hpv18  (New England Biolabs)


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  • 96
    Name:
    Hela Genomic DNA
    Description:
    Hela Genomic DNA 15 ug
    Catalog Number:
    N4006S
    Price:
    118
    Category:
    Genomic DNA
    Size:
    15 ug
    Buy from Supplier


    Structured Review

    New England Biolabs hpv18
    Hela Genomic DNA
    Hela Genomic DNA 15 ug
    https://www.bioz.com/result/hpv18/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hpv18 - by Bioz Stars, 2021-06
    96/100 stars

    Images

    1) Product Images from "Partition Enrichment of Nucleotide Sequences (PINS) - A Generally Applicable, Sequence Based Method for Enrichment of Complex DNA Samples"

    Article Title: Partition Enrichment of Nucleotide Sequences (PINS) - A Generally Applicable, Sequence Based Method for Enrichment of Complex DNA Samples

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0106817

    Alignment of RGW sequence and HPV specific sequencing from this study to published sequences on human chromosome 8 and HPV18 sequences. Positions of the primers used for detection of the HPV18 target sequence in the enrichment (HPV5901f and HPV5994r) are illustrated by small triangles in HPU89349.
    Figure Legend Snippet: Alignment of RGW sequence and HPV specific sequencing from this study to published sequences on human chromosome 8 and HPV18 sequences. Positions of the primers used for detection of the HPV18 target sequence in the enrichment (HPV5901f and HPV5994r) are illustrated by small triangles in HPU89349.

    Techniques Used: Sequencing

    2) Product Images from "CRISPR/Cas9-based inactivation of human papillomavirus oncogenes E6 and E7 induces senescence in cervical cancer cells"

    Article Title: CRISPR/Cas9-based inactivation of human papillomavirus oncogenes E6 and E7 induces senescence in cervical cancer cells

    Journal: bioRxiv

    doi: 10.1101/2020.04.16.044446

    The senescent HeLa cells does not undergo apoptosis. (A) The senescent HeLa cells lysates knocked out for HPV18 E6 protein was analyzed by western blot. The blot was probed for apoptotic markers to detect PARP, cleaved-PARP, Caspase-3 and cleaved caspase-3 proteins. HeLa cells treated with staurosporine (Sts) at final concentrations of 0.4μM and 0.8μM was used as positive control for apoptosis. Representative image of three independent experiments is shown. (B) The cleaved PARP1 and cleaved caspase-3 band intensity from HPV18 E6 knockout cells were normalized with GAPDH and the mean band intensities were plotted versus the respective experiment. (C) The senescent HeLa cells lysates knocked out for HPV18 E7 protein was analyzed by western blot. The blot was probed in same way as explained in panel A. (D) The cleaved PARP1 and cleaved caspase-3 band intensity from HPV18 E7 knockout cells was normalized with GAPDH and the mean band intensities were plotted versus the respective experiment.
    Figure Legend Snippet: The senescent HeLa cells does not undergo apoptosis. (A) The senescent HeLa cells lysates knocked out for HPV18 E6 protein was analyzed by western blot. The blot was probed for apoptotic markers to detect PARP, cleaved-PARP, Caspase-3 and cleaved caspase-3 proteins. HeLa cells treated with staurosporine (Sts) at final concentrations of 0.4μM and 0.8μM was used as positive control for apoptosis. Representative image of three independent experiments is shown. (B) The cleaved PARP1 and cleaved caspase-3 band intensity from HPV18 E6 knockout cells were normalized with GAPDH and the mean band intensities were plotted versus the respective experiment. (C) The senescent HeLa cells lysates knocked out for HPV18 E7 protein was analyzed by western blot. The blot was probed in same way as explained in panel A. (D) The cleaved PARP1 and cleaved caspase-3 band intensity from HPV18 E7 knockout cells was normalized with GAPDH and the mean band intensities were plotted versus the respective experiment.

