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Promega hrp conjugate
Hrp Conjugate, supplied by Promega, used in various techniques. Bioz Stars score: 98/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hrp conjugate/product/Promega
Average 98 stars, based on 16 article reviews
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hrp conjugate - by Bioz Stars, 2020-02
98/100 stars

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Related Articles

Transfection:

Article Title: Knockout Studies Reveal an Important Role of Plasmodium Lipoic Acid Protein Ligase A1 for Asexual Blood Stage Parasite Survival
Article Snippet: Western blot analyses To detect whether P. falciparum transfected with pHrBI-PbLplA1 alone or P. falciparum co-transfected with pHH1-LplA1 -KO and pHrBI-PbLplA1 expressed P. berghei LpA1 protein in addition to the endogenous P. falciparum LplA1 protein, parasite extracts of wild-type and mutant parasites were subjected to western blot analyses. .. The blots were incubated with a rabbit anti-LplA1 antibody (generated against recombinant protein P. falciparum LplA1 by Eurogentec, Belgium) at a dilution of 1∶5000 and the secondary anti-rabbit IgG (H+L), HRP conjugate (Promega) at a dilution of 1∶10,000 before being developed using the Immobilon™ Western Chemiluminescent HRP Substrate (Millipore).

Mutagenesis:

Article Title: Knockout Studies Reveal an Important Role of Plasmodium Lipoic Acid Protein Ligase A1 for Asexual Blood Stage Parasite Survival
Article Snippet: Western blot analyses To detect whether P. falciparum transfected with pHrBI-PbLplA1 alone or P. falciparum co-transfected with pHH1-LplA1 -KO and pHrBI-PbLplA1 expressed P. berghei LpA1 protein in addition to the endogenous P. falciparum LplA1 protein, parasite extracts of wild-type and mutant parasites were subjected to western blot analyses. .. The blots were incubated with a rabbit anti-LplA1 antibody (generated against recombinant protein P. falciparum LplA1 by Eurogentec, Belgium) at a dilution of 1∶5000 and the secondary anti-rabbit IgG (H+L), HRP conjugate (Promega) at a dilution of 1∶10,000 before being developed using the Immobilon™ Western Chemiluminescent HRP Substrate (Millipore).

Incubation:

Article Title: Enhanced Delivery of Oncolytic Adenovirus by Neural Stem Cells for Treatment of Metastatic Ovarian Cancer
Article Snippet: .. Membranes were then incubated in a 1:10,000 solution of anti-human Ig (H+L), HRP conjugate (Promega) for 90 min at room temperature. .. After washing, membranes were developed with 3,3’, 5,5’-tetramethylbenzidine (TMB) stabilized substrate for horseradish peroxidase (Promega) and imaged.

Article Title: Knockout Studies Reveal an Important Role of Plasmodium Lipoic Acid Protein Ligase A1 for Asexual Blood Stage Parasite Survival
Article Snippet: .. The blots were incubated with a rabbit anti-LplA1 antibody (generated against recombinant protein P. falciparum LplA1 by Eurogentec, Belgium) at a dilution of 1∶5000 and the secondary anti-rabbit IgG (H+L), HRP conjugate (Promega) at a dilution of 1∶10,000 before being developed using the Immobilon™ Western Chemiluminescent HRP Substrate (Millipore). .. PCR analyses of P. berghei transfectants The parasite populations that were isolated from infected mice after transfection of the three P. berghei constructs were analysed by PCRs using diagnostic primer combinations that allowed determining whether (i) the gene locus had been targeted by the plasmid, (ii) the endogenous gene was still present and (iii) the plasmid had recombined and was present as an episome.

Article Title: Apicoplast Lipoic Acid Protein Ligase B Is Not Essential for Plasmodium falciparum
Article Snippet: .. The blot was incubated with a rabbit anti-LA antibody (Calbiochem) at a dilution of 1:500 and the secondary anti-rabbit IgG (H+L), HRP conjugate (Promega) at a dilution of 1:10,000 before being developed using the ImmobilonTM Western Chemiluminescent HRP Substrate (Millipore). .. Similarly, blots of wild-type parasite extracts were also probed with antibodies against BCDH-E2 (raised in rabbit), KGDH-E2 (raised in rat) and H-protein (raised in rabbit) of P. falciparum (all generated by Eurogentec, Belgium) at dilutions of 1:5,000, 1:100 and 1:2,000, respectively.

