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Agilent technologies hplc
Hplc, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 95/100, based on 2692 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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High Performance Liquid Chromatography:

Article Title: Structural characterization and hypolipidemic activities of purified stigma maydis polysaccharides. Structural characterization and hypolipidemic activities of purified stigma maydis polysaccharides
Article Snippet: .. The samples were analyzed using HPLC equipped with an Agilent 1260 HPLC system, an Agilent Eclipse XDB‐C18 column (150 mm × 4.6 mm, 5 μm), and a DAD detector (254 nm). ..

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Article Title: Modified substrate specificity of a methyltransferase domain by protein insertion into an adenylation domain of the bassianolide synthetase
Article Snippet: Mass spectra of the compounds were collected by the HPLC coupled with an Agilent 6130 Single Quad mass spectrometer.

Article Title: An Experimental-Numerical Investigation on the Effects of Macroporous Scaffold Geometry on Cell Culture Parameters
Article Snippet: Glucose and Lactate Concentration Assay via HPLC The exhausted culture medium samples arising both from the perfusion bioreactor and the static culture were analyzed by HPLC using an Agilent/HP1100 Series system equipped with degasser unit, quaternary pump, autosampler, column thermostatic compartment, Agilent 1362A diode array detector (DAD), Agilent 1315A refractive index detector (RID), ICSep ICE-ION-300 column, and ICSep ICE-ION-300 precolumn cartridge 2/pk by Transgenomic.

Article Title: Protic Ionic Liquids as Efficient Solvents in Microwave-Assisted Extraction of Rhein and Emodin from Rheum palmatum L.
Article Snippet: The HPLC conditions were as follows: column temperature, 30 °C; injection volume, 5.0 μL; detection wavelength, 254 nm; separation column, ZORBAX Eclipse XDB-C18 column (4.6 mm × 150 mm, 5 μm, Agilent Technologies, Santa Clara, USA); flow rate, 1.0 mL∙min−1 ; mobile phase, a mixture of acetonitrile and 0.1% (v /v ) acetic acid aqueous solution (40% (v /v ) acetonitrile); run time, 15 min.

Article Title: Biologically active metabolite(s) from haemolymph of red-headed centipede Scolopendra subspinipes possess broad spectrum antibacterial activity
Article Snippet: The mass spectra of the compounds retrieved from HPLC were run against Metlin_AM_PCDL-N-170502.cdb for identification with exact homology through Agilent Mass Hunter software, while keeping in view compensation needed for charges in positive ESI MS as well as electron fragmentations, to ensure searches for the correct parent mass.

Article Title: Discovery of Stealthin Derivatives and Implication of the Amidotransferase FlsN3 in the Biosynthesis of Nitrogen-Containing Fluostatins
Article Snippet: The analytical HPLC was performed on an Agilent 1260 Infinity series instrument (Agilent Technologies, Inc., Santa Clara, CA, USA) equipped with a diode array detector (DAD) using an Agilent ZORBAX SB-C18 column (150 mm × 4.6 mm, 5 μm) or a Chiral ND 5u (4.6 × 250 mm) chiral column (Phenomenex, Washington, CD, USA) and run the following program: 5% B to 80% B (linear gradient, 0–20 min), 80% B to 100% B (20–21 min), 100% B (isocratic elution, 21–24 min), 100% B to 5% B (24–25 min), 5% B (isocratic elution, 25–30 min); the solvent system comprises solvent A (10% acetonitrile in water supplemented with 0.08% formic acid) and B (90% acetonitrile in water).

Article Title: Phenolic Compounds from Belamcanda chinensis Seeds
Article Snippet: NMR spectra were recorded on an AV-400 (Bruker, Karlsruhe, Germany) or an AV-600 (Bruker, Karlsruhe, Germany) spectrometer with TMS as an internal standard.

