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Bio-Rad hrp conjugate
Hrp Conjugate, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hrp conjugate/product/Bio-Rad
Average 94 stars, based on 7 article reviews
Price from $9.99 to $1999.99
hrp conjugate - by Bioz Stars, 2020-02
94/100 stars

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Related Articles

Enzyme-linked Immunosorbent Assay:

Article Title: Cell Death and Serum Markers of Collagen Metabolism during Cardiac Remodeling in Cavia porcellus Experimentally Infected with Trypanosoma cruzi
Article Snippet: Paragraph title: ELISA test for detection of IgG subclasses to T. cruzi ... HRP-conjugate was used at a dilution of 1∶ 5000 of goat anti-guinea pig IgG1 or anti-guinea pig IgG2 (ABD Serotec, USA).

Centrifugation:

Article Title: The Motheaten Mutation Rescues B Cell Signaling and Development in CD45-deficient Mice
Article Snippet: For preparation of cell lysates from nonbiotinylated or biotinylated cells, the cells were pelleted by 0.5 min centrifugation and lysed for 15 min in 400 μl cold buffer containing 150 mM NaCl, 50 mM Tris-HCl, pH 8, 2 mM EDTA, 50 mM NaF, 1 mM PMSF, 1 mM sodium orthovanadate, 0.05% NaN3 , and 1% NP-40. .. Filters were then incubated for 2 h at room temperature with antiphosphotyrosine antibody (4G10; Upstate Biotechnology Inc., Lake Placid, NY) in Tris-buffered saline with Tween followed by the space addition of goat anti–mouse antiserum labeled with peroxidase ( Amersham Corp. , Arlington Heights, IL) and HRP conjugate (Bio Rad Labs., Hercules, CA).

Recombinant:

Article Title: Survey of immune-related, mannose/fucose-binding C-type lectin receptors reveals widely divergent sugar-binding specificities
Article Snippet: All CTLR proteins were recombinant proteins containing only the exocellular domain, to which either human Fc chain or His tag was attached. .. Goat anti-hIgG IgG and goat anti-(His tag) IgG were from US Biological (Swampscott, MA), horseradish peroxidase (HRP) conjugate of goat anti-hFc-IgG was from Pierce (Rockford, IL) and HRP conjugate of mouse anti-(His tag)-IgG was from AbD Serotec (Oxford, UK).

Concentration Assay:

Article Title: Influence of Platelet Lysate on 2D and 3D Amniotic Mesenchymal Stem Cell Cultures
Article Snippet: A primary anti-IDO Ab (0.2 mg, clone H-11, Santa Cruz Biotechnology) was detected with HRP conjugate and Clarity Max Western ECL blotting substrate (Biorad) and the chemiluminescent signal was recorded with a Chemi-Doc documentation system (Biorad) for semi-quantification. .. For internal control, purified IDO protein (Bio-Techne, R & D Systems, Minneapolis, MN) was used at a total concentration of 25–200 ng.

Protease Inhibitor:

Article Title: Titrating Gene Function in the Human Fungal Pathogen Candida albicans through Poly-Adenosine Tract Insertion
Article Snippet: In brief, cell pellets were resuspended in 200 μl lysis buffer (50 mM Tris-HCl [pH 7.5], 150 mM NaCl, 1 mM EDTA, 1% Triton X-100) with added protease inhibitor cocktail (Roche) and 0.5-mm-diameter glass beads. .. HRP conjugate was detected using a Clarity ECL Western blotting detection system (Bio-Rad).

Western Blot:

Article Title: Influence of Platelet Lysate on 2D and 3D Amniotic Mesenchymal Stem Cell Cultures
Article Snippet: .. A primary anti-IDO Ab (0.2 mg, clone H-11, Santa Cruz Biotechnology) was detected with HRP conjugate and Clarity Max Western ECL blotting substrate (Biorad) and the chemiluminescent signal was recorded with a Chemi-Doc documentation system (Biorad) for semi-quantification. .. For internal control, purified IDO protein (Bio-Techne, R & D Systems, Minneapolis, MN) was used at a total concentration of 25–200 ng.

Article Title: The Expression of Leptin and Its Receptor During Tumorigenesis of Diffuse Gliomas such as Astrocytoma and Oligodendroglioma- Grade II, III and IV (NOS)
Article Snippet: .. Then secondary antibody tagged with HRP conjugate was incubated for 1 h. The intensity of the band was quantified using ECL reagent (Clarity western ECL kit, Bio-Rad) under Chemi-documentation system (Bio-Rad XRS). .. Real-time PCR Gene expression of leptin and its receptor was analyzed using real-time PCR.