    Techniques Used: Western Blot, Positive Control, Knock-Out

    Re-introduction of HPV18 E6 in senescent HeLa cells. HPV18 E6 knock out senescent cells were transfected with either codon modified 18E6 expressing plasmid or empty plasmid followed by western blot analysis. The blot was probed for antibodies against p53, p21, 18E6 protein and actin. Representative image of two independent experiments is shown. (B) The mean p53 signal from two independent experiments was normalized with loading control actin and the relative intensity of p53 with Ctrl-1 was plotted versus the respective experiment.
    Figure Legend Snippet: Re-introduction of HPV18 E6 in senescent HeLa cells. HPV18 E6 knock out senescent cells were transfected with either codon modified 18E6 expressing plasmid or empty plasmid followed by western blot analysis. The blot was probed for antibodies against p53, p21, 18E6 protein and actin. Representative image of two independent experiments is shown. (B) The mean p53 signal from two independent experiments was normalized with loading control actin and the relative intensity of p53 with Ctrl-1 was plotted versus the respective experiment.

    Techniques Used: Knock-Out, Transfection, Modification, Expressing, Plasmid Preparation, Western Blot

    HPV18 E6 and E7 protein knockout as a result of Cas9 specific cleavage at predicted HPV genome sites. (A) Western blot analysis of puromycin selected HeLa cells expressing Cas9 (Ctrl-1), Cas9 with HPV18 E6 specific gRNAs (E6gRNA-1 and E6gRNA-2) and Cas9 with HPV16 E6 specific gRNA (Ctrl-2). The blot was probed for antibodies against HPV18 E6 and GAPDH. Representative image of four independent experiments is shown. (B) Quantification of HPV18 E6 signal intensity normalized with GAPDH and compared with Ctrl-1. (C) HeLa cells expressing Cas9 (Ctrl-1), Cas9 with HPV18 E7 specific gRNAs (E7gRNA-1 and E7gRNA-2) and Cas9 with HPV16 E7 specific gRNA (Ctrl-2) were analyzed by western blot. The blot was probed for HPV18 E7 and GAPDH antibodies. Representative image of four independent experiments is shown. (D) Quantification of HPV18 E7 signal intensity normalized with GAPDH and compared with Ctrl-1. Error bars represent mean values with standard deviation; * P
    Figure Legend Snippet: HPV18 E6 and E7 protein knockout as a result of Cas9 specific cleavage at predicted HPV genome sites. (A) Western blot analysis of puromycin selected HeLa cells expressing Cas9 (Ctrl-1), Cas9 with HPV18 E6 specific gRNAs (E6gRNA-1 and E6gRNA-2) and Cas9 with HPV16 E6 specific gRNA (Ctrl-2). The blot was probed for antibodies against HPV18 E6 and GAPDH. Representative image of four independent experiments is shown. (B) Quantification of HPV18 E6 signal intensity normalized with GAPDH and compared with Ctrl-1. (C) HeLa cells expressing Cas9 (Ctrl-1), Cas9 with HPV18 E7 specific gRNAs (E7gRNA-1 and E7gRNA-2) and Cas9 with HPV16 E7 specific gRNA (Ctrl-2) were analyzed by western blot. The blot was probed for HPV18 E7 and GAPDH antibodies. Representative image of four independent experiments is shown. (D) Quantification of HPV18 E7 signal intensity normalized with GAPDH and compared with Ctrl-1. Error bars represent mean values with standard deviation; * P

