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Millipore hplc
Hplc, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 690 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hplc/product/Millipore
Average 99 stars, based on 690 article reviews
Price from $9.99 to $1999.99
hplc - by Bioz Stars, 2020-04
99/100 stars

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Diagnostic Assay:

Article Title: Producing aglycons of ginsenosides in bakers' yeast
Article Snippet: Compound separation was achieved with an injector temperature of 300°C and a 30 min temperature gradient program for GC-separation starting at 80°C for 1 min followed by heating the column to 300°C at 20°C min−1 and a final constant hold at 300°C for 15 min. Mass detection was achieved with electric ionisation using SIM-scan mode with diagnostic ions monitored as followed: m/z 69, m/z 218, m/z 363, m/z 411 and m/z 437. .. A crystallised β-amyrin sample was used as the standard for quantification (purchased from BioBioPha, China), and squalene, lanosterol and ergosterol standards (purchased from Sigma Aldrich) were also used for quantification.

Centrifugation:

Article Title: Producing aglycons of ginsenosides in bakers' yeast
Article Snippet: Chemical analysis Cells were collected from fermentation culture via centrifugation. .. A crystallised β-amyrin sample was used as the standard for quantification (purchased from BioBioPha, China), and squalene, lanosterol and ergosterol standards (purchased from Sigma Aldrich) were also used for quantification.

Proton NMR:

Article Title: Pneumocysterol [(24Z)-ethylidenelanost-8-en-3?-ol], a rare sterol detected in the opportunistic pathogen Pneumocystis carinii hominis: Structural identity and chemical synthesis
Article Snippet: Procedures and conditions for obtaining melting points, IR spectra, 1 H-NMR spectra, MS, TLC, GLC, and column chromatography were as described ( ). .. Commercial lanosterol (Sigma) was purified by using multiple ( ) recrystallization from acetone/water, and after recrystallization was found to be a mixture of lanosterol (Fig. , compound I ) and 24,25-dihydrolanosterol.

Incubation:

Article Title: Increased lanosterol turnover: a metabolic burden for daunorubicin-resistant leukemia cells
Article Snippet: Cells were incubated for 48 h with or without cholesterol biosynthesis inhibitors, namely, atorvastatin (100 µM), terbinafine (25 µM), ketoconazole (20 μM), triparanol (2 µM), CI976 (25 µM), all purchased from Sigma-Aldrich (MO, USA), and hymeglusin (10 µM), YM-53601 (10 µM) and BIBB-515 (25 µM) all ordered from Santa Cruz Biotechnology (TX, USA) in the absence (vehicle control DMSO or MeOH) or presence of DNR (CEM, 1 nM and CEM/R2, 0.5 μM) in RPMI 1640 (HyClone, Fisher scientific) supplemented with 10 % FBS. .. Lanosterol, cholesterol, and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (PC), all obtained from Sigma-Aldrich (MO, USA), were dissolved in chloroform/methanol (1:1).

Mass Spectrometry:

Article Title: Pneumocysterol [(24Z)-ethylidenelanost-8-en-3?-ol], a rare sterol detected in the opportunistic pathogen Pneumocystis carinii hominis: Structural identity and chemical synthesis
Article Snippet: Procedures and conditions for obtaining melting points, IR spectra, 1 H-NMR spectra, MS, TLC, GLC, and column chromatography were as described ( ). .. Commercial lanosterol (Sigma) was purified by using multiple ( ) recrystallization from acetone/water, and after recrystallization was found to be a mixture of lanosterol (Fig. , compound I ) and 24,25-dihydrolanosterol.

Western Blot:

Article Title: Progesterone Receptor Membrane Component 1 Inhibits the Activity of Drug-Metabolizing Cytochromes P450 and Binds to Cytochrome P450 Reductase
Article Snippet: The chemiluminescence Western blotting detection kit was obtained from Pierce Chemical Co. (Rockford, IL), and the P450-Glo kit for assaying P450 enzymatic activities was from Promega (Madison, WI). .. Sequabrene, pyridine, mevalonolactone, ergosterol, lanosterol, and anti-FLAG M2 affinity gel were from Sigma-Aldrich (St. Louis, MO).

Recrystallization:

Article Title: Pneumocysterol [(24Z)-ethylidenelanost-8-en-3?-ol], a rare sterol detected in the opportunistic pathogen Pneumocystis carinii hominis: Structural identity and chemical synthesis
Article Snippet: .. Commercial lanosterol (Sigma) was purified by using multiple ( ) recrystallization from acetone/water, and after recrystallization was found to be a mixture of lanosterol (Fig. , compound I ) and 24,25-dihydrolanosterol. ..

