, and -CS5/CS6 IgG antibodies in the serum samples of each mouse immunized with tagless CFA/I/II/IV-3xSTa N12S -mnLT R192G/L211A MEFA with Freund's incomplete adjuvant (•) or dmLT adjuvant (▽), or serum of each control mouse (○), were titrated in ELISAs. Heat-extracted fimbrial adhesin CFA/I, CS1, CS2, CS3, CS4/CS6, or CS5/CS6 (200 ng per well of a 2HB plate), STa-ovalbumin (10 ng per well of a Costar plate), or LT (100 ng per well of a 2HB plate; List Biological Laboratories, Inc.) and <t>HRP-conjugated</t> goat-anti-mouse IgG (1:3,300; the secondary antibodies) were used to titrate IgG antibodies specific to CFA/I, CS1, CS2, CS3, CS4/6, and CS5/6 and to STa and LT toxins. Each dot represents an IgG titer of a mouse, and bars indicated the mean titer of the group. The P values from Student t test indicate the significance of differences of antibody titers (log 10 scale) between two immunization groups.
    Hrp Conjugated Goat Anti Porcine Igg, supplied by Bethyl, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated goat anti porcine igg/product/Bethyl
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated goat anti porcine igg - by Bioz Stars, 2020-07
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    90
    Bethyl mouse igg3
    Human splenic ILCs activate MZ B cell-helper neutrophils via GM-CSF ( a ) IHC of spleen stained for CD117 (red) and CD66 (brown). Original magnification, ×20 or ×40 (inset). ( b ) Left: qRT-PCR of CSF2 (GM-CSF) and IL8 (IL-8) mRNAs from splenic ILCs, NK cells, macrophages (Mϕ), B cells and T cells. CSF2 and IL8 data are normalized to ACTB (β-actin) mRNA and presented as relative expression (RE) compared with that of fresh splenic NK cells. Right: ELISA of GM-CSF and IL-8 from splenic ILCs and NK cells cultured for 48 h with IL-1β and IL-7. ( c ) Flow cytometry of intracellular GMCSF and IL-8 in splenic CD127 + (Lin – CD117 + ) ILCs activated with or without PMA (P) plus ionomycin (I) for 4 h. ( d ) Flow cytometry of CD69, CD11b, CD24 and CD62L on Nc cells cultured for 24 h with or without splenic ILCs in the presence or absence of control <t>IgG1</t> or blocking anti-GM-CSF antibodies. Gray shading: isotype control. ( e ) Flow cytometry of viable DAPI – N c cells cultured with medium alone (Ctrl), GM-CSF, ILCs or ILC-derived conditioned medium (ILC-CM) in the presence or absence of IgG1 or anti-GM-CSF antibodies. ( f ) qRT-PCR of TNFSF13 (APRIL) mRNA from N c cells cultured for 24 h with medium alone (Ctrl), GM-CSF or ILC-CM. Results are normalized to ACTB mRNA and presented as RE compared with that of N c cells cultured with medium alone. ( g ) ELISA of IgA from splenic MZ B cells cultured for 5 d with or without N c cells, ILCs and/or ILC-CM. ( h ) ELISA of IgM, IgA and <t>IgG</t> from splenic MZ B cells cultured for 5 d with or without N BH cells, ILC-CM and/or N BH cells preconditioned with ILC-CM. ( i ) IFA of elastase (green) and DAPI-stained DNA (blue) in N c cells cultured for 3 h either with medium alone (Ctrl), GM-CSF, ILC-CM or LPS (original magnification, ×63) and quantification of NET-forming N c cells from nine ×10 fields from two independent experiments. Error bars, s.e.m.; * P
    Mouse Igg3, supplied by Bethyl, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse igg3 - by Bioz Stars, 2020-07
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    93
    Bethyl horseradish peroxidase conjugated goat human iga antibody
    Transitions of the fluorescence intensity in the well with the film-stack reaction fields with the micro-pillars array having different dimensions in relation in relation to <t>HRP-IgA</t> incubation time. All fluorescence intensities are the same with Figure 7 . Error bars are the maximum and minimum intensities.
    Horseradish Peroxidase Conjugated Goat Human Iga Antibody, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase conjugated goat human iga antibody/product/Bethyl
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    Mouse serum antiadhesin and antitoxin IgG antibody titers (log 10 scale). Anti-STa, -LT, -CFA/I, -CS1, -CS2, -CS3, -CS4/CS6, and -CS5/CS6 IgG antibodies in the serum samples of each mouse immunized with tagless CFA/I/II/IV-3xSTa N12S -mnLT R192G/L211A MEFA with Freund's incomplete adjuvant (•) or dmLT adjuvant (▽), or serum of each control mouse (○), were titrated in ELISAs. Heat-extracted fimbrial adhesin CFA/I, CS1, CS2, CS3, CS4/CS6, or CS5/CS6 (200 ng per well of a 2HB plate), STa-ovalbumin (10 ng per well of a Costar plate), or LT (100 ng per well of a 2HB plate; List Biological Laboratories, Inc.) and HRP-conjugated goat-anti-mouse IgG (1:3,300; the secondary antibodies) were used to titrate IgG antibodies specific to CFA/I, CS1, CS2, CS3, CS4/6, and CS5/6 and to STa and LT toxins. Each dot represents an IgG titer of a mouse, and bars indicated the mean titer of the group. The P values from Student t test indicate the significance of differences of antibody titers (log 10 scale) between two immunization groups.

