Structured Review

Jackson Immuno hrp conjugated goat anti mouse
JK-6L cells show enhanced protein accumulation in the presence of bortezomib and verapamil. (A) Western blot analysis of detergent-soluble and detergent-insoluble fractions of JK-6L cells treated with 10 nM bortezomib and/or 70 µ M verapamil for 16 hours. Immunoblot analysiswas done using an anti-ubiquitin antibody. To detect free ubiquitin, a denser gel was used. One representative immunoblot of two independent experiments is shown. (B) Western blot analysis of total cell lysates fromJK-6L cells treated with 10 nM bortezomib and/or 70 µMverapamil for 16 hours. Immunoblot analysis was done using <t>HRP-conjugated</t> <t>anti-IgG</t> antibody. One representative immunoblot of two independent experiments is shown. (C) IgG concentration in the supernatant of JK-6L cells treated with 10 nM bortezomib and/or 70 µ M verapamil for 16 hourswas determined by ELISA. The absorbance (OD)wasmeasured using a spectrophotometer. Mean values and SDwere calculated fromhexaplicates. Data represent one of two independently done experiments. Student's t -test for unpaired heteroscedastic samples was used for statistical analysis. # P
Hrp Conjugated Goat Anti Mouse, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 92/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hrp conjugated goat anti mouse/product/Jackson Immuno
Average 92 stars, based on 17 article reviews
Price from $9.99 to $1999.99
hrp conjugated goat anti mouse - by Bioz Stars, 2020-09
92/100 stars

Images

1) Product Images from "Calcium Channel Blocker Verapamil Enhances Endoplasmic Reticulum Stress and Cell Death Induced by Proteasome Inhibition in Myeloma Cells 1Calcium Channel Blocker Verapamil Enhances Endoplasmic Reticulum Stress and Cell Death Induced by Proteasome Inhibition in Myeloma Cells 1 2"

Article Title: Calcium Channel Blocker Verapamil Enhances Endoplasmic Reticulum Stress and Cell Death Induced by Proteasome Inhibition in Myeloma Cells 1Calcium Channel Blocker Verapamil Enhances Endoplasmic Reticulum Stress and Cell Death Induced by Proteasome Inhibition in Myeloma Cells 1 2

Journal: Neoplasia (New York, N.Y.)

doi:

JK-6L cells show enhanced protein accumulation in the presence of bortezomib and verapamil. (A) Western blot analysis of detergent-soluble and detergent-insoluble fractions of JK-6L cells treated with 10 nM bortezomib and/or 70 µ M verapamil for 16 hours. Immunoblot analysiswas done using an anti-ubiquitin antibody. To detect free ubiquitin, a denser gel was used. One representative immunoblot of two independent experiments is shown. (B) Western blot analysis of total cell lysates fromJK-6L cells treated with 10 nM bortezomib and/or 70 µMverapamil for 16 hours. Immunoblot analysis was done using HRP-conjugated anti-IgG antibody. One representative immunoblot of two independent experiments is shown. (C) IgG concentration in the supernatant of JK-6L cells treated with 10 nM bortezomib and/or 70 µ M verapamil for 16 hourswas determined by ELISA. The absorbance (OD)wasmeasured using a spectrophotometer. Mean values and SDwere calculated fromhexaplicates. Data represent one of two independently done experiments. Student's t -test for unpaired heteroscedastic samples was used for statistical analysis. # P
Figure Legend Snippet: JK-6L cells show enhanced protein accumulation in the presence of bortezomib and verapamil. (A) Western blot analysis of detergent-soluble and detergent-insoluble fractions of JK-6L cells treated with 10 nM bortezomib and/or 70 µ M verapamil for 16 hours. Immunoblot analysiswas done using an anti-ubiquitin antibody. To detect free ubiquitin, a denser gel was used. One representative immunoblot of two independent experiments is shown. (B) Western blot analysis of total cell lysates fromJK-6L cells treated with 10 nM bortezomib and/or 70 µMverapamil for 16 hours. Immunoblot analysis was done using HRP-conjugated anti-IgG antibody. One representative immunoblot of two independent experiments is shown. (C) IgG concentration in the supernatant of JK-6L cells treated with 10 nM bortezomib and/or 70 µ M verapamil for 16 hourswas determined by ELISA. The absorbance (OD)wasmeasured using a spectrophotometer. Mean values and SDwere calculated fromhexaplicates. Data represent one of two independently done experiments. Student's t -test for unpaired heteroscedastic samples was used for statistical analysis. # P

