Structured Review

Merck KGaA hptlc plates
F1394 increases free cholesterol/cholesterol ester ratio in LDs. <t>Huh7</t> cells were cultured for 24 h in the absence (Control) or presence of 10 μg/ml F1394 or 10 μg/ml 58-035 or treated with MβCD/cholesterol in LPDS (Chol) or MβCD/cholesterol plus 58-035 in LPDS (Chol + 58-035) as described in Materials and Methods . LD fractions were prepared and lipids analyzed by <t>HPTLC</t> as described in Materials and Methods . Results indicate the mean ± SD ( n = 3). * p = 0.05, ** p
Hptlc Plates, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hptlc plates - by Bioz Stars, 2020-09
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1) Product Images from "Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes"

Article Title: Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes

Journal: Molecular Biology of the Cell

doi: 10.1091/mbc.E15-10-0730

F1394 increases free cholesterol/cholesterol ester ratio in LDs. Huh7 cells were cultured for 24 h in the absence (Control) or presence of 10 μg/ml F1394 or 10 μg/ml 58-035 or treated with MβCD/cholesterol in LPDS (Chol) or MβCD/cholesterol plus 58-035 in LPDS (Chol + 58-035) as described in Materials and Methods . LD fractions were prepared and lipids analyzed by HPTLC as described in Materials and Methods . Results indicate the mean ± SD ( n = 3). * p = 0.05, ** p
Figure Legend Snippet: F1394 increases free cholesterol/cholesterol ester ratio in LDs. Huh7 cells were cultured for 24 h in the absence (Control) or presence of 10 μg/ml F1394 or 10 μg/ml 58-035 or treated with MβCD/cholesterol in LPDS (Chol) or MβCD/cholesterol plus 58-035 in LPDS (Chol + 58-035) as described in Materials and Methods . LD fractions were prepared and lipids analyzed by HPTLC as described in Materials and Methods . Results indicate the mean ± SD ( n = 3). * p = 0.05, ** p

Techniques Used: Cell Culture, High Performance Thin Layer Chromatography

Related Articles

Enzyme-linked Immunosorbent Assay:

Article Title: Ganglioside Structure Dictates Signal Transduction by Cholera Toxin and Association with Caveolae-like Membrane Domains in Polarized Epithelia
Article Snippet: .. The fractions were separated on HPTLC plates (Silica Gel 60; E. Merck, Darmstadt, Federal Republic of Germany), and the gangliotetrose series (GM1, GD1a, GD1b, and GT1b) was detected by ligand blotting with CT B subunit (1 nM) and anti–CT B subunit antibodies in an ELISA performed on the HPTLC plate. ..

Isolation:

Article Title: Effects of Insulin and High Glucose on Human Meibomian Gland Epithelial Cells
Article Snippet: .. Total cellular lipids were extracted from samples containing equivalent amount of cells and isolated on HPTLC plates (Silica Gel 60; Merck, Darmstadt, Germany), as previously described. .. Cells were lysed in SDS Laemmli buffer (Bio-Rad, Hercules, CA, USA) supplemented with 1% protease inhibitor cocktail, 200 uM sodium orthovanadate and 5% β-mercaptoethanol (all from Sigma-Aldrich Corp., St. Louis, MO, USA).

High Performance Thin Layer Chromatography:

Article Title: Effects of Insulin and High Glucose on Human Meibomian Gland Epithelial Cells
Article Snippet: .. Total cellular lipids were extracted from samples containing equivalent amount of cells and isolated on HPTLC plates (Silica Gel 60; Merck, Darmstadt, Germany), as previously described. .. Cells were lysed in SDS Laemmli buffer (Bio-Rad, Hercules, CA, USA) supplemented with 1% protease inhibitor cocktail, 200 uM sodium orthovanadate and 5% β-mercaptoethanol (all from Sigma-Aldrich Corp., St. Louis, MO, USA).

Article Title: Stability-Indicating HPTLC Method for Studying Stress Degradation Behavior of Sulbutiamine HCl
Article Snippet: .. HPTLC plates (20 × 10 cm) coated with 60 F254 silica gel (Merck, Darmstadt, Germany) with 0.2 mm thickness were used as a stationary phase. .. During TLC scanning, the scanning mode was absorbance, source of radiation was deuterium lamp.

