hrp conjugate  (Millipore)


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    Structured Review

    Millipore hrp conjugate
    Hrp Conjugate, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugate/product/Millipore
    Average 99 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    hrp conjugate - by Bioz Stars, 2020-02
    99/100 stars

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    Centrifugation:

    Article Title: Development of a Tetracycline-Inducible Gene Expression System for the Study of Helicobacter pylori Pathogenesis
    Article Snippet: Cells were incubated on ice for 15 min and then sonicated for 10 s. Insoluble cell debris was removed by centrifugation, and the protein concentration of the clarified supernatant was measured. .. The membrane was washed again, and detection of the secondary HRP conjugate was accomplished by using chemiluminescence (Sigma).

    Immunocytochemistry:

    Article Title: A ketogenic diet accelerates neurodegeneration in mice with induced mitochondrial DNA toxicity in the forebrain
    Article Snippet: Antibodies against the following proteins were used for immunocytochemistry at the indicated concentrations: mitochondrial complex II, 70 kDA subunit (Invitrogen, Cat-number: 459200; dilution 1:1000), voltage-dependent anion channel 1 (VDAC1; Abcam, ab15895; dilution 1:100); microtubule-associated protein 2 (MAP2; Chemicon, AB5622; dilution 1:500); glial fibrillary acidic protein (GFAP; Sigma, G3893 [monoclonal]; dilution 1:1000); superoxide dismutase 2 (SOD2; Abcam, ab13533; dilution 1:1000); cytochrome c (Cyt C; Invitrogen, 456100 [monoclonal]; dilution 1:500). .. The nitrocellulose membrane was incubated for 1 hour with secondary antibody conjugated with horseradish peroxidase (HRP) (BIO-RAD 170–6515 goat anti-rabbit IgG [H + L] HRP conjugate 1:20,000, or Sigma–Aldrich A9044 rabbit anti-mouse IgG [whole molecule] HRP conjugate 1:20,000).

    Expressing:

    Article Title: Immunohistochemical localization of translationally controlled tumor protein in the mouse digestive system
    Article Snippet: Then, the sections were treated with HRP conjugate from the ImmPRESS kit for 30 min and stained with 0.025% DAB solution (Sigma-Aldrich). .. TCTP expression (immunoreactivity) in cells was assessed as low, moderate or high based on staining intensity.

    Positive Control:

    Article Title: Proteomic divergence in Arabidopsis autopolyploids and allopolyploids and their progenitors
    Article Snippet: An aliquot (1 μg) of core histones (Millipore, Billerica, MA, USA) was included as a positive control in the western blot analysis of histone H3. .. The goat anti-rabbit IgG, horseradish peroxide conjugate (1:10 000) (Millipore) was used as a secondary antibody for immunodetection, which was performed using a SuperSignal West Pico kit (Thermo Scientific) and quantified using ImageJ software (National Institutes of Health, Bethesda, MD, USA).

    Blocking Assay:

    Article Title: Proteomic divergence in Arabidopsis autopolyploids and allopolyploids and their progenitors
    Article Snippet: For western blotting, the membrane was incubated at room temperature for 1.5 h in a protein-free blocking buffer (Thermo Scientific, Waltham, MA, USA) containing 0.05% Tween-20. .. The goat anti-rabbit IgG, horseradish peroxide conjugate (1:10 000) (Millipore) was used as a secondary antibody for immunodetection, which was performed using a SuperSignal West Pico kit (Thermo Scientific) and quantified using ImageJ software (National Institutes of Health, Bethesda, MD, USA).

    Article Title: Immunohistochemical localization of translationally controlled tumor protein in the mouse digestive system
    Article Snippet: Blocking of non-specific protein binding and immunostaining were performed with the ImmPress Reagent kit (Vector laboratories) according to the manufacturer's instructions. .. Then, the sections were treated with HRP conjugate from the ImmPRESS kit for 30 min and stained with 0.025% DAB solution (Sigma-Aldrich).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Characterization of DNA topoisomerase I in three SN-38 resistant human colon cancer cell lines reveals a new pair of resistance-associated mutations
    Article Snippet: Paragraph title: Top1 ELISA assay ... Probe antibody and HRP-conjugate were pre-incubated with mouse serum (Sigma Aldrich, 1:1000) to lower background signal.

    Incubation:

    Article Title: Proteomic divergence in Arabidopsis autopolyploids and allopolyploids and their progenitors
    Article Snippet: For western blotting, the membrane was incubated at room temperature for 1.5 h in a protein-free blocking buffer (Thermo Scientific, Waltham, MA, USA) containing 0.05% Tween-20. .. The goat anti-rabbit IgG, horseradish peroxide conjugate (1:10 000) (Millipore) was used as a secondary antibody for immunodetection, which was performed using a SuperSignal West Pico kit (Thermo Scientific) and quantified using ImageJ software (National Institutes of Health, Bethesda, MD, USA).

    Article Title: Development and Validation of an Immunoassay for Quantification of Topoisomerase I in Solid Tumor Tissues
    Article Snippet: Probe antibody and HRP-conjugate were pre-incubated with mouse serum (Sigma, 1∶1000) to lower background signal. .. Samples and standards were diluted in PBS-casein and incubated overnight at 2°C to 8°C.

    Article Title: Immunohistochemical localization of translationally controlled tumor protein in the mouse digestive system
    Article Snippet: Endogenous peroxidase was blocked by incubation with 1% hydrogen peroxide for 15 min. .. Then, the sections were treated with HRP conjugate from the ImmPRESS kit for 30 min and stained with 0.025% DAB solution (Sigma-Aldrich).