    Journal: Infection and Immunity

    Article Title: Enterotoxigenic Escherichia coli Adhesin-Toxoid Multiepitope Fusion Antigen CFA/I/II/IV-3xSTaN12S-mnLTG192G/L211A-Derived Antibodies Inhibit Adherence of Seven Adhesins, Neutralize Enterotoxicity of LT and STa Toxins, and Protect Piglets against Diarrhea

    doi: 10.1128/IAI.00550-17

    Figure Lengend Snippet: Mouse serum antiadhesin and antitoxin IgG antibody titers (log 10 scale). Anti-STa, -LT, -CFA/I, -CS1, -CS2, -CS3, -CS4/CS6, and -CS5/CS6 IgG antibodies in the serum samples of each mouse immunized with tagless CFA/I/II/IV-3xSTa N12S -mnLT R192G/L211A MEFA with Freund's incomplete adjuvant (•) or dmLT adjuvant (▽), or serum of each control mouse (○), were titrated in ELISAs. Heat-extracted fimbrial adhesin CFA/I, CS1, CS2, CS3, CS4/CS6, or CS5/CS6 (200 ng per well of a 2HB plate), STa-ovalbumin (10 ng per well of a Costar plate), or LT (100 ng per well of a 2HB plate; List Biological Laboratories, Inc.) and HRP-conjugated goat-anti-mouse IgG (1:3,300; the secondary antibodies) were used to titrate IgG antibodies specific to CFA/I, CS1, CS2, CS3, CS4/6, and CS5/6 and to STa and LT toxins. Each dot represents an IgG titer of a mouse, and bars indicated the mean titer of the group. The P values from Student t test indicate the significance of differences of antibody titers (log 10 scale) between two immunization groups.

    Article Snippet: HRP-conjugated goat-anti-porcine IgG and IgA (1:3,000 dilution; Bethyl Laboratories, Montgomery, TX) were used as the secondary antibodies ( ).

    Techniques:

