hplc  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    High Performance Liquid Chromatography
    Description:

    Catalog Number:
    h8147
    Price:
    None
    Buy from Supplier


    Structured Review

    Millipore hplc

    https://www.bioz.com/result/hplc/product/Millipore
    Average 99 stars, based on 895 article reviews
    Price from $9.99 to $1999.99
    hplc - by Bioz Stars, 2020-09
    99/100 stars

    Images

    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Dual positional substrate specificity of rice allene oxide synthase-1: insight into mechanism of inhibition by type II ligand imidazole
    Article Snippet: .. Products (ketols and cyclopentanone derivatives) of 13-Soybean LOX/OsAOS1 reaction collected from normal phase HPLC were derivatized with Sigma-Sil-A (Sigma, USA) at 80℃ for 5 min. Products from CaLOX1/OsAOS1 reaction were initially methylated with (trimethylsilyl)diazomethane, and/or then derivatized with TMSiCl. .. Methylated and/or trimethylsilylated products were then analyzed by GC-MS (Elite-5MS, 0.25 mm × 30 m, 0.25 μm film thickness, Perkin Elmer, USA) with an injector temperature of 260℃.

    Article Title: A Novel Metagenomic Short-Chain Dehydrogenase/Reductase Attenuates Pseudomonas aeruginosa Biofilm Formation and Virulence on Caenorhabditis elegans
    Article Snippet: .. HPLC-MS analysis For chemical analysis 2 µmol 3-oxo-C12 -HSL (Sigma-Aldrich, Heidelberg, Germany) (4 mM final concentration in 0.5 ml total volume) was mixed with 1 mM NADPH and 100 µg purified BpiB09 protein in 100 mM potassium phosphate buffer pH 7.0 with 20% DMSO as cosolvent and incubated for 16 h at 30°C. .. After incubation, the resulting mixtures were extracted twice with ethyl acetate (total of 2 volumes) and the combined organic layers were concentrated in vacuo.

    Article Title: Highly modified and immunoactive N-glycans of the canine heartworm
    Article Snippet: .. HPLC fractionation For 1D-HPLC, complete pyridylaminated N-glycomes were fractionated by reversed-phase HPLC (Ascentis Express RP-amide from Sigma-Aldrich; 150 × 4.6 mm, 2.7 µm) and a gradient of 30% (v/v) methanol (buffer B) in 100 mM ammonium acetate, pH 4 (buffer A) was applied at a flow rate of 0.8 ml/min (Shimadzu LC-30 AD pumps) as follows: 0–4 min, 0% B; 4–14 min, 0–5% B; 14–24 min, 5–15% B; 24–34 min, 15–35% B; 34–35 min, return to starting conditions . .. The RP-amide HPLC column was calibrated daily in terms of glucose units using a pyridylaminated dextran hydrolysate and the degree of polymerisation of single standards was verified by MALDI-TOF–MS .

    Article Title: N-Acetyl Cysteine (NAC)-Directed Detoxification of Methacryloxylethyl Cetyl Ammonium Chloride (DMAE-CB)
    Article Snippet: .. Analysis of DMAE-CB-NAC adduct formation with high performance liquid chromatography (HPLC) Pure DMAE-CB was added into 10 mM NAC (Sigma; St Louis, MO, USA) solution (in water) to a final concentration of 8 mM. ..

    Article Title: Molecular breeding of a novel orange-brown tomato fruit with enhanced beta-carotene and chlorophyll accumulation
    Article Snippet: .. HPLC analysis Standards for β-carotene, lycopene, phytoene, and chlorophyll were purchased from Sigma- Aldrich (Sigma Co., USA). ..

    Article Title: The function of two P450s, CYP9M10 and CYP6AA7, in the permethrin resistance of Culex quinquefasciatus
    Article Snippet: .. HPLC analysis and permethrin, PBOH, and PBCHO metabolism study Permethrin, PBOH and PBCHO (HPLC grade, Sigma Aldrich) were initially dissolved in acetonitrile and PBCOOH (HPLC grade, Sigma Aldrich) in methanol to create 1 mM standard solutions. ..

