hpse proteinase k agarose beads  (Millipore)


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    Structured Review

    Millipore hpse proteinase k agarose beads
    <t>Proteinase-K</t> treated <t>HPSE</t> results in a reduced cytokine response in PBMCs. HPSE (1 µg) was treated with proteinase-K agarose beads (80 µg/ml) for 30 min at 37°C before removal of proteinase-K agarose beads and SDS-PAGE analysis. ( A ) Western blot analysis for HPSE confirms that proteinase-K treatment dramatically reduced intact HPSE levels in the sample. ( B ) HPSE activity assay on identically treated samples of either HPSE alone, proteinase K alone, or HPSE treated with proteinase K. Data represent the mean ±SEM (n = 3). ( C ) Expression of cytokines after stimulation with proteinase-K treated HPSE in IL-1β, IL-6, IL-10, IL-8 and TNF in PBMCs isolated from human whole blood. Data represent the mean ±SEM of triplicate samples, results are representative of three independent experiments. *p =
    Hpse Proteinase K Agarose Beads, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hpse proteinase k agarose beads/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hpse proteinase k agarose beads - by Bioz Stars, 2020-04
    85/100 stars

    Related Products / Commonly Used Together

    endotoxin free pbs
    proteinase-k treatment

    Images

    1) Product Images from "Soluble Heparan Sulfate Fragments Generated by Heparanase Trigger the Release of Pro-Inflammatory Cytokines through TLR-4"

    Article Title: Soluble Heparan Sulfate Fragments Generated by Heparanase Trigger the Release of Pro-Inflammatory Cytokines through TLR-4

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0109596

    Proteinase-K treated HPSE results in a reduced cytokine response in PBMCs. HPSE (1 µg) was treated with proteinase-K agarose beads (80 µg/ml) for 30 min at 37°C before removal of proteinase-K agarose beads and SDS-PAGE analysis. ( A ) Western blot analysis for HPSE confirms that proteinase-K treatment dramatically reduced intact HPSE levels in the sample. ( B ) HPSE activity assay on identically treated samples of either HPSE alone, proteinase K alone, or HPSE treated with proteinase K. Data represent the mean ±SEM (n = 3). ( C ) Expression of cytokines after stimulation with proteinase-K treated HPSE in IL-1β, IL-6, IL-10, IL-8 and TNF in PBMCs isolated from human whole blood. Data represent the mean ±SEM of triplicate samples, results are representative of three independent experiments. *p =
    Figure Legend Snippet: Proteinase-K treated HPSE results in a reduced cytokine response in PBMCs. HPSE (1 µg) was treated with proteinase-K agarose beads (80 µg/ml) for 30 min at 37°C before removal of proteinase-K agarose beads and SDS-PAGE analysis. ( A ) Western blot analysis for HPSE confirms that proteinase-K treatment dramatically reduced intact HPSE levels in the sample. ( B ) HPSE activity assay on identically treated samples of either HPSE alone, proteinase K alone, or HPSE treated with proteinase K. Data represent the mean ±SEM (n = 3). ( C ) Expression of cytokines after stimulation with proteinase-K treated HPSE in IL-1β, IL-6, IL-10, IL-8 and TNF in PBMCs isolated from human whole blood. Data represent the mean ±SEM of triplicate samples, results are representative of three independent experiments. *p =

    Techniques Used: SDS Page, Western Blot, Activity Assay, Expressing, Isolation

    Related Articles

    Concentration Assay:

    Article Title: Soluble Heparan Sulfate Fragments Generated by Heparanase Trigger the Release of Pro-Inflammatory Cytokines through TLR-4
    Article Snippet: .. Proteinase-K treatment of HPSE Proteinase-K-agarose beads (Sigma Aldrich) were suspended in endotoxin free PBS (Ca2+ - and Mg2+ -free, Life Technologies, CA) to a concentration of 80 mg/ml. ..