    Techniques Used: Knock-Out, Western Blot, Expressing, Standard Deviation

    HPV18 E6 and E7 inactivated HeLa cells display senescence phenotype. (A) Light microscopic images showing senescence –associated β-galactosidase assay of HeLa cells expressing Cas9 (Ctrl-1), Cas9 with 18E6 specific gRNAs (E6gRNA-1, E6gRNA-2) or 18E7 specific gRNAs (E7gRNA-1, E7gRNA-2) and Cas9 with 16E6 or 16E7 specific gRNA (Ctrl-2) treated HeLa cells. Scale bar 100μm. Representative image of three independent experiments is shown. (B) and (C) Indirect immunofluorescence microscopy of HeLa cells expressing Cas9 (Ctrl-1), Cas9 with 18E6 specific gRNAs (E6gRNA-1, E6gRNA-2) or 18E7 specific gRNAs (E7gRNA-1, E7gRNA-2), and Cas9 with nonspecific HPV16E6 and E7 gRNAs (Ctrl-2). Shown from left to right are DAPI stained nucleus, tubulin stained cytoplasm along with lamin B1 and merge of images. Representative image of three independent experiments is shown. Scale bar 20μm. (D) The cell surface area measurements of tubulin staining cells from panel B and C plotted for the respective experiment. Mean values from three independent experiments were shown. The Y-axis is in log 2 scale. (E) Quantitative measurements of cell nucleus surface area from DAPI stained cells from panel B. Mean values from three independent experiments were shown. The Y-axis is in log 2 scale. (E) The lamin B1 signal from HeLa cells in panel B and C were quantified from three independent experiments and mean values are shown from each experiment. The error bars indicate mean values with standard deviation. * P
    Figure Legend Snippet: HPV18 E6 and E7 inactivated HeLa cells display senescence phenotype. (A) Light microscopic images showing senescence –associated β-galactosidase assay of HeLa cells expressing Cas9 (Ctrl-1), Cas9 with 18E6 specific gRNAs (E6gRNA-1, E6gRNA-2) or 18E7 specific gRNAs (E7gRNA-1, E7gRNA-2) and Cas9 with 16E6 or 16E7 specific gRNA (Ctrl-2) treated HeLa cells. Scale bar 100μm. Representative image of three independent experiments is shown. (B) and (C) Indirect immunofluorescence microscopy of HeLa cells expressing Cas9 (Ctrl-1), Cas9 with 18E6 specific gRNAs (E6gRNA-1, E6gRNA-2) or 18E7 specific gRNAs (E7gRNA-1, E7gRNA-2), and Cas9 with nonspecific HPV16E6 and E7 gRNAs (Ctrl-2). Shown from left to right are DAPI stained nucleus, tubulin stained cytoplasm along with lamin B1 and merge of images. Representative image of three independent experiments is shown. Scale bar 20μm. (D) The cell surface area measurements of tubulin staining cells from panel B and C plotted for the respective experiment. Mean values from three independent experiments were shown. The Y-axis is in log 2 scale. (E) Quantitative measurements of cell nucleus surface area from DAPI stained cells from panel B. Mean values from three independent experiments were shown. The Y-axis is in log 2 scale. (E) The lamin B1 signal from HeLa cells in panel B and C were quantified from three independent experiments and mean values are shown from each experiment. The error bars indicate mean values with standard deviation. * P

    Techniques Used: Expressing, Immunofluorescence, Microscopy, Staining, Standard Deviation