Article Title: Ganglioside Structure Dictates Signal Transduction by Cholera Toxin and Association with Caveolae-like Membrane Domains in Polarized Epithelia
Article Snippet: .. The fractions were separated on HPTLC plates (Silica Gel 60; E. Merck, Darmstadt, Federal Republic of Germany), and the gangliotetrose series (GM1, GD1a, GD1b, and GT1b) was detected by ligand blotting with CT B subunit (1 nM) and anti–CT B subunit antibodies in an ELISA performed on the HPTLC plate. ..

Article Title: Differentiation-related changes in lipid classes with long-chain and very long-chain polyenoic fatty acids in rat spermatogenic cells [S]
Article Snippet: .. For phospholipid class composition, aliquots of the extracts were spotted on HPTLC plates (Merck, Germany), along with commercial standards, and resolved with chloroform:methanol:acetic acid:water (50:37.5:3.5:2 by vol) ( ). ..

Article Title: Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes
Article Snippet: .. Thin-layer chromatography Lipids were extracted ( ) from Huh7 cells and applied to HPTLC plates (Merck, Darmstadt, Germany). ..

Article Title: Biochemical studies on sphingolipids of Artemia franciscana: novel neutral glycosphingolipids [S]
Article Snippet: .. In Iatrobeads column chromatography, GSLs on HPTLC plates of silica gel 60 (Merck KGaA) were visualized by spraying with orcinol-sulfuric acid reagent followed by heating at 110°C. .. For determination of the composition of fatty acids and sugars, 0.1–0.2 mg of each GSL was methanolyzed with 1 M anhydrous methanolic HCl at 100°C for 3 h. The produced fatty acid methyl esters were extracted with n -hexane and analyzed by GC and GC-MS.

Article Title: Incorporation of branched-chain fatty acid into cellular lipids and caspase-independent apoptosis in human breast cancer cell line, SKBR-3
Article Snippet: .. Lipids partitioned into chloroform layer were concentrated and spotted onto HPTLC plates (Merck, Darmstadt, Germany) for a separation of triacylglycerol, (TG) and phospholipid (PL) fractions as described previously [ ]. .. In the case of separation of phospholipids, the HPTLC plates were developed stepwise with chloroform/acetone/methanol (9:0.5:0.5, by vol.), chloroform/ethyl acetate/methanol/2-propanol/triethylamine/water (6.4: 1.5: 1.7: 0.05: 0.3: 0.2, by vol.).

Article Title: Comprehensive quantification of ceramide species in human stratum corneum [S]
Article Snippet: .. Briefly, HPTLC-plates (silica gel 60 with concentrating zone, 20 cm × 10 cm; Merck, Darmstadt, Germany) were used for the separation. ..

Thin Layer Chromatography:

Article Title: Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes
Article Snippet: .. Thin-layer chromatography Lipids were extracted ( ) from Huh7 cells and applied to HPTLC plates (Merck, Darmstadt, Germany). ..

Column Chromatography:

Article Title: Biochemical studies on sphingolipids of Artemia franciscana: novel neutral glycosphingolipids [S]
Article Snippet: .. In Iatrobeads column chromatography, GSLs on HPTLC plates of silica gel 60 (Merck KGaA) were visualized by spraying with orcinol-sulfuric acid reagent followed by heating at 110°C. .. For determination of the composition of fatty acids and sugars, 0.1–0.2 mg of each GSL was methanolyzed with 1 M anhydrous methanolic HCl at 100°C for 3 h. The produced fatty acid methyl esters were extracted with n -hexane and analyzed by GC and GC-MS.

