hrp conjugate  (Thermo Fisher)


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  • 99
    Name:
    Horseradish Peroxidase HRP EIA Grade
    Description:
    This highly purified preparation of horseradish peroxidase is useful as a protein tracer in histochemistry as well as cytochemistry and also as a valuable experimental tool in hodological neurography Also the enzyme preparation has been used as an enzyme label in enzyme immunoassays
    Catalog Number:
    012001
    Price:
    None
    Applications:
    Cell Analysis|Cellular Imaging|IHC Staining & Detection|Immunocytochemistry (ICC)|Immunofluorescence (IF)|Immunohistochemistry (IHC)|Labeling Proteins & Antibodies with Enzymes & Proteins|Protein Biology|Protein Labeling|Protein Labeling & Crosslinking|Protein, Peptide & Antibody Labeling|Labeling Chemistry
    Category:
    Antibodies Secondary Detection Reagents
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    Structured Review

    Thermo Fisher hrp conjugate
    Determination of the presence of EF-Tu, l -asparaginase II and OmpT in outer membrane protein (OMP) extracts and culture supernatants. Protein extracts were separated by SDS-PAGE (12% acrylamide) and analyzed by Western blot assay. Arrows indicate the detection of the corresponding protein. ( A ) Identification of EF-Tu in OMPs and culture supernatant of the EDL933 strain. Monoclonal anti-EF-Tu antibody (mAb 900) was used in a dilution of 1:2000, followed by anti-mouse <t>IgG,</t> <t>HRP</t> conjugate diluted 1:5000. ( B ) Identification of l -asparaginase II in the OMP extracts and culture supernatant obtained from the EDL933, the isogenic mutant ELD933∆ ansB and the complemented ELD933∆ ansB /pVB1_ ansB strains. Anti- l -asparaginase II antibody was used in a dilution of 1:2000, followed by anti-rabbit IgG, HRP conjugate diluted 1:5000. ( C ) Identification of OmpT in the OMP extracts and culture supernatant obtained from the EDL933, the isogenic mutant ELD933∆ ompT and the complemented ELD933∆ ompT /pVB1_ ompT strains. Anti-OmpT antibody was used in a dilution of 1:3000, followed by anti-rabbit IgG, HRP conjugate diluted 1:5000.
    This highly purified preparation of horseradish peroxidase is useful as a protein tracer in histochemistry as well as cytochemistry and also as a valuable experimental tool in hodological neurography Also the enzyme preparation has been used as an enzyme label in enzyme immunoassays
    https://www.bioz.com/result/hrp conjugate/product/Thermo Fisher
    Average 99 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    hrp conjugate - by Bioz Stars, 2020-10
    99/100 stars

    Images

    1) Product Images from "Deciphering Additional Roles for the EF-Tu, l-Asparaginase II and OmpT Proteins of Shiga Toxin-Producing Escherichia coli"

    Article Title: Deciphering Additional Roles for the EF-Tu, l-Asparaginase II and OmpT Proteins of Shiga Toxin-Producing Escherichia coli

