hplc system  (Thermo Fisher)


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    Structured Review

    Thermo Fisher hplc system
    <t>Verapamil</t> and its metabolites produced following incubation with human heart microsomes. <t>HPLC</t> chromatogram depicting nine metabolites detected following the incubation of verapamil with microsomes prepared from human heart ventricles. Structure allocation was established following peak collection and reanalysis by LC-MSMS according to Walles et al. assay. [26] Metabolites PR22, D702, norverapamil and a hydroxymetabolite (retention time 14 min) were present in sufficient amounts to allow quantification. The characterization of CYP450-dependency on the formation of four major verapamil metabolites by microsomes prepared from human heart samples is illustrated. Metabolites could not be detected in the absence of a NAPDH regenerating system or in the presence of pre-heated microsomes.
    Hplc System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 587 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Metabolic Activity and mRNA Levels of Human Cardiac CYP450s Involved in Drug Metabolism"

    Article Title: Metabolic Activity and mRNA Levels of Human Cardiac CYP450s Involved in Drug Metabolism

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0015666

    Verapamil and its metabolites produced following incubation with human heart microsomes. HPLC chromatogram depicting nine metabolites detected following the incubation of verapamil with microsomes prepared from human heart ventricles. Structure allocation was established following peak collection and reanalysis by LC-MSMS according to Walles et al. assay. [26] Metabolites PR22, D702, norverapamil and a hydroxymetabolite (retention time 14 min) were present in sufficient amounts to allow quantification. The characterization of CYP450-dependency on the formation of four major verapamil metabolites by microsomes prepared from human heart samples is illustrated. Metabolites could not be detected in the absence of a NAPDH regenerating system or in the presence of pre-heated microsomes.
    Figure Legend Snippet: Verapamil and its metabolites produced following incubation with human heart microsomes. HPLC chromatogram depicting nine metabolites detected following the incubation of verapamil with microsomes prepared from human heart ventricles. Structure allocation was established following peak collection and reanalysis by LC-MSMS according to Walles et al. assay. [26] Metabolites PR22, D702, norverapamil and a hydroxymetabolite (retention time 14 min) were present in sufficient amounts to allow quantification. The characterization of CYP450-dependency on the formation of four major verapamil metabolites by microsomes prepared from human heart samples is illustrated. Metabolites could not be detected in the absence of a NAPDH regenerating system or in the presence of pre-heated microsomes.

    Techniques Used: Produced, Incubation, High Performance Liquid Chromatography

    2) Product Images from "A physico-chemical assessment of the thermal stability of pneumococcal conjugate vaccine components"

    Article Title: A physico-chemical assessment of the thermal stability of pneumococcal conjugate vaccine components

    Journal: Human Vaccines & Immunotherapeutics

    doi: 10.4161/hv.29696

    HPLC-SEC chromatograms of pneumococcal-CRM 197 bulk conjugate stability samples. Serotypes 1, 4, 5, 6B, 9V, 14, 18C, 19F, and 23F (A to I) were stored at temperatures of -20, 4, 37, or 56°C for for 8 wk or exposed to repeated freeze-thawing (F/T). Conditions were as for Figure 1 .
    Figure Legend Snippet: HPLC-SEC chromatograms of pneumococcal-CRM 197 bulk conjugate stability samples. Serotypes 1, 4, 5, 6B, 9V, 14, 18C, 19F, and 23F (A to I) were stored at temperatures of -20, 4, 37, or 56°C for for 8 wk or exposed to repeated freeze-thawing (F/T). Conditions were as for Figure 1 .

    Techniques Used: High Performance Liquid Chromatography, Size-exclusion Chromatography

    HPLC-SEC chromatograms of pneumococcal-PD/TT/DT bulk conjugates and carrier proteins stability samples. Serotypes 1, 4, 5, 6B, 7F, 9V, 14, 18C, 19F, 23F and PD, TT, DT (A to M) were stored at temperatures of -20, 4, 37, or 56°C for 5 wk, as labeled, or exposed to repeated freeze-thawing (F/T). Samples were loaded onto a TSK5000 PWXL column and eluted in PBS, pH7.4 with a flow rate 0.3 ml/min.
    Figure Legend Snippet: HPLC-SEC chromatograms of pneumococcal-PD/TT/DT bulk conjugates and carrier proteins stability samples. Serotypes 1, 4, 5, 6B, 7F, 9V, 14, 18C, 19F, 23F and PD, TT, DT (A to M) were stored at temperatures of -20, 4, 37, or 56°C for 5 wk, as labeled, or exposed to repeated freeze-thawing (F/T). Samples were loaded onto a TSK5000 PWXL column and eluted in PBS, pH7.4 with a flow rate 0.3 ml/min.

    Techniques Used: High Performance Liquid Chromatography, Size-exclusion Chromatography, Labeling, Flow Cytometry

    3) Product Images from "Response of Vibrio cholerae to the Catecholamine Hormones Epinephrine and Norepinephrine"

    Article Title: Response of Vibrio cholerae to the Catecholamine Hormones Epinephrine and Norepinephrine

    Journal: Journal of Bacteriology

    doi: 10.1128/JB.00345-15

    Detection of epinephrine and norepinephrine in serum-SAPI in the absence (A) or presence (B) of V. cholerae . Catecholates were analyzed by HPLC combined with electrochemical detection. (A) Supernatants from serum-SAPI without bacteria after 0 h or 48
    Figure Legend Snippet: Detection of epinephrine and norepinephrine in serum-SAPI in the absence (A) or presence (B) of V. cholerae . Catecholates were analyzed by HPLC combined with electrochemical detection. (A) Supernatants from serum-SAPI without bacteria after 0 h or 48

    Techniques Used: High Performance Liquid Chromatography

    4) Product Images from "Selenium species-dependent toxicity, bioavailability and metabolic transformations in Caenorhabditis elegans"

    Article Title: Selenium species-dependent toxicity, bioavailability and metabolic transformations in Caenorhabditis elegans

    Journal: Metallomics : integrated biometal science

    doi: 10.1039/c8mt00066b

    HPLC-ESI-Orbitrap-MS (background subtracted) of a C. elegans lysate following acute treatment (30 min) at L1 stage with SeMet: Identification of AdoSeHcy (species 13 ) via its isotope pattern and accurate mass; (A) calculated isotope pattern of AdoSeHcy, (B) spectrum of AdoSeHcy in the crude lysate (absolute intensity of the most intense peak in the isotope pattern of the species: 1.2 × 10 4 ). Due to their low absolute intensities (ca. 5 × 10 1 to 1 × 10 3 ; injection volume 20 μL.
    Figure Legend Snippet: HPLC-ESI-Orbitrap-MS (background subtracted) of a C. elegans lysate following acute treatment (30 min) at L1 stage with SeMet: Identification of AdoSeHcy (species 13 ) via its isotope pattern and accurate mass; (A) calculated isotope pattern of AdoSeHcy, (B) spectrum of AdoSeHcy in the crude lysate (absolute intensity of the most intense peak in the isotope pattern of the species: 1.2 × 10 4 ). Due to their low absolute intensities (ca. 5 × 10 1 to 1 × 10 3 ; injection volume 20 μL.

    Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry, Injection

    HPLC-ESI-Orbitrap-MS (background subtracted) of a C. elegans lysate following acute treatment (30 min) at L1 stage with SeMet: Identification of AdoSeMet (species 8 ) via its isotope pattern, accurate mass and fragmentation; (A) calculated isotope pattern of AdoSeMet, (B) spectrum of AdoSeMet in the crude lysate (absolute intensity of the most intense peak in the isotope pattern of the species: 5.4 × 105), (C) fragmentation of m/z ; injection volume 20 μL.
    Figure Legend Snippet: HPLC-ESI-Orbitrap-MS (background subtracted) of a C. elegans lysate following acute treatment (30 min) at L1 stage with SeMet: Identification of AdoSeMet (species 8 ) via its isotope pattern, accurate mass and fragmentation; (A) calculated isotope pattern of AdoSeMet, (B) spectrum of AdoSeMet in the crude lysate (absolute intensity of the most intense peak in the isotope pattern of the species: 5.4 × 105), (C) fragmentation of m/z ; injection volume 20 μL.

    Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry, Injection

    5) Product Images from "N-alkylamide profiling of Achillea ptarmica and Achillea millefolium extracts by liquid and gas chromatography–mass spectrometry"

    Article Title: N-alkylamide profiling of Achillea ptarmica and Achillea millefolium extracts by liquid and gas chromatography–mass spectrometry

    Journal: Journal of Pharmaceutical Analysis

    doi: 10.1016/j.jpha.2016.09.005

    TIC of Achillea millefolium obtained using HPLC–ESI–MS.
    Figure Legend Snippet: TIC of Achillea millefolium obtained using HPLC–ESI–MS.

    Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry

    TIC of Achillea ptarmica obtained using HPLC–ESI–MS.
    Figure Legend Snippet: TIC of Achillea ptarmica obtained using HPLC–ESI–MS.

    Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry

    6) Product Images from "Identification and Analysis of Amygdalin, Neoamygdalin and Amygdalin Amide in Different Processed Bitter Almonds by HPLC-ESI-MS/MS and HPLC-DAD"

    Article Title: Identification and Analysis of Amygdalin, Neoamygdalin and Amygdalin Amide in Different Processed Bitter Almonds by HPLC-ESI-MS/MS and HPLC-DAD

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    doi: 10.3390/molecules22091425

    Total ion chromatogram of amygdalins characterized in bitter almonds by HPLC-ESI-MS/MS. ( 1 ) amygdalin amide; ( 2 ) amygdalin; ( 3 ) neoamygdalin.
    Figure Legend Snippet: Total ion chromatogram of amygdalins characterized in bitter almonds by HPLC-ESI-MS/MS. ( 1 ) amygdalin amide; ( 2 ) amygdalin; ( 3 ) neoamygdalin.

    Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry

    7) Product Images from "Quantitative HPLC-MS analysis of nucleotide sugars in plant cells following off-line SPE sample preparation"

    Article Title: Quantitative HPLC-MS analysis of nucleotide sugars in plant cells following off-line SPE sample preparation

    Journal: Analytical and Bioanalytical Chemistry

    doi: 10.1007/s00216-014-7746-3

    Influence of the potential applied to the ESI source on retention of UDP-sugars. Chromatogram obtained with a newly installed or regenerated PGC column ( a ); chromatogram obtained after 20-min utilization of the column connected to the ESI source of an Orbitrap mass spectrometer ( b ). Conditions: column regeneration was performed with 80 % acetonitrile in water containing 0.10 % trifluoroacetic acid for 20 min, the sample measurements were done with the gradient program described in the materials and methods section for HPLC-MS; detection, UV, 262 nm; sample. UDP-Gal (100 μmol L −1 ) and UDP-Glc (100 μmol L −1 )
    Figure Legend Snippet: Influence of the potential applied to the ESI source on retention of UDP-sugars. Chromatogram obtained with a newly installed or regenerated PGC column ( a ); chromatogram obtained after 20-min utilization of the column connected to the ESI source of an Orbitrap mass spectrometer ( b ). Conditions: column regeneration was performed with 80 % acetonitrile in water containing 0.10 % trifluoroacetic acid for 20 min, the sample measurements were done with the gradient program described in the materials and methods section for HPLC-MS; detection, UV, 262 nm; sample. UDP-Gal (100 μmol L −1 ) and UDP-Glc (100 μmol L −1 )

    Techniques Used: Pyrolysis Gas Chromatography, Mass Spectrometry, High Performance Liquid Chromatography, Gas Chromatography

    Influence of the potential applied to the ESI source on retention of UDP-sugars. Chromatogram obtained with a newly installed or regenerated PGC column ( a ); chromatogram obtained after 20-min utilization of the column connected to the ESI source of an Orbitrap mass spectrometer ( b ). Conditions: column regeneration was performed with 80 % acetonitrile in water containing 0.10 % trifluoroacetic acid for 20 min, the sample measurements were done with the gradient program described in the materials and methods section for HPLC-MS; detection, UV, 262 nm; sample. UDP-Gal (100 μmol L −1 ) and UDP-Glc (100 μmol L −1 )
    Figure Legend Snippet: Influence of the potential applied to the ESI source on retention of UDP-sugars. Chromatogram obtained with a newly installed or regenerated PGC column ( a ); chromatogram obtained after 20-min utilization of the column connected to the ESI source of an Orbitrap mass spectrometer ( b ). Conditions: column regeneration was performed with 80 % acetonitrile in water containing 0.10 % trifluoroacetic acid for 20 min, the sample measurements were done with the gradient program described in the materials and methods section for HPLC-MS; detection, UV, 262 nm; sample. UDP-Gal (100 μmol L −1 ) and UDP-Glc (100 μmol L −1 )

    Techniques Used: Pyrolysis Gas Chromatography, Mass Spectrometry, High Performance Liquid Chromatography, Gas Chromatography