High Performance Liquid Chromatography:

Article Title: Producing aglycons of ginsenosides in bakers' yeast
Article Snippet: A crystallised β-amyrin sample was used as the standard for quantification (purchased from BioBioPha, China), and squalene, lanosterol and ergosterol standards (purchased from Sigma Aldrich) were also used for quantification. .. For the determination of oleanolic acid, acetone and methanol (1:1) extracts (20 μl) were analysed by LC/MS using an Agilent 1200 HPLC system coupled to a Bruker-micrOTOF-II with an electrospray ionisation (ESI) interface.

Gas Chromatography:

Article Title: Ergosterol Regulates Sterol Regulatory Element Binding Protein (SREBP) Cleavage in Fission Yeast *
Article Snippet: However, gas chromatography revealed that addition of this compound inhibited ergosterol synthesis ( B ). .. Consistent with this hypothesis, addition of pure lanosterol (Sigma L5768) did not induce Sre1 cleavage ( A , lanes 4–6 ) or inhibit ergosterol production ( B ), whereas addition of 24,25-dihydrolanosterol both blocked ergosterol synthesis ( B ) and induced Sre1 cleavage ( A , lanes 7–9 ).

Article Title: Pneumocysterol [(24Z)-ethylidenelanost-8-en-3?-ol], a rare sterol detected in the opportunistic pathogen Pneumocystis carinii hominis: Structural identity and chemical synthesis
Article Snippet: Procedures and conditions for obtaining melting points, IR spectra, 1 H-NMR spectra, MS, TLC, GLC, and column chromatography were as described ( ). .. Commercial lanosterol (Sigma) was purified by using multiple ( ) recrystallization from acetone/water, and after recrystallization was found to be a mixture of lanosterol (Fig. , compound I ) and 24,25-dihydrolanosterol.

Chromatography:

Article Title: Mesoionic compounds with antifungal activity against Fusarium verticillioides
Article Snippet: Chromatography was performed on silica gel 60 plates (Sigma Chemical Co., Missouri, USA), and the plates were developed with hexane:diethyl ether:acetic acid (60:30:1.5). .. Ergosterol (4 μg) and lanosterol (1 μg), which were purchased from Sigma Chemical Co. (Missouri, USA), were used as sterol standards.

Solubility:

Article Title: Universal Behavior of Membranes with Sterols
Article Snippet: POPC and cholesterol (98% pure) were obtained from Avanti Polar Lipids (Birmingham, AL), lanosterol (∼97% pure), and solvents from Sigma-Aldrich (Copenhagen, Denmark), and ergosterol (≥97% pure) from Fluka (Buchs, Switzerland). .. To achieve solubility, lanosterol and ergosterol were dissolved in 1:9 MeOH:CHCl3 and 1:3 MeOH:CHCl3 .

Inhibition:

Article Title: Ergosterol Regulates Sterol Regulatory Element Binding Protein (SREBP) Cleavage in Fission Yeast *
Article Snippet: This finding, together with recent data regarding this specific commercial preparation of lanosterol, suggested that this inhibition of sterol synthesis is not caused by lanosterol itself, but rather is caused by 24,25-dihydrolanosterol, a prominent contaminant of the commercial preparation ( ). .. Consistent with this hypothesis, addition of pure lanosterol (Sigma L5768) did not induce Sre1 cleavage ( A , lanes 4–6 ) or inhibit ergosterol production ( B ), whereas addition of 24,25-dihydrolanosterol both blocked ergosterol synthesis ( B ) and induced Sre1 cleavage ( A , lanes 7–9 ).

Purification:

Article Title: Differences in the Modulation of Collective Membrane Motions by Ergosterol, Lanosterol, and Cholesterol: A Dynamic Light Scattering Study
Article Snippet: The n -decane with 99+ % purity (Sigma-Aldrich, Steinheim, Germany) was additionally purified using an alumina column until it became completely colorless. .. Lanosterol (purity > 97%) and ergosterol (purity > 95%) were obtained from Sigma-Aldrich (Steinheim, Germany).

