Structured Review

Abcam hrp conjugated secondary antibodies
Depletion of Anti-AAV9 Antibodies from an IVIG Solution by Immunoadsorption with AAV9 Sepharose Beads (A) Schematic depiction of immunoadsorption of anti-AAV9 antibodies. To deplete anti-AAV9 antibodies from a solution with human IVIG (intravenous immunoglobulin), was incubated the solution with Sepharose beads with covalently coupled AAV9 virions (AAV9-beads). (B) After incubation with IVIG solution, the beads were washed with PBS and the anti-AAV antibodies were eluted from the AAV9-beads by incubation with a low pH buffer. After elution of the anti-AAV antibodies, the beads were washed with PBS. The binding/elution procedure was repeated twice. Anti-AAV9 antibodies in the eluates were detected by Western blot with neutralized eluates as a primary antibody. The AAV9 capsid proteins (VP1, VP2, and VP3) were detected with an <t>HRP-anti-human-IgG</t> secondary antibody and ECL. The same matrix was reused three times without evidence of loss of binding capacity. (C) As in (B), but with BSA-beads. (D) Western blot experiment showing the presence of immunoglobulin heavy chain (HC) and light chain (LC) in the AAV9-beads eluates. (E) The total amount of antibodies remained unchanged after the incubation of IVIG with AAV9-beads, but not with protein G-beads.
Hrp Conjugated Secondary Antibodies, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 158 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hrp conjugated secondary antibodies/product/Abcam
Average 99 stars, based on 158 article reviews
Price from $9.99 to $1999.99
hrp conjugated secondary antibodies - by Bioz Stars, 2020-07
99/100 stars

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1) Product Images from "Successful Transduction with AAV Vectors after Selective Depletion of Anti-AAV Antibodies by Immunoadsorption"

Article Title: Successful Transduction with AAV Vectors after Selective Depletion of Anti-AAV Antibodies by Immunoadsorption

Journal: Molecular Therapy. Methods & Clinical Development

doi: 10.1016/j.omtm.2020.01.004

Depletion of Anti-AAV9 Antibodies from an IVIG Solution by Immunoadsorption with AAV9 Sepharose Beads (A) Schematic depiction of immunoadsorption of anti-AAV9 antibodies. To deplete anti-AAV9 antibodies from a solution with human IVIG (intravenous immunoglobulin), was incubated the solution with Sepharose beads with covalently coupled AAV9 virions (AAV9-beads). (B) After incubation with IVIG solution, the beads were washed with PBS and the anti-AAV antibodies were eluted from the AAV9-beads by incubation with a low pH buffer. After elution of the anti-AAV antibodies, the beads were washed with PBS. The binding/elution procedure was repeated twice. Anti-AAV9 antibodies in the eluates were detected by Western blot with neutralized eluates as a primary antibody. The AAV9 capsid proteins (VP1, VP2, and VP3) were detected with an HRP-anti-human-IgG secondary antibody and ECL. The same matrix was reused three times without evidence of loss of binding capacity. (C) As in (B), but with BSA-beads. (D) Western blot experiment showing the presence of immunoglobulin heavy chain (HC) and light chain (LC) in the AAV9-beads eluates. (E) The total amount of antibodies remained unchanged after the incubation of IVIG with AAV9-beads, but not with protein G-beads.
Figure Legend Snippet: Depletion of Anti-AAV9 Antibodies from an IVIG Solution by Immunoadsorption with AAV9 Sepharose Beads (A) Schematic depiction of immunoadsorption of anti-AAV9 antibodies. To deplete anti-AAV9 antibodies from a solution with human IVIG (intravenous immunoglobulin), was incubated the solution with Sepharose beads with covalently coupled AAV9 virions (AAV9-beads). (B) After incubation with IVIG solution, the beads were washed with PBS and the anti-AAV antibodies were eluted from the AAV9-beads by incubation with a low pH buffer. After elution of the anti-AAV antibodies, the beads were washed with PBS. The binding/elution procedure was repeated twice. Anti-AAV9 antibodies in the eluates were detected by Western blot with neutralized eluates as a primary antibody. The AAV9 capsid proteins (VP1, VP2, and VP3) were detected with an HRP-anti-human-IgG secondary antibody and ECL. The same matrix was reused three times without evidence of loss of binding capacity. (C) As in (B), but with BSA-beads. (D) Western blot experiment showing the presence of immunoglobulin heavy chain (HC) and light chain (LC) in the AAV9-beads eluates. (E) The total amount of antibodies remained unchanged after the incubation of IVIG with AAV9-beads, but not with protein G-beads.

