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GE Healthcare hrp conjugated goat anti mouse igg
Establishment of MAbs against M161Ag. (A) Western blot of M. fermentans PG18 with MAbs against M161Ag. Ten microliters of mycoplasma cell lysate (1.0 mg/ml) was applied to each lane. SDS-PAGE was performed under reducing conditions. Lane 1, Coomassie blue staining of M. fermentans PG18 proteins. Samples were transblotted onto nitrocellulose sheets and detected with the indicated MAbs (lanes 2 to 5). Mouse <t>IgG</t> was used as a control (lane 6). Molecular mass markers are shown to the left. (B) MAbs recognized the 43-kDa protein in P39(+) cells but not P39(−) cells. P39(+) and -(−) cell lysates were subjected to SDS-PAGE (10% gel) under reducing or nonreducing conditions and transferred onto nitrocellulose sheets. Each sheet was blotted with M161, MK53, MK5, or MK36 and then with <t>HRP-conjugated</t> goat anti-mouse IgG. NR, nonreducing conditions; R, reducing conditions. The positions of molecular mass markers are shown on the right. (C) Flow cytometric analysis of M161Ag on the P39 sublines. Cell surface M161Ag was assessed using flow cytometry with each MAb followed by a FITC-labeled anti-mouse IgG using P39(+) and -(−) cells.
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1) Product Images from "Complement Activation in Mycoplasma fermentans-Induced Mycoplasma Clearance from Infected Cells: Probing of the Organism with Monoclonal Antibodies against M161Ag"

Article Title: Complement Activation in Mycoplasma fermentans-Induced Mycoplasma Clearance from Infected Cells: Probing of the Organism with Monoclonal Antibodies against M161Ag

Journal: Infection and Immunity

doi:

Establishment of MAbs against M161Ag. (A) Western blot of M. fermentans PG18 with MAbs against M161Ag. Ten microliters of mycoplasma cell lysate (1.0 mg/ml) was applied to each lane. SDS-PAGE was performed under reducing conditions. Lane 1, Coomassie blue staining of M. fermentans PG18 proteins. Samples were transblotted onto nitrocellulose sheets and detected with the indicated MAbs (lanes 2 to 5). Mouse IgG was used as a control (lane 6). Molecular mass markers are shown to the left. (B) MAbs recognized the 43-kDa protein in P39(+) cells but not P39(−) cells. P39(+) and -(−) cell lysates were subjected to SDS-PAGE (10% gel) under reducing or nonreducing conditions and transferred onto nitrocellulose sheets. Each sheet was blotted with M161, MK53, MK5, or MK36 and then with HRP-conjugated goat anti-mouse IgG. NR, nonreducing conditions; R, reducing conditions. The positions of molecular mass markers are shown on the right. (C) Flow cytometric analysis of M161Ag on the P39 sublines. Cell surface M161Ag was assessed using flow cytometry with each MAb followed by a FITC-labeled anti-mouse IgG using P39(+) and -(−) cells.
Figure Legend Snippet: Establishment of MAbs against M161Ag. (A) Western blot of M. fermentans PG18 with MAbs against M161Ag. Ten microliters of mycoplasma cell lysate (1.0 mg/ml) was applied to each lane. SDS-PAGE was performed under reducing conditions. Lane 1, Coomassie blue staining of M. fermentans PG18 proteins. Samples were transblotted onto nitrocellulose sheets and detected with the indicated MAbs (lanes 2 to 5). Mouse IgG was used as a control (lane 6). Molecular mass markers are shown to the left. (B) MAbs recognized the 43-kDa protein in P39(+) cells but not P39(−) cells. P39(+) and -(−) cell lysates were subjected to SDS-PAGE (10% gel) under reducing or nonreducing conditions and transferred onto nitrocellulose sheets. Each sheet was blotted with M161, MK53, MK5, or MK36 and then with HRP-conjugated goat anti-mouse IgG. NR, nonreducing conditions; R, reducing conditions. The positions of molecular mass markers are shown on the right. (C) Flow cytometric analysis of M161Ag on the P39 sublines. Cell surface M161Ag was assessed using flow cytometry with each MAb followed by a FITC-labeled anti-mouse IgG using P39(+) and -(−) cells.