Article Title: Myosin 1g Contributes to CD44 Adhesion Protein and Lipid Rafts Recycling and Controls CD44 Capping and Cell Migration in B Lymphocytes
Article Snippet: .. Antibodies and reagents used in this study are as follows: purified rabbit polyclonal immunoglobulin (Ig)G α-Myo1g , rabbit α-Myo1g (Santa Cruz Biotechnology, Dallas, TX, USA), goat α-Myo1g (Santa Cruz Biotechnology), rat monoclonal antibodies (mAb) NIM-R1 (α-Thy-1) and NIM-R8 (α-CD44) , biotin-labeled mAb α-CD44 (clone IM7) (BD Pharmingen, San Diego, CA, USA), α-rat-PE (BD Pharmingen), purified α-goat-PE, α-rabbit IgG-Alexa488 (Molecular Probes, Eugene, OR, USA), α-rabbit IgG-FITC (Jackson ImmunoResearch, West Grove, PA, USA), α-rabbit IgG-Cy3 (Jackson ImmunoResearch), Streptavidin-Cy3 (Invitrogen, Frederick, MD, USA), TRITC-phalloidin (Molecular Probes), FITC-phalloidin (Sigma Chemical Co, St. Louis, MO, USA), Streptavidin-APC (BD), Pacific Blue α-B220 (BD Pharmingen) α-IgM (Zymed Laboratories, San Francisco, CA, USA), α-Rab5 (Santa Cruz Biotechnology), α-CDC42 (Santa Cruz Biotechnology), α-CDC42-GTP (New East Biosciences, Brownsville, TX, USA), α-RhoA (Santa Cruz Biotechnology), α-RhoA-GTP (New East Biosciences), α-Actin (Santa Cruz Biotechnology), mAb α-CD44 (clone KM201, Abcam Cambridge, UK), α-Ezrin (Santa Cruz Biotechnology), α-Caveolin (Santa Cruz Biotechnology), purified goat polyclonal IgG α-Rab11 , biotinylated cholera toxin subunit B (Life Technologies, Grand Island, NY), Streptavidin-FITC (Sigma), α-CD44-PE (BioLegend, San Diego, CA, USA), and α-B220-VB421 (BioLegend), Primaquine (Sigma), Lanosterol (Sigma), Cholesterol (Sigma), Golgi-stop (Becton Dickinson, East Rutherford, NJ, USA). .. B Lymphocyte Isolation, Activation, and Staining Mononuclear cells were isolated from the spleen by Ficoll-Hypaque density gradient separation.

Article Title: Sea cucumber genome provides insights into saponin biosynthesis and aestivation regulation
Article Snippet: .. Lanosterol (Cat# L5768) was purchased from Sigma-Aldrich, whereas parkeol and lanostadienol were purified and characterized according to published literatures , . .. The standards were dissolved and diluted to 0.5 mg/ml in hexane and used in GC-MS. Ketoconazole (Catlog # K1003) was purchased from Sigma dissolved in DMSO and used in yeast media.

Article Title: Pneumocysterol [(24Z)-ethylidenelanost-8-en-3?-ol], a rare sterol detected in the opportunistic pathogen Pneumocystis carinii hominis: Structural identity and chemical synthesis
Article Snippet: .. Commercial lanosterol (Sigma) was purified by using multiple ( ) recrystallization from acetone/water, and after recrystallization was found to be a mixture of lanosterol (Fig. , compound I ) and 24,25-dihydrolanosterol. ..

Mouse Assay:

Article Title: Myosin 1g Contributes to CD44 Adhesion Protein and Lipid Rafts Recycling and Controls CD44 Capping and Cell Migration in B Lymphocytes
Article Snippet: Paragraph title: Mice and Reagents ... Antibodies and reagents used in this study are as follows: purified rabbit polyclonal immunoglobulin (Ig)G α-Myo1g , rabbit α-Myo1g (Santa Cruz Biotechnology, Dallas, TX, USA), goat α-Myo1g (Santa Cruz Biotechnology), rat monoclonal antibodies (mAb) NIM-R1 (α-Thy-1) and NIM-R8 (α-CD44) , biotin-labeled mAb α-CD44 (clone IM7) (BD Pharmingen, San Diego, CA, USA), α-rat-PE (BD Pharmingen), purified α-goat-PE, α-rabbit IgG-Alexa488 (Molecular Probes, Eugene, OR, USA), α-rabbit IgG-FITC (Jackson ImmunoResearch, West Grove, PA, USA), α-rabbit IgG-Cy3 (Jackson ImmunoResearch), Streptavidin-Cy3 (Invitrogen, Frederick, MD, USA), TRITC-phalloidin (Molecular Probes), FITC-phalloidin (Sigma Chemical Co, St. Louis, MO, USA), Streptavidin-APC (BD), Pacific Blue α-B220 (BD Pharmingen) α-IgM (Zymed Laboratories, San Francisco, CA, USA), α-Rab5 (Santa Cruz Biotechnology), α-CDC42 (Santa Cruz Biotechnology), α-CDC42-GTP (New East Biosciences, Brownsville, TX, USA), α-RhoA (Santa Cruz Biotechnology), α-RhoA-GTP (New East Biosciences), α-Actin (Santa Cruz Biotechnology), mAb α-CD44 (clone KM201, Abcam Cambridge, UK), α-Ezrin (Santa Cruz Biotechnology), α-Caveolin (Santa Cruz Biotechnology), purified goat polyclonal IgG α-Rab11 , biotinylated cholera toxin subunit B (Life Technologies, Grand Island, NY), Streptavidin-FITC (Sigma), α-CD44-PE (BioLegend, San Diego, CA, USA), and α-B220-VB421 (BioLegend), Primaquine (Sigma), Lanosterol (Sigma), Cholesterol (Sigma), Golgi-stop (Becton Dickinson, East Rutherford, NJ, USA).