Article Title: Insight into the Influence of Cultivar Type, Cultivation Year, and Site on the Lignans and Related Phenolic Profiles, and the Health-Promoting Antioxidant Potential of Flax (Linum usitatissimum L.) Seeds
Article Snippet: Extraction, HPLC, and LC-ESI-MS Analysis Extractions (4 biological and 2 technical replicates), quantification of compounds was carried out on a Varian liquid chromatographic system (Agilent Technology, Les Ulis, France), as well as LC-ESI-MS analyses using a Waters 2695 Alliance coupled with a single quadrupole mass spectrometer ZQ (Waters-Micromass, Manchester, UK), equipped with an electrospray ion source (ESI-MS), were performed as described in Corbin et al. (2015) [ ].

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  • 85
    Agilent technologies vtr hplc
    <t>HPLC</t> chromatograms of <t>VtR</t> and chemical structure of (+)-vitisin A ( 1 ), (−)-vitisin B ( 2 ), and ampelopsin C ( 3 ). Retention times for internal standard (4-hydroxy-3-methoxycinnamaldehyde, IS), ampelopsin C ( 3 ), (+)-vitisin A ( 1 ), and (−)-vitisin B ( 2 ) were 15.0, 21.1, 25.7, and 29.7 min, respectively. The traced component of peak 4 with a retention time of 17.9 min was resveratrol.
    Vtr Hplc, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Agilent technologies agilent 1200 hplc
    Performance comparison between EO-pumped <t>HPLC</t> and <t>Agilent</t> 1200 HPLC. (a) HPLC setup with high-pressure on-chip EO pump. L1 and L2: two loops on 10-port valve; E(i): eluent i; V: 10 nL injection valve; C: Waters Atlantis C18 column (75 μm i.d. and 100 mm length); D: Linear UVIS 200 absorbance detector (210 nm); and W: waste. Inset: the other position of the 10-port valve. 6 kV was applied across all pump channels. (b) Typical chromatograms for trypsin digests of BSA, TF, and human IgG from the EO-pumped HPLC. The eluent contained a constant 0.1% trifluoroacetic acid and varying amount of acetonitrile in water. The acetonitrile concentration was initially increased by 3% every 3 min until 56%, then increased by 2% every 2 min until 60%, and then remained at 60% until the completion of the run (see the gradient profile in the top panel). The elution pressure was about 70–80 bar, and the pump rate was ∼160 nL/min. (c) Results from Agilent 1200 HPLC. A flow splitter was used between the 10 nL injection valve and Agilent 1200 pump. The flow rate of the Agilent pump was set at 90 μL/min, resulting in an elution rate of ∼160 nL/min. The gradient stayed at 5% for the first 2 min, then went from 5% to 20% in 15 min, 20% to 40% in the next 30 min, 40% to 60% in the next 40 min, and stayed at 60% to complete the separation. All other conditions were the same as in (b).
    Agilent 1200 Hplc, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 687 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Agilent technologies 1100 capillary hplc ion trap ms system
    <t>HPLC-ESI</t> + -MS/MS analysis of oxazolone in enzymatic hydrolysates of rat liver DNA (300 μg) following off-line HPLC purification ( Scheme 2 ). An Agilent <t>1100</t> series capillary HPLC was interfaced to a Thermo-Finnigan TSQ Quantum Ultra mass spectrometer. A Thermo Hypersil-Keystone Hypercarb column (0.5 × 100 mm, 5 μm) was eluted at a flow rate of 12 μl/min with a gradient of isopropanol/acetonitrile (3:1) (solvent B) in 0.05% acetic acid (solvent A). The spray voltage was set to 3.1 kV, the source temperature was 250°C, and the sheath gas pressure was 30 psi. Quantitative analyses were performed in selected reaction monitoring mode using the transitions m/z 247.1→87.1 [M + 2H − dR − CO 2 ] + , and m/z 251.1→91.1 for oxazolone and 15 N 4 -oxazolone, respectively.
    1100 Capillary Hplc Ion Trap Ms System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Agilent technologies high performance liquid chromatography hplc ido 1 dioxygenase activity
    GGC does not abrogate LPS-induced inflammatory and immune regulatory responses. Culture media collected from the proteomics analyses from mock, LPS + , therapeutic (T), prophylactic (P), T+P and GGC only conditions were assayed for ( A ) IL-8 cytokine and ( B ) <t>IDO-1</t> activity measured as the kynurenine to tryptophan ratio. Statistical analysis was performed using two-way ANOVA against LPS + for T, P and T+P conditions, and mock for LPS + and GGC only (*** = p
    High Performance Liquid Chromatography Hplc Ido 1 Dioxygenase Activity, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    HPLC chromatograms of VtR and chemical structure of (+)-vitisin A ( 1 ), (−)-vitisin B ( 2 ), and ampelopsin C ( 3 ). Retention times for internal standard (4-hydroxy-3-methoxycinnamaldehyde, IS), ampelopsin C ( 3 ), (+)-vitisin A ( 1 ), and (−)-vitisin B ( 2 ) were 15.0, 21.1, 25.7, and 29.7 min, respectively. The traced component of peak 4 with a retention time of 17.9 min was resveratrol.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: A Special Ingredient (VtR) Containing Oligostilbenes Isolated from Vitis thunbergii Prevents Bone Loss in Ovariectomized Mice: In Vitro and In Vivo Study