Techniques Used: Western Blot, SDS Page, Staining, Flow Cytometry, Cytometry, Labeling

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Centrifugation:

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Binding Assay:

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Positive Control:

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Blocking Assay:

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Enzyme-linked Immunosorbent Assay:

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Incubation:

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Stripping Membranes:

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Expressing:

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Cell Fractionation:

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Western Blot:

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Transfection:

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Chromatography:

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Concentration Assay:

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Infection:

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Imaging:

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Sonication:

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Affinity Purification:

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Recombinant:

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Nucleic Acid Electrophoresis:

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Article Title: Complement Activation in Mycoplasma fermentans-Induced Mycoplasma Clearance from Infected Cells: Probing of the Organism with Monoclonal Antibodies against M161Ag
Article Snippet: Aliquots of 50 μl of the supernatant were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (10% gel) under nonreducing or reducing conditions. .. The sheets were then blocked with 10% skim milk for 1 h at 37°C and then overnight at 4°C and sequentially incubated with MAb and HRP-conjugated goat anti-mouse IgG, followed by staining with an ECL kit (Amersham Pharmacia Biotech).

Mutagenesis:

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Immunodetection:

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Mouse Assay:

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Indirect ELISA:

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Staining:

Article Title: Complement Activation in Mycoplasma fermentans-Induced Mycoplasma Clearance from Infected Cells: Probing of the Organism with Monoclonal Antibodies against M161Ag
Article Snippet: .. The sheets were then blocked with 10% skim milk for 1 h at 37°C and then overnight at 4°C and sequentially incubated with MAb and HRP-conjugated goat anti-mouse IgG, followed by staining with an ECL kit (Amersham Pharmacia Biotech). ..

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SDS Page:

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Article Title: Complement Activation in Mycoplasma fermentans-Induced Mycoplasma Clearance from Infected Cells: Probing of the Organism with Monoclonal Antibodies against M161Ag
Article Snippet: Aliquots of 50 μl of the supernatant were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (10% gel) under nonreducing or reducing conditions. .. The sheets were then blocked with 10% skim milk for 1 h at 37°C and then overnight at 4°C and sequentially incubated with MAb and HRP-conjugated goat anti-mouse IgG, followed by staining with an ECL kit (Amersham Pharmacia Biotech).

Electrophoresis:

Article Title: Complement Activation in Mycoplasma fermentans-Induced Mycoplasma Clearance from Infected Cells: Probing of the Organism with Monoclonal Antibodies against M161Ag
Article Snippet: After electrophoresis, the resolved proteins were transferred onto nitrocellulose sheets. .. The sheets were then blocked with 10% skim milk for 1 h at 37°C and then overnight at 4°C and sequentially incubated with MAb and HRP-conjugated goat anti-mouse IgG, followed by staining with an ECL kit (Amersham Pharmacia Biotech).

Positron Emission Tomography:

Article Title: The pp24 phosphoprotein of Mason-Pfizer monkey virus contributes to viral genome packaging
Article Snippet: HRP-conjugated goat anti-rabbit IgG and HRP-conjugated goat anti-mouse IgG were purchased from Amersham Pharmacia Biotech (Little Chalfont Buckinghamshire, England). .. M-PMV Gag proteins containing a carboxy-terminal (His)6 tag were expressed in E. coli BL21 (DE3) cells from the expression plamsid pET.M100A for 4 hours in the presence of 0.1 mM IPTG.

Sample Prep:

Article Title: An HPV 16 L1-based chimeric human papilloma virus-like particles containing a string of epitopes produced in plants is able to elicit humoral and cytotoxic T-cell activity in mice
Article Snippet: Paragraph title: Sample preparation and western blot analysis ... Mouse monoclonal anti-L1HPV 16 (Chemicon) at 1:500 and HRP conjugated goat anti mouse IgG (Amersham Pharmacia Biotech) at 1:10,000 were used for L1 and LI-E6/E7 HPV 16 detection, which was carried out by chemiluminescence (ECL Reagent, Amersham Pharmacia Biotech).

Spectrophotometry:

Article Title: Nuclear factor-?B contributes to interleukin-4- and interferon-dependent polymeric immunoglobulin receptor expression in human intestinal epithelial cells
Article Snippet: Cultures were washed twice with DPBS/Ca2+ /Mg2+ , once with Blocking Buffer, and then incubated for 1 hr at room temperature with HRP-conjugated goat anti-mouse IgG (Amersham Life Sciences; 1: 2000 in blocking buffer). .. The reaction was stopped by addition of 100 µl 0.5 m H2 SO4 and the absorbance at 450 nm was determined using a Bio-Rad microplate spectrophotometer.