Microscopy:

Article Title: Two Closely Related Human Members of Chitinase-like Family, CHI3L1 and CHI3L2, Activate ERK1/2 in 293 and U373 Cells but Have the Different Influence on Cell Proliferation
Article Snippet: .. Antibodies were obtained from multiple sources and used at the specified dilutions for immunofluorescence microscopy: p44/42 MAP (Erk1/2) (L34F12) Mouse mAb (Cell Signaling Technology, Danvers, MA) 1:100 (anti-phospho-ERK); ERK1 (K-23) Rabbit polyclonal IgG (Santa Cruz Biotechnology, Santa Cruz, CA) 1:100 (pan-anti-ERK); Fluorescein Anti-Mouse IgG (H+L) as well as Texas Red® Anti-Rabbit IgG (Vector Laboratories Inc., Burlingame, CA) 1:400; and for immunoblots: pERK (E-4) Mouse monoclonal IgG (Santa Cruz) 1:2000 (anti-phospho-ERK); ERK1 (K-23) Rabbit polyclonal IgG 1:3000 (pan-anti-ERK); Anti-Mouse IgG (H+L), HRP Conjugate and Anti-Rabbit IgG (H+L), HRP Conjugate (Promega) 1:20000 (HRP-anti-mouse IgG), (HRP-anti-rabbit IgG). .. The reagents for enhanced chemiluminescence (ECL) from Sigma-Aldrich Co. and Fluka (Buchs, Switzerland) were used for the visualization of immunoreactive bands on Western blots.

Purification:

Article Title: HPLC Analysis and Biochemical Characterization of LOX from Eschscholtzia californica Cham.
Article Snippet: Commercially available Anti-Rabbit IgG (H+L), HRP Conjugate (Promega, Madison, WI, USA) was used as a secondary antibody. .. The molecular mass of the purified enzyme was assessed by comparing the mobility of LOX protein with the mobility of molecular markers in 10% SDS-polyacrylamide gel.

Article Title: Two Closely Related Human Members of Chitinase-like Family, CHI3L1 and CHI3L2, Activate ERK1/2 in 293 and U373 Cells but Have the Different Influence on Cell Proliferation
Article Snippet: Purified CHI3L1 and CHI3L2 were stored frozen at -20°C in aliquots to avoid repeated freeze-thawing and denaturation. .. Antibodies were obtained from multiple sources and used at the specified dilutions for immunofluorescence microscopy: p44/42 MAP (Erk1/2) (L34F12) Mouse mAb (Cell Signaling Technology, Danvers, MA) 1:100 (anti-phospho-ERK); ERK1 (K-23) Rabbit polyclonal IgG (Santa Cruz Biotechnology, Santa Cruz, CA) 1:100 (pan-anti-ERK); Fluorescein Anti-Mouse IgG (H+L) as well as Texas Red® Anti-Rabbit IgG (Vector Laboratories Inc., Burlingame, CA) 1:400; and for immunoblots: pERK (E-4) Mouse monoclonal IgG (Santa Cruz) 1:2000 (anti-phospho-ERK); ERK1 (K-23) Rabbit polyclonal IgG 1:3000 (pan-anti-ERK); Anti-Mouse IgG (H+L), HRP Conjugate and Anti-Rabbit IgG (H+L), HRP Conjugate (Promega) 1:20000 (HRP-anti-mouse IgG), (HRP-anti-rabbit IgG).

Electrophoresis:

Article Title: Enhanced Delivery of Oncolytic Adenovirus by Neural Stem Cells for Treatment of Metastatic Ovarian Cancer
Article Snippet: Following electrophoresis, proteins were transferred by semi-dry electroblotting onto a nitrocellulose membrane (Bio-Rad), which was blocked with 5% non-fat dried milk/0.1% Tween in PBS (PBST) for 90 min at room temperature. .. Membranes were then incubated in a 1:10,000 solution of anti-human Ig (H+L), HRP conjugate (Promega) for 90 min at room temperature.

Article Title: HPLC Analysis and Biochemical Characterization of LOX from Eschscholtzia californica Cham.
Article Snippet: Paragraph title: 3.9. Electrophoresis and Western Blotting ... Commercially available Anti-Rabbit IgG (H+L), HRP Conjugate (Promega, Madison, WI, USA) was used as a secondary antibody.

Generated:

Article Title: Knockout Studies Reveal an Important Role of Plasmodium Lipoic Acid Protein Ligase A1 for Asexual Blood Stage Parasite Survival
Article Snippet: .. The blots were incubated with a rabbit anti-LplA1 antibody (generated against recombinant protein P. falciparum LplA1 by Eurogentec, Belgium) at a dilution of 1∶5000 and the secondary anti-rabbit IgG (H+L), HRP conjugate (Promega) at a dilution of 1∶10,000 before being developed using the Immobilon™ Western Chemiluminescent HRP Substrate (Millipore). .. PCR analyses of P. berghei transfectants The parasite populations that were isolated from infected mice after transfection of the three P. berghei constructs were analysed by PCRs using diagnostic primer combinations that allowed determining whether (i) the gene locus had been targeted by the plasmid, (ii) the endogenous gene was still present and (iii) the plasmid had recombined and was present as an episome.