Techniques Used: Incubation, Binding Assay, Western Blot

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Western Blot:

Article Title: CD36 and Fyn Kinase Mediate Malaria-Induced Lung Endothelial Barrier Dysfunction in Mice Infected with Plasmodium berghei
Article Snippet: .. Equal protein loading was confirmed by stripping the membranes (Restore Western Blot Stripping buffer, Thermo Scientific) and reprobing with HRP-conjugated anti-Actin (1∶1,000, Abcam) or anti-GAPDH (1∶25,000) antibodies. .. The blots were developed with regular or enhanced chemiluminescence (ECL or ECLplus; Amersham) and exposed to X-ray film (Kodak BioMax MR).

Stripping Membranes:

Article Title: CD36 and Fyn Kinase Mediate Malaria-Induced Lung Endothelial Barrier Dysfunction in Mice Infected with Plasmodium berghei
Article Snippet: .. Equal protein loading was confirmed by stripping the membranes (Restore Western Blot Stripping buffer, Thermo Scientific) and reprobing with HRP-conjugated anti-Actin (1∶1,000, Abcam) or anti-GAPDH (1∶25,000) antibodies. .. The blots were developed with regular or enhanced chemiluminescence (ECL or ECLplus; Amersham) and exposed to X-ray film (Kodak BioMax MR).

other:

Article Title: Size and Targeting to PECAM vs ICAM Control Endothelial Delivery, Internalization and Protective Effect of Multimolecular SOD Conjugates
Article Snippet: ; anti-actin antibody HRP-conjugated was from Abcam (Cambridge, MA).

Article Title: PKCδ regulates integrin αVβ3 expression and transformed growth of K-ras dependent lung cancer cells
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Article Title: Key Residues and Phosphate Release Routes in the Saccharomyces cerevisiae Pho84 Transceptor
Article Snippet: Anti-actin-HRP antibodies were obtained from Abcam (UK).

Stripping:

Article Title: CD36 and Fyn Kinase Mediate Malaria-Induced Lung Endothelial Barrier Dysfunction in Mice Infected with Plasmodium berghei
Article Snippet: .. Equal protein loading was confirmed by stripping the membranes (Restore Western Blot Stripping buffer, Thermo Scientific) and reprobing with HRP-conjugated anti-Actin (1∶1,000, Abcam) or anti-GAPDH (1∶25,000) antibodies. .. The blots were developed with regular or enhanced chemiluminescence (ECL or ECLplus; Amersham) and exposed to X-ray film (Kodak BioMax MR).