    Journal: Microorganisms

    doi: 10.3390/microorganisms8081184

    Determination of the presence of EF-Tu, l -asparaginase II and OmpT in outer membrane protein (OMP) extracts and culture supernatants. Protein extracts were separated by SDS-PAGE (12% acrylamide) and analyzed by Western blot assay. Arrows indicate the detection of the corresponding protein. ( A ) Identification of EF-Tu in OMPs and culture supernatant of the EDL933 strain. Monoclonal anti-EF-Tu antibody (mAb 900) was used in a dilution of 1:2000, followed by anti-mouse IgG, HRP conjugate diluted 1:5000. ( B ) Identification of l -asparaginase II in the OMP extracts and culture supernatant obtained from the EDL933, the isogenic mutant ELD933∆ ansB and the complemented ELD933∆ ansB /pVB1_ ansB strains. Anti- l -asparaginase II antibody was used in a dilution of 1:2000, followed by anti-rabbit IgG, HRP conjugate diluted 1:5000. ( C ) Identification of OmpT in the OMP extracts and culture supernatant obtained from the EDL933, the isogenic mutant ELD933∆ ompT and the complemented ELD933∆ ompT /pVB1_ ompT strains. Anti-OmpT antibody was used in a dilution of 1:3000, followed by anti-rabbit IgG, HRP conjugate diluted 1:5000.
    Figure Legend Snippet: Determination of the presence of EF-Tu, l -asparaginase II and OmpT in outer membrane protein (OMP) extracts and culture supernatants. Protein extracts were separated by SDS-PAGE (12% acrylamide) and analyzed by Western blot assay. Arrows indicate the detection of the corresponding protein. ( A ) Identification of EF-Tu in OMPs and culture supernatant of the EDL933 strain. Monoclonal anti-EF-Tu antibody (mAb 900) was used in a dilution of 1:2000, followed by anti-mouse IgG, HRP conjugate diluted 1:5000. ( B ) Identification of l -asparaginase II in the OMP extracts and culture supernatant obtained from the EDL933, the isogenic mutant ELD933∆ ansB and the complemented ELD933∆ ansB /pVB1_ ansB strains. Anti- l -asparaginase II antibody was used in a dilution of 1:2000, followed by anti-rabbit IgG, HRP conjugate diluted 1:5000. ( C ) Identification of OmpT in the OMP extracts and culture supernatant obtained from the EDL933, the isogenic mutant ELD933∆ ompT and the complemented ELD933∆ ompT /pVB1_ ompT strains. Anti-OmpT antibody was used in a dilution of 1:3000, followed by anti-rabbit IgG, HRP conjugate diluted 1:5000.

    Techniques Used: SDS Page, Western Blot, Mutagenesis

    Related Articles

    Conjugation Assay:

    Article Title: Anti-c-Met antibodies recognising a temperature sensitive epitope, inhibit cell growth
    Article Snippet: .. HRP conjugation to seeMet antibodies was performed using EZ-link Plus Activated Peroxidase (Pierce/Thermo Scientific). .. HRP conjugated antibodies were used at 1 µg/mL on Western blotting.

    Incubation:

    Article Title: Herpes Simplex Virus Glycoprotein C Regulates Low pH Entry
    Article Snippet: .. After incubation with horseradish peroxidase-conjugated secondary antibodies, enhanced chemiluminescent substrate (Thermo Fisher Scientific) was added, and blots were exposed to X-ray film (Genesee Scientific). ..

    Binding Assay:

    Article Title: Intragastric administration of Lactobacillus casei expressing transmissible gastroentritis coronavirus spike glycoprotein induced specific antibody production
    Article Snippet: .. For the detection of specific antibody binding, horseradish peroxidase (HRP)-conjugated anti-swine IgG (ZYMED Laboratories Inc., San Francisco, USA) diluted at 1:10,000 were used. .. Visualization of the immunolabeled bands was then carried out using SuperSignal West Pico Chemiluminescent Substrate chemiluminescence reagent (Pierce Biotechnology, Rockford, USA), accordingly to manufacturer's instruction.

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  • 88
    Thermo Fisher anti syngap
    Model for the regulation of spine density by <t>CaMKIIα</t> activity. In the initial stage of spine formation, CaMKIIα has not yet been recruited to spines and Rap1 is not activated. In the subsequent step (arrow 1), glutamate receptors and CaMKIIα molecules begin to accumulate in spines, leading to the phosphorylation of <t>synGAP,</t> the dispersion of synGAP from postsynaptic sites, and the enhancement of local Rap1 activity. Increased Rap1 activity negatively regulates spine formation, possibly by destabilizing F-actin in spines (arrow 2). At a later stage, LTP-like spine-promoting mechanisms begin to operate (arrow 3).
    Anti Syngap, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti syngap/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti syngap - by Bioz Stars, 2020-10
    88/100 stars
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    90
    Thermo Fisher anti camkiiα
    CaMKII-dependent regulation of Rap1 activity. ( a ) Quantification of active Rap1 and active Ras immunoreactivity in the dendrites of <t>CaMKIIα</t> KI or wild-type neurons. Data are presented as the mean ± SEM, (neurons at 21–22
    Anti Camkiiα, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti camkiiα/product/Thermo Fisher
    Average 90 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    anti camkiiα - by Bioz Stars, 2020-10
    90/100 stars
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    Image Search Results