    Human splenic ILCs activate MZ B cell-helper neutrophils via GM-CSF ( a ) IHC of spleen stained for CD117 (red) and CD66 (brown). Original magnification, ×20 or ×40 (inset). ( b ) Left: qRT-PCR of CSF2 (GM-CSF) and IL8 (IL-8) mRNAs from splenic ILCs, NK cells, macrophages (Mϕ), B cells and T cells. CSF2 and IL8 data are normalized to ACTB (β-actin) mRNA and presented as relative expression (RE) compared with that of fresh splenic NK cells. Right: ELISA of GM-CSF and IL-8 from splenic ILCs and NK cells cultured for 48 h with IL-1β and IL-7. ( c ) Flow cytometry of intracellular GMCSF and IL-8 in splenic CD127 + (Lin – CD117 + ) ILCs activated with or without PMA (P) plus ionomycin (I) for 4 h. ( d ) Flow cytometry of CD69, CD11b, CD24 and CD62L on Nc cells cultured for 24 h with or without splenic ILCs in the presence or absence of control IgG1 or blocking anti-GM-CSF antibodies. Gray shading: isotype control. ( e ) Flow cytometry of viable DAPI – N c cells cultured with medium alone (Ctrl), GM-CSF, ILCs or ILC-derived conditioned medium (ILC-CM) in the presence or absence of IgG1 or anti-GM-CSF antibodies. ( f ) qRT-PCR of TNFSF13 (APRIL) mRNA from N c cells cultured for 24 h with medium alone (Ctrl), GM-CSF or ILC-CM. Results are normalized to ACTB mRNA and presented as RE compared with that of N c cells cultured with medium alone. ( g ) ELISA of IgA from splenic MZ B cells cultured for 5 d with or without N c cells, ILCs and/or ILC-CM. ( h ) ELISA of IgM, IgA and IgG from splenic MZ B cells cultured for 5 d with or without N BH cells, ILC-CM and/or N BH cells preconditioned with ILC-CM. ( i ) IFA of elastase (green) and DAPI-stained DNA (blue) in N c cells cultured for 3 h either with medium alone (Ctrl), GM-CSF, ILC-CM or LPS (original magnification, ×63) and quantification of NET-forming N c cells from nine ×10 fields from two independent experiments. Error bars, s.e.m.; * P

    Journal: Nature immunology

    Article Title: Innate lymphoid cells integrate stromal and immune signals to enhance antibody production by splenic marginal zone B cells

    doi: 10.1038/ni.2830

    Figure Lengend Snippet: Human splenic ILCs activate MZ B cell-helper neutrophils via GM-CSF ( a ) IHC of spleen stained for CD117 (red) and CD66 (brown). Original magnification, ×20 or ×40 (inset). ( b ) Left: qRT-PCR of CSF2 (GM-CSF) and IL8 (IL-8) mRNAs from splenic ILCs, NK cells, macrophages (Mϕ), B cells and T cells. CSF2 and IL8 data are normalized to ACTB (β-actin) mRNA and presented as relative expression (RE) compared with that of fresh splenic NK cells. Right: ELISA of GM-CSF and IL-8 from splenic ILCs and NK cells cultured for 48 h with IL-1β and IL-7. ( c ) Flow cytometry of intracellular GMCSF and IL-8 in splenic CD127 + (Lin – CD117 + ) ILCs activated with or without PMA (P) plus ionomycin (I) for 4 h. ( d ) Flow cytometry of CD69, CD11b, CD24 and CD62L on Nc cells cultured for 24 h with or without splenic ILCs in the presence or absence of control IgG1 or blocking anti-GM-CSF antibodies. Gray shading: isotype control. ( e ) Flow cytometry of viable DAPI – N c cells cultured with medium alone (Ctrl), GM-CSF, ILCs or ILC-derived conditioned medium (ILC-CM) in the presence or absence of IgG1 or anti-GM-CSF antibodies. ( f ) qRT-PCR of TNFSF13 (APRIL) mRNA from N c cells cultured for 24 h with medium alone (Ctrl), GM-CSF or ILC-CM. Results are normalized to ACTB mRNA and presented as RE compared with that of N c cells cultured with medium alone. ( g ) ELISA of IgA from splenic MZ B cells cultured for 5 d with or without N c cells, ILCs and/or ILC-CM. ( h ) ELISA of IgM, IgA and IgG from splenic MZ B cells cultured for 5 d with or without N BH cells, ILC-CM and/or N BH cells preconditioned with ILC-CM. ( i ) IFA of elastase (green) and DAPI-stained DNA (blue) in N c cells cultured for 3 h either with medium alone (Ctrl), GM-CSF, ILC-CM or LPS (original magnification, ×63) and quantification of NET-forming N c cells from nine ×10 fields from two independent experiments. Error bars, s.e.m.; * P

    Article Snippet: To detect mouse TNP-specific IgG3, ELISA plates coated with 5 μg/ml bovine serum albumin (BSA)-conjugated TNP (BioSearch Technologies) were sequentially incubated with mouse serum and HRP-conjugated goat polyclonal antibody A90-111P to mouse IgG3 (Bethyl Laboratories).