    doi: 10.1155/2013/409421

    Figure Lengend Snippet: HPLC chromatograms of VtR and chemical structure of (+)-vitisin A ( 1 ), (−)-vitisin B ( 2 ), and ampelopsin C ( 3 ). Retention times for internal standard (4-hydroxy-3-methoxycinnamaldehyde, IS), ampelopsin C ( 3 ), (+)-vitisin A ( 1 ), and (−)-vitisin B ( 2 ) were 15.0, 21.1, 25.7, and 29.7 min, respectively. The traced component of peak 4 with a retention time of 17.9 min was resveratrol.

    Article Snippet: HPLC Analysis of VtR HPLC was conducted on an Agilent 1100 series equipped with a G1311A QuatPump, G1379A degasser, G1315B photodiode array detector, and a 1200 series G1329A ALS.

    Techniques: High Performance Liquid Chromatography

    Performance comparison between EO-pumped HPLC and Agilent 1200 HPLC. (a) HPLC setup with high-pressure on-chip EO pump. L1 and L2: two loops on 10-port valve; E(i): eluent i; V: 10 nL injection valve; C: Waters Atlantis C18 column (75 μm i.d. and 100 mm length); D: Linear UVIS 200 absorbance detector (210 nm); and W: waste. Inset: the other position of the 10-port valve. 6 kV was applied across all pump channels. (b) Typical chromatograms for trypsin digests of BSA, TF, and human IgG from the EO-pumped HPLC. The eluent contained a constant 0.1% trifluoroacetic acid and varying amount of acetonitrile in water. The acetonitrile concentration was initially increased by 3% every 3 min until 56%, then increased by 2% every 2 min until 60%, and then remained at 60% until the completion of the run (see the gradient profile in the top panel). The elution pressure was about 70–80 bar, and the pump rate was ∼160 nL/min. (c) Results from Agilent 1200 HPLC. A flow splitter was used between the 10 nL injection valve and Agilent 1200 pump. The flow rate of the Agilent pump was set at 90 μL/min, resulting in an elution rate of ∼160 nL/min. The gradient stayed at 5% for the first 2 min, then went from 5% to 20% in 15 min, 20% to 40% in the next 30 min, 40% to 60% in the next 40 min, and stayed at 60% to complete the separation. All other conditions were the same as in (b).

    Journal: Analytical Chemistry

    Article Title: High-Pressure Open-Channel On-Chip Electroosmotic Pump for Nanoflow High Performance Liquid Chromatography