    Analyte recoveries after liquid-liquid extraction ( a ) and solid-phase extraction ( b ). Recoveries were calculated from triplicate HPLC analysis of three to five replicate extractions of 10 μmol L −1 standard solution of the respective UDP-sugars. The diagram shows arithmetic averages and relative standard deviations of all analyses performed; sample size in ( a ): N = 9 for UDP-Glc, UDP-Xyl, UDP-GalA, and UDP-GlcA, N = 15 for UDP-Ara and UDP-Gal, sample size in ( b ): N = 31 for UDP-Glc, N = 15 for UDP-Ara, UDP-Xyl, UDP-Gal, UDP-GlcA, UDP-GalA
    Figure Legend Snippet: Analyte recoveries after liquid-liquid extraction ( a ) and solid-phase extraction ( b ). Recoveries were calculated from triplicate HPLC analysis of three to five replicate extractions of 10 μmol L −1 standard solution of the respective UDP-sugars. The diagram shows arithmetic averages and relative standard deviations of all analyses performed; sample size in ( a ): N = 9 for UDP-Glc, UDP-Xyl, UDP-GalA, and UDP-GlcA, N = 15 for UDP-Ara and UDP-Gal, sample size in ( b ): N = 31 for UDP-Glc, N = 15 for UDP-Ara, UDP-Xyl, UDP-Gal, UDP-GlcA, UDP-GalA

    Techniques Used: High Performance Liquid Chromatography, Gas Chromatography, Acetylene Reduction Assay

    8) Product Images from "Genetic and isotope ratio mass spectrometric evidence for the occurrence of starch degradation and cycling in illuminated Arabidopsis leaves"

    Article Title: Genetic and isotope ratio mass spectrometric evidence for the occurrence of starch degradation and cycling in illuminated Arabidopsis leaves

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0171245

    HPLC/IRMS analyses of δ13 C in starch in leaves of 13 CO2 pulse-chased plants
    Figure Legend Snippet: HPLC/IRMS analyses of δ13 C in starch in leaves of 13 CO2 pulse-chased plants

    Techniques Used: High Performance Liquid Chromatography

    9) Product Images from "Deciphering the Cryptic Genome: Genome-wide Analyses of the Rice Pathogen Fusarium fujikuroi Reveal Complex Regulation of Secondary Metabolism and Novel Metabolites"

    Article Title: Deciphering the Cryptic Genome: Genome-wide Analyses of the Rice Pathogen Fusarium fujikuroi Reveal Complex Regulation of Secondary Metabolism and Novel Metabolites

    Journal: PLoS Pathogens

    doi: 10.1371/journal.ppat.1003475

    Functional characterization of the PKS19 cluster, a putative polyketide biosynthetic gene cluster that is unique to F. fujikuroi . (A) Position and organization of the PKS19 gene cluster on F. fujikuroi chromosome VIII, GC content, distribution of active histone marks, and gene expression in the PKS19 cluster. Histone marks are described in the legend for Figure 3 . Expression data are from microarray analysis of wild-type F. fujikuroi (strain IMI58289). Values are log 2 change in expression in a high versus low-nitrogen medium. H3K9ac and gene expression are correlated, as both are increased in the high-nitrogen medium. Some genes exhibited increased levels of H3K4me2 in the high-nitrogen medium, which also suggests transcription. (B) Chemical analysis of the SM product(s) of the PKS19 cluster. The traces show the combined extracted ion chromatograms for metabolites with molecular formulas [C 12 H 16 O 4 +H] + , [C 12 H 18 O 5 +H] + and [C 12 H 18 O 4 +H] + determined by HPLC-FTMS of culture fluids from F. fujikuroi strains: IMI58289, wild-type strain; OE::TF, a strain over-expressing the transcription factor encoded by FFUJ_12242; OE::PKS19, a strain over-expressing the PKS19 gene FFUJ_12239; and OE::TF/OE::PKS19, a strain over-expressing both FFUJ-12242 and FFUJ_12239. (C) Northern blot analysis of PKS19 cluster genes in strains IMI58289 (WT), OE::TF, OE::PKS19 and OE::TF/OE::PKS19. (D) UV spectra of metabolites corresponding to peaks 1 through 4 from chromatograms shown in C. The similar spectra of the metabolites suggest structural similarity.
    Figure Legend Snippet: Functional characterization of the PKS19 cluster, a putative polyketide biosynthetic gene cluster that is unique to F. fujikuroi . (A) Position and organization of the PKS19 gene cluster on F. fujikuroi chromosome VIII, GC content, distribution of active histone marks, and gene expression in the PKS19 cluster. Histone marks are described in the legend for Figure 3 . Expression data are from microarray analysis of wild-type F. fujikuroi (strain IMI58289). Values are log 2 change in expression in a high versus low-nitrogen medium. H3K9ac and gene expression are correlated, as both are increased in the high-nitrogen medium. Some genes exhibited increased levels of H3K4me2 in the high-nitrogen medium, which also suggests transcription. (B) Chemical analysis of the SM product(s) of the PKS19 cluster. The traces show the combined extracted ion chromatograms for metabolites with molecular formulas [C 12 H 16 O 4 +H] + , [C 12 H 18 O 5 +H] + and [C 12 H 18 O 4 +H] + determined by HPLC-FTMS of culture fluids from F. fujikuroi strains: IMI58289, wild-type strain; OE::TF, a strain over-expressing the transcription factor encoded by FFUJ_12242; OE::PKS19, a strain over-expressing the PKS19 gene FFUJ_12239; and OE::TF/OE::PKS19, a strain over-expressing both FFUJ-12242 and FFUJ_12239. (C) Northern blot analysis of PKS19 cluster genes in strains IMI58289 (WT), OE::TF, OE::PKS19 and OE::TF/OE::PKS19. (D) UV spectra of metabolites corresponding to peaks 1 through 4 from chromatograms shown in C. The similar spectra of the metabolites suggest structural similarity.