Article Title: Apicoplast Lipoic Acid Protein Ligase B Is Not Essential for Plasmodium falciparum
Article Snippet: The blot was incubated with a rabbit anti-LA antibody (Calbiochem) at a dilution of 1:500 and the secondary anti-rabbit IgG (H+L), HRP conjugate (Promega) at a dilution of 1:10,000 before being developed using the ImmobilonTM Western Chemiluminescent HRP Substrate (Millipore). .. Similarly, blots of wild-type parasite extracts were also probed with antibodies against BCDH-E2 (raised in rabbit), KGDH-E2 (raised in rat) and H-protein (raised in rabbit) of P. falciparum (all generated by Eurogentec, Belgium) at dilutions of 1:5,000, 1:100 and 1:2,000, respectively.

Activity Assay:

Article Title: Apicoplast Lipoic Acid Protein Ligase B Is Not Essential for Plasmodium falciparum
Article Snippet: Anti-LA rabbit polyclonal antibody was supplied by Calbiochem and the anti-rabbit IgG (H+L), HRP conjugate was from Promega. .. [α-32 P]-ATP (Adenosine 5′-triphosphate [α-32 P], EasyTides, specific activity: 3,000 Ci/mmol) was purchased from Perkin-Elmer.

Western Blot:

Article Title: Enhanced Delivery of Oncolytic Adenovirus by Neural Stem Cells for Treatment of Metastatic Ovarian Cancer
Article Snippet: Neutralizing antibodies against CRAd-S-pk7 were recognized in ascites samples by western blotting as previously described. .. Membranes were then incubated in a 1:10,000 solution of anti-human Ig (H+L), HRP conjugate (Promega) for 90 min at room temperature.

Article Title: Apicoplast Lipoic Acid Protein Ligase B Is Not Essential for Plasmodium falciparum
Article Snippet: The ImmobilonTM Western Chemiluminescent HRP Substrate was obtained from Millipore, UK. .. Anti-LA rabbit polyclonal antibody was supplied by Calbiochem and the anti-rabbit IgG (H+L), HRP conjugate was from Promega.

Article Title: HPLC Analysis and Biochemical Characterization of LOX from Eschscholtzia californica Cham.
Article Snippet: Paragraph title: 3.9. Electrophoresis and Western Blotting ... Commercially available Anti-Rabbit IgG (H+L), HRP Conjugate (Promega, Madison, WI, USA) was used as a secondary antibody.

Article Title: Monitoring and characterizing soluble and membrane-bound ectonucleotidases CD73 and CD39
Article Snippet: For Western Blot, TBS buffer (50mM Tris Buffer, pH 7.5, 150mM NaCl), TBST (50mM Tris Buffer, pH 7.5, 150mM NaCl, and 0.1% Tween 20), and antibody dilution buffer (TBST with 5% BSA) were used. .. Secondary antibodies: Donkey Anti-Rabbit IgG (H+L), and HRP Conjugate, Anti-Mouse IgG (H+L), HRP Conjugate from Promega Corporation (Madison, WI).

Article Title: Knockout Studies Reveal an Important Role of Plasmodium Lipoic Acid Protein Ligase A1 for Asexual Blood Stage Parasite Survival
Article Snippet: .. The blots were incubated with a rabbit anti-LplA1 antibody (generated against recombinant protein P. falciparum LplA1 by Eurogentec, Belgium) at a dilution of 1∶5000 and the secondary anti-rabbit IgG (H+L), HRP conjugate (Promega) at a dilution of 1∶10,000 before being developed using the Immobilon™ Western Chemiluminescent HRP Substrate (Millipore). .. PCR analyses of P. berghei transfectants The parasite populations that were isolated from infected mice after transfection of the three P. berghei constructs were analysed by PCRs using diagnostic primer combinations that allowed determining whether (i) the gene locus had been targeted by the plasmid, (ii) the endogenous gene was still present and (iii) the plasmid had recombined and was present as an episome.

Article Title: Apicoplast Lipoic Acid Protein Ligase B Is Not Essential for Plasmodium falciparum
Article Snippet: .. The blot was incubated with a rabbit anti-LA antibody (Calbiochem) at a dilution of 1:500 and the secondary anti-rabbit IgG (H+L), HRP conjugate (Promega) at a dilution of 1:10,000 before being developed using the ImmobilonTM Western Chemiluminescent HRP Substrate (Millipore). .. Similarly, blots of wild-type parasite extracts were also probed with antibodies against BCDH-E2 (raised in rabbit), KGDH-E2 (raised in rat) and H-protein (raised in rabbit) of P. falciparum (all generated by Eurogentec, Belgium) at dilutions of 1:5,000, 1:100 and 1:2,000, respectively.