    Model for the regulation of spine density by CaMKIIα activity. In the initial stage of spine formation, CaMKIIα has not yet been recruited to spines and Rap1 is not activated. In the subsequent step (arrow 1), glutamate receptors and CaMKIIα molecules begin to accumulate in spines, leading to the phosphorylation of synGAP, the dispersion of synGAP from postsynaptic sites, and the enhancement of local Rap1 activity. Increased Rap1 activity negatively regulates spine formation, possibly by destabilizing F-actin in spines (arrow 2). At a later stage, LTP-like spine-promoting mechanisms begin to operate (arrow 3).

    Journal: Scientific Reports

    Article Title: Developmental stage-dependent regulation of spine formation by calcium-calmodulin-dependent protein kinase IIα and Rap1

    doi: 10.1038/s41598-017-13728-y

    Figure Lengend Snippet: Model for the regulation of spine density by CaMKIIα activity. In the initial stage of spine formation, CaMKIIα has not yet been recruited to spines and Rap1 is not activated. In the subsequent step (arrow 1), glutamate receptors and CaMKIIα molecules begin to accumulate in spines, leading to the phosphorylation of synGAP, the dispersion of synGAP from postsynaptic sites, and the enhancement of local Rap1 activity. Increased Rap1 activity negatively regulates spine formation, possibly by destabilizing F-actin in spines (arrow 2). At a later stage, LTP-like spine-promoting mechanisms begin to operate (arrow 3).

    Article Snippet: Reagents The following primary antibodies were used in this study: anti-CaMKIIα (Thermo Scientific), anti-phospho-CaMKIIα (Thr286) (Cell Signaling Technology), anti-synGAP (Thermo Scientific), anti-synGAP (phosphor S1123) (Abcam), anti-active Ras and anti-active Rap1, (NewEast Bioscience), anti-Rap1 (Santa Cruz or Abcam), anti-Ras (Abcam), anti-vGluT1, and anti-PSD95 (Thermo Scientific).

    Techniques: Activity Assay

    CaMKII-dependent regulation of Rap1 activity. ( a ) Quantification of active Rap1 and active Ras immunoreactivity in the dendrites of CaMKIIα KI or wild-type neurons. Data are presented as the mean ± SEM, (neurons at 21–22

    Journal: Scientific Reports

    Article Title: Developmental stage-dependent regulation of spine formation by calcium-calmodulin-dependent protein kinase IIα and Rap1

    doi: 10.1038/s41598-017-13728-y

    Figure Lengend Snippet: CaMKII-dependent regulation of Rap1 activity. ( a ) Quantification of active Rap1 and active Ras immunoreactivity in the dendrites of CaMKIIα KI or wild-type neurons. Data are presented as the mean ± SEM, (neurons at 21–22

    Article Snippet: The following primary antibodies were used in this study: anti-CaMKIIα (Thermo Scientific), anti-phospho-CaMKIIα (Thr286) (Cell Signaling Technology), anti-synGAP (Thermo Scientific), anti-synGAP (phosphor S1123) (Abcam), anti-active Ras and anti-active Rap1, (NewEast Bioscience), anti-Rap1 (Santa Cruz or Abcam), anti-Ras (Abcam), anti-vGluT1, and anti-PSD95 (Thermo Scientific).