Techniques Used: Western Blot, Concentration Assay, Enzyme-linked Immunosorbent Assay, Spectrophotometry

Related Articles

Western Blot:

Article Title: Mitotic Modulation of Translation Elongation Factor 1 Leads to Hindered tRNA Delivery to Ribosomes *
Article Snippet: .. HRP-conjugated goat anti-mouse and anti-rabbit secondary antibodies for Western blotting were from Jackson ImmunoResearch Laboratories, Inc. .. Secondary antibodies for immunofluorescence were all multilabeling grade from Jackson ImmunoResearch Laboratories, Inc.

Incubation:

Article Title: Mechanisms Underlying the Antiproliferative and Prodifferentiative Effects of Psoralen on Adult Neural Stem Cells via DNA Microarray
Article Snippet: .. The membranes were blocked with 5% (w/v) skim milk in phosphate-buffered saline containing 0.1% Tween 20 and blotted with antibodies for glial fibrillary acidic protein (GFAP) (1 : 500; Chemicon, USA) and β -tubulin III (TuJ1) (1 : 200, Chemicon, USA) followed by incubation with appropriate sets of secondary HRP-conjugated goat anti-mouse or rabbit antibodies (1 : 5000; Jackson ImmunoResearch, USA). .. Immunoreactive bands were visualized with ECL reagents (Biyotime, Shanghai, China).

Article Title: A complex of three related membrane proteins is conserved on malarial merozoites
Article Snippet: .. After extensive washing, blots were incubated with HRP-conjugated goat anti-mouse, anti-rabbit, or anti-human IgG (Jackson ImmunoResearch Laboratories, West Grove, PA) at a dilution of 1:10,000 for 1h, washed, and visualized using SuperSignal West Pico substrate (Pierce, Rockford, IL). .. For stage specificity analysis of PfM6T expression, in vitro cultures were synchronized with 2 consecutive incubations in 5% sorbitol and cultured for indicated durations before harvesting for immunoblotting of matched samples.

Article Title: A novel panel of α-synuclein antibodies reveal distinctive staining profiles in synucleinopathies
Article Snippet: .. Following washing with TBS, blots were incubated with HRP conjugated goat anti-mouse or goat anti-rabbit secondary antibodies (Jackson Immuno Research Labs, West Grove, PA) diluted in 5% milk/TBS for 1 hour. .. Following washing with TBS, protein bands were visualized using Western Lightning-Plus ECL reagents (PerkinElmer, Waltham, MA) and images were captured using the GeneGnome XRQ system and GeneTools software (Syngene, Frederick, MD).

Blocking Assay:

Article Title: A Novel Helper Phage Enabling Construction of Genome-Scale ORF-Enriched Phage Display Libraries
Article Snippet: .. After blocking, the blots were probed with mouse anti-gIIIP MAb (30421) and a rabbit polyclonal antibody against whole M13 phage, followed by HRP-conjugated goat anti-mouse and goat anti-rabbit IgG (H+L) antibodies (Jackson Immuno-Research Laboratories, West Grove, PA, USA), respectively, and the bands were visualized using 3,3′-diaminobenzidine (DAB) as a substrate. .. Phagemid Display Vector and Gene Fragment Libraries The high copy number phagemid vector pVCEPI23964 (Fig. S1 in ) has a backbone derived from pUC119 and carries (under the control of lac PO between Hind III and Eco RI sites) a DNA cassette comprising a Xba I site, ribosome binding site (RBS), pectate lyase B signal sequence (PelBss ), 1.8 kb stuffer flanked by unique restriction sites followed by the codons for a trypsin cleavage site (KDIR) and full length gIIIP (2–405 codons), with appropriate spacers comprising glycine and serine residues.