Article Title: Two Closely Related Human Members of Chitinase-like Family, CHI3L1 and CHI3L2, Activate ERK1/2 in 293 and U373 Cells but Have the Different Influence on Cell Proliferation
Article Snippet: .. Antibodies were obtained from multiple sources and used at the specified dilutions for immunofluorescence microscopy: p44/42 MAP (Erk1/2) (L34F12) Mouse mAb (Cell Signaling Technology, Danvers, MA) 1:100 (anti-phospho-ERK); ERK1 (K-23) Rabbit polyclonal IgG (Santa Cruz Biotechnology, Santa Cruz, CA) 1:100 (pan-anti-ERK); Fluorescein Anti-Mouse IgG (H+L) as well as Texas Red® Anti-Rabbit IgG (Vector Laboratories Inc., Burlingame, CA) 1:400; and for immunoblots: pERK (E-4) Mouse monoclonal IgG (Santa Cruz) 1:2000 (anti-phospho-ERK); ERK1 (K-23) Rabbit polyclonal IgG 1:3000 (pan-anti-ERK); Anti-Mouse IgG (H+L), HRP Conjugate and Anti-Rabbit IgG (H+L), HRP Conjugate (Promega) 1:20000 (HRP-anti-mouse IgG), (HRP-anti-rabbit IgG). .. The reagents for enhanced chemiluminescence (ECL) from Sigma-Aldrich Co. and Fluka (Buchs, Switzerland) were used for the visualization of immunoreactive bands on Western blots.

Staining:

Article Title: HPLC Analysis and Biochemical Characterization of LOX from Eschscholtzia californica Cham.
Article Snippet: The gel were stained with PageBlueTM Protein Staining Solution (Thermo Scientific, Waltham, MA, USA) with Coomassie Briliant Blue G 250 and destained in 25% ethanol, 10% acetic acid, and 65% redistilled water (v /v /v ). .. Commercially available Anti-Rabbit IgG (H+L), HRP Conjugate (Promega, Madison, WI, USA) was used as a secondary antibody.

Article Title: Two Closely Related Human Members of Chitinase-like Family, CHI3L1 and CHI3L2, Activate ERK1/2 in 293 and U373 Cells but Have the Different Influence on Cell Proliferation
Article Snippet: Protein products were analyzed by 12 % SDS-PAGE and Coomassie Brilliant Blue R250 staining. .. Antibodies were obtained from multiple sources and used at the specified dilutions for immunofluorescence microscopy: p44/42 MAP (Erk1/2) (L34F12) Mouse mAb (Cell Signaling Technology, Danvers, MA) 1:100 (anti-phospho-ERK); ERK1 (K-23) Rabbit polyclonal IgG (Santa Cruz Biotechnology, Santa Cruz, CA) 1:100 (pan-anti-ERK); Fluorescein Anti-Mouse IgG (H+L) as well as Texas Red® Anti-Rabbit IgG (Vector Laboratories Inc., Burlingame, CA) 1:400; and for immunoblots: pERK (E-4) Mouse monoclonal IgG (Santa Cruz) 1:2000 (anti-phospho-ERK); ERK1 (K-23) Rabbit polyclonal IgG 1:3000 (pan-anti-ERK); Anti-Mouse IgG (H+L), HRP Conjugate and Anti-Rabbit IgG (H+L), HRP Conjugate (Promega) 1:20000 (HRP-anti-mouse IgG), (HRP-anti-rabbit IgG).

Recombinant:

Article Title: Knockout Studies Reveal an Important Role of Plasmodium Lipoic Acid Protein Ligase A1 for Asexual Blood Stage Parasite Survival
Article Snippet: .. The blots were incubated with a rabbit anti-LplA1 antibody (generated against recombinant protein P. falciparum LplA1 by Eurogentec, Belgium) at a dilution of 1∶5000 and the secondary anti-rabbit IgG (H+L), HRP conjugate (Promega) at a dilution of 1∶10,000 before being developed using the Immobilon™ Western Chemiluminescent HRP Substrate (Millipore). .. PCR analyses of P. berghei transfectants The parasite populations that were isolated from infected mice after transfection of the three P. berghei constructs were analysed by PCRs using diagnostic primer combinations that allowed determining whether (i) the gene locus had been targeted by the plasmid, (ii) the endogenous gene was still present and (iii) the plasmid had recombined and was present as an episome.