    Techniques: Immunohistochemistry, Staining, Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay, Cell Culture, Flow Cytometry, Cytometry, Blocking Assay, Derivative Assay, Immunofluorescence

    Mouse splenic neutrophils help plasma cells emerging from TI IgG3 responses and receive homeostatic signals from ILCs via GM-CSF ( a ) IFA of IgG3 (green), Ly6G (red) and IgM (blue) in spleens from ILC + or ILC – bone marrow chimeric mice. Original magnification, ×20. ( b ) Flow cytometric analysis of the frequency of splenic Ly6G + CD11b + neutrophils from ILC + ( n = 3) or ILC – ( n = 3) mice. ( c ) Frequency and absolute numbers of splenic Ly6G + CD11b + neutrophils from ILC + mice treated with control (Ctrl) ( n = 4) or anti-Ly6G antibodies ( n = 4). ( d , e ) Frequency and absolute numbers of splenic Lin – CD117 + CD127 + (d) as well as splenic IgG3E + IgG3I lo B cells, IgG3E hi IgG3I + plasmablasts (PBs) and IgG3E + IgG3I hi plasma cells (PCs) from ILC + mice treated as in (c). E, extracellular; I, intracellular. ( f ) ELISA of total serum IgG3 from ILC + mice treated as in (c). ( g ) qRT-PCR of Csf2 (GM-CSF) mRNA from splenic ILCs, macrophages (Mϕ), DCs, neutrophils, NK cells, T cells or B cells. Results are normalized to Gapdh (glyceraldehyde 3-phosphate dehydrogenase) mRNA and presented as relative expression (RE) compared to the expression level in NK cells. ( h ) Frequency and absolute numbers of splenic Ly6G + CD11b + neutrophils from Csf2 +/+ ( n = 8) and Cfs2 –/– ( n = 12) mice before and after transfer of B16 Cfs2 cells overexpressing GM-CSF ( n = 3). ( i ) Frequency of splenic neutrophils in ILC-sufficient Rag1 –/– Il2rg +/+ mice or ILC-insufficient Rag1 –/– Il2rg –/– mice reconstituted or not with gut ILCs from Csf2 +/+ or Cfs2 –/– mice ( n = 3). Error bars, s.e.m. (g-i), s.d. (b-e); * P

    Journal: Nature immunology

    Article Title: Innate lymphoid cells integrate stromal and immune signals to enhance antibody production by splenic marginal zone B cells

    doi: 10.1038/ni.2830

    Figure Lengend Snippet: Mouse splenic neutrophils help plasma cells emerging from TI IgG3 responses and receive homeostatic signals from ILCs via GM-CSF ( a ) IFA of IgG3 (green), Ly6G (red) and IgM (blue) in spleens from ILC + or ILC – bone marrow chimeric mice. Original magnification, ×20. ( b ) Flow cytometric analysis of the frequency of splenic Ly6G + CD11b + neutrophils from ILC + ( n = 3) or ILC – ( n = 3) mice. ( c ) Frequency and absolute numbers of splenic Ly6G + CD11b + neutrophils from ILC + mice treated with control (Ctrl) ( n = 4) or anti-Ly6G antibodies ( n = 4). ( d , e ) Frequency and absolute numbers of splenic Lin – CD117 + CD127 + (d) as well as splenic IgG3E + IgG3I lo B cells, IgG3E hi IgG3I + plasmablasts (PBs) and IgG3E + IgG3I hi plasma cells (PCs) from ILC + mice treated as in (c). E, extracellular; I, intracellular. ( f ) ELISA of total serum IgG3 from ILC + mice treated as in (c). ( g ) qRT-PCR of Csf2 (GM-CSF) mRNA from splenic ILCs, macrophages (Mϕ), DCs, neutrophils, NK cells, T cells or B cells. Results are normalized to Gapdh (glyceraldehyde 3-phosphate dehydrogenase) mRNA and presented as relative expression (RE) compared to the expression level in NK cells. ( h ) Frequency and absolute numbers of splenic Ly6G + CD11b + neutrophils from Csf2 +/+ ( n = 8) and Cfs2 –/– ( n = 12) mice before and after transfer of B16 Cfs2 cells overexpressing GM-CSF ( n = 3). ( i ) Frequency of splenic neutrophils in ILC-sufficient Rag1 –/– Il2rg +/+ mice or ILC-insufficient Rag1 –/– Il2rg –/– mice reconstituted or not with gut ILCs from Csf2 +/+ or Cfs2 –/– mice ( n = 3). Error bars, s.e.m. (g-i), s.d. (b-e); * P