    Techniques Used: Functional Assay, Expressing, Microarray, High Performance Liquid Chromatography, Northern Blot

    Location of the NRPS/APS biosynthetic gene cluster on F. fujikuroi chromosome I, levels of histone modifications and gene expression within and flanking the cluster, and production of metabolites following overexpression of cluster genes APS2 and APS8 . A : Synteny between the apicidin gene cluster in F. semitectum [105] and the apicidin-like gene cluster in F. fujikuroi . B : Histone modifications and gene expression in and flanking the cluster. Histone marks are described in the legend to Figure 3 . Expression data were derived from microarray experiments in low and high nitrogen and are plotted as the changes in log 2 expression values in high-nitrogen medium compared to low-nitrogen medium. H3K9ac and gene expression are overall correlated, as both are increased under high nitrogen conditions. In some genes increased H3K4me2 was observed, also suggesting transcription. C : Chemical analysis of the product of the unique PKS19 gene cluster. The traces show the extracted ion chromatograms for [C 34 H 48 O 6 N 5 +H] + (first line) and [C 35 H 42 O 7 N 5 +H] + (second to fourth line) determined by HPLC-FTMS of an apicidin standard (first line) and of culture fluids from F. fujikuroi IMI58289, OE::APS8 and the OE::APS2/OE::APS8 mutant. D : UV spectra of apicidin and the apicidin-like compound. The similar spectra suggest a structural similarity.
    Figure Legend Snippet: Location of the NRPS/APS biosynthetic gene cluster on F. fujikuroi chromosome I, levels of histone modifications and gene expression within and flanking the cluster, and production of metabolites following overexpression of cluster genes APS2 and APS8 . A : Synteny between the apicidin gene cluster in F. semitectum [105] and the apicidin-like gene cluster in F. fujikuroi . B : Histone modifications and gene expression in and flanking the cluster. Histone marks are described in the legend to Figure 3 . Expression data were derived from microarray experiments in low and high nitrogen and are plotted as the changes in log 2 expression values in high-nitrogen medium compared to low-nitrogen medium. H3K9ac and gene expression are overall correlated, as both are increased under high nitrogen conditions. In some genes increased H3K4me2 was observed, also suggesting transcription. C : Chemical analysis of the product of the unique PKS19 gene cluster. The traces show the extracted ion chromatograms for [C 34 H 48 O 6 N 5 +H] + (first line) and [C 35 H 42 O 7 N 5 +H] + (second to fourth line) determined by HPLC-FTMS of an apicidin standard (first line) and of culture fluids from F. fujikuroi IMI58289, OE::APS8 and the OE::APS2/OE::APS8 mutant. D : UV spectra of apicidin and the apicidin-like compound. The similar spectra suggest a structural similarity.

    Techniques Used: Expressing, Over Expression, Derivative Assay, Microarray, High Performance Liquid Chromatography, Mutagenesis

    10) Product Images from "A Method to Determine 18O Kinetic Isotope Effects in the Hydrolysis of Nucleotide Triphosphates"

    Article Title: A Method to Determine 18O Kinetic Isotope Effects in the Hydrolysis of Nucleotide Triphosphates

    Journal: Analytical biochemistry

    doi: 10.1016/j.ab.2007.09.013

    Schematic diagram of the ThermoFinnigan Isolink interface. HPLC effluent is mixed with oxidation reagents through a T joint. The carbon source is oxidized quantitatively into CO 2 in a heated oxidation reactor. In the CO 2 separation unit, CO 2 escapes from
    Figure Legend Snippet: Schematic diagram of the ThermoFinnigan Isolink interface. HPLC effluent is mixed with oxidation reagents through a T joint. The carbon source is oxidized quantitatively into CO 2 in a heated oxidation reactor. In the CO 2 separation unit, CO 2 escapes from

    Techniques Used: High Performance Liquid Chromatography

    11) Product Images from "Water extract of Rumex crispus prevents bone loss by inhibiting osteoclastogenesis and inducing osteoblast mineralization"

    Article Title: Water extract of Rumex crispus prevents bone loss by inhibiting osteoclastogenesis and inducing osteoblast mineralization

    Journal: BMC Complementary and Alternative Medicine

    doi: 10.1186/s12906-017-1986-7

    HPLC-DAD chromatogram of WERC and three standard components (emodin, chrysophanol, and physcion) at wavelengths of 280 nm
    Figure Legend Snippet: HPLC-DAD chromatogram of WERC and three standard components (emodin, chrysophanol, and physcion) at wavelengths of 280 nm

    Techniques Used: High Performance Liquid Chromatography

    12) Product Images from "Quantitative HPLC-MS analysis of nucleotide sugars in plant cells following off-line SPE sample preparation"

    Article Title: Quantitative HPLC-MS analysis of nucleotide sugars in plant cells following off-line SPE sample preparation

    Journal: Analytical and Bioanalytical Chemistry

    doi: 10.1007/s00216-014-7746-3

    Influence of the potential applied to the ESI source on retention of UDP-sugars. Chromatogram obtained with a newly installed or regenerated PGC column ( a ); chromatogram obtained after 20-min utilization of the column connected to the ESI source of an Orbitrap mass spectrometer ( b ). Conditions: column regeneration was performed with 80 % acetonitrile in water containing 0.10 % trifluoroacetic acid for 20 min, the sample measurements were done with the gradient program described in the materials and methods section for HPLC-MS; detection, UV, 262 nm; sample. UDP-Gal (100 μmol L −1 ) and UDP-Glc (100 μmol L −1 )
    Figure Legend Snippet: Influence of the potential applied to the ESI source on retention of UDP-sugars. Chromatogram obtained with a newly installed or regenerated PGC column ( a ); chromatogram obtained after 20-min utilization of the column connected to the ESI source of an Orbitrap mass spectrometer ( b ). Conditions: column regeneration was performed with 80 % acetonitrile in water containing 0.10 % trifluoroacetic acid for 20 min, the sample measurements were done with the gradient program described in the materials and methods section for HPLC-MS; detection, UV, 262 nm; sample. UDP-Gal (100 μmol L −1 ) and UDP-Glc (100 μmol L −1 )

    Techniques Used: Pyrolysis Gas Chromatography, Mass Spectrometry, High Performance Liquid Chromatography, Gas Chromatography

    Influence of the potential applied to the ESI source on retention of UDP-sugars. Chromatogram obtained with a newly installed or regenerated PGC column ( a ); chromatogram obtained after 20-min utilization of the column connected to the ESI source of an Orbitrap mass spectrometer ( b ). Conditions: column regeneration was performed with 80 % acetonitrile in water containing 0.10 % trifluoroacetic acid for 20 min, the sample measurements were done with the gradient program described in the materials and methods section for HPLC-MS; detection, UV, 262 nm; sample. UDP-Gal (100 μmol L −1 ) and UDP-Glc (100 μmol L −1 )
    Figure Legend Snippet: Influence of the potential applied to the ESI source on retention of UDP-sugars. Chromatogram obtained with a newly installed or regenerated PGC column ( a ); chromatogram obtained after 20-min utilization of the column connected to the ESI source of an Orbitrap mass spectrometer ( b ). Conditions: column regeneration was performed with 80 % acetonitrile in water containing 0.10 % trifluoroacetic acid for 20 min, the sample measurements were done with the gradient program described in the materials and methods section for HPLC-MS; detection, UV, 262 nm; sample. UDP-Gal (100 μmol L −1 ) and UDP-Glc (100 μmol L −1 )