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  • 89
    Merck KGaA pre coated rp hptlc silica gel 60 rp 18 f254 s aluminium plates
    Reverse phase <t>HPTLC</t> chromatograms and visualization. 3D overlay chromatograms of Ghee (Track 1), Ghrita (Track2), Fraction A (Track 3) and Fraction B (Track 4) and visualization of the developed silica <t>gel</t> 60 <t>RP-18</t> F 254 s aluminium plates observed under UV light @254 and @366 nm showing Ghee (Track 1), Ghrita (Track2), Fraction A (Track 3) and Fraction B (Track 4) respectively.
    Pre Coated Rp Hptlc Silica Gel 60 Rp 18 F254 S Aluminium Plates, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 89/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pre coated rp hptlc silica gel 60 rp 18 f254 s aluminium plates/product/Merck KGaA
    Average 89 stars, based on 7 article reviews
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    91
    Merck KGaA hptlc plates silica gel 60 cn
    Comparison of <t>HPTLC-ESI-MS</t> spectra for E 133, E 127, and E 111 obtained from HPTLC foils silica <t>gel</t> 60 ( A ) with UTLC-Vis-ESI-MS spectra recorded from electrospun PAN nanofiber phases ( B ); chronogram of elution profiles ( C ) and mass spectra of E 105 and PAN phase background ( D ); scheme of the elution head ( E ) and some fiber mat imprints after elution on the electrospun PAN nanofiber phase ( F ).
    Hptlc Plates Silica Gel 60 Cn, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hptlc plates silica gel 60 cn/product/Merck KGaA
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    93
    Merck KGaA hptlc rp 18w plates
    Migration distance of phenolic acids vs. butanol concentration in the mobile phase for <t>HPTLC</t> (a) and PPEC (b) experiments. The mobile phase: butanol (0.89–3.27 M; 8.14–30.00% v/v), 10% acetonitrile, 0.1144 M SDS, and aqueous buffer of pH 7.0 (0.88 mM of citric acid and 8.23 mM of disodium hydrogen phosphate). The stationary phase: HPTLC <t>RP-18W.</t> TLC experiment time: 20 min. PPEC: polarization voltage 800 V; experiment time 8 min.
    Hptlc Rp 18w Plates, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Merck KGaA pre coated hptlc plates
    Analysis of <t>L-DOPA.</t> (a) <t>HPTLC.</t> 1. Standard tyrosine. 2. Standard L-DOPA. 3. Biotransformation product. (b) Analysis of L-DOPA using HPLC. 1. Standard L-DOPA. 2. Biotransformation product.
    Pre Coated Hptlc Plates, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pre coated hptlc plates/product/Merck KGaA
    Average 88 stars, based on 9 article reviews
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    pre coated hptlc plates - by Bioz Stars, 2020-09
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    Image Search Results


    Reverse phase HPTLC chromatograms and visualization. 3D overlay chromatograms of Ghee (Track 1), Ghrita (Track2), Fraction A (Track 3) and Fraction B (Track 4) and visualization of the developed silica gel 60 RP-18 F 254 s aluminium plates observed under UV light @254 and @366 nm showing Ghee (Track 1), Ghrita (Track2), Fraction A (Track 3) and Fraction B (Track 4) respectively.

    Journal: BMC Complementary and Alternative Medicine

    Article Title: Reverse engineering of Ayurvedic lipid based formulation, ghrita by combined column chromatography, normal and reverse phase HPTLC analysis

    doi: 10.1186/s12906-015-0568-9

    Figure Lengend Snippet: Reverse phase HPTLC chromatograms and visualization. 3D overlay chromatograms of Ghee (Track 1), Ghrita (Track2), Fraction A (Track 3) and Fraction B (Track 4) and visualization of the developed silica gel 60 RP-18 F 254 s aluminium plates observed under UV light @254 and @366 nm showing Ghee (Track 1), Ghrita (Track2), Fraction A (Track 3) and Fraction B (Track 4) respectively.

    Article Snippet: Pre-coated silica gel GF254 aluminium plates for normal phase and the pre-coated RP-HPTLC silica gel 60 RP-18 F254 s aluminium plates for reverse phase chromatography were purchased from Merck, India to carry out HPTLC analysis on CAMAG HPTLC system, Switzerland.

    Techniques: High Performance Thin Layer Chromatography

    Comparison of HPTLC-ESI-MS spectra for E 133, E 127, and E 111 obtained from HPTLC foils silica gel 60 ( A ) with UTLC-Vis-ESI-MS spectra recorded from electrospun PAN nanofiber phases ( B ); chronogram of elution profiles ( C ) and mass spectra of E 105 and PAN phase background ( D ); scheme of the elution head ( E ) and some fiber mat imprints after elution on the electrospun PAN nanofiber phase ( F ).

    Journal: Nanomaterials

    Article Title: Performance of Electropun Polyacrylonitrile Nanofibrous Phases, Shown for the Separation of Water-Soluble Food Dyes via UTLC-Vis-ESI-MS

    doi: 10.3390/nano7080218

    Figure Lengend Snippet: Comparison of HPTLC-ESI-MS spectra for E 133, E 127, and E 111 obtained from HPTLC foils silica gel 60 ( A ) with UTLC-Vis-ESI-MS spectra recorded from electrospun PAN nanofiber phases ( B ); chronogram of elution profiles ( C ) and mass spectra of E 105 and PAN phase background ( D ); scheme of the elution head ( E ) and some fiber mat imprints after elution on the electrospun PAN nanofiber phase ( F ).

