hrp conjugate  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Thermo Fisher hrp conjugate
    Hrp Conjugate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugate/product/Thermo Fisher
    Average 99 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    hrp conjugate - by Bioz Stars, 2020-02
    99/100 stars

    Images

    Related Articles

    Amplification:

    Article Title: Production and evaluation of parathyroid hormone receptor1 ligands with intrinsic or assembled peroxidase domains
    Article Snippet: Following stimulation with the appropriate CM as described above, cells were rinsed 3 times with HBSS before being incubated with 100 mM H2 O2 and biotinyl-tyramide (from the Tyramide Signal Amplification kit, ThermoFisher, used as directed) or 500 µM biotin-phenol in the TSA amplification buffer from the same kit for 15 minutes at room temperature. .. Then, the cells were incubated 30 minutes with a HBSS solution containing 1% BSA to block the non-specific binding sites before being incubated with a horse radish peroxidase conjugate of streptavidin (SA-HRP; Invitrogen) for 1 hour.

    Article Title: Minimal 2'-O-methyl phosphorothioate linkage modification pattern of synthetic guide RNAs for increased stability and efficient CRISPR-Cas9 gene editing avoiding cellular toxicity
    Article Snippet: Paragraph title: Supporting information Sequences of synthetic sgRNA, crRNA and tracrRNA, including modification locations. PCR primers for amplification of gRNA target sites from genomic DNA for DNA mismatch detection assay. Unmodified synthetic sgRNA require a sequential electroporation method with Cas9 mRNA for efficient gene editing. Cas9 protein levels are detectable 4 hours after electroporation and are similar regardless of electroporation delivery method. MS modified guide RNAs perform similarly to unmodified in a stably expressing Cas9 cell line with some toxicity observed at higher concentrations. ... Membranes were washed and secondary antibody [goat anti-mouse IgG (H+L) Secondary Antibody, HRP conjugate (Thermo Scientific, Cat #32430))] was diluted 1:20,000 in SuperBlock (PBS formulation) with 0.5% Tween20 and incubated with membranes for 2 hours at room temperature.

    Mass Spectrometry:

    Article Title: Minimal 2'-O-methyl phosphorothioate linkage modification pattern of synthetic guide RNAs for increased stability and efficient CRISPR-Cas9 gene editing avoiding cellular toxicity
    Article Snippet: Paragraph title: Supporting information Sequences of synthetic sgRNA, crRNA and tracrRNA, including modification locations. PCR primers for amplification of gRNA target sites from genomic DNA for DNA mismatch detection assay. Unmodified synthetic sgRNA require a sequential electroporation method with Cas9 mRNA for efficient gene editing. Cas9 protein levels are detectable 4 hours after electroporation and are similar regardless of electroporation delivery method. MS modified guide RNAs perform similarly to unmodified in a stably expressing Cas9 cell line with some toxicity observed at higher concentrations. ... Membranes were washed and secondary antibody [goat anti-mouse IgG (H+L) Secondary Antibody, HRP conjugate (Thermo Scientific, Cat #32430))] was diluted 1:20,000 in SuperBlock (PBS formulation) with 0.5% Tween20 and incubated with membranes for 2 hours at room temperature.

    Stable Transfection:

    Article Title: Minimal 2'-O-methyl phosphorothioate linkage modification pattern of synthetic guide RNAs for increased stability and efficient CRISPR-Cas9 gene editing avoiding cellular toxicity
    Article Snippet: Paragraph title: Supporting information Sequences of synthetic sgRNA, crRNA and tracrRNA, including modification locations. PCR primers for amplification of gRNA target sites from genomic DNA for DNA mismatch detection assay. Unmodified synthetic sgRNA require a sequential electroporation method with Cas9 mRNA for efficient gene editing. Cas9 protein levels are detectable 4 hours after electroporation and are similar regardless of electroporation delivery method. MS modified guide RNAs perform similarly to unmodified in a stably expressing Cas9 cell line with some toxicity observed at higher concentrations. ... Membranes were washed and secondary antibody [goat anti-mouse IgG (H+L) Secondary Antibody, HRP conjugate (Thermo Scientific, Cat #32430))] was diluted 1:20,000 in SuperBlock (PBS formulation) with 0.5% Tween20 and incubated with membranes for 2 hours at room temperature.

    Blocking Assay:

    Article Title: Production and evaluation of parathyroid hormone receptor1 ligands with intrinsic or assembled peroxidase domains
    Article Snippet: .. Then, the cells were incubated 30 minutes with a HBSS solution containing 1% BSA to block the non-specific binding sites before being incubated with a horse radish peroxidase conjugate of streptavidin (SA-HRP; Invitrogen) for 1 hour. ..

    Article Title: Polyclonal Antibody Production for Membrane Proteins via Genetic Immunization
    Article Snippet: Blocking was done at 37 °C for 1 h in 200 μL/well of TBST containing 3% BSA (heat shock fraction, pH 7, ≥98%; Sigma #A7906-500 G) followed by washing as above. .. Wells were incubated at 37 °C for 1 h in 100 μL/well of secondary antibody (goat anti-mouse IgG [H+L], HRP conjugate; Life Technologies #626520) at a 1:3000 dilution in TBST containing 3% BSA, followed by washing as above.

    Article Title: Formulation of the bivalent prostate cancer vaccine with surgifoam elicits antigen-specific effector T cells in PSA-transgenic mice
    Article Snippet: The tissue samples were washed with phosphate buffered saline (PBS) and incubated with 2% bovine serum albumin for 30 minutes to block the non-specific antigen-antibody immunoreactivity. .. Next, the slides were treated with broad spectrum secondary antibody (Invitrogen) for 30 minutes, and HRP- conjugate (Invitrogen) was then added for another 30 minutes and developed with DAB according to the manufacturer’s instructions (Invitrogen).

    Article Title: Study of the Effects of Betaine and/or C-Phycocyanin on the Growth of Lung Cancer A549 Cells In Vitro and In Vivo
    Article Snippet: After visualisation of p38 MAPK, the membranes were incubated twice for 10 min with in-house dehybridization buffer, twice for 10 min with PBS, and twice for 5 min with TBST, and, after, the protocol was restarted from the blocking of the membrane for the final detection of the β -actin (incubation with the primary antibody was during 6 h on +4°C). .. The secondary antibody used was goat anti-rabbit IgG (H+L), HRP conjugate (#32460, Thermo Scientific) 1/1000.

    Article Title: Altered expression of neuropeptides in FoxG1-null heterozygous mutant mice
    Article Snippet: Forty-micrometer coronal sections were quenched for endogenous peroxidase activity with 3% H2 O2 for 30 min at room temperature, washed in PBS, incubated in blocking solution (10% goat serum, 0.1% Triton in PBS) for 1 h and incubated overnight with primary antibodies (1:100 dilution) at 4 °C. .. The following day, sections were incubated with Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Thermo Scientific, Waltham, MA, USA) for 1 h and stained with the VECTASTAIN Elite ABC system kit (Vector Labs, Burlingame, CA, USA).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Fecal estrogen, progestagen and glucocorticoid metabolites during the estrous cycle and pregnancy in the giant anteater (Myrmecophaga tridactyla): evidence for delayed implantation
    Article Snippet: Fecal extracts were diluted in enzyme immunoassay buffer (EIA buffer; 0.1 M phosphate–buffered saline containing 0.1% bovine serum albumin) prior to assay development to ensure 20–80% total binding. .. Azino–bis–3–ethyl benzthiazoline–6–sulfonic acid (40 mM, ABTS) was used as the substrate and hydrogen peroxide as the catalyst to detect the percent of hormone specific–HRP conjugate bound to the antibody using a MRX Revelation microplate reader (ThermoScientific, Rochester, NY).