    Article Title: A ketogenic diet accelerates neurodegeneration in mice with induced mitochondrial DNA toxicity in the forebrain
    Article Snippet: .. The nitrocellulose membrane was incubated for 1 hour with secondary antibody conjugated with horseradish peroxidase (HRP) (BIO-RAD 170–6515 goat anti-rabbit IgG [H + L] HRP conjugate 1:20,000, or Sigma–Aldrich A9044 rabbit anti-mouse IgG [whole molecule] HRP conjugate 1:20,000). .. Notably, all protein targets were developed on the same membrane followed by consecutive stripping of the membrane for 30 minutes using stripping buffer (Thermo Scientific).

    Article Title: HB-GAM (pleiotrophin) reverses inhibition of neural regeneration by the CNS extracellular matrix
    Article Snippet: The PTPσ recombinants (80 nM) were mixed with different concentrations of recombinant HB-GAM in 1% BSA and 0.05% Tween-20 in PBS, and the mixture was incubated in the aggrecan-coated wells overnight at 4 °C. .. The enzymatic activity of the bound HRP conjugate was measured at 450 nm by using O-phenylendiamine dihydrochloride (OPD, SIGMA P9187).

    Article Title: Development of a Tetracycline-Inducible Gene Expression System for the Study of Helicobacter pylori Pathogenesis
    Article Snippet: The proteins were separated by 10% SDS-PAGE and transferred onto a polyvinylidene difluoride (PVDF) membrane (0.22-μm Immobilon membrane; Millipore) at 4°C with a constant voltage of 90 V in transfer buffer (192 mM glycine, 25 mM Tris, 20% [vol/vol] methanol) for 2 h. The membrane was blocked by using 2% bovine serum albumin (BSA) (Sigma) in PBST (PBS [pH 7.4], 0.1% [vol/vol] Tween 20) and then incubated with the appropriate primary antibody, washed, and incubated with a horseradish peroxidase (HRP)-conjugated secondary antibody. .. The membrane was washed again, and detection of the secondary HRP conjugate was accomplished by using chemiluminescence (Sigma).

    Article Title: Characterization of DNA topoisomerase I in three SN-38 resistant human colon cancer cell lines reveals a new pair of resistance-associated mutations
    Article Snippet: Samples and standards were diluted in PBS-casein and incubated overnight at 2 °C to 8 °C. .. Probe antibody and HRP-conjugate were pre-incubated with mouse serum (Sigma Aldrich, 1:1000) to lower background signal.

    Article Title: Design and characterization of a direct ELISA for the detection and quantification of leucomalachite green
    Article Snippet: .. The reaction mixture was incubated overnight at 4°C and then added drop wise to a solution of HRP (10 mg in 1 ml of PBS) and allowed to react at 4°C for an additional 24 h. Excess reagents were removed by dialysis against PBS for 2 days and the final HRP conjugate was concentrated using Centricon YM 30-kDa centrifugal filters (Millipore Corporation, Bedford, MA, USA) at 3500 rpm and 4°C. .. The concentrated conjugate was further purified by gel chromatography using Sephadex® G-100 (Sigma-Aldrich) and PBS as the mobile phase.

    Stripping Membranes:

    Article Title: A ketogenic diet accelerates neurodegeneration in mice with induced mitochondrial DNA toxicity in the forebrain
    Article Snippet: The nitrocellulose membrane was incubated for 1 hour with secondary antibody conjugated with horseradish peroxidase (HRP) (BIO-RAD 170–6515 goat anti-rabbit IgG [H + L] HRP conjugate 1:20,000, or Sigma–Aldrich A9044 rabbit anti-mouse IgG [whole molecule] HRP conjugate 1:20,000). .. Notably, all protein targets were developed on the same membrane followed by consecutive stripping of the membrane for 30 minutes using stripping buffer (Thermo Scientific).

    Activity Assay:

    Article Title: HB-GAM (pleiotrophin) reverses inhibition of neural regeneration by the CNS extracellular matrix
    Article Snippet: .. The enzymatic activity of the bound HRP conjugate was measured at 450 nm by using O-phenylendiamine dihydrochloride (OPD, SIGMA P9187). .. Surface plasmon resonance studies were conducted with a Biacore T100 instrument (GE healthcare).

    Mass Spectrometry:

    Article Title: Ubiquitin Chain Enrichment Middle-Down Mass Spectrometry (UbiChEM-MS) Reveals Cell-Cycle Dependent Formation of Lys11/Lys48 Branched Ubiquitin Chains
    Article Snippet: .. The following materials were used in this study: pFUSEss-CHIg-hG1 (InvivoGen), pFUSE2ss-CLIg-hk (InvivoGen), Xfect transfection reagent (Takara Clontech; 631318), DMEM, high glucose (ThermoFisher Scientific; 11965118), Fetal Bovine Serum, qualified, USDA-approved regions (ThermoFisher Scientific; 10437028), Super Low IgG Fetal Bovine Serum, US Origin (VWR; 95042), GlutaMAX supplement (ThermoFisher Scientific; 35050061), Trypsin-EDTA (0.25%), phenol red (ThermoFisher Scientific; 25200056), Chloroacetamide (Sigma-Aldrich; C0267), N-Ethylmaleimide (Alfa Aesar; AA4052606), PR619 DUB Inhibitor (Fisher Scientific; NC0818370), MG132 (Enzo Life Sciences; BML-PI102), ProBlockTM gold mammalian protease inhibitor cocktail (Goldbio; GB-331–5), Trypsin Gold, mass spectrometry grade (Promega; V5280), Ubiquitin monoclonal antibody, P4D1 (Enzo Life Sciences; BML-PW0930), Goat antimouse IgG antibody, HRP conjugate (EMD Millipore; 12–349), and Goat antihuman IgG antibody, HRP conjugate (EMD-Millipore; AP112P). .. HEK293 cells were grown in media containing DMEM, 10% (v/v) FBS, 1× glutamax, 100 U/mL penicillin, and 100 mg/mL streptomycin, at 37 °C, 5% CO2 .