    Article Snippet: Toluene (purity ≥ 99.8%) and HPTLC silica gel 60 aluminum foils and HPTLC plates silica gel 60 CN (cyano phase) were purchased from Merck, Darmstadt, Germany.

    Techniques: High Performance Thin Layer Chromatography, Mass Spectrometry

    Comparison of mean mobile phase velocities with standard deviation (SD, n = 3) on HPTLC foil silica gel 60 ( ), HPTLC plate silica gel CN ( ) and electrospun PAN nanofiber phase ( ).

    Journal: Nanomaterials

    Article Title: Performance of Electropun Polyacrylonitrile Nanofibrous Phases, Shown for the Separation of Water-Soluble Food Dyes via UTLC-Vis-ESI-MS

    doi: 10.3390/nano7080218

    Figure Lengend Snippet: Comparison of mean mobile phase velocities with standard deviation (SD, n = 3) on HPTLC foil silica gel 60 ( ), HPTLC plate silica gel CN ( ) and electrospun PAN nanofiber phase ( ).

    Article Snippet: Toluene (purity ≥ 99.8%) and HPTLC silica gel 60 aluminum foils and HPTLC plates silica gel 60 CN (cyano phase) were purchased from Merck, Darmstadt, Germany.

    Techniques: Standard Deviation, High Performance Thin Layer Chromatography

    Migration distance of phenolic acids vs. butanol concentration in the mobile phase for HPTLC (a) and PPEC (b) experiments. The mobile phase: butanol (0.89–3.27 M; 8.14–30.00% v/v), 10% acetonitrile, 0.1144 M SDS, and aqueous buffer of pH 7.0 (0.88 mM of citric acid and 8.23 mM of disodium hydrogen phosphate). The stationary phase: HPTLC RP-18W. TLC experiment time: 20 min. PPEC: polarization voltage 800 V; experiment time 8 min.

    Journal: Journal of Analytical Methods in Chemistry

    Article Title: Comparison of Phenolic Compound Separations by HPTLC and PPEC with SDS as the Mobile Phase Component

    doi: 10.1155/2019/6845340

    Figure Lengend Snippet: Migration distance of phenolic acids vs. butanol concentration in the mobile phase for HPTLC (a) and PPEC (b) experiments. The mobile phase: butanol (0.89–3.27 M; 8.14–30.00% v/v), 10% acetonitrile, 0.1144 M SDS, and aqueous buffer of pH 7.0 (0.88 mM of citric acid and 8.23 mM of disodium hydrogen phosphate). The stationary phase: HPTLC RP-18W. TLC experiment time: 20 min. PPEC: polarization voltage 800 V; experiment time 8 min.

    Article Snippet: HPTLC RP-18W plates of size 10 cm × 10 cm (Merck, Darmstadt, Germany) were used in the experiments.

    Techniques: Migration, Concentration Assay, High Performance Thin Layer Chromatography, Thin Layer Chromatography

    Migration distance of phenolic acids vs. SDS concentration in the mobile phase for HPTLC (a) and PPEC (b) experiments. The mobile phase: butanol (0.89 M; 8.14% v/v), 10% acetonitrile, and SDS in the range of 0.0143 to 0.1144 M aqueous buffer of pH 7.0 (0.88 mM of citric acid and 8.23 mM of disodium hydrogen phosphate). The stationary phase: HPTLC RP-18W. TLC: average experiment time 20 min. PPEC: polarization voltage 800 V; experiment time 8 min.

    Journal: Journal of Analytical Methods in Chemistry

    Article Title: Comparison of Phenolic Compound Separations by HPTLC and PPEC with SDS as the Mobile Phase Component

    doi: 10.1155/2019/6845340

    Figure Lengend Snippet: Migration distance of phenolic acids vs. SDS concentration in the mobile phase for HPTLC (a) and PPEC (b) experiments. The mobile phase: butanol (0.89 M; 8.14% v/v), 10% acetonitrile, and SDS in the range of 0.0143 to 0.1144 M aqueous buffer of pH 7.0 (0.88 mM of citric acid and 8.23 mM of disodium hydrogen phosphate). The stationary phase: HPTLC RP-18W. TLC: average experiment time 20 min. PPEC: polarization voltage 800 V; experiment time 8 min.