    Article Title: Effects of Mlx-8, a phospholipase A2 from Brazilian coralsnake Micrurus lemniscatus venom, on muscarinic acetylcholine receptors in rat hippocampus
    Article Snippet: .. Drugs and Radiochemicals Carbachol (carbamylcholine chloride), lithium chloride, myo-inositol, pirenzepine (pirenzepine hydrochloride), HPLC grade acetonitrile, and trifluoroacetic acid were obtained from Sigma Chemical Co. (USA). .. The 7,7-dimethyl-5,8-eicosadienoic acid (DEDA) was obtained from Abcam Laboratories (USA).

    Hydrophilic Interaction Liquid Chromatography:

    Article Title: N-glycosylation of Colorectal Cancer Tissues
    Article Snippet: .. Five μl of dried and reconstituted HILIC-HPLC fractions were desalted using a C18 ZipTipTM (Millipore, Billerica, MA) following the manufacturer's instructions. .. AA-labeled glycans were eluted with 1.5 μl of 2,5-dihydroxybenzoic acid (10 mg/ml in 50/50, ACN/water containing 0.1% TFA) directly onto a stainless steel MALDI target plate and allowed to dry.

    Flow Cytometry:

    Article Title: Highly modified and immunoactive N-glycans of the canine heartworm
    Article Snippet: .. HPLC fractionation For 1D-HPLC, complete pyridylaminated N-glycomes were fractionated by reversed-phase HPLC (Ascentis Express RP-amide from Sigma-Aldrich; 150 × 4.6 mm, 2.7 µm) and a gradient of 30% (v/v) methanol (buffer B) in 100 mM ammonium acetate, pH 4 (buffer A) was applied at a flow rate of 0.8 ml/min (Shimadzu LC-30 AD pumps) as follows: 0–4 min, 0% B; 4–14 min, 0–5% B; 14–24 min, 5–15% B; 24–34 min, 15–35% B; 34–35 min, return to starting conditions . .. The RP-amide HPLC column was calibrated daily in terms of glucose units using a pyridylaminated dextran hydrolysate and the degree of polymerisation of single standards was verified by MALDI-TOF–MS .

    Methylation:

    Article Title: Dual positional substrate specificity of rice allene oxide synthase-1: insight into mechanism of inhibition by type II ligand imidazole
    Article Snippet: .. Products (ketols and cyclopentanone derivatives) of 13-Soybean LOX/OsAOS1 reaction collected from normal phase HPLC were derivatized with Sigma-Sil-A (Sigma, USA) at 80℃ for 5 min. Products from CaLOX1/OsAOS1 reaction were initially methylated with (trimethylsilyl)diazomethane, and/or then derivatized with TMSiCl. .. Methylated and/or trimethylsilylated products were then analyzed by GC-MS (Elite-5MS, 0.25 mm × 30 m, 0.25 μm film thickness, Perkin Elmer, USA) with an injector temperature of 260℃.

    Purification:

    Article Title: A Novel Metagenomic Short-Chain Dehydrogenase/Reductase Attenuates Pseudomonas aeruginosa Biofilm Formation and Virulence on Caenorhabditis elegans
    Article Snippet: .. HPLC-MS analysis For chemical analysis 2 µmol 3-oxo-C12 -HSL (Sigma-Aldrich, Heidelberg, Germany) (4 mM final concentration in 0.5 ml total volume) was mixed with 1 mM NADPH and 100 µg purified BpiB09 protein in 100 mM potassium phosphate buffer pH 7.0 with 20% DMSO as cosolvent and incubated for 16 h at 30°C. .. After incubation, the resulting mixtures were extracted twice with ethyl acetate (total of 2 volumes) and the combined organic layers were concentrated in vacuo.