Software:

Article Title: Producing aglycons of ginsenosides in bakers' yeast
Article Snippet: A crystallised β-amyrin sample was used as the standard for quantification (purchased from BioBioPha, China), and squalene, lanosterol and ergosterol standards (purchased from Sigma Aldrich) were also used for quantification. .. Data acquisition and processing were performed with the MicrOTOF control version 3.0/Data Analysis Version 4.0 software.

Sample Prep:

Article Title: Universal Behavior of Membranes with Sterols
Article Snippet: Paragraph title: Sample preparation for micropipette aspiration ... POPC and cholesterol (98% pure) were obtained from Avanti Polar Lipids (Birmingham, AL), lanosterol (∼97% pure), and solvents from Sigma-Aldrich (Copenhagen, Denmark), and ergosterol (≥97% pure) from Fluka (Buchs, Switzerland).

Column Chromatography:

Article Title: Pneumocysterol [(24Z)-ethylidenelanost-8-en-3?-ol], a rare sterol detected in the opportunistic pathogen Pneumocystis carinii hominis: Structural identity and chemical synthesis
Article Snippet: Procedures and conditions for obtaining melting points, IR spectra, 1 H-NMR spectra, MS, TLC, GLC, and column chromatography were as described ( ). .. Commercial lanosterol (Sigma) was purified by using multiple ( ) recrystallization from acetone/water, and after recrystallization was found to be a mixture of lanosterol (Fig. , compound I ) and 24,25-dihydrolanosterol.

High Performance Thin Layer Chromatography:

Article Title: Mesoionic compounds with antifungal activity against Fusarium verticillioides
Article Snippet: The neutral lipids (lower phase) were dried under streaming N2 , redissolved in chloroform and spotted onto high performance thin-layer chromatography (HPTLC) plates. .. Ergosterol (4 μg) and lanosterol (1 μg), which were purchased from Sigma Chemical Co. (Missouri, USA), were used as sterol standards.

Produced:

Article Title: Myosin 1g Contributes to CD44 Adhesion Protein and Lipid Rafts Recycling and Controls CD44 Capping and Cell Migration in B Lymphocytes
Article Snippet: The mice were produced at the Centro de Investigación y de Estudios Avanzados (Mexico City, Mexico) animal facility. .. Antibodies and reagents used in this study are as follows: purified rabbit polyclonal immunoglobulin (Ig)G α-Myo1g , rabbit α-Myo1g (Santa Cruz Biotechnology, Dallas, TX, USA), goat α-Myo1g (Santa Cruz Biotechnology), rat monoclonal antibodies (mAb) NIM-R1 (α-Thy-1) and NIM-R8 (α-CD44) , biotin-labeled mAb α-CD44 (clone IM7) (BD Pharmingen, San Diego, CA, USA), α-rat-PE (BD Pharmingen), purified α-goat-PE, α-rabbit IgG-Alexa488 (Molecular Probes, Eugene, OR, USA), α-rabbit IgG-FITC (Jackson ImmunoResearch, West Grove, PA, USA), α-rabbit IgG-Cy3 (Jackson ImmunoResearch), Streptavidin-Cy3 (Invitrogen, Frederick, MD, USA), TRITC-phalloidin (Molecular Probes), FITC-phalloidin (Sigma Chemical Co, St. Louis, MO, USA), Streptavidin-APC (BD), Pacific Blue α-B220 (BD Pharmingen) α-IgM (Zymed Laboratories, San Francisco, CA, USA), α-Rab5 (Santa Cruz Biotechnology), α-CDC42 (Santa Cruz Biotechnology), α-CDC42-GTP (New East Biosciences, Brownsville, TX, USA), α-RhoA (Santa Cruz Biotechnology), α-RhoA-GTP (New East Biosciences), α-Actin (Santa Cruz Biotechnology), mAb α-CD44 (clone KM201, Abcam Cambridge, UK), α-Ezrin (Santa Cruz Biotechnology), α-Caveolin (Santa Cruz Biotechnology), purified goat polyclonal IgG α-Rab11 , biotinylated cholera toxin subunit B (Life Technologies, Grand Island, NY), Streptavidin-FITC (Sigma), α-CD44-PE (BioLegend, San Diego, CA, USA), and α-B220-VB421 (BioLegend), Primaquine (Sigma), Lanosterol (Sigma), Cholesterol (Sigma), Golgi-stop (Becton Dickinson, East Rutherford, NJ, USA).