    BIA-KA:

    Article Title: Characterization of DNA topoisomerase I in three SN-38 resistant human colon cancer cell lines reveals a new pair of resistance-associated mutations
    Article Snippet: Cell pellets were sonicated in lysis buffer and protein concentration was determined by BCA assay. .. Probe antibody and HRP-conjugate were pre-incubated with mouse serum (Sigma Aldrich, 1:1000) to lower background signal.

    Western Blot:

    Article Title: Proteomic divergence in Arabidopsis autopolyploids and allopolyploids and their progenitors
    Article Snippet: Paragraph title: SDS-PAGE and western blot analysis ... The goat anti-rabbit IgG, horseradish peroxide conjugate (1:10 000) (Millipore) was used as a secondary antibody for immunodetection, which was performed using a SuperSignal West Pico kit (Thermo Scientific) and quantified using ImageJ software (National Institutes of Health, Bethesda, MD, USA).

    Article Title: Normal DNA Methylation Dynamics in DICER1-Deficient Mouse Embryonic Stem Cells
    Article Snippet: Paragraph title: Immunoblots and immunofluorescence ... Secondary antisera: Goat Anti-mouse IgG, HRP conjugate (Millipore; 12-349) or goat Anti-rabbit IgG, HRP conjugate (Millipore; 12-348).

    Article Title: Development of a Tetracycline-Inducible Gene Expression System for the Study of Helicobacter pylori Pathogenesis
    Article Snippet: Paragraph title: SDS-PAGE and Western blot analysis. ... The membrane was washed again, and detection of the secondary HRP conjugate was accomplished by using chemiluminescence (Sigma).

    Conjugation Assay:

    Article Title: Design and characterization of a direct ELISA for the detection and quantification of leucomalachite green
    Article Snippet: Paragraph title: Conjugation of monodesmethyl-leucomalachite-hemisuccinate with HRP (M-LMG-HS-HRP) ... The reaction mixture was incubated overnight at 4°C and then added drop wise to a solution of HRP (10 mg in 1 ml of PBS) and allowed to react at 4°C for an additional 24 h. Excess reagents were removed by dialysis against PBS for 2 days and the final HRP conjugate was concentrated using Centricon YM 30-kDa centrifugal filters (Millipore Corporation, Bedford, MA, USA) at 3500 rpm and 4°C.

    Transfection:

    Article Title: Ubiquitin Chain Enrichment Middle-Down Mass Spectrometry (UbiChEM-MS) Reveals Cell-Cycle Dependent Formation of Lys11/Lys48 Branched Ubiquitin Chains
    Article Snippet: .. The following materials were used in this study: pFUSEss-CHIg-hG1 (InvivoGen), pFUSE2ss-CLIg-hk (InvivoGen), Xfect transfection reagent (Takara Clontech; 631318), DMEM, high glucose (ThermoFisher Scientific; 11965118), Fetal Bovine Serum, qualified, USDA-approved regions (ThermoFisher Scientific; 10437028), Super Low IgG Fetal Bovine Serum, US Origin (VWR; 95042), GlutaMAX supplement (ThermoFisher Scientific; 35050061), Trypsin-EDTA (0.25%), phenol red (ThermoFisher Scientific; 25200056), Chloroacetamide (Sigma-Aldrich; C0267), N-Ethylmaleimide (Alfa Aesar; AA4052606), PR619 DUB Inhibitor (Fisher Scientific; NC0818370), MG132 (Enzo Life Sciences; BML-PI102), ProBlockTM gold mammalian protease inhibitor cocktail (Goldbio; GB-331–5), Trypsin Gold, mass spectrometry grade (Promega; V5280), Ubiquitin monoclonal antibody, P4D1 (Enzo Life Sciences; BML-PW0930), Goat antimouse IgG antibody, HRP conjugate (EMD Millipore; 12–349), and Goat antihuman IgG antibody, HRP conjugate (EMD-Millipore; AP112P). .. HEK293 cells were grown in media containing DMEM, 10% (v/v) FBS, 1× glutamax, 100 U/mL penicillin, and 100 mg/mL streptomycin, at 37 °C, 5% CO2 .

    Chromatography:

    Article Title: Design and characterization of a direct ELISA for the detection and quantification of leucomalachite green
    Article Snippet: The reaction mixture was incubated overnight at 4°C and then added drop wise to a solution of HRP (10 mg in 1 ml of PBS) and allowed to react at 4°C for an additional 24 h. Excess reagents were removed by dialysis against PBS for 2 days and the final HRP conjugate was concentrated using Centricon YM 30-kDa centrifugal filters (Millipore Corporation, Bedford, MA, USA) at 3500 rpm and 4°C. .. The concentrated conjugate was further purified by gel chromatography using Sephadex® G-100 (Sigma-Aldrich) and PBS as the mobile phase.