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  • 85
    Jackson Immuno horseradish peroxide conjugated goat anti mouse antibody
    Disulfide-linked oligomer formation of BM2 TM domain cysteine substitution mutants in the absence of CuP treatment. Oocytes were injected with mRNA, and gel samples were prepared as described under “Experimental Procedures.” The samples were loaded on a 12% SDS-PAGE and transferred onto nitrocellulose membrane. The fusion BM2 proteins were probed with <t>anti-FLAG</t> antibody followed by <t>horseradish</t> <t>peroxidase-conjugated</t> <t>goat</t> <t>anti-mouse</t> IgG antibody. A , representative Western blot figure. Protein markers are labeled on the left side in kilodaltons. Bands that correspond to the molecular weight of dimers ( asterisks ) and trimers ( arrowhead ) are labeled to the left of the bands. A sample prepared from uninjected cells was used as a control ( leftmost and rightmost lanes ). B , a graph showing percentages of dimers and trimers of each mutant BM2. A percentage is expressed as a fraction of the species relative to total protein.
    Horseradish Peroxide Conjugated Goat Anti Mouse Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 85/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxide conjugated goat anti mouse antibody/product/Jackson Immuno
    Average 85 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
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    85/100 stars
      Buy from Supplier

    92
    Jackson Immuno hrp conjugated goat anti rabbit igg
    The eLtaS-specific monoclonal antibody MAE4 efficiently prevents S. aureus infection. ( a ) The binding affinity of MAE4 to eLtaS was determined by ELISA. eLtaS protein was coated onto 96-well plates in the presence of a serial dilution of MAE4. Bound <t>IgG</t> was detected using peroxidase <t>(HRP)-conjugated</t> goat anti-mouse IgG. ( b,c ) Assessment of the anti-infective effect of MAE4 in a murine model of acute peritoneal infection. MAE4 (100 μg/mouse) was injected into the peritoneal cavity 2 h prior to challenge with S. aureus 8325-4 (5 × 10 8 cfu/mouse) ( b ) or Δ ltaS (6 × 10 9 cfu/mouse) ( c ). The survival rate was measured at different time points post challenge. Data are presented as the percentage of mice surviving. Survival curves were determined using the Kaplan-Meier method and compared using the log-rank test (n = 8). ( d ) MAE4 protects mice from S. aureus infection in a pneumonia infection model. CD-1 mice were injected intratracheally with S. aureus 8325-4 cells (1 × 10 7 cfu/mouse). MAE4 IgG (100 μg/mouse) was injected intramuscularly into the left hind leg at 30 min, 24 h, and 48 h post infection. Lung sections were taken 72 h post challenge and stained with hematoxylin-eosin. ( e–g ) Determination of the effect of MAE4 in a sublethal murine model of intravenous challenge. MAE4 IgG (100 μg/mouse) was injected into the peritoneal cavity of BALB/c mice 2 h prior to intravenous challenge with S. aureus Newman (1 × 10 7 cfu/mouse). Kidneys were examined by histopathology for internal abscesses 5 days post infection. Staphylococcal abscess (black arrow) with a central concentration of staphylococci (blue arrow) is indicated ( e ). Bacterial colonies were counted at 5 days post infection ( f ). Data are representative of three independent experiments and shown as the mean ± SD. Significant differences between groups were evaluated using a two-tailed Student’s t test. For the lethal challenge model, S. aureus Newman cells (1 × 10 8 cfu/mouse) were administered and survival rates were monitored for 10 days ( g ). Survival curves were determined using the Kaplan-Meier method and compared using the log-rank test (n = 8) (NS, non-significant).
    Hrp Conjugated Goat Anti Rabbit Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 92/100, based on 111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated goat anti rabbit igg/product/Jackson Immuno
    Average 92 stars, based on 111 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated goat anti rabbit igg - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    85
    Jackson Immuno horseradish peroxidase hrp conjugated goat anti mouse igg igm
    a) ELISA titers of anti-Tn <t>IgG+IgM</t> antibody in mouse sera prior to immunization (day 0), after immunization with Tn-1-S-CPMV (day 35) in the absence and presence of 0.1 M GalNAc. b) ELISA titers of anti-Tn IgM and IgG antibody in mouse sera after immunization
    Horseradish Peroxidase Hrp Conjugated Goat Anti Mouse Igg Igm, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase hrp conjugated goat anti mouse igg igm/product/Jackson Immuno
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase hrp conjugated goat anti mouse igg igm - by Bioz Stars, 2020-09
    85/100 stars
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    Image Search Results