    Article Snippet: To detect mouse TNP-specific IgG3, ELISA plates coated with 5 μg/ml bovine serum albumin (BSA)-conjugated TNP (BioSearch Technologies) were sequentially incubated with mouse serum and HRP-conjugated goat polyclonal antibody A90-111P to mouse IgG3 (Bethyl Laboratories).

    Techniques: Immunofluorescence, Mouse Assay, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Expressing

    Mouse splenic ILCs help plasmablasts and plasma cells emerging from TI IgG3 responses ( a ) Flow cytometric analysis of frequency and absolute numbers of splenic Lin – CD117 + CD127 + ILCs from Thy-1-disparate chimeric Rag1 –/– mice treated with control (ctrl) ( n = 7) or anti-Thy.1.2 antibodies ( n = 7). ( b ) Frequency and absolute numbers of splenic IgG3E + IgG3I lo B cells, IgG3E hi IgG3I + plasmablasts (PBs) and IgG3E – IgG3I hi plasma cells (PCs) from Ctrl or ILC-depleted mice obtained as in (a). E, extracellular; I, intracellular. ( c,d ) ELISA of pre-immune total (c) and PC-reactive (d) serum IgG3 from Ctrl or ILC-depleted mice obtained as in (a). ( e , f ) Frequency and absolute numbers of splenic Lin – CD117 + CD127 + ILCs (e) and IgG3-expressing splenic B cells, PBs and PCs (f) from ILC + ( n = 3) or ILC – ( n = 3) bone marrow chimeric mice. ( g ) Pre-immune total serum IgG3 from ILC + ( n = 4) and ILC – ( n = 3) mice, age-matched Rorc +/+ mice ( n = 10), and Rorc –/– mice ( n = 5). Error bars, s.d.; * P

    Journal: Nature immunology

    Article Title: Innate lymphoid cells integrate stromal and immune signals to enhance antibody production by splenic marginal zone B cells

    doi: 10.1038/ni.2830

    Figure Lengend Snippet: Mouse splenic ILCs help plasmablasts and plasma cells emerging from TI IgG3 responses ( a ) Flow cytometric analysis of frequency and absolute numbers of splenic Lin – CD117 + CD127 + ILCs from Thy-1-disparate chimeric Rag1 –/– mice treated with control (ctrl) ( n = 7) or anti-Thy.1.2 antibodies ( n = 7). ( b ) Frequency and absolute numbers of splenic IgG3E + IgG3I lo B cells, IgG3E hi IgG3I + plasmablasts (PBs) and IgG3E – IgG3I hi plasma cells (PCs) from Ctrl or ILC-depleted mice obtained as in (a). E, extracellular; I, intracellular. ( c,d ) ELISA of pre-immune total (c) and PC-reactive (d) serum IgG3 from Ctrl or ILC-depleted mice obtained as in (a). ( e , f ) Frequency and absolute numbers of splenic Lin – CD117 + CD127 + ILCs (e) and IgG3-expressing splenic B cells, PBs and PCs (f) from ILC + ( n = 3) or ILC – ( n = 3) bone marrow chimeric mice. ( g ) Pre-immune total serum IgG3 from ILC + ( n = 4) and ILC – ( n = 3) mice, age-matched Rorc +/+ mice ( n = 10), and Rorc –/– mice ( n = 5). Error bars, s.d.; * P

    Article Snippet: To detect mouse TNP-specific IgG3, ELISA plates coated with 5 μg/ml bovine serum albumin (BSA)-conjugated TNP (BioSearch Technologies) were sequentially incubated with mouse serum and HRP-conjugated goat polyclonal antibody A90-111P to mouse IgG3 (Bethyl Laboratories).