    Protease Inhibitor:

    Article Title: Ubiquitin Chain Enrichment Middle-Down Mass Spectrometry (UbiChEM-MS) Reveals Cell-Cycle Dependent Formation of Lys11/Lys48 Branched Ubiquitin Chains
    Article Snippet: .. The following materials were used in this study: pFUSEss-CHIg-hG1 (InvivoGen), pFUSE2ss-CLIg-hk (InvivoGen), Xfect transfection reagent (Takara Clontech; 631318), DMEM, high glucose (ThermoFisher Scientific; 11965118), Fetal Bovine Serum, qualified, USDA-approved regions (ThermoFisher Scientific; 10437028), Super Low IgG Fetal Bovine Serum, US Origin (VWR; 95042), GlutaMAX supplement (ThermoFisher Scientific; 35050061), Trypsin-EDTA (0.25%), phenol red (ThermoFisher Scientific; 25200056), Chloroacetamide (Sigma-Aldrich; C0267), N-Ethylmaleimide (Alfa Aesar; AA4052606), PR619 DUB Inhibitor (Fisher Scientific; NC0818370), MG132 (Enzo Life Sciences; BML-PI102), ProBlockTM gold mammalian protease inhibitor cocktail (Goldbio; GB-331–5), Trypsin Gold, mass spectrometry grade (Promega; V5280), Ubiquitin monoclonal antibody, P4D1 (Enzo Life Sciences; BML-PW0930), Goat antimouse IgG antibody, HRP conjugate (EMD Millipore; 12–349), and Goat antihuman IgG antibody, HRP conjugate (EMD-Millipore; AP112P). .. HEK293 cells were grown in media containing DMEM, 10% (v/v) FBS, 1× glutamax, 100 U/mL penicillin, and 100 mg/mL streptomycin, at 37 °C, 5% CO2 .

    Article Title: A ketogenic diet accelerates neurodegeneration in mice with induced mitochondrial DNA toxicity in the forebrain
    Article Snippet: Hippocampus of wild-type, mutUNG1, and mutUNG1-keto mice was homogenized in lysis buffer containing 0.5% Igepal CA-630 (NP-40), 2-mM dithiothreitol, 1% protease inhibitor cocktail, and 0.5% Triton X-100 in PBS, and total protein concentration was measured using Direct DetectTM (Millipore). .. The nitrocellulose membrane was incubated for 1 hour with secondary antibody conjugated with horseradish peroxidase (HRP) (BIO-RAD 170–6515 goat anti-rabbit IgG [H + L] HRP conjugate 1:20,000, or Sigma–Aldrich A9044 rabbit anti-mouse IgG [whole molecule] HRP conjugate 1:20,000).

    SDS Page:

    Article Title: Proteomic divergence in Arabidopsis autopolyploids and allopolyploids and their progenitors
    Article Snippet: Paragraph title: SDS-PAGE and western blot analysis ... The goat anti-rabbit IgG, horseradish peroxide conjugate (1:10 000) (Millipore) was used as a secondary antibody for immunodetection, which was performed using a SuperSignal West Pico kit (Thermo Scientific) and quantified using ImageJ software (National Institutes of Health, Bethesda, MD, USA).

    Article Title: A ketogenic diet accelerates neurodegeneration in mice with induced mitochondrial DNA toxicity in the forebrain
    Article Snippet: In brief, the proteins in SDS-PAGE gel were transferred onto the nitrocellulose membrane and incubated with primary antibody against SOD2 (Abcam ab13533; 1:5000), VDAC1 (Abam ab15895; 1:1000), sirtuin 1 (SIRT1; Sigma AV32386; 1:2500), mitochondrial fission 1 protein (FIS1; Sigma HPA017430; 1:1000), and β-actin (ActB; Milipore MAB1501R; 1:5000). .. The nitrocellulose membrane was incubated for 1 hour with secondary antibody conjugated with horseradish peroxidase (HRP) (BIO-RAD 170–6515 goat anti-rabbit IgG [H + L] HRP conjugate 1:20,000, or Sigma–Aldrich A9044 rabbit anti-mouse IgG [whole molecule] HRP conjugate 1:20,000).

    Article Title: Development of a Tetracycline-Inducible Gene Expression System for the Study of Helicobacter pylori Pathogenesis
    Article Snippet: Paragraph title: SDS-PAGE and Western blot analysis. ... The membrane was washed again, and detection of the secondary HRP conjugate was accomplished by using chemiluminescence (Sigma).

    Light Microscopy:

    Article Title: A ketogenic diet accelerates neurodegeneration in mice with induced mitochondrial DNA toxicity in the forebrain
    Article Snippet: Paragraph title: 2.2. Light microscopy and biochemistry ... The nitrocellulose membrane was incubated for 1 hour with secondary antibody conjugated with horseradish peroxidase (HRP) (BIO-RAD 170–6515 goat anti-rabbit IgG [H + L] HRP conjugate 1:20,000, or Sigma–Aldrich A9044 rabbit anti-mouse IgG [whole molecule] HRP conjugate 1:20,000).

    Purification:

    Article Title: Design and characterization of a direct ELISA for the detection and quantification of leucomalachite green
    Article Snippet: The reaction mixture was incubated overnight at 4°C and then added drop wise to a solution of HRP (10 mg in 1 ml of PBS) and allowed to react at 4°C for an additional 24 h. Excess reagents were removed by dialysis against PBS for 2 days and the final HRP conjugate was concentrated using Centricon YM 30-kDa centrifugal filters (Millipore Corporation, Bedford, MA, USA) at 3500 rpm and 4°C. .. The concentrated conjugate was further purified by gel chromatography using Sephadex® G-100 (Sigma-Aldrich) and PBS as the mobile phase.