Gas Chromatography-Mass Spectrometry:

Article Title: Sea cucumber genome provides insights into saponin biosynthesis and aestivation regulation
Article Snippet: Lanosterol (Cat# L5768) was purchased from Sigma-Aldrich, whereas parkeol and lanostadienol were purified and characterized according to published literatures , . .. The standards were dissolved and diluted to 0.5 mg/ml in hexane and used in GC-MS. Ketoconazole (Catlog # K1003) was purchased from Sigma dissolved in DMSO and used in yeast media.

Article Title: Producing aglycons of ginsenosides in bakers' yeast
Article Snippet: The samples were then centrifuged at 10,000×g for 1 min, and 1 μl of supernatant was analysed by GC/MS using an Agilent technologies 5975C insert xl MSD with triple-Axis Detector equipped with a HP-5ms (30 m×0.25 mm×0.5 μm) GC column. .. A crystallised β-amyrin sample was used as the standard for quantification (purchased from BioBioPha, China), and squalene, lanosterol and ergosterol standards (purchased from Sigma Aldrich) were also used for quantification.

Thin Layer Chromatography:

Article Title: Pneumocysterol [(24Z)-ethylidenelanost-8-en-3?-ol], a rare sterol detected in the opportunistic pathogen Pneumocystis carinii hominis: Structural identity and chemical synthesis
Article Snippet: Procedures and conditions for obtaining melting points, IR spectra, 1 H-NMR spectra, MS, TLC, GLC, and column chromatography were as described ( ). .. Commercial lanosterol (Sigma) was purified by using multiple ( ) recrystallization from acetone/water, and after recrystallization was found to be a mixture of lanosterol (Fig. , compound I ) and 24,25-dihydrolanosterol.

Article Title: Interfacial Behavior of Cholesterol, Ergosterol, and Lanosterol in Mixtures with DPPC and DMPC
Article Snippet: 1-Palmitoyl-2-myristoyl- sn -glycero-3-phosphocholine (DMPC), β -cholesterol, lanosterol and NaCl were purchased from Sigma (St. Louis, MO), 1,2-dipalmitoyl- sn -glycero-3-phosphocholine (DPPC) from Avanti Polar Lipids (Alabaster, AL), and ergosterol from Fluka (Neu-Ulm, Germany). .. The purity of the above lipids was verified by thin layer chromatography on silicic acid-coated plates (Merck, Darmstadt, Germany), using chloroform/methanol/water/ammonia (65:20:2:2, v/v) as the eluent.

Liquid Chromatography with Mass Spectroscopy:

Article Title: Producing aglycons of ginsenosides in bakers' yeast
Article Snippet: A crystallised β-amyrin sample was used as the standard for quantification (purchased from BioBioPha, China), and squalene, lanosterol and ergosterol standards (purchased from Sigma Aldrich) were also used for quantification. .. For the determination of oleanolic acid, acetone and methanol (1:1) extracts (20 μl) were analysed by LC/MS using an Agilent 1200 HPLC system coupled to a Bruker-micrOTOF-II with an electrospray ionisation (ESI) interface.

