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Bio-Rad hrp conjugated goat anti human igg
Hrp Conjugated Goat Anti Human Igg, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hrp conjugated goat anti human igg/product/Bio-Rad
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
hrp conjugated goat anti human igg - by Bioz Stars, 2020-04
93/100 stars

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Nucleic Acid Electrophoresis:

Article Title: Leucine Aminopeptidase Is an Immunodominant Antigen of Fasciola hepatica Excretory and Secretory Products in Human Infections ▿
Article Snippet: Proteins from SDS-polyacrylamide gel electrophoresis (PAGE) were stained with Coomassie blue or electroblotted onto nitrocellulose membranes. .. Bound antibodies were detected by incubating blots for 1 h at room temperature with HRP-conjugated goat anti-human IgG (Bio-Rad) in TBST-1% BSA.

Blocking Assay:

Article Title: Leucine Aminopeptidase Is an Immunodominant Antigen of Fasciola hepatica Excretory and Secretory Products in Human Infections ▿
Article Snippet: Bound antibodies were detected by incubating blots for 1 h at room temperature with HRP-conjugated goat anti-human IgG (Bio-Rad) in TBST-1% BSA. .. Filters were stripped at 50°C for 30 min in a solution containing 100 mM of 2-mercaptoethanol and 2% SDS in 62.5 mM Tris-HCl, pH 6.7, followed by extensive washing and another blocking step prior to reprobing, either with human antisera (1:200 in TBS plus 3% BSA) or with rabbit serum against rLAPFh (1:1,000 in TBS plus 1% BSA), followed by extensive washing and incubation with secondary antibodies coupled to HRP (1:10,000 in TBS plus 1% BSA).

Incubation:

Article Title: Leucine Aminopeptidase Is an Immunodominant Antigen of Fasciola hepatica Excretory and Secretory Products in Human Infections ▿
Article Snippet: After being extensively washed with TBS containing 0.05% Tween-20 (TBST), blots were incubated overnight at 4°C with pooled human sera (1:200 in TBS plus 3% BSA). .. Bound antibodies were detected by incubating blots for 1 h at room temperature with HRP-conjugated goat anti-human IgG (Bio-Rad) in TBST-1% BSA.

Article Title: Decreased Frequencies of Circulating Follicular Helper T Cell Counterparts and Plasmablasts in Ankylosing Spondylitis Patients Naïve for TNF Blockers
Article Snippet: .. Standard curves of human IgG, IgA or IgM (Sigma-Aldrich) together with culture supernatants diluted in 2% BSA/PBS were incubated for 3 hours at room temperature, washed and developed with HRP-conjugated goat anti-human IgG, IgA or IgM (ABD serotec) followed by TMB substrate solution (BD-Pharmingen). ..

Stripping Membranes:

Article Title: Leucine Aminopeptidase Is an Immunodominant Antigen of Fasciola hepatica Excretory and Secretory Products in Human Infections ▿
Article Snippet: Paragraph title: SDS-PAGE analysis, Western blot analysis, and stripping. ... Bound antibodies were detected by incubating blots for 1 h at room temperature with HRP-conjugated goat anti-human IgG (Bio-Rad) in TBST-1% BSA.

Polyacrylamide Gel Electrophoresis:

Article Title: Leucine Aminopeptidase Is an Immunodominant Antigen of Fasciola hepatica Excretory and Secretory Products in Human Infections ▿
Article Snippet: Proteins from SDS-polyacrylamide gel electrophoresis (PAGE) were stained with Coomassie blue or electroblotted onto nitrocellulose membranes. .. Bound antibodies were detected by incubating blots for 1 h at room temperature with HRP-conjugated goat anti-human IgG (Bio-Rad) in TBST-1% BSA.

Western Blot:

Article Title: Leucine Aminopeptidase Is an Immunodominant Antigen of Fasciola hepatica Excretory and Secretory Products in Human Infections ▿
Article Snippet: Paragraph title: SDS-PAGE analysis, Western blot analysis, and stripping. ... Bound antibodies were detected by incubating blots for 1 h at room temperature with HRP-conjugated goat anti-human IgG (Bio-Rad) in TBST-1% BSA.

Staining:

Article Title: Leucine Aminopeptidase Is an Immunodominant Antigen of Fasciola hepatica Excretory and Secretory Products in Human Infections ▿
Article Snippet: Filters were stained with 0.1% Ponceau S (Sigma) for 10 min and blocked for 2 h in 10 mM Tris-HCl, pH 7.4, 150 mM NaCl (TBS) containing 3% bovine serum albumin (BSA) (Roche). .. Bound antibodies were detected by incubating blots for 1 h at room temperature with HRP-conjugated goat anti-human IgG (Bio-Rad) in TBST-1% BSA.