    Concentration Assay:

    Article Title: A Novel Metagenomic Short-Chain Dehydrogenase/Reductase Attenuates Pseudomonas aeruginosa Biofilm Formation and Virulence on Caenorhabditis elegans
    Article Snippet: .. HPLC-MS analysis For chemical analysis 2 µmol 3-oxo-C12 -HSL (Sigma-Aldrich, Heidelberg, Germany) (4 mM final concentration in 0.5 ml total volume) was mixed with 1 mM NADPH and 100 µg purified BpiB09 protein in 100 mM potassium phosphate buffer pH 7.0 with 20% DMSO as cosolvent and incubated for 16 h at 30°C. .. After incubation, the resulting mixtures were extracted twice with ethyl acetate (total of 2 volumes) and the combined organic layers were concentrated in vacuo.

    Article Title: N-Acetyl Cysteine (NAC)-Directed Detoxification of Methacryloxylethyl Cetyl Ammonium Chloride (DMAE-CB)
    Article Snippet: .. Analysis of DMAE-CB-NAC adduct formation with high performance liquid chromatography (HPLC) Pure DMAE-CB was added into 10 mM NAC (Sigma; St Louis, MO, USA) solution (in water) to a final concentration of 8 mM. ..

    Incubation:

    Article Title: A Novel Metagenomic Short-Chain Dehydrogenase/Reductase Attenuates Pseudomonas aeruginosa Biofilm Formation and Virulence on Caenorhabditis elegans
    Article Snippet: .. HPLC-MS analysis For chemical analysis 2 µmol 3-oxo-C12 -HSL (Sigma-Aldrich, Heidelberg, Germany) (4 mM final concentration in 0.5 ml total volume) was mixed with 1 mM NADPH and 100 µg purified BpiB09 protein in 100 mM potassium phosphate buffer pH 7.0 with 20% DMSO as cosolvent and incubated for 16 h at 30°C. .. After incubation, the resulting mixtures were extracted twice with ethyl acetate (total of 2 volumes) and the combined organic layers were concentrated in vacuo.

    Fractionation:

    Article Title: Highly modified and immunoactive N-glycans of the canine heartworm
    Article Snippet: .. HPLC fractionation For 1D-HPLC, complete pyridylaminated N-glycomes were fractionated by reversed-phase HPLC (Ascentis Express RP-amide from Sigma-Aldrich; 150 × 4.6 mm, 2.7 µm) and a gradient of 30% (v/v) methanol (buffer B) in 100 mM ammonium acetate, pH 4 (buffer A) was applied at a flow rate of 0.8 ml/min (Shimadzu LC-30 AD pumps) as follows: 0–4 min, 0% B; 4–14 min, 0–5% B; 14–24 min, 5–15% B; 24–34 min, 15–35% B; 34–35 min, return to starting conditions . .. The RP-amide HPLC column was calibrated daily in terms of glucose units using a pyridylaminated dextran hydrolysate and the degree of polymerisation of single standards was verified by MALDI-TOF–MS .