Staining:

Article Title: Interfacial Behavior of Cholesterol, Ergosterol, and Lanosterol in Mixtures with DPPC and DMPC
Article Snippet: 1-Palmitoyl-2-myristoyl- sn -glycero-3-phosphocholine (DMPC), β -cholesterol, lanosterol and NaCl were purchased from Sigma (St. Louis, MO), 1,2-dipalmitoyl- sn -glycero-3-phosphocholine (DPPC) from Avanti Polar Lipids (Alabaster, AL), and ergosterol from Fluka (Neu-Ulm, Germany). .. No impurities were detected upon examination of the plates after iodine staining.

other:

Article Title: Divergent interactions involving the oxidosqualene cyclase and the steroid-3-ketoreductase in the sterol biosynthetic pathway of mammals and yeasts
Article Snippet: Reagents, culture media, lanosterol, 5α-cholestan-3-one, cholesterol, ergosterol and bovine albumin were purchased from Sigma-Aldrich (Milan, Italy).

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  • 99
    Millipore lcs 1
    SOD1 is necessary for oncogene-driven proliferation of primary mouse epithelial cells. (a) Immunoblotting of MCL1-s, p53, and p16 of SOD1 +/+, SOD1 +/−, and SOD1 −/− iErBb2 mammary gland. (b) Quantification of MCL1-s, p53, and p16 expression from (a) relative to loading. (c) Representative images of immunohistochemistry of p16 in SOD +/+, SOD +/−, and SOD −/− iErbB2 mammary glands. (d) Quantification of percentage of positive p16 stain from (c). (e) Brightfield images of 3D primary mammary epithelial organoids from SOD +/+ and SOD +/− iErbB2 mice in the presence doxycycline at Day 1 (n=5), 4 (n=5), and 7 (n=5). Scale bar represents 100 uM. (f) Measurements of diameter of 3D organoids from (e). (g) Percentage of organoids from (e) with ErbB2 driven morphogenesis (n=3, 10 observations per time point). (h) Immunofluorescence of MCL-1 (green) in SOD +/+ and SOD +/− erbB2 organoids. Nuclei were stained with DAPI. (i) Representative images of Senescence-Associated-β-galactosidase (SA-β-gal) staining of SOD +/+ and SOD +/− iErbB2 mammary epithelial cells. (j) Percentage of SA-β-gal positive cells. (k) Representative images of colony formation assay or iErb2 tumor cells treated with DMSO (Control) or SOD1 inhibitor <t>LCS-1</t> (n = 5). (l) Quantification of colony formation assay in k. (m) MTT Proliferation assay of iErb2 tumor cells treated with DMSO (Control) or LCS-1 (n = 3).
    Lcs 1, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lcs 1/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lcs 1 - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    97
    Millipore hplc column
    <t>HPLC</t> purification of BChE The 64% pure BChE recovered from the affinity column was desalted to about 0.04 M NaCl, reduced in volume, and filtered into a 25 ml sample loop in preparation for loading onto the <t>Protein-Pak</t> DEAE column. About 600 ml of 11
    Hplc Column, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc column/product/Millipore
    Average 97 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    hplc column - by Bioz Stars, 2020-04
    97/100 stars
      Buy from Supplier

    87
    Millipore uv hplc
    Growth parameters of the S. spinosa CCTCC M206084 fermentation culture. The growth curve (red line) was drawn according to the OD 600 values, whereas the <t>spinosad</t> yield curve (green line) and glucose consumption curve (brown line) were drawn according to the analysis by <t>HPLC,</t> and the pH value (blue line) were taken to monitor growth every 24 h. Samples for proteomic analysis were collected at 2, 4, 8, and 12 d post-inoculation from fermentation cultures, as T1, T2, T3, and T4 phases, respectively. The logarithmic phase was from 0 d to T2, the stationary phase was from T2 to T3, whereas the decline phase was from T3 to T4.
    Uv Hplc, supplied by Millipore, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/uv hplc/product/Millipore
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    uv hplc - by Bioz Stars, 2020-04
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    86
    Millipore hilic hplc fractions
    PLS-DA analysis of tumor versus control using the <t>HILIC-HPLC</t> data. A , PLS-DA score plot of tumor samples ( red squares ) and control samples ( blue dots ) after OSC. B , demonstration of the validity of the model using a 200-permutation test of the six components. Intercepts: R 2 = 0.77 ( green triangles ) and Q 2 = −0.69 ( blue squares ).
    Hilic Hplc Fractions, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    SOD1 is necessary for oncogene-driven proliferation of primary mouse epithelial cells. (a) Immunoblotting of MCL1-s, p53, and p16 of SOD1 +/+, SOD1 +/−, and SOD1 −/− iErBb2 mammary gland. (b) Quantification of MCL1-s, p53, and p16 expression from (a) relative to loading. (c) Representative images of immunohistochemistry of p16 in SOD +/+, SOD +/−, and SOD −/− iErbB2 mammary glands. (d) Quantification of percentage of positive p16 stain from (c). (e) Brightfield images of 3D primary mammary epithelial organoids from SOD +/+ and SOD +/− iErbB2 mice in the presence doxycycline at Day 1 (n=5), 4 (n=5), and 7 (n=5). Scale bar represents 100 uM. (f) Measurements of diameter of 3D organoids from (e). (g) Percentage of organoids from (e) with ErbB2 driven morphogenesis (n=3, 10 observations per time point). (h) Immunofluorescence of MCL-1 (green) in SOD +/+ and SOD +/− erbB2 organoids. Nuclei were stained with DAPI. (i) Representative images of Senescence-Associated-β-galactosidase (SA-β-gal) staining of SOD +/+ and SOD +/− iErbB2 mammary epithelial cells. (j) Percentage of SA-β-gal positive cells. (k) Representative images of colony formation assay or iErb2 tumor cells treated with DMSO (Control) or SOD1 inhibitor LCS-1 (n = 5). (l) Quantification of colony formation assay in k. (m) MTT Proliferation assay of iErb2 tumor cells treated with DMSO (Control) or LCS-1 (n = 3).