Article Title: Titrating Gene Function in the Human Fungal Pathogen Candida albicans through Poly-Adenosine Tract Insertion
Article Snippet: .. HRP conjugate was detected using a Clarity ECL Western blotting detection system (Bio-Rad). .. Blots were imaged using a G:Box Chemi XT-4 system (Syngene) and analyzed using ImageJ (NIH).

Article Title: Enhanced seed viability and lipid compositional changes during natural aging by suppressing phospholipase D? in soybean seed
Article Snippet: Paragraph title: Western blot analysis and PLD enzyme assay ... The blots were incubated in a secondary antibody (1:1000 diluted Goat anti-rabbit IgG (H+L) horseradish peroxide conjugate (Bio-Rad, CA, USA) in TBST buffer) for 1 h and then washed two times in 1X TBST followed by one wash in TBS (10 mM Tris, 140 mM NaCl, pH 7.5).

Incubation:

Article Title: Cell Death and Serum Markers of Collagen Metabolism during Cardiac Remodeling in Cavia porcellus Experimentally Infected with Trypanosoma cruzi
Article Snippet: Briefly, ELISA 96 well plates (Immulon 2, Thermo Lab systems, MA) were coated with 3.5 µg/ml (for IgG1 detection) or 2.5 µg/ml (for IgG2 detection) of epimastigote alkaline extract (EAE) antigen and incubated with guinea pig serum 1∶200 dilution. .. HRP-conjugate was used at a dilution of 1∶ 5000 of goat anti-guinea pig IgG1 or anti-guinea pig IgG2 (ABD Serotec, USA).

Article Title: The Expression of Leptin and Its Receptor During Tumorigenesis of Diffuse Gliomas such as Astrocytoma and Oligodendroglioma- Grade II, III and IV (NOS)
Article Snippet: .. Then secondary antibody tagged with HRP conjugate was incubated for 1 h. The intensity of the band was quantified using ECL reagent (Clarity western ECL kit, Bio-Rad) under Chemi-documentation system (Bio-Rad XRS). .. Real-time PCR Gene expression of leptin and its receptor was analyzed using real-time PCR.

Article Title: The Motheaten Mutation Rescues B Cell Signaling and Development in CD45-deficient Mice
Article Snippet: .. Filters were then incubated for 2 h at room temperature with antiphosphotyrosine antibody (4G10; Upstate Biotechnology Inc., Lake Placid, NY) in Tris-buffered saline with Tween followed by the space addition of goat anti–mouse antiserum labeled with peroxidase ( Amersham Corp. , Arlington Heights, IL) and HRP conjugate (Bio Rad Labs., Hercules, CA). .. Filters derived using protein lysates from biotinylated cells were also probed with HRP-avidin (Pierce Chem.

Article Title: Enhanced seed viability and lipid compositional changes during natural aging by suppressing phospholipase D? in soybean seed
Article Snippet: .. The blots were incubated in a secondary antibody (1:1000 diluted Goat anti-rabbit IgG (H+L) horseradish peroxide conjugate (Bio-Rad, CA, USA) in TBST buffer) for 1 h and then washed two times in 1X TBST followed by one wash in TBS (10 mM Tris, 140 mM NaCl, pH 7.5). .. The protein bands binding to antibodies were visualized with a HRP color development reagent (Bio-Rad, CA, USA).

Blocking Assay:

Article Title: Enhanced seed viability and lipid compositional changes during natural aging by suppressing phospholipase D? in soybean seed
Article Snippet: Briefly, non-fat milk was used for membrane blocking for 1 h with agitation at 4° C, and membranes were soaked in At PLDα antibody (1:1000 in TBST containing 10 mM Tris, 140 mM NaCl and Tween 20, pH 7.4) overnight. .. The blots were incubated in a secondary antibody (1:1000 diluted Goat anti-rabbit IgG (H+L) horseradish peroxide conjugate (Bio-Rad, CA, USA) in TBST buffer) for 1 h and then washed two times in 1X TBST followed by one wash in TBS (10 mM Tris, 140 mM NaCl, pH 7.5).

Purification:

Article Title: Survey of immune-related, mannose/fucose-binding C-type lectin receptors reveals widely divergent sugar-binding specificities
Article Snippet: The CTLR/Fc chimeras were overexpressed via the FreeStyleTM293 Expression System (Invitrogen, Carlsbad, CA) and purified by Protein A column (GE Healthcare, Piscataway, NJ). .. Goat anti-hIgG IgG and goat anti-(His tag) IgG were from US Biological (Swampscott, MA), horseradish peroxidase (HRP) conjugate of goat anti-hFc-IgG was from Pierce (Rockford, IL) and HRP conjugate of mouse anti-(His tag)-IgG was from AbD Serotec (Oxford, UK).