    Disulfide-linked oligomer formation of BM2 TM domain cysteine substitution mutants in the absence of CuP treatment. Oocytes were injected with mRNA, and gel samples were prepared as described under “Experimental Procedures.” The samples were loaded on a 12% SDS-PAGE and transferred onto nitrocellulose membrane. The fusion BM2 proteins were probed with anti-FLAG antibody followed by horseradish peroxidase-conjugated goat anti-mouse IgG antibody. A , representative Western blot figure. Protein markers are labeled on the left side in kilodaltons. Bands that correspond to the molecular weight of dimers ( asterisks ) and trimers ( arrowhead ) are labeled to the left of the bands. A sample prepared from uninjected cells was used as a control ( leftmost and rightmost lanes ). B , a graph showing percentages of dimers and trimers of each mutant BM2. A percentage is expressed as a fraction of the species relative to total protein.

    Journal: The Journal of Biological Chemistry

    Article Title: Identification of the Pore-lining Residues of the BM2 Ion Channel Protein of Influenza B Virus *Identification of the Pore-lining Residues of the BM2 Ion Channel Protein of Influenza B Virus * S⃞

    doi: 10.1074/jbc.M710302200

    Figure Lengend Snippet: Disulfide-linked oligomer formation of BM2 TM domain cysteine substitution mutants in the absence of CuP treatment. Oocytes were injected with mRNA, and gel samples were prepared as described under “Experimental Procedures.” The samples were loaded on a 12% SDS-PAGE and transferred onto nitrocellulose membrane. The fusion BM2 proteins were probed with anti-FLAG antibody followed by horseradish peroxidase-conjugated goat anti-mouse IgG antibody. A , representative Western blot figure. Protein markers are labeled on the left side in kilodaltons. Bands that correspond to the molecular weight of dimers ( asterisks ) and trimers ( arrowhead ) are labeled to the left of the bands. A sample prepared from uninjected cells was used as a control ( leftmost and rightmost lanes ). B , a graph showing percentages of dimers and trimers of each mutant BM2. A percentage is expressed as a fraction of the species relative to total protein.

    Article Snippet: The BM2 proteins were probed with ANTI-FLAG® M2 monoclonal antibody (F 3165; Sigma), followed by horseradish peroxide-conjugated goat anti-mouse antibody (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA).

    Techniques: Injection, SDS Page, Western Blot, Labeling, Molecular Weight, Mutagenesis

    JK-6L cells show enhanced protein accumulation in the presence of bortezomib and verapamil. (A) Western blot analysis of detergent-soluble and detergent-insoluble fractions of JK-6L cells treated with 10 nM bortezomib and/or 70 µ M verapamil for 16 hours. Immunoblot analysiswas done using an anti-ubiquitin antibody. To detect free ubiquitin, a denser gel was used. One representative immunoblot of two independent experiments is shown. (B) Western blot analysis of total cell lysates fromJK-6L cells treated with 10 nM bortezomib and/or 70 µMverapamil for 16 hours. Immunoblot analysis was done using HRP-conjugated anti-IgG antibody. One representative immunoblot of two independent experiments is shown. (C) IgG concentration in the supernatant of JK-6L cells treated with 10 nM bortezomib and/or 70 µ M verapamil for 16 hourswas determined by ELISA. The absorbance (OD)wasmeasured using a spectrophotometer. Mean values and SDwere calculated fromhexaplicates. Data represent one of two independently done experiments. Student's t -test for unpaired heteroscedastic samples was used for statistical analysis. # P

    Journal: Neoplasia (New York, N.Y.)