Electrofocusing:

Article Title: Leucine Aminopeptidase Is an Immunodominant Antigen of Fasciola hepatica Excretory and Secretory Products in Human Infections ▿
Article Snippet: After electrofocusing, the strips were reduced (2% DTT) and then alkylated (2.5% iodoacetamide) in equilibration buffer containing 6 M urea, 0.375 M Tris, pH 8.8, 2% sodium dodecyl sulfate (SDS), and 20% glycerol, and the second dimension was performed using 10% polyacrylamide gels. .. Bound antibodies were detected by incubating blots for 1 h at room temperature with HRP-conjugated goat anti-human IgG (Bio-Rad) in TBST-1% BSA.

SDS Page:

Article Title: Leucine Aminopeptidase Is an Immunodominant Antigen of Fasciola hepatica Excretory and Secretory Products in Human Infections ▿
Article Snippet: Paragraph title: SDS-PAGE analysis, Western blot analysis, and stripping. ... Bound antibodies were detected by incubating blots for 1 h at room temperature with HRP-conjugated goat anti-human IgG (Bio-Rad) in TBST-1% BSA.

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    Bio-Rad horseradish peroxidase hrp conjugated goat anti rabbit antibody
    Representative example of <t>OFA</t> immune response in vivo . The lanes contain protein extracts (40 μg per lane) from OFA-expressing 4T1 cells, incubated with sera from mice orally immunized with wild-type (wt) L. plantarum or L. plantarum harboring pLp_0373sOFAcwa2 (cwa2), or from mice subcutaneously immunized with His-tagged OFA in Freund's complete adjuvant (OFA-His). Anti-OFA monoclonal mouse IgG antibody (mAb) was used as a positive control. Antibody binding was visualized using <t>HRP</t> conjugated to anti-mouse IgG and the ECL system (Amersham Life Science, Buckinghamshire, United Kingdom). The arrow indicates OFA.
    Horseradish Peroxidase Hrp Conjugated Goat Anti Rabbit Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase hrp conjugated goat anti rabbit antibody/product/Bio-Rad
    Average 99 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase hrp conjugated goat anti rabbit antibody - by Bioz Stars, 2020-04
    99/100 stars
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    Representative example of OFA immune response in vivo . The lanes contain protein extracts (40 μg per lane) from OFA-expressing 4T1 cells, incubated with sera from mice orally immunized with wild-type (wt) L. plantarum or L. plantarum harboring pLp_0373sOFAcwa2 (cwa2), or from mice subcutaneously immunized with His-tagged OFA in Freund's complete adjuvant (OFA-His). Anti-OFA monoclonal mouse IgG antibody (mAb) was used as a positive control. Antibody binding was visualized using HRP conjugated to anti-mouse IgG and the ECL system (Amersham Life Science, Buckinghamshire, United Kingdom). The arrow indicates OFA.

    Journal: Applied and Environmental Microbiology

    Article Title: Cell Wall Anchoring of the 37-Kilodalton Oncofetal Antigen by Lactobacillus plantarum for Mucosal Cancer Vaccine Delivery ▿

    doi: 10.1128/AEM.01031-10

    Figure Lengend Snippet: Representative example of OFA immune response in vivo . The lanes contain protein extracts (40 μg per lane) from OFA-expressing 4T1 cells, incubated with sera from mice orally immunized with wild-type (wt) L. plantarum or L. plantarum harboring pLp_0373sOFAcwa2 (cwa2), or from mice subcutaneously immunized with His-tagged OFA in Freund's complete adjuvant (OFA-His). Anti-OFA monoclonal mouse IgG antibody (mAb) was used as a positive control. Antibody binding was visualized using HRP conjugated to anti-mouse IgG and the ECL system (Amersham Life Science, Buckinghamshire, United Kingdom). The arrow indicates OFA.

    Article Snippet: System (Millipore, Billerica, MA), using anti-laminin R polyclonal antibodies (Santa Cruz Biotechnology, Inc., CA) or OFA monoclonal antibodies combined with horseradish peroxidase (HRP)-conjugated goat anti-rabbit antibody (Bio-Rad) (Fig. A and B) or rabbit anti-mouse antibody (Dako, Denmark) (Fig. ), respectively.

    Techniques: In Vivo, Expressing, Incubation, Mouse Assay, Plasmid Purification, Positive Control, Binding Assay