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Millipore lcs 1
    SOD1 is necessary for oncogene-driven proliferation of primary mouse epithelial cells. (a) Immunoblotting of MCL1-s, p53, and p16 of SOD1 +/+, SOD1 +/−, and SOD1 −/− iErBb2 mammary gland. (b) Quantification of MCL1-s, p53, and p16 expression from (a) relative to loading. (c) Representative images of immunohistochemistry of p16 in SOD +/+, SOD +/−, and SOD −/− iErbB2 mammary glands. (d) Quantification of percentage of positive p16 stain from (c). (e) Brightfield images of 3D primary mammary epithelial organoids from SOD +/+ and SOD +/− iErbB2 mice in the presence doxycycline at Day 1 (n=5), 4 (n=5), and 7 (n=5). Scale bar represents 100 uM. (f) Measurements of diameter of 3D organoids from (e). (g) Percentage of organoids from (e) with ErbB2 driven morphogenesis (n=3, 10 observations per time point). (h) Immunofluorescence of MCL-1 (green) in SOD +/+ and SOD +/− erbB2 organoids. Nuclei were stained with DAPI. (i) Representative images of Senescence-Associated-β-galactosidase (SA-β-gal) staining of SOD +/+ and SOD +/− iErbB2 mammary epithelial cells. (j) Percentage of SA-β-gal positive cells. (k) Representative images of colony formation assay or iErb2 tumor cells treated with DMSO (Control) or SOD1 inhibitor <t>LCS-1</t> (n = 5). (l) Quantification of colony formation assay in k. (m) MTT Proliferation assay of iErb2 tumor cells treated with DMSO (Control) or LCS-1 (n = 3).
    Lcs 1, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lcs 1/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lcs 1 - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    84
    Millipore hamilton hplc anion exchange column
    scsHi-C methodology based on nascent <t>DNA</t> labeling in live cells. (a) Sister chromatid-specific labeling using synthetic nucleotides. During DNA replication, a synthetic nucleotide analogue incorporates into different strands (Watson or Crick) within each sister chromatid. Labelled DNA, dashed line; unlabelled DNA, solid line. (b) Strategy to distinguish cis from trans sister contacts in a Hi-C experiment based on 4sT-mediated DNA labeling. After progression through S-Phase in the presence of 4sT, each sister chromatid contains one labelled DNA strand of opposing strandedness (see panel a). Chromatin is crosslinked in cells and Hi-C samples are prepared using standard procedures, followed by chemical conversion to induce 4sT signature mutations and Illumina-based sequencing. Half-reads are classified as labelled if at least two signature mutations are present. If a ligation junction contains two labelled half-reads that map to the same strand, it is classified as cis sister contact; if it contains two labelled halves that map to opposing strands, it is classified as a trans sister contact (see Extended Data Fig. 3c for details). (c) Conversion of 4-thio-thymidine (4sT) to the point-mutation inducing 5-methyl-Cytosine (5mC) by treating DNA with OsO 4 and NH 4 Cl at elevated temperatures. Functional groups that are changed in the course of the reaction are highlighted in red. (d) Synthetic hairpin-oligonucleotide used to probe 4sT conversion by OsO 4 . The theorized reaction educts and products are highlighted in red. (e) High performance liquid chromatography <t>(HPLC)</t> trace at 260 nm of the oligos depicted in (d) before and after the conversion by OsO 4 /NH 4 Cl. The peak position of the oligo before conversion is indicated by a dashed line. (f) Point-mutation rates of genomic DNA from HeLa cells grown in medium containing 4sT relative to control DNA from cells grown in the absence of 4sT, before and after OsO 4 /NH 4 Cl-mediated conversion. Bar graphs indicate the mean and standard error of three independent experiments. (g) Experimental procedure for differential labeling of sister chromatids using 4sT. See Extended Data Fig. 2c for more details. (h) Quantification Hi-C reads that are labelled on both sides for contact sister-specificity classification, as a percentage of all reads. Cells were synchronized to the G1/S boundary and released into S-Phase in the presence of 4sT for the indicated times. The G2 sample was arrested using RO3306; the control sample refers to unlabelled DNA. Bars show the mean of two biological replicates. (i) Percentage of trans sister contacts based on all double-labelled reads that exhibit a genomic separation larger than 10kb. Cells were released from G1/S block into medium containing 4sT and then arrested in G2 using RO3306, in mitosis using nocodazole, or the following G1 using thymidine. Bars show mean of two biological replicates.
    Hamilton Hplc Anion Exchange Column, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hamilton hplc anion exchange column/product/Millipore
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hamilton hplc anion exchange column - by Bioz Stars, 2020-09
    84/100 stars
      Buy from Supplier