    Article Title: Polyclonal Antibody Production for Membrane Proteins via Genetic Immunization
    Article Snippet: For enzyme-linked immunosorbent assays (ELISA), 100 ng of protein from IVT-HMB was coated in wells overnight at 4 °C using 100 μL per well in 0.03 M NaHCO3 , 0.02 M Na2 CO3 , and 96-well flat-bottom MaxiSorp plates (Nunc #439454). .. Wells were incubated at 37 °C for 1 h in 100 μL/well of secondary antibody (goat anti-mouse IgG [H+L], HRP conjugate; Life Technologies #626520) at a 1:3000 dilution in TBST containing 3% BSA, followed by washing as above.

    Article Title: Impact of feline AIM on the susceptibility of cats to renal disease
    Article Snippet: PAC-11 was used for immunoblotting in reducing conditions, whereas #33 and #56 were used for immunoblotting in non-reducing conditions, immunohistochemistry, and ELISA. .. In addition, goat anti-rabbit IgG (H+L) secondary antibody, HRP conjugate (Thermo Fisher Scientific), were used.

    Article Title: The structural basis for inhibition of the classical and lectin complement pathways by S. aureus extracellular adherence protein
    Article Snippet: In order to obtain IC50 values for each inhibitor, a two‐fold dilution series was done by diluting the protein 1:1 in LP buffer (50 m M HEPES (pH 7.5), 140 m M NaCl, 0.1% (w/v) Gelatin, 0.1% (w/v) BSA, 2 m M CaCl2 , 0.5 m M MgCl2 , 1% (v/v) Pooled Complement Human Serum [Innovative Research Inc.]) before direct application to the coated, blocked ELISA plate and incubated for 1 h at 37°C. .. Deposited C3b and C5b‐9 were detected with 0.333 μg/mL C3d Antibody (003–05): sc‐58928 and 0.2 μg/mL C5b‐9 (aE11): sc‐58935 (Santa Cruz Biotechnology, Inc.), respectively, diluted in PBS (pH 7.4), 0.1% (w/v) BSA, 0.05% (v/v) Tween‐20 and incubated at RT for 0.5 h. Finally, the Goat anti‐Mouse IgG, IgM (H + L) Cross Absorbed Secondary Antibody, HRP conjugate (Thermo Scientific) was diluted to 1.6 μg/mL in equivalent dilution buffer and added to each well for 0.5 h at RT.

    Article Title: Extracellular DNA and lipoteichoic acids interact with exopolysaccharides in the extracellular matrix of Streptococcus mutans biofilms
    Article Snippet: Next, 100 μl of conjugated secondary antibody [goat anti-mouse IgG (H+L), HRP conjugate, Invitrogen; diluted 1:2,000] were added to each well, with incubation for 2 h at room temperature. .. Finally, 100 μl of 30% hydrogen peroxide were added to each well, the plates were incubated at room temperature for 4 h, and the absorbance was measured at 405 nm on an ELISA plate reader (Biochrom Ez read 400, Cambourne, UK).

    Incubation:

    Article Title: Production and evaluation of parathyroid hormone receptor1 ligands with intrinsic or assembled peroxidase domains
    Article Snippet: .. Then, the cells were incubated 30 minutes with a HBSS solution containing 1% BSA to block the non-specific binding sites before being incubated with a horse radish peroxidase conjugate of streptavidin (SA-HRP; Invitrogen) for 1 hour. ..

    Article Title: Polyclonal Antibody Production for Membrane Proteins via Genetic Immunization
    Article Snippet: .. Wells were incubated at 37 °C for 1 h in 100 μL/well of secondary antibody (goat anti-mouse IgG [H+L], HRP conjugate; Life Technologies #626520) at a 1:3000 dilution in TBST containing 3% BSA, followed by washing as above. .. Detection was done by addition of 100 μL per well of ABTS (2,2′-azino-di[3-ethyl-benzthiazoline-6-sulfonate]) Peroxidase Substrate, 1-Component (KPL #50-66-06), incubation at 37 °C for 30 min, addition of 100 μL per well of 0.5 M HCl, and measurement at 415 nm in a Molecular Devices SpectraMax 190 plate reader.

    Article Title: Differential modulatory effects of GSK-3? and HDM2 on sorafenib-induced AIF nuclear translocation (programmed necrosis) in melanoma
    Article Snippet: .. The blots were probed for the proteins of interest with specific antibodies followed by a second antibody-horse radish peroxidase conjugate and then incubated with SuperSignal chemiluminescence substrate (Pierce, Rochford, IL). ..

    Article Title: Effects of HDM2 antagonism on sunitinib resistance, p53 activation, SDF-1 induction, and tumor infiltration by CD11b+/Gr-1+ myeloid derived suppressor cells
    Article Snippet: .. The blots were probed for the proteins of interest with specific antibodies followed by a second antibody-horse radish peroxidase conjugate and then incubated with SuperSignal chemiluminescence substrate (Pierce, Rochford, IL). ..

    Article Title: Formulation of the bivalent prostate cancer vaccine with surgifoam elicits antigen-specific effector T cells in PSA-transgenic mice
    Article Snippet: The tissue samples were incubated with 1:50 dilution of anti-PSA antibody overnight at 40°C. .. Next, the slides were treated with broad spectrum secondary antibody (Invitrogen) for 30 minutes, and HRP- conjugate (Invitrogen) was then added for another 30 minutes and developed with DAB according to the manufacturer’s instructions (Invitrogen).

    Article Title: Study of the Effects of Betaine and/or C-Phycocyanin on the Growth of Lung Cancer A549 Cells In Vitro and In Vivo
    Article Snippet: After visualisation of p38 MAPK, the membranes were incubated twice for 10 min with in-house dehybridization buffer, twice for 10 min with PBS, and twice for 5 min with TBST, and, after, the protocol was restarted from the blocking of the membrane for the final detection of the β -actin (incubation with the primary antibody was during 6 h on +4°C). .. The secondary antibody used was goat anti-rabbit IgG (H+L), HRP conjugate (#32460, Thermo Scientific) 1/1000.

    Article Title: Altered expression of neuropeptides in FoxG1-null heterozygous mutant mice
    Article Snippet: .. The following day, sections were incubated with Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Thermo Scientific, Waltham, MA, USA) for 1 h and stained with the VECTASTAIN Elite ABC system kit (Vector Labs, Burlingame, CA, USA). .. Differences in mRNA levels determined in qPCR were assessed by Student's t -test with a significance level of 95%.