    doi: 10.1021/ac4040345

    Figure Lengend Snippet: Performance comparison between EO-pumped HPLC and Agilent 1200 HPLC. (a) HPLC setup with high-pressure on-chip EO pump. L1 and L2: two loops on 10-port valve; E(i): eluent i; V: 10 nL injection valve; C: Waters Atlantis C18 column (75 μm i.d. and 100 mm length); D: Linear UVIS 200 absorbance detector (210 nm); and W: waste. Inset: the other position of the 10-port valve. 6 kV was applied across all pump channels. (b) Typical chromatograms for trypsin digests of BSA, TF, and human IgG from the EO-pumped HPLC. The eluent contained a constant 0.1% trifluoroacetic acid and varying amount of acetonitrile in water. The acetonitrile concentration was initially increased by 3% every 3 min until 56%, then increased by 2% every 2 min until 60%, and then remained at 60% until the completion of the run (see the gradient profile in the top panel). The elution pressure was about 70–80 bar, and the pump rate was ∼160 nL/min. (c) Results from Agilent 1200 HPLC. A flow splitter was used between the 10 nL injection valve and Agilent 1200 pump. The flow rate of the Agilent pump was set at 90 μL/min, resulting in an elution rate of ∼160 nL/min. The gradient stayed at 5% for the first 2 min, then went from 5% to 20% in 15 min, 20% to 40% in the next 30 min, 40% to 60% in the next 40 min, and stayed at 60% to complete the separation. All other conditions were the same as in (b).

    Article Snippet: Figure b,c presents a performance comparison between chromatograms from the EO-pumped HPLC (Figure b) and those from an Agilent 1200 HPLC (Figure c).

    Techniques: High Performance Liquid Chromatography, Chromatin Immunoprecipitation, Injection, Concentration Assay, Flow Cytometry

    HPLC-ESI + -MS/MS analysis of oxazolone in enzymatic hydrolysates of rat liver DNA (300 μg) following off-line HPLC purification ( Scheme 2 ). An Agilent 1100 series capillary HPLC was interfaced to a Thermo-Finnigan TSQ Quantum Ultra mass spectrometer. A Thermo Hypersil-Keystone Hypercarb column (0.5 × 100 mm, 5 μm) was eluted at a flow rate of 12 μl/min with a gradient of isopropanol/acetonitrile (3:1) (solvent B) in 0.05% acetic acid (solvent A). The spray voltage was set to 3.1 kV, the source temperature was 250°C, and the sheath gas pressure was 30 psi. Quantitative analyses were performed in selected reaction monitoring mode using the transitions m/z 247.1→87.1 [M + 2H − dR − CO 2 ] + , and m/z 251.1→91.1 for oxazolone and 15 N 4 -oxazolone, respectively.

    Journal: Nucleic Acids Research

    Article Title: Quantitative analysis of the oxidative DNA lesion, 2,2-diamino-4-(2-deoxy-?-d-erythro-pentofuranosyl)amino]-5(2H)-oxazolone (oxazolone), in vitro and in vivo by isotope dilution-capillary HPLC-ESI-MS/MS

    doi: 10.1093/nar/gkl596

    Figure Lengend Snippet: HPLC-ESI + -MS/MS analysis of oxazolone in enzymatic hydrolysates of rat liver DNA (300 μg) following off-line HPLC purification ( Scheme 2 ). An Agilent 1100 series capillary HPLC was interfaced to a Thermo-Finnigan TSQ Quantum Ultra mass spectrometer. A Thermo Hypersil-Keystone Hypercarb column (0.5 × 100 mm, 5 μm) was eluted at a flow rate of 12 μl/min with a gradient of isopropanol/acetonitrile (3:1) (solvent B) in 0.05% acetic acid (solvent A). The spray voltage was set to 3.1 kV, the source temperature was 250°C, and the sheath gas pressure was 30 psi. Quantitative analyses were performed in selected reaction monitoring mode using the transitions m/z 247.1→87.1 [M + 2H − dR − CO 2 ] + , and m/z 251.1→91.1 for oxazolone and 15 N 4 -oxazolone, respectively.

    Article Snippet: Our HPLC-ESI-MS/MS method for 8-oxo-dG employed selected reaction monitoring of the transitions 284.2 [M + H]+ →168.2 [M + 2H − dR]+ (8-oxo-dG) and 289.2 [M + H]+ →173.2 [M + 2H − dR]+ (15 N5 –8-oxo-dG) on an Agilent 1100 capillary HPLC- Ion Trap MS system.