Article Title: Influence of Platelet Lysate on 2D and 3D Amniotic Mesenchymal Stem Cell Cultures
Article Snippet: A primary anti-IDO Ab (0.2 mg, clone H-11, Santa Cruz Biotechnology) was detected with HRP conjugate and Clarity Max Western ECL blotting substrate (Biorad) and the chemiluminescent signal was recorded with a Chemi-Doc documentation system (Biorad) for semi-quantification. .. For internal control, purified IDO protein (Bio-Techne, R & D Systems, Minneapolis, MN) was used at a total concentration of 25–200 ng.

Article Title: The Expression of Leptin and Its Receptor During Tumorigenesis of Diffuse Gliomas such as Astrocytoma and Oligodendroglioma- Grade II, III and IV (NOS)
Article Snippet: Then secondary antibody tagged with HRP conjugate was incubated for 1 h. The intensity of the band was quantified using ECL reagent (Clarity western ECL kit, Bio-Rad) under Chemi-documentation system (Bio-Rad XRS). .. Total RNA purification The biopsy tissue was soaked in RNA LATER solution and stored at -80°C until use.

Produced:

Article Title: Survey of immune-related, mannose/fucose-binding C-type lectin receptors reveals widely divergent sugar-binding specificities
Article Snippet: Langerin, BDCA-2 (CLEC4C), DCIR (CLEC4A), MCL (CLEC4D), MINCLE (CLEC4E), MDL-1 (CLEC5A) and MGL (CLEC10A), all of human kind, as Fc chimera were produced in-house ( ). .. Goat anti-hIgG IgG and goat anti-(His tag) IgG were from US Biological (Swampscott, MA), horseradish peroxidase (HRP) conjugate of goat anti-hFc-IgG was from Pierce (Rockford, IL) and HRP conjugate of mouse anti-(His tag)-IgG was from AbD Serotec (Oxford, UK).

Immunoprecipitation:

Article Title: The Motheaten Mutation Rescues B Cell Signaling and Development in CD45-deficient Mice
Article Snippet: Paragraph title: Immunoprecipitation and Immunoblotting. ... Filters were then incubated for 2 h at room temperature with antiphosphotyrosine antibody (4G10; Upstate Biotechnology Inc., Lake Placid, NY) in Tris-buffered saline with Tween followed by the space addition of goat anti–mouse antiserum labeled with peroxidase ( Amersham Corp. , Arlington Heights, IL) and HRP conjugate (Bio Rad Labs., Hercules, CA).

Enzymatic Assay:

Article Title: Enhanced seed viability and lipid compositional changes during natural aging by suppressing phospholipase D? in soybean seed
Article Snippet: Paragraph title: Western blot analysis and PLD enzyme assay ... The blots were incubated in a secondary antibody (1:1000 diluted Goat anti-rabbit IgG (H+L) horseradish peroxide conjugate (Bio-Rad, CA, USA) in TBST buffer) for 1 h and then washed two times in 1X TBST followed by one wash in TBS (10 mM Tris, 140 mM NaCl, pH 7.5).

Spectrophotometry:

Article Title: Cell Death and Serum Markers of Collagen Metabolism during Cardiac Remodeling in Cavia porcellus Experimentally Infected with Trypanosoma cruzi
Article Snippet: HRP-conjugate was used at a dilution of 1∶ 5000 of goat anti-guinea pig IgG1 or anti-guinea pig IgG2 (ABD Serotec, USA). .. The OD was determined at 492 nm using a Versa Maxmicroplate spectrophotometer (Molecular Devices Corporation, USA).

Expressing:

Article Title: The Expression of Leptin and Its Receptor During Tumorigenesis of Diffuse Gliomas such as Astrocytoma and Oligodendroglioma- Grade II, III and IV (NOS)
Article Snippet: Then secondary antibody tagged with HRP conjugate was incubated for 1 h. The intensity of the band was quantified using ECL reagent (Clarity western ECL kit, Bio-Rad) under Chemi-documentation system (Bio-Rad XRS). .. Real-time PCR Gene expression of leptin and its receptor was analyzed using real-time PCR.

Lysis:

Article Title: Titrating Gene Function in the Human Fungal Pathogen Candida albicans through Poly-Adenosine Tract Insertion
Article Snippet: In brief, cell pellets were resuspended in 200 μl lysis buffer (50 mM Tris-HCl [pH 7.5], 150 mM NaCl, 1 mM EDTA, 1% Triton X-100) with added protease inhibitor cocktail (Roche) and 0.5-mm-diameter glass beads. .. HRP conjugate was detected using a Clarity ECL Western blotting detection system (Bio-Rad).