    Article Title: Minimal 2'-O-methyl phosphorothioate linkage modification pattern of synthetic guide RNAs for increased stability and efficient CRISPR-Cas9 gene editing avoiding cellular toxicity
    Article Snippet: .. Membranes were washed and secondary antibody [goat anti-mouse IgG (H+L) Secondary Antibody, HRP conjugate (Thermo Scientific, Cat #32430))] was diluted 1:20,000 in SuperBlock (PBS formulation) with 0.5% Tween20 and incubated with membranes for 2 hours at room temperature. .. The membranes were washed and then submerged in SuperSignal™ West Dura Substrate (Thermo Scientific, Cat #34016) solution for beta-actin blots and Super West Femto Maximum Sensitivity Substrate (Thermo Scientific, Cat #34095) for Cas9, and exposed to film. (TIF) Click here for additional data file.

    Article Title: The structural basis for inhibition of the classical and lectin complement pathways by S. aureus extracellular adherence protein
    Article Snippet: .. Deposited C3b and C5b‐9 were detected with 0.333 μg/mL C3d Antibody (003–05): sc‐58928 and 0.2 μg/mL C5b‐9 (aE11): sc‐58935 (Santa Cruz Biotechnology, Inc.), respectively, diluted in PBS (pH 7.4), 0.1% (w/v) BSA, 0.05% (v/v) Tween‐20 and incubated at RT for 0.5 h. Finally, the Goat anti‐Mouse IgG, IgM (H + L) Cross Absorbed Secondary Antibody, HRP conjugate (Thermo Scientific) was diluted to 1.6 μg/mL in equivalent dilution buffer and added to each well for 0.5 h at RT. .. HRP‐labeled Abs were detected with 50 μL of 1‐Step Ultra TMB‐ELISA (Thermo Scientific), the reaction was stopped by the addition of an equal volume of 2 M H2 SO4 , and the absorbance at 450 nm was measured using a VERSAMAX microplate reader.

    Article Title: Extracellular DNA and lipoteichoic acids interact with exopolysaccharides in the extracellular matrix of Streptococcus mutans biofilms
    Article Snippet: .. Next, 100 μl of conjugated secondary antibody [goat anti-mouse IgG (H+L), HRP conjugate, Invitrogen; diluted 1:2,000] were added to each well, with incubation for 2 h at room temperature. ..

    Article Title: The effect of within-instar development on tracheal diameter and hypoxia-inducible factors α and β in the tobacco hornworm, Manduca sexta
    Article Snippet: .. Then, the membrane was washed 3 times for 5 min with TBS-T and incubated with goat anti-rabbit IgG secondary antibody with HRP conjugate (Thermo Scientific, Rockford, IL, USA) at a 1:5,000 dilution (8 µl of secondary antibody in 40 ml of 5% TBS-T and 2 g of dry milk), for 1 hour on a rocker. .. After 1 hour, the membrane was washed 3 times for 10 min with 1X TBS-T. Proteins were visualized using the West-Femto kit (ThermoFisher, Grand Island, NY, USA) with exposure times of 20 – 30 min on a chemi-imager (Alpha-Innotech, Miami, FL, USA).

    Activity Assay:

    Article Title: Altered expression of neuropeptides in FoxG1-null heterozygous mutant mice
    Article Snippet: Forty-micrometer coronal sections were quenched for endogenous peroxidase activity with 3% H2 O2 for 30 min at room temperature, washed in PBS, incubated in blocking solution (10% goat serum, 0.1% Triton in PBS) for 1 h and incubated overnight with primary antibodies (1:100 dilution) at 4 °C. .. The following day, sections were incubated with Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Thermo Scientific, Waltham, MA, USA) for 1 h and stained with the VECTASTAIN Elite ABC system kit (Vector Labs, Burlingame, CA, USA).

    Article Title: The structural basis for inhibition of the classical and lectin complement pathways by S. aureus extracellular adherence protein
    Article Snippet: Paragraph title: Activity of the lectin pathway of complement on an artificial surface ... Deposited C3b and C5b‐9 were detected with 0.333 μg/mL C3d Antibody (003–05): sc‐58928 and 0.2 μg/mL C5b‐9 (aE11): sc‐58935 (Santa Cruz Biotechnology, Inc.), respectively, diluted in PBS (pH 7.4), 0.1% (w/v) BSA, 0.05% (v/v) Tween‐20 and incubated at RT for 0.5 h. Finally, the Goat anti‐Mouse IgG, IgM (H + L) Cross Absorbed Secondary Antibody, HRP conjugate (Thermo Scientific) was diluted to 1.6 μg/mL in equivalent dilution buffer and added to each well for 0.5 h at RT.

    Expressing:

    Article Title: A myosin-Va tail fragment sequesters dynein light chains leading to apoptosis in melanoma cells
    Article Snippet: .. Caspase-9 detection Cell lysates from B16-F10 cells expressing either EGFP-MVaf1 or EGFP were collected at 24, 36, 48 and 60 h post transfection, combined, electrophoresed on 15% SDS-PAGE gel and measured by western blotting for detection of pro-caspase-9 and caspase-9, using pAb anti-caspase-9 (BD Biosciences, catalog no. 552036) and goat anti-rabbit IgG, HRP-conjugate (Pierce, Rockford, IL, USA) as secondary antibody. .. Quantification of the specific bands from the western blotting was measured by the ratio of pixel intensity using ImageJ gel analysis software (U. S. National Institutes of Health, Bethesda, MD, USA).

    Article Title: Minimal 2'-O-methyl phosphorothioate linkage modification pattern of synthetic guide RNAs for increased stability and efficient CRISPR-Cas9 gene editing avoiding cellular toxicity
    Article Snippet: Paragraph title: Supporting information Sequences of synthetic sgRNA, crRNA and tracrRNA, including modification locations. PCR primers for amplification of gRNA target sites from genomic DNA for DNA mismatch detection assay. Unmodified synthetic sgRNA require a sequential electroporation method with Cas9 mRNA for efficient gene editing. Cas9 protein levels are detectable 4 hours after electroporation and are similar regardless of electroporation delivery method. MS modified guide RNAs perform similarly to unmodified in a stably expressing Cas9 cell line with some toxicity observed at higher concentrations. ... Membranes were washed and secondary antibody [goat anti-mouse IgG (H+L) Secondary Antibody, HRP conjugate (Thermo Scientific, Cat #32430))] was diluted 1:20,000 in SuperBlock (PBS formulation) with 0.5% Tween20 and incubated with membranes for 2 hours at room temperature.

    Modification:

    Article Title: Hepatitis C Virus Infects Rhesus Macaque Hepatocytes and Simianized Mice
    Article Snippet: Cells were lysed at the indicated times using modified RIPA buffer containing 50mM Tris-HCl pH 8.0, 1% v/v NP-40, 0.5% v/w Na-deoxycholate, 150mM NaCl, 0.1% SDS. .. Following secondary antibody staining with Peroxidase-AffiniPure Donkey Anti-Mouse IgG (H+L) (Jackson Immuno Research; 1:10000) or goat anti-rabbit IgG (H+L), HRP conjugate (Pierce; 1:10000), western blots were visualized using SuperSignal West Dura, Pico or Femto (Thermo Scientific).