    Article Title: Calcium Channel Blocker Verapamil Enhances Endoplasmic Reticulum Stress and Cell Death Induced by Proteasome Inhibition in Myeloma Cells 1Calcium Channel Blocker Verapamil Enhances Endoplasmic Reticulum Stress and Cell Death Induced by Proteasome Inhibition in Myeloma Cells 1 2

    doi:

    Figure Lengend Snippet: JK-6L cells show enhanced protein accumulation in the presence of bortezomib and verapamil. (A) Western blot analysis of detergent-soluble and detergent-insoluble fractions of JK-6L cells treated with 10 nM bortezomib and/or 70 µ M verapamil for 16 hours. Immunoblot analysiswas done using an anti-ubiquitin antibody. To detect free ubiquitin, a denser gel was used. One representative immunoblot of two independent experiments is shown. (B) Western blot analysis of total cell lysates fromJK-6L cells treated with 10 nM bortezomib and/or 70 µMverapamil for 16 hours. Immunoblot analysis was done using HRP-conjugated anti-IgG antibody. One representative immunoblot of two independent experiments is shown. (C) IgG concentration in the supernatant of JK-6L cells treated with 10 nM bortezomib and/or 70 µ M verapamil for 16 hourswas determined by ELISA. The absorbance (OD)wasmeasured using a spectrophotometer. Mean values and SDwere calculated fromhexaplicates. Data represent one of two independently done experiments. Student's t -test for unpaired heteroscedastic samples was used for statistical analysis. # P

    Article Snippet: As secondary antibodies, we used HRP-conjugated goat anti-mouse immunoglobulinG(IgG), goat antirabbit IgG (Jackson Immunoresearch Laboratories, Inc, West Grove, PA), and donkey antigoat IgG (Santa Cruz Biotechnology).

    Techniques: Western Blot, Concentration Assay, Enzyme-linked Immunosorbent Assay, Spectrophotometry

    The eLtaS-specific monoclonal antibody MAE4 efficiently prevents S. aureus infection. ( a ) The binding affinity of MAE4 to eLtaS was determined by ELISA. eLtaS protein was coated onto 96-well plates in the presence of a serial dilution of MAE4. Bound IgG was detected using peroxidase (HRP)-conjugated goat anti-mouse IgG. ( b,c ) Assessment of the anti-infective effect of MAE4 in a murine model of acute peritoneal infection. MAE4 (100 μg/mouse) was injected into the peritoneal cavity 2 h prior to challenge with S. aureus 8325-4 (5 × 10 8 cfu/mouse) ( b ) or Δ ltaS (6 × 10 9 cfu/mouse) ( c ). The survival rate was measured at different time points post challenge. Data are presented as the percentage of mice surviving. Survival curves were determined using the Kaplan-Meier method and compared using the log-rank test (n = 8). ( d ) MAE4 protects mice from S. aureus infection in a pneumonia infection model. CD-1 mice were injected intratracheally with S. aureus 8325-4 cells (1 × 10 7 cfu/mouse). MAE4 IgG (100 μg/mouse) was injected intramuscularly into the left hind leg at 30 min, 24 h, and 48 h post infection. Lung sections were taken 72 h post challenge and stained with hematoxylin-eosin. ( e–g ) Determination of the effect of MAE4 in a sublethal murine model of intravenous challenge. MAE4 IgG (100 μg/mouse) was injected into the peritoneal cavity of BALB/c mice 2 h prior to intravenous challenge with S. aureus Newman (1 × 10 7 cfu/mouse). Kidneys were examined by histopathology for internal abscesses 5 days post infection. Staphylococcal abscess (black arrow) with a central concentration of staphylococci (blue arrow) is indicated ( e ). Bacterial colonies were counted at 5 days post infection ( f ). Data are representative of three independent experiments and shown as the mean ± SD. Significant differences between groups were evaluated using a two-tailed Student’s t test. For the lethal challenge model, S. aureus Newman cells (1 × 10 8 cfu/mouse) were administered and survival rates were monitored for 10 days ( g ). Survival curves were determined using the Kaplan-Meier method and compared using the log-rank test (n = 8) (NS, non-significant).