    84
    Millipore hplc methods for pharmaceutical analysis
    <t>HPLC</t> chromatograms showing the same retention time (5.85 min, shown with arrow) for the ST-S and the MUS1 spots obtained in the TLC assay. Both spots were scrapped from the silica, purified and analyzed using reverse-phase HPLC. ST-S is the authentic <t>Taxol</t> standard while MUS1 corresponds to the putative Taxol compound obtained from the EA fraction of isolate MUS1 .
    Hplc Methods For Pharmaceutical Analysis, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc methods for pharmaceutical analysis/product/Millipore
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hplc methods for pharmaceutical analysis - by Bioz Stars, 2020-09
    84/100 stars
      Buy from Supplier

    Image Search Results


    SOD1 is necessary for oncogene-driven proliferation of primary mouse epithelial cells. (a) Immunoblotting of MCL1-s, p53, and p16 of SOD1 +/+, SOD1 +/−, and SOD1 −/− iErBb2 mammary gland. (b) Quantification of MCL1-s, p53, and p16 expression from (a) relative to loading. (c) Representative images of immunohistochemistry of p16 in SOD +/+, SOD +/−, and SOD −/− iErbB2 mammary glands. (d) Quantification of percentage of positive p16 stain from (c). (e) Brightfield images of 3D primary mammary epithelial organoids from SOD +/+ and SOD +/− iErbB2 mice in the presence doxycycline at Day 1 (n=5), 4 (n=5), and 7 (n=5). Scale bar represents 100 uM. (f) Measurements of diameter of 3D organoids from (e). (g) Percentage of organoids from (e) with ErbB2 driven morphogenesis (n=3, 10 observations per time point). (h) Immunofluorescence of MCL-1 (green) in SOD +/+ and SOD +/− erbB2 organoids. Nuclei were stained with DAPI. (i) Representative images of Senescence-Associated-β-galactosidase (SA-β-gal) staining of SOD +/+ and SOD +/− iErbB2 mammary epithelial cells. (j) Percentage of SA-β-gal positive cells. (k) Representative images of colony formation assay or iErb2 tumor cells treated with DMSO (Control) or SOD1 inhibitor LCS-1 (n = 5). (l) Quantification of colony formation assay in k. (m) MTT Proliferation assay of iErb2 tumor cells treated with DMSO (Control) or LCS-1 (n = 3).

    Journal: Oncogene

    Article Title: SOD1 is essential for oncogene-driven mammary tumor formation but dispensable for normal development and proliferation.

    doi: 10.1038/s41388-019-0839-x

    Figure Lengend Snippet: SOD1 is necessary for oncogene-driven proliferation of primary mouse epithelial cells. (a) Immunoblotting of MCL1-s, p53, and p16 of SOD1 +/+, SOD1 +/−, and SOD1 −/− iErBb2 mammary gland. (b) Quantification of MCL1-s, p53, and p16 expression from (a) relative to loading. (c) Representative images of immunohistochemistry of p16 in SOD +/+, SOD +/−, and SOD −/− iErbB2 mammary glands. (d) Quantification of percentage of positive p16 stain from (c). (e) Brightfield images of 3D primary mammary epithelial organoids from SOD +/+ and SOD +/− iErbB2 mice in the presence doxycycline at Day 1 (n=5), 4 (n=5), and 7 (n=5). Scale bar represents 100 uM. (f) Measurements of diameter of 3D organoids from (e). (g) Percentage of organoids from (e) with ErbB2 driven morphogenesis (n=3, 10 observations per time point). (h) Immunofluorescence of MCL-1 (green) in SOD +/+ and SOD +/− erbB2 organoids. Nuclei were stained with DAPI. (i) Representative images of Senescence-Associated-β-galactosidase (SA-β-gal) staining of SOD +/+ and SOD +/− iErbB2 mammary epithelial cells. (j) Percentage of SA-β-gal positive cells. (k) Representative images of colony formation assay or iErb2 tumor cells treated with DMSO (Control) or SOD1 inhibitor LCS-1 (n = 5). (l) Quantification of colony formation assay in k. (m) MTT Proliferation assay of iErb2 tumor cells treated with DMSO (Control) or LCS-1 (n = 3).