    HPV18 E6 and E7 knockout restores p53 and pRb tumor suppressor pathways. (A) HeLa cells expressing only Cas9 (Ctrl-1), Cas9 with 18 E6 specific gRNAs (E6gRNA-1 and E6gRNA-2) and Cas9 with non-specific gRNA (Ctrl-2) were analyzed by western blot. The blot was probed to detect Flag-tagged Cas9 protein, p53, p21, HPV18 E6 and GAPDH. Representative image of three independent experiments is shown. Quantification of endogenous p53 and p21 levels were performed and relative intensities were plotted for the respective experiment in panel and (C). (D) Western blot analysis of protein lysates from cells treated similarly as explained in panel (A), but the gRNAs expressed were targeted for endogenous HPV18 E7 protein and the antibodies were against Flag-Cas9, retinoblastoma protein (pRb), p53, p21, and GAPDH. The lysates were re-run to detect phosphorylated-Rb (phosp-Rb). (E, F, G) The mean band intensities from three independent experiments were compared with those from Ctrl-1 and plotted versus the respective experiment. Relative intensities of (E) p53 (F) p21 (G) phosp-Rb were plotted. The error bars represent mean values with standard deviation. * P
    Figure Legend Snippet: HPV18 E6 and E7 knockout restores p53 and pRb tumor suppressor pathways. (A) HeLa cells expressing only Cas9 (Ctrl-1), Cas9 with 18 E6 specific gRNAs (E6gRNA-1 and E6gRNA-2) and Cas9 with non-specific gRNA (Ctrl-2) were analyzed by western blot. The blot was probed to detect Flag-tagged Cas9 protein, p53, p21, HPV18 E6 and GAPDH. Representative image of three independent experiments is shown. Quantification of endogenous p53 and p21 levels were performed and relative intensities were plotted for the respective experiment in panel and (C). (D) Western blot analysis of protein lysates from cells treated similarly as explained in panel (A), but the gRNAs expressed were targeted for endogenous HPV18 E7 protein and the antibodies were against Flag-Cas9, retinoblastoma protein (pRb), p53, p21, and GAPDH. The lysates were re-run to detect phosphorylated-Rb (phosp-Rb). (E, F, G) The mean band intensities from three independent experiments were compared with those from Ctrl-1 and plotted versus the respective experiment. Relative intensities of (E) p53 (F) p21 (G) phosp-Rb were plotted. The error bars represent mean values with standard deviation. * P

    Techniques Used: Knock-Out, Expressing, Western Blot, Standard Deviation

    HPV18 E6 and E7 inactivated cells are positive for senescence markers. Immunofluorescence microscopy of cells with DAPI stained nucleus, γ-H2Ax and α-tubulin stained cytoplasm after transfection with Cas9 (Ctrl-1), Cas9 with HPV18 E6 specific gRNAs (E6gRNA-1, E6gRNA-2) or E7 specific gRNAs (E7gRNA-1, E7gRNA-2) and Cas9 with nonspecific gRNAs (Ctrl-2). Panel (A) represents cells with HPV18 E6 gRNAs and controls. Panel (B) shows cells with HPV18 E7 specific gRNAs and control cells. White arrows indicate cytoplasmic chromatin fragments. Scale bar 20μm. Representative image of three independent experiments is shown in both panels A and B.
    Figure Legend Snippet: HPV18 E6 and E7 inactivated cells are positive for senescence markers. Immunofluorescence microscopy of cells with DAPI stained nucleus, γ-H2Ax and α-tubulin stained cytoplasm after transfection with Cas9 (Ctrl-1), Cas9 with HPV18 E6 specific gRNAs (E6gRNA-1, E6gRNA-2) or E7 specific gRNAs (E7gRNA-1, E7gRNA-2) and Cas9 with nonspecific gRNAs (Ctrl-2). Panel (A) represents cells with HPV18 E6 gRNAs and controls. Panel (B) shows cells with HPV18 E7 specific gRNAs and control cells. White arrows indicate cytoplasmic chromatin fragments. Scale bar 20μm. Representative image of three independent experiments is shown in both panels A and B.

    Techniques Used: Immunofluorescence, Microscopy, Staining, Transfection

    Related Articles

    Methylation:

    Article Title: Electrochemical affinity biosensors for fast detection of gene-specific methylations with no need for bisulfite and amplification treatments
    Article Snippet: A Salivette® collection device (Sarstedt) was used for collecting the saliva samples. .. Fragmented HeLa genomic DNA used as control in the cells experiments was a component of the EpiMark Methylated DNA Enrichment Kit (New England Biolabs, Inc.). ..