    Journal: Oncogene

    Article Title: SOD1 is essential for oncogene-driven mammary tumor formation but dispensable for normal development and proliferation.

    doi: 10.1038/s41388-019-0839-x

    Figure Lengend Snippet: SOD1 is necessary for oncogene-driven proliferation of primary mouse epithelial cells. (a) Immunoblotting of MCL1-s, p53, and p16 of SOD1 +/+, SOD1 +/−, and SOD1 −/− iErBb2 mammary gland. (b) Quantification of MCL1-s, p53, and p16 expression from (a) relative to loading. (c) Representative images of immunohistochemistry of p16 in SOD +/+, SOD +/−, and SOD −/− iErbB2 mammary glands. (d) Quantification of percentage of positive p16 stain from (c). (e) Brightfield images of 3D primary mammary epithelial organoids from SOD +/+ and SOD +/− iErbB2 mice in the presence doxycycline at Day 1 (n=5), 4 (n=5), and 7 (n=5). Scale bar represents 100 uM. (f) Measurements of diameter of 3D organoids from (e). (g) Percentage of organoids from (e) with ErbB2 driven morphogenesis (n=3, 10 observations per time point). (h) Immunofluorescence of MCL-1 (green) in SOD +/+ and SOD +/− erbB2 organoids. Nuclei were stained with DAPI. (i) Representative images of Senescence-Associated-β-galactosidase (SA-β-gal) staining of SOD +/+ and SOD +/− iErbB2 mammary epithelial cells. (j) Percentage of SA-β-gal positive cells. (k) Representative images of colony formation assay or iErb2 tumor cells treated with DMSO (Control) or SOD1 inhibitor LCS-1 (n = 5). (l) Quantification of colony formation assay in k. (m) MTT Proliferation assay of iErb2 tumor cells treated with DMSO (Control) or LCS-1 (n = 3).

    Article Snippet: Cells were treated with either dimethyl sufoxide (Sigma Aldrich) or 3 μM LCS-1 (EMB Millipore, San Diego, CA, USA, 567417).

    Techniques: Expressing, Immunohistochemistry, Staining, Mouse Assay, Immunofluorescence, Colony Assay, MTT Assay, Proliferation Assay

    HPLC purification of BChE The 64% pure BChE recovered from the affinity column was desalted to about 0.04 M NaCl, reduced in volume, and filtered into a 25 ml sample loop in preparation for loading onto the Protein-Pak DEAE column. About 600 ml of 11

    Journal:

    Article Title: LARGE SCALE PURIFICATION OF BUTYRYLCHOLINESTERASE FROM HUMAN PLASMA SUITABLE FOR INJECTION INTO MONKEYS; A POTENTIAL NEW THERAPEUTIC FOR PROTECTION AGAINST COCAINE AND NERVE AGENT TOXICITY

    doi: 10.1901/jaba.2005.3-nihms5095

    Figure Lengend Snippet: HPLC purification of BChE The 64% pure BChE recovered from the affinity column was desalted to about 0.04 M NaCl, reduced in volume, and filtered into a 25 ml sample loop in preparation for loading onto the Protein-Pak DEAE column. About 600 ml of 11

    Article Snippet: The binding capacity of the 7.8 ml HPLC column (Protein-Pak DEAE, 10×100 mm, Millipore) is very high.