    Techniques Used: Pyrolysis Gas Chromatography, Mass Spectrometry, High Performance Liquid Chromatography, Gas Chromatography

    Analyte recoveries after liquid-liquid extraction ( a ) and solid-phase extraction ( b ). Recoveries were calculated from triplicate HPLC analysis of three to five replicate extractions of 10 μmol L −1 standard solution of the respective UDP-sugars. The diagram shows arithmetic averages and relative standard deviations of all analyses performed; sample size in ( a ): N = 9 for UDP-Glc, UDP-Xyl, UDP-GalA, and UDP-GlcA, N = 15 for UDP-Ara and UDP-Gal, sample size in ( b ): N = 31 for UDP-Glc, N = 15 for UDP-Ara, UDP-Xyl, UDP-Gal, UDP-GlcA, UDP-GalA
    Figure Legend Snippet: Analyte recoveries after liquid-liquid extraction ( a ) and solid-phase extraction ( b ). Recoveries were calculated from triplicate HPLC analysis of three to five replicate extractions of 10 μmol L −1 standard solution of the respective UDP-sugars. The diagram shows arithmetic averages and relative standard deviations of all analyses performed; sample size in ( a ): N = 9 for UDP-Glc, UDP-Xyl, UDP-GalA, and UDP-GlcA, N = 15 for UDP-Ara and UDP-Gal, sample size in ( b ): N = 31 for UDP-Glc, N = 15 for UDP-Ara, UDP-Xyl, UDP-Gal, UDP-GlcA, UDP-GalA

    Techniques Used: High Performance Liquid Chromatography, Gas Chromatography, Acetylene Reduction Assay

    13) Product Images from "Immunobiologic and Antiinflammatory Properties of a Bark Extract from Ampelozizyphus amazonicus Ducke"

    Article Title: Immunobiologic and Antiinflammatory Properties of a Bark Extract from Ampelozizyphus amazonicus Ducke

    Journal: BioMed Research International

    doi: 10.1155/2013/451679

    (a) HPLC-DAD chromatogram of SART (detection 210 nm) and betulinic acid at Rt = 8.387 min. (b) HPLC-DAD-ESI-MS (negative mode) chromatogram of SART. Triterpene zone between 5 and 10 min and saponin zone between 30 and 40 min (c) HPLC-DAD-ESI-MS chromatogram of SART: expansion of the saponin zone between 30 and 44 min.
    Figure Legend Snippet: (a) HPLC-DAD chromatogram of SART (detection 210 nm) and betulinic acid at Rt = 8.387 min. (b) HPLC-DAD-ESI-MS (negative mode) chromatogram of SART. Triterpene zone between 5 and 10 min and saponin zone between 30 and 40 min (c) HPLC-DAD-ESI-MS chromatogram of SART: expansion of the saponin zone between 30 and 44 min.

    Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry

    14) Product Images from "Analysis of pregnenolone and dehydroepiandrosterone in rodent brain: cholesterol autoxidation is the key"

    Article Title: Analysis of pregnenolone and dehydroepiandrosterone in rodent brain: cholesterol autoxidation is the key

    Journal: Journal of Lipid Research

    doi: 10.1194/jlr.M900162-JLR200

    Straight-phase HPLC on Lichrosorb Diol of a so-called steroid sulfate fraction from rat brain using the unreliable C18 SPE procedure. Each HPLC fraction was hydrolyzed and derivatized with HFBA at 20°C. The release of PREG (r-PREG) and DHEA (r-DHEA)
    Figure Legend Snippet: Straight-phase HPLC on Lichrosorb Diol of a so-called steroid sulfate fraction from rat brain using the unreliable C18 SPE procedure. Each HPLC fraction was hydrolyzed and derivatized with HFBA at 20°C. The release of PREG (r-PREG) and DHEA (r-DHEA)

    Techniques Used: High Performance Liquid Chromatography

    15) Product Images from "Different effects of feeding pregnant and lactating mice Rhodiola kirilowii aqueous and hydro-alcoholic extracts on their serum angiogenic activity and content of selected polyphenols"

    Article Title: Different effects of feeding pregnant and lactating mice Rhodiola kirilowii aqueous and hydro-alcoholic extracts on their serum angiogenic activity and content of selected polyphenols

    Journal: Central-European Journal of Immunology

    doi: 10.5114/ceji.2017.67314

    A) HPLC analysis of extracts. Statistical differences between RKW and RKW-A extracts: salidroside (1) p
    Figure Legend Snippet: A) HPLC analysis of extracts. Statistical differences between RKW and RKW-A extracts: salidroside (1) p

    Techniques Used: High Performance Liquid Chromatography

    16) Product Images from "Polydiacetylene-Based High-Throughput Screen for Surfactin Producing Strains of Bacillus subtilis"

    Article Title: Polydiacetylene-Based High-Throughput Screen for Surfactin Producing Strains of Bacillus subtilis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0088207

    Strategy for screening surfactin producers. (Step1) Bacterial suspension of B. sbtilis 723 is mutated by ARTP; (Step 2) Mutants after gradient dilution are plated on LB agar at 37°C until clear individual colonies are observed (12 h); (Step 3) Select individual colonies to MicroFlask by Duetz and incubate aerobically at 37°C, 200 rpm for 24 h; (Step 4) The MicroFlask by Duetz with fermentation broths are centrifuged at 8000× g for 10 min; (Step 5) 150 µL supernatant and 75 µL PDA vesicles are reacted at 25°C for 10 min, and the CR% values are calculated; (Step 6) The strains with CR% more than 26% are preserved; (Step 7) The high-yielding mutants are cultivated under conditions appropriate for surfactin production (LB medium, 37°C), and (Step 8) The cell-free culture supernatant is collected and analyzed by HPLC to quantify the surfactin content in the medium.
    Figure Legend Snippet: Strategy for screening surfactin producers. (Step1) Bacterial suspension of B. sbtilis 723 is mutated by ARTP; (Step 2) Mutants after gradient dilution are plated on LB agar at 37°C until clear individual colonies are observed (12 h); (Step 3) Select individual colonies to MicroFlask by Duetz and incubate aerobically at 37°C, 200 rpm for 24 h; (Step 4) The MicroFlask by Duetz with fermentation broths are centrifuged at 8000× g for 10 min; (Step 5) 150 µL supernatant and 75 µL PDA vesicles are reacted at 25°C for 10 min, and the CR% values are calculated; (Step 6) The strains with CR% more than 26% are preserved; (Step 7) The high-yielding mutants are cultivated under conditions appropriate for surfactin production (LB medium, 37°C), and (Step 8) The cell-free culture supernatant is collected and analyzed by HPLC to quantify the surfactin content in the medium.