    Protein Concentration:

    Article Title: A ketogenic diet accelerates neurodegeneration in mice with induced mitochondrial DNA toxicity in the forebrain
    Article Snippet: Hippocampus of wild-type, mutUNG1, and mutUNG1-keto mice was homogenized in lysis buffer containing 0.5% Igepal CA-630 (NP-40), 2-mM dithiothreitol, 1% protease inhibitor cocktail, and 0.5% Triton X-100 in PBS, and total protein concentration was measured using Direct DetectTM (Millipore). .. The nitrocellulose membrane was incubated for 1 hour with secondary antibody conjugated with horseradish peroxidase (HRP) (BIO-RAD 170–6515 goat anti-rabbit IgG [H + L] HRP conjugate 1:20,000, or Sigma–Aldrich A9044 rabbit anti-mouse IgG [whole molecule] HRP conjugate 1:20,000).

    Article Title: Development of a Tetracycline-Inducible Gene Expression System for the Study of Helicobacter pylori Pathogenesis
    Article Snippet: Cells were incubated on ice for 15 min and then sonicated for 10 s. Insoluble cell debris was removed by centrifugation, and the protein concentration of the clarified supernatant was measured. .. The membrane was washed again, and detection of the secondary HRP conjugate was accomplished by using chemiluminescence (Sigma).

    Article Title: Characterization of DNA topoisomerase I in three SN-38 resistant human colon cancer cell lines reveals a new pair of resistance-associated mutations
    Article Snippet: Cell pellets were sonicated in lysis buffer and protein concentration was determined by BCA assay. .. Probe antibody and HRP-conjugate were pre-incubated with mouse serum (Sigma Aldrich, 1:1000) to lower background signal.

    Sonication:

    Article Title: Development of a Tetracycline-Inducible Gene Expression System for the Study of Helicobacter pylori Pathogenesis
    Article Snippet: Cells were incubated on ice for 15 min and then sonicated for 10 s. Insoluble cell debris was removed by centrifugation, and the protein concentration of the clarified supernatant was measured. .. The membrane was washed again, and detection of the secondary HRP conjugate was accomplished by using chemiluminescence (Sigma).

    Article Title: Characterization of DNA topoisomerase I in three SN-38 resistant human colon cancer cell lines reveals a new pair of resistance-associated mutations
    Article Snippet: Cell pellets were sonicated in lysis buffer and protein concentration was determined by BCA assay. .. Probe antibody and HRP-conjugate were pre-incubated with mouse serum (Sigma Aldrich, 1:1000) to lower background signal.

    Binding Assay:

    Article Title: HB-GAM (pleiotrophin) reverses inhibition of neural regeneration by the CNS extracellular matrix
    Article Snippet: Paragraph title: Solid phase binding assays ... The enzymatic activity of the bound HRP conjugate was measured at 450 nm by using O-phenylendiamine dihydrochloride (OPD, SIGMA P9187).

    Immunofluorescence:

    Article Title: Normal DNA Methylation Dynamics in DICER1-Deficient Mouse Embryonic Stem Cells
    Article Snippet: Paragraph title: Immunoblots and immunofluorescence ... Secondary antisera: Goat Anti-mouse IgG, HRP conjugate (Millipore; 12-349) or goat Anti-rabbit IgG, HRP conjugate (Millipore; 12-348).

    Mutagenesis:

    Article Title: HB-GAM (pleiotrophin) reverses inhibition of neural regeneration by the CNS extracellular matrix
    Article Snippet: The PTPσ WT and the PTPσ mutant lacking the CS binding site were Fc fusion proteins. .. The enzymatic activity of the bound HRP conjugate was measured at 450 nm by using O-phenylendiamine dihydrochloride (OPD, SIGMA P9187).

    Immunohistochemistry:

    Article Title: Immunohistochemical localization of translationally controlled tumor protein in the mouse digestive system
    Article Snippet: Paragraph title: Immunohistochemistry ... Then, the sections were treated with HRP conjugate from the ImmPRESS kit for 30 min and stained with 0.025% DAB solution (Sigma-Aldrich).

    Microscopy:

    Article Title: Immunohistochemical localization of translationally controlled tumor protein in the mouse digestive system
    Article Snippet: Then, the sections were treated with HRP conjugate from the ImmPRESS kit for 30 min and stained with 0.025% DAB solution (Sigma-Aldrich). .. Stained sections were analyzed under the microscope (Axio Scope, A1, Zeiss), and representative photographs were taken with a digital camera.

    Immunodetection:

    Article Title: Proteomic divergence in Arabidopsis autopolyploids and allopolyploids and their progenitors
    Article Snippet: .. The goat anti-rabbit IgG, horseradish peroxide conjugate (1:10 000) (Millipore) was used as a secondary antibody for immunodetection, which was performed using a SuperSignal West Pico kit (Thermo Scientific) and quantified using ImageJ software (National Institutes of Health, Bethesda, MD, USA). ..

    Immunostaining:

    Article Title: Immunohistochemical localization of translationally controlled tumor protein in the mouse digestive system
    Article Snippet: Blocking of non-specific protein binding and immunostaining were performed with the ImmPress Reagent kit (Vector laboratories) according to the manufacturer's instructions. .. Then, the sections were treated with HRP conjugate from the ImmPRESS kit for 30 min and stained with 0.025% DAB solution (Sigma-Aldrich).