    Journal: Scientific Reports

    Article Title: MAE4, an eLtaS monoclonal antibody, blocks Staphylococcus aureus virulence

    doi: 10.1038/srep17215

    Figure Lengend Snippet: The eLtaS-specific monoclonal antibody MAE4 efficiently prevents S. aureus infection. ( a ) The binding affinity of MAE4 to eLtaS was determined by ELISA. eLtaS protein was coated onto 96-well plates in the presence of a serial dilution of MAE4. Bound IgG was detected using peroxidase (HRP)-conjugated goat anti-mouse IgG. ( b,c ) Assessment of the anti-infective effect of MAE4 in a murine model of acute peritoneal infection. MAE4 (100 μg/mouse) was injected into the peritoneal cavity 2 h prior to challenge with S. aureus 8325-4 (5 × 10 8 cfu/mouse) ( b ) or Δ ltaS (6 × 10 9 cfu/mouse) ( c ). The survival rate was measured at different time points post challenge. Data are presented as the percentage of mice surviving. Survival curves were determined using the Kaplan-Meier method and compared using the log-rank test (n = 8). ( d ) MAE4 protects mice from S. aureus infection in a pneumonia infection model. CD-1 mice were injected intratracheally with S. aureus 8325-4 cells (1 × 10 7 cfu/mouse). MAE4 IgG (100 μg/mouse) was injected intramuscularly into the left hind leg at 30 min, 24 h, and 48 h post infection. Lung sections were taken 72 h post challenge and stained with hematoxylin-eosin. ( e–g ) Determination of the effect of MAE4 in a sublethal murine model of intravenous challenge. MAE4 IgG (100 μg/mouse) was injected into the peritoneal cavity of BALB/c mice 2 h prior to intravenous challenge with S. aureus Newman (1 × 10 7 cfu/mouse). Kidneys were examined by histopathology for internal abscesses 5 days post infection. Staphylococcal abscess (black arrow) with a central concentration of staphylococci (blue arrow) is indicated ( e ). Bacterial colonies were counted at 5 days post infection ( f ). Data are representative of three independent experiments and shown as the mean ± SD. Significant differences between groups were evaluated using a two-tailed Student’s t test. For the lethal challenge model, S. aureus Newman cells (1 × 10 8 cfu/mouse) were administered and survival rates were monitored for 10 days ( g ). Survival curves were determined using the Kaplan-Meier method and compared using the log-rank test (n = 8) (NS, non-significant).

    Article Snippet: Bound C3b proteins were detected using anti-C3 (Santa Cruz Biotechnology) followed by HRP-conjugated goat anti-rabbit IgG (1:20,000, Jackson ImmunoResearch Laboratories).

    Techniques: Infection, Binding Assay, Enzyme-linked Immunosorbent Assay, Serial Dilution, Injection, Mouse Assay, Staining, Histopathology, Concentration Assay, Two Tailed Test

    a) ELISA titers of anti-Tn IgG+IgM antibody in mouse sera prior to immunization (day 0), after immunization with Tn-1-S-CPMV (day 35) in the absence and presence of 0.1 M GalNAc. b) ELISA titers of anti-Tn IgM and IgG antibody in mouse sera after immunization

    Journal: Chemistry (Weinheim an der Bergstrasse, Germany)

    Article Title: Cowpea Mosaic Virus Capsid, a Promising Carrier for the Development of Carbohydrate Based Anti-tumor Vaccines

    doi: 10.1002/chem.200

    Figure Lengend Snippet: a) ELISA titers of anti-Tn IgG+IgM antibody in mouse sera prior to immunization (day 0), after immunization with Tn-1-S-CPMV (day 35) in the absence and presence of 0.1 M GalNAc. b) ELISA titers of anti-Tn IgM and IgG antibody in mouse sera after immunization

    Article Snippet: A 1:2000 diluted horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG+IgM, IgG or IgM antibody (Jackson ImmunoResearch Laboratory IgG+IgM catalog #115-035-068, IgM #115-035-075, IgG #115-035-071) in 0.1% BSA/PBS was added to each well respectively.

    Techniques: Enzyme-linked Immunosorbent Assay