    Techniques Used: High Performance Liquid Chromatography

    HPLC profile of surfactin produced by parent strain and mutant BS-37. Parent strain B. subtilis 723 and mutant BS-37 were cultured in 250 mL Erlenmeyer flask containing 50 mL LB medium aerobically at 37°C and 200 rpm for 24 h. The products were extracted by acid centrifugation and methanol extraction. The elution was monitored at 214 nm at a flow rate of 0.8 mL/min. The red line and the black line represent the HPLC profiles of surfactins produced by B. subtilis 723 and BS-37, respectively.
    Figure Legend Snippet: HPLC profile of surfactin produced by parent strain and mutant BS-37. Parent strain B. subtilis 723 and mutant BS-37 were cultured in 250 mL Erlenmeyer flask containing 50 mL LB medium aerobically at 37°C and 200 rpm for 24 h. The products were extracted by acid centrifugation and methanol extraction. The elution was monitored at 214 nm at a flow rate of 0.8 mL/min. The red line and the black line represent the HPLC profiles of surfactins produced by B. subtilis 723 and BS-37, respectively.

    Techniques Used: High Performance Liquid Chromatography, Produced, Mutagenesis, Cell Culture, Centrifugation, Flow Cytometry

    17) Product Images from "A comprehensive method for lipid profiling by liquid chromatography-ion cyclotron resonance mass spectrometry [S]"

    Article Title: A comprehensive method for lipid profiling by liquid chromatography-ion cyclotron resonance mass spectrometry [S]

    Journal: Journal of Lipid Research

    doi: 10.1194/jlr.D016550

    Total ion chromatogram of lipids from a representative lipid droplet sample acquired by reversed-phase-HPLC/LTQ-FT. A reversed-phase C18 column is used for separation of lipid species and monitored in positive ESI mode. The scan range of the LTQ-FT is m/z 350-1,050.
    Figure Legend Snippet: Total ion chromatogram of lipids from a representative lipid droplet sample acquired by reversed-phase-HPLC/LTQ-FT. A reversed-phase C18 column is used for separation of lipid species and monitored in positive ESI mode. The scan range of the LTQ-FT is m/z 350-1,050.

    Techniques Used: High Performance Liquid Chromatography

    18) Product Images from "Dried Blood Spot Proteomics: Surface Extraction of Endogenous Proteins Coupled with Automated Sample Preparation and Mass Spectrometry Analysis"

    Article Title: Dried Blood Spot Proteomics: Surface Extraction of Endogenous Proteins Coupled with Automated Sample Preparation and Mass Spectrometry Analysis

    Journal: Journal of the American Society for Mass Spectrometry

    doi: 10.1007/s13361-013-0658-1

    Summary of robotic workflow. (a) Starting position: DBS is mounted on the microtitre plate. One well contains extraction solvent and a second well contains trypsin solution; (b) 7 μL of 50 mMol NH 4 HCO 3 is aspirated from solvent well; (c) 6 μL is dispensed onto DBS surface. Liquid microjunction is maintained between the pipette tip and the DBS surface (for 4 s) allowing intact proteins to dissolve into solvent; (d) solution of intact proteins (5 μL) is re-aspirated and dispensed into clean sample well; (e) 4.5 μL of 0.1 μg/μL trypsin solution is aspirated from trypsin well; (f) trypsin solution is added to sample well; (g) sample is incubated at 40 °C for 1 h. Enzyme digests intact proteins into peptides; (h) and (i) as solvent begins to evaporate from sample well, additional solvent (7.5 μL) is aspirated from solvent well and added to sample well [ (h) and (i) are performed at 30 min and 1 h]. (j) Proteins are digested into peptides after 1 h. (k) Plate is transferred to HPLC autosampler and peptides are analyzed by LC MS/MS
    Figure Legend Snippet: Summary of robotic workflow. (a) Starting position: DBS is mounted on the microtitre plate. One well contains extraction solvent and a second well contains trypsin solution; (b) 7 μL of 50 mMol NH 4 HCO 3 is aspirated from solvent well; (c) 6 μL is dispensed onto DBS surface. Liquid microjunction is maintained between the pipette tip and the DBS surface (for 4 s) allowing intact proteins to dissolve into solvent; (d) solution of intact proteins (5 μL) is re-aspirated and dispensed into clean sample well; (e) 4.5 μL of 0.1 μg/μL trypsin solution is aspirated from trypsin well; (f) trypsin solution is added to sample well; (g) sample is incubated at 40 °C for 1 h. Enzyme digests intact proteins into peptides; (h) and (i) as solvent begins to evaporate from sample well, additional solvent (7.5 μL) is aspirated from solvent well and added to sample well [ (h) and (i) are performed at 30 min and 1 h]. (j) Proteins are digested into peptides after 1 h. (k) Plate is transferred to HPLC autosampler and peptides are analyzed by LC MS/MS

    Techniques Used: Transferring, Incubation, High Performance Liquid Chromatography, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry

    19) Product Images from "Isaria tenuipes Peck, an entomopathogenic fungus from Darjeeling Himalaya: Evaluation of in-vitro antiproliferative and antioxidant potential of its mycelium extract"

    Article Title: Isaria tenuipes Peck, an entomopathogenic fungus from Darjeeling Himalaya: Evaluation of in-vitro antiproliferative and antioxidant potential of its mycelium extract

    Journal: BMC Complementary Medicine and Therapies

    doi: 10.1186/s12906-020-02973-w

    a HPLC profile representing signal generated at 210nm by injecting 20 μl of 20μg/ml beauvericin (BEA) standard; b methanol extract from mycelia
    Figure Legend Snippet: a HPLC profile representing signal generated at 210nm by injecting 20 μl of 20μg/ml beauvericin (BEA) standard; b methanol extract from mycelia

    Techniques Used: High Performance Liquid Chromatography, Generated

    20) Product Images from "Phosphoproteomics of short-term hedgehog signaling in human medulloblastoma cells"

    Article Title: Phosphoproteomics of short-term hedgehog signaling in human medulloblastoma cells