Article Title: Two Closely Related Human Members of Chitinase-like Family, CHI3L1 and CHI3L2, Activate ERK1/2 in 293 and U373 Cells but Have the Different Influence on Cell Proliferation
Article Snippet: Synthesis of recombinant CHI3L2 was performed according to our previous description . .. Antibodies were obtained from multiple sources and used at the specified dilutions for immunofluorescence microscopy: p44/42 MAP (Erk1/2) (L34F12) Mouse mAb (Cell Signaling Technology, Danvers, MA) 1:100 (anti-phospho-ERK); ERK1 (K-23) Rabbit polyclonal IgG (Santa Cruz Biotechnology, Santa Cruz, CA) 1:100 (pan-anti-ERK); Fluorescein Anti-Mouse IgG (H+L) as well as Texas Red® Anti-Rabbit IgG (Vector Laboratories Inc., Burlingame, CA) 1:400; and for immunoblots: pERK (E-4) Mouse monoclonal IgG (Santa Cruz) 1:2000 (anti-phospho-ERK); ERK1 (K-23) Rabbit polyclonal IgG 1:3000 (pan-anti-ERK); Anti-Mouse IgG (H+L), HRP Conjugate and Anti-Rabbit IgG (H+L), HRP Conjugate (Promega) 1:20000 (HRP-anti-mouse IgG), (HRP-anti-rabbit IgG).

Immunofluorescence:

Article Title: Two Closely Related Human Members of Chitinase-like Family, CHI3L1 and CHI3L2, Activate ERK1/2 in 293 and U373 Cells but Have the Different Influence on Cell Proliferation
Article Snippet: .. Antibodies were obtained from multiple sources and used at the specified dilutions for immunofluorescence microscopy: p44/42 MAP (Erk1/2) (L34F12) Mouse mAb (Cell Signaling Technology, Danvers, MA) 1:100 (anti-phospho-ERK); ERK1 (K-23) Rabbit polyclonal IgG (Santa Cruz Biotechnology, Santa Cruz, CA) 1:100 (pan-anti-ERK); Fluorescein Anti-Mouse IgG (H+L) as well as Texas Red® Anti-Rabbit IgG (Vector Laboratories Inc., Burlingame, CA) 1:400; and for immunoblots: pERK (E-4) Mouse monoclonal IgG (Santa Cruz) 1:2000 (anti-phospho-ERK); ERK1 (K-23) Rabbit polyclonal IgG 1:3000 (pan-anti-ERK); Anti-Mouse IgG (H+L), HRP Conjugate and Anti-Rabbit IgG (H+L), HRP Conjugate (Promega) 1:20000 (HRP-anti-mouse IgG), (HRP-anti-rabbit IgG). .. The reagents for enhanced chemiluminescence (ECL) from Sigma-Aldrich Co. and Fluka (Buchs, Switzerland) were used for the visualization of immunoreactive bands on Western blots.

SDS Page:

Article Title: Enhanced Delivery of Oncolytic Adenovirus by Neural Stem Cells for Treatment of Metastatic Ovarian Cancer
Article Snippet: In brief, CRAd-S-pk7 was diluted in PBS to concentrations of 125, 250, 500, and 1,000 ng protein/mL, then subjected to SDS-PAGE (10% NuPAGE Bis-Tris Gel; Thermo Fisher). .. Membranes were then incubated in a 1:10,000 solution of anti-human Ig (H+L), HRP conjugate (Promega) for 90 min at room temperature.

Article Title: HPLC Analysis and Biochemical Characterization of LOX from Eschscholtzia californica Cham.
Article Snippet: Proteins separated by SDS-PAGE were transferred to a nitrocellulose membrane of 0.45 μm (Advantec® MFS, Suite A Dublin, CA, USA) using TRANS-BLOT SD (BIO-RAD, Hercules, CA, USA) according to Towbin et al. [ ]. .. Commercially available Anti-Rabbit IgG (H+L), HRP Conjugate (Promega, Madison, WI, USA) was used as a secondary antibody.

Article Title: Monitoring and characterizing soluble and membrane-bound ectonucleotidases CD73 and CD39
Article Snippet: Reagents for SDS-PAGE include Tris-Glycine Running Buffer: 25mM Trizma base, 192mM glycine free base, with 0.1% SDS. .. Secondary antibodies: Donkey Anti-Rabbit IgG (H+L), and HRP Conjugate, Anti-Mouse IgG (H+L), HRP Conjugate from Promega Corporation (Madison, WI).

Article Title: Knockout Studies Reveal an Important Role of Plasmodium Lipoic Acid Protein Ligase A1 for Asexual Blood Stage Parasite Survival
Article Snippet: Briefly, 15 µg of P. falciparum lysates or the protein extract obtained from 1×106 or 0.2×107 P. berghei were separated on a 4–12% SDS-PAGE (Invitrogen) and then blotted onto nitrocellulose (Schleicher and Schüll), using standard techniques . .. The blots were incubated with a rabbit anti-LplA1 antibody (generated against recombinant protein P. falciparum LplA1 by Eurogentec, Belgium) at a dilution of 1∶5000 and the secondary anti-rabbit IgG (H+L), HRP conjugate (Promega) at a dilution of 1∶10,000 before being developed using the Immobilon™ Western Chemiluminescent HRP Substrate (Millipore).