    Article Title: Aberrant Promoter Hypermethylation of RASSF1a and BRCA1 in Circulating Cell-Free Tumor DNA Serves as a Biomarker of Ovarian Carcinoma
    Article Snippet: Promoter hypermethylation was assessed performing Methylation specific PCR using specific primers adapted from Honorio S et al., (2003) and Esteller M et al., (2000) (RASSF1A Methylated forward -5’-GGGTTTTGCGAGAGCGCGT-3’, RASSF1A Methylated reverse -5’-GCTAACAAACGCGAACCG-3’, RASSF1A Unmethylated forward-5’- GGTTTTGTGAGAGTGTGTTTAGT-3’, RASSF1A Unmethylated reverse- 5’CACTAACAAACACAAACCAAACA-3’; BRCA1 Methylated forward-5’-GGGTTTTGCGAGAGCGCGT-3’, BRCA1 Methylated reverse-5’ -AAAACTCAACGAACTCACGCCG-3’, BRCA1 Unmethylated forward-5’- TTGGTTTTTGTGGTAATGGAAAAGTGT-3’, BRCA1 Unmethylated reverse-5’-CAAAAAATCTCAACAAACTCACACCA-3’). .. Universally Methylated HeLa Genomic DNA (New England Biolabs Inc, England) was used as a positive control for the methylated allele. .. Peripheral blood-derived DNA from healthy non-cancer patients was used as a control for unmethylated allele and no template control served as a negative control.10µl of the PCR product was loaded on to a 2.5% Agarose gel and visualized by staining with Ethidium Bromide.

    Article Title: Evaluating the Global CpG Methylation Status of Native DNA Utilizing a Bipartite Split-Luciferase Sensor
    Article Snippet: All designed DNA targets were annealed in 1x BamHI Buffer (NEB) using the following procedure: heating to 95 °C for 7 min, cooling to 56 °C at a rate of 0.1 °C sec−1 , equilibrating at 56 °C for 5 min, cooling to 25 °C at a rate of 0.1 °C sec−1 and finally equilibrating at 25 °C for 10 min using a Labnet Multi Gene II thermocycler. .. The pETDuet recombinant plasmid variant, pET (7667 bp), and purified HeLa genomic DNA were methylated using M. SssI CpG methyltransferase (NEB) according to the manufacturer’s protocol. .. The extent of protection was determined by using the methylation sensitive endonucleases BstUI, where the cleavage of the target sequence is blocked by CpG methylation and McrBC, where the cleavage of the target sequence requires either symmetrical methylation or hemimethylation.

    Positive Control:

    Article Title: Aberrant Promoter Hypermethylation of RASSF1a and BRCA1 in Circulating Cell-Free Tumor DNA Serves as a Biomarker of Ovarian Carcinoma
    Article Snippet: Promoter hypermethylation was assessed performing Methylation specific PCR using specific primers adapted from Honorio S et al., (2003) and Esteller M et al., (2000) (RASSF1A Methylated forward -5’-GGGTTTTGCGAGAGCGCGT-3’, RASSF1A Methylated reverse -5’-GCTAACAAACGCGAACCG-3’, RASSF1A Unmethylated forward-5’- GGTTTTGTGAGAGTGTGTTTAGT-3’, RASSF1A Unmethylated reverse- 5’CACTAACAAACACAAACCAAACA-3’; BRCA1 Methylated forward-5’-GGGTTTTGCGAGAGCGCGT-3’, BRCA1 Methylated reverse-5’ -AAAACTCAACGAACTCACGCCG-3’, BRCA1 Unmethylated forward-5’- TTGGTTTTTGTGGTAATGGAAAAGTGT-3’, BRCA1 Unmethylated reverse-5’-CAAAAAATCTCAACAAACTCACACCA-3’). .. Universally Methylated HeLa Genomic DNA (New England Biolabs Inc, England) was used as a positive control for the methylated allele. .. Peripheral blood-derived DNA from healthy non-cancer patients was used as a control for unmethylated allele and no template control served as a negative control.10µl of the PCR product was loaded on to a 2.5% Agarose gel and visualized by staining with Ethidium Bromide.