    Techniques: Flow Cytometry, Mouse Assay, Enzyme-linked Immunosorbent Assay, Expressing

    Human splenic ILCs express the MZ B cell-helper factors BAFF, CD40L and DLL1 and activate MZ B cells in cooperation with MRCs ( a) IHC of spleen stained for RORγt (brown) and CD20 (purple). Arrowheads point to RORγt + cells. Original magnification, ×40. ( b ) qRT-PCR of TNFSF13B (BAFF), TNFSF13 (APRIL), CD40LG (CD40L) and DLL1 (DLL1) mRNAs from splenic or tonsillar ILCs and NK cells and from splenic macrophages (Mϕ), B cells and T cells. Results are normalized to ACTB (β-actin) mRNA and presented as relative expression (RE) compared with that of fresh splenic NK cells. ( c) Flow cytometry of BAFF, APRIL, CD40L and DLL1 on splenic ILCs exposed to IL-1β plus IL-7 for 72 h. Gray shading, negative control. ( d ) ELISA of soluble BAFF and APRIL from splenic ILCs and NK cells cultured as in (c) as well as Mϕ. ( e ) Flow cytometric analysis of the frequency of viable Annexin-V – propidium iodide (PI) – and divided carboxyfluorescein diacetate succinimidyl ester (CFSE) low splenic MZ (blue bars) and FO (pink bars) B cells after incubation with medium alone (ctrl) or ILCs for 5 d. ( f ) ELISA of IgM, IgG and IgA from splenic MZ B cells incubated for 5 d with medium alone (Ctrl), ILCs, MRCs and/or CpG. ( g ) Frequency of CD27 hi CD38 hi plasmablasts in splenic MZ B cells cultured for 5 d as in (f). Error bars, s.e.m.; * P

    Journal: Nature immunology

    Article Title: Innate lymphoid cells integrate stromal and immune signals to enhance antibody production by splenic marginal zone B cells

    doi: 10.1038/ni.2830

    Figure Lengend Snippet: Human splenic ILCs express the MZ B cell-helper factors BAFF, CD40L and DLL1 and activate MZ B cells in cooperation with MRCs ( a) IHC of spleen stained for RORγt (brown) and CD20 (purple). Arrowheads point to RORγt + cells. Original magnification, ×40. ( b ) qRT-PCR of TNFSF13B (BAFF), TNFSF13 (APRIL), CD40LG (CD40L) and DLL1 (DLL1) mRNAs from splenic or tonsillar ILCs and NK cells and from splenic macrophages (Mϕ), B cells and T cells. Results are normalized to ACTB (β-actin) mRNA and presented as relative expression (RE) compared with that of fresh splenic NK cells. ( c) Flow cytometry of BAFF, APRIL, CD40L and DLL1 on splenic ILCs exposed to IL-1β plus IL-7 for 72 h. Gray shading, negative control. ( d ) ELISA of soluble BAFF and APRIL from splenic ILCs and NK cells cultured as in (c) as well as Mϕ. ( e ) Flow cytometric analysis of the frequency of viable Annexin-V – propidium iodide (PI) – and divided carboxyfluorescein diacetate succinimidyl ester (CFSE) low splenic MZ (blue bars) and FO (pink bars) B cells after incubation with medium alone (ctrl) or ILCs for 5 d. ( f ) ELISA of IgM, IgG and IgA from splenic MZ B cells incubated for 5 d with medium alone (Ctrl), ILCs, MRCs and/or CpG. ( g ) Frequency of CD27 hi CD38 hi plasmablasts in splenic MZ B cells cultured for 5 d as in (f). Error bars, s.e.m.; * P

    Article Snippet: To detect mouse TNP-specific IgG3, ELISA plates coated with 5 μg/ml bovine serum albumin (BSA)-conjugated TNP (BioSearch Technologies) were sequentially incubated with mouse serum and HRP-conjugated goat polyclonal antibody A90-111P to mouse IgG3 (Bethyl Laboratories).