Article Title: Apicoplast Lipoic Acid Protein Ligase B Is Not Essential for Plasmodium falciparum
Article Snippet: Briefly, 15 μg of each sample was separated on a 4%-12% SDS-PAGE (Invitrogen) and then blotted onto nitrocellulose (Schleicher and Schüll), using standard techniques [ ]. .. The blot was incubated with a rabbit anti-LA antibody (Calbiochem) at a dilution of 1:500 and the secondary anti-rabbit IgG (H+L), HRP conjugate (Promega) at a dilution of 1:10,000 before being developed using the ImmobilonTM Western Chemiluminescent HRP Substrate (Millipore).

Article Title: Two Closely Related Human Members of Chitinase-like Family, CHI3L1 and CHI3L2, Activate ERK1/2 in 293 and U373 Cells but Have the Different Influence on Cell Proliferation
Article Snippet: Protein products were analyzed by 12 % SDS-PAGE and Coomassie Brilliant Blue R250 staining. .. Antibodies were obtained from multiple sources and used at the specified dilutions for immunofluorescence microscopy: p44/42 MAP (Erk1/2) (L34F12) Mouse mAb (Cell Signaling Technology, Danvers, MA) 1:100 (anti-phospho-ERK); ERK1 (K-23) Rabbit polyclonal IgG (Santa Cruz Biotechnology, Santa Cruz, CA) 1:100 (pan-anti-ERK); Fluorescein Anti-Mouse IgG (H+L) as well as Texas Red® Anti-Rabbit IgG (Vector Laboratories Inc., Burlingame, CA) 1:400; and for immunoblots: pERK (E-4) Mouse monoclonal IgG (Santa Cruz) 1:2000 (anti-phospho-ERK); ERK1 (K-23) Rabbit polyclonal IgG 1:3000 (pan-anti-ERK); Anti-Mouse IgG (H+L), HRP Conjugate and Anti-Rabbit IgG (H+L), HRP Conjugate (Promega) 1:20000 (HRP-anti-mouse IgG), (HRP-anti-rabbit IgG).

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    Promega anti hrp
    The large Dystrophin isoforms are localized postsynaptically. Third-instar larval body walls were stained with anti-DysCO 2 H, which recognizes all Dystrophin isoforms ( A–C ), <t>anti-Dyslarge,</t> which recognizes only the large isoforms ( D , E ), double labeled with <t>anti-HRP</t> and anti-DysCO 2 H ( F ), or double labeled with anti-actin and anti-DysCO 2 H ( G ). A , Dystrophin protein is expressed at the wild-type third-instar larval NMJ at synaptic and extrasynaptic sites. B , Dystrophin protein is severely reduced in the dys GE20705 mutant. C , The DLP2 isoform protein accumulates highly at the NMJ after overexpression in the muscle (G14-Gal4/+; GS12472/+). D , Wild-type Dystrophin protein is recognized at the NMJ by the large-isoform-specific antibody anti-Dyslarge. E , Overexpressed DLP2 (G14-Gal4/+; GS12472/+) can also be visualized using anti-Dyslarge antisera. F , Double labeling of a wild-type larval body wall with anti-HRP, staining presynaptic boutons (green), and anti-DysCO 2 H (red) reveals that the Dystrophin protein is postsynaptically localized at the NMJ. G , Double labeling of a wild-type larval body wall with anti-actin (green) and anti-DysCO 2 H (red) reveals that the Dystrophin colocalizes with actin at the NMJ extrasynaptic sites of expression. H , RNA in situ hybridization of wild-type larval neuropil (filled arrow), brain (arrowhead), and associated eye-antennal discs (open arrow) with an exon 4 antisense probe that labels all large dystrophin isoform mRNAs reveals no apparent expression of large dystrophin isoform mRNAs in the neuropil or brain. I , Western blot analysis of embryo extracts prepared from wild-type, dys GE20705 , Elav-Gal4/GS12472 (overproducing DLP2), and Elav-Gal4/UAS-Dp186 (overproducing Dp186) using the indicated antibodies. The large Dystrophin isoforms (anti-Dyslarge panel) are absent from the mutant dys GE20705 and overexpressed in the Elav-Gal4/GS12472 embryos. Dp186 is expressed at decreased levels in dys GE20705 and is overexpressed in Elav-Gal4/UAS-Dp186 (anti-Dp186 panel). The pan-Dystrophin antibody (anti-dysCO 2 H panel) confirms the overexpression of both the long isoforms and Dp186 in the overproducing embryos. The arrows indicate the large Dystrophin isoforms, and the short arrows indicate the Dp186 isoform. All lanes were loaded equally, as confirmed by the anti-ribosomal subunit PS3 antibody (anti-PS3 panel), except the last lane of the Dp186 blot, in which 5% of the protein was loaded to permit comparison of the extracts in a single exposure.
    Anti Hrp, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti hrp/product/Promega
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti hrp - by Bioz Stars, 2020-02
    86/100 stars
      Buy from Supplier