    Concentration Assay:

    Article Title: Partition Enrichment of Nucleotide Sequences (PINS) - A Generally Applicable, Sequence Based Method for Enrichment of Complex DNA Samples
    Article Snippet: .. Nucleic acid templates HeLa DNA containing HPV18 was purchased from New England Biolabs in a concentration of 100 ng/µL (NEB-N4006S). .. Background DNA was extracted from leukocytes from an HPV18 negative blood sample and DNA was extracted using a DNA Extraction kit (Fermentas – GeneJETTM Genomic DNA Purification Kit).

    Recombinant:

    Article Title: Evaluating the Global CpG Methylation Status of Native DNA Utilizing a Bipartite Split-Luciferase Sensor
    Article Snippet: All designed DNA targets were annealed in 1x BamHI Buffer (NEB) using the following procedure: heating to 95 °C for 7 min, cooling to 56 °C at a rate of 0.1 °C sec−1 , equilibrating at 56 °C for 5 min, cooling to 25 °C at a rate of 0.1 °C sec−1 and finally equilibrating at 25 °C for 10 min using a Labnet Multi Gene II thermocycler. .. The pETDuet recombinant plasmid variant, pET (7667 bp), and purified HeLa genomic DNA were methylated using M. SssI CpG methyltransferase (NEB) according to the manufacturer’s protocol. .. The extent of protection was determined by using the methylation sensitive endonucleases BstUI, where the cleavage of the target sequence is blocked by CpG methylation and McrBC, where the cleavage of the target sequence requires either symmetrical methylation or hemimethylation.

    Plasmid Preparation:

    Article Title: Evaluating the Global CpG Methylation Status of Native DNA Utilizing a Bipartite Split-Luciferase Sensor
    Article Snippet: All designed DNA targets were annealed in 1x BamHI Buffer (NEB) using the following procedure: heating to 95 °C for 7 min, cooling to 56 °C at a rate of 0.1 °C sec−1 , equilibrating at 56 °C for 5 min, cooling to 25 °C at a rate of 0.1 °C sec−1 and finally equilibrating at 25 °C for 10 min using a Labnet Multi Gene II thermocycler. .. The pETDuet recombinant plasmid variant, pET (7667 bp), and purified HeLa genomic DNA were methylated using M. SssI CpG methyltransferase (NEB) according to the manufacturer’s protocol. .. The extent of protection was determined by using the methylation sensitive endonucleases BstUI, where the cleavage of the target sequence is blocked by CpG methylation and McrBC, where the cleavage of the target sequence requires either symmetrical methylation or hemimethylation.

    Variant Assay:

    Article Title: Evaluating the Global CpG Methylation Status of Native DNA Utilizing a Bipartite Split-Luciferase Sensor
    Article Snippet: All designed DNA targets were annealed in 1x BamHI Buffer (NEB) using the following procedure: heating to 95 °C for 7 min, cooling to 56 °C at a rate of 0.1 °C sec−1 , equilibrating at 56 °C for 5 min, cooling to 25 °C at a rate of 0.1 °C sec−1 and finally equilibrating at 25 °C for 10 min using a Labnet Multi Gene II thermocycler. .. The pETDuet recombinant plasmid variant, pET (7667 bp), and purified HeLa genomic DNA were methylated using M. SssI CpG methyltransferase (NEB) according to the manufacturer’s protocol. .. The extent of protection was determined by using the methylation sensitive endonucleases BstUI, where the cleavage of the target sequence is blocked by CpG methylation and McrBC, where the cleavage of the target sequence requires either symmetrical methylation or hemimethylation.