    Article Snippet: Cells were treated with either dimethyl sufoxide (Sigma Aldrich) or 3 μM LCS-1 (EMB Millipore, San Diego, CA, USA, 567417).

    Techniques: Expressing, Immunohistochemistry, Staining, Mouse Assay, Immunofluorescence, Colony Assay, MTT Assay, Proliferation Assay

    scsHi-C methodology based on nascent DNA labeling in live cells. (a) Sister chromatid-specific labeling using synthetic nucleotides. During DNA replication, a synthetic nucleotide analogue incorporates into different strands (Watson or Crick) within each sister chromatid. Labelled DNA, dashed line; unlabelled DNA, solid line. (b) Strategy to distinguish cis from trans sister contacts in a Hi-C experiment based on 4sT-mediated DNA labeling. After progression through S-Phase in the presence of 4sT, each sister chromatid contains one labelled DNA strand of opposing strandedness (see panel a). Chromatin is crosslinked in cells and Hi-C samples are prepared using standard procedures, followed by chemical conversion to induce 4sT signature mutations and Illumina-based sequencing. Half-reads are classified as labelled if at least two signature mutations are present. If a ligation junction contains two labelled half-reads that map to the same strand, it is classified as cis sister contact; if it contains two labelled halves that map to opposing strands, it is classified as a trans sister contact (see Extended Data Fig. 3c for details). (c) Conversion of 4-thio-thymidine (4sT) to the point-mutation inducing 5-methyl-Cytosine (5mC) by treating DNA with OsO 4 and NH 4 Cl at elevated temperatures. Functional groups that are changed in the course of the reaction are highlighted in red. (d) Synthetic hairpin-oligonucleotide used to probe 4sT conversion by OsO 4 . The theorized reaction educts and products are highlighted in red. (e) High performance liquid chromatography (HPLC) trace at 260 nm of the oligos depicted in (d) before and after the conversion by OsO 4 /NH 4 Cl. The peak position of the oligo before conversion is indicated by a dashed line. (f) Point-mutation rates of genomic DNA from HeLa cells grown in medium containing 4sT relative to control DNA from cells grown in the absence of 4sT, before and after OsO 4 /NH 4 Cl-mediated conversion. Bar graphs indicate the mean and standard error of three independent experiments. (g) Experimental procedure for differential labeling of sister chromatids using 4sT. See Extended Data Fig. 2c for more details. (h) Quantification Hi-C reads that are labelled on both sides for contact sister-specificity classification, as a percentage of all reads. Cells were synchronized to the G1/S boundary and released into S-Phase in the presence of 4sT for the indicated times. The G2 sample was arrested using RO3306; the control sample refers to unlabelled DNA. Bars show the mean of two biological replicates. (i) Percentage of trans sister contacts based on all double-labelled reads that exhibit a genomic separation larger than 10kb. Cells were released from G1/S block into medium containing 4sT and then arrested in G2 using RO3306, in mitosis using nocodazole, or the following G1 using thymidine. Bars show mean of two biological replicates.