    Article Title: Minimal 2'-O-methyl phosphorothioate linkage modification pattern of synthetic guide RNAs for increased stability and efficient CRISPR-Cas9 gene editing avoiding cellular toxicity
    Article Snippet: Paragraph title: Supporting information Sequences of synthetic sgRNA, crRNA and tracrRNA, including modification locations. PCR primers for amplification of gRNA target sites from genomic DNA for DNA mismatch detection assay. Unmodified synthetic sgRNA require a sequential electroporation method with Cas9 mRNA for efficient gene editing. Cas9 protein levels are detectable 4 hours after electroporation and are similar regardless of electroporation delivery method. MS modified guide RNAs perform similarly to unmodified in a stably expressing Cas9 cell line with some toxicity observed at higher concentrations. ... Membranes were washed and secondary antibody [goat anti-mouse IgG (H+L) Secondary Antibody, HRP conjugate (Thermo Scientific, Cat #32430))] was diluted 1:20,000 in SuperBlock (PBS formulation) with 0.5% Tween20 and incubated with membranes for 2 hours at room temperature.

    Western Blot:

    Article Title: A myosin-Va tail fragment sequesters dynein light chains leading to apoptosis in melanoma cells
    Article Snippet: .. Caspase-9 detection Cell lysates from B16-F10 cells expressing either EGFP-MVaf1 or EGFP were collected at 24, 36, 48 and 60 h post transfection, combined, electrophoresed on 15% SDS-PAGE gel and measured by western blotting for detection of pro-caspase-9 and caspase-9, using pAb anti-caspase-9 (BD Biosciences, catalog no. 552036) and goat anti-rabbit IgG, HRP-conjugate (Pierce, Rockford, IL, USA) as secondary antibody. .. Quantification of the specific bands from the western blotting was measured by the ratio of pixel intensity using ImageJ gel analysis software (U. S. National Institutes of Health, Bethesda, MD, USA).

    Article Title: Hepatitis C Virus Infects Rhesus Macaque Hepatocytes and Simianized Mice
    Article Snippet: .. Following secondary antibody staining with Peroxidase-AffiniPure Donkey Anti-Mouse IgG (H+L) (Jackson Immuno Research; 1:10000) or goat anti-rabbit IgG (H+L), HRP conjugate (Pierce; 1:10000), western blots were visualized using SuperSignal West Dura, Pico or Femto (Thermo Scientific). .. FAH−/− NOD Rag1−/− IL2RNULL mice were generated and transplanted as previously described ( ) Female FNRG mice greater than 6 weeks of age were transplanted with ca.

    Article Title: Differential modulatory effects of GSK-3? and HDM2 on sorafenib-induced AIF nuclear translocation (programmed necrosis) in melanoma
    Article Snippet: Paragraph title: Western blots ... The blots were probed for the proteins of interest with specific antibodies followed by a second antibody-horse radish peroxidase conjugate and then incubated with SuperSignal chemiluminescence substrate (Pierce, Rochford, IL).

    Article Title: Effects of HDM2 antagonism on sunitinib resistance, p53 activation, SDF-1 induction, and tumor infiltration by CD11b+/Gr-1+ myeloid derived suppressor cells
    Article Snippet: Paragraph title: Western blots ... The blots were probed for the proteins of interest with specific antibodies followed by a second antibody-horse radish peroxidase conjugate and then incubated with SuperSignal chemiluminescence substrate (Pierce, Rochford, IL).

    Article Title: Study of the Effects of Betaine and/or C-Phycocyanin on the Growth of Lung Cancer A549 Cells In Vitro and In Vivo
    Article Snippet: Paragraph title: 2.1.5. Western Blot ... The secondary antibody used was goat anti-rabbit IgG (H+L), HRP conjugate (#32460, Thermo Scientific) 1/1000.

    Article Title: The effect of within-instar development on tracheal diameter and hypoxia-inducible factors α and β in the tobacco hornworm, Manduca sexta
    Article Snippet: Paragraph title: 2.3.4 Western blotting ... Then, the membrane was washed 3 times for 5 min with TBS-T and incubated with goat anti-rabbit IgG secondary antibody with HRP conjugate (Thermo Scientific, Rockford, IL, USA) at a 1:5,000 dilution (8 µl of secondary antibody in 40 ml of 5% TBS-T and 2 g of dry milk), for 1 hour on a rocker.

    Derivative Assay:

    Article Title: Extracellular DNA and lipoteichoic acids interact with exopolysaccharides in the extracellular matrix of Streptococcus mutans biofilms
    Article Snippet: Briefly, the antigens (LTA samples from either the standard curve or derived from the biofilm matrix) were used to coat microplates (at 4°C for 24 h). .. Next, 100 μl of conjugated secondary antibody [goat anti-mouse IgG (H+L), HRP conjugate, Invitrogen; diluted 1:2,000] were added to each well, with incubation for 2 h at room temperature.

    Electroporation:

    Article Title: Minimal 2'-O-methyl phosphorothioate linkage modification pattern of synthetic guide RNAs for increased stability and efficient CRISPR-Cas9 gene editing avoiding cellular toxicity
    Article Snippet: Paragraph title: Supporting information Sequences of synthetic sgRNA, crRNA and tracrRNA, including modification locations. PCR primers for amplification of gRNA target sites from genomic DNA for DNA mismatch detection assay. Unmodified synthetic sgRNA require a sequential electroporation method with Cas9 mRNA for efficient gene editing. Cas9 protein levels are detectable 4 hours after electroporation and are similar regardless of electroporation delivery method. MS modified guide RNAs perform similarly to unmodified in a stably expressing Cas9 cell line with some toxicity observed at higher concentrations. ... Membranes were washed and secondary antibody [goat anti-mouse IgG (H+L) Secondary Antibody, HRP conjugate (Thermo Scientific, Cat #32430))] was diluted 1:20,000 in SuperBlock (PBS formulation) with 0.5% Tween20 and incubated with membranes for 2 hours at room temperature.

    Transfection:

    Article Title: A myosin-Va tail fragment sequesters dynein light chains leading to apoptosis in melanoma cells
    Article Snippet: .. Caspase-9 detection Cell lysates from B16-F10 cells expressing either EGFP-MVaf1 or EGFP were collected at 24, 36, 48 and 60 h post transfection, combined, electrophoresed on 15% SDS-PAGE gel and measured by western blotting for detection of pro-caspase-9 and caspase-9, using pAb anti-caspase-9 (BD Biosciences, catalog no. 552036) and goat anti-rabbit IgG, HRP-conjugate (Pierce, Rockford, IL, USA) as secondary antibody. .. Quantification of the specific bands from the western blotting was measured by the ratio of pixel intensity using ImageJ gel analysis software (U. S. National Institutes of Health, Bethesda, MD, USA).

    Article Title: Minimal 2'-O-methyl phosphorothioate linkage modification pattern of synthetic guide RNAs for increased stability and efficient CRISPR-Cas9 gene editing avoiding cellular toxicity
    Article Snippet: Membranes were washed and secondary antibody [goat anti-mouse IgG (H+L) Secondary Antibody, HRP conjugate (Thermo Scientific, Cat #32430))] was diluted 1:20,000 in SuperBlock (PBS formulation) with 0.5% Tween20 and incubated with membranes for 2 hours at room temperature. .. Gene editing efficiency of unmodified (unmod) and modified crRNA:tracrRNA or sgRNA showed similar levels of gene editing efficiencies ( < 1.5-fold difference) measured by EGFP fluorescence from knockout of a proteasome component, PSMD7 ( A. ) or PSMD11 ( B.), at multiple concentrations when transfected at 1.5625 nM to 50 nM at 2-fold increments into a stably expressing Cas9 U2OS cell line.