    Techniques: High Performance Liquid Chromatography, Purification, Affinity Column

    Growth parameters of the S. spinosa CCTCC M206084 fermentation culture. The growth curve (red line) was drawn according to the OD 600 values, whereas the spinosad yield curve (green line) and glucose consumption curve (brown line) were drawn according to the analysis by HPLC, and the pH value (blue line) were taken to monitor growth every 24 h. Samples for proteomic analysis were collected at 2, 4, 8, and 12 d post-inoculation from fermentation cultures, as T1, T2, T3, and T4 phases, respectively. The logarithmic phase was from 0 d to T2, the stationary phase was from T2 to T3, whereas the decline phase was from T3 to T4.

    Journal: Microbial Cell Factories

    Article Title: Differential proteomic profiling reveals regulatory proteins and novel links between primary metabolism and spinosad production in Saccharopolyspora spinosa

    doi: 10.1186/1475-2859-13-27

    Figure Lengend Snippet: Growth parameters of the S. spinosa CCTCC M206084 fermentation culture. The growth curve (red line) was drawn according to the OD 600 values, whereas the spinosad yield curve (green line) and glucose consumption curve (brown line) were drawn according to the analysis by HPLC, and the pH value (blue line) were taken to monitor growth every 24 h. Samples for proteomic analysis were collected at 2, 4, 8, and 12 d post-inoculation from fermentation cultures, as T1, T2, T3, and T4 phases, respectively. The logarithmic phase was from 0 d to T2, the stationary phase was from T2 to T3, whereas the decline phase was from T3 to T4.

    Article Snippet: The quantification of spinosad by UV-HPLC was performed as previously described with slight modification [ ], spinosad was extracted from the suspension culture by incubating the cell suspension with acetone at a 1:1 volumetric ratio for 48 h. Cultures were centrifuged at 9000 rpm for 15 min, and the supernatants were filtered through 0.22 μm Millipore filters.

    Techniques: High Performance Liquid Chromatography

    PLS-DA analysis of tumor versus control using the HILIC-HPLC data. A , PLS-DA score plot of tumor samples ( red squares ) and control samples ( blue dots ) after OSC. B , demonstration of the validity of the model using a 200-permutation test of the six components. Intercepts: R 2 = 0.77 ( green triangles ) and Q 2 = −0.69 ( blue squares ).

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: N-glycosylation of Colorectal Cancer Tissues

    doi: 10.1074/mcp.M111.011601

    Figure Lengend Snippet: PLS-DA analysis of tumor versus control using the HILIC-HPLC data. A , PLS-DA score plot of tumor samples ( red squares ) and control samples ( blue dots ) after OSC. B , demonstration of the validity of the model using a 200-permutation test of the six components. Intercepts: R 2 = 0.77 ( green triangles ) and Q 2 = −0.69 ( blue squares ).

    Article Snippet: Five μl of dried and reconstituted HILIC-HPLC fractions were desalted using a C18 ZipTipTM (Millipore, Billerica, MA) following the manufacturer's instructions.

    Techniques: Hydrophilic Interaction Liquid Chromatography, High Performance Liquid Chromatography

    Demonstration of the major discriminators from the PLS-DA analysis of HILIC-HPLC data. A , loading plot of the PLS-DA model classifying tumor and control tissues. The data point numbers are indicated. B , aligned HILIC chromatograms of tumor ( red ) and control tissues ( blue ). The chromatographic regions that contribute to the differentiation between tumor and control are indicated in A and B with a–d (elevated in tumor) and e and f (elevated in control). Compositions of the major glycans are given for the chromatographic peaks that discriminate between tumor and control.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: N-glycosylation of Colorectal Cancer Tissues

    doi: 10.1074/mcp.M111.011601

    Figure Lengend Snippet: Demonstration of the major discriminators from the PLS-DA analysis of HILIC-HPLC data. A , loading plot of the PLS-DA model classifying tumor and control tissues. The data point numbers are indicated. B , aligned HILIC chromatograms of tumor ( red ) and control tissues ( blue ). The chromatographic regions that contribute to the differentiation between tumor and control are indicated in A and B with a–d (elevated in tumor) and e and f (elevated in control). Compositions of the major glycans are given for the chromatographic peaks that discriminate between tumor and control.

    Article Snippet: Five μl of dried and reconstituted HILIC-HPLC fractions were desalted using a C18 ZipTipTM (Millipore, Billerica, MA) following the manufacturer's instructions.

    Techniques: Hydrophilic Interaction Liquid Chromatography, High Performance Liquid Chromatography