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    Abcam peroxidase conjugated anti mouse secondary antibody
    Effects of resveratrol on NIS protein expression in rat thyroid glands. Male Sprague-Dawley rats were treated with the control vehicle (Control, n = 4) or with 50 mg/kg/day resveratrol i.p. (Resv, n = 4), for 14 days. On day 15 th , the animals were sacrificed and their thyroids were removed. Immunofluorescence analysis was performed using a <t>mouse</t> monoclonal <t>anti-NIS</t> antibody and an anti-mouse <t>fluorescein-conjugated</t> <t>secondary</t> antibody, Alexa Fluor 488, (green). Po-Pro-3 iodide was used to stain the nuclei (red). The negative control was performed using a mouse IgG preparations instead of the primary antibody (data not shown). The slides were visualized under a Zeiss LSM S10 confocal microscope with a x40 immersion lens. Representative data from four experiments are showed.
    Peroxidase Conjugated Anti Mouse Secondary Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam hrp conjugated goat anti rabbit igg
    Binding ELISA demonstrating the autoantibody binding to Met5A mesothelial cells due to the presence of MCAA in serum from human patients exposed to LA fibers. Sera were pooled from MCAA positive samples (MCAA+) and MCAA-positive samples cleared of all <t>IgG</t> (Cleared). Human mesothelial cells were plated in 96-well plates and then stained for MCAA binding using the pooled serum as the primary antibody and anti-human IgG - <t>HRP</t> for the secondary antibody. N= 3 samples per treatment group. Data are mean absorbance at 450 nm. *=p
    Hrp Conjugated Goat Anti Rabbit Igg, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Abcam horse radish peroxidase hrp conjugated rabbit anti mouse secondary antibody
    Evaluation of the binding selectivity of the selected phage clones by cell ELISA Cells were seeded onto 96-well cell culture plates overnight (1×10 4 cells/well). 10 9 phage particles were added to each well. Phage binding to cells was detected through adding mouse <t>anti-M13</t> phage antibody and <t>HRP-conjugated</t> rabbit anti-mouse secondary antibody. OD was obtained after blocking the reaction. The selectivity values for phage binding was calculated by the formula mentioned in the text and were 5.1, 2.2, 2.9, 2.5, 1.4, and 1.3 for P1, P2, P3, P4, P5, and P6, respectively. P1 is indicated to be the strongest binder and binds more effectively than all phage clones to A549 cells
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    Effects of resveratrol on NIS protein expression in rat thyroid glands. Male Sprague-Dawley rats were treated with the control vehicle (Control, n = 4) or with 50 mg/kg/day resveratrol i.p. (Resv, n = 4), for 14 days. On day 15 th , the animals were sacrificed and their thyroids were removed. Immunofluorescence analysis was performed using a mouse monoclonal anti-NIS antibody and an anti-mouse fluorescein-conjugated secondary antibody, Alexa Fluor 488, (green). Po-Pro-3 iodide was used to stain the nuclei (red). The negative control was performed using a mouse IgG preparations instead of the primary antibody (data not shown). The slides were visualized under a Zeiss LSM S10 confocal microscope with a x40 immersion lens. Representative data from four experiments are showed.

    Journal: PLoS ONE

    Article Title: Resveratrol Inhibits Sodium/Iodide Symporter Gene Expression and Function in Rat Thyroid Cells

    doi: 10.1371/journal.pone.0107936

    Figure Lengend Snippet: Effects of resveratrol on NIS protein expression in rat thyroid glands. Male Sprague-Dawley rats were treated with the control vehicle (Control, n = 4) or with 50 mg/kg/day resveratrol i.p. (Resv, n = 4), for 14 days. On day 15 th , the animals were sacrificed and their thyroids were removed. Immunofluorescence analysis was performed using a mouse monoclonal anti-NIS antibody and an anti-mouse fluorescein-conjugated secondary antibody, Alexa Fluor 488, (green). Po-Pro-3 iodide was used to stain the nuclei (red). The negative control was performed using a mouse IgG preparations instead of the primary antibody (data not shown). The slides were visualized under a Zeiss LSM S10 confocal microscope with a x40 immersion lens. Representative data from four experiments are showed.

    Article Snippet: The membranes were subsequently washed and incubated with a horseradish peroxidase-conjugated anti-mouse secondary antibody (ab6789, Abcam, Cambridge, UK), following the manufacturer instructions.

    Techniques: Expressing, Immunofluorescence, Staining, Negative Control, Microscopy

    Binding ELISA demonstrating the autoantibody binding to Met5A mesothelial cells due to the presence of MCAA in serum from human patients exposed to LA fibers. Sera were pooled from MCAA positive samples (MCAA+) and MCAA-positive samples cleared of all IgG (Cleared). Human mesothelial cells were plated in 96-well plates and then stained for MCAA binding using the pooled serum as the primary antibody and anti-human IgG - HRP for the secondary antibody. N= 3 samples per treatment group. Data are mean absorbance at 450 nm. *=p

    Journal: Inhalation toxicology

    Article Title: Mesothelial Cell Autoantibodies Upregulate Transcription Factors Associated with Fibrosis

    doi: 10.1080/08958378.2016.1271841

    Figure Lengend Snippet: Binding ELISA demonstrating the autoantibody binding to Met5A mesothelial cells due to the presence of MCAA in serum from human patients exposed to LA fibers. Sera were pooled from MCAA positive samples (MCAA+) and MCAA-positive samples cleared of all IgG (Cleared). Human mesothelial cells were plated in 96-well plates and then stained for MCAA binding using the pooled serum as the primary antibody and anti-human IgG - HRP for the secondary antibody. N= 3 samples per treatment group. Data are mean absorbance at 450 nm. *=p

    Article Snippet: The secondary antibody used was HRP-conjugated goat anti-rabbit IgG (ABCAM) diluted 1:1000 with 3%BSA/PBS.

    Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Staining

    Evaluation of the binding selectivity of the selected phage clones by cell ELISA Cells were seeded onto 96-well cell culture plates overnight (1×10 4 cells/well). 10 9 phage particles were added to each well. Phage binding to cells was detected through adding mouse anti-M13 phage antibody and HRP-conjugated rabbit anti-mouse secondary antibody. OD was obtained after blocking the reaction. The selectivity values for phage binding was calculated by the formula mentioned in the text and were 5.1, 2.2, 2.9, 2.5, 1.4, and 1.3 for P1, P2, P3, P4, P5, and P6, respectively. P1 is indicated to be the strongest binder and binds more effectively than all phage clones to A549 cells

    Journal: Iranian Journal of Pharmaceutical Research : IJPR

    Article Title: Identification of a Novel Tumor-Binding Peptide for Lung Cancer Through in-vitro Panning

    doi:

    Figure Lengend Snippet: Evaluation of the binding selectivity of the selected phage clones by cell ELISA Cells were seeded onto 96-well cell culture plates overnight (1×10 4 cells/well). 10 9 phage particles were added to each well. Phage binding to cells was detected through adding mouse anti-M13 phage antibody and HRP-conjugated rabbit anti-mouse secondary antibody. OD was obtained after blocking the reaction. The selectivity values for phage binding was calculated by the formula mentioned in the text and were 5.1, 2.2, 2.9, 2.5, 1.4, and 1.3 for P1, P2, P3, P4, P5, and P6, respectively. P1 is indicated to be the strongest binder and binds more effectively than all phage clones to A549 cells

    Article Snippet: Mouse anti-M13 phage antibody and horse radish peroxidase (HRP)-conjugated rabbit anti-mouse secondary antibody were purchased from Abcam Inc (MA, USA).

    Techniques: Binding Assay, Clone Assay, Enzyme-linked Immunosorbent Assay, Cell Culture, Blocking Assay

    Evaluation of the binding selectivity of the selected phage clones by cell ELISA Cells were seeded onto 96-well cell culture plates overnight (1×10 4 cells/well). 10 9 phage particles were added to each well. Phage binding to cells was detected through adding mouse anti-M13 phage antibody and HRP-conjugated rabbit anti-mouse secondary antibody. OD was obtained after blocking the reaction. The selectivity values for phage binding was calculated by the formula mentioned in the text and were 5.1, 2.2, 2.9, 2.5, 1.4, and 1.3 for P1, P2, P3, P4, P5, and P6, respectively. P1 is indicated to be the strongest binder and binds more effectively than all phage clones to A549 cells

    Journal: Iranian Journal of Pharmaceutical Research : IJPR

    Article Title: Identification of a Novel Tumor-Binding Peptide for Lung Cancer Through in-vitro Panning

    doi:

    Figure Lengend Snippet: Evaluation of the binding selectivity of the selected phage clones by cell ELISA Cells were seeded onto 96-well cell culture plates overnight (1×10 4 cells/well). 10 9 phage particles were added to each well. Phage binding to cells was detected through adding mouse anti-M13 phage antibody and HRP-conjugated rabbit anti-mouse secondary antibody. OD was obtained after blocking the reaction. The selectivity values for phage binding was calculated by the formula mentioned in the text and were 5.1, 2.2, 2.9, 2.5, 1.4, and 1.3 for P1, P2, P3, P4, P5, and P6, respectively. P1 is indicated to be the strongest binder and binds more effectively than all phage clones to A549 cells

    Article Snippet: Mouse anti-M13 phage antibody and horse radish peroxidase (HRP)-conjugated rabbit anti-mouse secondary antibody were purchased from Abcam Inc (MA, USA).

    Techniques: Binding Assay, Clone Assay, Enzyme-linked Immunosorbent Assay, Cell Culture, Blocking Assay