    Techniques: Activity Assay

    Spine formation and elimination in CA1 pyramidal neurons in CaMKIIα KI and wild-type hippocampal slices. ( a and b ) Spines formed (arrows at Day2) and eliminated (arrows at Day1) in the oblique branches of CA1 pyramidal neurons in mature slices

    Journal: Scientific Reports

    Article Title: Developmental stage-dependent regulation of spine formation by calcium-calmodulin-dependent protein kinase IIα and Rap1

    doi: 10.1038/s41598-017-13728-y

    Figure Lengend Snippet: Spine formation and elimination in CA1 pyramidal neurons in CaMKIIα KI and wild-type hippocampal slices. ( a and b ) Spines formed (arrows at Day2) and eliminated (arrows at Day1) in the oblique branches of CA1 pyramidal neurons in mature slices

    Article Snippet: The following primary antibodies were used in this study: anti-CaMKIIα (Thermo Scientific), anti-phospho-CaMKIIα (Thr286) (Cell Signaling Technology), anti-synGAP (Thermo Scientific), anti-synGAP (phosphor S1123) (Abcam), anti-active Ras and anti-active Rap1, (NewEast Bioscience), anti-Rap1 (Santa Cruz or Abcam), anti-Ras (Abcam), anti-vGluT1, and anti-PSD95 (Thermo Scientific).

    Techniques:

    Model for the regulation of spine density by CaMKIIα activity. In the initial stage of spine formation, CaMKIIα has not yet been recruited to spines and Rap1 is not activated. In the subsequent step (arrow 1), glutamate receptors and CaMKIIα

    Journal: Scientific Reports

    Article Title: Developmental stage-dependent regulation of spine formation by calcium-calmodulin-dependent protein kinase IIα and Rap1

    doi: 10.1038/s41598-017-13728-y

    Figure Lengend Snippet: Model for the regulation of spine density by CaMKIIα activity. In the initial stage of spine formation, CaMKIIα has not yet been recruited to spines and Rap1 is not activated. In the subsequent step (arrow 1), glutamate receptors and CaMKIIα

    Article Snippet: The following primary antibodies were used in this study: anti-CaMKIIα (Thermo Scientific), anti-phospho-CaMKIIα (Thr286) (Cell Signaling Technology), anti-synGAP (Thermo Scientific), anti-synGAP (phosphor S1123) (Abcam), anti-active Ras and anti-active Rap1, (NewEast Bioscience), anti-Rap1 (Santa Cruz or Abcam), anti-Ras (Abcam), anti-vGluT1, and anti-PSD95 (Thermo Scientific).

    Techniques: Activity Assay

    Spine densities of wild-type and CaMKIIα KI hippocampal neurons maintained in slice cultures. ( a ) Representative images of oblique, apical, and basal dendrites at different developmental stages. Scale bars, 5 µm. ( b – d )

    Journal: Scientific Reports

    Article Title: Developmental stage-dependent regulation of spine formation by calcium-calmodulin-dependent protein kinase IIα and Rap1

    doi: 10.1038/s41598-017-13728-y

    Figure Lengend Snippet: Spine densities of wild-type and CaMKIIα KI hippocampal neurons maintained in slice cultures. ( a ) Representative images of oblique, apical, and basal dendrites at different developmental stages. Scale bars, 5 µm. ( b – d )

    Article Snippet: The following primary antibodies were used in this study: anti-CaMKIIα (Thermo Scientific), anti-phospho-CaMKIIα (Thr286) (Cell Signaling Technology), anti-synGAP (Thermo Scientific), anti-synGAP (phosphor S1123) (Abcam), anti-active Ras and anti-active Rap1, (NewEast Bioscience), anti-Rap1 (Santa Cruz or Abcam), anti-Ras (Abcam), anti-vGluT1, and anti-PSD95 (Thermo Scientific).