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    The large Dystrophin isoforms are localized postsynaptically. Third-instar larval body walls were stained with anti-DysCO 2 H, which recognizes all Dystrophin isoforms ( A–C ), anti-Dyslarge, which recognizes only the large isoforms ( D , E ), double labeled with anti-HRP and anti-DysCO 2 H ( F ), or double labeled with anti-actin and anti-DysCO 2 H ( G ). A , Dystrophin protein is expressed at the wild-type third-instar larval NMJ at synaptic and extrasynaptic sites. B , Dystrophin protein is severely reduced in the dys GE20705 mutant. C , The DLP2 isoform protein accumulates highly at the NMJ after overexpression in the muscle (G14-Gal4/+; GS12472/+). D , Wild-type Dystrophin protein is recognized at the NMJ by the large-isoform-specific antibody anti-Dyslarge. E , Overexpressed DLP2 (G14-Gal4/+; GS12472/+) can also be visualized using anti-Dyslarge antisera. F , Double labeling of a wild-type larval body wall with anti-HRP, staining presynaptic boutons (green), and anti-DysCO 2 H (red) reveals that the Dystrophin protein is postsynaptically localized at the NMJ. G , Double labeling of a wild-type larval body wall with anti-actin (green) and anti-DysCO 2 H (red) reveals that the Dystrophin colocalizes with actin at the NMJ extrasynaptic sites of expression. H , RNA in situ hybridization of wild-type larval neuropil (filled arrow), brain (arrowhead), and associated eye-antennal discs (open arrow) with an exon 4 antisense probe that labels all large dystrophin isoform mRNAs reveals no apparent expression of large dystrophin isoform mRNAs in the neuropil or brain. I , Western blot analysis of embryo extracts prepared from wild-type, dys GE20705 , Elav-Gal4/GS12472 (overproducing DLP2), and Elav-Gal4/UAS-Dp186 (overproducing Dp186) using the indicated antibodies. The large Dystrophin isoforms (anti-Dyslarge panel) are absent from the mutant dys GE20705 and overexpressed in the Elav-Gal4/GS12472 embryos. Dp186 is expressed at decreased levels in dys GE20705 and is overexpressed in Elav-Gal4/UAS-Dp186 (anti-Dp186 panel). The pan-Dystrophin antibody (anti-dysCO 2 H panel) confirms the overexpression of both the long isoforms and Dp186 in the overproducing embryos. The arrows indicate the large Dystrophin isoforms, and the short arrows indicate the Dp186 isoform. All lanes were loaded equally, as confirmed by the anti-ribosomal subunit PS3 antibody (anti-PS3 panel), except the last lane of the Dp186 blot, in which 5% of the protein was loaded to permit comparison of the extracts in a single exposure.

    Journal: The Journal of Neuroscience

    Article Title: Dystrophin Is Required for Appropriate Retrograde Control of Neurotransmitter Release at the Drosophila Neuromuscular Junction