    Centrifugation:

    Article Title: Soluble Heparan Sulfate Fragments Generated by Heparanase Trigger the Release of Pro-Inflammatory Cytokines through TLR-4
    Article Snippet: Proteinase-K treatment of HPSE Proteinase-K-agarose beads (Sigma Aldrich) were suspended in endotoxin free PBS (Ca2+ - and Mg2+ -free, Life Technologies, CA) to a concentration of 80 mg/ml. .. The samples were transferred immediately to ice, before the proteinase-K beads were removed via centrifugation at 200 g for 3 min at 4°C.

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    Millipore heparanase
    Enzymatically active <t>heparanase</t> is expressed by DI MNCs in NOD mice. ( A ) H E and Alcian blue staining of NOD/Lt islets demonstrated that intact islets without insulitis (I) or islets with NDI contained extensive HS. In contrast, DI (arrow) was associated with local disruption of islet HS (asterisks). Scale bars: 100 μm. ( B ) Quantification of islet area stained by Alcian blue showed a significant reduction in islet-associated HS in prediabetic (PD) and onset-diabetic (OD) NOD/Lt mice compared with NOD/SCID (Sc) mice. Y, young 4- to 5-week-old NOD/Lt donors. n = 60–70 islets/group, 6–7 pancreases/group. Data are mean ± SEM. * P
    Heparanase, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/heparanase/product/Millipore
    Average 92 stars, based on 5 article reviews
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    heparanase - by Bioz Stars, 2020-04
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    84
    Millipore hpse high cells
    Enzymatically active <t>heparanase</t> is expressed by DI MNCs in NOD mice. ( A ) H E and Alcian blue staining of NOD/Lt islets demonstrated that intact islets without insulitis (I) or islets with NDI contained extensive HS. In contrast, DI (arrow) was associated with local disruption of islet HS (asterisks). Scale bars: 100 μm. ( B ) Quantification of islet area stained by Alcian blue showed a significant reduction in islet-associated HS in prediabetic (PD) and onset-diabetic (OD) NOD/Lt mice compared with NOD/SCID (Sc) mice. Y, young 4- to 5-week-old NOD/Lt donors. n = 60–70 islets/group, 6–7 pancreases/group. Data are mean ± SEM. * P
    Hpse High Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hpse high cells/product/Millipore
    Average 84 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    hpse high cells - by Bioz Stars, 2020-04
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    Image Search Results


    Enzymatically active heparanase is expressed by DI MNCs in NOD mice. ( A ) H E and Alcian blue staining of NOD/Lt islets demonstrated that intact islets without insulitis (I) or islets with NDI contained extensive HS. In contrast, DI (arrow) was associated with local disruption of islet HS (asterisks). Scale bars: 100 μm. ( B ) Quantification of islet area stained by Alcian blue showed a significant reduction in islet-associated HS in prediabetic (PD) and onset-diabetic (OD) NOD/Lt mice compared with NOD/SCID (Sc) mice. Y, young 4- to 5-week-old NOD/Lt donors. n = 60–70 islets/group, 6–7 pancreases/group. Data are mean ± SEM. * P

    Journal: The Journal of Clinical Investigation

    Article Title: Heparan sulfate and heparanase play key roles in mouse ? cell survival and autoimmune diabetes

    doi: 10.1172/JCI46177

    Figure Lengend Snippet: Enzymatically active heparanase is expressed by DI MNCs in NOD mice. ( A ) H E and Alcian blue staining of NOD/Lt islets demonstrated that intact islets without insulitis (I) or islets with NDI contained extensive HS. In contrast, DI (arrow) was associated with local disruption of islet HS (asterisks). Scale bars: 100 μm. ( B ) Quantification of islet area stained by Alcian blue showed a significant reduction in islet-associated HS in prediabetic (PD) and onset-diabetic (OD) NOD/Lt mice compared with NOD/SCID (Sc) mice. Y, young 4- to 5-week-old NOD/Lt donors. n = 60–70 islets/group, 6–7 pancreases/group. Data are mean ± SEM. * P

    Article Snippet: We localized HS (10E4 or HepSS-1 mAbs) and heparanase (HP-130 mAb) by immunohistochemistry after antigen retrieval using 0.05% pronase protease (Calbiochem) for HS ( ) or heat-induced standard antigen retrieval for heparanase , using the M.O.M. immunodetection kit (Vector Laboratories).