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry, Purification, Flow Cytometry

    HPLC-ESI + -MS/MS analysis of 8-oxo-dG in an enzymatic hydrolysate of rat liver DNA (80 μg). An Agilent 1100 series capillary HPLC-ion trap MS system was used. A Zorbax SB C18 column (0.5 × 150 mm, 5 (m) was maintained at 10°C and eluted at a flow rate of 12 (l/min with a gradient of methanol (solvent B) in 15 mM ammonium acetate (solvent A). The mass spectrometer was operated in the positive ion MS/MS mode. Quantitative analyses were performed in selected reaction monitoring mode using the transitions m/z 284.1→168.0 (M + 2H − dR) + for 8-oxo-dG and the corresponding transition m/z 289.1→173 for [ 15 N 5 ]-8-oxo-dG.

    Journal: Nucleic Acids Research

    Article Title: Quantitative analysis of the oxidative DNA lesion, 2,2-diamino-4-(2-deoxy-?-d-erythro-pentofuranosyl)amino]-5(2H)-oxazolone (oxazolone), in vitro and in vivo by isotope dilution-capillary HPLC-ESI-MS/MS

    doi: 10.1093/nar/gkl596

    Figure Lengend Snippet: HPLC-ESI + -MS/MS analysis of 8-oxo-dG in an enzymatic hydrolysate of rat liver DNA (80 μg). An Agilent 1100 series capillary HPLC-ion trap MS system was used. A Zorbax SB C18 column (0.5 × 150 mm, 5 (m) was maintained at 10°C and eluted at a flow rate of 12 (l/min with a gradient of methanol (solvent B) in 15 mM ammonium acetate (solvent A). The mass spectrometer was operated in the positive ion MS/MS mode. Quantitative analyses were performed in selected reaction monitoring mode using the transitions m/z 284.1→168.0 (M + 2H − dR) + for 8-oxo-dG and the corresponding transition m/z 289.1→173 for [ 15 N 5 ]-8-oxo-dG.

    Article Snippet: Our HPLC-ESI-MS/MS method for 8-oxo-dG employed selected reaction monitoring of the transitions 284.2 [M + H]+ →168.2 [M + 2H − dR]+ (8-oxo-dG) and 289.2 [M + H]+ →173.2 [M + 2H − dR]+ (15 N5 –8-oxo-dG) on an Agilent 1100 capillary HPLC- Ion Trap MS system.

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry, Flow Cytometry

    GGC does not abrogate LPS-induced inflammatory and immune regulatory responses. Culture media collected from the proteomics analyses from mock, LPS + , therapeutic (T), prophylactic (P), T+P and GGC only conditions were assayed for ( A ) IL-8 cytokine and ( B ) IDO-1 activity measured as the kynurenine to tryptophan ratio. Statistical analysis was performed using two-way ANOVA against LPS + for T, P and T+P conditions, and mock for LPS + and GGC only (*** = p

    Journal: bioRxiv

    Article Title: Novel antioxidant therapy with the immediate precursor to glutathione, γ-glutamylcysteine (GGC), ameliorates LPS-induced cellular stress in an in vitro cystic fibrosis model

    doi: 10.1101/2020.05.27.119990

    Figure Lengend Snippet: GGC does not abrogate LPS-induced inflammatory and immune regulatory responses. Culture media collected from the proteomics analyses from mock, LPS + , therapeutic (T), prophylactic (P), T+P and GGC only conditions were assayed for ( A ) IL-8 cytokine and ( B ) IDO-1 activity measured as the kynurenine to tryptophan ratio. Statistical analysis was performed using two-way ANOVA against LPS + for T, P and T+P conditions, and mock for LPS + and GGC only (*** = p

    Article Snippet: High performance liquid chromatography (HPLC) IDO-1 dioxygenase activity was determined by measuring the extent of conversion of L-tryptophan (L-Trp) into kynurenine (Kyn) in the culture medium using an Agilent-1260 HPLC system.

    Techniques: Activity Assay