    Generated:

    Article Title: Impact of feline AIM on the susceptibility of cats to renal disease
    Article Snippet: Antibodies for cats were generated by immunizing mice (monoclonal) or rabbits (polyclonal) with feline AIM recombinant proteins tagged with an HA peptide. .. In addition, goat anti-rabbit IgG (H+L) secondary antibody, HRP conjugate (Thermo Fisher Scientific), were used.

    Polymerase Chain Reaction:

    Article Title: Minimal 2'-O-methyl phosphorothioate linkage modification pattern of synthetic guide RNAs for increased stability and efficient CRISPR-Cas9 gene editing avoiding cellular toxicity
    Article Snippet: Paragraph title: Supporting information Sequences of synthetic sgRNA, crRNA and tracrRNA, including modification locations. PCR primers for amplification of gRNA target sites from genomic DNA for DNA mismatch detection assay. Unmodified synthetic sgRNA require a sequential electroporation method with Cas9 mRNA for efficient gene editing. Cas9 protein levels are detectable 4 hours after electroporation and are similar regardless of electroporation delivery method. MS modified guide RNAs perform similarly to unmodified in a stably expressing Cas9 cell line with some toxicity observed at higher concentrations. ... Membranes were washed and secondary antibody [goat anti-mouse IgG (H+L) Secondary Antibody, HRP conjugate (Thermo Scientific, Cat #32430))] was diluted 1:20,000 in SuperBlock (PBS formulation) with 0.5% Tween20 and incubated with membranes for 2 hours at room temperature.

    Injection:

    Article Title: Altered expression of neuropeptides in FoxG1-null heterozygous mutant mice
    Article Snippet: Mice were intraperitoneally injected with 200 mg/kg avertin and transcardially perfused with 0.9% saline, followed by ice-cold 4% PFA (Sigma-Aldrich). .. The following day, sections were incubated with Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Thermo Scientific, Waltham, MA, USA) for 1 h and stained with the VECTASTAIN Elite ABC system kit (Vector Labs, Burlingame, CA, USA).

    Recombinant:

    Article Title: Impact of feline AIM on the susceptibility of cats to renal disease
    Article Snippet: Antibodies for cats were generated by immunizing mice (monoclonal) or rabbits (polyclonal) with feline AIM recombinant proteins tagged with an HA peptide. .. In addition, goat anti-rabbit IgG (H+L) secondary antibody, HRP conjugate (Thermo Fisher Scientific), were used.

    Nucleic Acid Electrophoresis:

    Article Title: Minimal 2'-O-methyl phosphorothioate linkage modification pattern of synthetic guide RNAs for increased stability and efficient CRISPR-Cas9 gene editing avoiding cellular toxicity
    Article Snippet: NuPAGETM 4X LDS sample buffer and NuPAGETM Sample Reducing Agent (10X) (Life Technologies, Cat #NP0008, # NP0009) were added to samples before gel electrophoresis. .. Membranes were washed and secondary antibody [goat anti-mouse IgG (H+L) Secondary Antibody, HRP conjugate (Thermo Scientific, Cat #32430))] was diluted 1:20,000 in SuperBlock (PBS formulation) with 0.5% Tween20 and incubated with membranes for 2 hours at room temperature.

    Enzyme Immunoassay:

    Article Title: Fecal estrogen, progestagen and glucocorticoid metabolites during the estrous cycle and pregnancy in the giant anteater (Myrmecophaga tridactyla): evidence for delayed implantation
    Article Snippet: Paragraph title: Enzyme immunoassays ... Azino–bis–3–ethyl benzthiazoline–6–sulfonic acid (40 mM, ABTS) was used as the substrate and hydrogen peroxide as the catalyst to detect the percent of hormone specific–HRP conjugate bound to the antibody using a MRX Revelation microplate reader (ThermoScientific, Rochester, NY).

    Fluorescence:

    Article Title: Minimal 2'-O-methyl phosphorothioate linkage modification pattern of synthetic guide RNAs for increased stability and efficient CRISPR-Cas9 gene editing avoiding cellular toxicity
    Article Snippet: Membranes were washed and secondary antibody [goat anti-mouse IgG (H+L) Secondary Antibody, HRP conjugate (Thermo Scientific, Cat #32430))] was diluted 1:20,000 in SuperBlock (PBS formulation) with 0.5% Tween20 and incubated with membranes for 2 hours at room temperature. .. Gene editing efficiency of unmodified (unmod) and modified crRNA:tracrRNA or sgRNA showed similar levels of gene editing efficiencies ( < 1.5-fold difference) measured by EGFP fluorescence from knockout of a proteasome component, PSMD7 ( A. ) or PSMD11 ( B.), at multiple concentrations when transfected at 1.5625 nM to 50 nM at 2-fold increments into a stably expressing Cas9 U2OS cell line.

    Isolation:

    Article Title: Extracellular DNA and lipoteichoic acids interact with exopolysaccharides in the extracellular matrix of Streptococcus mutans biofilms
    Article Snippet: Paragraph title: Isolation and quantification of LTA ... Next, 100 μl of conjugated secondary antibody [goat anti-mouse IgG (H+L), HRP conjugate, Invitrogen; diluted 1:2,000] were added to each well, with incubation for 2 h at room temperature.

    Detection Assay:

    Article Title: Minimal 2'-O-methyl phosphorothioate linkage modification pattern of synthetic guide RNAs for increased stability and efficient CRISPR-Cas9 gene editing avoiding cellular toxicity
    Article Snippet: Paragraph title: Supporting information Sequences of synthetic sgRNA, crRNA and tracrRNA, including modification locations. PCR primers for amplification of gRNA target sites from genomic DNA for DNA mismatch detection assay. Unmodified synthetic sgRNA require a sequential electroporation method with Cas9 mRNA for efficient gene editing. Cas9 protein levels are detectable 4 hours after electroporation and are similar regardless of electroporation delivery method. MS modified guide RNAs perform similarly to unmodified in a stably expressing Cas9 cell line with some toxicity observed at higher concentrations. ... Membranes were washed and secondary antibody [goat anti-mouse IgG (H+L) Secondary Antibody, HRP conjugate (Thermo Scientific, Cat #32430))] was diluted 1:20,000 in SuperBlock (PBS formulation) with 0.5% Tween20 and incubated with membranes for 2 hours at room temperature.

    Immunohistochemistry:

    Article Title: Impact of feline AIM on the susceptibility of cats to renal disease
    Article Snippet: PAC-11 was used for immunoblotting in reducing conditions, whereas #33 and #56 were used for immunoblotting in non-reducing conditions, immunohistochemistry, and ELISA. .. In addition, goat anti-rabbit IgG (H+L) secondary antibody, HRP conjugate (Thermo Fisher Scientific), were used.

    Article Title: Formulation of the bivalent prostate cancer vaccine with surgifoam elicits antigen-specific effector T cells in PSA-transgenic mice
    Article Snippet: Paragraph title: Immunohistochemistry ... Next, the slides were treated with broad spectrum secondary antibody (Invitrogen) for 30 minutes, and HRP- conjugate (Invitrogen) was then added for another 30 minutes and developed with DAB according to the manufacturer’s instructions (Invitrogen).