    Positron Emission Tomography:

    Article Title: Evaluating the Global CpG Methylation Status of Native DNA Utilizing a Bipartite Split-Luciferase Sensor
    Article Snippet: All designed DNA targets were annealed in 1x BamHI Buffer (NEB) using the following procedure: heating to 95 °C for 7 min, cooling to 56 °C at a rate of 0.1 °C sec−1 , equilibrating at 56 °C for 5 min, cooling to 25 °C at a rate of 0.1 °C sec−1 and finally equilibrating at 25 °C for 10 min using a Labnet Multi Gene II thermocycler. .. The pETDuet recombinant plasmid variant, pET (7667 bp), and purified HeLa genomic DNA were methylated using M. SssI CpG methyltransferase (NEB) according to the manufacturer’s protocol. .. The extent of protection was determined by using the methylation sensitive endonucleases BstUI, where the cleavage of the target sequence is blocked by CpG methylation and McrBC, where the cleavage of the target sequence requires either symmetrical methylation or hemimethylation.

    Purification:

    Article Title: Evaluating the Global CpG Methylation Status of Native DNA Utilizing a Bipartite Split-Luciferase Sensor
    Article Snippet: All designed DNA targets were annealed in 1x BamHI Buffer (NEB) using the following procedure: heating to 95 °C for 7 min, cooling to 56 °C at a rate of 0.1 °C sec−1 , equilibrating at 56 °C for 5 min, cooling to 25 °C at a rate of 0.1 °C sec−1 and finally equilibrating at 25 °C for 10 min using a Labnet Multi Gene II thermocycler. .. The pETDuet recombinant plasmid variant, pET (7667 bp), and purified HeLa genomic DNA were methylated using M. SssI CpG methyltransferase (NEB) according to the manufacturer’s protocol. .. The extent of protection was determined by using the methylation sensitive endonucleases BstUI, where the cleavage of the target sequence is blocked by CpG methylation and McrBC, where the cleavage of the target sequence requires either symmetrical methylation or hemimethylation.

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    New England Biolabs hpv18
    Alignment of RGW sequence and HPV specific sequencing from this study to published sequences on human chromosome 8 and <t>HPV18</t> sequences. Positions of the primers used for detection of the HPV18 target sequence in the enrichment (HPV5901f and HPV5994r) are illustrated by small triangles in HPU89349.
    Hpv18, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hpv18/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hpv18 - by Bioz Stars, 2021-06
    96/100 stars
      Buy from Supplier

    99
    New England Biolabs maltose binding protein mbp hpv18 e1 e4 fusion proteins
    Alignment of RGW sequence and HPV specific sequencing from this study to published sequences on human chromosome 8 and <t>HPV18</t> sequences. Positions of the primers used for detection of the HPV18 target sequence in the enrichment (HPV5901f and HPV5994r) are illustrated by small triangles in HPU89349.
    Maltose Binding Protein Mbp Hpv18 E1 E4 Fusion Proteins, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/maltose binding protein mbp hpv18 e1 e4 fusion proteins/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    maltose binding protein mbp hpv18 e1 e4 fusion proteins - by Bioz Stars, 2021-06
    99/100 stars
      Buy from Supplier

    Image Search Results


    Alignment of RGW sequence and HPV specific sequencing from this study to published sequences on human chromosome 8 and HPV18 sequences. Positions of the primers used for detection of the HPV18 target sequence in the enrichment (HPV5901f and HPV5994r) are illustrated by small triangles in HPU89349.

    Journal: PLoS ONE

    Article Title: Partition Enrichment of Nucleotide Sequences (PINS) - A Generally Applicable, Sequence Based Method for Enrichment of Complex DNA Samples

    doi: 10.1371/journal.pone.0106817

    Figure Lengend Snippet: Alignment of RGW sequence and HPV specific sequencing from this study to published sequences on human chromosome 8 and HPV18 sequences. Positions of the primers used for detection of the HPV18 target sequence in the enrichment (HPV5901f and HPV5994r) are illustrated by small triangles in HPU89349.

    Article Snippet: Nucleic acid templates HeLa DNA containing HPV18 was purchased from New England Biolabs in a concentration of 100 ng/µL (NEB-N4006S).

    Techniques: Sequencing