    Article Snippet: HPTLC RP-18W plates of size 10 cm × 10 cm (Merck, Darmstadt, Germany) were used in the experiments.

    Techniques: Migration, Concentration Assay, High Performance Thin Layer Chromatography, Thin Layer Chromatography

    Migration distance of flavonoids vs. SDS concentration in the mobile phase for HPTLC (a) and PPEC (b) experiments. The mobile phase: butanol (0.89 M; 8.14% v/v), 10% acetonitrile, and SDS in the range of 0.0143 to 0.1144 M aqueous buffer of pH 7.0 (0.88 mM of citric acid and 8.23 mM of disodium hydrogen phosphate). The stationary phase: HPTLC RP-18W. TLC: experiment time 20 min. PPEC: polarization voltage 800 V; experiment time 8 min.

    Journal: Journal of Analytical Methods in Chemistry

    Article Title: Comparison of Phenolic Compound Separations by HPTLC and PPEC with SDS as the Mobile Phase Component

    doi: 10.1155/2019/6845340

    Figure Lengend Snippet: Migration distance of flavonoids vs. SDS concentration in the mobile phase for HPTLC (a) and PPEC (b) experiments. The mobile phase: butanol (0.89 M; 8.14% v/v), 10% acetonitrile, and SDS in the range of 0.0143 to 0.1144 M aqueous buffer of pH 7.0 (0.88 mM of citric acid and 8.23 mM of disodium hydrogen phosphate). The stationary phase: HPTLC RP-18W. TLC: experiment time 20 min. PPEC: polarization voltage 800 V; experiment time 8 min.

    Article Snippet: HPTLC RP-18W plates of size 10 cm × 10 cm (Merck, Darmstadt, Germany) were used in the experiments.

    Techniques: Migration, Concentration Assay, High Performance Thin Layer Chromatography, Thin Layer Chromatography

    Migration distance of flavonoids vs. butanol concentration in the mobile phase for HPTLC (a) and PPEC (b) experiments. The mobile phase: butanol (0.89–3.27 M; 8.14–30.00% v/v), 10% acetonitrile, 0.1144 M SDS, and aqueous buffer of pH 7.0 (0.88 mM of citric acid and 8.23 mM of disodium hydrogen phosphate). The stationary phase: HPTLC RP-18W. TLC: experiment time 20 min. PPEC: polarization voltage 800 V; experiment time 8 min.

    Journal: Journal of Analytical Methods in Chemistry

    Article Title: Comparison of Phenolic Compound Separations by HPTLC and PPEC with SDS as the Mobile Phase Component

    doi: 10.1155/2019/6845340

    Figure Lengend Snippet: Migration distance of flavonoids vs. butanol concentration in the mobile phase for HPTLC (a) and PPEC (b) experiments. The mobile phase: butanol (0.89–3.27 M; 8.14–30.00% v/v), 10% acetonitrile, 0.1144 M SDS, and aqueous buffer of pH 7.0 (0.88 mM of citric acid and 8.23 mM of disodium hydrogen phosphate). The stationary phase: HPTLC RP-18W. TLC: experiment time 20 min. PPEC: polarization voltage 800 V; experiment time 8 min.

    Article Snippet: HPTLC RP-18W plates of size 10 cm × 10 cm (Merck, Darmstadt, Germany) were used in the experiments.

    Techniques: Migration, Concentration Assay, High Performance Thin Layer Chromatography, Thin Layer Chromatography

    Separation of the mixture of flavonoids and phenolic acids with the use of HPTLC (a) and PPEC (b) techniques. Experiment conditions: the mobile phase: butanol 1.2 M, acetonitrile 10% v/v, aqueous buffer of pH 7.0 (0.88 mM of citric acid and 8.23 mM of disodium hydrogen phosphate), and 0.040 M of SDS; the stationary phase: HPTLC RP-18W. PPEC: polarization voltage 1400 V; experiment time 8 min; experiment temperature 38 o C. The order of solutes for HPTLC: (1) rutin, (2) hesperidin; (3) trans -cinnamic acid and hesperidin; (4) caffeic acid and o -coumaric acid; (5) flavanone; (6) vanillic acid. The order of compounds in the PPEC experiment: (1) flavanone; (2) trans -cinnamic acid and hesperetin; (3) o -coumaric acid; (4) rutin; (5) hesperidin; (6) vanillic acid; (7) caffeic acid.