    Techniques: Mouse Assay, Staining

    Inhibition of heparanase activity in vivo protects NOD mice from T1D and destructive immunity. ( A ) NOD/Lt female mice (10.5–11 weeks) were injected daily i.p. with 10 mg/kg PI-88 (black; n = 23 per group) or saline (white; n = 25 per group). PI-88 treatment delayed the onset of diabetes by 70 days and reduced the incidence of diabetes to 30% compared with 62% in control mice. # P = 0.0039, * P = 0.0415 vs. saline. ( B ) Pancreases from nondiabetic PI-88– (black; n = 4 per group) or saline-treated (white; n = 3 per group) NOD/Lt mice at 253 days of age (from A ) were analyzed histologically to assess the level of insulitis. PI-88 treatment significantly increased the percentage of islets lacking insulitis (normal) and significantly decreased the percentage of islets with DI, with a noticeable reduction also observed in completely destroyed (CD) islets. Results are mean ± SEM. * P = 0.0026; ** P = 0.0244. ( C ) Additional serial pancreas sections from B were alternately stained with H E and Alcian blue. PI-88 treatment preserved islet-associated HS in the presence of DI, compared with control islets from saline-treated mice, in which intraislet HS was severely disrupted. Scale bars: 100 μm. ( D ) Alcian blue staining intensity was significantly stronger in islets with DI from PI-88–treated mice than from control saline-treated mice from B ( P = 0.0009), as quantified by Image J with Color Deconvolution plugin. Results are mean ± SD.

    Journal: The Journal of Clinical Investigation

    Article Title: Heparan sulfate and heparanase play key roles in mouse ? cell survival and autoimmune diabetes

    doi: 10.1172/JCI46177

    Figure Lengend Snippet: Inhibition of heparanase activity in vivo protects NOD mice from T1D and destructive immunity. ( A ) NOD/Lt female mice (10.5–11 weeks) were injected daily i.p. with 10 mg/kg PI-88 (black; n = 23 per group) or saline (white; n = 25 per group). PI-88 treatment delayed the onset of diabetes by 70 days and reduced the incidence of diabetes to 30% compared with 62% in control mice. # P = 0.0039, * P = 0.0415 vs. saline. ( B ) Pancreases from nondiabetic PI-88– (black; n = 4 per group) or saline-treated (white; n = 3 per group) NOD/Lt mice at 253 days of age (from A ) were analyzed histologically to assess the level of insulitis. PI-88 treatment significantly increased the percentage of islets lacking insulitis (normal) and significantly decreased the percentage of islets with DI, with a noticeable reduction also observed in completely destroyed (CD) islets. Results are mean ± SEM. * P = 0.0026; ** P = 0.0244. ( C ) Additional serial pancreas sections from B were alternately stained with H E and Alcian blue. PI-88 treatment preserved islet-associated HS in the presence of DI, compared with control islets from saline-treated mice, in which intraislet HS was severely disrupted. Scale bars: 100 μm. ( D ) Alcian blue staining intensity was significantly stronger in islets with DI from PI-88–treated mice than from control saline-treated mice from B ( P = 0.0009), as quantified by Image J with Color Deconvolution plugin. Results are mean ± SD.

    Article Snippet: We localized HS (10E4 or HepSS-1 mAbs) and heparanase (HP-130 mAb) by immunohistochemistry after antigen retrieval using 0.05% pronase protease (Calbiochem) for HS ( ) or heat-induced standard antigen retrieval for heparanase , using the M.O.M. immunodetection kit (Vector Laboratories).

    Techniques: Inhibition, Activity Assay, In Vivo, Mouse Assay, Injection, Staining