Activation Assay:

Article Title: Nuclear factor-?B contributes to interleukin-4- and interferon-dependent polymeric immunoglobulin receptor expression in human intestinal epithelial cells
Article Snippet: Cultures were washed twice with DPBS/Ca2+ /Mg2+ , once with Blocking Buffer, and then incubated for 1 hr at room temperature with HRP-conjugated goat anti-mouse IgG (Amersham Life Sciences; 1: 2000 in blocking buffer). .. To verify that over-expressing IκBα-serine mutant inhibited NF-κB activation, cells were seeded at 2 × 105 cells/well in 24-well plates and allowed to attach overnight.

Lysis:

Article Title: Complement Activation in Mycoplasma fermentans-Induced Mycoplasma Clearance from Infected Cells: Probing of the Organism with Monoclonal Antibodies against M161Ag
Article Snippet: Cells (107 ) were lysed in 200 μl of lysis buffer (1% NP-40, 10 mM EDTA, 25 mM iodoacetoamide, 2 mM phenylmethylsulfonyl fluoride, DPBS) for 20 min at room temperature. .. The sheets were then blocked with 10% skim milk for 1 h at 37°C and then overnight at 4°C and sequentially incubated with MAb and HRP-conjugated goat anti-mouse IgG, followed by staining with an ECL kit (Amersham Pharmacia Biotech).

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    GE Healthcare horse radish peroxidase hrp conjugated goat anti mouse igg antibody
    Interaction between ESA-2 and influenza virus glycoprotein HA. ( A ) Binding interaction between ESA-2 and HA was analyzed by an ELISA assay. Various dilutions of influenza vaccine preparation which contain HA mixture of A/California/7/09 (H1N1), A/Victoria/210/09 (H3N2), and B/Brisbane/60/08 was added to ESA-2 immobilized plate. Interactions were detected by incubating with mouse anti-HA monoclonal antibody followed by incubation with <t>HRP-conjugated</t> goat anti-mouse <t>IgG</t> antibody. The colorimetric substrate (TMB) was added to each well and the absorbance at 450 nm was measured. Yeast mannan (YM) was used as a reference; ( B ) Inhibition assay was performed using YM with aforementioned methods, except that the ESA-2 coated plate was pre-incubated with various concentrations of YM for 1 h at room temperature before adding vaccine preparation containing HA mixture.
    Horse Radish Peroxidase Hrp Conjugated Goat Anti Mouse Igg Antibody, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 87/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horse radish peroxidase hrp conjugated goat anti mouse igg antibody/product/GE Healthcare
    Average 87 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    horse radish peroxidase hrp conjugated goat anti mouse igg antibody - by Bioz Stars, 2020-04
    87/100 stars
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    91
    GE Healthcare horse radish peroxidase conjugated goat anti rat igg antibodies
    <t>IgG</t> responses of subjects vaccinated with BM32 or placebo and of hepatitis B virus-infected individuals specific for preS peptides. Shown are optical density values (y-axes: OD values) of IgG levels specific for preS-derived peptides (P1–P8) of subjects immunized with placebo (n = 8), 20 μg (n = 10) or 40 μg of BM32 (n = 12) at visit 15 as well as of HBV-infected individuals (n = 19) (x-axes). Medians are indicated by horizontal lines. P1 (aa 2–31); P2 (aa 22–51); P3 (aa 42–71); P4 (aa 62–91); P5 (aa 82–111); P6 (aa 102–131); P7 (aa 122–151); P8 (aa 142–174).
    Horse Radish Peroxidase Conjugated Goat Anti Rat Igg Antibodies, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horse radish peroxidase conjugated goat anti rat igg antibodies/product/GE Healthcare
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    horse radish peroxidase conjugated goat anti rat igg antibodies - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

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    Interaction between ESA-2 and influenza virus glycoprotein HA. ( A ) Binding interaction between ESA-2 and HA was analyzed by an ELISA assay. Various dilutions of influenza vaccine preparation which contain HA mixture of A/California/7/09 (H1N1), A/Victoria/210/09 (H3N2), and B/Brisbane/60/08 was added to ESA-2 immobilized plate. Interactions were detected by incubating with mouse anti-HA monoclonal antibody followed by incubation with HRP-conjugated goat anti-mouse IgG antibody. The colorimetric substrate (TMB) was added to each well and the absorbance at 450 nm was measured. Yeast mannan (YM) was used as a reference; ( B ) Inhibition assay was performed using YM with aforementioned methods, except that the ESA-2 coated plate was pre-incubated with various concentrations of YM for 1 h at room temperature before adding vaccine preparation containing HA mixture.