    Journal: Journal of Analytical Methods in Chemistry

    Article Title: Comparison of Phenolic Compound Separations by HPTLC and PPEC with SDS as the Mobile Phase Component

    doi: 10.1155/2019/6845340

    Figure Lengend Snippet: Separation of the mixture of flavonoids and phenolic acids with the use of HPTLC (a) and PPEC (b) techniques. Experiment conditions: the mobile phase: butanol 1.2 M, acetonitrile 10% v/v, aqueous buffer of pH 7.0 (0.88 mM of citric acid and 8.23 mM of disodium hydrogen phosphate), and 0.040 M of SDS; the stationary phase: HPTLC RP-18W. PPEC: polarization voltage 1400 V; experiment time 8 min; experiment temperature 38 o C. The order of solutes for HPTLC: (1) rutin, (2) hesperidin; (3) trans -cinnamic acid and hesperidin; (4) caffeic acid and o -coumaric acid; (5) flavanone; (6) vanillic acid. The order of compounds in the PPEC experiment: (1) flavanone; (2) trans -cinnamic acid and hesperetin; (3) o -coumaric acid; (4) rutin; (5) hesperidin; (6) vanillic acid; (7) caffeic acid.

    Article Snippet: HPTLC RP-18W plates of size 10 cm × 10 cm (Merck, Darmstadt, Germany) were used in the experiments.

    Techniques: High Performance Thin Layer Chromatography

    Migration distance of phenolic acids vs. mobile phase buffer pH in the mobile phase for HPTLC (a) and PPEC (b) experiments. The mobile phase: butanol (0.89 M; 8.14% v/v), 10% acetonitrile, 0.1144 mM SDS, and aqueous buffer (citric acid, tris(hydroxymethyl)aminomethane, glycine 0.8 mM titrated to proper pH with KOH or HCl). The stationary phase: HPTLC RP-18W. TLC: experiment time 20 min. PPEC: polarization voltage 800 V; experiment time 8 min.

    Journal: Journal of Analytical Methods in Chemistry

    Article Title: Comparison of Phenolic Compound Separations by HPTLC and PPEC with SDS as the Mobile Phase Component

    doi: 10.1155/2019/6845340

    Figure Lengend Snippet: Migration distance of phenolic acids vs. mobile phase buffer pH in the mobile phase for HPTLC (a) and PPEC (b) experiments. The mobile phase: butanol (0.89 M; 8.14% v/v), 10% acetonitrile, 0.1144 mM SDS, and aqueous buffer (citric acid, tris(hydroxymethyl)aminomethane, glycine 0.8 mM titrated to proper pH with KOH or HCl). The stationary phase: HPTLC RP-18W. TLC: experiment time 20 min. PPEC: polarization voltage 800 V; experiment time 8 min.

    Article Snippet: HPTLC RP-18W plates of size 10 cm × 10 cm (Merck, Darmstadt, Germany) were used in the experiments.

    Techniques: Migration, High Performance Thin Layer Chromatography, Thin Layer Chromatography

    Analysis of L-DOPA. (a) HPTLC. 1. Standard tyrosine. 2. Standard L-DOPA. 3. Biotransformation product. (b) Analysis of L-DOPA using HPLC. 1. Standard L-DOPA. 2. Biotransformation product.

    Journal: SpringerPlus

    Article Title: Statistically optimized biotransformation protocol for continuous production of L-DOPA using Mucuna monosperma callus culture

    doi: 10.1186/2193-1801-2-570

    Figure Lengend Snippet: Analysis of L-DOPA. (a) HPTLC. 1. Standard tyrosine. 2. Standard L-DOPA. 3. Biotransformation product. (b) Analysis of L-DOPA using HPLC. 1. Standard L-DOPA. 2. Biotransformation product.

    Article Snippet: The 10 μl of the standard L-DOPA and biotransformation product were loaded on pre-coated HPTLC plates (Silica gel 60 F 254, Merck, Germany), by using spray gas nitrogen and TLC sample loading instrument (CAMAG LINOMAT 5).

    Techniques: High Performance Thin Layer Chromatography, High Performance Liquid Chromatography