    Journal: Cell Communication and Signaling : CCS

    doi: 10.1186/s12964-020-00591-0

    Scheme of experimental design ( a ) and analytical workflow. a Three biological replicates of human medulloblastoma cells were treated with DMSO, SAG, vismodegib or EGF in starving medium for 5.0 and 15 min. b Analytical workflow: After treatment, sample preparation, and TMT 10-plex labeling, peptides of each time point and treatment were pooled. Aliquots were utilized for pH 8 fractionation and deep proteome profiling or phosphopeptide enrichment by metal oxide affinity chromatography. Measurements of peptides and phosphopeptides were conducted with reversed-phase HPLC coupled to high-resolution mass spectrometry
    Figure Legend Snippet: Scheme of experimental design ( a ) and analytical workflow. a Three biological replicates of human medulloblastoma cells were treated with DMSO, SAG, vismodegib or EGF in starving medium for 5.0 and 15 min. b Analytical workflow: After treatment, sample preparation, and TMT 10-plex labeling, peptides of each time point and treatment were pooled. Aliquots were utilized for pH 8 fractionation and deep proteome profiling or phosphopeptide enrichment by metal oxide affinity chromatography. Measurements of peptides and phosphopeptides were conducted with reversed-phase HPLC coupled to high-resolution mass spectrometry

    Techniques Used: Sample Prep, Labeling, Fractionation, Affinity Chromatography, High Performance Liquid Chromatography, Mass Spectrometry

    21) Product Images from "Overexpression of artificially fused bifunctional enzyme 4CL1–CCR: a method for production of secreted 4-hydroxycinnamaldehydes in Escherichia coli"

    Article Title: Overexpression of artificially fused bifunctional enzyme 4CL1–CCR: a method for production of secreted 4-hydroxycinnamaldehydes in Escherichia coli

    Journal: Microbial Cell Factories

    doi: 10.1186/s12934-015-0309-2

    HPLC–PDA separation and ESI-Ion-trap-MS detection of phenylpropanoic acids (substrates) and 4-hydrocinnaldehydes (products). From left to right caffeic acid, caffealdehyde, p -coumaric acid, ferulic acid, p -coumaraldhyde, coniferaldehyde and sinapaldehyde.
    Figure Legend Snippet: HPLC–PDA separation and ESI-Ion-trap-MS detection of phenylpropanoic acids (substrates) and 4-hydrocinnaldehydes (products). From left to right caffeic acid, caffealdehyde, p -coumaric acid, ferulic acid, p -coumaraldhyde, coniferaldehyde and sinapaldehyde.

    Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry

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  • 93
    Thermo Fisher analysis hplc separations
    Multivariate statistical analysis of <t>HPLC–PDA–MS</t> non-targeted profiles. a Conventional PCA scores plot inclusive of quality assurance samples. b Conventional PCA scores plot with quality assurance samples excluded. c Conventional PCA loadings plot with quality assurance samples excluded (please refer to Table S1 and S2 for metabolites associated with unique reference numbers). d Multiblock hierarchical (H)PCA super-scores plot. e Multiblock hierarchical (H)PCA block-scores plots based upon daylength condition. Natural, 10 h, 10 h + 3 h, refer to the following daylength condition descriptions, (1) natural long summer day (LD), ca. 18 h (natural LD), (2) 10 h artificial short day (SD), and (3) 10 h SD + 3 h night interruption (SD + NI), respectively
    Analysis Hplc Separations, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher hplc esi it msn analyses
    Total ion current chromatogram of the methanol extract of scapes of P. chiquitensis <t>(HPLC-ESI-IT-MS</t> n negative ion mode). For conditions, see experimental part.
    Hplc Esi It Msn Analyses, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher hplc ms ms
    Genotoxicity of PhIP in Apc +/+ and Apc Min/+ cell lines. Apc +/+ and Apc Min/+ cells were treated with the indicated concentrations of PhIP for 24 h. (A) Genotoxicity was evaluated by <t>HPLC-MS/MS</t> quantification of <t>dG-C8-PhIP</t> in DNA. (B) Genotoxicity was evaluated with γHAX ICW assay. Bars represent the average of at least three independent experiments with SEM. Statistically significant increase in H2AX phosphorylation compared with DMSO control using Student’s test; *, p
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    Thermo Fisher hplc fraction
    Characterization of active components of MBC extract. (a, b) <t>HPLC</t> fractionation of MBC extract. MBC extract was fractioned by HPLC, and each fraction was screened for activity in inhibiting endotoxin-induced <t>HMGB1</t> release. Note that two major UV absorption peaks (I and II) were detected at the wavelengths of 254, 280, and 360 nm (a) and coeluted with the activities to inhibit endotoxin-induced HMGB1 release (b). (c, d) Mass spectrometry analysis of HPLC peaks. The MS spectrum of the MBC HPLC peak II was identical to that of the commercially obtained isovitexin standard.
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    Multivariate statistical analysis of HPLC–PDA–MS non-targeted profiles. a Conventional PCA scores plot inclusive of quality assurance samples. b Conventional PCA scores plot with quality assurance samples excluded. c Conventional PCA loadings plot with quality assurance samples excluded (please refer to Table S1 and S2 for metabolites associated with unique reference numbers). d Multiblock hierarchical (H)PCA super-scores plot. e Multiblock hierarchical (H)PCA block-scores plots based upon daylength condition. Natural, 10 h, 10 h + 3 h, refer to the following daylength condition descriptions, (1) natural long summer day (LD), ca. 18 h (natural LD), (2) 10 h artificial short day (SD), and (3) 10 h SD + 3 h night interruption (SD + NI), respectively

    Journal: Metabolomics

    Article Title: Application of HPLC–PDA–MS metabolite profiling to investigate the effect of growth temperature and day length on blackcurrant fruit

    doi: 10.1007/s11306-018-1462-5

    Figure Lengend Snippet: Multivariate statistical analysis of HPLC–PDA–MS non-targeted profiles. a Conventional PCA scores plot inclusive of quality assurance samples. b Conventional PCA scores plot with quality assurance samples excluded. c Conventional PCA loadings plot with quality assurance samples excluded (please refer to Table S1 and S2 for metabolites associated with unique reference numbers). d Multiblock hierarchical (H)PCA super-scores plot. e Multiblock hierarchical (H)PCA block-scores plots based upon daylength condition. Natural, 10 h, 10 h + 3 h, refer to the following daylength condition descriptions, (1) natural long summer day (LD), ca. 18 h (natural LD), (2) 10 h artificial short day (SD), and (3) 10 h SD + 3 h night interruption (SD + NI), respectively

    Article Snippet: Untargeted HPLC–PDA–MS analysis HPLC separations were performed with a Thermo Accela 600 HPLC system coupled with an Accela PDA detector (Thermo-Fisher Ltd. UK).

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry, Blocking Assay

    Total ion current chromatogram of the methanol extract of scapes of P. chiquitensis (HPLC-ESI-IT-MS n negative ion mode). For conditions, see experimental part.

    Journal: Molecules

    Article Title: Characterization of Flavonoids and Naphthopyranones in Methanol Extracts of Paepalanthus chiquitensis Herzog by HPLC-ESI-IT-MSn and Their Mutagenic Activity

    doi: 10.3390/molecules18010244

    Figure Lengend Snippet: Total ion current chromatogram of the methanol extract of scapes of P. chiquitensis (HPLC-ESI-IT-MS n negative ion mode). For conditions, see experimental part.