    Article Title: Altered expression of neuropeptides in FoxG1-null heterozygous mutant mice
    Article Snippet: Paragraph title: Immunohistochemistry ... The following day, sections were incubated with Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Thermo Scientific, Waltham, MA, USA) for 1 h and stained with the VECTASTAIN Elite ABC system kit (Vector Labs, Burlingame, CA, USA).

    Mouse Assay:

    Article Title: Impact of feline AIM on the susceptibility of cats to renal disease
    Article Snippet: Antibodies for cats were generated by immunizing mice (monoclonal) or rabbits (polyclonal) with feline AIM recombinant proteins tagged with an HA peptide. .. In addition, goat anti-rabbit IgG (H+L) secondary antibody, HRP conjugate (Thermo Fisher Scientific), were used.

    Article Title: Altered expression of neuropeptides in FoxG1-null heterozygous mutant mice
    Article Snippet: Mice were intraperitoneally injected with 200 mg/kg avertin and transcardially perfused with 0.9% saline, followed by ice-cold 4% PFA (Sigma-Aldrich). .. The following day, sections were incubated with Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Thermo Scientific, Waltham, MA, USA) for 1 h and stained with the VECTASTAIN Elite ABC system kit (Vector Labs, Burlingame, CA, USA).

    Staining:

    Article Title: Hepatitis C Virus Infects Rhesus Macaque Hepatocytes and Simianized Mice
    Article Snippet: .. Following secondary antibody staining with Peroxidase-AffiniPure Donkey Anti-Mouse IgG (H+L) (Jackson Immuno Research; 1:10000) or goat anti-rabbit IgG (H+L), HRP conjugate (Pierce; 1:10000), western blots were visualized using SuperSignal West Dura, Pico or Femto (Thermo Scientific). .. FAH−/− NOD Rag1−/− IL2RNULL mice were generated and transplanted as previously described ( ) Female FNRG mice greater than 6 weeks of age were transplanted with ca.

    Article Title: Formulation of the bivalent prostate cancer vaccine with surgifoam elicits antigen-specific effector T cells in PSA-transgenic mice
    Article Snippet: Prostate tissues embedded in paraffin block were cut to a section of 4 μm and stained with monoclonal anti-human PSA antibody (Abcam, Inc.). .. Next, the slides were treated with broad spectrum secondary antibody (Invitrogen) for 30 minutes, and HRP- conjugate (Invitrogen) was then added for another 30 minutes and developed with DAB according to the manufacturer’s instructions (Invitrogen).

    Article Title: Altered expression of neuropeptides in FoxG1-null heterozygous mutant mice
    Article Snippet: .. The following day, sections were incubated with Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Thermo Scientific, Waltham, MA, USA) for 1 h and stained with the VECTASTAIN Elite ABC system kit (Vector Labs, Burlingame, CA, USA). .. Differences in mRNA levels determined in qPCR were assessed by Student's t -test with a significance level of 95%.

    IA:

    Article Title: The effect of within-instar development on tracheal diameter and hypoxia-inducible factors α and β in the tobacco hornworm, Manduca sexta
    Article Snippet: Separated proteins were transferred to nitrocellulose membranes (TransBlot Turbo Kit, Bio-Rad; Hercules, CA, USA) and blocked in 40 ml of 5% dry milk (HyVee, Des Moines, IA, USA) in tris-buffered saline with 0.1% Tween 20 (TBS-T) for 1 hour on a rocking shaker. .. Then, the membrane was washed 3 times for 5 min with TBS-T and incubated with goat anti-rabbit IgG secondary antibody with HRP conjugate (Thermo Scientific, Rockford, IL, USA) at a 1:5,000 dilution (8 µl of secondary antibody in 40 ml of 5% TBS-T and 2 g of dry milk), for 1 hour on a rocker.

    SDS Page:

    Article Title: A myosin-Va tail fragment sequesters dynein light chains leading to apoptosis in melanoma cells
    Article Snippet: .. Caspase-9 detection Cell lysates from B16-F10 cells expressing either EGFP-MVaf1 or EGFP were collected at 24, 36, 48 and 60 h post transfection, combined, electrophoresed on 15% SDS-PAGE gel and measured by western blotting for detection of pro-caspase-9 and caspase-9, using pAb anti-caspase-9 (BD Biosciences, catalog no. 552036) and goat anti-rabbit IgG, HRP-conjugate (Pierce, Rockford, IL, USA) as secondary antibody. .. Quantification of the specific bands from the western blotting was measured by the ratio of pixel intensity using ImageJ gel analysis software (U. S. National Institutes of Health, Bethesda, MD, USA).

    Software:

    Article Title: A myosin-Va tail fragment sequesters dynein light chains leading to apoptosis in melanoma cells
    Article Snippet: Caspase-9 detection Cell lysates from B16-F10 cells expressing either EGFP-MVaf1 or EGFP were collected at 24, 36, 48 and 60 h post transfection, combined, electrophoresed on 15% SDS-PAGE gel and measured by western blotting for detection of pro-caspase-9 and caspase-9, using pAb anti-caspase-9 (BD Biosciences, catalog no. 552036) and goat anti-rabbit IgG, HRP-conjugate (Pierce, Rockford, IL, USA) as secondary antibody. .. Quantification of the specific bands from the western blotting was measured by the ratio of pixel intensity using ImageJ gel analysis software (U. S. National Institutes of Health, Bethesda, MD, USA).

    Article Title: The structural basis for inhibition of the classical and lectin complement pathways by S. aureus extracellular adherence protein
    Article Snippet: Deposited C3b and C5b‐9 were detected with 0.333 μg/mL C3d Antibody (003–05): sc‐58928 and 0.2 μg/mL C5b‐9 (aE11): sc‐58935 (Santa Cruz Biotechnology, Inc.), respectively, diluted in PBS (pH 7.4), 0.1% (w/v) BSA, 0.05% (v/v) Tween‐20 and incubated at RT for 0.5 h. Finally, the Goat anti‐Mouse IgG, IgM (H + L) Cross Absorbed Secondary Antibody, HRP conjugate (Thermo Scientific) was diluted to 1.6 μg/mL in equivalent dilution buffer and added to each well for 0.5 h at RT. .. Data were fit to a four‐parameter (variable slope) dose‐response—inhibition curve software (GraphPad, La Jolla, CA).

    Functional Assay:

    Article Title: The structural basis for inhibition of the classical and lectin complement pathways by S. aureus extracellular adherence protein
    Article Snippet: Functional activity of the lectin pathway (LP) was determined using a previously described method., In short, 96‐well polystyrene high bind microplates (Corning Life Sciences) were coated overnight to specifically activate the LP (20 μg/mL Saccharomyces cerevisiae mannan [Sigma‐Aldrich]). .. Deposited C3b and C5b‐9 were detected with 0.333 μg/mL C3d Antibody (003–05): sc‐58928 and 0.2 μg/mL C5b‐9 (aE11): sc‐58935 (Santa Cruz Biotechnology, Inc.), respectively, diluted in PBS (pH 7.4), 0.1% (w/v) BSA, 0.05% (v/v) Tween‐20 and incubated at RT for 0.5 h. Finally, the Goat anti‐Mouse IgG, IgM (H + L) Cross Absorbed Secondary Antibody, HRP conjugate (Thermo Scientific) was diluted to 1.6 μg/mL in equivalent dilution buffer and added to each well for 0.5 h at RT.