    Techniques:

    CaMKII-dependent regulation of Rap1 activity. ( a ) Quantification of active Rap1 and active Ras immunoreactivity in the dendrites of CaMKIIα KI or wild-type neurons. Data are presented as the mean ± SEM, (neurons at 21–22 DIV, Rap1; n = 42 and 27 cells from 4 independent cultures of wild-type and CaMKIIα KI hippocampi, Ras; n = 32 and 33 cells from 5 independent cultures of wild-type and CaMKIIα KI hippocampi), *p

    Journal: Scientific Reports

    Article Title: Developmental stage-dependent regulation of spine formation by calcium-calmodulin-dependent protein kinase IIα and Rap1

    doi: 10.1038/s41598-017-13728-y

    Figure Lengend Snippet: CaMKII-dependent regulation of Rap1 activity. ( a ) Quantification of active Rap1 and active Ras immunoreactivity in the dendrites of CaMKIIα KI or wild-type neurons. Data are presented as the mean ± SEM, (neurons at 21–22 DIV, Rap1; n = 42 and 27 cells from 4 independent cultures of wild-type and CaMKIIα KI hippocampi, Ras; n = 32 and 33 cells from 5 independent cultures of wild-type and CaMKIIα KI hippocampi), *p

    Article Snippet: Reagents The following primary antibodies were used in this study: anti-CaMKIIα (Thermo Scientific), anti-phospho-CaMKIIα (Thr286) (Cell Signaling Technology), anti-synGAP (Thermo Scientific), anti-synGAP (phosphor S1123) (Abcam), anti-active Ras and anti-active Rap1, (NewEast Bioscience), anti-Rap1 (Santa Cruz or Abcam), anti-Ras (Abcam), anti-vGluT1, and anti-PSD95 (Thermo Scientific).

    Techniques: Activity Assay

    Spine formation and elimination in CA1 pyramidal neurons in CaMKIIα KI and wild-type hippocampal slices. ( a and b ) Spines formed (arrows at Day2) and eliminated (arrows at Day1) in the oblique branches of CA1 pyramidal neurons in mature slices (19–23 DIV), as detected by time-lapse imaging of GFP-expressing cells over 24 h. The dendritic segments of wild-type ( a ) and KI ( b ) slices are presented. Scale bars, 5 µm. ( c ) Quantitative analysis of spine formation and elimination in wild-type and KI mice. Data are presented as the mean ± SEM, n = 5 cells from 2 independent cultures for wild-type mice, n = 5 cells from 3 independent cultures for KI mice, * p

    Journal: Scientific Reports

    Article Title: Developmental stage-dependent regulation of spine formation by calcium-calmodulin-dependent protein kinase IIα and Rap1

    doi: 10.1038/s41598-017-13728-y

    Figure Lengend Snippet: Spine formation and elimination in CA1 pyramidal neurons in CaMKIIα KI and wild-type hippocampal slices. ( a and b ) Spines formed (arrows at Day2) and eliminated (arrows at Day1) in the oblique branches of CA1 pyramidal neurons in mature slices (19–23 DIV), as detected by time-lapse imaging of GFP-expressing cells over 24 h. The dendritic segments of wild-type ( a ) and KI ( b ) slices are presented. Scale bars, 5 µm. ( c ) Quantitative analysis of spine formation and elimination in wild-type and KI mice. Data are presented as the mean ± SEM, n = 5 cells from 2 independent cultures for wild-type mice, n = 5 cells from 3 independent cultures for KI mice, * p

    Article Snippet: Reagents The following primary antibodies were used in this study: anti-CaMKIIα (Thermo Scientific), anti-phospho-CaMKIIα (Thr286) (Cell Signaling Technology), anti-synGAP (Thermo Scientific), anti-synGAP (phosphor S1123) (Abcam), anti-active Ras and anti-active Rap1, (NewEast Bioscience), anti-Rap1 (Santa Cruz or Abcam), anti-Ras (Abcam), anti-vGluT1, and anti-PSD95 (Thermo Scientific).