    Article Snippet: HPLC-ESI-IT-MSn Analyses The methanol extracts of P. chiquitensis (capitulae and scapes) were analyzed separately by in-line HPLC-ESI-IT-MSn , using a SURVEYOR MS micro system coupled in-line to an LCQ Fleet ion-trap mass spectrometer (Thermo Scientific).

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry

    Total ion current chromatogram of the methanol extract of capitulae of P. chiquitensis (HPLC-ESI-IT-MS n negative ion mode). For conditions, see experimental part.

    Journal: Molecules

    Article Title: Characterization of Flavonoids and Naphthopyranones in Methanol Extracts of Paepalanthus chiquitensis Herzog by HPLC-ESI-IT-MSn and Their Mutagenic Activity

    doi: 10.3390/molecules18010244

    Figure Lengend Snippet: Total ion current chromatogram of the methanol extract of capitulae of P. chiquitensis (HPLC-ESI-IT-MS n negative ion mode). For conditions, see experimental part.

    Article Snippet: HPLC-ESI-IT-MSn Analyses The methanol extracts of P. chiquitensis (capitulae and scapes) were analyzed separately by in-line HPLC-ESI-IT-MSn , using a SURVEYOR MS micro system coupled in-line to an LCQ Fleet ion-trap mass spectrometer (Thermo Scientific).

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry

    Genotoxicity of PhIP in Apc +/+ and Apc Min/+ cell lines. Apc +/+ and Apc Min/+ cells were treated with the indicated concentrations of PhIP for 24 h. (A) Genotoxicity was evaluated by HPLC-MS/MS quantification of dG-C8-PhIP in DNA. (B) Genotoxicity was evaluated with γHAX ICW assay. Bars represent the average of at least three independent experiments with SEM. Statistically significant increase in H2AX phosphorylation compared with DMSO control using Student’s test; *, p

    Journal: PLoS ONE

    Article Title: Combined Genotoxic Effects of a Polycyclic Aromatic Hydrocarbon (B(a)P) and an Heterocyclic Amine (PhIP) in Relation to Colorectal Carcinogenesis

    doi: 10.1371/journal.pone.0058591

    Figure Lengend Snippet: Genotoxicity of PhIP in Apc +/+ and Apc Min/+ cell lines. Apc +/+ and Apc Min/+ cells were treated with the indicated concentrations of PhIP for 24 h. (A) Genotoxicity was evaluated by HPLC-MS/MS quantification of dG-C8-PhIP in DNA. (B) Genotoxicity was evaluated with γHAX ICW assay. Bars represent the average of at least three independent experiments with SEM. Statistically significant increase in H2AX phosphorylation compared with DMSO control using Student’s test; *, p

    Article Snippet: The dG-C8-PhIP adducts were quantified by HPLC-MS/MS using of a Surveyor HPLC pump (Thermo Scientific, Les Ullis, France) associated with a TSQ Quantum Discovery Max triple quadrupole (Thermo Scientific, Les Ullis, France).

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry

    Synergic genotoxicity of B( a )P and PhIP in Apc +/+ and Apc Min/+ cell lines. Apc +/+ and Apc Min/+ cells were treated with the indicated concentrations of BaP and PhIP for 24 h. (A) Genotoxicity was evaluated by quantification by HPLC-MS/MS quantification of B( a )P- and PhIP-derived DNA adducts. (B) Genotoxicity was evaluated with γHAX ICW assay. Bars represent the average of at least three independent experiments with SEM. Statistically significant increase in H2AX phosphorylation compared with DMSO control using Student’s test; **, p

    Journal: PLoS ONE

    Article Title: Combined Genotoxic Effects of a Polycyclic Aromatic Hydrocarbon (B(a)P) and an Heterocyclic Amine (PhIP) in Relation to Colorectal Carcinogenesis

    doi: 10.1371/journal.pone.0058591

    Figure Lengend Snippet: Synergic genotoxicity of B( a )P and PhIP in Apc +/+ and Apc Min/+ cell lines. Apc +/+ and Apc Min/+ cells were treated with the indicated concentrations of BaP and PhIP for 24 h. (A) Genotoxicity was evaluated by quantification by HPLC-MS/MS quantification of B( a )P- and PhIP-derived DNA adducts. (B) Genotoxicity was evaluated with γHAX ICW assay. Bars represent the average of at least three independent experiments with SEM. Statistically significant increase in H2AX phosphorylation compared with DMSO control using Student’s test; **, p

    Article Snippet: The dG-C8-PhIP adducts were quantified by HPLC-MS/MS using of a Surveyor HPLC pump (Thermo Scientific, Les Ullis, France) associated with a TSQ Quantum Discovery Max triple quadrupole (Thermo Scientific, Les Ullis, France).

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry, Derivative Assay

    Characterization of active components of MBC extract. (a, b) HPLC fractionation of MBC extract. MBC extract was fractioned by HPLC, and each fraction was screened for activity in inhibiting endotoxin-induced HMGB1 release. Note that two major UV absorption peaks (I and II) were detected at the wavelengths of 254, 280, and 360 nm (a) and coeluted with the activities to inhibit endotoxin-induced HMGB1 release (b). (c, d) Mass spectrometry analysis of HPLC peaks. The MS spectrum of the MBC HPLC peak II was identical to that of the commercially obtained isovitexin standard.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: It Is Not Just Folklore: The Aqueous Extract of Mung Bean Coat Is Protective against Sepsis

    doi: 10.1155/2012/498467

    Figure Lengend Snippet: Characterization of active components of MBC extract. (a, b) HPLC fractionation of MBC extract. MBC extract was fractioned by HPLC, and each fraction was screened for activity in inhibiting endotoxin-induced HMGB1 release. Note that two major UV absorption peaks (I and II) were detected at the wavelengths of 254, 280, and 360 nm (a) and coeluted with the activities to inhibit endotoxin-induced HMGB1 release (b). (c, d) Mass spectrometry analysis of HPLC peaks. The MS spectrum of the MBC HPLC peak II was identical to that of the commercially obtained isovitexin standard.

    Article Snippet: Each HPLC fraction was screened for HMGB1-inhibiting activities, and the components of the active HPLC fraction were analyzed by mass spectrometry using a triple quadrupole mass spectrometer (Thermo TSQ Quantum Access, Thermo-Fisher).

    Techniques: High Performance Liquid Chromatography, Fractionation, Activity Assay, Mass Spectrometry