    Binding Assay:

    Article Title: Production and evaluation of parathyroid hormone receptor1 ligands with intrinsic or assembled peroxidase domains
    Article Snippet: .. Then, the cells were incubated 30 minutes with a HBSS solution containing 1% BSA to block the non-specific binding sites before being incubated with a horse radish peroxidase conjugate of streptavidin (SA-HRP; Invitrogen) for 1 hour. ..

    Article Title: Fecal estrogen, progestagen and glucocorticoid metabolites during the estrous cycle and pregnancy in the giant anteater (Myrmecophaga tridactyla): evidence for delayed implantation
    Article Snippet: Hormone conjugated to horseradish peroxidase (HRP) was applied to compete for binding sites on the antibodies, and plates were allowed to equilibrate at room temperature for two hours. .. Azino–bis–3–ethyl benzthiazoline–6–sulfonic acid (40 mM, ABTS) was used as the substrate and hydrogen peroxide as the catalyst to detect the percent of hormone specific–HRP conjugate bound to the antibody using a MRX Revelation microplate reader (ThermoScientific, Rochester, NY).

    Knock-Out:

    Article Title: Minimal 2'-O-methyl phosphorothioate linkage modification pattern of synthetic guide RNAs for increased stability and efficient CRISPR-Cas9 gene editing avoiding cellular toxicity
    Article Snippet: Membranes were washed and secondary antibody [goat anti-mouse IgG (H+L) Secondary Antibody, HRP conjugate (Thermo Scientific, Cat #32430))] was diluted 1:20,000 in SuperBlock (PBS formulation) with 0.5% Tween20 and incubated with membranes for 2 hours at room temperature. .. Gene editing efficiency of unmodified (unmod) and modified crRNA:tracrRNA or sgRNA showed similar levels of gene editing efficiencies ( < 1.5-fold difference) measured by EGFP fluorescence from knockout of a proteasome component, PSMD7 ( A. ) or PSMD11 ( B.), at multiple concentrations when transfected at 1.5625 nM to 50 nM at 2-fold increments into a stably expressing Cas9 U2OS cell line.

    Lysis:

    Article Title: Differential modulatory effects of GSK-3? and HDM2 on sorafenib-induced AIF nuclear translocation (programmed necrosis) in melanoma
    Article Snippet: Western blots Cells were treated as described in Results and then lysed in Lysis Solution (Cell Signaling) supplemented with sodium fluoride (10 μM, Fisher Scientific, Hampton, NH) and phenylmethylsulfonyl fluoride (100 μg/ml, Sigma-Aldrich, St Louis, MO). .. The blots were probed for the proteins of interest with specific antibodies followed by a second antibody-horse radish peroxidase conjugate and then incubated with SuperSignal chemiluminescence substrate (Pierce, Rochford, IL).

    Article Title: Effects of HDM2 antagonism on sunitinib resistance, p53 activation, SDF-1 induction, and tumor infiltration by CD11b+/Gr-1+ myeloid derived suppressor cells
    Article Snippet: Western blots Cells were treated as described in Results and then lysed in Lysis Solution (Cell Signaling) supplemented with sodium fluoride (10 μM, Fisher Scientific, Hampton, NH) and phenylmethylsulfonyl fluoride (100 μg/ml, Sigma-Aldrich, St Louis, MO). .. The blots were probed for the proteins of interest with specific antibodies followed by a second antibody-horse radish peroxidase conjugate and then incubated with SuperSignal chemiluminescence substrate (Pierce, Rochford, IL).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars

  • 78
    Thermo Fisher neu hrp
    Avi-GCPII cleavage by TEV protease Purified Avi-GCPII was mixed with TEV protease in different ratios. The reactions were incubated overnight at 4°C and then analyzed using Western blots. Blots were visualized with the monoclonal antibody GCP-04, which recognizes total Avi-GCPII ( Panel A ), and <t>Neu-HRP,</t> which recognizes only biotinylated Avi-GCPII ( Panel B ). 1. purified Avi-GCPII without TEV protease; 2. equimolar mixture of Avi-GCPII and TEV protease; 3. Avi-GCPII with a 100-fold molar excess of TEV protease; 4. 7 ng (panel A) or 3 ng (Panel B) of Avi-GCPII standard. Lanes 1–3 in both panels contained identical volumes of the analyzed samples.
    Neu Hrp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/neu hrp/product/Thermo Fisher
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    neu hrp - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    99
    Thermo Fisher hrp activity
    Adherence of Y . pseudotuberculosis to HEp-2 cells is reduced by C20. (A) Adherence of Y . pseudotuberculosis to HEp-2 cells in the presence of compounds was determined by ELISA. WT Y . pseudotuberculosis was incubated with 60 μM compound. HEp-2 cells were infected in the presence of compounds with Y . pseudotuberculosis at an MOI of 10:1 for 30 min at 37°C. HEp-2 cells were washed to remove any unbound Y . pseudotuberculosis , fixed with 4% paraformaldehdye, and then probed with antisera to Yersinia . <t>Anti-rabbit-HRP</t> was used to detect anti- Yersinia antibody. The HRP activity was visualized with the <t>TMB</t> ELISA reagent, and the DMSO control was set to 100% adherence. The means and standard deviations for one representative experiment are plotted. (B) WT Y . pseudotuberculosis or adherence mutants were grown in 2× YT supplemented with 5 mM Ca 2+ with C20 or 0.3% DMSO and were used to infect HEp-2 cells. The percent adherence was determined by ELISA as described above. The percent adherence of WT Y . pseudotuberculosis grown in 0.3% DMSO was set to 100%. The means and standard deviations for one representative experiment are plotted. (C) Overnight cultures of Y . pseudotuberculosis were inoculated into RPMI and allowed to incubate statically at 37°C for 3 h in the presence or absence of C20. Autoagglutination was measured as described in Materials and Methods. The percent autoagglutination of WT IP2666 in 0.3% DMSO was set to 100%. The means and standard deviations for one representative experiment are shown. (D) E. coli carrying pDS132 or pDS132- yadA was cultured in 60 μM C20 or in 0.3% DMSO and was used to infect HEp-2 cells in the presence of C20 or DMSO. Adherence of E. coli to HEp-2 cells was determined by ELISA as described for panel A, using antiserum to the E. coli phage λ receptor, LamB. The means and standard deviations for one representative experiment are plotted. (E) Hemolysis of SRBCs by Y . pseudotuberculosis adherence mutants or WT Y . pseudotuberculosis grown in the presence of 60 μM C20. Y . pseudotuberculosis was grown in secretion medium in the presence of either 0.3% DMSO or C20 and was used to infect SRBCs at an MOI of 1:1. The percent hemolysis was measured by the amount of hemoglobin released from the SRBCs (see Materials and Methods). Hemolysis by WT Y . pseudotuberculosis in 0.3% DMSO was set to 100%. The means and standard deviations for one representative experiment are shown.
    Hrp Activity, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 250 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp activity/product/Thermo Fisher
    Average 99 stars, based on 250 article reviews
    Price from $9.99 to $1999.99
    hrp activity - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    Image Search Results


    Avi-GCPII cleavage by TEV protease Purified Avi-GCPII was mixed with TEV protease in different ratios. The reactions were incubated overnight at 4°C and then analyzed using Western blots. Blots were visualized with the monoclonal antibody GCP-04, which recognizes total Avi-GCPII ( Panel A ), and Neu-HRP, which recognizes only biotinylated Avi-GCPII ( Panel B ). 1. purified Avi-GCPII without TEV protease; 2. equimolar mixture of Avi-GCPII and TEV protease; 3. Avi-GCPII with a 100-fold molar excess of TEV protease; 4. 7 ng (panel A) or 3 ng (Panel B) of Avi-GCPII standard. Lanes 1–3 in both panels contained identical volumes of the analyzed samples.