    Lysis:

    Article Title: A ketogenic diet accelerates neurodegeneration in mice with induced mitochondrial DNA toxicity in the forebrain
    Article Snippet: Hippocampus of wild-type, mutUNG1, and mutUNG1-keto mice was homogenized in lysis buffer containing 0.5% Igepal CA-630 (NP-40), 2-mM dithiothreitol, 1% protease inhibitor cocktail, and 0.5% Triton X-100 in PBS, and total protein concentration was measured using Direct DetectTM (Millipore). .. The nitrocellulose membrane was incubated for 1 hour with secondary antibody conjugated with horseradish peroxidase (HRP) (BIO-RAD 170–6515 goat anti-rabbit IgG [H + L] HRP conjugate 1:20,000, or Sigma–Aldrich A9044 rabbit anti-mouse IgG [whole molecule] HRP conjugate 1:20,000).

    Article Title: Development of a Tetracycline-Inducible Gene Expression System for the Study of Helicobacter pylori Pathogenesis
    Article Snippet: Bacterial whole-cell lysates were prepared by resuspending washed bacteria in ice-cold Tris lysis buffer (50 mM Tris [pH 7.0], 250 mM NaCl, 1.0% [vol/vol] Triton X-100, 100 μM phenylmethylsulfonyl fluoride [PMSF]). .. The membrane was washed again, and detection of the secondary HRP conjugate was accomplished by using chemiluminescence (Sigma).

    Article Title: Characterization of DNA topoisomerase I in three SN-38 resistant human colon cancer cell lines reveals a new pair of resistance-associated mutations
    Article Snippet: Cell pellets were sonicated in lysis buffer and protein concentration was determined by BCA assay. .. Probe antibody and HRP-conjugate were pre-incubated with mouse serum (Sigma Aldrich, 1:1000) to lower background signal.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: A ketogenic diet accelerates neurodegeneration in mice with induced mitochondrial DNA toxicity in the forebrain
    Article Snippet: Antibodies against the following proteins were used for immunocytochemistry at the indicated concentrations: mitochondrial complex II, 70 kDA subunit (Invitrogen, Cat-number: 459200; dilution 1:1000), voltage-dependent anion channel 1 (VDAC1; Abcam, ab15895; dilution 1:100); microtubule-associated protein 2 (MAP2; Chemicon, AB5622; dilution 1:500); glial fibrillary acidic protein (GFAP; Sigma, G3893 [monoclonal]; dilution 1:1000); superoxide dismutase 2 (SOD2; Abcam, ab13533; dilution 1:1000); cytochrome c (Cyt C; Invitrogen, 456100 [monoclonal]; dilution 1:500). .. The nitrocellulose membrane was incubated for 1 hour with secondary antibody conjugated with horseradish peroxidase (HRP) (BIO-RAD 170–6515 goat anti-rabbit IgG [H + L] HRP conjugate 1:20,000, or Sigma–Aldrich A9044 rabbit anti-mouse IgG [whole molecule] HRP conjugate 1:20,000).

    Mouse Assay:

    Article Title: A ketogenic diet accelerates neurodegeneration in mice with induced mitochondrial DNA toxicity in the forebrain
    Article Snippet: Hippocampus of wild-type, mutUNG1, and mutUNG1-keto mice was homogenized in lysis buffer containing 0.5% Igepal CA-630 (NP-40), 2-mM dithiothreitol, 1% protease inhibitor cocktail, and 0.5% Triton X-100 in PBS, and total protein concentration was measured using Direct DetectTM (Millipore). .. The nitrocellulose membrane was incubated for 1 hour with secondary antibody conjugated with horseradish peroxidase (HRP) (BIO-RAD 170–6515 goat anti-rabbit IgG [H + L] HRP conjugate 1:20,000, or Sigma–Aldrich A9044 rabbit anti-mouse IgG [whole molecule] HRP conjugate 1:20,000).

    SPR Assay:

    Article Title: HB-GAM (pleiotrophin) reverses inhibition of neural regeneration by the CNS extracellular matrix
    Article Snippet: The enzymatic activity of the bound HRP conjugate was measured at 450 nm by using O-phenylendiamine dihydrochloride (OPD, SIGMA P9187). .. Surface plasmon resonance studies were conducted with a Biacore T100 instrument (GE healthcare).

    Software:

    Article Title: Proteomic divergence in Arabidopsis autopolyploids and allopolyploids and their progenitors
    Article Snippet: .. The goat anti-rabbit IgG, horseradish peroxide conjugate (1:10 000) (Millipore) was used as a secondary antibody for immunodetection, which was performed using a SuperSignal West Pico kit (Thermo Scientific) and quantified using ImageJ software (National Institutes of Health, Bethesda, MD, USA). ..

    Recombinant:

    Article Title: HB-GAM (pleiotrophin) reverses inhibition of neural regeneration by the CNS extracellular matrix
    Article Snippet: The PTPσ recombinants (80 nM) were mixed with different concentrations of recombinant HB-GAM in 1% BSA and 0.05% Tween-20 in PBS, and the mixture was incubated in the aggrecan-coated wells overnight at 4 °C. .. The enzymatic activity of the bound HRP conjugate was measured at 450 nm by using O-phenylendiamine dihydrochloride (OPD, SIGMA P9187).

    Protein Binding:

    Article Title: Immunohistochemical localization of translationally controlled tumor protein in the mouse digestive system
    Article Snippet: Blocking of non-specific protein binding and immunostaining were performed with the ImmPress Reagent kit (Vector laboratories) according to the manufacturer's instructions. .. Then, the sections were treated with HRP conjugate from the ImmPRESS kit for 30 min and stained with 0.025% DAB solution (Sigma-Aldrich).