    Techniques: Imaging, Expressing, Mouse Assay

    Model for the regulation of spine density by CaMKIIα activity. In the initial stage of spine formation, CaMKIIα has not yet been recruited to spines and Rap1 is not activated. In the subsequent step (arrow 1), glutamate receptors and CaMKIIα molecules begin to accumulate in spines, leading to the phosphorylation of synGAP, the dispersion of synGAP from postsynaptic sites, and the enhancement of local Rap1 activity. Increased Rap1 activity negatively regulates spine formation, possibly by destabilizing F-actin in spines (arrow 2). At a later stage, LTP-like spine-promoting mechanisms begin to operate (arrow 3).

    Journal: Scientific Reports

    Article Title: Developmental stage-dependent regulation of spine formation by calcium-calmodulin-dependent protein kinase IIα and Rap1

    doi: 10.1038/s41598-017-13728-y

    Figure Lengend Snippet: Model for the regulation of spine density by CaMKIIα activity. In the initial stage of spine formation, CaMKIIα has not yet been recruited to spines and Rap1 is not activated. In the subsequent step (arrow 1), glutamate receptors and CaMKIIα molecules begin to accumulate in spines, leading to the phosphorylation of synGAP, the dispersion of synGAP from postsynaptic sites, and the enhancement of local Rap1 activity. Increased Rap1 activity negatively regulates spine formation, possibly by destabilizing F-actin in spines (arrow 2). At a later stage, LTP-like spine-promoting mechanisms begin to operate (arrow 3).

    Article Snippet: Reagents The following primary antibodies were used in this study: anti-CaMKIIα (Thermo Scientific), anti-phospho-CaMKIIα (Thr286) (Cell Signaling Technology), anti-synGAP (Thermo Scientific), anti-synGAP (phosphor S1123) (Abcam), anti-active Ras and anti-active Rap1, (NewEast Bioscience), anti-Rap1 (Santa Cruz or Abcam), anti-Ras (Abcam), anti-vGluT1, and anti-PSD95 (Thermo Scientific).

    Techniques: Activity Assay

    Spine densities of wild-type and CaMKIIα KI hippocampal neurons maintained in slice cultures. ( a ) Representative images of oblique, apical, and basal dendrites at different developmental stages. Scale bars, 5 µm. ( b – d ) Quantitative analysis of spine density in hippocampal pyramidal neurons maintained in slice cultures of wild-type and CaMKIIα KI mice at 9/10 DIV, 16/17 DIV, 23/24 DIV and 30/31 DIV. Spine density was measured from images of oblique ( b ), apical ( c ), and basal ( d ) branches of dendrites, collected from 7–22 experiments. Data are presented as the mean ± SEM, n = 7–22 cells, * p

    Journal: Scientific Reports

    Article Title: Developmental stage-dependent regulation of spine formation by calcium-calmodulin-dependent protein kinase IIα and Rap1

    doi: 10.1038/s41598-017-13728-y

    Figure Lengend Snippet: Spine densities of wild-type and CaMKIIα KI hippocampal neurons maintained in slice cultures. ( a ) Representative images of oblique, apical, and basal dendrites at different developmental stages. Scale bars, 5 µm. ( b – d ) Quantitative analysis of spine density in hippocampal pyramidal neurons maintained in slice cultures of wild-type and CaMKIIα KI mice at 9/10 DIV, 16/17 DIV, 23/24 DIV and 30/31 DIV. Spine density was measured from images of oblique ( b ), apical ( c ), and basal ( d ) branches of dendrites, collected from 7–22 experiments. Data are presented as the mean ± SEM, n = 7–22 cells, * p

    Article Snippet: Reagents The following primary antibodies were used in this study: anti-CaMKIIα (Thermo Scientific), anti-phospho-CaMKIIα (Thr286) (Cell Signaling Technology), anti-synGAP (Thermo Scientific), anti-synGAP (phosphor S1123) (Abcam), anti-active Ras and anti-active Rap1, (NewEast Bioscience), anti-Rap1 (Santa Cruz or Abcam), anti-Ras (Abcam), anti-vGluT1, and anti-PSD95 (Thermo Scientific).

    Techniques: Mouse Assay