    Techniques: Immunohistochemistry, Staining, Quantitative RT-PCR, Expressing, Flow Cytometry, Cytometry, Negative Control, Enzyme-linked Immunosorbent Assay, Cell Culture, Incubation

    Mouse splenic ILCs express the plasma cell-helper factors APRIL and DLL1 and enhance TI IgG3 responses ( a ) Flow cytometric analysis of frequency and absolute numbers of splenic Lin – CD117 + CD127 + ILCs from Rorc +/+ ( n = 3) and Rorc –/– mice ( n = 3). ( b ) qRT-PCR of Rorc (RORγt) Il22 (IL-22) Tnf (TNF), Lta (LT-α), Tnfsf13 (APRIL), Tnfsf13b (BAFF), CD40lg (CD40L) and Dll1 (DLL1) mRNAs from splenic ILCs, macrophages (Mϕ), DCs, neutrophils, NK cells, T cells or B cells. Results are normalized to Gapdh (glyceraldehyde 3-phosphate dehydrogenase) mRNA and presented as relative expression (RE) compared to the expression level in NK cells. ( c ) Flow cytometric analysis of frequency and absolute numbers of splenic IgG3E + IgG3I lo B cells, IgG3E hi IgG3I + plasmablasts (PBs) and IgG3E + IgG3I hi plasma cells (PCs) from Rorc +/+ ( n = 3) and Rorc –/– mice. E, extracellular; I, intracellular. ( d ) IFA of IgM (green), IgG3 (red) and MOMA-1 (blue) in spleens from Rorc +/+ and Rorc –/– mice. Original magnification, ×20. ( e,f ) ELISA of total (e) and PC-reactive (f) serum IgG3 from Rorc +/+ ( n = 6-7) and Rorc –/– ( n = 7-8) mice. ( g ) Frequency of IgG3-expressing B cells, PBs and PCs from spleens of Rorc +/– Cd3e –/– and Rorc –/– Cd3e –/– mice. ( h ) Serum IgG3 from Rorc +/– Cd3e –/– ( n = 9) and Rorc –/– Cd3e –/– ( n = 6) mice. Error bars, s.e.m. (b), s.d. (a,c); * P

    Journal: Nature immunology

    Article Title: Innate lymphoid cells integrate stromal and immune signals to enhance antibody production by splenic marginal zone B cells

    doi: 10.1038/ni.2830

    Figure Lengend Snippet: Mouse splenic ILCs express the plasma cell-helper factors APRIL and DLL1 and enhance TI IgG3 responses ( a ) Flow cytometric analysis of frequency and absolute numbers of splenic Lin – CD117 + CD127 + ILCs from Rorc +/+ ( n = 3) and Rorc –/– mice ( n = 3). ( b ) qRT-PCR of Rorc (RORγt) Il22 (IL-22) Tnf (TNF), Lta (LT-α), Tnfsf13 (APRIL), Tnfsf13b (BAFF), CD40lg (CD40L) and Dll1 (DLL1) mRNAs from splenic ILCs, macrophages (Mϕ), DCs, neutrophils, NK cells, T cells or B cells. Results are normalized to Gapdh (glyceraldehyde 3-phosphate dehydrogenase) mRNA and presented as relative expression (RE) compared to the expression level in NK cells. ( c ) Flow cytometric analysis of frequency and absolute numbers of splenic IgG3E + IgG3I lo B cells, IgG3E hi IgG3I + plasmablasts (PBs) and IgG3E + IgG3I hi plasma cells (PCs) from Rorc +/+ ( n = 3) and Rorc –/– mice. E, extracellular; I, intracellular. ( d ) IFA of IgM (green), IgG3 (red) and MOMA-1 (blue) in spleens from Rorc +/+ and Rorc –/– mice. Original magnification, ×20. ( e,f ) ELISA of total (e) and PC-reactive (f) serum IgG3 from Rorc +/+ ( n = 6-7) and Rorc –/– ( n = 7-8) mice. ( g ) Frequency of IgG3-expressing B cells, PBs and PCs from spleens of Rorc +/– Cd3e –/– and Rorc –/– Cd3e –/– mice. ( h ) Serum IgG3 from Rorc +/– Cd3e –/– ( n = 9) and Rorc –/– Cd3e –/– ( n = 6) mice. Error bars, s.e.m. (b), s.d. (a,c); * P