    Staining:

    Article Title: Immunohistochemical localization of translationally controlled tumor protein in the mouse digestive system
    Article Snippet: .. Then, the sections were treated with HRP conjugate from the ImmPRESS kit for 30 min and stained with 0.025% DAB solution (Sigma-Aldrich). .. Cell nuclei were stained with Harris hematoxylin (Sigma-Aldrich) and sections were embedded with Permount (Fisher Scientific).

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  • 99
    Millipore anti mouse immunoglobulin g igg horse radish peroxidase hrpo conjugate
    Purification and preliminary characterization of recombinant E1E2 bv . (A) Ni 2+ -NTA affinity chromatographic purification of E1E2 bv . The solid blue curve represents the UV absorbance at 280 nm. The imidazole step gradient is shown by the dashed gray line. (B) SDS–PAGE analysis of the purified material (pooled peak fractions from ‘ A ’) visualized by Coomassie blue staining. (C) Western blot of the purified preparation using mAb 24A12 in conjunction with anti-mouse <t>IgG-HRPO.</t> (D) Protein blot showing the interaction of the purified material with Con A-HRPO. Low molecular weight protein markers (B) or pre-stained protein markers (C,D) were run in lanes marked ‘M’; their sizes (kDa) are shown to the left of each panel. The position of E1E2 bv is shown by the arrow on the right side of (B–D) .
    Anti Mouse Immunoglobulin G Igg Horse Radish Peroxidase Hrpo Conjugate, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 2 article reviews
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    86
    Millipore goat anti mouse immunoglobulin g igg horseradish peroxidase conjugate
    Virion-specific <t>IgG</t> and neutralizing-antibody responses in the DNA primed/FI-MCMV-boosted or live virus-vaccinated mice. Mice from each group vaccinated with either pc3-Ua plus PBS plus alum (two mice per group), All-U pDNA plus FI+alum (six mice
    Goat Anti Mouse Immunoglobulin G Igg Horseradish Peroxidase Conjugate, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse immunoglobulin g igg horseradish peroxidase conjugate/product/Millipore
    Average 86 stars, based on 2 article reviews
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    99
    Millipore anti human hrp
    Competition ELISA analysis of interactions of lectin and lectin-DAVEI proteins ( A ) Direct binding of CVN and CVN-DAVEI-L2-3Trp to gp120 immobilized on ELISA plate in the presence of <t>2G12</t> prebound to gp120. 2G12 bound to gp120 was detected using anti-human <t>HRP</t> (data not shown). ( B ) Loss of binding of 2G12 to gp120 immobilized in the presence of increasing concentrations of CVN and CVN-DAVEI-L2-3Trp. ( C ) Binding of CVN and CVN-DAVEI-L2-3Trp to immobilized gp120 in the presence of increasing concentrations of 2G12. No loss of CVN and CVN-DAVEI-L2-3Trp was seen. ( D ) Loss of binding of 2G12 to gp120 immobilized in the presence of increasing concentrations of MVN, MVN-DAVEI2-3Trp and MVN-DAVEI2-3Trp(Q81K/M83R). ( E ) Detection of binding of 2G12 (with increasing concentrations) to gp120 captured with immobilized CVN-DAVEI-L2-3Trp (positive control), MVN, MVN-DAVEI2-3Trp and MVN-DAVEI2-3Trp(Q81K/M83R) on ELISA plate ( n = 3).
    Anti Human Hrp, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Purification and preliminary characterization of recombinant E1E2 bv . (A) Ni 2+ -NTA affinity chromatographic purification of E1E2 bv . The solid blue curve represents the UV absorbance at 280 nm. The imidazole step gradient is shown by the dashed gray line. (B) SDS–PAGE analysis of the purified material (pooled peak fractions from ‘ A ’) visualized by Coomassie blue staining. (C) Western blot of the purified preparation using mAb 24A12 in conjunction with anti-mouse IgG-HRPO. (D) Protein blot showing the interaction of the purified material with Con A-HRPO. Low molecular weight protein markers (B) or pre-stained protein markers (C,D) were run in lanes marked ‘M’; their sizes (kDa) are shown to the left of each panel. The position of E1E2 bv is shown by the arrow on the right side of (B–D) .

    Journal: Frontiers in Microbiology

    Article Title: Pichia pastoris-Expressed Bivalent Virus-Like Particulate Vaccine Induces Domain III-Focused Bivalent Neutralizing Antibodies without Antibody-Dependent Enhancement in Vivo

    doi: 10.3389/fmicb.2017.02644

    Figure Lengend Snippet: Purification and preliminary characterization of recombinant E1E2 bv . (A) Ni 2+ -NTA affinity chromatographic purification of E1E2 bv . The solid blue curve represents the UV absorbance at 280 nm. The imidazole step gradient is shown by the dashed gray line. (B) SDS–PAGE analysis of the purified material (pooled peak fractions from ‘ A ’) visualized by Coomassie blue staining. (C) Western blot of the purified preparation using mAb 24A12 in conjunction with anti-mouse IgG-HRPO. (D) Protein blot showing the interaction of the purified material with Con A-HRPO. Low molecular weight protein markers (B) or pre-stained protein markers (C,D) were run in lanes marked ‘M’; their sizes (kDa) are shown to the left of each panel. The position of E1E2 bv is shown by the arrow on the right side of (B–D) .

    Article Snippet: Anti-mouse immunoglobulin G (IgG)-horse radish peroxidase (HRPO) conjugate for ELISA was purchased from Calbiochem, San Diego, CA, United States.

    Techniques: Purification, Recombinant, SDS Page, Staining, Western Blot, Molecular Weight

    Virion-specific IgG and neutralizing-antibody responses in the DNA primed/FI-MCMV-boosted or live virus-vaccinated mice. Mice from each group vaccinated with either pc3-Ua plus PBS plus alum (two mice per group), All-U pDNA plus FI+alum (six mice

    Journal:

    Article Title: Systemic Priming-Boosting Immunization with a Trivalent Plasmid DNA and Inactivated Murine Cytomegalovirus (MCMV) Vaccine Provides Long-Term Protection against Viral Replication following Systemic or Mucosal MCMV Challenge

    doi: 10.1128/JVI.79.1.159-175.2005

    Figure Lengend Snippet: Virion-specific IgG and neutralizing-antibody responses in the DNA primed/FI-MCMV-boosted or live virus-vaccinated mice. Mice from each group vaccinated with either pc3-Ua plus PBS plus alum (two mice per group), All-U pDNA plus FI+alum (six mice

    Article Snippet: To detect gB, blocked blots were incubated with the monoclonal antibody 2E8.12A (a gift from Lambert Loh, University of Saskatchewan, Saskatoon, Canada), and bound antibody was detected using a goat-anti-mouse immunoglobulin G (IgG) horseradish peroxidase conjugate (Calbiochem) and enhanced chemiluminescence (Supersignal West Pico; Pierce).

    Techniques: Mouse Assay

    Virion-specific IgG and neutralizing-antibody responses in vaccinated mice. On the weeks of the experiment shown in Fig. , four to eight mice per vaccine group were retroorbitally bled and sera were prepared. Arrows and numbers indicate

    Journal:

    Article Title: Systemic Priming-Boosting Immunization with a Trivalent Plasmid DNA and Inactivated Murine Cytomegalovirus (MCMV) Vaccine Provides Long-Term Protection against Viral Replication following Systemic or Mucosal MCMV Challenge

    doi: 10.1128/JVI.79.1.159-175.2005

    Figure Lengend Snippet: Virion-specific IgG and neutralizing-antibody responses in vaccinated mice. On the weeks of the experiment shown in Fig. , four to eight mice per vaccine group were retroorbitally bled and sera were prepared. Arrows and numbers indicate

    Article Snippet: To detect gB, blocked blots were incubated with the monoclonal antibody 2E8.12A (a gift from Lambert Loh, University of Saskatchewan, Saskatoon, Canada), and bound antibody was detected using a goat-anti-mouse immunoglobulin G (IgG) horseradish peroxidase conjugate (Calbiochem) and enhanced chemiluminescence (Supersignal West Pico; Pierce).

    Techniques: Mouse Assay

    Competition ELISA analysis of interactions of lectin and lectin-DAVEI proteins ( A ) Direct binding of CVN and CVN-DAVEI-L2-3Trp to gp120 immobilized on ELISA plate in the presence of 2G12 prebound to gp120. 2G12 bound to gp120 was detected using anti-human HRP (data not shown). ( B ) Loss of binding of 2G12 to gp120 immobilized in the presence of increasing concentrations of CVN and CVN-DAVEI-L2-3Trp. ( C ) Binding of CVN and CVN-DAVEI-L2-3Trp to immobilized gp120 in the presence of increasing concentrations of 2G12. No loss of CVN and CVN-DAVEI-L2-3Trp was seen. ( D ) Loss of binding of 2G12 to gp120 immobilized in the presence of increasing concentrations of MVN, MVN-DAVEI2-3Trp and MVN-DAVEI2-3Trp(Q81K/M83R). ( E ) Detection of binding of 2G12 (with increasing concentrations) to gp120 captured with immobilized CVN-DAVEI-L2-3Trp (positive control), MVN, MVN-DAVEI2-3Trp and MVN-DAVEI2-3Trp(Q81K/M83R) on ELISA plate ( n = 3).

    Journal: The Biochemical journal

    Article Title: Restricted HIV-1 Env glycan engagement by lectin-reengineered DAVEI protein chimera is sufficient for lytic inactivation of the virus

    doi: 10.1042/BCJ20170662

    Figure Lengend Snippet: Competition ELISA analysis of interactions of lectin and lectin-DAVEI proteins ( A ) Direct binding of CVN and CVN-DAVEI-L2-3Trp to gp120 immobilized on ELISA plate in the presence of 2G12 prebound to gp120. 2G12 bound to gp120 was detected using anti-human HRP (data not shown). ( B ) Loss of binding of 2G12 to gp120 immobilized in the presence of increasing concentrations of CVN and CVN-DAVEI-L2-3Trp. ( C ) Binding of CVN and CVN-DAVEI-L2-3Trp to immobilized gp120 in the presence of increasing concentrations of 2G12. No loss of CVN and CVN-DAVEI-L2-3Trp was seen. ( D ) Loss of binding of 2G12 to gp120 immobilized in the presence of increasing concentrations of MVN, MVN-DAVEI2-3Trp and MVN-DAVEI2-3Trp(Q81K/M83R). ( E ) Detection of binding of 2G12 (with increasing concentrations) to gp120 captured with immobilized CVN-DAVEI-L2-3Trp (positive control), MVN, MVN-DAVEI2-3Trp and MVN-DAVEI2-3Trp(Q81K/M83R) on ELISA plate ( n = 3).

    Article Snippet: 2G12 binding was also detected using anti-human HRP (dilution factor 1: 3000; Millipore — AP101P) to ensure it is not lost during the wash process (data not shown).

    Techniques: Enzyme-linked Immunosorbent Assay, Binding Assay, Positive Control