    Journal: Marine Drugs

    Article Title: Entry Inhibition of Influenza Viruses with High Mannose Binding Lectin ESA-2 from the Red Alga Eucheuma serra through the Recognition of Viral Hemagglutinin

    doi: 10.3390/md13063454

    Figure Lengend Snippet: Interaction between ESA-2 and influenza virus glycoprotein HA. ( A ) Binding interaction between ESA-2 and HA was analyzed by an ELISA assay. Various dilutions of influenza vaccine preparation which contain HA mixture of A/California/7/09 (H1N1), A/Victoria/210/09 (H3N2), and B/Brisbane/60/08 was added to ESA-2 immobilized plate. Interactions were detected by incubating with mouse anti-HA monoclonal antibody followed by incubation with HRP-conjugated goat anti-mouse IgG antibody. The colorimetric substrate (TMB) was added to each well and the absorbance at 450 nm was measured. Yeast mannan (YM) was used as a reference; ( B ) Inhibition assay was performed using YM with aforementioned methods, except that the ESA-2 coated plate was pre-incubated with various concentrations of YM for 1 h at room temperature before adding vaccine preparation containing HA mixture.

    Article Snippet: The wells were incubated with mouse anti-HA monoclonal antibody (HyTest) followed by incubation with horse-radish peroxidase (HRP)-conjugated goat anti-mouse IgG antibody (GE Healthcare, Buckinghamshire, UK).

    Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Incubation, Inhibition

    IgG responses of subjects vaccinated with BM32 or placebo and of hepatitis B virus-infected individuals specific for preS peptides. Shown are optical density values (y-axes: OD values) of IgG levels specific for preS-derived peptides (P1–P8) of subjects immunized with placebo (n = 8), 20 μg (n = 10) or 40 μg of BM32 (n = 12) at visit 15 as well as of HBV-infected individuals (n = 19) (x-axes). Medians are indicated by horizontal lines. P1 (aa 2–31); P2 (aa 22–51); P3 (aa 42–71); P4 (aa 62–91); P5 (aa 82–111); P6 (aa 102–131); P7 (aa 122–151); P8 (aa 142–174).

    Journal: EBioMedicine

    Article Title: Immunotherapy With the PreS-based Grass Pollen Allergy Vaccine BM32 Induces Antibody Responses Protecting Against Hepatitis B Infection

    doi: 10.1016/j.ebiom.2016.07.023

    Figure Lengend Snippet: IgG responses of subjects vaccinated with BM32 or placebo and of hepatitis B virus-infected individuals specific for preS peptides. Shown are optical density values (y-axes: OD values) of IgG levels specific for preS-derived peptides (P1–P8) of subjects immunized with placebo (n = 8), 20 μg (n = 10) or 40 μg of BM32 (n = 12) at visit 15 as well as of HBV-infected individuals (n = 19) (x-axes). Medians are indicated by horizontal lines. P1 (aa 2–31); P2 (aa 22–51); P3 (aa 42–71); P4 (aa 62–91); P5 (aa 82–111); P6 (aa 102–131); P7 (aa 122–151); P8 (aa 142–174).

    Article Snippet: Bound mouse IgG1 was detected by monoclonal rat anti-mouse IgG1 (BD Biosciences Cat# 553440 RRID: AB_394860 ) diluted 1:1000, followed by horse radish peroxidase-conjugated goat anti-rat IgG antibodies (GE Healthcare Cat# NA935 RRID: AB_772207 ), diluted 1:2,500.

    Techniques: Infection, Derivative Assay

    Overview of the treatment period of the BM32 trial. (a) Subjects received seven injections of placebo or BM32 over two years as depicted in the time line. Visits during which blood samples were obtained are indicated. (b) IgG responses of subjects vaccinated with BM32 or placebo towards preS and synthetic preS-derived overlapping peptides. Shown are optical density values (y-axes: OD values, means of triplicate determination) corresponding to IgG levels towards preS and peptides P1–P8 measured in subjects with (red symbols) or without (black symbols) prior HBV vaccination who had been immunized with BM32 or placebo before (V5) and at different time points after immunization (V8 and V15) (x-axes). Medians (horizontal bars) and significant differences are indicated: * P

    Journal: EBioMedicine

    Article Title: Immunotherapy With the PreS-based Grass Pollen Allergy Vaccine BM32 Induces Antibody Responses Protecting Against Hepatitis B Infection

    doi: 10.1016/j.ebiom.2016.07.023

    Figure Lengend Snippet: Overview of the treatment period of the BM32 trial. (a) Subjects received seven injections of placebo or BM32 over two years as depicted in the time line. Visits during which blood samples were obtained are indicated. (b) IgG responses of subjects vaccinated with BM32 or placebo towards preS and synthetic preS-derived overlapping peptides. Shown are optical density values (y-axes: OD values, means of triplicate determination) corresponding to IgG levels towards preS and peptides P1–P8 measured in subjects with (red symbols) or without (black symbols) prior HBV vaccination who had been immunized with BM32 or placebo before (V5) and at different time points after immunization (V8 and V15) (x-axes). Medians (horizontal bars) and significant differences are indicated: * P