    doi: 10.1523/JNEUROSCI.4069-05.2006

    Figure Lengend Snippet: The large Dystrophin isoforms are localized postsynaptically. Third-instar larval body walls were stained with anti-DysCO 2 H, which recognizes all Dystrophin isoforms ( A–C ), anti-Dyslarge, which recognizes only the large isoforms ( D , E ), double labeled with anti-HRP and anti-DysCO 2 H ( F ), or double labeled with anti-actin and anti-DysCO 2 H ( G ). A , Dystrophin protein is expressed at the wild-type third-instar larval NMJ at synaptic and extrasynaptic sites. B , Dystrophin protein is severely reduced in the dys GE20705 mutant. C , The DLP2 isoform protein accumulates highly at the NMJ after overexpression in the muscle (G14-Gal4/+; GS12472/+). D , Wild-type Dystrophin protein is recognized at the NMJ by the large-isoform-specific antibody anti-Dyslarge. E , Overexpressed DLP2 (G14-Gal4/+; GS12472/+) can also be visualized using anti-Dyslarge antisera. F , Double labeling of a wild-type larval body wall with anti-HRP, staining presynaptic boutons (green), and anti-DysCO 2 H (red) reveals that the Dystrophin protein is postsynaptically localized at the NMJ. G , Double labeling of a wild-type larval body wall with anti-actin (green) and anti-DysCO 2 H (red) reveals that the Dystrophin colocalizes with actin at the NMJ extrasynaptic sites of expression. H , RNA in situ hybridization of wild-type larval neuropil (filled arrow), brain (arrowhead), and associated eye-antennal discs (open arrow) with an exon 4 antisense probe that labels all large dystrophin isoform mRNAs reveals no apparent expression of large dystrophin isoform mRNAs in the neuropil or brain. I , Western blot analysis of embryo extracts prepared from wild-type, dys GE20705 , Elav-Gal4/GS12472 (overproducing DLP2), and Elav-Gal4/UAS-Dp186 (overproducing Dp186) using the indicated antibodies. The large Dystrophin isoforms (anti-Dyslarge panel) are absent from the mutant dys GE20705 and overexpressed in the Elav-Gal4/GS12472 embryos. Dp186 is expressed at decreased levels in dys GE20705 and is overexpressed in Elav-Gal4/UAS-Dp186 (anti-Dp186 panel). The pan-Dystrophin antibody (anti-dysCO 2 H panel) confirms the overexpression of both the long isoforms and Dp186 in the overproducing embryos. The arrows indicate the large Dystrophin isoforms, and the short arrows indicate the Dp186 isoform. All lanes were loaded equally, as confirmed by the anti-ribosomal subunit PS3 antibody (anti-PS3 panel), except the last lane of the Dp186 blot, in which 5% of the protein was loaded to permit comparison of the extracts in a single exposure.

    Article Snippet: Anti-dysCO2 H (1:3000), anti-Dyslarge (1:3000), anti-HRP (1:500) (Promega, Madison, WI), anti-DGluRIIA (1:500) (Developmental Studies Hybridoma Bank, University of Iowa, Iowa City, IA) , anti-DGluRIIB (1:2500) , anti-Discslarge (Developmental Studies Hybridoma Bank) ( ) (1:500), anti-actin (1:5000) (MP Biomedicals, Aurora, OH), anti-Phospho-Mad ( ) (1:1000), Alexa Fluor-conjugated secondary antibodies (Invitrogen, Breda, The Netherlands), and an anti-ribosomal subunit PS3 antibody were used as described.

    Techniques: Staining, Labeling, Mutagenesis, Over Expression, Expressing, RNA In Situ Hybridization, Western Blot

    Synapse morphology and the distribution patterns of the DGluRIIA, DGluRIIB, Discs-large, and actin proteins are unchanged in the dystrophin mutants. Third-instar larval body walls were stained with anti-HRP, an antibody that labels all type I boutons ( A , B ), with anti-DGluRIIA ( C , D ), anti-DGluRIIB ( E , F ), anti-Discs-large ( G , H ), or anti-actin ( I , J ). A , C , E , G , I , Representative wild-type (wt) muscle 6/7NMJs. B , D , F , H , J , Representative dys E6 (E6) muscle 6/7 NMJs. No significant changes in the distribution pattern of the examined proteins are observed in the dystrophin mutant compared with the wild-type control larvae.

    Journal: The Journal of Neuroscience

    Article Title: Dystrophin Is Required for Appropriate Retrograde Control of Neurotransmitter Release at the Drosophila Neuromuscular Junction

    doi: 10.1523/JNEUROSCI.4069-05.2006

    Figure Lengend Snippet: Synapse morphology and the distribution patterns of the DGluRIIA, DGluRIIB, Discs-large, and actin proteins are unchanged in the dystrophin mutants. Third-instar larval body walls were stained with anti-HRP, an antibody that labels all type I boutons ( A , B ), with anti-DGluRIIA ( C , D ), anti-DGluRIIB ( E , F ), anti-Discs-large ( G , H ), or anti-actin ( I , J ). A , C , E , G , I , Representative wild-type (wt) muscle 6/7NMJs. B , D , F , H , J , Representative dys E6 (E6) muscle 6/7 NMJs. No significant changes in the distribution pattern of the examined proteins are observed in the dystrophin mutant compared with the wild-type control larvae.

    Article Snippet: Anti-dysCO2 H (1:3000), anti-Dyslarge (1:3000), anti-HRP (1:500) (Promega, Madison, WI), anti-DGluRIIA (1:500) (Developmental Studies Hybridoma Bank, University of Iowa, Iowa City, IA) , anti-DGluRIIB (1:2500) , anti-Discslarge (Developmental Studies Hybridoma Bank) ( ) (1:500), anti-actin (1:5000) (MP Biomedicals, Aurora, OH), anti-Phospho-Mad ( ) (1:1000), Alexa Fluor-conjugated secondary antibodies (Invitrogen, Breda, The Netherlands), and an anti-ribosomal subunit PS3 antibody were used as described.

    Techniques: Staining, Mutagenesis