    Article Snippet: To detect mouse TNP-specific IgG3, ELISA plates coated with 5 μg/ml bovine serum albumin (BSA)-conjugated TNP (BioSearch Technologies) were sequentially incubated with mouse serum and HRP-conjugated goat polyclonal antibody A90-111P to mouse IgG3 (Bethyl Laboratories).

    Techniques: Flow Cytometry, Mouse Assay, Quantitative RT-PCR, Expressing, Immunofluorescence, Enzyme-linked Immunosorbent Assay

    Transitions of the fluorescence intensity in the well with the film-stack reaction fields with the micro-pillars array having different dimensions in relation in relation to HRP-IgA incubation time. All fluorescence intensities are the same with Figure 7 . Error bars are the maximum and minimum intensities.

    Journal: Sensors (Basel, Switzerland)

    Article Title: Rapid ELISA Using a Film-Stack Reaction Field with Micropillar Arrays

    doi: 10.3390/s17071608

    Figure Lengend Snippet: Transitions of the fluorescence intensity in the well with the film-stack reaction fields with the micro-pillars array having different dimensions in relation in relation to HRP-IgA incubation time. All fluorescence intensities are the same with Figure 7 . Error bars are the maximum and minimum intensities.

    Article Snippet: Furthermore, for the incubation of horseradish peroxidase conjugated goat human IgA antibody (HRP-IgA, Bethyl Laboratories, Inc., Montgomery, TX, USA) in each well, 100 µL of 0.01% HRP-IgA diluted in TBS-BSA was added to each well.

    Techniques: Fluorescence, Incubation

    Fluorescence intensities of IgA ELISA in relation to HRP-IgA incubation time for wells without and with the film-stack reactions with the micro-pillars array having different dimensions. The trial number of experiments is three. Error bars are the maximum and minimum intensities.

    Journal: Sensors (Basel, Switzerland)

    Article Title: Rapid ELISA Using a Film-Stack Reaction Field with Micropillar Arrays

    doi: 10.3390/s17071608

    Figure Lengend Snippet: Fluorescence intensities of IgA ELISA in relation to HRP-IgA incubation time for wells without and with the film-stack reactions with the micro-pillars array having different dimensions. The trial number of experiments is three. Error bars are the maximum and minimum intensities.

    Article Snippet: Furthermore, for the incubation of horseradish peroxidase conjugated goat human IgA antibody (HRP-IgA, Bethyl Laboratories, Inc., Montgomery, TX, USA) in each well, 100 µL of 0.01% HRP-IgA diluted in TBS-BSA was added to each well.

    Techniques: Fluorescence, Enzyme-linked Immunosorbent Assay, Incubation

    Comparison of IgG, IgM and IgA antibody against three antigens of NTHi in the serum samples of children at their acute visit of AOM in 32 otitis prone (black bar), 27 AOMTF (gray bar) and 26 Non-otitis prone (white bar) children.

    Journal: Vaccine

    Article Title: Serum Antibody Response to Three Non-typeable Haemophilus influenzae Outer Membrane Proteins During Acute Otitis Media and Nasopharyngeal Colonization in Otitis Prone and Non-Otitis Prone Children

    doi: 10.1016/j.vaccine.2010.11.055

    Figure Lengend Snippet: Comparison of IgG, IgM and IgA antibody against three antigens of NTHi in the serum samples of children at their acute visit of AOM in 32 otitis prone (black bar), 27 AOMTF (gray bar) and 26 Non-otitis prone (white bar) children.

    Article Snippet: The mixture was incubated at room temperature for 1 hr followed by the addition of affinity purified goat anti-human IgG, IgM or IgA antibody conjugated to horseradish-peroxidase (Bethyl Laboratories, Inc, Montgomery, TX) as a secondary antibody.

    Techniques: