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Funakoshi hrp conjugate
Detection of the intracellular DPE-modified proteins. (A) Detection of the DPE-modified proteins in Hepa1c1c7. Confluent Hepa1c1c7 cells were pre-incubated with 0.1% DMSO or 100 µM BNPP and incubated with 100 µM DPE for indicated time periods in serum-free MEM-α. The DPE-tagged cellular proteins were detected by <t>HRP-streptavidin.</t> (B) Structural comparison of DBE with DOPAC and DPE. (C) Effect of the pre-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in Hepa1c1c7 cells. Hepa1c1c7 cells were pre-incubated with DOPAC (open circle) or DBE (closed circle) for 30 min and incubated with 25 µM DPE for 3 h in serum-free MEM-α. (D) Effect of the co-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in cell lysate. Cell lysate was incubated with 25 µM DPE in combination with DOPAC (open circle) or DBE (closed circle) in the presence of 30 U laccase for 1 h. The values represent means ±S.D. of three separate experiments.
Hrp Conjugate, supplied by Funakoshi, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hrp conjugate/product/Funakoshi
Average 90 stars, based on 1 article reviews
Price from $9.99 to $1999.99
hrp conjugate - by Bioz Stars, 2020-04
90/100 stars

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1) Product Images from "A novel tag-free probe for targeting molecules interacting with a flavonoid catabolite"

Article Title: A novel tag-free probe for targeting molecules interacting with a flavonoid catabolite

Journal: Biochemistry and Biophysics Reports

doi: 10.1016/j.bbrep.2016.06.020

Detection of the intracellular DPE-modified proteins. (A) Detection of the DPE-modified proteins in Hepa1c1c7. Confluent Hepa1c1c7 cells were pre-incubated with 0.1% DMSO or 100 µM BNPP and incubated with 100 µM DPE for indicated time periods in serum-free MEM-α. The DPE-tagged cellular proteins were detected by HRP-streptavidin. (B) Structural comparison of DBE with DOPAC and DPE. (C) Effect of the pre-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in Hepa1c1c7 cells. Hepa1c1c7 cells were pre-incubated with DOPAC (open circle) or DBE (closed circle) for 30 min and incubated with 25 µM DPE for 3 h in serum-free MEM-α. (D) Effect of the co-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in cell lysate. Cell lysate was incubated with 25 µM DPE in combination with DOPAC (open circle) or DBE (closed circle) in the presence of 30 U laccase for 1 h. The values represent means ±S.D. of three separate experiments.
Figure Legend Snippet: Detection of the intracellular DPE-modified proteins. (A) Detection of the DPE-modified proteins in Hepa1c1c7. Confluent Hepa1c1c7 cells were pre-incubated with 0.1% DMSO or 100 µM BNPP and incubated with 100 µM DPE for indicated time periods in serum-free MEM-α. The DPE-tagged cellular proteins were detected by HRP-streptavidin. (B) Structural comparison of DBE with DOPAC and DPE. (C) Effect of the pre-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in Hepa1c1c7 cells. Hepa1c1c7 cells were pre-incubated with DOPAC (open circle) or DBE (closed circle) for 30 min and incubated with 25 µM DPE for 3 h in serum-free MEM-α. (D) Effect of the co-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in cell lysate. Cell lysate was incubated with 25 µM DPE in combination with DOPAC (open circle) or DBE (closed circle) in the presence of 30 U laccase for 1 h. The values represent means ±S.D. of three separate experiments.

Techniques Used: Modification, Incubation

CuAAC reaction of DPE with an azide-linked tag molecule. (A) Formation of 1,2,3-triazole by CuAAC reaction with DPE and benzyl azide. (B) Detection of GAPDH tagged by CuAAC reaction with DPE and the azide-labeled biotin. GAPDH (1 mg/ml) was incubated with or without 50 µM DPE in the presence or absence of 30 U laccase in 70 mM sodium phosphate buffer (pH 7.2) for 1 h at 37 °C. DPE-tagged GAPDH was detected by CBB staining (left) and HRP-streptavidin (right).
Figure Legend Snippet: CuAAC reaction of DPE with an azide-linked tag molecule. (A) Formation of 1,2,3-triazole by CuAAC reaction with DPE and benzyl azide. (B) Detection of GAPDH tagged by CuAAC reaction with DPE and the azide-labeled biotin. GAPDH (1 mg/ml) was incubated with or without 50 µM DPE in the presence or absence of 30 U laccase in 70 mM sodium phosphate buffer (pH 7.2) for 1 h at 37 °C. DPE-tagged GAPDH was detected by CBB staining (left) and HRP-streptavidin (right).

Techniques Used: Labeling, Incubation, Staining

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Article Title: A novel tag-free probe for targeting molecules interacting with a flavonoid catabolite
Article Snippet: 2.1 Materials DOPAC, laccase from Rhus vernicifera , glyceraldehyde-3-phosphate dehydrogenase (GAPDH), tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA), bis(4-nitrophenyl) phosphate (BNPP) and azide-PEG3 -biotin conjugate were obtained from Sigma Aldrich (St. Louis, USA). p -Toluensulfonic acid monohydrate (PTSA), n -butanol, toluene, copper (II) sulfate pentahydrate, protease inhibitor cocktail and Chemi-Lumi One Super were purchased from nacalai tasque (Kyoto, Japan). .. Streptavidin, HRP conjugate was purchased from Funakoshi (Tokyo, Japan).

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    Funakoshi hrp conjugate
    Detection of the intracellular DPE-modified proteins. (A) Detection of the DPE-modified proteins in Hepa1c1c7. Confluent Hepa1c1c7 cells were pre-incubated with 0.1% DMSO or 100 µM BNPP and incubated with 100 µM DPE for indicated time periods in serum-free MEM-α. The DPE-tagged cellular proteins were detected by <t>HRP-streptavidin.</t> (B) Structural comparison of DBE with DOPAC and DPE. (C) Effect of the pre-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in Hepa1c1c7 cells. Hepa1c1c7 cells were pre-incubated with DOPAC (open circle) or DBE (closed circle) for 30 min and incubated with 25 µM DPE for 3 h in serum-free MEM-α. (D) Effect of the co-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in cell lysate. Cell lysate was incubated with 25 µM DPE in combination with DOPAC (open circle) or DBE (closed circle) in the presence of 30 U laccase for 1 h. The values represent means ±S.D. of three separate experiments.
    Hrp Conjugate, supplied by Funakoshi, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugate/product/Funakoshi
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hrp conjugate - by Bioz Stars, 2020-04
    90/100 stars
      Buy from Supplier

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    Funakoshi biotin hrp
    Detection of the intracellular DPE-modified proteins. (A) Detection of the DPE-modified proteins in Hepa1c1c7. Confluent Hepa1c1c7 cells were pre-incubated with 0.1% DMSO or 100 µM BNPP and incubated with 100 µM DPE for indicated time periods in serum-free MEM-α. The DPE-tagged cellular proteins were detected by <t>HRP-streptavidin.</t> (B) Structural comparison of DBE with DOPAC and DPE. (C) Effect of the pre-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in Hepa1c1c7 cells. Hepa1c1c7 cells were pre-incubated with DOPAC (open circle) or DBE (closed circle) for 30 min and incubated with 25 µM DPE for 3 h in serum-free MEM-α. (D) Effect of the co-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in cell lysate. Cell lysate was incubated with 25 µM DPE in combination with DOPAC (open circle) or DBE (closed circle) in the presence of 30 U laccase for 1 h. The values represent means ±S.D. of three separate experiments.
    Biotin Hrp, supplied by Funakoshi, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin hrp/product/Funakoshi
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotin hrp - by Bioz Stars, 2020-04
    84/100 stars
      Buy from Supplier

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    Detection of the intracellular DPE-modified proteins. (A) Detection of the DPE-modified proteins in Hepa1c1c7. Confluent Hepa1c1c7 cells were pre-incubated with 0.1% DMSO or 100 µM BNPP and incubated with 100 µM DPE for indicated time periods in serum-free MEM-α. The DPE-tagged cellular proteins were detected by HRP-streptavidin. (B) Structural comparison of DBE with DOPAC and DPE. (C) Effect of the pre-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in Hepa1c1c7 cells. Hepa1c1c7 cells were pre-incubated with DOPAC (open circle) or DBE (closed circle) for 30 min and incubated with 25 µM DPE for 3 h in serum-free MEM-α. (D) Effect of the co-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in cell lysate. Cell lysate was incubated with 25 µM DPE in combination with DOPAC (open circle) or DBE (closed circle) in the presence of 30 U laccase for 1 h. The values represent means ±S.D. of three separate experiments.

    Journal: Biochemistry and Biophysics Reports

    Article Title: A novel tag-free probe for targeting molecules interacting with a flavonoid catabolite

    doi: 10.1016/j.bbrep.2016.06.020

    Figure Lengend Snippet: Detection of the intracellular DPE-modified proteins. (A) Detection of the DPE-modified proteins in Hepa1c1c7. Confluent Hepa1c1c7 cells were pre-incubated with 0.1% DMSO or 100 µM BNPP and incubated with 100 µM DPE for indicated time periods in serum-free MEM-α. The DPE-tagged cellular proteins were detected by HRP-streptavidin. (B) Structural comparison of DBE with DOPAC and DPE. (C) Effect of the pre-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in Hepa1c1c7 cells. Hepa1c1c7 cells were pre-incubated with DOPAC (open circle) or DBE (closed circle) for 30 min and incubated with 25 µM DPE for 3 h in serum-free MEM-α. (D) Effect of the co-treatment of DOPAC or DBE with DPE on the DPE-modified protein formation in cell lysate. Cell lysate was incubated with 25 µM DPE in combination with DOPAC (open circle) or DBE (closed circle) in the presence of 30 U laccase for 1 h. The values represent means ±S.D. of three separate experiments.

    Article Snippet: Streptavidin, HRP conjugate was purchased from Funakoshi (Tokyo, Japan).

    Techniques: Modification, Incubation

    CuAAC reaction of DPE with an azide-linked tag molecule. (A) Formation of 1,2,3-triazole by CuAAC reaction with DPE and benzyl azide. (B) Detection of GAPDH tagged by CuAAC reaction with DPE and the azide-labeled biotin. GAPDH (1 mg/ml) was incubated with or without 50 µM DPE in the presence or absence of 30 U laccase in 70 mM sodium phosphate buffer (pH 7.2) for 1 h at 37 °C. DPE-tagged GAPDH was detected by CBB staining (left) and HRP-streptavidin (right).

    Journal: Biochemistry and Biophysics Reports

    Article Title: A novel tag-free probe for targeting molecules interacting with a flavonoid catabolite

    doi: 10.1016/j.bbrep.2016.06.020

    Figure Lengend Snippet: CuAAC reaction of DPE with an azide-linked tag molecule. (A) Formation of 1,2,3-triazole by CuAAC reaction with DPE and benzyl azide. (B) Detection of GAPDH tagged by CuAAC reaction with DPE and the azide-labeled biotin. GAPDH (1 mg/ml) was incubated with or without 50 µM DPE in the presence or absence of 30 U laccase in 70 mM sodium phosphate buffer (pH 7.2) for 1 h at 37 °C. DPE-tagged GAPDH was detected by CBB staining (left) and HRP-streptavidin (right).

    Article Snippet: Streptavidin, HRP conjugate was purchased from Funakoshi (Tokyo, Japan).

    Techniques: Labeling, Incubation, Staining