    Article Snippet: Bound mouse IgG1 was detected by monoclonal rat anti-mouse IgG1 (BD Biosciences Cat# 553440 RRID: AB_394860 ) diluted 1:1000, followed by horse radish peroxidase-conjugated goat anti-rat IgG antibodies (GE Healthcare Cat# NA935 RRID: AB_772207 ), diluted 1:2,500.

    Techniques: Derivative Assay

    IgG responses towards preS and synthetic preS-derived overlapping peptides. Optical density values (y-axes: OD values at 405 nm) corresponding to IgG levels of rabbits immunized with (a) preS (n = 1), (b) 20 μg of BM32 (n = 2), (c) 40 μg of BM32 (n = 2) or to IgG 1 levels of groups of mice (n = 6) immunized with (d) 10 μg, (e) 20 μg or (f) 30 μg of BM32 prior to (grey bars) and after (black bars) immunization, towards preS and synthetic preS overlapping peptides P1–P8 (x-axes). Results represent medians ± interquartile ranges from triplicate determinations.

    Journal: EBioMedicine

    Article Title: Immunotherapy With the PreS-based Grass Pollen Allergy Vaccine BM32 Induces Antibody Responses Protecting Against Hepatitis B Infection

    doi: 10.1016/j.ebiom.2016.07.023

    Figure Lengend Snippet: IgG responses towards preS and synthetic preS-derived overlapping peptides. Optical density values (y-axes: OD values at 405 nm) corresponding to IgG levels of rabbits immunized with (a) preS (n = 1), (b) 20 μg of BM32 (n = 2), (c) 40 μg of BM32 (n = 2) or to IgG 1 levels of groups of mice (n = 6) immunized with (d) 10 μg, (e) 20 μg or (f) 30 μg of BM32 prior to (grey bars) and after (black bars) immunization, towards preS and synthetic preS overlapping peptides P1–P8 (x-axes). Results represent medians ± interquartile ranges from triplicate determinations.

    Article Snippet: Bound mouse IgG1 was detected by monoclonal rat anti-mouse IgG1 (BD Biosciences Cat# 553440 RRID: AB_394860 ) diluted 1:1000, followed by horse radish peroxidase-conjugated goat anti-rat IgG antibodies (GE Healthcare Cat# NA935 RRID: AB_772207 ), diluted 1:2,500.

    Techniques: Derivative Assay, Mouse Assay

    PreS-specific antibody responses of subjects vaccinated with BM32 or placebo and of HBV-infected individuals. Shown are optical density values (y-axes: OD values) of IgA, IgE, IgM, IgG and IgG subclass (IgG 1 –IgG 4 ) levels specific for preS of subjects immunized with placebo (n = 8), 20 μg (n = 10) or 40 μg of BM32 (n = 12) at visit 15 as well as of HBV-infected individuals (n = 19) (x-axes). Medians are indicated by horizontal lines, significant differences are indicated: *** P

    Journal: EBioMedicine

    Article Title: Immunotherapy With the PreS-based Grass Pollen Allergy Vaccine BM32 Induces Antibody Responses Protecting Against Hepatitis B Infection

    doi: 10.1016/j.ebiom.2016.07.023

    Figure Lengend Snippet: PreS-specific antibody responses of subjects vaccinated with BM32 or placebo and of HBV-infected individuals. Shown are optical density values (y-axes: OD values) of IgA, IgE, IgM, IgG and IgG subclass (IgG 1 –IgG 4 ) levels specific for preS of subjects immunized with placebo (n = 8), 20 μg (n = 10) or 40 μg of BM32 (n = 12) at visit 15 as well as of HBV-infected individuals (n = 19) (x-axes). Medians are indicated by horizontal lines, significant differences are indicated: *** P

    Article Snippet: Bound mouse IgG1 was detected by monoclonal rat anti-mouse IgG1 (BD Biosciences Cat# 553440 RRID: AB_394860 ) diluted 1:1000, followed by horse radish peroxidase-conjugated goat anti-rat IgG antibodies (GE Healthcare Cat# NA935 RRID: AB_772207 ), diluted 1:2,500.

    Techniques: Infection