    Journal: bioRxiv

    Article Title: Sister-chromatid-sensitive Hi-C reveals the conformation of replicated human chromosomes

    doi: 10.1101/2020.03.10.978148

    Figure Lengend Snippet: scsHi-C methodology based on nascent DNA labeling in live cells. (a) Sister chromatid-specific labeling using synthetic nucleotides. During DNA replication, a synthetic nucleotide analogue incorporates into different strands (Watson or Crick) within each sister chromatid. Labelled DNA, dashed line; unlabelled DNA, solid line. (b) Strategy to distinguish cis from trans sister contacts in a Hi-C experiment based on 4sT-mediated DNA labeling. After progression through S-Phase in the presence of 4sT, each sister chromatid contains one labelled DNA strand of opposing strandedness (see panel a). Chromatin is crosslinked in cells and Hi-C samples are prepared using standard procedures, followed by chemical conversion to induce 4sT signature mutations and Illumina-based sequencing. Half-reads are classified as labelled if at least two signature mutations are present. If a ligation junction contains two labelled half-reads that map to the same strand, it is classified as cis sister contact; if it contains two labelled halves that map to opposing strands, it is classified as a trans sister contact (see Extended Data Fig. 3c for details). (c) Conversion of 4-thio-thymidine (4sT) to the point-mutation inducing 5-methyl-Cytosine (5mC) by treating DNA with OsO 4 and NH 4 Cl at elevated temperatures. Functional groups that are changed in the course of the reaction are highlighted in red. (d) Synthetic hairpin-oligonucleotide used to probe 4sT conversion by OsO 4 . The theorized reaction educts and products are highlighted in red. (e) High performance liquid chromatography (HPLC) trace at 260 nm of the oligos depicted in (d) before and after the conversion by OsO 4 /NH 4 Cl. The peak position of the oligo before conversion is indicated by a dashed line. (f) Point-mutation rates of genomic DNA from HeLa cells grown in medium containing 4sT relative to control DNA from cells grown in the absence of 4sT, before and after OsO 4 /NH 4 Cl-mediated conversion. Bar graphs indicate the mean and standard error of three independent experiments. (g) Experimental procedure for differential labeling of sister chromatids using 4sT. See Extended Data Fig. 2c for more details. (h) Quantification Hi-C reads that are labelled on both sides for contact sister-specificity classification, as a percentage of all reads. Cells were synchronized to the G1/S boundary and released into S-Phase in the presence of 4sT for the indicated times. The G2 sample was arrested using RO3306; the control sample refers to unlabelled DNA. Bars show the mean of two biological replicates. (i) Percentage of trans sister contacts based on all double-labelled reads that exhibit a genomic separation larger than 10kb. Cells were released from G1/S block into medium containing 4sT and then arrested in G2 using RO3306, in mitosis using nocodazole, or the following G1 using thymidine. Bars show mean of two biological replicates.

    Article Snippet: The supernatant was discarded, and the precipitated DNA analyzed by anion exchange HPLC and mass spectrometry (see above).

    Techniques: DNA Labeling, Labeling, Hi-C, Sequencing, Ligation, Mutagenesis, Functional Assay, High Performance Liquid Chromatography, Blocking Assay

    HPLC chromatograms showing the same retention time (5.85 min, shown with arrow) for the ST-S and the MUS1 spots obtained in the TLC assay. Both spots were scrapped from the silica, purified and analyzed using reverse-phase HPLC. ST-S is the authentic Taxol standard while MUS1 corresponds to the putative Taxol compound obtained from the EA fraction of isolate MUS1 .

    Journal: bioRxiv

    Article Title: Annulohypoxylon sp. strain MUS1, an Endophyte isolated from Taxus wallichiana Zucc. produces Taxol and Other Bioactive Metabolites

    doi: 10.1101/2020.04.05.025858

    Figure Lengend Snippet: HPLC chromatograms showing the same retention time (5.85 min, shown with arrow) for the ST-S and the MUS1 spots obtained in the TLC assay. Both spots were scrapped from the silica, purified and analyzed using reverse-phase HPLC. ST-S is the authentic Taxol standard while MUS1 corresponds to the putative Taxol compound obtained from the EA fraction of isolate MUS1 .

    Article Snippet: After centrifugation, the supernatant was taken and evaporated to dryness under reduced pressure and dissolved in methanol for the HPLC analysis of Taxol.

    Techniques: High Performance Liquid Chromatography, Thin Layer Chromatography, Purification