Labeling:

Article Title: The Motheaten Mutation Rescues B Cell Signaling and Development in CD45-deficient Mice
Article Snippet: .. Filters were then incubated for 2 h at room temperature with antiphosphotyrosine antibody (4G10; Upstate Biotechnology Inc., Lake Placid, NY) in Tris-buffered saline with Tween followed by the space addition of goat anti–mouse antiserum labeled with peroxidase ( Amersham Corp. , Arlington Heights, IL) and HRP conjugate (Bio Rad Labs., Hercules, CA). .. Filters derived using protein lysates from biotinylated cells were also probed with HRP-avidin (Pierce Chem.

Real-time Polymerase Chain Reaction:

Article Title: The Expression of Leptin and Its Receptor During Tumorigenesis of Diffuse Gliomas such as Astrocytoma and Oligodendroglioma- Grade II, III and IV (NOS)
Article Snippet: Then secondary antibody tagged with HRP conjugate was incubated for 1 h. The intensity of the band was quantified using ECL reagent (Clarity western ECL kit, Bio-Rad) under Chemi-documentation system (Bio-Rad XRS). .. Real-time PCR Gene expression of leptin and its receptor was analyzed using real-time PCR.

Binding Assay:

Article Title: Enhanced seed viability and lipid compositional changes during natural aging by suppressing phospholipase D? in soybean seed
Article Snippet: The blots were incubated in a secondary antibody (1:1000 diluted Goat anti-rabbit IgG (H+L) horseradish peroxide conjugate (Bio-Rad, CA, USA) in TBST buffer) for 1 h and then washed two times in 1X TBST followed by one wash in TBS (10 mM Tris, 140 mM NaCl, pH 7.5). .. The protein bands binding to antibodies were visualized with a HRP color development reagent (Bio-Rad, CA, USA).

SDS Page:

Article Title: Influence of Platelet Lysate on 2D and 3D Amniotic Mesenchymal Stem Cell Cultures
Article Snippet: Protein was loaded on precast 4–12% polyacrylamide Bis-Tris gels in a NuPage MOPS-buffer and SDS-PAGE western blotting was performed using a XCell SureLock™ Mini-Cell and a PowerEase 500 W power supply. .. A primary anti-IDO Ab (0.2 mg, clone H-11, Santa Cruz Biotechnology) was detected with HRP conjugate and Clarity Max Western ECL blotting substrate (Biorad) and the chemiluminescent signal was recorded with a Chemi-Doc documentation system (Biorad) for semi-quantification.

Article Title: Titrating Gene Function in the Human Fungal Pathogen Candida albicans through Poly-Adenosine Tract Insertion
Article Snippet: A 50-μg volume of total protein of each sample was fractionated on a 10% SDS-PAGE Mini-Protean TGX gel (Bio-Rad), transferred to a nitrocellulose membrane, and blocked with 5% nonfat milk–TBST buffer (50 mM Tris [pH 7.5], 150 mM NaCl, 0.05% Tween 20). .. HRP conjugate was detected using a Clarity ECL Western blotting detection system (Bio-Rad).

Article Title: Enhanced seed viability and lipid compositional changes during natural aging by suppressing phospholipase D? in soybean seed
Article Snippet: Denatured protein was separated in 10% SDS-PAGE gel at 100 V until the loading dye reached the bottom of the gel. .. The blots were incubated in a secondary antibody (1:1000 diluted Goat anti-rabbit IgG (H+L) horseradish peroxide conjugate (Bio-Rad, CA, USA) in TBST buffer) for 1 h and then washed two times in 1X TBST followed by one wash in TBS (10 mM Tris, 140 mM NaCl, pH 7.5).

Derivative Assay:

Article Title: The Motheaten Mutation Rescues B Cell Signaling and Development in CD45-deficient Mice
Article Snippet: Filters were then incubated for 2 h at room temperature with antiphosphotyrosine antibody (4G10; Upstate Biotechnology Inc., Lake Placid, NY) in Tris-buffered saline with Tween followed by the space addition of goat anti–mouse antiserum labeled with peroxidase ( Amersham Corp. , Arlington Heights, IL) and HRP conjugate (Bio Rad Labs., Hercules, CA). .. Filters derived using protein lysates from biotinylated cells were also probed with HRP-avidin (Pierce Chem.

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    Bio-Rad secondary antibody
    Secondary Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/secondary antibody/product/Bio-Rad
    Average 97 stars, based on 57 article reviews
    Price from $9.99 to $1999.99
    secondary antibody - by Bioz Stars, 2020-02
    97/100 stars
      Buy from Supplier

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