    Journal: Protein expression and purification

    Article Title: Efficient and versatile one-step affinity purification of in vivo biotinylated proteins: Expression, characterization and structure analysis of recombinant human Glutamate Carboxypeptidase II

    doi: 10.1016/j.pep.2011.11.016

    Figure Lengend Snippet: Avi-GCPII cleavage by TEV protease Purified Avi-GCPII was mixed with TEV protease in different ratios. The reactions were incubated overnight at 4°C and then analyzed using Western blots. Blots were visualized with the monoclonal antibody GCP-04, which recognizes total Avi-GCPII ( Panel A ), and Neu-HRP, which recognizes only biotinylated Avi-GCPII ( Panel B ). 1. purified Avi-GCPII without TEV protease; 2. equimolar mixture of Avi-GCPII and TEV protease; 3. Avi-GCPII with a 100-fold molar excess of TEV protease; 4. 7 ng (panel A) or 3 ng (Panel B) of Avi-GCPII standard. Lanes 1–3 in both panels contained identical volumes of the analyzed samples.

    Article Snippet: For detection of biotinylated proteins, Neu-HRP (1 mg/mL) was diluted 1:2500 in casein blocker and incubated with the membrane for 1 hour.

    Techniques: Purification, Incubation, Western Blot

    Adherence of Y . pseudotuberculosis to HEp-2 cells is reduced by C20. (A) Adherence of Y . pseudotuberculosis to HEp-2 cells in the presence of compounds was determined by ELISA. WT Y . pseudotuberculosis was incubated with 60 μM compound. HEp-2 cells were infected in the presence of compounds with Y . pseudotuberculosis at an MOI of 10:1 for 30 min at 37°C. HEp-2 cells were washed to remove any unbound Y . pseudotuberculosis , fixed with 4% paraformaldehdye, and then probed with antisera to Yersinia . Anti-rabbit-HRP was used to detect anti- Yersinia antibody. The HRP activity was visualized with the TMB ELISA reagent, and the DMSO control was set to 100% adherence. The means and standard deviations for one representative experiment are plotted. (B) WT Y . pseudotuberculosis or adherence mutants were grown in 2× YT supplemented with 5 mM Ca 2+ with C20 or 0.3% DMSO and were used to infect HEp-2 cells. The percent adherence was determined by ELISA as described above. The percent adherence of WT Y . pseudotuberculosis grown in 0.3% DMSO was set to 100%. The means and standard deviations for one representative experiment are plotted. (C) Overnight cultures of Y . pseudotuberculosis were inoculated into RPMI and allowed to incubate statically at 37°C for 3 h in the presence or absence of C20. Autoagglutination was measured as described in Materials and Methods. The percent autoagglutination of WT IP2666 in 0.3% DMSO was set to 100%. The means and standard deviations for one representative experiment are shown. (D) E. coli carrying pDS132 or pDS132- yadA was cultured in 60 μM C20 or in 0.3% DMSO and was used to infect HEp-2 cells in the presence of C20 or DMSO. Adherence of E. coli to HEp-2 cells was determined by ELISA as described for panel A, using antiserum to the E. coli phage λ receptor, LamB. The means and standard deviations for one representative experiment are plotted. (E) Hemolysis of SRBCs by Y . pseudotuberculosis adherence mutants or WT Y . pseudotuberculosis grown in the presence of 60 μM C20. Y . pseudotuberculosis was grown in secretion medium in the presence of either 0.3% DMSO or C20 and was used to infect SRBCs at an MOI of 1:1. The percent hemolysis was measured by the amount of hemoglobin released from the SRBCs (see Materials and Methods). Hemolysis by WT Y . pseudotuberculosis in 0.3% DMSO was set to 100%. The means and standard deviations for one representative experiment are shown.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Identification and Characterization of Small-Molecule Inhibitors of Yop Translocation in Yersinia pseudotuberculosis ▿

    doi: 10.1128/AAC.00364-10

    Figure Lengend Snippet: Adherence of Y . pseudotuberculosis to HEp-2 cells is reduced by C20. (A) Adherence of Y . pseudotuberculosis to HEp-2 cells in the presence of compounds was determined by ELISA. WT Y . pseudotuberculosis was incubated with 60 μM compound. HEp-2 cells were infected in the presence of compounds with Y . pseudotuberculosis at an MOI of 10:1 for 30 min at 37°C. HEp-2 cells were washed to remove any unbound Y . pseudotuberculosis , fixed with 4% paraformaldehdye, and then probed with antisera to Yersinia . Anti-rabbit-HRP was used to detect anti- Yersinia antibody. The HRP activity was visualized with the TMB ELISA reagent, and the DMSO control was set to 100% adherence. The means and standard deviations for one representative experiment are plotted. (B) WT Y . pseudotuberculosis or adherence mutants were grown in 2× YT supplemented with 5 mM Ca 2+ with C20 or 0.3% DMSO and were used to infect HEp-2 cells. The percent adherence was determined by ELISA as described above. The percent adherence of WT Y . pseudotuberculosis grown in 0.3% DMSO was set to 100%. The means and standard deviations for one representative experiment are plotted. (C) Overnight cultures of Y . pseudotuberculosis were inoculated into RPMI and allowed to incubate statically at 37°C for 3 h in the presence or absence of C20. Autoagglutination was measured as described in Materials and Methods. The percent autoagglutination of WT IP2666 in 0.3% DMSO was set to 100%. The means and standard deviations for one representative experiment are shown. (D) E. coli carrying pDS132 or pDS132- yadA was cultured in 60 μM C20 or in 0.3% DMSO and was used to infect HEp-2 cells in the presence of C20 or DMSO. Adherence of E. coli to HEp-2 cells was determined by ELISA as described for panel A, using antiserum to the E. coli phage λ receptor, LamB. The means and standard deviations for one representative experiment are plotted. (E) Hemolysis of SRBCs by Y . pseudotuberculosis adherence mutants or WT Y . pseudotuberculosis grown in the presence of 60 μM C20. Y . pseudotuberculosis was grown in secretion medium in the presence of either 0.3% DMSO or C20 and was used to infect SRBCs at an MOI of 1:1. The percent hemolysis was measured by the amount of hemoglobin released from the SRBCs (see Materials and Methods). Hemolysis by WT Y . pseudotuberculosis in 0.3% DMSO was set to 100%. The means and standard deviations for one representative experiment are shown.

    Article Snippet: The HRP activity was visualized with the TMB ELISA reagent (Thermoscientific) and was measured spectrophotometrically by determining the OD450 .

    Techniques: Enzyme-linked Immunosorbent Assay, Incubation, Infection, Activity Assay, Cell Culture